Catalogue-2020-V3.Pdf

PORTABLE DEVICES 6 DEVICES 7 STRIPS 9

STRIP ACCESSORIES 9

EBQC ACCESSORIES 10

STANDARDS 10

BUFFERS 12 ASSAY KITS 14 ICON KEY 15

COLORIMETRIC/FLUORIMETRIC ASSAY-KITS 16

OXIDATIVE STRESS LINE 16

CELLULAR LINE 29

APOPTOSIS LINE 31 PROTEIN ASSAY KITS 32

PROTEIN QUANTIFICATION 32

PROTEIN PRECIPITATION REAGENTS 34 STAIN LINE 37

PRODUCT LIST 45

Bioquochem is a biotech company that designs, develops and manufactures kits and devices for rapid and easy quantification of biological parameters or biomarkers. The company is specialized in the study of oxidative stress.

BQCkit brand includes a wide range of laboratory kits, that is continuously growing due to the active work of our R&D department. Something remarkable is that our products are reasonably priced, but always maintaining the highest quality standards. The main lines are: Colorimetric/fluorimetric assay kits o Oxidative Stress and Antioxidant Capacity o Cellular Line: proliferation, apoptosis, Histology Stain Kits Protein Precipitation Reagents bqckit.com Protein Quantification

e-BQC brand includes electrochemical portable devices for the quantification of oxidative stress parameters in different matrixes such as blood, for the evaluation of oxidative stress, or drinks for the analysis of the antioxidant capacity in wine, milk....

e-bqc.com

Bioquochem is also aware of the needs of the industry and research laboratories and focuses its efforts on the constant development of new products. The company is specially committed to offer the best service to our clients, having particular regard to technical assistance before and after acquisition of our products.

Check Bioquochem website for getting to know the lattest Scientici publications using BQC procuts.

Bioquochem is present in more than 20 countries, and is always growing. To check distributors in your country, please refer to bioquochem.com for updated information.

PORTABLE DEVICES

DEVICES eBQC lab eBQC-lab

e-BQC is a portable device, designed to measure, in a direct way, the antioxidant capacity of liquid samples. The result is obtained in less than a minute and with only one drop of sample.

For aqueous based samples or organic- aqueous mix 50:50 The results are given as two charge values: Freedom to use with any aqueous based

solutions or 50:50 aqueous: lipid solutions Q1 (fast antioxidants) : Refers to the antioxidant Distinguish between fast and slow antioxidants. capacity of the compounds with the highest Transform the results to any classical antioxidant rate of free radical scavenging. standard: CEAC, TEAC or GAE in your specific Q2 (slow antioxidant): Refers to the antioxidant experimental conditions. capacity of the compounds with a lower rate of Lower detection limits than traditional methods free radical scavenging. Fast and easy procedure Portable

TECHNICAL SPECIFICATIONS

PARAMETER Q1 (FAST) Q2 (SLOW) QT (TOTAL)

Sensitivity* 0.07 μC/ μM 0.04 μC/ μM 0.10 μC/ μM

Limit of detection* 2 μM 10 μM 12 μM

Limit of quantification* 3 μM 16 μM 19 μM

Limit of blank 1.7 μM 9.2 μM 10.9 μM

Relative Standard 8 % 5 % 5 % Deviation

Linear range* 100-1000 μM 100-1000 μM 100-1000 μM

* Those values are referred to ascorbic acid antioxidant capacity equivalents (CEAC)

SAMPLES eBQC Device has been tested on the following samples:

FOOD: Milk, Honey, Juice, Wine, Beer, Champagne, Coffee, Blueberries BIOLOGIC: Blood, Plasma, Saliva, Urine, Cell Cultures, Cell Extracts, Tissue Homogenates

Please ask for the Validation Report, Mistake user guide for more info regarding the eBQC technical data.

Skin Device and Conductive Hydrogels

New device, eBQC Skin, for direct quantification of Antioxidant Capacity on the skin, directly with the conductive Hydrogels. The conductive hydrogels are designed to measure skin in vivo. Stick them to the skin and let them sit for a short period of time (between 30 sec – 2 minutes) depending on the treatment, then put them in the electrode and measure.

STRIPS eBQC strips ebqc-strips

e-BQC works with disposable strips to dispense the drop on. Available in 4 sizes according to your necessities: 50 units 250 units 500 units 1000 units

STRIP Accessories Adhesive mesh

The adhesive mesh is designed to measure blood directly without any pretreatment. Stick to the electrode and apply the blood on top of it. The mesh will remove the

interferentes without further complications.

EBQC ACCESSORIES Standards Ascorbic acid ST08001

Ascorbic acid or Vitamin C is the strongest natural water- soluble antioxidant, commonly used to measure antioxidant capacity. It is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables or as an additive. Bioquochem offers Ascorbic Acid Standard for its use with the e-BQC device. Recommended uses: To compare the e-BQC results (µC) to classical antioxidant capacity units of Vitamin C Antioxidant Capacity Equivalents (CEAC). It could be used for both biological and food samples. Size: 5x vials of powder

Gallic Acid ST08002

Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples. Recommended uses: To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples. Size: 5x vials of powder

TROLOX ST08003

Trolox is a water soluble analog of tocopherol (Vitamin E) which is a very strong antioxidant, commonly used to measure antioxidant capacity. Vitamin E is present in biological samples, like plasma, and food samples as a natural component of fruit and vegetables Recommended uses: To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples. Size: 5x vials of powder

Antioxidant mix ST08003

One vial of each antioxidants: ascorbic acid, Trolox and gallic acid. Recommended uses: To compare the e-BQC results (µC) to classical antioxidant capacity units of TROLOX Antioxidant Capacity Equivalents (TEAC). It could be used for biological samples. Size: 3 vials, 1 for each antioxidant

Buffers Nutrition buffer ST08005

Buffer solution pH 5.8 ready-to-use. This solution does not contain calcium or magnesium. Maintains the stability of the ascorbic acid in its natural conditions. Recommended uses: To dilute samples of food and beverages, or antioxidant standards. Compatible with e- BQC lab device. Size: 125 ml / 500 ml

Bio-Buffer ST08006

1X filtered PBS solution pH 7.4 ready-to-use. This solution does not contain calcium or magnesium. It has similar physicochemical conditions as the human body, such as pH, osmolarity and ion concentrations. Recommended uses: cell cultures and tissue extracts. Size: 125 ml / 500 ml

General Buffer ST08007

Buffer solution pH 7 ready-to-use. This solution does not contain calcium or magnesium. Recommended uses: To dilute samples with low requirements, or antioxidant standards. Compatible with e-BQC lab device. Size: 125 ml / 500 ml

COMPATIBILITIES BETWEEN BUFFERS AND STANDARDS

Nutrition Buffer BIO-Buffer General Buffer

Antioxidant Ascorbic Acid and Galic Ascorbic Acid, Galic Acid and Ascorbic Acid and Galic Compatibility Acid Trolox Acid

pH 5.8 7.4 7

Sterile No Yes No

To dilute, wash or resuspend To dilute samples of food biological samples of cell and and beverages, or tissue cultures, or antioxidant To dilute samples with low antioxidant standards. standards. It has similar Intended use requirements, or antioxidant Maintains the stability of the physicochemical conditions as standards. ascorbic acid in its natural the human body, such as pH, conditions. osmolarity and ion concentrations.

ASSAY KITS

ICON KEY

Available sizes

Volume of sample required

Types of sample

Linear range of the assay

Detection

Time required for the assay

Limit of detection

Sensitivity

Unique feature

BQC assay kits typically include*:

Standard 96 well plate Instruction manual MSDS

*Except for the kits that are not for 96 well plate detection and/or do not require standard.

COLORIMETRIC/FLUORIMETRIC ASSAY-KITS

Oxidative Stress Line ANTIOXIDANT CAPACITY

DMPD ANTIOXIDANT CAPACITY ASSAY KIT KF01001

This kit is recommended for antioxidant capacity measurements in foods, beverages and biological samples. It measures the antioxidant activity of compounds that are able to transfer hydrogen atoms. In our assay a solution of DMPD in the presence of a suitable oxidant solution, can form a stable and colored radical cation which shows an absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce a discolorations of the solution which is proportional to their amount.

200 tests 10-150 µg/ml 9.5 µM

20 µL Colorimetric (553 nm) 0.25 % Inhibition/ TEAC (µg/ml)

Foods and beverages 15-20 min Very stable radical (12 h). High sensitivity

towards ascorbic acid.

ABTS ANTIOXIDANT CAPACITY ASSAY KIT KF01002

BQCkit ABTS assay kit is recommended for total antioxidant activity of solutions of pure substances, aqueous mixtures and beverages. The assay described here involves the direct production of the blue/ green ABTS•+ chromophore. This has absorption maxima at 734 nm. The addition of antioxidants to the pre-formed radical cation, reduces it ABTS depending on the antioxidant activity and the concentration of the antioxidant.

250 tests 2.5-130 µM 84.89 µM

5 µL Colorimetric (734 nm) 0.0728 % Inhibition/ CEAC (µM)

Biological fluids, cell 15-20 min Independent of pH effect. Very low

lysates, tissue sample volume. homogenates, foods and beverages. 16

FRAP ANTIOXIDANT CAPACITY ASSAY KIT KF01003 The kit is designed for the antioxidant capacity measurement in biological samples. BQCkit FRAP assay kit is recommended for total antioxidant activity of single antioxidants in aqueous solution and added to plasma. The assay described here measures the ferric reducing ability of plasma (FRAP). At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), an intense blue color with an absorption maximum at 593 nm develops.

500 tests 7.5-250 µM 7.5 µM

10 µL Colorimetric (593 nm) 0.0004 OD/ µM FeSO4

Biological fluids 10-15 min Compatible with multiple antioxidant

standards: ascorbic acid, trolox and gallic acid.

FAST FRAP ANTIOXIDANT CAPACITY ASSAY KIT KF01006 BQC FAST FRAP assay kit is a ready-to-use, easy and highly reproducible assay to test TAC on single antioxidants in aqueous solutions, added to plasma and on beverages with the outstanding feature of being faster than the FRAP classical assay. At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), an intense blue color with an absorption maximum at 593 nm develops.

100/200/500 tests 7.5-250 µM 7.5 µM

10 µL Colorimetric (593 nm) 0.0004 OD/ µM FeSO4

Biological fluids 5 min Equal performance than FRAP but one

step procedure with ready-to-use reagents.

ORAC ANTIOXIDANT CAPACITY ASSAY KIT KF01004

The kit is designed for the antioxidant capacity measurement in biological samples. The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein, to result in a change of fluorescent intensity and the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.

96/192/960 tests 1-80 µM 10 µM

20 µL Fluorimetric (485/530 nm) 0.13 OD/ µM

Cell lysates, tissue 2 h Hidrophilic and lipophilic

homogenates, plasma, antioxidants. serum, saliva, foods and beverages

CUPRAC ANTIOXIDANT CAPACITY ASSAY KIT KF01005 CUPRAC (CUPric Reducing Antioxidant Capacity) is a mostly new method which enables the Total Antioxidant Capacity (TAC) measurements hydrophilic as well as hydrophobic samples. The main reagent, the copper (II)- neocuproine (2,9-dimethyl-1,10-phenanthroline), is able to oxidize antioxidants generating a coloured product. The chelation with neocuproine enables a fastened reaction by elevating the redox potential of the reagent. The BQC CUPRAC Antioxidant Capacity Assay Kit is a stable, selective, easy and quick assay that measures all of the significant antioxidants on biological samples at physiological pH.

250 tests 0-2 µM 0.0115 µM

40 µL Colorimetric (450 nm) 1.3 OD/ µM Trolox

Biological fluids 30 min Physiological pH, sensitive towards thiols,

specially GSH

DPPH ANTIOXIDANT CAPACITY ASSAY KIT KF01007 DPPH assay measures the antioxidant activity of compounds that are able to transfer hydrogen atoms. The compound (DPPH•+) a coloured and stable radical cation of purple colour which shows a maximum of absorbance at 517 nm. Antioxidant compounds, which are able to transfer an electron to DMPD•+, cause a discolouration of the solution. This reaction israpid and proportional to the antioxidant capacity of the sample.

100 / 200 tests 100-500 µM 50 µM

20 µL Colorimetric (517 nm) 0.113% Inhibition/ TEAC

Foods, Beverages and 15 min Widely used, easy and fast to perform,

plant extracts high reproducibility.

BQC ANTIOXIDANT CAPACITY KITS COMPARISON

FAST DMPD ABTS FRAP ORAC CUPRAC DPPH FRAP

Fluorimetric Detection Colorimetri Colorimetri Colorimetri Colorimetri (ex= 485 nm; Colorimetri Colorimetri method c (553 nm) c (734 nm) c (593 nm) c (593 nm) em= 528-538 c (450 nm) c (517 nm) nm)

Physiologic Reaction ph Acid Acid Acid Acid Physiological - al

Hydrophilic Hydrophilic Hydrophilic Hydrophilic Hydrophilic or lipophilic Hydrophilic and Hydrophilic Hydrophilic and and and lipophilic substances lipophilic lipophilic lipophilic

Biological samples, cell Biological Biological Biological Type of Food and Food and lysates, tissue samples, Food and samples samples beverages beverages homogenate food and beverages sample (plasma) (plasma) s, food and beverages beverages Type of measureme Endpoint Endpoint Endpoint Endpoint Kinetic Kinetic Endpoint nt

ANTIOXIDANT MOLECULES - ENDOGENOUS

THIOL QUANTIFICATION ASSAY KIT KB03007

The kit is designed to measure free thiols and disulfides in biological samples. BQCkit Thiol Quantification Assay Kit, measures the reduced and oxidized thiol groups in biological samples using Ellman´s Reagent. In the assay, Ellman´s Reagent, reacts with GSH (not GSSG), generating a colorimetric signal at 412 nm, so GSH can be specifically quantified. GSSG is calculated by subtraction of GSH to GSHt.

200 tests 0-16 µM 0.09 µM

20-40 µL Colorimetric (580-620 nm) 0.78 OD/µM

Biological samples, including 1-2 h Quantification of native free

urine, plasma and cell thiols and total free thiols, two extracts different protocols

OXIDATION PRODUCTS

LIPID PEROXIDATION ASSAY KIT KB03002

The kit is designed to determine lipid peroxidation in biological fluids. This measures the MDA and HNE concentrations as an index of lipid peroxidation. Reactions between indoles and aldehydes (MDA and HNE) gives a diindolylalkane (chromophore) whose maximal absorbance is in the 580-620 nm region.

200 tests 0.25-5 nmol 0.2 µM

100 µL Colorimetric (580-620 nm) 0.0097 OD/µM

Any samples containing lipid 1 h Measures both MDA and HNE

hydroperoxides

MDA - TBARS ASSAY KIT KB03016

The Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. The samples containing MDA or the MDA standards are reacted with TBA. This method, is convenient to determine the relative lipid peroxide content of samples, including plasma, serum, cell culture supernatants and urine samples. Multi-unsaturated lipids, are most likely to form peroxides, and so they are the most reactive lipids in the TBARs assay. TBARs assay is commonly used to compare one set of samples to another. After the incubation, the samples and standards can be read spectrophotometrically or fluorometrically. The MDA concentration in the samples is then calculated by comparison with the MDA standard curve.

100/200/400 tests Colorimetric: 2.5-50 µM, Colorimetric: 9.72 µM

Fluorimetric: 0.25-5 µM

150 µL Colorimetric (532 nm) Colorimetric: 0.0037 OD/µM

Fluorimetric (Ex= 532 nm; Em= 553 nm) Plasma, serum, cell Fluorimetric and Colorimetric Protocols

culture supernatants and standards. and urine samples PROTEIN CARBONYLATION ASSAY KIT KB03008

The kit is designed for the measurement of oxidized proteins in many kind of samples. Protein carbonylation provides an integrated assessment of oxidative damage. The most employed is based on 2,4-dinitrophenylhydrazine (DNPH). This molecule reacts with carbonyl groups leading to the formation of the stable 2,4-dinitrophenylhydrazone. The dinitrophenyl group (DNP) can be detected and quantified spectrophotometrically at 365–375 nm.

100 tests 1-10 mg/mL of protein Not available

200 µL Colorimetric (365- 375 Not available

nm) Plasma, serum, tissue 1 h Quantification of native free thiols and

homogenates and cell total free thiols lysates.

REACTIVE SPECIES

NITRITE DETERMINATION ASSAY KIT KB03009

BQCkit Nitrite Determination Assay Kit is recommended for the determination of nitrite in biological samples. The assay described here measures the nitrite anion. The detection is based of spectrophotometric properties of diazonium compound (λmax= 540 nm) obtained after several steps of nitrite reaction with sulfanilamide.

1000 tests 1-100 µM 1.317 µM

50 µL Colorimetric (540 nm) 0.0082 OD/ µM

Plasma 1 h Gold standard method for the

determination of nitrites.

NITRITE/NITRATE DETERMINATION ASSAY KIT KB03010

Nitrate/Nitrite Determination Kit is recommended for the determination of nitrite and nitrate, which is a method for the detection of nitric oxide formation. The nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors, to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmax= 540 nm) obtained after nitrite reaction in several steps with sulfanilamide.

100 tests 1-100 µM 1.317 µM

50-100 µL Colorimetric (540 nm) 0.0082 OD/ µM

Plasma 2 h Allows determination of total

nitrite (nitrite and nitrate) or only nitrite

ANTIOXIDANT ENZYMES

CATALASE ACTIVITY ASSAY KIT KB03012

The kit is designed to measure catalase activity in biological samples. Catalase is an enzyme present in blood and other tissues with antioxidant activity. BQCKit Catalase Activity Assay Kit consist on a reaction giving rise to a compound that forms a complex with the chromogen. This reaction produces a purple color directly proportional to catalase activity that can be determined by means of a simple and fast spectrophotometrical measure.

100/200/500 tests 1-90 µM 7 U/ml

20 µL Colorimetric (540 nm) 0.03 OD/U/ml

Plasma, serum, tissue 40 min – 1 h Highly reproducible and

homogenates and cell enhanced sensitivity. Positive lysates control provided.

SOD ACTIVITY ASSAY KIT KB03011

The kit is designed to measure superoxide dismutase (SOD) activity in biological samples. SOD Activity Assay Kit (Colorimetric) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.

100/200/400 tests 0.01-2 U/ml Not available

20 µL Colorimetric (450 nm) 25.7 % Inhibition/U/ml

Plasma, serum, tissue 1 h Most common method

homogenates and cell based on WST-1. lysates

FLUORESCENT REDOX PROBES LINE

DHE PROBE (INTRACELLULAR ROS ASSAY) KP06002

The kit is designed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy or fluorimeter. Cell permeant reagent Dihydroethidium (DHE) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS). DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH- E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm).

250/500/1000 tests Fluorimetric Flow cytometer, Microscopy and Fluorimeter

(Ex/Em= 510/600 application

nm) Different Protocol for Adherent and Suspension

Cell cultures 60-80 min cells

DCFH-DA PROBE (INTRACELLULAR ROS ASSAY) KP06002

The kit is design ed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy or fluorimeter. ROS Assay kit, uses 2’-7’dichlorofluorescin diacetate (DCFH-DA), a cell permeant reagent fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity in the cell. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF).

Fluorimetric: ex/em Ready to use 250/500/1000 tests 485 nm/535 nm Positive control included Flow cytometer, Microscopy and Fluorimeter Cell cultures 1-4 h

application

DHR 123 PROBE (INTRACELLULAR ROS ASSAY) KP06004

The kit is designed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy or fluorimeter. Cell permeant reagent Dihydrorhodamine 123 (DHR 123) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. After cell uptake, DHR 123 is oxidized by ROS into a fluorescent compound (Rhodamine 123). Rhodamine 123 can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 500 and 536 nm, respectively. It can be also detected by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).

250/500/1000 tests Fluorimetric: ex/em Ready to use

500 nm/536 nm Flow cytometer, Microscopy and Fluorimeter

Cell cultures 60-80 min application Different Protocol for Adherent and Suspension cells

MULTIPROBE REDOX ASSAY KIT KP06002

BQCkit Multiprobe REDOX Kit contains three different redox probes (DCFH-DA probe, DHE probe, DHR 123 probe) independently packaged. The kit is designed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy or fluorimeter.

250/500/1000 tests Fluorimetric Very competitive price

Three kits in one: The best option to find the Cell cultures 60-80 min probe you need

PHENOLIC COMPOUNDS

POLYPHENOLS ASSAY KIT – FOLIN CIOCALTEU KB03006

The F-C assay has been used as a measure of total phenols in natural products, whose basic mechanism is an oxidation/reduction reaction. The FC reagent contains phosphomolybdic/ phosphotungstic acid complexes. The method relies on the transfer of electrons in alkaline medium from phenolic compounds to form a blue chromophore constituted by a phosphotungstic/phosphomolybdenum complex where the maximum absorption depends on the concentration of phenolic compounds. The method proposed by BQCkit, is a method that can be performed both in a 96-well microplate format or in a cuvette and can therefore be applied to several phenolic compounds and food products (wines, beers, infusions, juices).

400 tests 3.5-25µg/mL Standard and plate included

Very competitive price

1 ml (microassay)/ Colorimetric Protocol for microassay (2 ml) and microplate

20μL (microplate) format (96-well plate)

Foods and 10-15 min

beverages.

ANTHOCYANINS ASSAY KIT KB03015

The kit is designed for anthocyanins and anthocyanidins detection in solution (wine, berry extracts…). BQCkit Anthocyanins Assay Kit, is an easy, fast and reliable method for detecting anthocyanins and anthocyanidins in solution, like in wine or in berry extracts.

625/1250/2500 tests Colorimetric (510 Innovative product

nm) Very competitive price

20μL 15-20 min No standard curve is neccesary

Wine, berry extracts

PROANTHOCYANIDINS ASSAY KIT KB03017

The kit is designed for proanthocyanidin quantification in solution (wine, berry extracts…). BQCkit Proanthocyanidins Assay Kit is based on reaction of 4-dimethylaminocinnamaldehyde (DMAC) with procyanidins’ A cycle’s C8, in terminal units of proanthocyanidin. This reaction produces a green/blue colored compound that can be measured by spectrophotometry.

100/200/500 tests 3.5-25µg/mL Innovative product

Very competitive price 10 µL (microplate) Colorimetric Standard and plate included

Wine, berry extracts 15-30 min.

Cellular Line

MTT CELL PROLIFERATION ASSAY KIT KF03001A/B

The kit is designed to determine cellular viability. The mitochondrial activity of the cells is reflected by the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilized for homogenous measurement. Thus any increase or decrease in viable cell number can be detected by measuring formazan concentration spectrophotometrically using a plate reader at 570 nm vs 690 nm. In the BQCkit Cell Growth Determination Kit MTT- A is neccesary to remove cell medium before resuspending MTT cristals formed. In the BQCkit Cell Growth Determination Kit MTT-B, the resulting formazan crystals are directly redissolved without removing the culture medium. This kit is suitable for cells in suspension and faster, but less sensitive, than MTT Cell Proliferation Assay A.

500/2000/5000 tests End-point measurement

Very competitive price 100 µL Colorimetric (570 Two formats: MTT-A necessary to remove cell nm vs 670 nm) medium; MTT-B without removing cell medium, Cell cultures 4 h

faster, preferable for suspension cells

RESAZURIN BASED CELL VIABILITY ASSAY KIT KC04002

The kit is designed to quantify cell viability. The BQCkit Cell Blue Viability Assay Kit procures a fluorometric method for calculating the number of viable cells present in multiwell plates. It uses the indicator dye resazurin to measure the metabolic capacity of cells (an indicator of cell viability). Viable cells preserve the ability to reduce resazurin into resorufin, which is highly fluorescent.

1250/5000 tests Fluorimetric (Ex=560 Suitable for bacteria, fungi and mammalian

cells nm; Em= 590 nm) Low toxicity: continuous studies in the same cells Mammalian cell 1-4 h Very sensitive and highly reproducible

cultures, bacteria and fungi

DNA QUANTITATION KIT -HOECHST ASSAY KC04003

The kit is designed to quantify cell viability. This proliferation assay, is based on the enhanced fluorescence and shift in the emission wavelength of the fluorochrome Hoechst 33258 upon binding cellular DNA. The assay here presents, generates a linear standard curve for DNA fluorescence versus cell number. This, enables the rapid and accurate measurement of cell number involving minimal processing time, making this assay suited for cell proliferation studies.

500 tests Fluorimetric (Ex: 350 Independent of metabolic stage

nm; Em: 460 nm) Very competitive price

Cell cultures 2 h End-point measurement

Apoptosis line

CASPASE ASSAY KIT KC04005

The kit is designed to measure caspase activity in cell cultures. Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes that play essential roles in programmed cell death. Caspase activity is measured by a short peptide containing specific cleavage sequences that are recognized by the caspase and are covalently attached to a colorimetric or fluorogenic detection probe. Initiator caspases (caspase 2, 8, 9 and 10) initiate the apoptosis signal and the executioner caspases (caspase 3, 6, and 7) carry out the mass proteolysis that leads to apoptosis. BQCKIT provides 8 different kits for assaying the activity of caspases 2, 3 6 and 9, measured by a colorimetric or fluorimetric method.

COMPARISON OF THE APOPTOSIS KITS

CASPASE 2 CASPASE 3 CASPASE 6 CASPASE 9 Reference KC04005 KC04006 KC04007 KC04008 KC04009 KC04010 KC04011 KC04012 Colorimetric

Fluorimetric

Recognized VDVAD VDVAD DEVD DEVD VEID VEID LEHD LEHD Sequence Chromophore pNA AFC pNA AFC pNA AFC pNA AFC Ex= 400 Ex= 400 Ex= 400 Ex= 400 nm; nm; Em= nm; Em= nm; Em= Detection 405 nm 405 nm 405 nm 405 nm Em= 480-520 480-520 480-520 480-520 nm nm nm nm

PROTEIN ASSAY KITS Protein quantification BRADFORD PROTEIN ASSAY KIT KB03003

The kit is designed for protein quantification in biological samples. The method is based on an absorbance shift of the dye Coomasie Brilliant Blue in which under acidic conditions the red form of the dye is converted into its blue form to bind to the protein being assayed. The formation of the dye-protein complex takes about two minutes and remains stable for an hour, so the procedure is very fast and the time for trial is not limiting.

2000/4000 tests 100-1500 µg/mL 4.2 µg

1ml (microassay)/ 5ul Colorimetric (595 nm) 0.01 OD / µg

(microplate)

Plasma, serum, urine, tissue 15-20 min Faster protocol, not sensitive

homogenates and cell towards reducing agents.. lysates.

LOWRY PROTEIN QUANTIFICATION ASSAY KIT KB03004

The kit is designed for protein quantification in biological samples. The method relies on two different reactions. The first reaction (Biuret reaction) is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. The second reaction is reduction of Folin-Ciocalteu reagent giving an intense blue compound.

1200 tests 1-1500µg/mL 1.10 µg

1ml (microassay)/ 40μL Colorimetric 0.0019 OD/ µg

(microplate)

Plasma, serum, urine, 1 h Compatible substance concentrations

tissue homogenates and table provided with the protocol cell lysates.

BCA PROTEIN ASSAY KIT KB03005

The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. The principle of the BCA assay relies on the formation of a Cu2+ protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu+. The purple colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex displays a strong absorbance at 562 nm.

200/1000 tests 0.5-2000 µg/mL 0.45 µg

75 ul (microassay) / 10µL Colorimetric (562 nm) 0.0133 OD/ µg

(microplate)

Plasma, serum, urine, tissue 20-30 min Responds more uniformly to

homogenates and cell different proteins. lysates Compatible with samples that contain up to 5% surfactants.

Protein precipitation reagents

TCA DEPROTEINIZING REAGENT KB03026

The BQCkit TCA Deproteinizing kit ensures a protein removal efficiency over 99 % with very low sample dilution that includes a neutralizing solution to adjust the pH. Recommended uses for the deproteinization of samples prior to assaying low molecular weight metabolites. The precipitated proteins will remain nonfunctional.

100/200/400 samples * * Individual volume requirement :Low concentrated samples 150 µl; High

concentrated samples 100 µl

Samples with proteins Low concentrated samples 150 µl; High concentrated samples 100 µl

15-30 min Protein removal efficiency over 99%

PCA DEPROTEINIZING REAGENT KB03027

The BQCkit PCA deproteinizing kit ensures a protein removal efficiency over 99.3 % with very low sample dilution that

includes a neutralizing solution to adjust the pH. Recommended uses for the deproteinization of samples prior to assaying small molecules, glycogen, ATP, cAMP, glutathione and antioxidants. Not compatible with organic solvents, which will leave salt precipitates.

100/200/400 samples * * The number of samples refer to an individual required volume of 90 µl per

sample

Samples with proteins 90 µl

15-30 min Protein removal efficiency over 99.3%

ZINC SULPHATE DEPROTEINIZING REAGENT KB03028

The BQCkit Zinc Sulphate Deproteinizing kit ensures a protein removal efficiency over 99 % with no sample dilution and do not require pH correction. Recommended uses for the deproteinization of samples prior to assaying low molecular weight metabolites.

* The number of samples refer to an individual required volume of 400 µl per 10 /25 /50 samples * sample. The number of vials provided equals the number of sampels to be

used.

Samples with proteins 400 µl

15-30 min Protein removal efficiency over 99 %

ACETONITRILE DEPROTEINIZING REAGENT KB03029

The BQCkit Acetonitrile Deproteinizing kit ensures a protein removal efficiency over 99.3 % and do not require pH correction. Recommended uses for the deproteinization of samples prior to assaying low molecular weight metabolites.

100/200/400 samples * * The number of samples refer to an individual required volume of 500 µl per

sample

Samples with proteins 500 µl

15-30 min Protein removal efficiency over 99.3%

SULFOSALICILIC DEPROTEINIZING REAGENT KB03030

The BQCkit Sulfosalicylic acid Deproteinizing kit ensures a protein removal efficiency over 98.3 % with low sample dilution. Recommended uses for the deproteinization of samples prior to assaying low molecular weight metabolites.

100/200/400 samples * * The number of samples refer to an individual required volume of 300 µl per

sample

Samples with proteins 300 µl

15-30 min Protein removal efficiency over 98.3%

PROTEIN CONCENTRATOR KB03031

Concentration of the protein present in a sample is necessary for some laboratory protocols. It also adds the advantage of removing other potential interfering substances like salts and detergents. The BQCkit Protein concentration kit ensures the highest protein recovery and is adapted for higher and lower initial concentrations. The protein concentrates can be used for several applications, including Western blot, SDS-PAGE or 2D-gels. Do not use the concentrates for the measurement of biochemical activity, the proteins will be denatured after this protocol.

100/200/400 samples * * The number of samples refer to an individual required volume of 1 ml per

sample

Samples with proteins 1 ml

15-30 min Protein recovery adapted for higher and lowe initial concentrations,

two protocol options.

STAIN LINE

ALCIAN BLUE STAIN KIT KH07001

Alcian Blue 2.5 pH is used to detect acid mucopolysaccharides in tissue samples, that cannot be detected with the habitual method of hematoxylin – eosin. Sulphured and carboxylated mucins can be visualized. Nuclear fast red is supplied as a counterstain, to visualize cell nucleus.

125 ml / 500 ml

Nuclei can be visualized as red and mucopolysaccharides in blue

45 min

Image: Histological Mouse Gut section (40x) stained with BQCkit Alcian Blue Stain Kit

GOMORI TRICHROME STAIN KIT KH07002

Gomori trichrome stain is used to demonstrate muscle fiber and cytoplasm in red, nuclei in black and collagen containing connective tissue in blue. Weigert’s Hematoxylin and Aniline blue are included to improve staining balance. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining

125 ml / 500 ml

Nuclei can be visualized as black, muscle fiber and cytoplasm in red and collagen containing connective tissue in blue

2 h

Histological lung section from a wild type C57BL6J mouse stained with BQCkit Gomori Stain Kit.

HARRIS MODIFIED, HEMATOXYLIN SOLUTION KH07003

Hematoxylin is frequently used together with Eosin, nuclear fast red, periodic acid or alcian blue. It is also used in immunohistochemical procedures to visualize the end product. This product does not contain mercury. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.

125 ml / 500 ml

Nuclei can be visualized as blue-purple

20-30 min

Histological Brain section (20x) from a C57BL6J wildtype mouse stained with BQCkit Harris Hematoxylin Stain Kit.

MAYER’S HEMATOXYLIN AND EOSIN STAIN KIT KH07004

Hematoxylin Eosin is a common staining technique used in a wide range of tissues. Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.

125 ml / 500 ml

Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple

35-40 min

Histological Lung section (10x) from a C57BL6J wildtype mouse stained with BQCkit Mayer’s Hematoxylin-Eosin Stain Kit.

HARRIS HEMATOXYLIN AND EOSIN STAIN KIT KH07005

Hematoxylin Eosin is a common staining technique used in a wide range of tissues. Through this method, nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.

125 ml / 500 ml

Nuclei can be visualized as blue, muscle in red, connective tissue in pink and nucleic acid associated with proteins in purple

20-30 min

Histological Brain y section (10x) from a C57BL6J wildtype mouse stained with BQCkit Harris Hematoxylin-Eosin Stain Kit.

WEIGERT’S IRON HEMATOXYLIN STAIN KIT KH07006

125 ml / 500 ml

Nuclei can be visualized as black

1-2 h

Histological Gut section (10x) from a C57BL6J wildtype mouse stained with BQCkit Weigert Hematoxylin Stain Kit.

MASSON’S TRICHROME STAIN KIT KH07007

Masson trichrome stain is used to demonstrate connective tissue elements, collagen and muscle fibers. After staining, collagen and mucin will be coloured in blue, muscle fibers, cytoplasm and keratin in red and the nuclei blue/black. Sample preparation: Formalin fixed, paraffin and frozen samples can be used. After each step, rinsing with tap water and distilled water is required unless specified

125 ml / 500 ml

Collagen and mucin can be visualized as blue, muscle fibers, cytoplasm and keratin in red and the nuclei blue/black.

2 h

Histological Gut section from a C57BL6J wildtype mouse stained with BQCkit Massons trichrome stain.

MAYER’S HEMATOXYLIN SOLUTION KH07008

125 ml / 500 ml

Nuclei can be visualized as blue-purple

40 min

Histological Lung section (20x) from a C57BL6J wildtype mouse stained with BQCkit Mayers Hematoxylin Stain.

PERIODIC ACID-SCHIFF (PAS)- ALCIAN BLUE KH07010

PAS-AB technique is used to differentiate between acidic mucins as sialomucins, sulfomucin and neutral epithelial mucin. After staining, acidic mucins will be coloured blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour. Sample preparation: Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.

125 ml / 500 ml

Acidic mucins can be coloured in blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.

45 min

Histological Gut section (10x) from a C57BL6J wildtype mouse stained with BQCKit PAS Alcian Blue stain.

PERIODIC ACID-SCHIFF (PAS)- MAYER’S HEMATOXYLIN KH07011

Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Mayer’s hematoxylin is used to stain cell nuclei.

125 ml / 500 ml

Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple

60 min

Histological Gut section (40x) ) from a C57BL6J wildtype mouse stained with BQCkit PAS Mayers Hematoxylin

PERIODIC ACID-SCHIFF (PAS)- HARRIS HEMATOXYLIN KH07012

Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Harris hematoxylin is used to stain cell nuclei.

125 ml / 500 ml

Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple

60 min

Histological Gut histology (40x) from a C57BL6J wildtype mouse stained with BQCkit PAS Harris Hematoxylin.

SCHIFF REAGENT SOLUTION KH07013

Schiffs reagent is used in the staining technique PAS. Periodic Acid Schiff is used to demonstrate simple polysaccharides, neutral muco- polisaccharides, mucoproteins, and glicolipids. It can be used together with hematoxylin or alcian blue.

125 ml / 500 ml

Only used together with the PAS Stain

60 min

Histological Gut section (40x) from a C57BL6J wildtype mouse stained with BQCkit Schiff reagent

COMPARISON OF THE HEMATOXYLIN SOLUTIONS

HARRIS MODIFIED, WEIGERT’S MAYER’S HEMATOXYLIN PRODUCT HEMATOXYLIN HEMATOXYLIN SOLUTION SOLUTION SOLUTION

Type Alum hematoxylin Alum hematoxylin Iron hematoxylin

Mordant Potassium alum Ammonium alum Ferric chloride

Time 2-5 min 5-10 min 20-30 min

Preparation Ready-to-use Ready-to-use Mix before use

Color Blue Blue Dark-blue

Alcohol Does not contain alcohol or Alcohol. Does not contain Components Mercuric oxide mercury mercury

Harris Modified, Mayer’s Hematoxylin Hematoxylin Solution Alum Solution Alum hematoxylin hematoxylin Potassium Ammonium alum 5-10 min alum 2-5 min Ready-to-use Demonstrate connective Ready-to-use Blue Does not Blue Alcohol tissue fibers when used contain alcohol or mercury Uses Mercuric oxide General with trichromic techniques. Combination with PAS and use. Demonstration of Combination with acidic Oil Red O. malignant and non staining solutions Immunoperoxidase malignant cells. techniques. Progressive or Combination with PAS. regressive Progressive or regressive

Procedure Progressive or regressive Progressive or regressive Progressive

Product list

OXIDATIVE STRESS LINE Size (96 well Reference Product Name Measure Detection format) Antioxidant Capacity DMPD Antioxidant KF01001 200 tests measurement in food and Colorimetric (553 nm) Capacity Assay Kit beverage samples Antioxidant Capacity ABTS Antioxidant KF01002 250 tests) measurement in food and Colorimetric (734 nm) Capacity Assay Kit beverage samples Antioxidant Capacity FRAP Antioxidant KF01003 500 tests measurement in biological Colorimetric (593 nm) Capacity Assay Kit samples (plasma) Antioxidant Capacity Fast FRAP Antioxidant 100 – 200 -500 KF01006 measurement in biological Colorimetric (593 nm) Capacity Assay Kit tests samples (plasma) Antioxidant Capacity CUPRAC Antioxidant KF01005 250 tests measurement in biological Colorimetric (450 nm) Capacity Assay Kit samples (plasma) Antioxidant Capacity Fluorimetric measurement in biological ORAC Antioxidant (Excitation = 485 nm; KF01004 96- 192 -960 samples, cell lysates, tissue Capacity Assay Kit Emission = 528 - 538 homogenates, food and nm ) beverage samples

Antioxidant Capacity DPPH Antioxidant KF01007 100 – 200 tests measurement in food and Colorimetric (517 nm) Capacity Assay Kit beverage samples

The kit is designed to Lipid Peroxidation KB03002 200 tests determine lipid peroxidation Colorimetric (586 nm) Assay Kit in biological fluids The kit is designed to measure Thiol Quantification free thiols and disulfides in KB03007 200 tests Colorimetric (412 nm) Assay Kit biological samples (urine, plasma, cell extracts…) The kit is designed for the Protein Carbonylation measurement of oxidized Colorimetric (335-375 KB03008 100 tests Assay Kit proteins in many kind of nm) samples.

The kit is designed for nitrite Nitrite Determination KB03009 1000 test determination in biological Colorimetric (540 nm) Assay Kit samples Nitrite/Nitrate The kit is designed for KB03010 Determination Assay 100 tests nitrite/nitrate determination in Colorimetric (540 nm) Kit biological samples The kit is designed to measure Catalase Activity KB03012 100 -200 500 tests catalase activity in biological Colorimetric (540 nm) Assay Kit samples

The kit is designed to measure KB03011 SOD Activity Assay Kit 100 -200 400 tests superoxide dismutase (SOD) Colorimetric (450 nm) activity in biological samples

The kit is designed to measure KB03016 MDA-TBARS Assay Kit 100 -200 400 tests malondialdehyde (MDA) in Colorimetric (532 nm) biological samples

PHENOLIC COMPOUNDS LINE Size (96 well Reference Product Name Measure Detection format) The kit is designed for the rapid Polyphenols Assay KB03006 400 tests determination of polyphenols Colorimetric (700 nm) Kit- Folin Ciocalteu in various samples The kit is designed for anthocyanins and Anthocyanins KB03015 625- 1250- 2500 tests anthocyanidins detection in Colorimetric (510 nm) Assay Kit solution (wine, berry extracts…) The kit is designed for Proanthocyanidins proanthocyanidin KB03017 100-200- 500 tests Colorimetric (640 nm) Assay Kit quantification in solution (wine, berry extracts…)

FLUORESCENT REDOX PROBES LINE Reference Product Name Size (96 well format) Measure Detection The kit is designed for Fluorimetric 2- reactive oxygen species DHE probe OH-E+: ex 500- (ROS) detection in cell KP06002 (Intracellular ROS 250 -500- 1000 tests 530 nm/em 590- cultures by flow assay) 620 nm; E+: ex 480 cytometer, microscopy or nm/em 576 nm fluorimeter The kit is designed for reactive oxygen species Fluorimetric DCFH-DA probe (ROS) detection in cell (Excitation = 495 KP06003 (Intracellular ROS 250 -500- 1000 tests cultures by flow nm; Emission = assay) cytometer, microscopy or 529 nm ) fluorimeter The kit is designed for reactive oxygen species Fluorimetric DHR 123 probe (ROS) detection in cell (Excitation = 500 KP06004 (Intracellular ROS 250 -500- 1000 tests cultures by flow nm; Emission = assay) cytometer, microscopy or 336 nm ) fluorimeter The kit is designed for reactive oxygen species Multiprobe REDOX (ROS) detection in cell KP06005 250 -500- 1000 tests Fluorimetric Assay Kit cultures by flow cytometer, microscopy or fluorimeter PROTEIN QUANTIFICATION Reference Product Name Size (96 well format) Measure Detection The kit is designed for Bradford Protein Colorimetric (595 KB03003 2000 - 4000 tests protein quantification in Assay Kit nm) biological samples The kit is designed for Lowry Protein Assay Colorimetric KB03004 1200 tests protein quantification in Kit (560-680 nm) biological samples The kit is designed for BCA Protein Assay protein Colorimetric (562 KB03005 200- 1000 tests Kit quantification in nm) biological samples The kit is designed for SP04001 Ponceau Solution 500 ml protein detection in Stainning Western Blot membranes

PROTEIN PRECIPITATION Reference Product Name Size Price (€) KB03026 100 tests 167 TCA -deproteinizing reagent 200 tests 275 400 tests 352 KB03027 100 tests 167 PCA Deproteinizing kit 200 tests 275 400 tests 352 KB03028 10 tests 255 Zinc Sulphate-deproteinizing reagent 25 tests 437 50 tests 635 KB03029 100 tests 167 Acetonitrile- deproteinizing reagent 200 tests 275 400 tests 354 KB03030 100 tests 167 Sulfosalicilic- deproteinizing reagent 200 tests 275 400 tests 352 KB03031 100 tests 256 Protein concentrator 200 tests 435 400 tests 636

BQCELL - CELLULAR LINE Size (96 well Reference Product Name Measure Detection format) The kit is designed to MTT Cell Proliferation Colorimetric (570 nm KF03001A 500- 2000- 5000 tests determine Assay Kit, option A vs 690 nm) cellular viability

The kit is designed to MTT Cell Proliferation Colorimetric (570 nm KF03001B 500- 2000- 5000 tests determine Assay Kit, option B vs 690 nm) cellular viability Fluorimetric KC04002-25 Resazurin based Cell The kit is designed to 25ml - 100 ml (Excitation = 560 nm; KC04002-100 Viability Assay Kit quantify cell viability Emission = 590 nm ) The kit is designed to Fluorimetric DNA Quantitation KC04003 500 tests determine (Excitation = 350 nm; Kit- Hoechst Assay cell proliferation Emission = 460 nm )

APOPTOSIS LINE Size (96 well Reference Product Name Measure Detection format) Caspase 2 The kit is designed to KC04005 Colorimetric Assay 100- 200 – 500 tests measure caspase 2 activity Colorimetric (405 nm) Kit in cell cultures Fluorimetric Caspase 2 The kit is designed to (Excitation = 400 nm; KC04006 Fluorimetric Assay 100- 200 – 500 tests measure caspase 2 activity Emission = 480-520 nm Kit in cell cultures ) Caspase 3 The kit is designed to KC04007 Colorimetric Assay 100- 200 – 500 tests measure caspase 3 activity Colorimetric (405 nm) Kit in cell cultures Fluorimetric Caspase 3 The kit is designed to (Excitation = 400 nm; KC04008 Fluorimetric Assay 100- 200 – 500 tests measure caspase 3 activity Emission = 480-520 nm Kit in cell cultures ) Caspase 6 The kit is designed to KC04009 Colorimetric Assay 100- 200 – 500 tests measure caspase 6 activity Colorimetric (405 nm) Kit in cell cultures Fluorimetric Caspase 6 The kit is designed to (Excitation = 400 nm; KC04010 Fluorimetric Assay 100- 200 – 500 tests measure caspase 6 activity Emission = 480-520 nm Kit in cell cultures ) Caspase 9 The kit is designed to KC04011 Colorimetric Assay 100- 200 – 500 tests measure caspase 9 activity Colorimetric (405 nm) Kit in cell cultures Fluorimetric Caspase 9 The kit is designed to (Excitation = 400 nm; KC04012 Fluorimetric Assay 100- 200 – 500 tests measure caspase 9 activity Emission = 480-520 nm Kit in cell cultures )

STAIN LINE Reference Product Name Size 125 ml KH07001 Alcian blue stain kit 500 ml 125 ml KH07002 Gomori trichrome stain kit 500 ml 125 ml KH07003 Harris modified. Hematoxylin solution 500 ml 125 ml KH07004 Mayer´s hematoxylin and eosin stain kit 500 ml 125 ml KH07005 Harris hematoxylin and eosin stain kit 500 ml 125 ml KH07006 Weigert´s iron hematoxylin stain kit 500 ml 125 ml KH07007 Masson´s trichrome stain kit 500 ml 125 ml KH07008 Mayer's hematoxylin solution 500 ml 125 ml KH07010 Periodic acid-schiff (PAS)-alcian blue 500 ml 125 ml KH07011 Periodic acid-schiff (PAS)-mayer´s hematoxylin 500 ml 125 ml KH07012 Periodic acid-schiff (PAS)-harris hematoxylin 500 ml 125 ml KH07013 Schiff reagent solution 500 ml