SNAPC4 (NM 003086) Human Tagged ORF Clone – RC219682

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC219682

SNAPC4 (NM_003086) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: SNAPC4 (NM_003086) Human Tagged ORF Clone Tag: Myc-DDK Symbol: SNAPC4 Synonyms: PTFalpha; SNAP190 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC219682 representing NM_003086 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC

ATGGATGTAGATGCTGAAAGAGAGAAGATAACACAGGAGATCAAGGAGCTGGAAAGGATTTTGGATCCCG GCTCCTCGGGCTCCCACGTGGAGATCTCAGAATCAAGTCTCGAGTCAGATTCTGAAGCAGATTCACTGCC TTCTGAGGACTTGGATCCTGCCGATCCCCCGATCTCGGAAGAAGAAAGGTGGGGCGAAGCCAGCAATGAC GAGGACGATCCCAAGGATAAAACCCTCCCTGAAGACCCAGAAACCTGCCTGCAGCTGAACATGGTCTACC AGGAGGTCATCCAGGAGAAGCTGGCTGAGGCCAACCTGCTGCTGGCCCAGAACCGGGAGCAGCAGGAGGA ACTCATGAGGGATCTGGCTGGGTCCAAAGGCACCAAGGTGAAAGACGGCAAAAGCCTGCCCCCAAGCACA TACATGGGGCACTTCATGAAGCCGTATTTCAAGGACAAGGTCACGGGCGTGGGGCCACCTGCCAACGAGG ACACACGAGAGAAGGCTGCCCAGGGGATCAAGGCTTTCGAGGAGCTCCTTGTGACCAAATGGAAAAACTG GGAAAAGGCCTTGCTCCGAAAGTCAGTGGTGAGTGACCGCCTGCAGCGATTGCTTCAGCCCAAGTTACTG AAGCTCGAGTACTTGCACCAGAAGCAGAGCAAAGTCTCCAGTGAGCTGGAGAGGCAAGCCCTGGAGAAGC AGGGCAGGGAAGCCGAGAAGGAGATCCAGGACATCAACCAGCTTCCAGAAGAGGCCTTGCTGGGAAACAG GCTGGACAGCCACGACTGGGAGAAGATTTCCAATATTAACTTTGAAGGCAGCCGCAGTGCAGAGGAGATC CGGAAGTTCTGGCAGAACTCGGAGCACCCCAGCATCAACAAGCAGGAGTGGAGCAGGGAGGAGGAGGAGC GGCTGCAGGCGATCGCGGCTGCACACGGCCACCTGGAGTGGCAGAAGATTGCAGAGGAGCTGGGGACCAG CCGCAGCGCCTTCCAGTGCCTGCAGAAATTCCAGCAGCACAACAAAGCTCTGAAACGCAAGGAGTGGACA GAGGAGGAGGACCGCATGCTCACGCAGCTGGTGCAGGAGATGCGCGTCGGCAGCCACATCCCCTACCGCA GAATTGTCTACTATATGGAAGGGAGAGACTCCATGCAGCTGATCTACCGATGGACCAAGAGCTTGGATCC TGGTCTGAAGAAGGGTTACTGGGCCCCGGAGGAAGATGCTAAGTTGCTTCAAGCTGTTGCCAAATACGGG GAGCAGGATTGGTTTAAAATCCGGGAAGAGGTGCCAGGTAGGAGCGATGCCCAGTGCCGAGATCGGTATC TCAGGAGATTACATTTCAGCTTGAAAAAGGGTCGGTGGAATTTAAAAGAAGAGGAACAGTTAATTGAATT AATAGAAAAATATGGTGTCGGTCACTGGGCAAAAATAGCTTCTGAGCTGCCCCATCGGTCTGGCTCCCAG TGTCTGAGCAAGTGGAAGATCATGATGGGGAAGAAGCAGGGTCTCCGGAGGCGGCGGCGGAGGGCCCGTC

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 SNAPC4 (NM_003086) Human Tagged ORF Clone – RC219682

ACAGCGTCCGGTGGAGCTCTACCAGCAGCAGCGGCAGCAGCAGTGGCAGCAGTGGAGGGAGCAGCAGCAG CAGCAGCAGCAGCAGCGAGGAGGACGAGCCAGAGCAGGCGCAGGCCGGGGAGGGTGACAGAGCGCTGCTG TCCCCACAGTACATGGTCCCGGACATGGACCTGTGGGTTCCTGCCAGGCAGAGCACCAGCCAGCCATGGA GAGGAGGGGCAGGGGCCTGGCTGGGAGGCCCCGCTGCCTCCCTCAGCCCTCCCAAGGGGTCCAGTGCCAG CCAGGGCGGCAGCAAGGAAGCTTCCACCACAGCCGCGGCTCCTGGAGAGGAGACGAGTCCGGTGCAGGTC CCTGCCAGGGCCCACGGCCCTGTCCCGAGGTCTGCCCAGGCCTCCCACTCAGCAGACACTCGCCCGGCGG GCGCAGAGAAGCAGGCCCTGGAGGGTGGGAGGCGTCTGCTGACAGTGCCTGTGGAGACCGTGCTGAGGGT GCTCAGGGCCAACACGGCTGCTCGGAGCTGCACACAGAAAGAGCAGCTGAGGCAGCCACCCCTGCCCACC TCATCCCCAGGGGTCAGCTCTGGTGACAGCGTGGCCCGATCCCATGTGCAGTGGCTACGGCACAGAGCCA CCCAGAGTGGGCAGCGGCGCTGGAGACACGCTCTGCACCGGAGGCTCCTGAACCGCAGGCTGCTGCTGGC TGTGACCCCTTGGGTAGGGGACGTTGTCGTGCCCTGCACACAGGCTTCCCAGAGACCCGCCGTAGTGCAG ACTCAAGCGGATGGCCTCAGGGAGCAGCTGCAGCAGGCCCGCCTGGCCAGCACCCCTGTGTTTACCCTGT TTACCCAGCTGTTCCACATCGATACTGCCGGCTGCTTGGAGGTCGTCCGAGAGAGGAAGGCCCTGCCACC CAGGCTGCCCCAGGCTGGTGCTCGGGACCCACCAGTTCATCTTCTGCAGGCATCCTCAAGTGCCCAGAGC ACCCCAGGCCACCTCTTCCCAAACGTGCCGGCTCAAGAAGCCTCAAAGAGTGCCAGCCACAAAGGGAGCC GAAGACTGGCGTCCAGCCGGGTGGAGCGCACCCTACCCCAGGCGTCCCTGCTGGCTTCAACCGGCCCCCG GCCCAAGCCCAAGACTGTGTCGGAGCTGCTTCAGGAGAAGCGGCTTCAGGAGGCCCGTGCCAGGGAGGCC ACCCGGGGCCCGGTGGTGCTCCCGTCCCAGCTGCTGGTCTCCTCGTCTGTGATCCTCCAGCCCCCTCTAC CACACACCCCACACGGCCGCCCAGCCCCGGGTCCCACCGTCTTAAATGTACCGCTCTCTGGGCCTGGGGC CCCCGCAGCAGCCAAACCTGGCACTTCTGGCTCCTGGCAGGAGGCTGGGACTTCAGCCAAGGACAAGAGA CTCTCCACCATGCAAGCCCTGCCCCTCGCTCCTGTCTTCTCAGAGGCCGAAGGCACAGCCCCTGCTGCTT CCCAAGCCCCTGCCCTGGGCCCCGGCCAGATCTCTGTGAGCTGCCCCGAGAGTGGTCTCGGACAGTCTCA GGCCCCCGCTGCATCCCGGAAGCAGGGCCTGCCTGAGGCGCCACCCTTTCTCCCCGGAGCCCCCAGCCCC ACCCCACTGCCCGTCCAGCCCCTCAGCCTGACGCACATAGGAGGGCCACATGTGGCGACCAGTGTCCCCC TGCCTGTCACCTGGGTGCTCACAGCCCAGGGGCTTCTCCCTGTTCCTGTACCAGCTGTGGTGAGCCTTCC CAGGCCAGCAGGGACCCCTGGCCCCGCAGGGCTGCTGGCCACTCTGCTGCCTCCCCTGACTGAGACTCGG GCGGCCCAGGGCCCCAGGGCCCCAGCGTTGAGCAGCTCTTGGCAGCCCCCAGCCAATATGAACAGGGAAC CGGAGCCTTCCTGCAGGACAGACACCCCAGCTCCTCCCACACACGCCCTCTCCCAAAGTCCTGCAGAAGC GGATGGCAGTGTGGCCTTTGTCCCTGGAGAGGCCCAGGTGGCCAGGGAGATACCTGAGCCCAGGACGTCC TCCCACGCTGACCCTCCTGAAGCAGAACCCCCTTGGTCCGGGAGGCTGCCAGCCTTCGGTGGTGTCATCC CAGCAACTGAGCCAAGGGGGACGCCGGGGTCCCCCTCAGGGACACAGGAGCCCAGGGGGCCTCTGGGCCT GGAGAAGCTGCCCCTGCGCCAGCCTGGGCCTGAGAAGGGGGCCCTGGACCTGGAGAAGCCGCCCCTACCC CAGCCTGGGCCTGAGAAGGGGGCCCTGGACCTGGGCCTGCTGTCCCAGGAGGGCGAGGCAGCCACACAGC AGTGGCTGGGGGGCCAGCGGGGGGTGCGTGTGCCTCTTCTGGGCAGCAGACTGCCCTATCAGCCCCCAGC CCTGTGCAGCCTGCGAGCTCTGTCCGGTCTCCTACTCCACAAGAAGGCCCTGGAGCACAAGGCCACCTCC CTGGTGGTGGGGGGCGAGGCTGAGCGGCCGGCCGGAGCACTGCAAGCCTCACTGGGGCTGGTGCGGGGGC AGCTCCAGGACAACCCGGCCTACCTCCTGTTGCGGGCGCGGTTCCTGGCAGCCTTCACCCTCCCTGCGCT CCTGGCCACCCTGGCCCCCCAAGGCGTCCGCACCACCCTCTCAGTACCTTCGAGGGTGGGCTCTGAGAGT GAGGATGAAGACCTCCTGAGTGAGCTGGAACTTGCAGACAGGGACGGGCAGCCGGGCTGCACGACAGCCA CATGCCCCATTCAGGGAGCCCCAGACTCTGGTAAATGCTCTGCTTCCTCCTGCCTGGATACTTCTAATGA CCCTGACGACCTGGACGTGCTCAGAACCCGGCATGCCAGGCACACCCGGAAGCGGAGGCGGCTGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 SNAPC4 (NM_003086) Human Tagged ORF Clone – RC219682

Protein Sequence: >RC219682 representing NM_003086 Red=Cloning site Green=Tags(s)

MDVDAEREKITQEIKELERILDPGSSGSHVEISESSLESDSEADSLPSEDLDPADPPISEEERWGEASND EDDPKDKTLPEDPETCLQLNMVYQEVIQEKLAEANLLLAQNREQQEELMRDLAGSKGTKVKDGKSLPPST YMGHFMKPYFKDKVTGVGPPANEDTREKAAQGIKAFEELLVTKWKNWEKALLRKSVVSDRLQRLLQPKLL KLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEI RKFWQNSEHPSINKQEWSREEEERLQAIAAAHGHLEWQKIAEELGTSRSAFQCLQKFQQHNKALKRKEWT EEEDRMLTQLVQEMRVGSHIPYRRIVYYMEGRDSMQLIYRWTKSLDPGLKKGYWAPEEDAKLLQAVAKYG EQDWFKIREEVPGRSDAQCRDRYLRRLHFSLKKGRWNLKEEEQLIELIEKYGVGHWAKIASELPHRSGSQ CLSKWKIMMGKKQGLRRRRRRARHSVRWSSTSSSGSSSGSSGGSSSSSSSSSEEDEPEQAQAGEGDRALL SPQYMVPDMDLWVPARQSTSQPWRGGAGAWLGGPAASLSPPKGSSASQGGSKEASTTAAAPGEETSPVQV PARAHGPVPRSAQASHSADTRPAGAEKQALEGGRRLLTVPVETVLRVLRANTAARSCTQKEQLRQPPLPT SSPGVSSGDSVARSHVQWLRHRATQSGQRRWRHALHRRLLNRRLLLAVTPWVGDVVVPCTQASQRPAVVQ TQADGLREQLQQARLASTPVFTLFTQLFHIDTAGCLEVVRERKALPPRLPQAGARDPPVHLLQASSSAQS TPGHLFPNVPAQEASKSASHKGSRRLASSRVERTLPQASLLASTGPRPKPKTVSELLQEKRLQEARAREA TRGPVVLPSQLLVSSSVILQPPLPHTPHGRPAPGPTVLNVPLSGPGAPAAAKPGTSGSWQEAGTSAKDKR LSTMQALPLAPVFSEAEGTAPAASQAPALGPGQISVSCPESGLGQSQAPAASRKQGLPEAPPFLPGAPSP TPLPVQPLSLTHIGGPHVATSVPLPVTWVLTAQGLLPVPVPAVVSLPRPAGTPGPAGLLATLLPPLTETR AAQGPRAPALSSSWQPPANMNREPEPSCRTDTPAPPTHALSQSPAEADGSVAFVPGEAQVAREIPEPRTS SHADPPEAEPPWSGRLPAFGGVIPATEPRGTPGSPSGTQEPRGPLGLEKLPLRQPGPEKGALDLEKPPLP QPGPEKGALDLGLLSQEGEAATQQWLGGQRGVRVPLLGSRLPYQPPALCSLRALSGLLLHKKALEHKATS LVVGGEAERPAGALQASLGLVRGQLQDNPAYLLLRARFLAAFTLPALLATLAPQGVRTTLSVPSRVGSES EDEDLLSELELADRDGQPGCTTATCPIQGAPDSGKCSASSCLDTSNDPDDLDVLRTRHARHTRKRRRLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Restriction Sites: AscI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 SNAPC4 (NM_003086) Human Tagged ORF Clone – RC219682

Plasmid Map:

ACCN: NM_003086 ORF Size: 4407 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_003086.1, NP_003077.1 RefSeq Size: 5027 bp RefSeq ORF: 4410 bp Locus ID: 6621

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 SNAPC4 (NM_003086) Human Tagged ORF Clone – RC219682

UniProt ID: Q5SXM2, A0A024R8F4 MW: 159.3 kDa Gene Summary: This gene encodes the largest subunit of the small nuclear RNA-activating protein (SNAP) complex. The encoded protein contains a Myb DNA-binding domain, and is essential for RNA polymerase II and III polymerase transcription from small nuclear RNA promoters. A mutation in this gene is associated with ankylosing spondylitis. [provided by RefSeq, Jul 2016]

Product images:

Western blot validation of overexpression lysate (Cat# [LY418907]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219682 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).