AC Royal Jelly Extract Moisturizing + Conditioning + Anti-Aging

Tomorrow’s Vision… Today!® AC Royal Jelly Extract

Technical Information:

Product Code: 20478

INCI Name: Butylene Glycol & 10-Hydroxydecanoic Acid & Sebacic Acid & 1,10-Decanediol

INCI Status: Conforms

Suggested Use Level: 1.0 – 10.0%

Suggested Applications: Moisturizing, Conditioning, Anti-Aging Royal Jelly

• Secretion of worker ’ salivary glands

• Serves as food for all larvae during first 3 days of life o Drones: male bees o Workers: sterile female bees o Queens: fertile female bees

• Queen bees continue to be fed the jelly beyond the third day o How “royal” jelly got its name

• Royal jelly is packed with amino acids, fatty acids, , and minerals allowing queen bees to grow larger and live longer

• Acts as an antibiotic, immunizing bees to illness- increasing longevity Royal Jelly

• B and C content led humans to use royal jelly as an immune system and energy supplement

Vitamins Mg/100g Vitamins Mg/100g Vitamins Mg/100g 1.10 Vitamin B1 2.06 Vitamin B9 0.40 2.00 Vitamin B2 2.77 Vitamin B12 0.15 Vitamin D 0.20 Vitamin B5 52.8 Inositol 42.0 5.00 Vitamin B6 11.9 Niacin 0.40

Table 1. Typical vitamin composition of Royal Jelly Extracts.

• Vitamin A, B, & C clear free radicals from the skin • are essential for energy metabolism within cells and work to prevent oil build up Royal Jelly

When Used on Skin:

• Promotes cell and tissue repair while enhancing production1,2

• Vitamins and minerals help to keep the skin hydrated and moisturized • Essential to fighting wrinkles and appearance of aged skin

• Contains royalactin, a powerful antibacterial , which can help to repair damaged skin, clear up infections, and keep skin looking healthy2

• AC Royal Jelly Extract can be beneficial in rejuvenating our skin and keeping its healthy glow

References: 1) Koya-Miyata S, Okamoto I, Ushio S, Iwaki K, Ikeda M, Kurimoto M. Bioscience, Biotechnology, Biochemistry. 2004 Apr;68(4):767-73. 2) Park HM, Hwang E, Lee KG, Han SM, Cho Y, Kim SY. Journal of Medicinal Food. 2011 Sep;14(9):899-906. AC Royal Jelly Extract

Benefits:

 Antioxidant

 Anti-Aging

 Conditions

 Moisturizes

 Energizes AC Royal Jelly Extract

Moisturization Assay Average Moisturization Protocol 120 • Equipment: DermaLab Skin Combo

100 Untreated Control • 10 volunteers M/F

80 • 2 mg of each test material on their volar forearms

60 Base Lotion • Concentration: 2.0% Moisturization (µSiemens) Moisturization 40 • Moisture levels were improved by Experimental (2% Royal 24.08% after 24 hours and by 50.69% 20 Jelly Extract in base after 4 weeks when compared to the lotion) untreated control 0 T=0 T=24 T=1 Week T=2 Weeks T=3 Weeks T=4 Weeks

Figure 1. Moisturization Results. AC Royal Jelly Extract

Moisturization Assay

Comparative Moisturization Protocol 100 • Equipment: DermaLab Skin Combo

80 • 10 volunteers M/F Base Lotion vs. Untreated Control 60 • 2 mg of each test material on their volar forearms

40 2% Royal Jelly Extract in Base Lotion vs. • Concentration: 2.0% Untreated Control 20 Percent (%) Difference • Results indicate that AC Royal Jelly 0 2% Royal Jelly Extract Extract is capable of increasing T = 0 T = 24 T = 1 Week T = 2 WeeksT = 3 WeeksT = 4 Weeks in Base Lotion vs. moisturization when compared to Hours Base Lotion -20 both the untreated control as well as the base lotion -40

Figure 2. Comparative Moisturization Results. AC Royal Jelly Extract

TEWL Assay

Comparative Transepidermal Water Loss Protocol • Equipment: DermaLab Skin Combo 35.00% • 10 volunteers M/F 30.00% • 5 mg of each test material on their 25.00% 2% Royal Jelly Extract vs. volar forearms Untreated 20.00% Control • Concentration: 2.0% 15.00% 2% Royal Jelly • Results indicate continuous

Percent (%) (%) Percent Decrease Extract vs. Base 10.00% improvements in the barrier of the skin Lotion throughout the 4-week test period 5.00% • AC Royal Jelly Extract decreased TEWL 0.00% 11% more effectively than the base T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks lotion alone

Figure 3. Improvements in barrier function following application of the test materials after a period of 4 weeks. AC Royal Jelly Extract

High Resolution Ultrasound Skin Imaging Protocol Comparative Analysis of Skin Density • Equipment: DermaLab Skin Combo 30.00% Base Lotion vs. • 10 volunteers M/F 20.00% Untreated Control • Determine the skin density of the 10.00% subject’s volar forearms

0.00% 2% Royal Jelly Extract vs. • Concentration: 2.0% -10.00% Base Lotion • Skin density was improved by 6.16% Percent(%) Change Percent(%) -20.00% after 1 week and by 17.69% after 4 2% Royal Jelly weeks when compared to the -30.00% Extract vs. Untreated untreated control Control -40.00% T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks

Figure 4. Percent difference in skin density recordings between test materials. AC Royal Jelly Extract

ORAC Assay

60 56 Protocol 50 • Assay was conducted to assess the antioxidant capacity • AC Royal Jelly Extract and Trolox® 40 37 (positive control) were prepared in 75mM phosphate buffer 30 • Materials were prepared at three

20 different concentrations/dilutions

Antioxidant Capacity (µMTE) Capacity Antioxidant 13 • Concentration: 2.5%, 5.0%, 10.0% 10

2 1 • AC Royal Jelly Extract exhibited 0 antioxidant activity comparable to 50µM Trolox 6.25µM Trolox 10% 20478 5% 20478 2.5% 20478 50µM Trolox® AC Royal Jelly AC Royal Jelly AC Royal Jelly Extract Extract Extract

Figure 5. Antioxidant capacities. AC Royal Jelly Extract

Technical Information:

Product Code: 20478

INCI Name: Butylene Glycol & 10-Hydroxydecanoic Acid & Sebacic Acid & 1,10-Decanediol

INCI Status: Conforms

Suggested Use Level: 1.0 – 10.0%

Suggested Applications: Moisturizing, Conditioning, Anti-Aging ACTIVE CONCEPTS LLC

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