Curriculum Vitae

CURRICULUM VITAE

IRADA KHALILOVA

Phone: +99451 486 0659 (mobile)

ACADEMIC EXPERIENCE

10/2014 - to present Head of Department of Biological Sciences & Director of Center for Cell Pathology Research Khazar University, Baku, Azerbaijan

07/2007 – 08/2014 Scientist (Immunology/Biochemistry) Free Radical Research & Oxidative Stress Department of Pathology University of Otago, Christchurch, New Zealand

01/1995 - 09/2003 Senior Scientist (Immunology/biochemistry) Immunodiagnosis, platelet functions/homeostasis Centre for Biochemistry of Cellular Pathology Azerbaijan Medical University

09/1993 – 09/1995 Scientist (Immunochemistry) Immunodiagnosis of Diabetes/antobodies to insulin and its removal From the blood (pre-clinical experiments) Laboratory of Molecular Cardiology Research Institute of Cardiology, Baku, Azerbaijan

02/1988 – 09/1993 Research associate (Immunochemistry) PhD student Laboratory of Immunochemistry Research Institute of Cardiology, Baku, Azerbaijan

TEACHING EXPERIENCE

10/2014 - to present Lecture/Laboratory Instructor Khazar University, Baku, Azerbaijan Course Titles: - Introduction to Biochemistry - Biochemical Laboratory Techniques - Immunology

2009 – 2014 Laboratory Instructor Otago University, New Zealand

09/1995 – 07/2003 Lecture/Laboratory Instructor Department of Biochemistry Azerbaijan Medical University, Baku, Azerbaijan Course Titles: - Introduction to Biochemistry - Biochemical Laboratory Techniques - Immunochemistry

09/2001 – 07/2002 Lecture/Laboratory Instructor Department of Prophylactic Medical Sciences Odlar Yurdu University, Baku, Azerbaijan Course Title: - Cellular and Molecular Immunology

PROFESSIONAL MEMBERSHIPS Society of Free Radical Research – Europe (SFRR - Europe) Azerbaijan Union Allergists and Immunologists Union Allergists and Immunologists Commonwealth of Independent States (CIS)

RESEARCH INTERESTS Laboratory diagnostics, alterations of immune response, oxidative stress and inflammation

DEVELOPED & VALIDATED ASSAYS Microscale of the affine, ion exchange and gel filtration assays for isolation of myeloperoxidase from biological samples and detection of its forms ELISA for the quantitative detection of myeloperoxidase activity and protein ELISA for the quantitative detection of oxidised proteins ELISA for the quantitative detection of antibodies to insulin

LABORATORY TECHNICAL SKILLS Gel filtration, Ion-Exchange & Affinity Chromatography Gel Electrophoresis & Western Blotting Immunoelectrophoresis Immunoprecipitation ELISA Animal handling, immunization Phlebotomy (human & rabbit) Cell culture techniques Immune and Biochemical diagnostic tests Peroxidase activity assays Taurine chloramine assay NADPH oxidation assay Tethramehtylbenzidine oxidation assay Cytochrome C assay Enzyme kinetics Platelet & neutrophil isolation Protein isolation, purification & quantification

EDUCATION

03/1990 – 08/1994 Doctor of Philosophy (Ph.D.) Biological Science - Division of Allergology & Immunology Medical University, Baku, Azerbaijan Thesis title: Development of an experimental model of immunosorbent for removal of antibodies to insulin from the circulation, and the immune status in patients with diabetes.

09/1977 – 06/1983 Biologist; Teacher of Biology & Chemistry (M.Sc) Major: Biology - Division of Biophysics & Molecular Biology Azerbaijan State University, Baku Azerbaijan Thesis title: Kinetic properties of photosynthetic cells after long term light exposure.

CERTIFICATES

11/2013 Comprehensive First Aid Course Red Cross, New Zealand

12/2000 Clinical Laboratory Diagnostics Training Certificate Advanced Training Institute for Physicians, Baku, Azerbaijan

LANGUAGE SKILLS

Azerbaijani – Full professional proficiency Russian – Full professional proficiency English – Full professional proficiency Turkish – Full professional proficiency Spanish – Elementary proficiency COMPUTER SKILLS

Microsoft Office 2011 (Word, Excel, PowerPoint, Access, Outlook) Microsoft OneNote SigmaPlot Statistics Prism-GraphPad Statistics

JOURNAL ARTICLES

1. Dickerhof N, Turner R, Khalilova I, Fantino E., Sly PD, Kettle AJ. (accepted on 26th October 2016). Oxidized glutathione and uric acid as biomarkers of early cystic fibrosis lung disease. Journal of Cystic Fibrosis – Elsevier. 2. Seidel A, Parker H, Turner R, Dickerhof N, Khalilova IS, Wilbanks SM, Kettle AJ, and Jameson GNL. Uric acid and thiocyanate as competing substrates of lactoperoxidase. J Biol Chem. 2014 Jun 13. pii: jbc.M113.544957 3. Stamp LK, Turner R, Khalilova IS, Zhang M, Drake J, Forbes LV, and Kettle AJ. Myeloperoxidase and oxidation of uric acid in gout: implications for the clinical consequences of hyperuricaemia. J. Rheumatology (Oxford). June, 2014, p. 266-273. 4. Kettle AJ, Turner R, Gangell CL, Khalilova IS, Harwood DT, Chapman AL, Winterbourn CC, Sly PD. Oxidation contributes to low glutathione in the airways of children with cystic fibrosis. European Respiratory Journal. March 2014, 44:122-129. 5. Kettle AJ, Albrett AM, Chapman AL, Dickerhof N, Forbes LV, Khalilova I, Turner R. Measuring chlorine bleach in biology and medicine. Biochimica et Biophysica Acta. February 2014, vol. 1840, issue 2, p. 781-793. 6. Chapman AL, Mocatta TJ, Shiva S, Seidel A, Chen B, Khalilova I, Paumann-Page ME, Jameson GN, Winterbourn CC and Kettle AJ. Ceruloplasminin is an endogenous inhibitor of myeloperoxidase. J. Biol. Chem. 2013, 288:6465-6477. 7. Forbes LV, Paul G Furtmüller PG, Khalilova I, Turner R, Obinger C, Kettle AJ. Isoniazid as a substrate and inhibitor of myeloperoxidase: identification of amine adducts and the influence of superoxide dismutase on their formation. J. Biochemical pharmacology, 2012. 84(7): p.949- 60. 8. Stamp LK, Khalilova I, Tarr JM, Senthilmohan R, Turner R, Haigh RC, Winyard PG, Kettle AJ. Myeloperoxidase and oxidative stress in rheumatoid arthritis. J.Rheumatology (Oxford, England), 2012. 51(10): p. 1796-803. 9. Orudzhev AG, Guseĭnova EE, Khalilova IS, Dzhavadov SA. (2003). Assessment of endogenous intoxication and thrombocyte functions in beta-thalassemia. J.Clinical Laboratory Diagnostics, (3):39-41. Russia, ISSN 0869-2084. PMID:12715396 10. Khalilova IS. (2002). Estimation of the proteolytic system in blood during beta thalassemia. J.Actual Problems of Hematology and Transfusiology, pp.81-85, Azerbaijan. 11. Khalilova IS, Orujov AG, Huseynova EE, Alekperova GA. (2002). Immunobiological violations of phormennous elements of blood during beta thalassemia. Journal of Azerbaijan Biophysical Association, pp.215-223, Azerbaijan. 12. Guseinova EE, Khalilova IS, Dzhavadov SA. (2002). Proteolytic blood system in hemoglobinophaty. J. Haematology and Transfusiology, vol.47, N. 4, pp.33-35, Russia ISSN 0234-5730. 13. Khalilova IS, Jokharidze TZ, Gelfgat EB, Javadov SA. (1994). Development of experimental model of the immunosorbent for removal antibodies to insulin. J. Immunology, N.3, pp.58- 61, Russia, ISSN 0206-4952. 14. Khalilova IS, Jokharidze TZ, Javadov SA.(1993). Research on possibilities for removing antibodies to insulin from circulation. In the book: Oncology and related sciences nowadays, pp.38-39, Baku. 15. Khalilova IS, Javadov SA. (1993). Correlation between main parameters of immune status and levels of anti-insulin antibodies in patients with diabetes mellitus type I and II. In the book: Oncology and related sciences nowadays, pp.36-38, Baku. 16. Khalilova IS, Jokharidze TZ, Gelfgat EB, Javadov SA. (1993). Evaluation the immune status in patients with diabetes mellitus types I and II.Azerbaijan Medical Journal, N.7-8, pp.38-41, Baku, ISSN 0005- 2523. 17. Khalilova IS, Gelfgat EB, Jokharidze TZ, Javadov SA. (1993). Main parameters of the immune system and levels of insulin antibodies during diabetes mellitus. J. Immunology, N.3, pp.46-48, Russia, ISSN 0206-4952. 18. Khalilova IS, Jokharidze TZ, Gelfgat EB, Javadov SA. (1992). Immunosorbent preparation for the anti-insulin antibodies removal from the insulin-resistant diabetic patients’ plasma. Azerbaijan Medical Journal, N.7-8, pp.12-16, Baku ISSN 0005-2523. 19. Khalilova IS, Jokharidze TZ, Javadov SA. (1991). Elaboration of experimental model of immunosorbent for removing antibodies to insulin from circulation. In Publications of Transactions of the National Academy of Sciences of (biological sciences series), pp.5-8, Baku.

CONFERENCE PROCEEDINGS 1. Baghirov M., Khalilova IS. Sustainable development research in biology. Abstracts of the 18th Symposium for Biology Students in Europe. Greece 2015. 2. Халилова И.C. (2015). Миелопероксидаза и оксидативный стресс при подагре. Международная Конференция посвященная проблеме антибиотикорезистентности и профилактике внутрибольничных инфекций Национальной медицинской академией последипломного образования им. П. Шупика, Киев, стр. 77. 3. Stamp LK, Khalilova I, Turner R, Chapman P, O’Donnell J, Kettle A. Oxidative Stress from Use of Allopurinol - Is There a Reason for Patients with Gout to Take Vitamin C? 2014 American College of Rheumatology/Association of Rheumatology Health professionals, 2014 ACR/ARHP ANNUAL MEETING. 4. Stamp LK, Khalilova I, Zhang M, Turner R, Kettle A. Oxidation of urate to allantoin by myeloperoxidase In gout. Arthritis & Rheumatism, Vol.64, November 9-14, 2012. Suppl 10:1903, DOI: 10.1002/art.39635. 5. Kettle AJ, Turner R, Gandell CL, Khalilova I, Harwood DT, Chapman AL, Winterbourn CC, Sly DT. Oxidation of glutathione in the airways of young children with cystic fibrosis. B33, Oxidants, 2011.183.1_MeetingAbstracts.A2793. 6. Stamp LK, Khalilova I, Kettle A. (2010). Acute Gout Results In Increased Plasma Myeloperoxidase: A Potential Mechanism for Increase Cardiovascular Risk? Supplement, Abstracts of the American College of Rheumatology/Association of Rheumatology Health Professionals Annual Scientific Meeting Atlanta, Georgia. DOI: 10.1002/art.28638 7. Khalilova I, Mocatta T, Marshall C, McClean D, Winterbourn C, Kettle A. (2009). Detection of active and inactive myeloperoxidase in plasma, 5th Joint Meeting of the Societies for Free Radical Research Australasia and Japan Mutagenesis and Experimental Pathology Society of Australia, Sydney, p.147-148. 8. Gurbanova MM, Khalilova IS. (2001). Comparative research of the basic parameters of cellular and humoral immunity in patients with the posttraumatic haemorrhages eyes during Perftoran treatment. Proceedings of the International Scientific Conference “Family Health in XXI Century”, pp.42-43, Perm (Russia)-Dubai (UAE). 9. Khalilova IS, Huseynova EE. (2002). Study of the thrombocytes and homeostasis during beta thalassemia. Proceedings of the International Scientific Conference “Family Health in XXI Century,” pp.149-150, Perm (Russia)-Dubai (UAE). 10. Orujov AG, Huseynova EE, Khalilova IS. (2001). The endogenous intoxication state during beta thalassemia. Proceedings of the I Conference of Azerbaijan Society of Biochemists and Molecular Biologists, pp.8-9, Baku. 11. Huseynova EE, Khalilova IS. (2000). Interrelation of the protective systems and endogenous Oxidases and Redox Regulation of Lung Disease, 2011: A2793, 10.1164/ajrccm- conference. intoxication in patients with hemoglobinopathy.Proceedings of the Scientific Conference Dedicated to 100-years Anniversary of A.S.Hasanov, p.62, Baku. 12. Javadov SA, Orujov AH, Khalilova IS. (2000). Study of platelet proteins and antibodies during beta thalassemia. Proceedings of the Scientific Conference Dedicated to 100-years Anniversary of A.S.Hasanov, p.57, Baku. 13. Huseynova EE, Khalilova IS, Javadov SA. (2000). The endogenous intoxication state and sorption ability of erythrocytes in children’s blood during sickle cell anaemia. Proceeding of the VI Congress of International Society o Immunorehabilitation, J.on Immunorehabilitation, v.2, N.2, p.48, Israel, ISSN 1562-3629. 14. Orujov AH, Khalilova IS. (1999). Definition of different forms of immune complexes and platelet antibodies in children’s blood with beta thalassemia.Proceedings of the II Congress of Azerbaijan Society of Physiologists, p.151, Baku. 15. Huseynova EE, Khalilova IS. (1999). Main parameters of humoral immunity in children with beta thalassemia. Proceedings of the I National Congress of Azerbaijan Society of Allergology, Immunology and Immunorehabilitation, pp.130-131, Baku. 16. Orujov AH, Khalilova IS. (1999). Antibodies to thrombocytes in children with beta thalassemia. Proceedings of the I National Congress of Azerbaijan Society of Allergology, Immunology and Immunorehabilitation, p.214, Baku. 17. Orujov AH, Khalilova IS, Javadov SA. (1999). The content of immune complexes and platelet antibodies in patients with beta thalassemia. Proceedings of the Joint Meeting of Pediatric Assembly, European Respiratory Society and European Society of Pediatric Allergy and Clinical Immunology, Allergy: European Journal of Allergy & Clinical Immunology, Suppl. 51, v.54, p.1, Berlin, Germany, ISSN 0105-4538. 18. Orujov AH, Khalilova IS, Javadov SA. (1999). Study of humoral immunity in children with beta thalassemia. Proceedings of the V Congress of International Society on Immunorehabilitation In: International J.on Immunorehabilitation, N.12, p.131, Spain, ISSN 1562-3629. 19. Javadov SA, Khalilova IS. (1998). Heart performance and immunological parameters of blood in patients with diabetic cardiomyopathy. Proceedings of the VII International Symposium on Insulin Receptors and Insulin Action: Molecular and Clinical Aspects, p.217, Jerusalem, Israel. 20. Khalilova IS, Jokharidze TZ, Javadov SA. (1996). Content of minor, medium and large immune complexes, including immune globulins A, M, G classes in children with homozygous type of beta thalassemia. Proceedings of the III Congress of Biochemists of Azerbaijan, Baku 21. Khalilova IS. (1995). An experimental model approbation of immune sorbents based on CNBr-sepharose. Proceedings of the Scientific Conference of Azerbaijan Medical University, Baku. 22. Javadov SA, Khalilova IS. (1994) Investigation of correlation between main parameters of immune status and levels of insulin antibodies in patients with I and II types of diabetes mellitus. Proceedings of the Annual Conference of Azerbaijan Medical University, pp.145- 146. 23. Khalilova IS, Javadov SA. (1994) Study of possibility for the insulin antibodies removal from circulation. Proceedings of the Annual Conference of Azerbaijan Medical University, pp.146-147. 24. Gelfgat EB, Jokharidze TZ, Khalilova IS, Javadov SA. (1990). Comparative studies of binding capability of sepharose and cellulose while preparing immune sorbents for removing antibodies to insulin. Proceedings of the I Conference of Azerbaijan Biochemical Society, p.51.

POSTER PRESENTATIONS 1. Khalilova IS, Dickerhof N, Mocatta T, Kettle AJ. Pro-myeloperoxidase is present in circulation and a potential participant in cardiovascular disease. Redox Conference of the Queenstown Molecular Biology Meeting Society. Queenstown, New Zealand, August 2013. 2. Khalilova IS, Dickerhof N, Mocatta T, Kettle AJ. Detection of pro-myeloperoxidase in circulation. Oxygen Theme Meeting, University of Otago, Dunedin, New Zealand, October 2012. 3. Khalilova I, Chambers ST, Murdoch DR, Kettle AJ. Measurement of myeloperoxidase in urine for the detection of urinary tract infections. 19th Annual Conference of the Societies for Free Radical Research Australasia, Akaroa, New Zealand, November, 2010. 4. Khalilova I, Mocatta T, Marshall C, McClean D, Winterbourn C, Kettle A. Detection of active and inactive myeloperoxidase in plasma. 5th Joint Meeting of the Societies for Free Radical Research Australasia and Japan Mutagenesis and Experimental Pathology Sociaty of Australia, Sydney, p.147-148, December, 2009.