Freshwater Mussel Survey in Pool 16, the Mississippi River, Near Fairport, Iowa: R.M

FRESHWATER MUSSEL SURVEY IN POOL 16, THE MISSISSIPPI RIVER, NEAR FAIRPORT, IOWA: R.M. 463.5 – R.M. 464.1, APPROXIMATELY

Tatsuaki Nakato Lucille A. Carver Mississippi Riverside Environmental Research Station (LACMRERS)

Jon Christensen and Bernard Schonhoff Iowa DNR Fairport Fisheries Management

IIHR Technical Report No. 464

IIHR − Hydroscience & Engineering College of Engineering The University of Iowa Iowa City, Iowa 52242-1585

August 2007

ABSTRACT

As part of the Mussel Coordination Team’s 2007 Higgins eye pearly mussel (Lampsilis higginsii) relocation plan, Lucille A. Carver Mississippi Riverside Environmental Research Station (LACMRERS), The University of Iowa, and the Iowa DNR Fairport Fisheries Management recommended that juvenile Higgins eye pearly mussels be released in the Mississippi River near Fairport, Iowa in Pool 16. Such a proposed action must fulfill regulatory requirements stipulated under the provisions of the federal Endangered Species Act (ESA) of 1973, as amended and associated regulations. A multi-agency team, comprising the Illinois DNR, the Iowa DNR, the U.S. Army Corps of Engineers Rock Island District, and LACMRERS, conducted detailed quantitative field surveys on the mussel population and its diversity during 12-13 September 2006. Sixteen different live freshwater mussel species were found in the mussel survey area along the right bank of the Mississippi River near Fairport, extending riverward approximately 160 m from the Iowa shoreline and encompassing streamwise approximately 800 m. The most common species found out of 253 live mussels sampled was Threeridge (Amblema plicata: 17.8%) followed by Pimpleback (Quadrula pustulosa: 17.4%), Threehorn wartyback (Obliquaria reflexa: 15.8%), Washboard (Megalonaias nervosa: 12.3%), and Mapleleaf (Quadrula quadrula: 12.3%). Frequencies of the other eleven species were less than 6.3%. The highest age count occurred at age four (55 counts), followed by age six (43 counts) and age five (40 counts). An age group between 4-yrs old and 6-yrs old occupied 54.5% of the total population; that between 3-yrs old and 7-yrs old 78.2%; that between 2-yrs old and 9-yrs old 97.5%; and that between 1-yr old and 10-yrs old 99.6%. High live-mussel population densities were found in three quadrat samples out of five samples, where mussel densities were 26, 30, and 31 mussels/m2, respectively. Mussel densities at the other two sites were 7 and 8 mussels/m2, respectively.

ACKNOWLEDGMENTS

The field mussel-survey investigation reported herein was conducted in cooperation with multiple agencies, including the Illinois Department of Natural Resources (ILDNR), the Iowa Department of Natural Resources (IADNR), the U. S. Army Corps of Engineers, Rock Island District (COE-RID), and Lucille A. Carver Mississippi Riverside Environmental Research Station (LACMRERS), The University of Iowa. The authors thank Bob Schanzle, Rich Lewis, and Ed Walsh of the ILDNR; Eric Giebelstein of the IADNR; and Steve Johnson and Nicole McVay of the COE-RID for their unfailing cooperation throughout the course of the field surveys. The authors also thank Bob Schanzle for reviewing the manuscript. Bob Schanzle and Rich Lewis were principal divers to collect freshwater mussel samples.

ii TABLE OF CONTENTS

Page I. INTRODUCTION 1 1. Background 1 2. Study Objectives 1 3. Mussel Survey Sites 2 4. Survey Methods 7 II. PRESENTATION OF INDIVIDUAL SURVEY RESULTS 8 1. Pollywog 1 (UTM: NAD 27 and Zone 15 - 676074E/4589311N) 8 2. Pollywog 2 (UTM: NAD 27 and Zone 15 - 676068E/4589298N) 9 3. Dive 1 (UTM: NAD 27 and Zone 15 - 676063E/4589286N) 10 4. Dive 2 (UTM: NAD 27 and Zone 15 - 676089E/4589249N) 14 5. Dive 3 (UTM: NAD 27 and Zone 15 - 6760809E/4589279N) 16 6. Dive 4 (UTM: NAD 27 and Zone 15 - 675997E/4589124N) 18 7. Dive 5 (UTM: NAD 27 and Zone 15 - 6759617E/4589122N) 20 8. Dive 6 (UTM: NAD 27 and Zone 15 - 675974E/4589183N) 22 9. Dive 7 (UTM: NAD 27 and Zone 15 - 676191E/4589397N) 24 10. Dive 8 (UTM: NAD 27 and Zone 15 - 676209E/4589372N) 27 11. Dive 9 (UTM: NAD 27 and Zone 15 - 676245E/4589311N) 29 12. Dive 10 (UTM: NAD 27 and Zone 15 - 676301E/4589473N) 31 13. Dive 11 (UTM: NAD 27 and Zone 15 - 676348E/4589415N) 33 14. Dive 12 (UTM: NAD 27 and Zone 15 - 676441E/4589463N) 35 15. Dive 13 (UTM: NAD 27 and Zone 15 - 676413E/4589523N) 37 16. Dive 14 (UTM: NAD 27 and Zone 15 - 676542E/4589650N) 39 17. Dive 15 (UTM: NAD 27 and Zone 15 - 676566E/4589591N) 41 18. Quadrat 1-A (UTM: NAD 27 and Zone 15 - 676567E/4589650N) 43 19. Quadrat 1-B (UTM: NAD 27 and Zone 15 - 676567E/4589650N) 43 20. Quadrat 1-C (UTM: NAD 27 and Zone 15 - 676567E/4589650N) 43 21. Quadrat 1-D (UTM: NAD 27 and Zone 15 - 676567E/4589650N) 47 22. Quadrat 2-A (UTM: NAD 27 and Zone 15 - 676324E/4589471N) 47 23. Quadrat 2-B (UTM: NAD 27 and Zone 15 - 676324E/4589471N) 47 24. Quadrat 2-C (UTM: NAD 27 and Zone 15 - 676324E/4589471N) 51 25. Quadrat 2-D (UTM: NAD 27 and Zone 15 - 676324E/4589471N) 51 26. Quadrat 3-A (UTM: NAD 27 and Zone 15 - 676400E/4589532N) 51 27. Quadrat 3-B (UTM: NAD 27 and Zone 15 - 676400E/4589532N) 55 28. Quadrat 3-C (UTM: NAD 27 and Zone 15 - 676400E/4589532N) 55 29. Quadrat 3-D (UTM: NAD 27 and Zone 15 - 676400E/4589532N) 55 30. Quadrat 4-A (UTM: NAD 27 and Zone 15 - 676343E/4589456N) 59 31. Quadrat 4-B (UTM: NAD 27 and Zone 15 - 676343E/4589456N) 59 32. Quadrat 4-C (UTM: NAD 27 and Zone 15 - 676343E/4589456N) 59 33. Quadrat 4-D (UTM: NAD 27 and Zone 15 - 676343E/4589456N) 59 34. Quadrat 5-A (UTM: NAD 27 and Zone 15 - 676218E/4589403N) 64 35. Quadrat 5-B (UTM: NAD 27 and Zone 15 - 676218E/4589403N) 64 36. Quadrat 5-C (UTM: NAD 27 and Zone 15 - 676218E/4589403N) 64

iii 37. Quadrat 5-D (UTM: NAD 27 and Zone 15 - 676218E/4589403N) 64 38. Muskrat Midden Site (UTM: NAD 27 and Zone 15 - 676418E/4589598N) 69 III. SUMMARY OF OVERALL SURVEY RESULTS 71 IV. REFERENCES 77

LIST OF TABLES

Page Table 1-1 GPS locations and flow depths at individual data sampling sites 6 Table 2-1 Size and age distributions of live mussels collected during Pollywog 1 9 Table 2-2 Size and age distributions of live mussels collected during Pollywog 2 11 Table 2-3 Size and age distributions of live mussels collected during Dive 1 13 Table 2-4 Size and age distributions of live mussels collected during Dive 2 15 Table 2-5 Size and age distributions of live mussels collected during Dive 3 17 Table 2-6 Size and age distributions of live mussels collected during Dive 4 19 Table 2-7 Size and age distributions of live mussels collected during Dive 5 21 Table 2-8 Size and age distributions of live mussels collected during Dive 6 23 Table 2-9 Size and age distributions of live mussels collected during Dive 7 26 Table 2-10 Size and age distributions of live mussels collected during Dive 8 28 Table 2-11 Size and age distributions of live mussels collected during Dive 9 30 Table 2-12 Size and age distributions of live mussels collected during Dive 10 32 Table 2-13 Size and age distributions of live mussels collected during Dive 11 34 Table 2-14 Size and age distributions of live mussels collected during Dive 12 36 Table 2-15 Size and age distributions of live mussels collected during Dive 13 38 Table 2-16 Size and age distributions of live mussels collected during Dive 14 40 Table 2-17 Size and age distributions of live mussels collected during Dive 15 42 Table 2-18 Size and age distributions of live mussels collected in Quadrat 1-A 44 Table 2-19 Size and age distributions of live mussels collected in Quadrat 1-B 45 Table 2-20 Size and age distributions of live mussels collected in Quadrat 1-C 46 Table 2-21 Size and age distributions of live mussels collected in Quadrat 1-D 48 Table 2-22 Size and age distributions of live mussels collected in Quadrat 2-A 49 Table 2-23 Size and age distributions of live mussels collected in Quadrat 2-B 50 Table 2-24 Size and age distributions of live mussels collected in Quadrat 2-C 52 Table 2-25 Size and age distributions of live mussels collected in Quadrat 2-D 53 Table 2-26 Size and age distributions of live mussels collected in Quadrat 3-A 54 Table 2-27 Size and age distributions of live mussels collected in Quadrat 3-B 56 Table 2-28 Size and age distributions of live mussels collected in Quadrat 3-C 57 Table 2-29 Size and age distributions of live mussels collected in Quadrat 3-D 58 Table 2-30 Size and age distributions of live mussels collected in Quadrat 4-A 60 Table 2-31 Size and age distributions of live mussels collected in Quadrat 4-B 61 Table 2-32 Size and age distributions of live mussels collected in Quadrat 4-C 62 Table 2-33 Size and age distributions of live mussels collected in Quadrat 4-D 63 Table 2-34 Size and age distributions of live mussels collected in Quadrat 5-A 65 Table 2-35 Size and age distributions of live mussels collected in Quadrat 5-B 66

iv Table 2-36 Size and age distributions of live mussels collected in Quadrat 5-C 67 Table 2-37 Size and age distributions of live mussels collected in Quadrat 5-D 68 Table 2-38 List of mussel species found on 14 September 2006 at a muskrat midden located just upstream from Quadrat 3 site on the right bank of the Mississippi River 69 Table 3-1 Summary of live mussels and substrate materials collected 73 Table 3-2 Summary of age counts of live mussels at individual survey sites 73 Table 3-3 Summary of live mussels collected by five Quadrat surveys 74

LIST OF FIGURES

Page Figure 1-1 General mussel survey area near Fairport, Iowa (U.S.G.S. topographic map powered by TOPO! published by National Geographic) 4 Figure 1-2 A close-up map showing mussel data collection sites near Fairport, Iowa (2000 color infrared digital orthophoto shown was downloaded from the Iowa State University’s Iowa Geographic Map Server site: http://komodo.gis.iastate.edu/) 5 Figure 1-3 A close-up map identifying mussel data collection sites with local flow depths near Fairport, Iowa (2000 color infrared digital orthophoto shown was downloaded from the Iowa State University’s Iowa Geographic Map Server site: http://komodo.gis.iastate.edu/) 5 Figure 3-1 Frequency distribution of sixteen live mussel species 75 Figure 3-2 Percentage distribution of sixteen live mussel species 75 Figure 3-3 Age distribution of 253 live mussels 76 Figure 3-4 Daily Mississippi River stage records near Fairport, Iowa from 1 January 1990 to 30 August 2007 – data source: http://www2.mvr.usace.army.mil/WaterControl/new/layout.cfm 76

LIST OF PHOTOS

Page

Photo 1-1 Male and female Higgins eye (Lampsilis higginsii) pearly mussels: male’s exterior (a), male’s pearly interior (b), female’s exterior (c), and female’s interior (d) 2 Photo 1-2 Higgins eye (Lampsilis higginsii) pearly mussels infested by zebra mussels (Dreissena polymorpha) (a), and those zebra-free LHs after removing zebra mussels – photos were taken on 1 August 2007 during MCT’s mussel cleaning effort near Cordova, IL in the Mississippi River 3 Photo 1-3 Juvenile Higgins eye (Lampsilis higginsii) pearly mussels raised in a cage in Lake Pepin near Lake City, Minnesota by the U.S. Fish

v & Wildlife Service (a), and a close-up view of 2-yr old Higgins eye mussels (b) – notice some zebra mussels attached to Higgins eye mussels 3 Photo 1-4 The mouth of an unnamed creek (top center) – flow direction from right to left 6 Photo 1-5 Brailing operation prior to mussel surveys 7 Photo 1-6 Species identifications for a quadrat sample 7 Photo 2-1 Nine live mussels collected in Pollywog 1 8 Photo 2-2 Five live mussels collected in Pollywog 2 10 Photo 2-3 Seven live mussels collected in Dive 1 12 Photo 2-4 Mucket (Actinonaias ligamentina) collected in Dive 1 (anterior: right and posterior: left) 12 Photo 2-5 Ten live mussels collected in Dive 2 14 Photo 2-6 Fourteen live mussels collected in Dive 3 16 Photo 2-7 Nine live mussels collected in Dive 4 18 Photo 2-8 Two live mussels collected in Dive 5 20 Photo 2-9 Ten live mussels collected in Dive 6 22 Photo 2-10 Eleven live mussels collected in Dive 7 24 Photo 2-11 Mucket (Actinonaias ligamentina) collected in Dive 7 anterior: right and posterior: left) 25 Photo 2-12 Mucket (Actinonaias ligamentina) collected in Dive 7 (anterior: left and posterior: right) 25 Photo 2-13 Eighteen live mussels collected in Dive 8 27 Photo 2-14 Two live mussels collected in Dive 9 29 Photo 2-15 Six live mussels collected in Dive 10 31 Photo 2-16 Eight live mussels collected in Dive 11 33 Photo 2-17 Six live mussels collected in Dive 12 35 Photo 2-18 Thirteen live mussels collected in Dive 13 37 Photo 2-19 Sixteen live mussels collected in Dive 14 39 Photo 2-20 Five live mussels collected in Dive 15 41 Photo 2-21 Seventeen different dead mussel shells found in a muskrat midden in Fairport (UTM Datum, NAD 27, Zone 15: 676418E/4589598N) 70

vi FRESHWATER MUSSEL SURVEY IN POOL 16, THE MISSISSIPPI RIVER, NEAR FAIRPORT, IOWA: R.M. 463.5 – R.M. 464.1, APPROXIMATELY

I. INTRODUCTION

1. Background

In May 2002 the U.S. Army Corps of Engineers (COE), St. Paul and Rock Island Districts, initiated a 10-year effort to re-establish the federally endangered, native freshwater mussel called Higgins eye pearly mussel (Lampsilis higinsii: LH) in the upper Mississippi River System (UMRS). Photographs of adult LHs are shown in Photo 1-1. Such re-establishing efforts are currently being pursued at ten sites in Illinois, Iowa, Minnesota, and Wisconsin. In July 2002, the St. Paul COE District, in cooperation with the inter-agency Mussel Coordination Team (MCT), issued a Relocation Plan, called “Definite Project Report and Environmental Assessment for Relocation Plan for the Endangered Higgins Eye Pearly Mussel (Lampsilis higginsii), Upper Mississippi River and Tributaries, Minnesota, Wisconsin, Iowa, and Illinois.” The recent decline in the LH population has been partly attributable to zebra mussels (Dreissena polymorpha), and the MCT routinely conduct zebra-mussel cleaning operations along the UMRS. LH mussels infected by zebra mussels are shown in Photo 1-2(a) and those cleaned in Photo 1-2(b).

As part of the MCT’s 2007 LH relocation plan, Lucille A. Carver Mississippi Riverside Environmental Research Station (LACMRERS), The University of Iowa, and the IADNR Fairport Fisheries Management recommended that juvenile LH mussels be released in the Mississippi River near Fairport, Iowa in Pool 16. Such a proposed action must fulfill regulatory requirements stipulated under the provisions of the federal Endangered Species Act (ESA) of 1973, as amended, and associated regulations. Section 7(a)(2) of the ESA requires federal agencies to insure that actions authorized, funded, or carried out by them are not likely to jeopardize the continued existence of endangered or threatened species. Juvenile LH mussels have been raised in cages in Lake Pepin in Pool 4 of the Mississippi River by the U.S. Fish & Wildlife Service (see Photo 1-3).

2. Study Objectives

As part of the Environmental Assessment (EA) for a relocation plan for the endangered Higgins eye pearly mussels (LH), it was necessary to conduct a field survey characterizing the mussel habitat surrounding the proposed release site and assessing diversity in mussel species.

(a) (b)

(c) (d) Photo 1-1 Male and female Higgins eye (Lampsilis higginsii) pearly mussels: male’s exterior (a), male’s pearly interior (b), female’s exterior (c), and female’s interior (d)

3. Mussel Survey Site

The general survey area is shown in Figure 1-1, and a detailed survey map is shown in Figure 1-2. In this reach of the Mississippi River, the river flows from northeast to southwest. The rectangular mussel survey area was approximately 800-m long and 160- m wide (total area: 128,000 m2), including six sections, as identified in Figure 1-2. Detailed survey locations and flow depths measured are shown in Figure 1-3, and their GPS coordinates are listed in Table 1-1. It should be noted that an unnamed small creek meets the Mississippi River just upstream from the IADNR Fish Hatchery pond, as shown in Figures 1-2 and 1-3, and Photo 1-4. Bed materials surrounding the creek mouth are primarily coarse sand and gravel, providing excellent freshwater habitats. It should be noted that a small deltaic island exists off the creek mouth (see Photo 1-4). During the mussel surveys, the river stage was stable at EL 544.85 ft (166.07 m) above MSL 1912.

(a) (b)

Photo 1-2 Higgins eye (Lampsilis higginsii) pearly mussels infested by zebra mussels (Dreissena polymorpha) (a), and those zebra-free LHs after removing zebra mussels – photos were taken on 1 August 2007 during MCT’s mussel cleaning effort near Cordova, IL in the Mississippi River

(a) (b)

Photo 1-3 Juvenile Higgins eye (Lampsilis higginsii) pearly mussels raised in a cage in Lake Pepin near Lake City, Minnesota by the U.S. Fish & Wildlife Service (a), and a close-up view of 2-yr old Higgins eye mussels (b) – notice some zebra mussels attached to Higgins eye mussels

3 Survey Area

Figure 1-1 General mussel survey area near Fairport, Iowa (U.S.G.S. topographic map powered by TOPO! published by National Geographic)

4 t or np ve Da To

Highway 22 Q1 IA DNR Fairport Muskrat D14 Fish Hatchery Ponds Midden D15 e tin Q3 D13 Sec 6 usca Q2 o M T D10 Q4 D12 Sec 5 Creek Q5 D11 Mouth D7 D8 Sec 4 P1 D9 P2 W Survey Area: ~ 800 m by D3 O 160 m = 128,000 sq m D1 Sec 3 FL D2

D6 Sec 2 D5 D4 0 100 200 Sec 1 Note: P - Pollywog; D - Dive; and Q - Quadrats Scale in meters

Figure 1-2 A close-up map showing mussel data collection sites near Fairport, Iowa (2000 color infrared digital orthophoto shown was downloaded from the Iowa State University’s Iowa Geographic Map Server site: http://komodo.gis.iastate.edu/)

Creek Highway 22

Muskrat Q1 (8.3 ft) Midden D14 (8.5 ft) IA DNR Fairport D15 (8.6 ft) Fish Hatchery Ponds Q3 (9.7 ft) D13 (10.0 ft) Q2 (9.4 ft) D10 (9.6 ft) D12 (11.3 ft) Q4 (11.8 ft) D7 (9.3 ft) Q5 (9.5 ft) Creek Mouth D11 (11.7 ft) D8 (10.5 ft)

P2 P1 D3 D9 (13.2 ft) D1 D2 FLOW

D6 (12.8 ft)

D4 (16.0 ft) 0 100 200 D5 (15.3 ft) Scale in meters Note: P - Pollywog; D - Dive; and Q - Quadrats (no depth recorded at D1, D2, & D3)

Figure 1-3 A close-up map identifying mussel data collection sites with local flow depths near Fairport, Iowa (2000 color infrared digital orthophoto shown was downloaded from the Iowa State University’s Iowa Geographic Map Server site: http://komodo.gis.iastate.edu/)

5 LACMRERS Mussel Survey: Data Collection Sites Mississippi River Fairport Site near IADNR Fairport Fish Hatchery - Approximately, RM 463.5~464.1, Pool 16 (9/12/06~9/13/06) Site Name UTM Datum UTM Zone Easting Northing Depth (ft) Pollywog 1 27 15 676074E 4589311N < 2 ft Pollywog 2 27 15 676068E 4589298N < 2 ft Dive 1 27 15 676063E 4589286N No record Dive 2 27 15 676089E 4589249N No record Dive 3 27 15 676080E 4589279N No record Dive 4 27 15 675997E 4589124N 16.0 Dive 5 27 15 675961E 4589122N 15.3 Dive 6 27 15 675974E 4589183N 12.8 Dive 7 27 15 676191E 4589397N 9.3 Dive 8 27 15 676209E 4589372N 10.5 Dive 9 27 15 676245E 4589311N 13.2 Dive 10 27 15 676301E 4589473N 9.6 Dive 11 27 15 676348E 4589415N 11.7 Dive 12 27 15 676441E 4589463N 11.3 Dive 13 27 15 676413E 4589523N 10.0 Dive 14 27 15 676542E 4589650N 8.5 Dive 15 27 15 676566E 4589591N 8.6 Quadrat 1 27 15 676567E 4589650N 8.3 Quadrat 2 27 15 676324E 4589471N 9.4 Quadrat 3 27 15 676400E 4589532N 9.7 Quadrat 4 27 15 676343E 4589456N 11.8 Quadrat 5 27 15 676218E 4589403N 9.5

Table 1-1 GPS locations and flow depths at individual data sampling sites

Photo 1-4 The mouth of an unnamed creek (top center) – flow direction from right to left

6 4. Survey Methods

Prior to the mussel surveys a brailing operation was conducted to determine survey boundaries as well as to identify tree snags (see Photo 1-5). Freshwater mussel surveys were conducted primarily by diving with a few pollywogging operations and a muskrat midden survey. There were two pollywogging sites, identified as P1 and P2 in Figures 1- 2 and 1-3; fifteen dives, typically 5-min. dives, identified as D1 through D15 in Figures 1-2 and 1-3; and five quadrat sites, identified as Q1 through Q5 in Figures 1-2 and 1-3. Live mussel samples collected were identified for species, and their lengths and ages were determined (see Photo 1-6). Furthermore, live zebra mussels attached to live native mussels were counted and their sizes were also recorded.

Photo 1-5 Brailing operation prior to mussel surveys

Photo 1-6 Species identifications for a quadrat sample

7 II. PRESENTATION OF INDIVIDUAL SURVEY RESULTS

1. Pollywog 1 (UTM: NAD 27 and Zone 15 - 676074E/4589311N)

This site, shown as P1 in Figure 1-2, was located just upstream from a small deltaic island formed at the mouth of a small unnamed creek adjacent to the east side of IADNR’s Fairport Fish Hatchery ponds. A 10-min. long pollywog yielded a collection of nine live mussels, as shown in Photo 2-1. Five species of mussels were identified, including Giant floater, Mapleleaf, Mucket, Plain pocketbook, and Threeridge. Their sizes and ages were also recorded and they are shown in Table 2-1. The mussel age varied from 4 to 7 years, and the substrate consisted of sand and gravel. No live zebra mussels were found on these mussels.

Photo 2-1 Nine live mussels collected in Pollywog 1

8 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 1:40:00 PM (10--min. pollywog) Jon Christensen Personnel Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: depth < 2 ft UTM Coordinates NAD 27 15 676074E 4589311N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Pollywog 1 YES for mussel group Bed Material Sand/Gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1(mm) L #1 Yrs #2(mm) L #2 Yrs #3(MM) L #3 Yrs #4(mm) L #4 Yrs #5(mm) L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER 1 128 5 Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 75 7 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET 2 112 7 103 6 Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK 2 123 6 113 7 Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 3 63 4 59 4 58 4 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL(Live Mussels) 9 SPECIES 5

Table 2-1 Size and age distributions of live mussels collected during Pollywog 1

2. Pollywog 2 (UTM: NAD 27 and Zone 15 - 676068E/4589298N)

This site, shown as P2 in Figure 1-2, was located next to the Pollywog 1 site. A 10-min. long pollywog yielded a collection of five live mussels, as shown in Photo 2-2. Two species of mussels were identified, including Plain pocketbook and Threeridge. Their

9 sizes and ages are shown in Table 2-2. Their ages ranged from 4 to 7 years. No live zebra mussels were found on these mussels. The substrate consisted of sand and gravel.

Photo 2-2 Five live mussels collected in Pollywog 2

3. Dive 1 (UTM: NAD 27 and Zone 15 - 676063E/4589286N)

This site, shown as D1 in Figure 1-2, was located next to Pollywog 1 and Pollywog 2. A five min. dive yielded a collection of seven live mussels of five species, including Mucket, Wabash pigtoe, Plain pocketbook, Threehorn wartyback, and Washboard, as listed in Table 2-3. The mussels collected are shown in Photo 2-3 and a close-up photo of the Mucket (132-mm long and 7-years old) is shown in Photo 2-4. Five 2-3 mm long zebra mussels were attached to the Mucket; four 2-3 mm long zebra mussels to the 8-year old Plain pocketbook; and one 3-mm long zebra to the Washboard. The other two mussels had no zebras attached. The age of these mussels varied from 4 to 14 years.

10 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 1:40:00 PM (10--min. pollywog) Eric Giebelstein Personnel Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: depth < 2 ft UTM Coordinates NAD 27 15 676068E 4589298N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Pollywog 2 YES for mussel group Bed Material Sand/Gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK 1 112 7 Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 4 854605584495 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL(Live Mussels) 5 SPECIES 2

Table 2-2 Size and age distributions of live mussels collected during Pollywog 2

Photo 2-3 Seven live mussels collected in Dive 1

Photo 2-4 Mucket (Actinonaias ligamentina) collected in Dive 1 (anterior: right and posterior: left)

12 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 1:35:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = not recorded UTM Coordinates NAD 27 15 676063E 4589286N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 1 YES for mussel group Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET 1 132 7 Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 2 66 7 50 4 Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK 2 120 14 120 8 Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 48 7 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 140 10 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL(Live Mussels) 7 SPECIES 5

Table 2-3 Size and age distributions of live mussels collected during Dive 1

13 4. Dive 2 (UTM: NAD 27 and Zone 15 - 676089E/4589249N)

This site, shown as D2 in Figure 1-2, was located approximately 45 m south south-east from Dive 1 toward the main channel. Two 5-min. dives yielded a collection of ten live mussels of five species, including Mapleleaf, Monkeyface, Pimpleback, Plain pocketbook, and Washboard, as listed in Table 2-4. The mussels collected are shown in Photo 2-5. Two 2-3 mm long zebra mussels were attached to the 6-year old Monkeyface. The other nine mussels had no zebras attached. The age of the sampled mussels varied from 4 to 8 years. The substrate material was not recorded at this site.

Photo 2-5 Ten live mussels collected in Dive 2

14 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 1:30:00 PM (two 5-min. dives) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = not recorded UTM Coordinates NAD 27 15 676089E 4589249N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 2 YES for mussel group Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 70 5 Quadrula metanevra MONKEYFACE T 2 70 8 70 6 Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 2 69 7 60 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK 1 97 6 Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 4 142 7 115 7 108 6 95 4 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 10 SPECIES 5

Table 2-4 Size and age distributions of live mussels collected during Dive 2

15 5. Dive 3 (UTM: NAD 27 and Zone 15 - 6760809E/4589279N)

This site, shown as D3 in Figure 1-2, was located approximately 25 m south east from Dive 1 between Dive 1 and Dive 2. A 5-min. dive yielded a collection of fourteen live mussels of eight species, including Black sandshell, Butterfly, Hickorynut, Mapleleaf, Pimpleback, Threeridge, Wartyback, and Washboard, as listed in Table 2-5. The mussels collected are shown in Photo 2-6. Three 3-mm long zebras were attached to the 123-mm long Washboard; three 3-mm long zebras to the 145-mm long Washboard; two 3-mm long zebras to the 125-mm long Washboard; and three 3-mm long zebras to the 100-mm long Threeridge. The other ten mussels had no zebras attached. The age of the sampled mussels varied from 4 to 7 years. The substrate material was not recorded at this site.

Photo 2-6 Fourteen live mussels collected in Dive 3

16 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 2:17:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = not recorded UTM Coordinates NAD 27 15 676080E 4589279N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 3 YES for mussel group Bed Material sand/gravel/cobble STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T 1 138 6 Ellipsaria lineolata BUTTERFLY ETT 2 80 4 75 4 Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 2 59 6 52 5 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 63 5 78 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 55 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 3 100 6 95 5 99 5 Quadrula nodulata WARTYBACK T 1 60 7 Megalonaias nervosa WASHBOARD 3 123 6 145 7 125 6 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 14 SPECIES 8

Table 2-5 Size and age distributions of live mussels collected during Dive 3

17 6. Dive 4 (UTM: NAD 27 and Zone 15 - 675997E/4589124N)

This site, shown as D4 in Figure 1-2, was located approximately 175 m south south-east from Dive 1 where flow depth was 16.0 ft. A 5-min. dive yielded a collection of nine live mussels of five species, including Hickorynut, Monkeyface, Pimpleback, Threeridge, and Washboard, as listed in Table 2-6. The mussels collected are shown in Photo 2-7. No live zebras were found attached to any live mussels sampled at this site. The age of the sampled mussels varied from 2 (Pimpleback) to 8 years (Washboard). The substrate material comprised sand, gravel, and cobble.

Photo 2-7 Nine live mussels collected in Dive 4

18 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 2:45:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d =16.0 ft UTM Coordinates NAD 27 15 675997E 4589124N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 4 YES for mussel group Bed Material sand/gravel/cobble STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 50 5 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T 2 70 5 62 6 Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 33 2 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 1 31 4 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 4 128 8 115 6 126 7 112 8 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 9 SPECIES 5

Table 2-6 Size and age distributions of live mussels collected during Dive 4

19 7. Dive 5 (UTM: NAD 27 and Zone 15 - 6759617E/4589122N)

This site, shown as D5 in Figure 1-2, was located approximately 36 m west from Dive 4 where flow depth was 15.3 ft. A 5-min. dive yielded a collection of only two live mussels of two species, including Mapleleaf and Threeridge, as listed in Table 2-7. The mussels collected are shown in Photo 2-8. Two 3-mm long zebras were found attached to the Mapleleaf. The age of the Mapleleaf was 8 years, and that of the Threeridge was 3 years. The substrate material was not recorded at this site.

Photo 2-8 Two live mussels collected in Dive 5

20 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 3:01:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 15.3 ft UTM Coordinates NAD 27 15 675961E 4589122N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 5 YES for mussel group Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 70 8 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 1 38 3 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 2 SPECIES 2

Table 2-7 Size and age distributions of live mussels collected during Dive 5

21 8. Dive 6 (UTM: NAD 27 and Zone 15 - 675974E/4589183N)

This site, shown as D6 in Figure 1-2, was located approximately 62 m north from Dive 5 where flow depth was 12.8 ft. A 5-min. dive yielded a collection of ten live mussels of six species, including Black sandshell, Butterfly, Hickorynut, Mapleleaf, Pimpleback, and Threehorn wartyback, as listed in Table 2-8. The mussels collected are shown in Photo 2-9. One 3-mm long zebra was found attached to the Black sandshell; one 3-mm long zebra to the 8-year old Mapleleaf; and one 3-mm long zebra to the 41-mm long Threehorn watryback. The age of these mussels ranged from 3 to 8 years. Threehorn watrybacks were very young (one 4-years old and three 3-years old). The substrate material was silt and clay.

Photo 2-9 Ten live mussels collected in Dive 6

22 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 3:12:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 12.8 ft UTM Coordinates NAD 27 15 675974E 4589183N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 6 YES for mussel group Bed Material silt/clay STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T 1 130 8 Ellipsaria lineolata BUTTERFLY ETT 1 75 4 Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 52 5 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 2 84 8 75 6 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 50 5 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 4 504503413353 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 10 SPECIES 6

Table 2-8 Size and age distributions of live mussels collected during Dive 6

23 9. Dive 7 (UTM: NAD 27 and Zone 15 - 676191E/4589397N)

This site, shown as D7 in Figure 1-2, was located approximately 169 m north east from Dive 1 near the Iowa shore where flow depth was 9.3 ft. A 5-min. dive yielded a collection of eleven live mussels of eight species, including Hickorynut, Mapleleaf, Mucket, Squawfoot, Threehorn wartyback, Threeridge, Wabash pigtoe, and Washboard, as listed in Table 2-9. The mussels collected are shown in Photo 2-10. Close-up views of Mucket exteriors are shown in Photos 2-11 and 2-12. One 3-mm long zebra was found attached to the 5-year old Threeridge; and two 3-mm long zebras to the 8-year old Threehorn watryback, and one 3-mm long zebra to the Squawfoot. The age of these mussels ranged from 3 to 9 years. The substrate material was sand and gravel. This could be an excellent candidate site for Higgins eye relocations because of the excellent substrate and the diversity in species.

Photo 2-10 Eleven live mussels collected in Dive 7

Photo 2-11 Mucket (Actinonaias ligamentina) collected in Dive 7 (anterior: right and posterior: left)

Photo 2-12 Mucket (Actinonaias ligamentina) collected in Dive 7 (anterior: left and posterior: right)

25 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 3:28:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.3 ft UTM Coordinates NAD 27 15 676191E 4589397N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 7 YES for mussel group Bed Material sand/gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 60 4 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 55 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET 1 110 7 Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 1 65 9 Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 50 5 80 8 Amblema plicata THREERIDGE 3 77 6 77 5 40 3 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 144 9 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T 1 92 5 TOTAL (Live Mussels) 11 SPECIES 8

Table 2-9 Size and age distributions of live mussels collected during Dive 7

26 10. Dive 8 (UTM: NAD 27 and Zone 15 - 676209E/4589372N)

This site, shown as D8 in Figure 1-2, was located approximately 30 m south east from Dive 7 where flow depth was 10.5 ft. A 5-min. dive yielded a collection of eighteen live mussels of seven species, including Mapleleaf, Monkeyface, Pimpleback, Threehorn wartyback, Threeridge, Wartyback, and Washboard, as listed in Table 2-10. The mussels collected are shown in Photo 2-13. Four 3-mm long zebras were found attached to the 142-mm long Washboard; three 3-mm long zebras to the 132-mm long Washboard; ten 3-mm long zebras to the 148-mm Washboard; one 3-mm long zebra to the 81-mm long Mapleleaf; one 3-mm long zebra to the 74-mm long Mapleleaf; one 3-mm long zebra to the 67-mm Threeridge; and one 3-mm long zebra to the 35-mm Threeridge. It should be noted that more zebras were found in elder mussels, particularly in Washboard. The age of these mussels ranged from 3 to 9 years. The substrate material was sand and silt. This could also be an excellent candidate site for Higgins eye relocations because of the excellent substrate and the diversity in species.

Photo 2-13 Eighteen live mussels collected in Dive 8

27 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 3:39:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Giebelstein, Personnel Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 10.5 ft UTM Coordinates NAD 27 15 676209E 4589372N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 8 YES for mussel group Bed Material sand/silt STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs #6 L (mm) #6 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 3 81 8 80 9 74 8 Quadrula metanevra MONKEYFACE T 1 40 4 Actinonaias ligamentina MUCKET Utterback ia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 45 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 3 52 4 40 4 43 5 Amblema plicata THREERIDGE 6 856805867676424353 Quadrula nodulata WARTYBACK T 1 50 5 Megalonaias nervosa WASHBOARD 3 142 8 132 9 148 9 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 18 SPECIES 7

Table 2-10 Size and age distributions of live mussels collected during Dive 8

28 11. Dive 9 (UTM: NAD 27 and Zone 15 - 676245E/4589311N)

This site, shown as D9 in Figure 1-2, was located approximately 71 m south east from Dive 8 where flow depth was 13.2 ft. A 5-min. dive yielded a collection of only two live mussels of one species which was Threeridge, as listed in Table 2-11. The mussels collected are shown in Photo 2-14. Three 3-mm long zebras were found attached to the 81-mm long Threeridge. The ages of these mussels were 4 and 9 years, respectively. The substrate material was sand and gravel. It should be noted that despite the excellent substrate material only a few live mussels were found at this site. It could be due to large flow depth (13.2 ft) at this site as compared to Dive 7 (depth: 9.3 ft) and Dive 8 (depth: 10.5 ft).

Photo 2-14 Two live mussels collected in Dive 9

29 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 12-Sep-06 Time 3:52:00 PM (5-min. dive) Bob Schanzle, Rich Lewis, Ed Walsh, Bernard Schonhoff, Jon Christensen, Eric Personnel Giebelstein, Steve Johnson, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 13.2 ft UTM Coordinates NAD 27 15 676245E 4589311N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 9 YES for mussel group Bed Material sand/gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm)#1 L #1 Yrs (mm)#2 L #2 Yrs (MM)#3 L #3 Yrs (mm)#4 L #4 Yrs (mm)#5 L #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 2 81 9 42 4 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 2 SPECIES 1

Table 2-11 Size and age distributions of live mussels collected during Dive 9

30 12. Dive 10 (UTM: NAD 27 and Zone 15 - 676301E/4589473N)

This site, shown as D10 in Figure 1-2, was located approximately 134 m north east from Dive 7 where flow depth was 9.6 ft. A 5-min. dive yielded a collection of six live mussels of four species, including Wabash pigtoe, Pimpleback, Threehorn wartyback, and Washboard, as listed in Table 2-12. The mussels collected are shown in Photo 2-15. Five 3-mm long zebras were found attached to the 136-mm long Washboard and none was found in the rest of the mussels. The age of these mussels ranged from 4 to 9 years. The two Washboard mussels were both 9-years old. The substrate material was sand and gravel.

Photo 2-15 Six live mussels collected in Dive 10

31 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 10:30 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d =9.6 ft UTM Coordinates NAD 27 15 676301E 4589473N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 10 YES for mussel group Bed Material sand/gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 1 33 4 Quadrula pustulosa PIMPLEBACK 1 70 7 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 48 4 40 4 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 2 136 9 134 9 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 6 SPECIES 4

Table 2-12 Size and age distributions of live mussels collected during Dive 10

32 13. Dive 11 (UTM: NAD 27 and Zone 15 - 676348E/4589415N)

This site, shown as D11 in Figure 1-2, was located approximately 75 m south east from Dive 10 where flow depth was 11.7 ft. A 5-min. dive yielded a collection of eight live mussels of four species, including Mapleleaf, Wabash pigtoe, Pimpleback, and Threeridge, as listed in Table 2-13. The mussels collected are shown in Photo 2-16. Three 5-mm long zebras were found attached to the 58-mm long Pimpleback; one 5-mm long zebra to the 28-mm long Pimpleback; two 3-mm long zebras to the Mapleleaf; one 4-mm long zebra to the 38-mm long Threeridge; one 2-mm-long zebra to the 24-mm long Threeridge; and two 3-mm long zebras to the 17-mm long Threeridge. The age of these mussels ranged from 2 to 5 years. In particular, the Threeridge mussels were juveniles (two were 2-years old and the other two were 3-years and 4-years old). The substrate material was sand and gravel.

Photo 2-16 Eight live mussels collected in Dive 11

33 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 10:43 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 11.7 ft UTM Coordinates NAD 27 15 676348E 4589415N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 11 YES for mussel group Bed Material sand/gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 41 4 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 1 20 2 Quadrula pustulosa PIMPLEBACK 2 58 5 28 3 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 4 384243222172 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 8 SPECIES 4

Table 2-13 Size and age distributions of live mussels collected during Dive 11

34 14. Dive 12 (UTM: NAD 27 and Zone 15 - 676441E/4589463N)

This site, shown as D12 in Figure 1-2, was located approximately 105 m north east from Dive 11 where flow depth was 11.3 ft. A 5-min. dive yielded a collection of six live mussels of three species, including Hickorynut, Pimpleback, and Threehorn wartyback, as listed in Table 2-14. The mussels collected are shown in Photo 2-17. Two 4-mm long zebras were found attached to the 18-mm long Pimpleback; one 4-mm long zebra to the 24-mm long Hickorynut; and two 3-mm long zebras to the 31-mm long Hickorynut. The age of these mussels ranged from 2 to 6 years. The Pimpleback was 2-years old and 18- mm long. The substrate material was sand.

Photo 2-17 Six live mussels collected in Dive 12

35 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 11:05 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d =11.3 ft UTM Coordinates NAD 27 15 676441E 4589463N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 12 YES for mussel group Bed Material sand STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 2 31 4 24 3 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 18 2 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 3 52 6 40 3 34 3 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 6 SPECIES 3

Table 2-14 Size and age distributions of live mussels collected during Dive 12

36 15. Dive 13 (UTM: NAD 27 and Zone 15 - 676413E/4589523N)

This site, shown as D13 in Figure 1-2, was located approximately 66 m north west from Dive 12 where flow depth was 10.0 ft. A 5-min. dive yielded a collection of thirteen live mussels of nine species, including Butterfly, Hickorynut, Mapleleaf, Mucket, Pimpleback, Threehorn wartyback, Threeridge, Wartyback, and Washboard, as listed in Table 2-15. The mussels collected are shown in Photo 2-18. Eleven 3~4-mm long zebras were found attached to the 138-mm long Washboard; one 4-mm long zebra to the 65-mm long Mapleleaf; two 4-mm long zebras to the 48-mm long Pimpleback; and one 5-mm long zebra to the 61-mm long Pimpleback. The age of these mussels ranged from 3 to 7 years. The substrate material was sand.

Photo 2-18 Thirteen live mussels collected in Dive 13

37 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 11:17 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 10.0 ft UTM Coordinates NAD 27 15 676413E 4589523N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 13 YES for mussel group Bed Material sand/gravel STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT 1 50 4 Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 56 5 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 2 65 6 68 7 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET 1 102 7 Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 2 48 5 61 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 52 4 39 4 Amblema plicata THREERIDGE 2 42 4 43 3 Quadrula nodulata WARTYBACK T 1 38 6 Megalonaias nervosa WASHBOARD 1 138 7 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 13 SPECIES 9

Table 2-15 Size and age distributions of live mussels collected during Dive 13

38 16. Dive 14 (UTM: NAD 27 and Zone 15 - 676542E/4589650N)

This site, shown as D14 in Figure 1-2, was located approximately 181 m north east from Dive 13 where flow depth was 8.5 ft. This site was very close to the Iowa shoreline. A 5-min. dive yielded a collection of sixteen live mussels of seven species, including, Hickorynut, Monkeyface, Wabash pigtoe, Pimpleback, Threehorn wartyback, Threeridge, and Washboard, as listed in Table 2-16. The mussels collected are shown in Photo 2-19. Zebra mussels were found to be attached only to the Washboard at this site - six 3~4-mm long zebras were attached to the 140-mm long Washboard; five 3~4-mm long zebras to the 145-mm long Washboard; five 3~4-mm long zebras to the 139-mm long Washboard; and seven 3~4-mm long zebras to the 127-mm long Washboard. The age of these mussels ranged from 2 to 8 years. The ages of those five Pimpleback mussels were 2, 3, 6, 7, and 8 years. All the four Washboard mussels were 8 years old. The substrate material was sandy silt.

Photo 2-19 Sixteen live mussels collected in Dive 14

39 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 11:25 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d =8.5 ft UTM Coordinates NAD 27 15 676542E 4589650N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 14 YES for mussel group Bed Material sandy silt STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 39 4 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T 1 70 8 Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 3 70 5 65 7 54 7 Quadrula pustulosa PIMPLEBACK 5 607516518252303 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 43 4 Amblema plicata THREERIDGE 1 23 3 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 4 140 8 145 8 139 8 127 8 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 16 SPECIES 7

Table 2-16 Size and age distributions of live mussels collected during Dive 14

40 17. Dive 15 (UTM: NAD 27 and Zone 15 - 676566E/4589591N)

This site, shown as D15 in Figure 1-2, was located approximately 64 m south east from Dive 14 where flow depth was 8.6 ft. A 5-min. dive yielded a collection of five live mussels of three species, including, Pimpleback, Threeridge, and Washboard, as listed in Table 2-17. The mussels collected are shown in Photo 2-20. One 3-mm long zebra mussel was found attached to the 50-mm Pimpleback. The age of these mussels ranged from 3 to 7 years. The substrate material was sand.

Photo 2-20 Five live mussels collected in Dive 15

41 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 11:50 A.M. (5-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Melanie Alexander, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 8.6 ft UTM Coordinates NAD 27 15 676566E 4589591N Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Dive 15 YES for mussel group Bed Material sand STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #2 L (mm) #2 Yrs #3 L (MM) #3 Yrs #4 L (mm) #4 Yrs #5 L (mm) #5 Yrs Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 2 50 6 59 7 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 2 74 6 41 3 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 82 7 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 5 SPECIES 3

Table 2-17 Size and age distributions of live mussels collected during Dive 15

42 During the mussel surveys, five quadrat samples (Q1 through Q5) were collected by divers using 0.5-m by 0.5-m square samplers in order to evaluate both the live mussel population density and the density of the zebra mussel population in the substrate. Each sample was spread evenly and divided into four quadrants. Individual live mussels in each quadrant were identified and any zebra mussels attached were recorded. Furthermore, dead mussel shells in each quadrant were classified as “recently dead: RD” and “weathered dead: WD.” Lengths of live zebra mussels that were not attached to live freshwater mussels were also measured.

18. Quadrat 1-A (UTM: NAD 27 and Zone 15 - 676567E/4589650N)

This site, shown as Q1 in Figure 1-2, was located near Sec 6, where flow depth was 8.3 ft. A quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 1-A was the first quadrant sample which had neither live mussels nor dead shells, as shown in Table 2-18. The substrate material was not recorded.

19. Quadrat 1-B (UTM: NAD 27 and Zone 15 - 676567E/4589650N)

This site, shown as Q1 in Figure 1-2, was located near Sec 6, where flow depth was 8.3 ft. A quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 1-B was the second quadrant sample which had three live mussels, including Mapleleaf and Threehorn wartyback mussels, as listed in Table 2-19. No live zebra mussels were found attached to the live mussels. There was one Fragile papershell (half shell) found in this sample. The substrate material was not recorded.

20. Quadrat 1-C (UTM: NAD 27 and Zone 15 - 676567E/4589650N)

This site, shown as Q1 in Figure 1-2, was located near Sec 6, where flow depth was 8.3 ft. A quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 1-C was the third quadrant sample which had two live mussels, including Mapleleaf and Monkeyface mussels, as listed in Table 2-20. One 3-mm long live zebra mussel was found attached to the 56-mm long Mapleleaf. There were four dead shells, including a full Washboard (132-mm long and 8-years old), one Fragile papershell (half shell, 110-mm long), one Pimpleback (2-years old and 61-mm long: RD), and Fragile papershell (half shell, 60-mm long: WD). The substrate material was not recorded.

43 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 8.3 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676567E 4589650N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 1-A No Photos Bed Material Not recoded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 0 SPECIES 0 Note: No live Zebs were found

Table 2-18 Size and age distributions of live mussels collected in Quadrat 1-A

44 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 8.3 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676567E 4589650N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 1-B No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 2 63 7 54 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 50 5 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 3 SPECIES 2 Note: No live Zebs were found

Table 2-19 Size and age distributions of live mussels collected in Quadrat 1-B

45 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 8.3 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676567E 4589650N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 1-C No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 56 5 Quadrula metanevra MONKEYFACE T 1 68 9 Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 2 SPECIES 2 Note: No live Zebs were found

Table 2-20 Size and age distributions of live mussels collected in Quadrat 1-C

46 21. Quadrat 1-D (UTM: NAD 27 and Zone 15 - 676567E/4589650N)

This site, shown as Q1 in Figure 1-2, was located near Sec 6, where flow depth was 8.3 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 1-D was the forth quadrant sample which had three live mussels, including Hickorynut and Mapleleaf mussels, as listed in Table 2-21. There was no live zebra mussel attached to the live mussels. However, Quadrat 1-D sample found four live zebra mussels whose sizes were 5-mm, 5-mm, 13-mm, and 13-mm long. There were two dead shells, including a full Hickorynut (4-years old and 56-mm long: RD) and a full Mapleleaf (4-years old and 45-mm long: RD). The substrate material was not recorded.

22. Quadrat 2-A (UTM: NAD 27 and Zone 15 - 676324E/4589471N)

This site, shown as Q2 in Figure 1-2, was located near Sec 4, where flow depth was 9.4 ft. A Quadrat sample was obtained by an 18-min. dive which was divided into four quadrants. Quadrat 2-A was the first quadrant sample which had four live mussels, including Hickorynut, Pimpleback, and Threehorn wartyback mussels, as listed in Table 2-22. There was no live zebra mussel attached to the live mussels. There were eight dead shells, including Butterfly, Deertoe, Fragile papershell, Hickorynut, Threehorn wartyback, Wabash pigtoe, Wartyback, and Washboard. Neither their lengths nor ages were recorded. The substrate material was not recorded.

23. Quadrat 2-B (UTM: NAD 27 and Zone 15 - 676324E/4589471N)

This site, shown as Q2 in Figure 1-2, was located near Sec 4, where flow depth was 9.4 ft. A Quadrat sample was obtained by an 18-min. dive which was divided into four quadrants. Quadrat 2-B was the second quadrant sample which had seven live mussels, including Mucket, Wabash pigtoe, Pimpleback, Threehorn wartyback, and Threeridge mussels, as listed in Table 2-23. There was no live zebra mussel attached to the live mussels; however, there was one live zebra mussel found in this quadrant which was 18- mm long. There were eight dead shells, including Butterfly, Black sandshell, Hickorynut, Mapleleaf, Pimpleback, Pink heelsplitter, Washboard, and White heelsplitter. Neither their lengths nor ages were recorded. The substrate material was not recorded.

47 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 8.3 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676567E 4589650N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 1-D No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 35 3 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 2 68 6 66 7 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 3 SPECIES 2 Note: Four live Zebs were found -- 13 mm/13 mm/8 mm/5 mm

Table 2-21 Size and age distributions of live mussels collected in Quadrat 1-D

48 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:47 P.M. (18-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.4 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676324E 4589471N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 2-A No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 60 3 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 30 4 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 40 3 35 2 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 4 SPECIES 3 Note: No live Zebs were found

Table 2-22 Size and age distributions of live mussels collected in Quadrat 2-A

49 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:47 P.M. (18-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.4 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676324E 4589471N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 2-B No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET 1 113 7 Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 2 70 8 58 6 Quadrula pustulosa PIMPLEBACK 2 66 6 46 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 46 4 Amblema plicata THREERIDGE 1 78 7 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 7 SPECIES 5 Note: No live Zebs were found

Table 2-23 Size and age distributions of live mussels collected in Quadrat 2-B

50 24. Quadrat 2-C (UTM: NAD 27 and Zone 15 - 676324E/4589471N)

This site, shown as Q2 in Figure 1-2, was located near Sec 4, where flow depth was 9.4 ft. A Quadrat sample was obtained by an 18-min. dive which was divided into four quadrants. Quadrat 2-D was the third quadrant sample which had ten live mussels, including Pimpleback, Threehorn wartyback, and Threeridge mussels, as listed in Table 2-24. One 3-mm long zebra mussel was found attached to the 40-mm long Pimpleback; and one 3-mm long zebra to the 31-mm long Threehorn wartyback. There were eight dead mussel species, including Butterfly, Black sandshell, Hickorynut, Mapleleaf, Monkeyface, Threeridge, Wartyback, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

25. Quadrat 2-D (UTM: NAD 27 and Zone 15 - 676324E/4589471N)

This site, shown as Q2 in Figure 1-2, was located near Sec 4, where flow depth was 9.4 ft. A Quadrat sample was obtained by an 18-min. dive which was divided into four quadrants. Quadrat 2-D was the forth quadrant sample which had five live mussels, including Wabash pigtoe, Pimpleback, Threehorn wartyback, and Washboard mussels, as listed in Table 2-25. Two 3-mm long zebra mussels were found attached to the 45-mm long Threehorn wartyback. There were eight dead mussel species, including Butterfly, Black sandshell, Hickorynut, Wabash pigtoe, Pimpleback, Threeridge, Pink heelsplitter, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

26. Quadrat 3-A (UTM: NAD 27 and Zone 15 - 676400E/4589532N)

This site, shown as Q3 in Figure 1-2, was located along Sec 5, where flow depth was 9.7 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 3-A was the first quadrant sample which had seven live mussels, including Mapleleaf, Pimpleback, and Threeridge, as listed in Table 2-26. Three 5-mm long zebra mussels were found attached to the 62-mm long Pimpleback and two 4-mm long zebra mussels attached to the Threeridge. There were nine dead mussel species, including Butterfly, Black sandshell, Hickorynut, Wabash pigtoe, Pimpleback, Three horn wartyback, Threeridge, Pink heelsplitter, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material consisted of sand and gravels with some silt on top.

51 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:47 P.M. (18-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.4 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676324E 4589471N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 2-C No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 4 565526526405 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 3 39 3 55 5 31 2 Amblema plicata THREERIDGE 3 89 6 42 4 45 5 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 10 SPECIES 3 Note: No live Zebs were found

Table 2-24 Size and age distributions of live mussels collected in Quadrat 2-C

52 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 1:47 P.M. (18-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.4 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676324E 4589471N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 2-D No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) #1 Yrs #1 L (mm) #2 Yrs #2 L (MM) #3 Yrs #3 L (mm) #4 Yrs #4 L (mm) #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 1 58 9 Quadrula pustulosa PIMPLEBACK 1 53 6 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 50 6 45 5 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 98 7 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 5 SPECIES 4 Note: No live Zebs were found

Table 2-25 Size and age distributions of live mussels collected in Quadrat 2-D

53 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.7 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676400E 4589532N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 3-A No Photos Bed Material sand/gravel w/silt on top STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 3 74 6 62 7 67 6 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 3 62 7 37 4 27 3 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 1 80 6 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 7 SPECIES 3 Note: No live Zebs were found

Table 2-26 Size and age distributions of live mussels collected in Quadrat 3-A

54 27. Quadrat 3-B (UTM: NAD 27 and Zone 15 - 676400E/4589532N)

This site, shown as Q3 in Figure 1-2, was located along Sec 5, where flow depth was 9.7 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 3-B was the second quadrant sample which had seven live mussels, including Hickorynut, Mapleleaf, Pimpleback, and Threehorn wartyback, as listed in Table 2-27. Four 5-mm long zebra mussels were found attached to the 23-mm long Pimpleback and two 5-mm long zebra mussels attached to the other 23-mm long Pimpleback. There were five dead mussel species, including Fragile papershell, Hickorynut, Giant floater, Threeridge, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material consisted of sand and gravels with some silt on top.

28. Quadrat 3-C (UTM: NAD 27 and Zone 15 - 676400E/4589532N)

This site, shown as Q3 in Figure 1-2, was located along Sec 5, where flow depth was 9.7 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 3-B was the third quadrant sample which had six live mussels, including Mapleleaf, Pimpleback, Threeridge, Wartyback, and Washboard, as listed in Table 2-28. Two 3-mm long zebra mussels were found attached to the 138-mm long Washboard and one 3-mm long zebra mussel attached to the 30-mm long Pimpleback. There were six dead mussel species, including Butterfly, Hickorynut, Giant floater, Threeridge, Washboard, and White heelsplitter, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material consisted of sand and gravels with some silt on top.

29. Quadrat 3-D (UTM: NAD 27 and Zone 15 - 676400E/4589532N)

This site, shown as Q3 in Figure 1-2, was located along Sec 5, where flow depth was 9.7 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 3-D was the forth quadrant sample which had eleven live mussels, including Hickorynut, Mapleleaf, Pimpleback, Threeridge, and Washboard, as listed in Table 2-29. Three 2-mm long zebra mussels were found attached to the 130-mm long Washboard and four 2-mm long zebra mussels attached to the 88-mm long Threeridge. There were six dead mussel species, including Black sandshell, Giant floater, Pink heelsplitter, Rock pocketbook (Arcidens confragosus), Threeridge, and Washboard, which were all noted as WDs except for Black sandshell noted as RD. Neither their lengths nor ages were recorded. The substrate material consisted of sand and gravels with some silt on top.

55 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.7 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676400E 4589532N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 3-B No Photos Bed Material sand/gravel w/silt on top STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 2 60 3 43 4 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 28 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 3 26 4 23 3 23 2 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 18 1 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 7 SPECIES 4 Note: No live Zebs were found

Table 2-27 Size and age distributions of live mussels collected in Quadrat 3-B

56 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.7 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676400E 4589532N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 3-C No Photos Bed Material sand/gravel w/silt on top STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 32 3 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 30 4 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 2 83 9 93 7 Quadrula nodulata WARTYBACK T 1 68 6 Megalonaias nervosa WASHBOARD 1 138 9 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 6 SPECIES 5 Note: No live Zebs were found

Table 2-28 Size and age distributions of live mussels collected in Quadrat 3-C

57 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:25 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.7 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676400E 4589532N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 3-D No Photos Bed Material sand/gravel w/silt on top STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 52 6 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 2 60 4 50 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 3 50 4 35 4 28 4 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE 2 88 5 45 3 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 3 130 7 75 5 93 6 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 11 SPECIES 5 Note: No live Zebs were found

Table 2-29 Size and age distributions of live mussels collected in Quadrat 3-D

58 30. Quadrat 4-A (UTM: NAD 27 and Zone 15 - 676343E/4589456N)

This site, shown as Q4 in Figure 1-2, was located near Sec 5 about 24 m southeast from Quadrat 2, where flow depth was 11.8 ft. A Quadrat sample was obtained by a 10-min. dive which was divided into four quadrants. Quadrat 4-A was the first quadrant sample which had only one live Wabash pigtoe, as shown in Table 2-30, which was not infested by zebra mussels. There were four dead mussel species, including Fragile papershell, Pimpleback, Pink heelsplitter, and Threeridge, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material consisted of sand.

31. Quadrat 4-B (UTM: NAD 27 and Zone 15 - 676343E/4589456N)

This site, shown as Q4 in Figure 1-2, was located near Sec 5 about 24 m southeast from Quadrat 2, where flow depth was 11.8 ft. A Quadrat sample was obtained by a 10-min. dive which was divided into four quadrants. Quadrat 4-B was the second quadrant sample which had no live mussel, as indicated in Table 2-31. There was one dead Pimpleback which was noted as RD. Neither its length nor its age was recorded. The substrate material consisted of sand.

32. Quadrat 4-C (UTM: NAD 27 and Zone 15 - 676343E/4589456N)

This site, shown as Q4 in Figure 1-2, was located near Sec 5 about 24 m southeast from Quadrat 2, where flow depth was 11.8 ft. A Quadrat sample was obtained by a 10-min. dive which was divided into four quadrants. Quadrat 4-C was the third quadrant sample which had three live mussels, including Pimpleback, Plain pocketbook, and Threehorn wartyback, as shown in Table 2-32. Two 3-mm long zebra mussels were found attached to the Threehorn wartyback. There were two dead mussel species, including Pimpleback and Threeridge, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material consisted of sand.

33. Quadrat 4-D (UTM: NAD 27 and Zone 15 - 676343E/4589456N)

This site, shown as Q4 in Figure 1-2, was located near Sec 5 about 24 m southeast from Quadrat 2, where flow depth was 11.8 ft. A Quadrat sample was obtained by a 10-min. dive which was divided into four quadrants. Quadrat 4-D was the fourth quadrant sample which had three live mussels, including Deertoe, Monkeyface, and Threehorn wartyback, as shown in Table 2-33. One 3-mm long zebra mussel was found attached to the Monkeyface. There were no dead mussel species in this sample. The substrate material consisted of sand.

59 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:43 P.M. (10-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 11.8 ft -- 0.25 sq UTM Coordinates NAD 27 15 676343E 4589456N m Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 4-A No Photos Bed Material sand STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH 1 20 2 Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 1 SPECIES 1 Note: No live Zebs were found

Table 2-30 Size and age distributions of live mussels collected in Quadrat 4-A

60 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:43 P.M. (10-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 11.8 ft -- 0.25 sq UTM Coordinates NAD 27 15 676343E 4589456N m Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 4-B No Photos Bed Material sand STATUS #, Length (mm) & Age (yrs)

Table 2-31 Size and age distributions of live mussels collected in Quadrat 4-B

61 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:43 P.M. (10-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 11.8 ft -- 0.25 sq UTM Coordinates NAD 27 15 676343E 4589456N m Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 4-C No Photos Bed Material sand STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 20 2 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK 1 89 4 Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 48 4 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 3 SPECIES 3 Note: No live Zebs were found

Table 2-32 Size and age distributions of live mussels collected in Quadrat 4-C

62 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 2:43 P.M. (10-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 11.8 ft -- 0.25 sq UTM Coordinates NAD 27 15 676343E 4589456N m Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 4-D No Photos Bed Material sand STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE 1 27 2 Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF Quadrula metanevra MONKEYFACE T 1 46 3 Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 1 56 4 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 3 SPECIES 3 Note: No live Zebs were found

Table 2-33 Size and age distributions of live mussels collected in Quadrat 4-D

63 34. Quadrat 5-A (UTM: NAD 27 and Zone 15 - 676218E/4589403N)

This site, shown as Q5 in Figure 1-2, was located near Sec 3, where flow depth was 9.5 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 5-A was the first quadrant sample which had eight live mussels, including Hickorynut, Mapleleaf, Pimpleback, Threehorn wartyback, and Threeridge, as listed in Table 2-34. One 3-mm long zebra mussel was found attached to the 78-mm long Mapleleaf. There were six dead mussel species, including Butterfly, Giant floater, Pimpleback, Threeridge, Wabash pigtoe, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

35. Quadrat 5-B (UTM: NAD 27 and Zone 15 - 676218E/4589403N)

This site, shown as Q5 in Figure 1-2, was located near Sec 3, where flow depth was 9.5 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 5-B was the second quadrant sample which had eight live mussels, including Hickorynut, Mapleleaf, Pimpleback, Threehorn wartyback, and Washboard, as listed in Table 2-35. Three 3-mm long zebra mussels were found attached to the 50-mm long Threehorn wartyback. There were six dead mussel species, including Fawnsfoot, Fragile papershell, Mapleleaf, Plain pocketbook, Threeridge, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

36. Quadrat 5-C (UTM: NAD 27 and Zone 15 - 676218E/4589403N)

This site, shown as Q5 in Figure 1-2, was located near Sec 3, where flow depth was 9.5 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 5-C was the third quadrant sample which had nine live mussels, including Mapleleaf, Pimpleback, Threehorn wartyback, and Threeridge, as listed in Table 2-36. No live zebra mussels were found attached to these live mussels. There were four dead mussel species, including Hickorynut, Threeridge, Wabash pigtoe, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

37. Quadrat 5-D (UTM: NAD 27 and Zone 15 - 676218E/4589403N)

This site, shown as Q5 in Figure 1-2, was located near Sec 3, where flow depth was 9.5 ft. A Quadrat sample was obtained by a 15-min. dive which was divided into four quadrants. Quadrat 5-D was the forth quadrant sample which had fivee live mussels, including Mapleleaf, Pimpleback, and Washboard, as listed in Table 2-37. Two 3-mm long live zebra mussels were found attached to the 83-mm long Mapleleaf, and two 3-mm long live zebra mussels attached to the 20-mm long Pimpleback. There were six dead mussel species, including Hickorynut, Giant floater, Pimpleback, Threehorn wartyback, Wabash pigtoe, and Washboard, which were all noted as WDs. Neither their lengths nor ages were recorded. The substrate material was not recorded.

64 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 3:05 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Eric Giebelstein, Personnel Steve Johnson, Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.5 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676218E 4589403N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 5-A No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 55 6 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 3 78 10 68 9 40 5 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 27 4 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 2 42 4 46 4 Amblema plicata THREERIDGE 1 36 4 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 8 SPECIES 5 Note: No live Zebs were found

Table 2-34 Size and age distributions of live mussels collected in Quadrat 5-A

65 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 3:05 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.5 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676218E 4589403N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 5-B No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT 1 41 4 Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 37 2 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 37 5 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 4 504404161161 Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 142 9 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 8 SPECIES 5 Note: No live Zebs were found

Table 2-35 Size and age distributions of live mussels collected in Quadrat 5-B

66 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 3:05 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.5 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676218E 4589403N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 5-C No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 30 3 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 1 50 5 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN 4 454474402403 Amblema plicata THREERIDGE 3 70 5 50 4 32 2 Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 9 SPECIES 4 Note: No live Zebs were found

Table 2-36 Size and age distributions of live mussels collected in Quadrat 5-C

67 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006 Date 13-Sep-06 Time 3:05 P.M. (15-min. dive) Bob Schanzle, Rich Lewis, Bernard Schonhoff, Jon Christensen, Steve Johnson, Personnel Nicole McVay, Tatsuaki Nakato Weather/Temp Cloudy/Not recorded Datum Zone Easting Northing Note: d = 9.5 ft -- 0.25 sq m UTM Coordinates NAD 27 15 676218E 4589403N Quadrats Survey Method Brail Pollywog Dive Quadrats Photos Survey ID Quadrat 5-D No Photos Bed Material Not recorded STATUS #, Length (mm) & Age (yrs)

Common Name IL SPECIES IA WI FED Number #1 L (mm) L #1 Yrs #1 (mm) L #2 Yrs #2 (MM) L #3 Yrs #3 (mm) L #4 Yrs #4 (mm) L #5 Yrs #5 Ligumia recta BLACK SANDSHELL T Ellipsaria lineolata BUTTERFLY ETT Truncilla truncata DEERTOE Lampsilis siliquoidea FAT MUCKET Truncilla donaciformis FAWNFOOT Anodonta suborbiculata FLAT FLOATER Leptodea fragilis FRAGILE PAPERSHELL Pyganodon grandis GIANT FLOATER Obovaria olivaria HICKORYNUT Lampsilis higginsi HIGGINS' EYE EEEE Toxolasma parvus LILLIPUT Quadrula quadrula MAPLELEAF 1 83 6 Quadrula metanevra MONKEYFACE T Actinonaias ligamentina MUCKET Utterbackia imbecillis PAPER PONDSHELL Fusconaia flava PIGTOE, WABASH Quadrula pustulosa PIMPLEBACK 3 30 5 30 3 20 2 Potamilus alatus PINK HEELSPLITTER Potamilus ohiensis PINK PAPERSHELL Tritogonia verrucosa PISTOLGRIP (BUCKHORN) TE Lampsilis cardium POCKETBOOK Arcidens confragosus ROCKSHELL T Pleurobema coccineum ROUND PIGTOE E Elliptio dilatata SPIKE T Strophitus undulatus STRANGE FLOATER T Obliquaria reflexa THREEHORN Amblema plicata THREERIDGE Quadrula nodulata WARTYBACK T Megalonaias nervosa WASHBOARD 1 68 4 Lasmigona complanata WHITE HEELSPLITTER Lampsilis teres YELLOW SANDSHELL EE Fusconaia ebena EBONYSHELL ET Strophitus undulatus SQUAWFOOT T TOTAL (Live Mussels) 5 SPECIES 3 Note: No live Zebs were found

Table 2-37 Size and age distributions of live mussels collected in Quadrat 5-D

68 38. Muskrat Midden Site (UTM: NAD 27 and Zone 15 - 676418E/4589598N)

During the mussel surveys on 12 and 13 September 2006, a muskrat midden was discovered on the Iowa shore between Sec 5 and Sec 6 (see Figure 1-3). The authors of this report visited the midden site on 14 September 2006 to find out the number of mussel species in the midden. We found seventeen species of freshwater mussels that are listed in Table 2-38. The shells collected in the midden are shown in Photo 2-21. Only three species in this list, Round pigtoe (Pleurobema coccineum), Slough sandshell (Lampsilis teres teres) and Yellow sandshell (Lampsilis teres anodontoides) were not found in the river mussel surveys which are described in Section 1 through Section 37. Although five Muckets (Actinonaias ligamentina) were found in the river surveys, no Mucket was found in the midden.

Date 14-Sep-06 Location Mississippi River: Pool 16 Right Bank UTM (NAD 27) 15676418E/4589598N NO. Species Common Name 1 Lampsilis teres teres Slough sandshell 2 Lampsilis teres anodontoides Yellow sandshell 3 Lampsilis cardium Plain pocketbook 4 Pyganodon grandis Giant floater 5 Amblema plicata Threeridge 6 Ellipsaria lineolata Butterfly 7 Fusconaia flava Wabash pigtoe 8 Leptodea fragilis Fragile papershell 9 Megalonaias nervosa Washboard 10 Obliquaria reflexa Threehorn wartyback 11 Obovaria olivaria Hickorynut 12 Pleurobema coccineum Round pigtoe 13 Quadrula nodulata Wartyback 14 Quadrula pustulosa Pimpleback 15 Quadrula quadrula Mapleleaf 16 Quadrula metanevra Monkeyface 17 Truncilla truncata Deertoe

Table 2-38 List of mussel species found on 14 September 2006 at a muskrat midden located just upstream from Quadrat 3 site on the right bank of the Mississippi River

Photo 2-21 Seventeen different dead mussel shells found in a muskrat midden in Fairport (UTM Datum, NAD 27, Zone 15: 676418E/4589598N)

70 III. SUMMARY OF OVERALL SURVEY RESULTS

The overall results of the present mussel surveys can be summarized as follows:

1. Sixteen different live freshwater mussel species were found in the mussel survey area along the right bank of the Mississippi River near Fairport, extending riverward approximately 160 m from the Iowa shoreline and encompassing streamwise approximately 800 m (see Figure 1-2). 2. Seventeen freshwater mussel species were identified in a muskrat midden located on the Iowa shoreline, approximately 150 m downstream from the upstream-most survey boundary (see Figure 1-2 and Photo 2-21). Only three species, Round pigtoe (Pleurobema coccineum), Slough sandshell (Lampsilis teres teres) and Yellow sandshell (Lampsilis teres anodontoides) were not found live in the river mussel surveys. Mucket (Actinonaias ligamentina), five of which were found in the river surveys, was not found in the midden. It is believed that those adult Mucket mussels are large and difficult to be harvested by muskrats. 3. The river mussel surveys, including two pollywogs, fifteen dives, and five quadrats, yielded a total of 253 live mussels. The number of live mussels collected at each survey site is shown in Table 3-1. 4. The most common species found was Threeridge (Amblema plicata: 17.8%) followed by Pimpleback (Quadrula pustulosa: 17.4%), Threehorn wartyback (Obliquaria reflexa: 15.8%), Washboard (Megalonaias nervosa: 12.3%), and Mapleleaf (Quadrula quadrula: 12.3%). Frequencies of the other eleven species were less than 6.3%, as can be seen in Table 3-1. The frequency and percentage distributions of the live mussel species identified in the surveys are shown in Figures 3-1 and 3-2, respectively. 5. Age counts of the 253 live mussels sampled were obtained, as shown in Table 3-2, and their distribution is shown in Figure 3-3, in which individual percentage occurrences are also included. The highest age count occurred at age four (55 counts), followed by age six (43 counts) and age five (40 counts). An age group between 4-yrs old and 6-yrs old occupied 54.5% of the total population; that between 3-yrs old and 7-yrs old 78.2%; that between 2-yrs old and 9-yrs old 97.5%; and that between 1-yr old and 10-yrs old 99.6%. 6. The youngest, 1-yr old, live mussels were found at Q3 (one Threehorn wartyback (Obliquaria reflexa)) and at Q5 (two Threehorn wartybacks (Obliquaria reflexa)). The oldest live mussel was found in Dive-1, which was a 14-yr old Plain pocketbook (Lampsilis cardium). The peak age of 4-yrs old indicates that river hydraulic conditions and host-fish populations for the sixteen mussel species found were very favorable to the mussel reproduction in 2002. A lack of older mussels in the samples makes us speculate that zebra mussels could have had something to do with it. River stage records near Fairport, Iowa at River Mile 463.5 that were available from the U.S. Army Corps of Engineers, Rock Island District web site were downloaded and plotted in Figure 3-4. Extended spring floods occurred in

71 Pool 16 in 2002. However, it is extremely difficult to relate simply these flood events to the high mussel production because spring floods occur on an annual basis. 7. Rather high live-mussel population densities were observed at three Quadrats, Q2, Q3, and Q5, which were 26, 31, and 30 in 1 m2, respectively, as shown in Table 3-3. Flow depths at these three Quadrat sites were very similar, and were 9.4 ft, 9.7 ft, and 9.5 ft, respectively. The average flow depth at these three sites was 9.53 ft. This flow depth corresponded to a river-bed elevation of 535.32 ft above mean sea level (MSL 1912) or 163.17 m above MSL. Population densities at Q1 and Q4 were only 8 mussels/m2 and 7 mussels/m2, respectively, and the flow depths were 8.3 ft and 11.8 ft, respectively. The river stage was very stable during the surveys and the water-surface elevation was 544.85 ft above MSL or 166.07 m above MSL. 8. Either of Quadrat sites, Q2, Q3, and Q5, would be an ideal site for relocating Higgins eye (Lampsilis higginsii) mussels because of the high population density. Their UTM locations are

Q2: UTM: NAD 27 and Zone 15 - 676324E/4589471N; Q3: UTM: NAD 27 and Zone 15 - 676400E/4589532N; and, Q5: UTM: NAD 27 and Zone 15 - 676218E/4589403N

9. Furthermore, two additional sites, D7 and D8, would be the candidates for relocating Higgins eye (Lampsilis higginsii) mussels because of the high population density. Their UTM locations are

D7: UTM: NAD 27 and Zone 15 - 676191E/4589397N; and, D8: UTM: NAD 27 and Zone 15 - 676209E/4589372N

10. It is advised that substrate samples be collected at these sites, Q2, Q3, Q5, D7, and D8, prior to the relocation to ensure that the river bottom is suitable for young Higgins eye (Lampsilis higginsii) mussels. Fine sediment substrate sites should be avoided.

72 LACMRERS Mussel Survey Data Sheet Location Mississippi River Fairport Site (Approximately RM 463.5~464.1) -- 9/12-9/13 2006

SPECEIS/Common Name IL IA

WI % FED Total Dive 1 Dive 2 Dive 3 Dive 4 Dive 5 Dive 6 Dive 7 Dive 8 Dive 9 Dive 10 Dive 11 Dive 12 Dive 13 Dive 14 Dive 15 Quadrat 1 Quadrat 2 Quadrat 3 Quadrat 4 Quadrat 5 Quadrat Pollywog 1 Pollywog 2 Pollywog

Ligumia recta/ BLACK SANDSHELL T 11 2 0.8 Ellipsaria lineolata/ BUTTERFLY ETT 21 1 4 1.6 Truncilla truncata/ DEERTOE 1 1 0.4 Pyganodon grandis/ GIANT FLOATER 1 1 0.4 Obovaria olivaria/ HICKORYNUT 21 11 211 113 216 6.3 Quadrula quadrula/ MAPLELEAF 11112131257631 12.3 Quadrula metanevra/ MONKEYFACE T 22 1 11 1 8 3.2 Actinonaias ligamentina/ MUCKET 211116 2.4 Fusconaia flava/ PIGTOE, WABASH 2 11133112 4.7 Quadrula pustulosa/ PIMPLEBACK 211 1 1 121252 8101644 17.4 Lampsilis cardium/ POCKETBOOK 2121 1 7 2.8 Strophitus undulatus/ SQUAWFOOT 1 1 0.4 Obliquaria reflexa/ THREEHORN 1 423 2 321 18121040 15.8 Amblema plicata/ THREERIDGE 34 311 362 4 212 45 445 17.8 Quadrula nodulata/ WARTYBACK T 11114 1.6 Megalonaias nervosa/ WASHBOARD 1434 13 2 141 14 231 12.3 TOTAL (Live Mussels) 9 5 7 10149 2 1011182 6 8 6 13165 8 26317 30253100.0 MUSSEL SPECIES 525585268714439734776616

Substrate Material Sand Sand Sand Silt/Clay Sand/Silt Sandy Silt Sandy Sand/Gravel Sand/Gravel Sand/Gravel Sand/Gravel Sand/Gravel Not recorded Not recorded Not recorded Not recorded Not recorded Not recorded Sand/Gravel/Cobble Sand/Gravel/Cobble Sand/Gravel/Cobble Sand/Gravel/Cobble

Sand/Gravel with Silt on Top on Silt with Sand/Gravel

Table 3-1 Summary of live mussels and substrate materials collected

Age (yrs) Site I.D. 1234567891011121314 P1 00031230000000 P2 00022010000000 D1 00010031010001 D2 00011431000000 D3 00024620000000 D4 01012212000000 D5 00100001000000 D6 00322102000000 D7 00114111200000 D8 00143313300000 D9 00010000100000 D10 00030010200000 D11 03221000000000 D12 01310100000000 D13 00142330000000 D14 01221136000000 D15 00100220000000 Q1 00103120100000 Q2 02335831100000 Q3 11584640200000 Q4 03130000000000 Q5 242115200310000 Total3162755404333181520001

Table 3-2 Summary of age counts of live mussels at individual survey sites

73 Quadrat 1 Summary (13 September 2006) ID Species Common Name Number in square meter 1 Obovaria olivaria HICKORYNUT 1 2 Quadrula quadrula MAPLELEAF 5 3 Quadrula metanevra MONKEYFACE 1 4 Obliquaria reflexa THREEHORN 1 Total 8

Quadrat 2 Summary (13 September 2006) ID Species Common Name Number in square meter 1 Obovaria olivaria HICKORYNUT 1 2 Actinonaias ligamentina MUCKET 1 3 Fusconaia flava PIGTOE, WABASH 3 4 Quadrula pustulosa PIMPLEBACK 8 5 Obliquaria reflexa THREEHORN 8 6 Amblema plicata THREERIDGE 4 7 Megalonaias nervosa WASHBOARD 1 Total 26

Quadrat 3 Summary (13 September 2006) ID Species Common Name Number in square meter 1 Obovaria olivaria HICKORYNUT 3 2 Quadrula quadrula MAPLELEAF 7 3 Quadrula pustulosa PIMPLEBACK 10 4 Obliquaria reflexa THREEHORN 1 5 Amblema plicata THREERIDGE 5 6 Quadrula nodulata WARTYBACK 1 7 Megalonaias nervosa WASHBOARD 4 Total 31

Quadrat 4 Summary (13 September 2006) ID Species Common Name Number in square meter 1 Truncilla truncata DEERTOE 1 2 Quadrula metanevra MONKEYFACE 1 3 Fusconaia flava PIGTOE, WABASH 1 4 Quadrula pustulosa PIMPLEBACK 1 5 Lampsilis cardium POCKETBOOK 1 6 Obliquaria reflexa THREEHORN 2 Total 7

Quadrat 5 Summary (13 September 2006) ID Species Common Name Number in square meter 1 Obovaria olivaria HICKORYNUT 2 2 Quadrula quadrula MAPLELEAF 6 3 Quadrula pustulosa PIMPLEBACK 6 4 Obliquaria reflexa THREEHORN 10 5 Amblema plicata THREERIDGE 4 6 Megalonaias nervosa WASHBOARD 2 Total 30

Table 3-3 Summary of live mussels collected by five Quadrat surveys

74 Total Number of Live Mussels = 253 50

TOTAL NUMBER OF LIVE SPECIES NUMBER OF LIVE TOTAL 0 L Y E R F T H K T E K L L E A CE E O E O S CK O RN C RD T NUT E A A A O O DG A A H RF RT A Y L F B B F B S E E B HO RI O E O R Y UCK A E T E Y D T L L E L W E T O P /M W E HB N DE F P K R A UT / A a , UA S a T CK NK n E IM C HRE A A S /B t I M ti Q HRE a N / O O P O T W K a A /H a n / /S / /T / /W t c I l /M e T a /P a a C a n ia u s x ta t a l G r r a m IG s u a s A o ru / d r o m t le a l L e t s a v a P l u a f c u o i iv a e g / u i l li v B in a d l u li a t d u re d r / l ll n o q n v s r d p o e a i a s u a a n n t ia c a a a t a la c n i c r n r i l e i f p u r s e g r u a s a ma la r a u a r m a la i s u e ia s r n v n i il l ru a p T d a o a u s tu iq b a li o o a l r i l d n l d b u in n d p h b m a mi o ru t o lo E O c a m p O A u u n Q d c u a o a ig a a A s r Q g u u Q L t e L g S y Q F M P

Figure 3-1 Frequency distribution of sixteen live mussel species

Total Number of Live Mussels = 253 20

PERCENTAGE OF TOTAL LIVE MUSSELS LIVE TOTAL OF PERCENTAGE 0 L Y E R F T H K T E K L L O E A CE E S CK O O RN C RD T NUT E A A A A A HE L O O IDG RF RT A F B B B F HO B O E E O RY E Y UCK A E T R Y T L W E E L O E M L E HB NDS F P K / W P RT DE A a , S A UT / N M UA A B a CK in E I CK HRE A S / t NT /M O t Q HRE a HI n O P O S /T T /W W ta A / la M T / P / / / c I / e a s a a a a CK la n ia u G s x t t u G r r ra m I m/ tu e a s A o r / a d a lo l a l o L e t is a v P iu la f ic u v iv e ig / u e l d r B in a d l u l a t d u r p / l ll n o q n v s r d o e a i a s u a a n n t ia c a a a t a la c n ri c r n r i l i f p u a s e g r u a s a ma l r a u a r me a la li s u e u ia s r n v d n i i u l r a a p T o a o a u s t iq li o a l r i l d n l d b u u in n d h b mb a o mi o r t o a p u l u E O Q d c c mp O A a n s u a o Q ig a a A L tr g g u u Q e L F S y Q M P

Figure 3-2 Percentage distribution of sixteen live mussel species

60 21.7%

17.0% 40 15.8% 13.0% 30 10.7%

20 7.1% 6.3% 5.9% Number of Live Mussels Number of 10

1.2% 0.8% 0% 0% 0% 0.4% 0 02468101214 Age (yrs)

Figure 3-3 Age distribution of 253 live mussels

25 Record Flood Stage @ 24.74 ft on 9 July 1993

20 Flood Stage @ 14.0 ft

10 Gage Zero: 535.16 ft MSL 1942 RIVER STAGE IN FAIRPORT IN FT Flat Pool Stage: 9.80 ft Gage Located @ RM 463.5 5 1990 1992 1994 1996 1998 2000 2002 2004 2006 YEAR

Figure 3-4 Daily Mississippi River stage records near Fairport, Iowa from 1 January 1990 to 30 August 2007 – data source: http://www2.mvr.usace.army.mil/WaterControl/new/layout.cfm

76 IV. REFERENCES

1. Cummings, K.S., and Mayer, C.A., 1992. “Field guide to freshwater mussels of the Midwest,” Illinois Natural History Survey, Manual 5, 194 pp. 2. Iowa State University, Iowa Geographic Map Server site: http://komodo.gis.iastate.edu/ 3. National Geographic, 2002. “IOWA - Seamless U.S.G.S. topographic maps on CD-ROM powered by TOPO!” Version 2.7.5. 4. U.S. Army Corps of Engineers, Rock Island District web site: http://www2.mvr.usace.army.mil/WaterControl/new/layout.cfm