UNIVERSITY OF DEFENCE FACULTY OF MILITARY HEALTH SCIENCES

ANNUAL REPORT

2013

HRADEC KRÁLOVÉ CZECH REPUBLIC

Faculty of Military Health Sciences Hradec Králové

EDITORIAL NOTES

Dear Reader: This publication presents the main activities of the Faculty of Military Health Sciences Science of the University of Defence in Hradec Králové. The 22th Annual Report includes the principal research and educational activities of the 9 departments, 1 institute and 1 centre so that it may act as a basis for internal and external evaluation respectively. Should you require more detailed information about our Faculty, it is available on our website http://fvz.unob.cz or http://www.pmfhk.cz. In case of any suggestions or comments to our activities, do not hesitate to contact us at the undermentioned address.

Vice-Dean for Research Prof. Pavel Boštík [email protected]

Our contact address in different languages is as follows:

Univerzita obrany Brno Fakulta vojenského zdravotnictví Hradec Králové

University of Defence Brno Faculty of Military Health Sciences Hradec Králové

Universität der Verteidigung Brno Wehrmedizinische Fakultät Hradec Králové

Université de la Défense de Brno Faculté de Médecine Militaire de Hradec Králové

Universidad de Defensa Brno Facultad de Sanidad Militar Hradec Králové

Universitas Defensionis Brunensis Facultas Medicinae Militaris Greczreginensis

Třebešská 1575 500 01 Hradec Králové Czech Republic

CONTENTS

EDITORIAL NOTES ...... 3 CONTENTS ...... 5 FOREWORD ...... 7 INTRODUCTION ...... 9 HISTORY ...... 9 MAIN AIMS OF THE FACULTY IN 2013 ...... 18 STRUCTURE OF THE FMHS ...... 23 DEAN OF THE FACULTY ...... 24 DEAN OF THE FACULTY AND HIS DEPUTIES ...... 25 MEMBERS OF THE SCIENTIFIC COUNCIL ...... 26 MEMBERS OF THE ACADEMIC SENATE ...... 26 MEMBERS OF THE EDUCATION COMMISSION ...... 27 MEMBERS OF THE EDITORIAL COMMISSION ...... 28 CENTRE OF ADVANCED STUDIES ...... 29 DEPARTMENT OF COMMUNICATION AND INFORMATION SYSTEMS .. 36 DEPARTMENT OF EPIDEMIOLOGY ...... 38 DEPARTMENT OF FIELD INTERNAL MEDICINE ...... 45 DEPARTMENT OF FIELD SURGERY ...... 48 DEPARTMENT OF GENERAL AND EMERGENCY MEDICINE ...... 52 DEPARTMENT OF MILITARY HEALTH SERVICE ORGANIZATION ...... 55 DEPARTMENT OF MILITARY HYGIENE ...... 57 DEPARTMENT OF PUBLIC HEALTH ...... 59 DEPARTMENT OF RADIOBIOLOGY ...... 62 DEPARTMENT OF TOXICOLOGY ...... 65 INSTITUTE OF MOLECULAR PATHOLOGY ...... 68 VIVARIUM ...... 74 VISITORS TO THE FACULTY OF MILITARY HEALTH SCIENCES ...... 75

VISITS ABROAD ...... 75 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013 .. 81 INTERNATIONAL COOPERATION ...... 85 SCIENTIFIC AND RESEARCH ACTIVITIES ...... 91 THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FACULTY OF MILITARY HEALTH SCIENCES IN 2013 ...... 95 ARTICLES IN JOURNALS WITH IMPACT FACTOR ...... 103 ARTICLES IN OTHERS JOURNALS ...... 114 TEXTBOOKS, MONOGRAPHS, ARTICLES IN MONOGRAPHS AND PROCEEDINGS ...... 121 ABSTRACTS IN JOURNALS WITH IMPACT FACTOR ...... 124 ABSTRACTS ...... 126 PRESENTATIONS AND POSTERS ...... 132 INDEX ...... 165

FOREWORD FOREWORD

The Faculty of Military Health Sciences of the University of Defence in Hradec Kralove is a centre of medical education and research in the Czech Army with long-term history in the Czech Republic. The military medical education began in Hradec Kralove in 1951. The school was established by an order of the President of the Republic as the Military Medical Academy, and later a honorary nickname of "Jan Evangelista Purkyne" was added. Later on, the name was changed to the Military Medical Research and Training Institute and in 1988 its original name was used again. In 2004, during professionalization of the army, reorganization of military education and establishment of the University of Defence, a new phase of the faculty entitled the Faculty of Military Health Sciences began. Since 2004 our faculty has been one of the three faculties at the University of Defence. Presently, the Faculty of Military Health Sciences plays a key role in military university education with the highest scientific contribution at the university. After merging with two other originally separated military faculties, we have created a viable and developing organism emphasizing strengths of its units. Each faculty covers variety of different tasks with limited staff and resources. Our role is not only to educate and train all medical, pharmaceutical and nursing specialists and to keep scientific excellence, but also to provide a general support to the Military Medical Service which is influenced by staff cuts and restructuring policy as well. Primarily, the Faculty provides study in one accredited bachelor`s study programme (Military Paramedic), three master`s study programmes (Military General Medicine, Military Dentistry, Military Pharmacy) and eight doctoral study programmes. In spite of every year lower financial budget and personal reduction, we would like to continue and even to increase most of our activities. Our scientific production rate is the highest at the entire University of Defence. The Czech (Medical Service) field hospitals are well-known around the world and there are not so many similar good examples in our Military except military police and chemical troops. Our approach is different from the majority of other services. Our training is both long-term and intensive thus the students gain deeper knowledge and wider skills as well as awareness of military life. Education, training and research should be jointed and a pool of excellent professors, scientists and teachers should be created. But it is a long way to go. The Faculty of Military Health Sciences is an open body for mutual cooperation with scientists and teachers from all democratic countries. In spite of changing priorities in the Czech Military, we have been still dealing with specialization of the Czech Armed Forces in nuclear, biological and chemical protection and we have been engaged in many humanitarian and military deployments of military medical services abroad. Our Faculty will play the key role in this demanding process. We will

22th Annual Report, 2013 – 7 FOREWORD guarantee the research and fulfilment of training needs for medical corps, specialized forces and for some NATO countries. Nevertheless, our primary concern is to educate and train students and young physicians. This can be hardly possible without our closest partners, international workmates and friends. At present, the Faculty covers the needs of troops concerning medical professional training in all specializations, medical informatics, science and research. The Faculty represents an optimal model of education for less populous medical specialities in close cooperation with Charles University in Hradec Kralove. The Faculty has educated a lot of specialists not only at a national, but also (at least) at European level. A lot of important positions prove it. These positions have been held by former and present faculty personnel in important international institutions from the NATO Surgeon General in Europe, through a membership in various NATO, EU, United Nations Security Council, and World Health Organization boards, European Centre for Disease Prevention and Control. The Faculty provides and solves a lot of research projects, it has its own complex laboratory technologies for scientific work, above all within the sphere of life force protection against NBC agents. The scientific results are published in many respected international journals such as the Lancet. The very fact that the Faculty has survived all reforms, reorganization and other changes demonstrates its uniqueness, high educational, professional and research level. That could not be achieved without close cooperation with other scientific and educational workplaces. Not all universities can be proud of so close collegial relationship as we have with the Medical and Pharmaceutical Faculties of Charles University, the University of Hradec Kralove, the Faculty of Health Studies in Pardubice, the Faculty Hospital in Hradec Kralove and the Military Medical Agency. In 2012, the Central Military Hospital in Prague was declared the Military Faculty Hospital. This medical facility, with which we have cooperated very closely, creates a good background for military medical practice of our students. Personally, I value a cooperation with representatives of the city of Hradec Kralove, thanks to which the Faculty has an excellent reputation at city public. I am pleased that this collaboration continues. I, as a new dean, appreciate my predecessors of the faculty management that they have sustained the tradition of highly educated military doctors and pharmacists. Being elected the FMHS dean I consider the apex of my medical and military career. It is an honour to get an opportunity to contribute from such a top position to military pre-graduate and post-graduate education within next several years.

Dean of the Faculty of Military Health Sciences COL Assoc. Prof. Jiří Páral, M.D., Ph.D.

22th Annual Report, 2013 – 8 HISTORY INTRODUCTION

HISTORY

The Purkyně Military Medical Academy has been a long-term educational and scientific centre of the Czech Army Medical Service. There has been a very long history of systematic education of military medical personnel in our country. Its beginnings lie, as in many European countries, in the 18th century. Large, permanent armies were being built and the military medical service became a normal part of these armies. In 1776 the War Council of the Vienna Court issued an administrative order which definitely prohibited the employment of field surgeons in the armed forces who had not studied anatomy and who had not had their knowledge officially examined. This can be considered the beginning of organized education of military medical personnel in our country. Six-month courses were organized for field surgeons at the Garrison Hospital in Gumpendorf near Vienna. The fundamental milestone in the “Austrian“ stage was, however, in 1785 with the establishment of the Military Medical (Surgical) Academy named the Josephinum after its founder, the enlightened monarch and father of many political and social reforms, Emperor Joseph II. He saw the mission of the school as fulfilling these tasks: • education of qualified military surgeons (physicians) • creation of a learned society for research in medical science • creation of a permanent field sanitary commission for solving questions concerning combat casualty care. A number of renowned physicians of Czech origin significantly contributed to nearly 90 years of the school´s history. The foundation of the independent Czechoslovak Republic in 1918 meant at the same time the creation of a democratic army. The basic element of career military physician training was represented by the Military Medical School. Its establishment was the result of a decision by the Czechoslovak Republic government which by its resolution of 25 June 1926 defined the principles of recruiting professional medical and pharmaceutical personnel to the army. The Military Medical School provided professional training for military physicians and further qualification growth for the performance of higher command functions in the military medical service tructure. The development of the Czechoslovak Military Medical Service in our country was interrupted by the Second World War. When the army was disbanded a number of physicians and medical students participated in foreign and domestic resistance. The largest number of them were concentrated in England. The British government permitted medical students

22th Annual Report, 2013 – 9 HISTORY to complete their studies at British universities. They graduated from Oxford University. The Czechoslovak Military Hospital was created at London Hammersmith Hospital. A few courses of the Medical and Pharmaceutical Reserve Officer School were taught in Leamington and Walton-on-the-Naze where the Czechoslovak Brigade´s out-patients´ department was situated. Thus, the tradition of the Czechoslovak military medical educational system maintained its continuity. In 1945 the pre-war practice of recruiting professional personnel to the Military Medical Service was rebuilt. The Military Medical School in Prague was renowned. At the same time tendencies referring to the practice of some medical services of the world´s leading armies which required the establishment of an independent military medical university were increasing. The results of the Second World War and the growth of new knowledge in the field of medicine and especially military medicine played a significant role in this. In 1951 a new period began in the development of the Czechoslovak military medical educational system. This period has been permanently connected with Hradec Králové for 55 years. Rapid establishment of the Military Medical Academy (MMA) was possible only due to the fact that it was built on the basis of being a theoretical and clinical part of the Faculty of Medicine – a branch of Charles University established in 1945. Thanks to the reputation of its workers, a majority of whom became employees of the MMA, the school became an educational and scientific centre of the Czechoslovak Medical Service and within a short time gained a good reputation both at home and abroad.The MMA has educated a number of outstanding military medical specialists and the first steps of several contemporary top specialists of Czechoslovak medicine were connected with its existence. Beginning in 1958 and for the next 30 years the military medical system was transformed into the form of the Purkyně Military Medical Research and Postgraduate Institute. Research tasks and activities in the area of further schooling and specialization of military physicians and pharmacists became a fundamental part of its activity. The main portion of a further basic task of the school – the pregraduate training of future military physicians – was taken over by the renewed Faculty of Medicine of Charles University in Hradec Králové. The development of mutual cooperation between these two partner schools, to which the Faculty of Pharmacy of Charles University in Hradec Králové joined in 1976 as a significant guarantee of the education of military pharmacists, has become a part of the military medical system. In 1988 the school changed its name to the Purkyně Military Medical Academy which, institutionally, reflects more precisely the wide variety of its activities. In November 1989, the school entered a qualitatively new period of development. It has passed through a transformation which has basically changed some military-professional teaching programmes, the organizational

22th Annual Report, 2013 – 10 HISTORY structure of the school, personnel support, the composition of the educational staff and so on. The Academy has been included in the new university educational system and since 1993 (origin of the Czech Republic) has served as a training centre for Czech Army medical professionals. It has trained nearly 2600 military surgeons, dentists, and pharmacists till now. Some special activities have become a main part of the school´s activities. The humanitarian role of the Military Medical Service and the Military Medical Academy personnel in the present foci of conflicts in the world without doubt rank among them. As early as 1991 an independent Czechoslovak NBC battalion was sent to the Gulf. In 1994 a further tradition was established – regular operation of military medical personnel in peace-keeping missions in the territory of the former Yugoslavia. The 6th Field Hospital is known to the public for its operations abroad, first in the former Yugoslavia and later in Albania, and then in Turkey following the earthquake in that country. In 2002 members of the Czech Army Military Medical Service were employed in the ISAF mission in Afghanistan. From May to October it was the 6th Field Hospital. Then this mission was taken over by the 11th Field Hospital which completed its operations at the end of 2002. Professional training and personal acquaintance of both field hospitals personnel before their departure abroad has been traditionally carried out at the Purkyně Military Medical Academy. Some employees of the Purkyně Military Medical Academy are representatives at international non-governmental institutions and in the positions of UN and NATO experts and advisers. The highest position within the NATO Allied Command Europe Medical Service was held by Brigadier-General Assoc. Prof. Leo Klein, M.D., CSc. He remained in this position until September 2002 when he completed his period of service. Since December 2002 he has been Surgeon General of the Czech Army Medical Service. He is known to the public for his work at the Department of Field Surgery at the Purkyně Military Medical Academy and at the Surgical Department of the Teaching Hospital in Hradec Králové. COL Assoc. Prof. Roman Prymula, M.D.,CSc., Ph.D. has been elected the new Rector of the Purkyně Military Medical Academy. He officially assumed this position on October 1, 2002. The Academy continues to be a centre for integrated education and scientific research activity ensuring educational and research activities of all kinds and degrees for the training of military medical professionals. In the future its aim is to remain a modern university institution fully comparable with similar facilities and standards in other NATO countries. The year 2003 was significant with regard to different opinions on the reform of the Czech Republic Armed Forces. The initially proposed conception was reevaluated in the wake of the reform of public finances which was enforced by the Government. Therefore financial sources were

22th Annual Report, 2013 – 11 HISTORY redistributed and reduced. There were new efforts to establish an economic army structure. The Czech Republic Government Resolution no. 1154 of 12 November 2003 entitled “The Conception of the Professional Czech Republic Army Development and Mobilization of the Czech Republic Armed Forces Modified According to Financial Sources“ has become the final document respecting NATO general interests. Academy life was significantly affected by the mission of the Czech Republic Army 7th Field Hospital to Iraq. (The hospital followed with activities of the Czech Chemical Protection Contingent in Kuwait). Transport of soldiers and material began on 18 April 2003. Basra, in southern Iraq, was appointed the final destination. In September 2003 a personnel rotation was carried out and the hospital finished its activities in December 2003. Our Academy significantly supported the deployment of the 7th Field Hospital through its personnel, organizational activities, professional education and training. One of the most important preconditions of transformation of the Czech Republic Army to the fully professional system, is a reorganization of military school system. In the year 2004, merital changes were done in this area. On the basis of amalgamation of the Military School of Ground Forces in Vyškov, the Military Academy in Brno and the Purkyně Military Medical Academy in Hradec Králové there was established the University of Defence in Brno. It comprises three faculties – the Faculty of Military Technology, the Faculty of Economics and Management, the Faculty of Military Health Sciences and three independent university institutes. Act No.214/2004 of the Code makes up the legal framework of a new legal subject which at the same time identified the date of establishing the University of Defence on 1 September 2004. Brig Gen Assoc. Prof. Ing. František Vojkovský, CSc. became the Rector of the University of Defence. The University of Defence was oficially opened with a solemn inauguration on 8 October 2004. After the transformation of the Purkyně Military Medical Faculty into the Faculty of Military Health Sciences (seated still in Hradec Králové), the basic functions and tasks of the school focused on a specialized training of the Czech Army medical officers and on research work in the area of military health service have been saved. However, number of school employees was cut down. A new official name of our school is: University of Defence, Faculty of Military Health Sciences in Hradec Králové. A new dean of our school became the former rector of school COL Assoc. Prof. Roman Prymula, M.D., CSc., Ph.D., on the basis of new academic bodies´ voting. In the year 2004, Czech Republic Army officers carried out their assignments of different forms in peacekeeping missions in Iraq, Afghanistan and the Balkans. Members of our school were not missing. Specialists of the Department of Field Surgery played there a principal role. In the frame of joint operation of multinational forces in Iraq (MNF – Multinational Forces

22th Annual Report, 2013 – 12 HISTORY

Iraq) they fulfilled their tasks at special work places in British military hospital. Their assistance was highly and positively assessed. During 2005 the process of establishing the new university subject – the University of Defence continued with solving the seat and the position of the Faculty of Military Health Sciences. The Faculty of Military Health Sciences received an important position in the supreme self-governing body of the university by electing COL Assoc. Prof. Jiří Kassa, M.D., CSc. as the Head of the Academic Senate of the University of Defence on 6 October 2005. He works as the Head of the Department of Toxicology and he is a chief specialist of the Czech Republic Army for toxicology. The year 2006 was a jubilee year. The staff of the Faculty of Military Health Sciences of the University of Defence commemorated the 55th anniversary of the military medical school system in Hradec Králové and its eighty-year existence in the Czech Republic. This school is an irreplaceable centre of training and education of military health care professionals of all branches for the Army of the Czech Republic.The Faculty of Military Health Sciences of the University of Defence guarantees a good quality of the solved research tasks for the benefit of the military health service. High level of the scientific and research activity facilitated the establishment of scientific cooperation with NATO and EU partners. The extent of school activities is very wide. The clinical departments provide the general public with the health care including special therapeutic activities. Military health care experts are involved in the integrated emergency system. The preparation of personnel for humanitarian and peacekeeping missions is implemented here. The school provides medical information service, experts reports and language teaching for the Army of the Czech Republic. More information about the history and the present state of the military medical school system and the Faculty of Military Health Sciences of University of Defence is to be found in the publication “Military medical school system“, edition: Ministry of Defence, Avis, Prague 2006. In 2007, intensive activity was typical for all aspects of school life. Let´s recall the most important: The Faculty of Military Health Sciences University of Defence participated in the preparation of Czech field hospital contingents (so far three of them have been sent out), which ensure the health support of ISAF mission in the region of Kabul in Afghanistan. Some medical specialists of the Faculty were directly fulfilling the mission assignments as members of the contingent: MAJ Michal Plodr, M.D., Ph.D. worked as head doctor of the hospital, MAJ Ivo Žvák, M.D. as head doctor of operating theatres, and MAJ. Jan Psutka, M.D. worked at the department of contemporary hospitalization. The main task of the field hospital is to provide professional health care for the wounded and sick during outside combat activities, as well as for their short-time hospitalization.

22th Annual Report, 2013 – 13 HISTORY

The public show of scientific and research results is traditionally an important part of school activities. The climax was the 7th Conference of the Association of Military Doctors, Pharmacists and Veterinary Doctors of the Czech Medical Society of Jan Evangelista Purkyně in October, and the 4th Conference Disaster Medicine and Traumatological Planning in November 2007. This year´s novelty was a competition for the best scientific student´s work in doctoral study programmes. The cooperation with foreign school and scientific partner institutions went on. In this context, the November visit from the Military Medical Academy Lyon, led by its new commander General Maurice Vergos, was a remarkable event. At the end of the year, an important event in school organization happened: 10th December, COL Prof. Roman Prymula, M.D., Ph.D., was inaugurated Dean of the Faculty of Military Health Sciences. His clear election to the leading function is not only appreciation of his personal, managerial and professional qualities (it belongs to his triumph in March – obtaining the professorial diploma), but also of the stability and continuity of the place and role of the Faculty of Military Health Sciences. Significant features of the activity of the Faculty of Military Health Sciences, University of Defence, were in the year 2008 the public acknowledgements which were attributed to the Faculty eminent research specialists. Already in February, Assoc. Prof. Jiří Bajgar, M.D., D.Sc. was awarded the Prize of the Rector of the University of Defence for his research work in 2007. The Scientific Council thus appreciated his extraordinarily large publishing and lecture activities, besides un-disregardable appraisement Assoc. Prof. Bajgar gained from the American Society of Toxicology, being awarded the prestige Astra Zeneca Award. The prize winner significantly contributed to clarification of the toxic effect mechanism of organo- phosphorous compounds and to the development of new prophylactic and therapeutic means against highly toxic nerve paralytic substances. In May, the above mentioned feature of the activity of the Faculty of Military Health Sciences, University of Defence converted into a handover of two letters of appointment of new Czech Universities professors to two eminent workers of the Faculty. In renowned Prague Carolinum, the letters were accepted from the hands of the President of the Republic by LTC Assoc. Prof. Jan Österreicher, M.D., Ph.D. and Assoc. Prof. Jiří Stulík, M.D., Ph.D. The Faculty workers confirmed repeatedly their both research and organizing capabilities. They became the organizers of many traditional presentations of scientific work. Large community of epidemiologists gathered at the end of May among others to worship the memory of the nestor and military specialist in the field of epidemiology, Professor Bohumil Ticháček, M.D., D.Sc. (1924–2006) by their active participation at a conference “Ticháček´s Days of Military Epidemiologists”. Similarly, in September the Faculty substantially participated in organizing the whole Republic Conference 4th Hradec Vaccinologists Days.

22th Annual Report, 2013 – 14 HISTORY

A number of talents has been revealed by presentation of students´ research work. Periodic Faculty round of research conference of students, who work mostly as scientific and teaching staff at the the Faculty Departments, took place at the end of September. CW2 Veronika Mikusová and CW2 Pavel Novotný obtained this year’s primacy. The postgraduate programme students presented their research results immediately afterwards. Works of authors CPT Karel Šmejkal, M.D., a student of postgraduate programme Military Surgery and LT Jiří Dresler, Doctor of Pharmacy, a student of postgraduate programme Molecular Pathology, were awarded the best. The international cooperation of military medical schools has been among the traditional active forms of the school work. The visit of the delegation of the leadership of partnership school École du Service de Santé des Armées from Lyon, guided by GEN Francis Huet, School Deputy Commander, confirmed the trend of continuous cooperation. At last but not least, the conference of the Association of Military Doctors, Pharmacists and Veterinary Doctors of the Czech Medical Society of Jan Evangelista Purkyně has become repeatedly much appraised specialist forums. This year’s 8th Conference content concerned mostly Disaster Medicine, Traumatology Planning and Training. The date of the Conference, the last days of November, seemed to conclude symbolically the year of noticeable presentation and at the same time extraordinary acknowledgements of the Faculty research results. CPT. Zdeněk Šubrt, M.D., Ph.D from the Department of Field Surgery, a graduate of doctoral study programme Military Surgery, was awarded the Prize of the Mayor of the Town Hradec Králové for students´ research work in 2009. Prof. Aleš Macela, D.Sc. was awarded the Prize of the Rector of the University of Defence for scientific research in 2008, especially for excellent results in solving scientific projects in the sphere of protection against effects of extra dangerous biological agents. In September 2009 the present Dean of the Faculty COL. Prof. Roman Prymula, M.D., PhD. became on the basis of selection procedure a director of the University Hospital in Hradec Králové. The Academic Senate elected LTC Assoc. Prof. Roman Chlíbek, M.D., Ph.D a new Dean of the Faculty. The Rector of the University of Defence appointed him as dean on 15th October 2009. In the year 2010, Prof. Prymula was awarded as the first author of one of the best Elsevier’s publications in 2009 for the article: Prymula R., Siegrist C.A., Chlibek R., Zemlickova H., Vackova M., Smetana J., Lommel P., Kaliskova E., Borys D., Schuerman L.: Effect of prophylactic paracetamol administration at time of vaccination on febrile reactions and antibody responses in children: two open-label, randomised controlled trials. Lancet. 2009, 374(9698):1339-50. Extraordinary high quality of this publication was

22th Annual Report, 2013 – 15 HISTORY also confirmed by many other awards during the year 2010: 2009 Kredb’s Award for original scientific work and the best publication from the Czech Medical Association of J. E. Purkyne, 2010 prof. Karel Raska’s Award for the best scientific article published during 2009. In the year 2011, Assoc. Prof. Kamil Kuca was awarded the Prize of the Rector of the University of Defence for scientific research in 2011, especially for excellent results in solving scientific projects in the sphere of protection against chemical warfare agents. Prof Stulik´s project entitled Identification of novel Francisella tularensis targets for subunit vaccine development was supported by the Defence Threat Reduction Agency, USA for the years 2011–2014. This project is aimed at development of a subunit vaccine for tularemia. It is focused on (1) the identification of surface associated or secreted virulence factors from F. tularensis using immunoproteomic approaches (2) cloning and expression of these gene products, (3) confirming the role of the selected targets in virulence, and (4) assessing these proteins as protective antigens in animal models. The Central Military Hospital in Prague became the faculty hospital for the Faculty of Military Health Sciences in 2012. In January 2013, there was a change at the position of Vice-Dean for research. When Prof. Ing. Kamil Kuča, Ph.D. holding the position of Vice- Dean for research left the FMHS, Prof. MUDr. Pavel Boštík, Ph.D. replaced him. In February 2013, scientists from the University of Defence, the Faculty of Military Health Sciences, from the University of Hradec Kralove and the Centre for Biomedical Research had the best poster presentation at 55th Czech-Slovak Psychopharmacological Conference held in Spa Jeseník. The project, the authors of which were Hroudová J., Fišar Z., Raboch J., Korábečný J., Kuča K., was entitled "In vitro effects of acetylcholinesterase inhibitors on monoaminooxidase and NADH-dehydrogenase activity". In the beginning of May 2013 the Faculty hosted a visit of a Finnish group of CBRN experts and personnel from various branches involved in rapid response management of crises. Both sides presented their main interests during a seminar organized by our Faculty and the Finnish guests were given a tour of selected facilities. This was one of the examples of developing cooperation of European countries within the framework of the European Defence Agency. In October 2013, the Rector-Commandant of the University of Defence Brigadier General Bohuslav Přikryl decorated LTC associate professor Zuzana Kročová with the University Medal for her exceptional efforts and long-term results achieved in her research work.

22th Annual Report, 2013 – 16 HISTORY

The year 2013 was the election year for the Faculty and the Senate. After 4 years it was time for the academic faculty and students to cast a vote for the Dean. In election, which was held in June, Col. Assoc. Prof. Jiří Páral, MD, Ph.D. received the majority of votes and became the new Dean in October 2013.

22th Annual Report, 2013 – 17 MAIN AIMS OF THE FACULTY IN 2013

MAIN AIMS OF THE FACULTY IN 2013

The Faculty of Military Health Sciences (FMHS) of the University of Defence in Hradec Králové is a centre of medical education, training and research of the Army of the Czech Republic. It entirely covers the needs of the troops concerning medical professional training in all specializations, medical informatics, science and research.

1. Education The main aims of the FMHS in the field of education were as follows: to provide university-level studies in the subjects of military general medicine (6 years), stomatology, pharmacy (5 years), administration and management study (3 years), medical rescue (3 years) to provide postgraduate study for Ph.D. degree (4 years) in accredited disciplines: Epidemiology Military Hygiene Field Internal Medicine Military Radiobiology Field Surgery Molecular Pathology Infectious Biology Toxicology Preventive Medicine and Public Health Medical Microbiology Protection According to the needs of the Surgeon General of the Czech Armed Forces and the Military Medical Service Administration, the Faculty ensures specialized and lifelong education of doctors, pharmacists and other military medical service personnel in specified branches of the Act No. 95/2004 of the Code about conditions of receiving professional qualification and specialized qualification to do a medical profession as a doctor, a stomatologist, and a pharmacist. It unifies the system of their training with requirements of EU. The faculty organizes and provides the training for medical personnel in active service, doctors, nurses and other medical personnel. The faculty provides professional refresher courses for medical staff, non-medical staff and non-medical personnel of field medical units- hospital base and its units in selected up-to-date topics. It takes part in continued training of doctors and health care personnel, who are sent to missions abroad as well. Unique military know-how is attractive for people, who work out of the military health care sphere. The FMHS provides courses of advanced first aid in the field not only for Military Medical Service personnel but also for professional non- medical personnel of Military Police units, reconnaissance and special units within the frame of the Czech Armed Forces, Rapid Reaction Units of the Czech Republic Police and the others.

22th Annual Report, 2013 – 18 MAIN AIMS OF THE FACULTY IN 2013

All soldiers assigned to include into foreign missions take part in extra courses of advanced first aid. Training of emergency life support in field conditions is required in medical personnel. The courses BATLS/BARTS (Battlefield Advanced Trauma Life Support) and BARTS (Battlefield Advanced Resuscitation Techniques and Skills) for doctors and nurses or health care personnel are enlarged on the problems of NBC protection and they become a significant standard not only for the whole medical service, but also for a lot of other specialists, who take part in foreign missions. Other courses concentrate on teaching and training of comprehensive knowledge necessary for providing medical care within the frame of Disaster Medicine. The FMHS also provides other teaching and training activities determined by ”The Plan of Courses and Professional Residencies Training of the Czech Armed Forces Medical Service“ and ”Notification of Director of Personal Section of the Ministry of Defence – Teaching Activities at Military schools and Training Facilities in the Czech Republic and Abroad“. It participates in medical personnel training of medical and non-medical specializations under the methodical and professional leadership, in providing instructors for training of higher cathegories of medical personnel and in teaching instructors of lower medical specialists training.

2. Scientific and research work The FMHS of the University of Defence provides and solves research tasks for the Czech Armed Forces Medical Service. Well assembled scientific teams focus on individual research tasks using state-of-the-art technologies. Within the Faculty, complex laboratory technologies for scientific work are utilized for scientific advancements, which lead to the impromement of life force protection against NBC agents. The high scientific level and the achieved results in scientific and research activities of present teams have enabled to start scientific cooperation with foreign partners. The FMHS is the only one in the Czech Republic who provides military research within the sphere of CBRNE issues in NATO and EU. The high level capabilities and international recognition of the scientific teams form a solid base for scientific cooperation with partners in NATO countries, which is financed by the NATO and EU funds. Within the sphere of the science and research, the FMHS fulfilled strategic purposes of the Czech Armed Forces transformation by targeting the priorities of the Army (biological agents, chemical agents, military health care), furthermore it joined the appropriate institutions and organizational structures of NATO and EU countries (including drawing financial NATO and EU funds) and it gained some priority results in these critical areas. From the point of view of specialization and direction of the Czech Armed Forces, the departments of the FMHS solve medical issues of biological, chemical and radiation protection. Previous as well as contemporary scientific work within the studies focused on medical aspects of the effects of NBC agents

22th Annual Report, 2013 – 19 MAIN AIMS OF THE FACULTY IN 2013 the main focus of the “Centre of Advanced Studies“. This fully corresponds with set priorities in the field of scientific and research work of the Army of the Czech Republic. The military medical service organization and management, information systems, research activities of clinical and therapeutic preventive branches represent other important fields of scientific work. Many invitations to international symposia and conferences as well as a number of publications prove that scientific knowledge is used in education. The FMHS personnel can publish achieved results in research work, therapeutic preventive activities and in educational activities in the journal Military Medical Science Letters – the oldest military specialized journal, which has been published since 1925. As of the last year, the journal is published fully in English.Together with professional scientific and pedagogical activities there are also results in lecture and publication areas. They are a part of evaluation, which is carried out annually. The faculty is successful in keeping a good level of publication activities in journals with impact factor and in other national and foreign journals. This fact enables relatively wide training activities in accredited doctoral study programmes. Nowadays (Year 2013) the Faculty participates in 5 projects of Internal Grant Agency of the Ministry of Health, 3 projects of the Ministry of Education, Youth and Sports, 5 projects of the Grant Agency of the Czech Republic, 1 project of the Ministry of Industry and Trade, 1 project of the Ministry of the Interior of the Czech Republic, 2 projects of European structural funds and 1 foreign project (DTRA). The total sum of research grants represents the amount of 18,33 mil. Czech Crowns (approximately 907 000 USD). Scientific, research and development activities in the field of medical support include prevention, diagnosis and treatment of sick and wounded. An integral part of this work is to improve the system of medical equipment administration and supply support. Research and development is carried out at 9 departments (Epidemiology, Field Internal Medicine, Field Surgery, General and Emergency Medicine, Military Hygiene, Military Medical Service Organization, Public Health, Radiobiology, Toxicology) and in the Institute of Molecular Pathology, and the Centre of Advanced Studies. In 2013, scientific work at the faculty departments, the Institute and the Centre was focused on CBRNE protection research, prevention in hygiene and epidemiology, topical problems of field surgery and field internal medicine, topical problems of organization, management, education and information science in the Military Medical Service. The continuous accreditation for proceedings to achieve professorships for the branches of Hygiene, Preventive Medicine, Epidemiology, Medical Microbiology, Toxicology, Military Radiobiology, Field Internal Medicine,

22th Annual Report, 2013 – 20 MAIN AIMS OF THE FACULTY IN 2013

Infection Biology and Molecular Pathology and the accreditation for habilitation (associate professorship) in the branches of Hygiene, Preventive Medicine and Epidemiology, Medical Microbiology, Toxicology, Field Surgery, Military Radiobiology, Field Internal Medicine, Infection Biology and Molecular Pathology gives the evidence about the excellent level of achieved results in scientific and research activities of FMHS. In 2013, there were 12 professors (prof.), 12 associate professors (doc.), 3 doctors of science (DrSc.), 62 persons with research degrees (CSc., Ph.D.) who carried out teaching and research tasks.

3. Therapeutic activities Special therapeutic activities were provided especially at the departments of Field Internal Medicine, Field Surgery and General and Emergency Medicine. Close cooperation between these subjects and the health service establishments in the region were more and more developed. Therapeutic activities were provided, especially in the field of hematologic intensive care, traumatology, hepatobiliary surgery, and at the plastic surgery departments of internal medicine and surgery, at the Teaching Hospital.

4. International cooperation The main aims of international cooperation of the FMHS were to exchange scientific, educational and therapeutic information and to develop working contacts between military medical, medical educational and research institutions of the NATO and EU countries as well as civilian medical institutions with educational, defence research and development programmes. Residency and exchange programmes for numerous students, doctors and research workers took place at those institutions. As for study programmes, the Faculty keeps close relations with partner educational institutions above all in NATO and EU countries. Every year there are exchanges of not only students but also of pedagogical staff with the Military Medical Academy (ESSA) in Lyon in France, contacts in pedagogical sphere are kept with partner schools in Germany (Sanitätsakademie der Bundeswehr, Munich), the Military Medical Academy in Sofia, Bulgaria. In the past there were contacts with schools in Lódź (Poland) and Beograd (Serbia).

5. Expert activities The membership in work groups for coordination and cooperation of military medical research and professional training at NATO (COMEDS, BIOMEDAC, RTA/RTO) and at EDA (European Defence Agency), in work groups of government experts for the Convention on the prohibition of biological, bacteriological, and chemical weapons and their destruction in Geneva and UNO, organizing scientific conferences with international participation, and solving foreign research projects under the cooperation

22th Annual Report, 2013 – 21 MAIN AIMS OF THE FACULTY IN 2013 of the FMHS personnel are very important for presentation of international cooperation results. At the FMHS there are conditions for foreign cooperation in medical research. The priority still remains in cooperation in the frame of the Human Factors Medicine of the NATO Research and Technology Organization and its work groups (TG, WG), cooperation in research projects with other foreign scientific institutions and participation in projects of 7th EU General Programme. Our aim is to intensify international cooperation in NATO focused on scientific support of the armed forces structure.

6. Scientific and educational information services Scientific and educational information services that support the whole Medical Service of the Czech Republic Army were provided by the Department of Information and Communication Technologies. Numerous literature retrievals, courses, library and printing workshops and other information services support for students, teachers, scientists, postgraduates, doctors, nurses and other medical experts were carried out.

7. Foreign missions The FMHS performed the preparation of health personnel for humanitarian and peacekeeping missions as in the preceding years. Within last years FMHS members took part in several foreign missions.

22th Annual Report, 2013 – 22 STRUCTURE OF THE FMHS STRUCTURE OF THE FMHS

22th Annual Report, 2013 – 23 DEAN OF THE FACULTY

DEAN OF THE FACULTY

COL Assoc. Prof. Jiří PÁRAL, M.D., Ph.D. In June 2013, the academic faculty and students took part in the election for the post of the Dean in June 2013. Col. Assoc. Prof. Jiři Páral, MD, Ph.D. was elected by a majo- rity of votes and became the new Dean in October 2013. Jiří Páral was born on 10 February 1968 in Svitavy, He achieved there his secondary school qualification. He graduated from Masaryk University, Faculty of Medicine in Brno and received the university degree. After graduation, he worked for a short period for the Orthopaedic Department of the hospital in Litomyšl. He served at Pohořelice infirmary during his compulsory military service. In 1993 he began working for the Department of Surgery of the Faculty Hospital in Hradec Králové. In 2001 he was appointed a senior lecturer at the Department of War Surgery of the University of Defence, Faculty of Military Health Sciences in Hradec Králové, being later promoted to general surgery team leader and then to the department head positions. In 2008 he completed the post- graduate studies and acquired the PhD degree in military surgery. In 2012 he defended his advanced thesis to acquire assistant professor degree in military surgery. He was elected the Dean of the Faculty of Military Health Sciences of the University of Defence in 2013. He has contributed to the undergraduate teaching of surgery for the Faculty of Medicine, Charles University, Hradec Králové since 1995 and to the undergraduate and postgraduate teaching of military surgery for the University of Defence, Faculty of Military Health Sciences since 2002. His international deployment experience includes the positions of chief senior surgeon for the Czech field hospital in ISAF mission in Kabul, Afghanistan, in 2002, senior surgeon – consultant for the Shaibah, Iraq, base’s UK hospital in 2004 and senior surgeon – consultant for the KAIA base French hospital in Kabul, Afghanistan in 2011. The list of background courses includes “Leadership in Trauma Disaster Management”, Szolnok, Hungary (2002), “Battlefield Advanced Trauma Life Support Course”, Hradec Králové (2002), “American Language Course”, DLI–ELC, Lackland Air Force Base, San Antonio, Texas, USA (2003), “Training in Laparoscopic and Abdominal Surgery”, Madigan Army Medical Center, Tacoma, Washington, USA (2003), “Major Incident Medical Management and Support (MIMMS) Course”,

22th Annual Report, 2013 – 24 DEAN OF THE FACULTY AND HIS DEPUTIES

Oberammergau, Germany (2009), “Advanced Trauma Life Support Course for Doctors”, Hradec Králové (2013). Colonel Jiří Páral is the Czech Armed Forces’ chief surgeon. He has devoted most of his career to abdominal surgery, colorectal surgery and upper gastrointestinal tract surgery. The background of multinational mission deployments gives him ample experience in organ traumatology, emergency and urgent care medicine. He is the author of two and co-author of three scientific monographs, of a number of lectures presented in and beyond the Czech Republic and of articles published in national and international reviewed journals. DEAN OF THE FACULTY AND HIS DEPUTIES

Dean: CHLÍBEK Roman e-mail: [email protected] (till 14 October 2013) Dean: PÁRAL Jiří e-mail: [email protected] (since 15 October 2013) Vice-Dean for Studies: PROCHÁZKA Miroslav e-mail: [email protected] Vice-Dean BOŠTÍK Pavel e-mail: [email protected] for Research: (since 01 January 2013) Vice-Dean for External BLANAŘ Roman e-mail: [email protected] Relations and Development: Head of Logistics: TOMÁŠIK Marian e-mail: [email protected]

22th Annual Report, 2013 – 25 SCIENTIFIC COUNCIL, ACADEMIC SENATE

MEMBERS OF THE SCIENTIFIC COUNCIL

ANZENBACHER Pavel BOŠTÍK Pavel ČERVINKA Miroslav FERKO Alexander (till 30 October 2013) FILIP Stanislav FUSEK Josef HERNYCHOVÁ Lenka HLÚBIK Pavol CHLÍBEK Roman JEBAVÝ Ladislav JURENKA Božetěch KASSA Jiří (since 01 November 2013) KLEIN Leo KUČA Kamil LÁZNÍČEK Milan ÖSTERREICHER Jan PÁRAL Jiří (since 01 November 2013) PELLANT Arnošt PRYMULA Roman RYŠKA Aleš (till 30 October 2013) SLABÝ Antonín STULÍK Jiří ŠPLIŇO Miroslav ŠUBRT Zdeněk (since 01 November 2013) VALACH Ivan (till 30 October 2013) VÁVROVÁ Jiřina

MEMBERS OF THE ACADEMIC SENATE

Academic members HERNYCHOVÁ Lenka (till 22 October 2013) HORÁČEK Jan (since 23 October 2013) HRSTKA Zdeněk JAKL Martin (since 23 October 2013) JUN Daniel (Head of the senate) KUBELKOVÁ Klára (since 23 October 2013) LOCHMAN Petr (since 23 October 2013) MUSÍLEK Kamil (till 22 October 2013) PÁRAL Jiří (till 22 October 2013) PAVLÍK Vladimír (since 23 October 2013) PSUTKA Jan (till 22 October 2013) SMETANA Jan (till 22 October 2013) ŠINKOROVÁ Zuzana (Vice-chairman)

22th Annual Report, 2013 – 26 ACADEMIC SENATE, EDUCATION COMMISSION

ZÁRYBNICKÁ Lenka (since 23 October 2013) ŽĎÁROVÁ KARASOVÁ Jana (Head of the senate, till 22 October 2013)

Student members HEDÁNEK Daniel (till 22 October 2013) JANOUŠKOVÁ Karla (till 22 October 2013) KOTEK Jiří (since 23 October 2013) KUČERA Tomáš (Vice-chairman, since 23 October 2013) MÜLLER Petr (since 23 October 2013) POLCAROVÁ Petra (since 23 October 2013) PUDÍK Peter (since 23 October 2013) ŘEZÁČ David (Vice-chairman, till 22 October 2013) SKOŘEPA Pavel (till 22 October 2013) STODOLA Petr (since 23 October 2013)

MEMBERS OF THE EDUCATION COMMISSION

BRNDIAR Miroslav HRSTKA Zdeněk KONŠTACKÝ Stanislav MÍŠKOVÁ Zdena POHANKA Miroslav POTÁČ Michal PROCHÁZKA Miroslav PUDÍK Peter (Head of the education commission) ROHLENA Milan SMETANA Jan STŘÍTECKÁ Hana STULÍK Jiří ŠMÍRA Pavel ŠTĚTINA Rudolf ŠUBRT Zdeněk TICHÝ Aleš VOPLATKOVÁ Zdeňka

22th Annual Report, 2013 – 27 EDITORIAL COMMISSION

MEMBERS OF THE EDITORIAL COMMISSION

BRNDIAR Miroslav HRSTKA Zdeněk KONŠTACKÝ Stanislav POHANKA Miroslav PROCHÁZKA Miroslav POTÁČ Michal (Head of the editorial commission) PUDÍK Peter ROHLENA Milan SMETANA Jan STŘÍTECKÁ Hana STULÍK Jiří ŠMÍRA Pavel ŠTĚTINA Rudolf ŠUBRT Zdeněk TICHÝ Aleš VOPLATKOVÁ Zdeňka

22th Annual Report, 2013 – 28 CENTRE OF ADVANCED STUDIES CENTRE OF ADVANCED STUDIES

Educational and Research Staff BOŠTÍK Pavel (Head of the Institute) [email protected] JOŠT Petr [email protected] JUN Daniel (Head of the Group) [email protected] KAISLEROVÁ Lenka [email protected] KUBELKOVÁ Klára [email protected] KUČA Kamil [email protected] PEJCHAL Jaroslav (Head of the Group) [email protected] POHANKA Miroslav (Head of the Group) [email protected] SHESHKO Valeria [email protected] SCHMIDT Monika (since 01 July 2013) [email protected]

Technicians HERMAN David [email protected] HRABINOVÁ Martina [email protected] TÓTHOVÁ Iveta [email protected] ŽÁKOVÁ Jitka [email protected]

Administrative, Secretarial and Other Staff ANTONINOVÁ Jana (since 01 May 2013)

MAZUROVÁ Iva

Postgraduate Students BABKOVÁ Kateřina (since 01 October 2013)

DRTINOVÁ Lucie

GILAROVÁ Zuzana

KAISLEROVÁ Lenka (till 01 October 2013) [email protected] KRAČMAROVÁ Alžběta [email protected] KŘENKOVÁ Zuzana [email protected] VÁŇOVÁ Nela (since 01 October 2013)

Centre of Advanced Studies is an integral part of Faculty of Military Health Sciences of University of Defence, Czech Republic. The main task of the Centre is the transfer, utilization, and dissemination of advanced

22th Annual Report, 2013 – 29 CENTRE OF ADVANCED STUDIES technologies for biomedical defence research and research related to the prophylaxis and pathogenesis of dangerous infectious diseases. Research projects performed at the Centre are funded by both the Ministry of Defence and various national and international grant agencies. The biological labs of BSL2 and BSL3 category and chemical lab for the analyses of highly toxic chemicals are available for research on both highly pathogenic microorganisms and highly toxic substances. The team of the Centre utilizes and develops up-to date technologies in all three areas of interest – Biological, Chemical and Radiological. Among them are technologies for histochemistry, immunohistochemistry, laser microdissection, fluorescence microscopy, proteomic technologies including isolation and proteomic analysis of total membrane proteins and lipid-raft associated proteins, genomic technologies oriented to qRT-PCR applications, gene reporter assays, genomic manipulation of bacteria, virus identification and typization assays, technologies for the qualitative and quantitative analyses of antidotes and chemical warfare agents in biological samples using different HPLC techniques, methods for determination of oxidative stress, construction of biosensors, in vitro testing of quaternary and non-quaternary inhibitors of enzyme acetyl cholinesterase, cytotoxicity evaluation of new compunds, and finally microbiological technologies needed for screening of functional properties of bacterial mutants (proliferation assays and protective tests). CBRN programmes in 2012 follow the long-lasting research strategy of the Centre. B-agent programme involves several topics aimed at management of biological crises, modelling & risk assessment, (immuno) prophylaxis of infections, immunopathogenesis of viral diseases, such as HIV and herpetic viruses, and certain biological agents (Francisella tularensis). In addition, new projects focus on the development of prophylactic means utilizing nanotechnologies in the areas of virus induced diseases in immunosuppressed organisms (e.g. by radiation). C-agent programme is focused on development of new prophylactic and therapeutic antidotes and toxic agent scavengers utilizing the methodology of 3D in silico modelling and simulation of bio-molecular three- and fourdimensional structures and inter-molecular interactions. RN-agent programme encompassing the problems of molecular markers of ionizing radiation injury is divided into biodosimetry and diagnosis and therapy of ionizing irradiation syndromes. New international contacts have been established with several European and US military and civilian research institutions. Moreover, the broad-based collaboration under the umbrella of European Defence Agency started to be the indispensable tool for reinforcement of scientific collaboration inside the European Union. Collaboration with partners is supported by two-side or multilateral projects. During short time of its existence the Centre of Advanced Studies thus achieves all attributes of fully consolidated scientific centre collaborating with substantial number of prominent European scientific institutions.

22th Annual Report, 2013 – 30 CENTRE OF ADVANCED STUDIES

RESEARCH PROJECTS

Correlation of expression of KIR alleles in NK cells in GALT and disease progression in SIV non-human primate model of AIDS Boštík, P., Boštíková, V. Supported by the Ministry of Education, Youth and Sports, 2011–2014 (Project No.: LH11019) The HIV infection in humans leads to AIDS, a deadly disease, which targets predominantly cells of the immune system. It has recently become clear that also the innate immune cells, such as NK cells play an important role in the response to the virus, especially in the acute phase, which dictates the subsequent course of the disease. The activation and inhibition processes that are in part tied to an expression of certain variants of the Killer-Ig-like receptors (KIRs) on these cells were shown to be associated with distinct outcomes of HIV disease. The aim of this project is to characterize the expression of KIR variants on NK cells in the gut associated lymphoid tissue in SIV infected rhesus macaques (RM), the only available model of human AIDS, and their correlation to the course of AIDS in RM. In addition, monoclonal antibodies against these variants, will be developed as new reagents. These data will further our knowledge of AIDS pathogenesis and may help in the subsequent vaccine design.

Determination of apoptosis in the bioptic samples taken from the colon Kohoutová, D., Pejchal, J., Šmajs, D. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2012–2015 (Project No.: NT13413) Apoptosis of colonic epithelial cells is of a very low degree in healthy humans. Factors, which influence (increase) apoptosis, have not been satisfactory explained yet. Not only primary disease itself (inflammatory bowel disease, adenoma, carcinoma), but also luminal pH and pH of the mucosa, different expression of blood group antigens, alteration of mucosal prostaglandines and S100 proteins, microbial population, nutrition etc. may play a role in apoptosis. The project will use advanced endoscopic methods, most modern possibilities of apoptosis determination in the bioptic samples (taken during colonoscopy), direct measurement of mucosal pH and bacteriocinogenotypization.

22th Annual Report, 2013 – 31 CENTRE OF ADVANCED STUDIES

Improvement of vaccination efficacy by cholinergic anti-inflammatory pathway Pohanka, M., Hrabinová, M., Pavlík, M., Boštík, P., Kuča, K. Supported by the Ministry of Education, Youth and Sports, 2011–2014 (Project No.: LH11023) Cholinergic antiinflammatory pathway (CAP) is an important tool of regulation of innate immune responses mediated by the parasympathetic nervous system. The project is aimed at evaluating the performance of drugs affecting CAP as vaccine adjuvans. The primary hypothesis is that a suppression of CAP can potentiate the vaccination efficacy. Compound HI- 6 inhibiting blood acetylcholinesterase will be tested for control purposes in comparison to drugs triggering CAP. The project will cover the following aspects :in vitro tests of drug effects, examination of effects of tested drugs on immune system alone and co-application of tested drugs with commercially available vaccine in comparison to the effect of the vaccine alone in-vivo in small laboratory animals. Basic immunochemical parameters such as immunoglobulin isotypes M and G, interleukins 2, 4 and 6 and selected biochemical parameters will be assessed. The expected results of project will be improvement of vaccines efficacy and better characterization of CAP as target of the selected drugs.

Neurobehavioral evaluation of potential Alzhemier`s disease drugs Kassa, J., Misík, J., Kuča, K., Musílek, K., Žďárová Karasová, J. Supported by the Czech Republic Grant Agency, 2012–2015 (Project No.: GAP303/12/0611) The permanent increasing incidence of Alzheimer`s disease represents a worldwide problem that can be partly solved by introducing more effective drugs. The aim of the stuidy is to characterize the effects of newly developed acetylcholinesterase inhibitors with better pharmacodynamic features derived from the model drug 7-methoxytacrine on nervous functions and especially cognitive functions. The effects of drugs will be evaluated in laboratory rats with the deficiency of cognitive functions induced by administration of 3- chinuclidinylbenzilate. The effects of newly developed drugs will be evaluated by functional observational battery and the potency to eliminate or reduce the deficiency of cognitive functions by special neurobehavioral methods oriented on memory and learning. The effects of new drugs on nervous functions and their potency to eliminate the deficiency of cognitive functions will be compared to the effects of a model drug and standard therapeutics (tacrine, donepezil, rivastigmine). Proposed study can contribute to the increase of the effectiveness of the treatment of Alzheimer`s disease.

22th Annual Report, 2013 – 32 CENTRE OF ADVANCED STUDIES

New technologies for identification and typing of biological agents Kročová, Z., Boštík, P., Hanovcová, I., Jun, D., Macela, A. Supported by the Czech Republic Ministry of Internal Affairs, 2012–2015 (Project No.: VF20122015024) The aim of the project is to develop the methodological procedures for the isolation of bacterial and viral nucleic acids and protein and no-protein toxins from natural matrices, and the procedures for their identification and typing. In the case of bacteria and viruses are designed following the methodological procedures and specific technological and laboratory units: the acquisition and cultivation of biological agents, isolation of genome and plasmid DNA, or RNA in the case of a virus, the methodology and procedures for the preparation of samples of bacterial and viral nucleic acids from complex matrices, design of qPCR primers, probes and the reaction conditions and testing and validation of the proposed methods and procedures for the identification of biological agents. For the detection of low molecular weight toxins will be used high performance liquid chromatography coupled with tandem mass spectrometry and for detection and identification of protein toxins will be used mass spectrometric method SRM (Selected reaction monitoring).

Novel inhibitors of acetylcholinesterase derived from 7-MEOTA – potential Alzheimer´s disease drugs Kuča, K., Řípová, D. Supported by the Czech Republic Grant Agency, 2011–2015 (Project No.: GAP303/11/1907) Worldwide incidence of AD was evaluated to 35 million of people in 2009. The amount of AD patients in 2050 was estimated to 110 million. The most important factor influencing the increasing amount of AD patients is the age of population (particularly European is rapidly ageing). Thus, the AD is becoming a priority of public health care and there is demand for novel drugs suitable for its treatment.The main aim of this project consist in synthesis and evaluation of novel acetylcholinesterase (AChE) inhibitors derived from 7- MEOTA as potential AD drugs. Synthesized AChE inhibitors will be tested using experimental batery consisting of molecular modelling, biochemical evaluation of AChE inhibitors, antioxidant evaluation of novel drugs, Interactions with muscarinic/nicotinic receptors and choline transporters, in vitro prediction of blood-brain barrier penetration, determination of acute toxicity of selected AChE inhibitors, evaluation of plasmatic levels after experimental administration, evaluation of novel AChE inhibitors in CNS. Based on the appropriately-managed testing system of novel AChE inhibitors, suaitable candidate will be selected and recommended for further preclinical trial.

22th Annual Report, 2013 – 33 CENTRE OF ADVANCED STUDIES

Preparation and biological evaluation of new therapeutics against to pesticides Kuča, K., Žďárová Karasová, J., Pohanka, M., Jun, D. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2011–2014 (Project No.: NT12062) The main aim of this project is to search for new and convenient therapeutics that will be more effective in therapy of pesticide poisoning (pesticides are commonly used organophosphate inhibitors of cholinesterases). New structures will be determined by using highly sophistic methodology: molecular design. Predicted structures and their analogues will be synthesized. Their therapeutic potency will be tested in in vitro studies. The acetylcholinesterase reactivators with the highest reactivation potency will be chosen into in vivo tests. During this development process other important data such as LD50 of newly synthesized therapeutics, biochemical studies and pharmacokinetics studies (distribution of newly prepared therapeutics and their ability to penetrate through the blood-brain barrier into central nervous system) will be also replenished. If some effective candidates will be found, subsequent cooperation with foreign institutes is presumed.

The role of virus associated cellular proteins in T-lymphocyte dysfunction Boštík, P., Řehulka, P., Pejchal, J., Boštíková, V., Kročová, Z. Supported by the Czech Republic Grant Agency, 2010–2014 (Project No.: GAP304/10/1161) Herpetic viruses, such as VZV, and lentiviruses, such as HIV or SIV, are enveloped viruses, which infect CD4 T cells and cause transient (VZV) or progressive (SIV) dysregulation of T cell function. This effect is mainly indirect, as the fraction of infected cells is small, but the dysregulatory effect is observed in much larger cell population. These viruses incorporate host- derived proteins into their envelopes during the process of virus maturation and these proteins can either retain their function or engage their receptors and subsequently initiate intracellular signaling. This can be mediated by Akt- GSK3 pathway and PGE metabolism, leading to T cell dysfunction and apoptosis. This proposal utilizes state-of the art proteomic approach to Identification of host cell proteins incorporated into the SIV and VZV virions. The role of these host cell proteins will be subsequently investigated in their effects on CD4 T cell signaling cascades and can therefore lead to the elucidation of mechanisms involved in CD4 T cell dysfunction and death in such diseases as chickenpox and AIDS.

22th Annual Report, 2013 – 34 CENTRE OF ADVANCED STUDIES

Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System Boštíková, V., Smetana, J., Kaislerová, L., Boštík, P. Supported by the Roche co., 2010–2014 (Project No.: VZV) Varicella-zoster virus (VZV) is a highly infectious herpesvirus that affects up of the 90 % of human population. VZV causes chickenpox (varicella) predominantly in childhood and shingles (herpes zoster) in middle to old age people. While VZV usually causes relatively mild disease in healthy individuals, VZV still causes significant morbidity in children and adults. VZV causes life-threatening disease in immunocompromised individuals such as patients who are elderly or have HIV disease . Herpes zoster affects many elderly individuals and a major complication is prolonged severe pain or post-herpetic neuralgia (PHN), both severely debilitating. The epidemiology of VZV varies geographically. We use a novel strategy for VZV genotyping based on sequencing using DNA amplified from clinical samples. Using this method, more than 400 strains isolated in Czech Republic sorted into discrete geographically distributed genotypes. Recently we continue work on the improvement of the method and we try to find more genotypes in the individual groups. The second part of study yield important data of genetic diversity of VZV in Czech Republic, which will play an important role in further understanding of epidemiology and evolution of the virus, and may in future serve as a tool for genetic prediction of virus pathogenicity or resistance development. Previous data from several laboratories, predominantly in the US and UK, indicate a specific geographic distribution of these strains as well as their potential propensity for recombination with other wild type (wt) or vaccine strains. New pyrosequencing method using GS Junior Benchtop System of whole VZV genomes further refined the phylogenetic distinctions between SNP genotypes. The new data will bring more light to widespread surveillance in countries in which the varicella vaccine is now in use.

22th Annual Report, 2013 – 35 DEPARTMENT OF COMMUNICATION AND INFORMATION SYSTEMS DEPARTMENT OF COMMUNICATION AND INFORMATION SYSTEMS

Educational and Research Staff BLAŽEK Pavel (Head of the Group) [email protected] VOPLATKOVÁ Zdeňka [email protected] ZEDNÍČEK Jiří (Head of the Department) [email protected]

Technicians KOMÁREK Vladimír [email protected] RYDRYCH Jiří [email protected]

Administrative, Secretarial and Other Staff HRDLIČKOVÁ Lenka [email protected] VÍCHOVÁ Eva [email protected] ZIKOVÁ Jitka [email protected]

The Department of communication and information systems provides the top quality information service to ensure efficient scientific, research and teaching activities for teaching and research staff as well as under- and postgraduate students of our faculty. The Department of communication and information systems consists of the group of computer applications and the library of faculty. The group of computer applications provides the operation of the faculty network, enables access to army, specialized and public information systems and supplies the needs of the Faculty with modern information technologies. Main activity of the group is ensuring the access to INTERNET and to specialized information systems. Management of data network, central management of software, servicing as well as specialized support of users is also provided. Part of this group are also graphic services that create graphic documents and posters for presentations, make arrangements and changes of drafts for printing, make digital pictures and do other associated work. It also provides the operating and updating of the web site of the Faculty (http://www.pmfhk.cz). The printing-office of the Department of communication and information systems is able to cover reprographic and printing needs of the Faculty by its own sources in limited extent.

22th Annual Report, 2013 – 36 DEPARTMENT OF COMMUNICATION AND INFORMATION SYSTEMS

The library provides students, research and teaching staff of the Faculty of Military Health Sciences and members of the Czech Army Medical Service with scientific and information services. The main information services are provided by the library with 85 000 library units concerning medicine as well as associated branches. Information sources in the field of military medicine, emergency medicine and disaster medicine are specificity of this library. The library enables access to various information databases (WoK, ScienceDirect, SCOPUS, SpringerLink, BiblioMedica, etc.) and provides systematic help when being used. The group participates in teaching activities in the doctoral study programmes and scientific education (Ph.D.) by giving lectures in Basics of Informatics focused on retrievals, processing and publication of scientific information. It also takes part in undergraduate programme in military rescue workers.

22th Annual Report, 2013 – 37 DEPARTMENT OF EPIDEMIOLOGY DEPARTMENT OF EPIDEMIOLOGY

Educational and Research Staff BOŠTÍKOVÁ Vanda [email protected] HANOVCOVÁ Irena (Head of the Group) [email protected] CHLÍBEK Roman (Head of the Department) [email protected] MAREK Jan [email protected] PRYMULA Roman [email protected] SMETANA Jan (Head of the Group) [email protected] ŠOŠOVIČKOVÁ Renata (since 01 September 2013) [email protected] ŠPLIŇO Miroslav [email protected]

Technicians BERNARDOVÁ Marta [email protected] KOŠŤÁLOVÁ Lenka [email protected] MALÍKOVÁ Martina [email protected] ŘÍHOVÁ Iva

SOLÁROVÁ Alena [email protected] VOŽENÍLKOVÁ Kristýna [email protected]

Administrative, Secretarial and Other Staff LEGERSKÁ Kateřina [email protected]

Postgraduate Students ANTAL Zdeněk BAREKOVÁ Lucie BLECHOVÁ Zuzana (till 12 June 2013) COUFALOVÁ Monika ČEČETKOVÁ Beáta (till 01 October 2013) DÍTĚ Petr DYRHONOVÁ Markéta (since 01 October 2013) FAJFR Miroslav HEDLOVÁ Dana HOBZOVÁ Lenka (since 01 October 2013) MACHAČ Jan

22th Annual Report, 2013 – 38 DEPARTMENT OF EPIDEMIOLOGY

OLEFIRENKO Kateřina (since 01 October 2013) PEK Miroslav (till 21 August 2013) SLEHA Radek VALENTA Zbyněk (till 01 October 2013) ZELENÁ Hana (since 01 September 2013)

The Department of Epidemiology as the basic educational and research component of the Faculty of Military Health Sciences (FMHS) is divided into two groups: the epidemiology group and the microbiology, disinfection, disinsection and rodent control group. The Department of Epidemiology has fulfilled the following main tasks: It has provided undergraduate education at the FMHS and at the civilian Medical Faculty of Charles University in Hradec Králové, as well as postgraduate training and postgraduate doctoral studies. The teaching activities have been particularly aimed at general and special epidemiology with respect to the topical situation in the Czech Army and in the Czech Republic. The topics of ”Emerging and Reemerging, Infectious Diseases”, “Travel Medicine” and “Dangerous Pathogens” have also been emphasized. Doctoral study programmes (Ph.D. – epidemiology and medical microbiology) are certified by the Czech Governmental Commission. Since 1997, more than 30 students have finished their Ph.D. studies. The Department of Epidemiology plays an important role in education, training and consultancy related to biological threats/weapons and preventive medicine. The members of the department participate in training and education of medical and other personnel dispatched in military peacekeeping and humanitarian missions abroad. The aim is to inform them about any health risks during staying abroad, especially about prevention of infectious diseases, possibilities of vaccination or chemoprophylaxis. They also provide both consultancy service prior to the departure abroad and a practical realization of the respective measures. The Department of Epidemiology provides an epidemiological service for the Field Hospitals of the Czech Army. Research activities have concerned clinical evaluation of the new vaccines like pneumoccocal vaccines, herpes zoster vaccines, Human Papiloma Virus vaccines, rotavirus vaccines, new adjuvanted vaccines against viral hepatitis B, Lyme disease vaccines, combined hepatitis B and typhoid fever vaccines, flu vaccines, meningoccoccal B vaccines or new vaccination schedules. Members of the department are members of different Czech journals editorial boards (e. g. “Epidemiologie, mikrobiologie a imunologie” – Prof. Splino, Military Medical Science Letters – Prof. Chlibek), and they work

22th Annual Report, 2013 – 39 DEPARTMENT OF EPIDEMIOLOGY as reviewers of international journals (Vaccine, Lancet Infectious Disease). Some of them work in a number of committees and boards: Vice-chairman of Czech Immunization Committee of the Ministry of Health (Prof. Chlibek), or European Centre for Disease Control and Prevention (Prof. Prymula, Prof. Chlibek), or Central European Vaccination Awareness Group-CEVAG (Prof. Prymula, Prof. Chlibek). Prof. Splino and Ass. Prof. Bostikova had oportunity to serve as members for Advisory Board for the Investigation and Control of Influenuza and Other Epidemic Disease and Section for Control and Development of Diagnostics Laboratory Methods of CDC. Members of the department are also members of NATO working groups and advisory committees for biological threats and weapons (BIOMEDAC – Biological Medical Advisory Committee – Prof. Chlibek) and NATO- Research&Technology Organisation (Prof. Chlibek). Disinfection, disinsection and rodent control are very important parts of the medical practice in the Czech Army. This department is the only one of its kind in the Czech Army for the assessment of the antimicrobial efficacy of disinfectants. The researchers of the microbiology group have also solved questions concerning the prevention of endogenous and exogenous infections in immunocompromised patients.

RESEARCH PROJECTS

A phase III, randomized, observer-blind, placebo controlled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety and immunogenicity of GSK Biologicals’ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2-month schedule in adults aged 70 years and older Chlíbek, R., Kalíšková, E., Smetana, J., Ditě, P., Gál, P., Vokurková, D. Supported by the GlaxoSmithKline Biologicals co., 2010–2015 (Project No.: 113077 (ZOSTER-022)) A phase III, randomized, observer-blind, placebo controlled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety and immunogenicity of GSK Biologicals’ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2-month schedule in adults aged 70 years and older. Chlíbek, R., Smetana, J., Gál, P., Dítě, P., Kalíšková, E., Vokurková, D. Supported by the GSK, 2010–2015 (Project No.: 113077 (ZOSTER-022) Study ZOSTER-022 will provide data on the vaccine efficacy in prevention of herpes zoster (HZ) and Postherpetic neuralgia (PHN) compared to placebo in adults ≥ 70 YOA. The ZOSTER-022 study will enrol subjects in the age ranges 70-79 YOA and ≥ 80 YOA in a 3:1 ratio.

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A phase III, randomized, observer-blind, placebocontrolled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety, and immunogenicity of GSK Biologicals´ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2-month schedule in adults aged 50 years and older Chlíbek, R., Kalíšková, E., Smetana, J., Dítě, P., Gál, P., Vokurková, D. Supported by the GlaxoSmithKline Biologicals co., 2010–2015 (Project No.: 110390 (ZOSTER-006)) Study ZOSTER-006 will provide pivotal data on the overall efficacy in prevention of herpes zoster (HZ) in subjects ≥ 50 YOA. The primary endpoint of this study will be overall HZ vaccine efficacy (VE) across all age cohorts. To this end, ZOSTER-006 will evaluate VE of the gE/AS01B vaccine compared to placebo in reducing the risk of developing HZ in subjects ≥ 50 YOA. This study will enrol subjects in the age ranges 50-59 YOA, 60-69 YOA, 70-79 YOA and ≥ 80 YOA.

A phase 3, open label, multi-center, extension study to assess antibody persistence and response to a third dose of Novartis meningococcal B recombinant vaccine in 4-year-old children who previously participated in study V72P12E1 Prymula, R., Chlíbek, R., Jarolímek, J., Karlová, V., Říhová, J., Hrunka, S., Novák, L. Supported by the Novartis, 2012–2013 (Project No.: V72P12E2, EUDRACT No 2011-004931-30) The main aim of the study is to explore the bactericidal antinody persistence in 4-year-old children after a fourth dose boost of rMenB+OMV NZ given at 12, 18, or 24 months of age or after a two-dose catch-up schedule of rMenB+OMV NZ administered at either 12 and 14, 18 and 20, or 24 and 26 months of age in study V72P12E1. Safety objectives are to assess the safety and tolerability of a third/fifth dose boost of vaccine or two catch-up doses administered 2 months apart to naïve 4-year-old children. Immunogenicity objectives are to explore antibody persistence after a fourth dose boost of the vaccine administered at either 12, 18, or 24 months of age in toddlers who previously received a three-dose primary series of rMenB+OMV NZ as infants (at 2, 3, 4 or 2, 4, 6 months of age).

An open, phase II long term extension study to evaluate the immune responses to and safety of GSK Biologicals’ candidate herpes zoster vaccine (gE/AS01B) at Months 48, 60 and 72 post-vaccination in healthy subjects aged 60 years of age and older Chlíbek, R., Smetana, J., Kalíšková, E. Supported by the GlaxoSmithKline Biologicals co., 2011–2013 (Project No.: 114825, Zoster-024)

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The purpose of this long-term follow-up study (ZOSTER-024) is to evaluate the immune responses of subjects who previously participated in study ZOSTER-003. Long term safety of the study vaccine administered in ZOSTER-003 will also be evaluated.

Correlation of expression of KIR alleles in NK cells in GALT and disease progression in SIV non-human primate model of AIDS Boštík, P., Boštíková, V. Supported by the Ministry of Education, Youth and Sports, 2011–2014 (Project No.: LH11019) The HIV infection in humans leads to AIDS, a deadly disease, which targets predominantly cells of the immune system. It has recently become clear that also the innate immune cells, such as NK cells play an important role in the response to the virus, especially in the acute phase, which dictates the subsequent course of the disease. The activation and inhibition processes that are in part tied to an expression of certain variants of the Killer-Ig-like receptors (KIRs) on these cells were shown to be associated with distinct outcomes of HIV disease. The aim of this project is to characterize the expression of KIR variants on NK cells in the gut associated lymphoid tissue in SIV infected rhesus macaques (RM), the only available model of human AIDS, and their correlation to the course of AIDS in RM. In addition, monoclonal antibodies against these variants, will be developed as new reagents. These data will further our knowledge of AIDS pathogenesis and may help in the subsequent vaccine design.

Epidemiological observational prospective cohort study to evaluate the sero-prevalence of Bordetella pertussis in adults in the Czech Republic Chlíbek, R., Smetana, J. Supported by the GlaxoSmithKline Biologicals co., 2011–2013 (Project No.: 115790 EPI-Pertussis) Pertussis in adolescents and adults is common, endemic and epidemic and its incidence is reportedly increasing. Adolescents and adults are the groups most commonly infected with pertussis and are the major source of contagion to infants and young children. This population based study could give an evidence to define new strategies to prevent pertussis in adults and to support the need to include aP booster in adults. The main aim of the study is to determine sero-prevalence of Bordetella pertussis infections in adults in the Czech Republic. The secondary objectives are to determine the age specific sero-prevalence of high antibody titres to Bordetella pertussis in individual age cohorts: 18–29 years of age, 30–44 years of age,45–59 years of age and 60+ , to describe the differences in age, sex, history of a contact with pertussis, history of long –lasting cough, history of medication and hospitalization due to respiratory infections in previous 12

22th Annual Report, 2013 – 42 DEPARTMENT OF EPIDEMIOLOGY months, history of pertussis vaccination status and to identify the subjects with asymptomatic pertussis in previous 12 months.

New technologies for identification and typing of biological agents Kročová, Z., Boštík, P., Hanovcová, I., Jun, D., Macela, A. Supported by the Czech Republic Ministry of Internal Affairs, 2012–2015 (Project No.: VF20122015024) The aim of the project is to develop the methodological procedures for the isolation of bacterial and viral nucleic acids and protein and no-protein toxins from natural matrices, and the procedures for their identification and typing. In the case of bacteria and viruses are designed following the methodological procedures and specific technological and laboratory units: the acquisition and cultivation of biological agents, isolation of genome and plasmid DNA, or RNA in the case of a virus, the methodology and procedures for the preparation of samples of bacterial and viral nucleic acids from complex matrices, design of qPCR primers, probes and the reaction conditions and testing and validation of the proposed methods and procedures for the identification of biological agents. For the detection of low molecular weight toxins will be used high performance liquid chromatography coupled with tandem mass spectrometry and for detection and identification of protein toxins will be used mass spectrometric method SRM (Selected reaction monitoring).

The role of virus associated cellular proteins in T-lymphocyte dysfunction Boštík, P., Řehulka, P., Pejchal, J., Boštíková, V., Kročová, Z. Supported by the Czech Republic Grant Agency, 2010–2014 (Project No.: GAP304/10/1161) Herpetic viruses, such as VZV, and lentiviruses, such as HIV or SIV, are enveloped viruses, which infect CD4 T cells and cause transient (VZV) or progressive (SIV) dysregulation of T cell function. This effect is mainly indirect, as the fraction of infected cells is small, but the dysregulatory effect is observed in much larger cell population. These viruses incorporate host- derived proteins into their envelopes during the process of virus maturation and these proteins can either retain their function or engage their receptors and subsequently initiate intracellular signaling. This can be mediated by Akt- GSK3 pathway and PGE metabolism, leading to T cell dysfunction and apoptosis. This proposal utilizes state-of the art proteomic approach to Identification of host cell proteins incorporated into the SIV and VZV virions. The role of these host cell proteins will be subsequently investigated in their effects on CD4 T cell signaling cascades and can therefore lead to the elucidation of mechanisms involved in CD4 T cell dysfunction and death in such diseases as chickenpox and AIDS.

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Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System Boštíková, V., Smetana, J., Kaislerová, L., Boštík, P. Supported by the Roche co., 2010–2014 (Project No.: VZV) Varicella-zoster virus (VZV) is a highly infectious herpesvirus that affects up of the 90 % of human population. VZV causes chickenpox (varicella) predominantly in childhood and shingles (herpes zoster) in middle to old age people. While VZV usually causes relatively mild disease in healthy individuals, VZV still causes significant morbidity in children and adults. VZV causes life-threatening disease in immunocompromised individuals such as patients who are elderly or have HIV disease . Herpes zoster affects many elderly individuals and a major complication is prolonged severe pain or post- herpetic neuralgia (PHN), both severely debilitating. The epidemiology of VZV varies geographically. We use a novel strategy for VZV genotyping based on sequencing using DNA amplified from clinical samples. Using this method, more than 400 strains isolated in Czech Republic sorted into discrete geographically distributed genotypes. Recently we continue work on the improvement of the method and we try to find more genotypes in the individual groups. The second part of study yield important data of genetic diversity of VZV in Czech Republic, which will play an important role in further understanding of epidemiology and evolution of the virus, and may in future serve as a tool for genetic prediction of virus pathogenicity or resistance development. Previous data from several laboratories, predominantly in the US and UK, indicate a specific geographic distribution of these strains as well as their potential propensity for recombination with other wild type (wt) or vaccine strains. New pyrosequencing method using GS Junior Benchtop System of whole VZV genomes further refined the phylogenetic distinctions between SNP genotypes. The new data will bring more light to widespread surveillance in countries in which the varicella vaccine is now in use.

22th Annual Report, 2013 – 44 DEPARTMENT OF FIELD INTERNAL MEDICINE DEPARTMENT OF FIELD INTERNAL MEDICINE

Educational and Research Staff BRNDIAR Miroslav [email protected] HORÁČEK Jan (Head of the Group) [email protected] JAKL Martin [email protected] JEBAVÝ Ladislav (Head of the Department) [email protected] KAČEROVSKÝ Jaroslav (Prof. Emerit.) [email protected] KUPSA Tomáš [email protected] TICHÝ Miloš (Prof. Emerit.) [email protected]

Administrative, Secretarial and Other Staff MACHAČOVÁ Iva [email protected]

Postgraduate Students BROULÍKOVÁ Karolina

(since 01 October 2013) HAJŠL Martin (since 01 October 2013) JARKOVSKÝ Patrik (since 01 October 2013) KUPSA Tomáš [email protected] PRACHAŘOVÁ Šárka RUČKA David [email protected] SEDLOŇ Pavel [email protected] VOŠKA Michal (since 01 October 2013)

The Department of Field Internal Medicine focuses systematically on the specialized postgraduate level of medical studies in the branch of field internal medicine. This discipline deals with specific military problems, in particular with the problem of saving the lives of patients suffering from serious and life-threatening conditions of non-surgical character. Such injuries generally occur during mass disasters both in wartime and in peacetime. In the Department of Field Internal Medicine the development of military internal medicine follows three basic directions or areas: therapy and prevention, pedagogical and educational methods, and scientific research:

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Work in therapy and prevention is essential for military internists, because it enables us to acquire and develop good professional aptitude and experience in care for the seriously ill. The pedagogical and educational aspect of our work follows from our therapeutic/preventive activities. The Department coordinates the Branch Council of Clinical Medical Fields and the Branch Council of Postgraduate Study for Doctorates in Accredited Disciplines of Field Internal Medicine (equivalent of Ph.D.). Scientific research is the Department´s third main area of activity. Essentially, it extrapolates the results of applied clinical research into specific military conditions and into medical care under field conditions.

In 2013 the research in the Department continued in the five basic fields: Haematology – growth of stem cells – preparation for bone marrow transplantation Biochemical and electrophysiologic investigation and monitoring of acute coronary syndromes Cardiotoxicity of antitumorous therapy Diagnosis and therapy of hypercoagulative states – the monitoring of anticoagulant therapy Global quality of life in patients who have undergone the hematopoietic stem cell transplantation

Cooperation in the clinical research: 1. Haematopoietic stem cell transplantation. A role of cytokines in transplantation. Transplant-related complications and supportive care has continued. 2. A study in the application of enteral and parenteral nutrition in intensive metabolic care has continued. 3. The study of supraventricular tachycardias – electrophysiologic investigation and therapy including radiofrequency ablation has continued. 4. Participation (co-investigators) on international study IRIS – Immediate Risk-stratification Improves Survival, primary prevention of sudden cardiac death. 5. Cardiotoxicity of antitumorous therapy – the research project has continued. 6. Projects have been carried out in the field of determination and analysis of monoclonal immunoglobulins in the urine of patients with multiple myeloma.

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7. New biochemical cardiac markers (cardiac troponin T, high sensitivity CRP, brain natriuretic peptide) – clinical and laboratory evaluation has continued. 8. Analysis of transplantation activities, indications and results in the Czech Republic – National Stem Cell Transplantation Registry. 9. GvHD prophylaxis with ATG-Fresenius S in allogeneic stem cell transplantation from matched unrelated donors: a randomised phase III multicenter trial comparing a standard GvHD prophylaxis with cyclosporine A and methotrexate with additional pretransplant ATG- Fresenius S. Project-No. (Fresenius): AP-AS-21-DE, Project-No. (ZKS): S020801 Medizinische Klinik I, Universitätsklinikum Freiburg. 10. Research project: MZO 00179906 (Czech Ministry of Health), Bioindicators in Hematology and Internal Medicine. 11. A double-blind, randomized, placebo-controlled study of two different schedules of Palifermin (pre- and post chemotherapy and pre- chemotherapy only) for reduction in severity of oral mucositis in subjects with multiple myeloma (MM) receiving high dose Melphalan followed by autologous peripheral blood stem cell transplantation (PBSCT): AMGEN, Protocol no. 20050219. 12. A long-term organization development plan 1011. 13. Double blind, randomised, placebo-controlled multicentre phase III clinical study followed by open-label phase on the efficacy and tolerability of budesonide 3 mg effervescent tablet in patiens with resistant oral chronic GvHD. 14. A phase III, double-blind, randomized, placebo-controlled, multicenter clinical trial to study the safety, tolerability, efficacy, and immunogenicity of V212/heat-treated varicella-zoster virus (VZV) vaccine in recipients of autologous hematopoietic cell transplants (HCTs).

RESEARCH PROJECTS

Quality of life measurement and its influence to overall survival in hematopoietic stem cell transplantation in CZ Trněný, M., Novák, J., Válková, V., Jebavý, L., Sedláček, P. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2010–2015 (Project No.: NT11299) The aim of our project is to document quality of life in transplanted patients using standardised QOL questionnaires. Based on this monitoring, we would like to propose a supportive psychological and social program for individual institutions so that the monitoring of QOL will become an integral part of standard medical practice.

22th Annual Report, 2013 – 47 DEPARTMENT OF FIELD SURGERY DEPARTMENT OF FIELD SURGERY

Educational and Research Staff ČÁP Robert (Head of the Group) [email protected] DOBEŠ Daniel (till 31 July 2013) [email protected] KLEIN Leo (Head of the Group) [email protected] LOCHMAN Petr [email protected] PÁRAL Jiří (Head of the Department) [email protected] ŠIMEK Jan [email protected] ŠMEJKAL Karel [email protected] ŠUBRT Zdeněk [email protected]

Administrative, Secretarial and Other Staff ZAHRADNÍČKOVÁ Jana [email protected]

Postgraduate Students DOLEŽEL Radek DUŠEK Tomáš (till 01 July 2013) FIBÍR Aleš (till 01 July 2013) FRANK Martin (till 17 September 2013) KOVÁŘ Daniel OBERREITER Martin POHNÁN Radek SLANINKA Igor

Structure and main tasks of the department 1. Division of General Surgery Klein Leo – Head of the Group 2. Division of Traumatology and Burns Treatment Čáp Robert – Head of the Group

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Main tasks Undergraduate education of medical students Postgraduate training of military surgeons and other medical specialists Expertise and referential work for needs of the Czech Armed Forces Research in Military Surgery Preparation of medical health-care personnel before foreign missions of the Army of the Czech Republic At present, the Department of Field Surgery consists of two groups – the Group of General Surgery and the Group of Traumatology and Burns Treatment. Besides working at each Division of the Department of Surgery of the Teaching Hospital in Hradec Králové, the members of the Department perform both, undergraduate courses in field surgery for students of the Faculty of Military Health Science, and postgraduate training of military physicians for their specialization exams in surgery and general medicine. The Department also participates in teaching of the Battlefield Advanced Trauma Life Support (BATLS) courses, disaster medicine and the first aid courses, organized by the Faculty of Military Health Sciences for the Czech Army members. Members of the department participate in specialised NATO working groups according to their expertise. Moreover, they are consultans of the Surgeon General of the Czech Armed Forces. In the last several years, the Department has played important role in education and training of the personnel of field hospitals operating in foreign missions (Yugoslavia, Bosna-Herzegovina, Albania, Iraq, Afghanistan). Members of the Department also took part in that missions. Research and publication activities are also essential part of the Department work. Participation in a foreign mission F. Hošek – UNTS, Zagreb, Croatia, 1996 A. Ferko – International Hospital, SFOR, Shipovo, Bosnia and Hercegovina, 2001 R. Čáp – International Hospital, SFOR, Shipovo, Bosnia and Hercegovina, 2001 A. Ferko – 11th Field Hospital, ISAF, Kabul, Afghanistan, 2002 J. Páral – 11th Field Hospital, ISAF, Kabul, Afghanistan, 2002, 2011 (TSF) M. Plodr – 11th Field Hospital, ISAF, Kabul, Afghanistan, 2002 I. Žvák – 11th Field Hospital, ISAF, Kabul, Afghanistan, 2002 D. Dobeš – British Field Hospital, Op TELIC, Shaibah, Iraq, 2004 J. Páral – British Field Hospital, Op TELIC, Shaibah, Iraq, 2004 M. Plodr – British Field Hospital, Op TELIC, Shaibah, Iraq, 2004 P. Lochman – British Field Hospital, Op TELIC, Shaibah, Iraq, 2004

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M. Plodr – 1st Contingent of the Field Hospital, ISAF, Kabul, Afghanistan 2007 I. Žvák – 1st Contingent of the Field Hospital, ISAF, Kabul, Afghanistan 2007 J. Páral – Czech Field Surgical Team, International Medical Treatment Facility (Role 3) KAIA, Kabul, Afghanistan 2012 J. Šimek – Czech Field Surgical Team, International Medical Treatment Facility (Role 3) KAIA, Kabul, Afghanistan 2012 National textbooks Endovascular Treatment of Arterial Aneurysms (Ferko et al.) Handbook of Surgery (Ferko et al.) Principles of War Surgery (Klein, Ferko et al.) X-ray Atlas of Bone Fractures (Žvák et al.) Handbook of Bandages Technique (Páral) Acute Mesenteric Ischemia (Páral) International textbooks Gastrointestinal Stromal Tumors (Páral). In Aperelho Digestivo (Coelho), Editora Atheneau, Sao Paulo, Brasilia

RESEARCH PROJECTS

Parametric monitoring the quality of TME as a tool to reduce local recurrences after surgery for rectal cancer Hoch, J., Páral, J. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2012–2015 (Project No.: NT13726) The aim of the project is to establish and standardize the method of assessing the quality of surgery in the treatment of rectal cancer as a tool to reduce the incidence of local recurrence. The expected outcome will be improving preoperative staging, consistent implementation and improvement procedures TME on those sites, increasing the percentage of complete excision as a basic prerequisite for reducing the rate of local recurrence in rectal cancer.

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Quality evaluation of multimodal treatment in patients with colorectal liver metastatic disease: Mutlicentric study within Czech complex oncology centres Ryska, M., Ferko, A., Šubrt, Z. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2012–2015 (Project No.: NT13660) The aim of this project is to verify the benefits of a multidisciplinary team approach to comprehensive treatment and evaluation of results for groups of patients defined by selected parameters, compare nationwide data with those of selected comprehensive cancer centers, while respecting the customized approach, to evaluate the cost / benefit and quality of life in patients treated for colorectal cancer liver metastases.

22th Annual Report, 2013 – 51 DEPARTMENT OF GENERAL AND EMERGENCY MEDICINE DEPARTMENT OF GENERAL AND EMERGENCY MEDICINE

Educational and Research Staff ČMEDLOVÁ Pavlína (maternity leave, Head of the [email protected] Group) DUŠKOVÁ Klára (maternity leave) [email protected] GRÖGER Tomáš [email protected] KONŠTACKÝ Stanislav (Head of the Group) [email protected] KRUTIŠOVÁ Pavla (since 01 January 2013) [email protected] MATOUŠEK Radovan (Head of the Department) [email protected]

Administrative, Secretarial and Other Staff BENDOVÁ Tereza (maternity leave) [email protected] DOLEŽELOVÁ Miroslava [email protected] KRUTIŠ Jan [email protected] ŠÍSTKOVÁ Jitka [email protected]

The Department of General Medicine was established at the J. E. Purkyně Military Medical Research and Postgraduate Institute in 1983. It was formed from the former Group of Military Medical Service Organization in Peacetime which existed at the Department of Military Medical Service Organization. The reason for the foundation of the Department was the need to educate military doctors in newly established branch of General Medicine that became the basic branch for military doctors who were in charge of primary care in the Military Medical Service organizational structures. Specialization in the branch of internal medicine was insufficient and did not meet professional requirements for individual medical practice at Units and later at Garrison Dispensaries. First Specialization Exams in this new basic specialization branch were held in February 1985. 525 military doctors passed the Specialization Exam in General Medicine till 31st December 2004. Since 1997, the work at this Department has focused more on pre- hospital emergency care and teaching the First Aid and Emergency Medicine. At the same time a significant modernization and a proper subdivision of teaching premises according to the type of courses were carried out there. Now the Department is equipped with modern teaching models and simulators for teaching pre-hospital care, including the possibility of interactive teaching aids. Current innovations of medical material and

22th Annual Report, 2013 – 52 DEPARTMENT OF GENERAL AND EMERGENCY MEDICINE equipment are applied in teaching process. The extension of teaching activities in this new field called for changes in table posts at the Department. In 2001, the Healthcare Education and Training Group was established and other workers were engaged to teach the first aid. Since 2003, regarding the extension of teaching, the Department has had a new name – the Department of General and Emergency Medicine. The Department of General and Emergency Medicine is the main department providing military-professional training in the subject called Military Medical Service Organization in Peacetime for students of the Master´s Study Programme in branches of General Medicine and Military Pharmacy, and for students of the Bachelor´s Study Programme in the branch of Military Medical Management and in various types of training and courses. It also provides further education for military doctors, pharmacists and other personnel of the Military Medical Service through refresher courses and specialization courses. Until 2005, the Department was the leading department focused on specialization training of military doctors served in the Czech Army Medical Service. It organizes pre-graduate courses in emergency medicine in Master´s Study Programme, but especially post-graduate education of doctors, health care workers and nurses. The Department of General and Emergency Medicine collaborates with the Institute of Postgraduate Medical Education in Prague, the Chamber of Medicine, professional medical societies and associations in postgraduate training and specialized activities. It participates in establishing standards for special therapeutic care. The subject called Disaster Medicine makes students acquainted with principles of emergency medicine and operation of individual parts of integrated rescue system in conditions of serious accidents, natural disasters and catastrophes. In connection with this training, the Department provides its participants with knowledge and experience of the operation of the Military Medical Service institutions and facilities in crisis, in combat or other extraordinary situations. It applies the knowledge of military and military-professional subjects into specific conditions of operation of the Military Medical Service respecting both military principles and requirements as well as the principles of humanity, law and especially Geneva Conventions. The main mission of the Department is education and training of medical officers in casualty medical care in both combat and disaster situations. For this purpose, the principles and procedures of emergency care in field conditions are taught at the Department through BATLS/BARTS (Battlefield Advanced Trauma Life Support/Battlefield Advanced Resuscitation Techniques and Skills) courses. In the same area, the Department participates in training of medical personnel before their departure to foreign missions. The next important mission od the department is education and training of non-medical personnel in first-aid care. The most of the soldiers are

22th Annual Report, 2013 – 53 DEPARTMENT OF GENERAL AND EMERGENCY MEDICINE trained in the Battlefield First-Aid Courses, some of them are trained in consequential Combat Life Saver Courses. This course lasts 2 weeks and offers a lot of useful knowledge and skills, e. g. control life-threatening external hemorrhage, airway management and decompress the chest. The graduates of this course must be able to give first aid to casualty with very realistic looking injuries prepared by proffessional masker at the end of the course. The Department is a co-ordinating centre of scientific work in the branch of Military Medical Service Organization in Peacetime, Social Medicine, Emergency Medicine and Disaster Medicine. It participates in increasing the quality of organisational structure of medical units, formations and facilities. It elaborates their operation procedures and principles of their management in peacetime as well as in emergency situations. The Department provides expert activities and elaborates data and proposals from these areas for concept-making bodies of the Czech Army Medical Service. The Department analyzes NATO regulations and directives and recommends their introduction in practice as well as in teaching process. It provides consultations for field leading officers of the Military Medical Service. The Department cooperates with civilian institutions, namely, with the bodies of the Ministry of Health of the Czech Republic in the issues concerning the cooperation between civilian and military medical service in extraordinary situations. It ensures publication activities focused on educational work requirements and on presenting scientific information. The Department is in charge of the education of talented students within the framework of students´ scientific and professional activities. It participates in the solution of assignments within the organizational structure of the military health care in peacetime. It is the consultation and expert workplace in the branch of General and Emergency Medicine for the Army of the Czech Republic.

22th Annual Report, 2013 – 54 DEPARTMENT OF MILITARY HEALTH SERVICE ORGANIZATION DEPARTMENT OF MILITARY HEALTH SERVICE ORGANIZATION

Educational and Research Staff BLANAŘ Roman [email protected] HUMLÍČEK Vojtěch (Head of the Department) [email protected] MALÝ Vladimír [email protected] MRVEČKA Petr [email protected] POTÁČ Michal [email protected] PSUTKA Jan (Head of the Group) [email protected] RŮŽIČKA Milan (Head of the Group) [email protected]

Technicians WITT Petr [email protected]

Administrative, Secretarial and Other Staff MÍŠKOVÁ Leona [email protected]

The Department of Military Health Service Organization is the primary department which offers military and professional training and education for students of medicine, pharmacy, military medical management, as well as for paramedical personnel to the extent necessary for the execution of their duties in a crisis and wartime health care system. It organizes postgraduate education for military doctors, pharmacists and bachelors in advanced courses. It offers specialized training in the field of military health service administration and management. It is responsible for providing career courses to military medical personnel. The subject called "Organization and Tactics of Military Medical Service" familiarizes students with the activities of the Medical Service in combat conditions, with the assignments and principals of medical support to troops, the organizational structure of the military health service in combat, the operation of particular medical establishments and the principles of human rights – in particular those defined by the Geneva Convention. The department carries out training in medical support planning, in working with map, deployment of field medical facilities, calculation of medical casualties and the Military Medical Service management. Other subjects provide students with knowledge in tactic, logistics, communications, military engineering, topography, and NBC defence. All these subjects are a part of the general military education of Czech Army

22th Annual Report, 2013 – 55 DEPARTMENT OF MILITARY HEALTH SERVICE ORGANIZATION professionals and are prerequisites of mastering the subject of military medicine. The department teaches disaster medicine, mainly focused on planning, administration, management and evacuation. The department serves as a coordinating centre for the field of research work in the discipline of the Military Medical Service Organization and Management. It participates in achieving a better quality of organization in medical units, formations, facilities, operational regulations, and management methods in foreign opeations. It sets out the basic materials and proposals from the above – mentioned areas for the Czech Army Medical Service authorities.

22th Annual Report, 2013 – 56 DEPARTMENT OF MILITARY HYGIENE DEPARTMENT OF MILITARY HYGIENE

Educational and Research Staff BEDNARČÍK Peter (Head of the Group) [email protected] FAJFROVÁ Jana (Head of the Group) [email protected] HLÚBIK Pavol (Head of the Department) [email protected] HUSÁROVÁ Michaela [email protected] PAVLÍK Vladimír [email protected] STŘÍTECKÁ Hana [email protected]

Technicians PEREGRINOVÁ Jitka [email protected]

Administrative, Secretarial and Other Staff PÍRKOVÁ Jitka [email protected]

Postgraduate Students ČMEDLOVÁ Pavlína (maternity leave) [email protected] DOHNALOVÁ Lucie

DUŠKOVÁ Klára (maternity leave, since 01 October [email protected] 2013) FAJFROVÁ Jana (till 18 September 2013) [email protected] HUSÁROVÁ Michaela [email protected] HYŠPLER Pavel

LASTIČ Miroslav

MALIŠOVÁ Zuzana

OSLADIL Tomáš

SALAJKOVÁ Šárka (since 01 October 2013)

ŠMÍDOVÁ Petra (till 01 August 2013) [email protected] VAŠINA Libor

ZMRZLÁ Hana

The Department of Military Hygiene is divided into two relatively independent groups, the work of which is interconnected and it complexly covers the basic issues of the relation of life and job environment to the health of the individual.

22th Annual Report, 2013 – 57 DEPARTMENT OF MILITARY HYGIENE

a) In the area of Hygiene of Nutrition and Occupational Hygiene, attention is paid namely to the incidence and prevalence of risk factors of non-infectious diseases of mass incidence, rational food, catering of troops in peacetime and wartime conditions – emergency food rations, assessment of the proper received and consumed energy. Occupational Hygiene is focused above all on response of the organism to work in protective clothing and severe climatic conditions and on evaluation of the degree of risk connected with environment contamination. b) The Group of Communal Hygiene is oriented on the analysis of selected physical and chemical factors of external environment in the conditions of the Army of the Czech Republic (ACR) It studies the possibilities of use of chemical substances for disinfection effects. Pedagogical activities of the Department of Military Hygiene were and still are considerably wide. The Department fully participates in a basic pre- graduate and post-graduate education of military doctors and in co-operation with the Institute for Postgraduate Medical Education in Prague; it takes part in the preparation of civilian physicians. The pre-graduate education pays attention to the students of the 3rd year of Pharmacy, the 4th and 5th year of Stomatology and especially to the students of the 5th and 6th year of General Medicine. The Department of Military Hygiene members participate in teaching basic warrants officers´ courses, commanders in reserve courses, recruited nurse’s courses.

22th Annual Report, 2013 – 58 DEPARTMENT OF PUBLIC HEALTH DEPARTMENT OF PUBLIC HEALTH

Educational and Research Staff ANTOŠ Karel [email protected] FUSEK Josef [email protected] HALAJČUK Tomáš [email protected] HLAVÁČKOVÁ Hana [email protected] HRSTKA Zdeněk (Head of the Group) [email protected] JANOUCH Jindřich [email protected] JAREŠOVÁ Lucie [email protected] JEŽEK Bruno [email protected] KULICH Marek [email protected] LAŠÁK Petr (Head of the Group) [email protected] PROCHÁZKA Miroslav (Head of the Department) [email protected] SOUKUP Ondřej [email protected] VANĚK Jan [email protected] ŽĎÁROVÁ KARASOVÁ Jana (Head of the Group) [email protected]

Educational and Research Staff / Part time PETRÁNEK Karel

Administrative, Secretarial and Other Staff HEJČOVÁ Jaroslava [email protected]

The primary focus of the Department of Public Health (K-309) is aimed at the integration of health care, pharmaceutical and managerial branches to efficiently support the Medical Service of the Czech Army, particularly in the field of disaster medicine and emergency planning, and education of medical and non-medical healthcare personnel in lifelong learning programs. The department consists of three groups – the Healthcare Management Group, the Military Pharmacy Group and the Fitness Preparation Group. Teaching activities are one of department’s main tasks. The department takes part in undergraduate study programs in military medicine, military pharmacy and military emergency response. In addition, teachers participate in the courses and training programs in health care management, medical emergency planning, crisis stress management, bio-informatics and statistics, information and computer technology, health economics, medical

22th Annual Report, 2013 – 59 DEPARTMENT OF PUBLIC HEALTH law and military career courses (basic officer course, senior officer course). The department also participates in preparation of medical troops for foreign missions. Research activities play an important role. The research is oriented towards process modelling, particularly in the context of healthcare facilities, emergency situations and major incidents, geographical information systems in emergency planning and pre hospital medical care, and medical visualization. Members of the department concentrate on the development and application of information technologies in all the mentioned fields. By actively participating in both local and international conferences, the department has established valuable co-operations with local and foreign universities. The department has been involved in international projects creating centres and methodologies for healthcare management education and for regional healthcare policies. Department’s research projects have been supported by the Czech Science Foundation, Foundation of Ministry of Health and Foundation of Ministry of Defence.

RESEARCH PROJECTS

Neurobehavioral evaluation of potential Alzhemier`s disease drugs Kassa, J., Misík, J., Kuča, K., Musílek, K., Žďárová Karasová, J. Supported by the Czech Republic Grant Agency, 2012–2015 (Project No.: GAP303/12/0611) The permanent increasing incidence of Alzheimer`s disease represents a worldwide problem that can be partly solved by introducing more effective drugs. The aim of the stuidy is to characterize the effects of newly developed acetylcholinesterase inhibitors with better pharmacodynamic features derived from the model drug 7-methoxytacrine on nervous functions and especially cognitive functions. The effects of drugs will be evaluated in laboratory rats with the deficiency of cognitive functions induced by administration of 3- chinuclidinylbenzilate. The effects of newly developed drugs will be evaluated by functional observational battery and the potency to eliminate or reduce the deficiency of cognitive functions by special neurobehavioral methods oriented on memory and learning. The effects of new drugs on nervous functions and their potency to eliminate the deficiency of cognitive functions will be compared to the effects of a model drug and standard therapeutics (tacrine, donepezil, rivastigmine). Proposed study can contribute to the increase of the effectiveness of the treatment of Alzheimer`s disease.

22th Annual Report, 2013 – 60 DEPARTMENT OF PUBLIC HEALTH

Prehospital emergency care efficiency Procházka, M., Halajčuk, T., Hrstka, Z., Ježek, B., Vaněk, J., Mašek, J. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2013–2015 (Project No.: NT14460) The main research subject of the project is assessment of suitable criteria of Emergency Medical Services (EMS) efficiency evaluation. As a part of the project an analysis of the EMS of Kralovehradecky region will be performed aimed at the economic efficiency as seen by individual EMS stakeholders (health insurance companies, EMS, regional government, Ministry of Health Care), at the EMS medical effects and at the readiness of the EMS in conjunction with the plan of the region coverage by EMS bases. To assess the aptness of the economic efficiency criteria standard economic indicators used in medical facilities will be employed to determine the criteria suitable for EMS providers. The medical effects criteria will be established in a retrospective analysis of the EMS response data. The readiness of the EMS in the region of interest will be considered by criteria extracted from the data on EMS responses including GPS data processed in GIS.

Preparation and biological evaluation of new therapeutics against to pesticides Kuča, K., Žďárová Karasová, J., Pohanka, M., Jun, D. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2011–2014 (Project No.: NT12062) The main aim of this project is to search for new and convenient therapeutics that will be more effective in therapy of pesticide poisoning (pesticides are commonly used organophosphate inhibitors of cholinesterases). New structures will be determined by using highly sophistic methodology: molecular design. Predicted structures and their analogues will be synthesized. Their therapeutic potency will be tested in in vitro studies. The acetylcholinesterase reactivators with the highest reactivation potency will be chosen into in vivo tests. During this development process other important data such as LD50 of newly synthesized therapeutics, biochemical studies and pharmacokinetics studies (distribution of newly prepared therapeutics and their ability to penetrate through the blood-brain barrier into central nervous system) will be also replenished. If some effective candidates will be found, subsequent cooperation with foreign institutes is presumed.

22th Annual Report, 2013 – 61 DEPARTMENT OF RADIOBIOLOGY DEPARTMENT OF RADIOBIOLOGY

Educational and Research Staff ŠINKOROVÁ Zuzana [email protected] TICHÝ Aleš [email protected] VÁVROVÁ Jiřina (Head of the Department) [email protected] ZÁRYBNICKÁ Lenka [email protected]

Technicians MERVARTOVÁ Lenka [email protected] PRŮCHOVÁ Šárka [email protected]

Postgraduate Students ĎURIŠOVÁ Kamila

HLÁVKA Aleš

KAŠAOVÁ Linda [email protected] KMOCHOVÁ Adéla (since 01 October 2013)

LALÁKOVÁ Lucie (till 12 August 2013)

NOVOTNÁ Eva [email protected]

The Department of Radiobiology was established at Purkyně Military Medical Research and Postgraduate Institute on September the 1st 1963. The first chief of the department became Colonel Prof. MUDr. Josef Mráz, CSc., who was in 1968 appointed the first professor of military radiobiology of the Charles University. The main tasks of the department are teaching and research activities, which are closely connected. In the field of research, the experimental work includes histology and cytology, in vitro methods, methods of proteomic analysis and methods of flow cytometry. Individual technological units allow in vitro and in vivo observation of post-radiation mechanisms on molecular, cellular, and organ levels. Military research is focused on early diagnosis and therapy of post- radiation damage in the main objective of the department. The aim of investigation in the medium-term horizon is discovery and practical introduction of bio-dosimeter markers as well as continuous renewal of decontamination agents for the Army of CR. Mutual cooperation with other NBC research workplaces also remains an integral part of our research activities. Cooperation with civilian workplaces at the Faculty of Medicine and the University Hospital in Hradec Králové is focused on radiation oncology.

22th Annual Report, 2013 – 62 DEPARTMENT OF RADIOBIOLOGY

The Department of Radiobiology takes part in military medical-specialist education in the form of pre-gradual and post-gradual education mainly in doctoral studies. The main educational activity is lecturing military radiobiology. The main topics are: the nuclear weapons effects on the living organism, the possibilities of the protection and medical treatment of irradiated persons. Other special military issues are disaster medicine, NBC protection etc., which are taught at the Faculty of Military Health Sciences, including the topics, which are presented by the instructors of our department.

RESEARCH PROJECTS

Phosphoproteomic analysis of leukaemic cells after irradiation Tichý, A. Supported by the Czech Republic Grant Agency, 2012–2014 (Project No.: GPP206/12/P338) Phosphorylation is one of the most important post-translational modifications, which affects protein structure, function, and localization, regulating majority of biochemical processes. In our previous work we were engaged with molecular mechanisms of response of leukaemic cells MOLT-4 (p53-positive) and HL-60 (p53-negative)to ionising radiation. Submitted project proposes to characterize phosphoproteome of the mentioned cells, i.e. to identify phosphoproteins which are involved in radiation-induced signaling using tandem mass spectrometry, and to propose the potential biodosimetric markers. To date, no study has been published in the presented area mainly due to a very low abundance of phosphoproteins in the cell and due to insufficient ionization during MS analysis (acidic and hydrophilic character) compared to non-modified proteins. Therefore we will employ the newest methods for selective enrichment of phosphopeptides (bioaffinity chromatography) and recent knowledge to increase enrichment efficiency (methylation, pH adjustment etc.).

Radio-sensibilization of MOLT-4 and SAOS-2 cell lines by DNA repair inhibition: Phosphoproteomic analysis of irradiated cancer cells Šalovská, B., Tichý, A. Supported by Grant Agency of Charles University, 2013–2013 (Project No.: GAUK 2013) The project is based on the study of phosphorylation – one of the most considerable post-translational modifications. To induce phosphorylation we employ ionizing radiation, which is used in radiotherapy of cancer since its capable of induction of DNA damage and apoptosis. In order to increase radiosensitivity, it is often combined with various radiosensitizers

22th Annual Report, 2013 – 63 DEPARTMENT OF RADIOBIOLOGY

Hence, the main goal is to compare the effect of three radiosensitizers – KU55339, NU7441, and VE-821 that specifically inhibit key kinases regulating DNA repair after irradiation – ataxia-telangiectasia mutated kinase, DNA-dependent protein kinase a ATM-Rad3-related kinase. Each of these kinases phosphorylates a number of other substrates, some of them specifically and some redundantly and triggers a specific signalling cascade. Consequently, inhibition of DNA repair enzymes increases apoptosis and allows eradication of a cancer cells. Since we use a specific inhibitor for each particular kinase, we aim to exploit mass spectrometry for identification and quantification of proteins involved in the mentioned pathways and to investigate their role in the p53-negative cellular environment.

22th Annual Report, 2013 – 64 DEPARTMENT OF TOXICOLOGY DEPARTMENT OF TOXICOLOGY

Educational and Research Staff BAJGAR Jiří [email protected] CABAL Jiří (Head of the Group) [email protected] KASSA Jiří (Head of the Department) [email protected] KORÁBEČNÝ Jan [email protected] KRAČMAROVÁ Alžběta [email protected] MISÍK Jan [email protected] MUSÍLEK Kamil [email protected] SVOBODOVÁ Hana [email protected] ŠEPSOVÁ Vendula [email protected] ŠTĚTINA Rudolf (Head of the Group) [email protected]

Technicians RESLOVÁ Eva

ŠKRANCOVÁ Věra

UHLÍŘOVÁ Jana

Postgraduate Students ANDRŠ Martin (since 01 October 2013)

BENEK Ondřej [email protected] COUFALOVÁ Klára (till 31 May 2013) [email protected] JÍLKOVÁ Martina [email protected] JOŠT Petr [email protected] KAŠPÁREK Aleš (till 01 October 2013) [email protected] MISÍK Jan (till 24 January 2013) [email protected] NEPOVIMOVÁ Eugenie (since 01 October 2013)

PAVLÍK Michal [email protected] PAVLÍKOVÁ Růžena [email protected] PAVLIŠ Oto [email protected] PEJCHAL Jaroslav [email protected] ŠEPSOVÁ Vendula [email protected] ŠPILOVSKÁ Katarína [email protected]

22th Annual Report, 2013 – 65 DEPARTMENT OF TOXICOLOGY

VOBORNÍKOVÁ Irena [email protected] ZEMEK Filip

The Department was established in 1951. Since then, as an integral part of the Faculty of Military Health Sciences, it has been involved in education and scientific research work on chemical warfare agents for defensive and protective purposes only. It comprises two laboratory groups – a biochemical (biochemical laboratory, laboratory of organic synthesis, analytical laboratory, decontamination laboratory) and an experimental therapy group (toxicological laboratory, pharmacological laboratory, neurophysiological laboratory, neurobehavioral laboratory, genotoxicological laboratory). This structure permits the complex study of highly toxic substances including chemical warfare agents with aims to determine their action on biochemical, neurobehavioral, histochemical, pharmacological and neurophysiological level, to study and develop antidotes, to analyse all types of samples with respect to the presence of known chemical warfare agents, to test decontamination effectiveness of developed and field decontamination kits. Present scientific research projects are focused on therapeutic, prophylactic and protective measures against the most toxic chemical warfare agents. Special attention has been paid to the most recent and most dangerous nerve agents and mustards. The main educational activity task was to give lectures for undergraduate and post-graduate studies dealing with problems of biological effects of real and potencial chemical warfare agents, the possibilities of the medical and chemical protection against them and the approaches to medical care of persons intoxicated with chemical warfare agents, especially nerve agents. The Department of Toxicology also participates in the teaching of toxicology in disaster medicine.

RESEARCH PROJECTS

Neurobehavioral evaluation of potential Alzhemier`s disease drugs Kassa, J., Misík, J., Kuča, K., Musílek, K., Žďárová Karasová, J. Supported by the Czech Republic Grant Agency, 2012–2015 (Project No.: GAP303/12/0611) The permanent increasing incidence of Alzheimer`s disease represents a worldwide problem that can be partly solved by introducing more effective drugs. The aim of the stuidy is to characterize the effects of newly developed acetylcholinesterase inhibitors with better pharmacodynamic features derived from the model drug 7-methoxytacrine on nervous functions and especially cognitive functions. The effects of drugs will be evaluated in laboratory rats with the deficiency of cognitive functions induced by administration of 3- chinuclidinylbenzilate. The effects of newly developed drugs will be evaluated by functional observational battery and the potency to eliminate

22th Annual Report, 2013 – 66 DEPARTMENT OF TOXICOLOGY or reduce the deficiency of cognitive functions by special neurobehavioral methods oriented on memory and learning. The effects of new drugs on nervous functions and their potency to eliminate the deficiency of cognitive functions will be compared to the effects of a model drug and standard therapeutics (tacrine, donepezil, rivastigmine). Proposed study can contribute to the increase of the effectiveness of the treatment of Alzheimer`s disease.

COOPERATION In 2013, the Department of Toxicology has continued in the cooperation with various research institutes (the National Poison Control Centre, Military Medical Academy, Belgrade – Republic of Serbia, Medicinal Science Division, Korea Research Institute of Chemical Technology, Taejon – Korea, Institute for Medical Research and Occupational Health, Zagreb – Croatia, Bundeswehr Institute of Pharmacology and Toxicology, Munich – Germany, Florida International University, Herbert Wertheim College of Medicine in USA, University of St. Andrew, School of Biology in United Kingdom, UAE University, Faculty of Medical Research Sciences in United Arabian Emirates, Semmelweis University, Faculty of Pharmacy in Hungary, Institute de Recherches Biomédical des Armeés in France) in the field of development of prophylactic and therapeutical means against nerve agents and organophosphorous insecticides. The cooperation has been mostly characterized by the exchange of scientific information. Within the frame of the work dealing with the identification of the mechanisms of chemoprevention in the initial phases of mutageneses and carcinogenesis, the Department of Toxicology has also continued in cooperation with the Institute Nutrition Research in Oslo (Norway) and the Institute of Experimental Oncology and the Slovak Health Care University in Bratislava (Slovak Republic). The cooperation with Department of Organic Chemistry, Institute of Chemistry, P. J. Šafárik University in Košice (Slovak Republik), Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences in Košice (Slovak Republic) and Faculty of Chemical and Food Technology, Slovak Technical University in Bratislava (Slovak Republic) is continuing in the field of the development of new potential therapeutic means against Alzheimer disease.

22th Annual Report, 2013 – 67 INSTITUTE OF MOLECULAR PATHOLOGY INSTITUTE OF MOLECULAR PATHOLOGY

Educational and Research Staff BALONOVÁ Lucie [email protected] KLIMENTOVÁ Jana [email protected] KROČOVÁ Zuzana (Head of the Group) [email protected] LENČO Juraj [email protected] LINK Marek [email protected] MACELA Aleš (Prof. Emerit.) [email protected] PÁVKOVÁ Ivona [email protected] ŘEHULKA Pavel (Head of the Group) [email protected] STULÍK Jiří (Head of the Institute) [email protected] ŠPIDLOVÁ Petra [email protected]

Technicians LUKŠÍKOVÁ Lenka (maternity leave) [email protected] ŠAFÁŘOVÁ Mária [email protected]

Administrative, Secretarial and Other Staff FORETOVÁ Zdenka [email protected]

Postgraduate Students DAŇKOVÁ Věra

DRESLER Jiří (till 13 September 2013)

FABRIK Ivo [email protected] FUČÍKOVÁ Alena (till 27 September 2013) [email protected] HOSTÝNKOVÁ Veronika [email protected] NEUBAUEROVÁ Věra [email protected] PLZÁKOVÁ Lenka (since 01 October 2013)

PUTZOVÁ Daniela

SCHMIDT Monika [email protected] ŠENITKOVÁ Iva senitkovpmfhk.cza VAJRYCHOVÁ Marie

22th Annual Report, 2013 – 68 INSTITUTE OF MOLECULAR PATHOLOGY

The Institute of Molecular Pathology, as one of the successors of the Institute of Radiobiology and Molecular Pathology, is a research centre focused on the application of advanced technologies of functional genomics to bio-medical defence research. Scientific work is preferably aimed at the studies of the host-pathogen interactions at the molecular level. The objectives of this research splits in three main topics: biomolecular signatures of biological agents potentially abused for the military, terroristic or bio-crime acts, intracellular fate of ingested microbes and finally the modulation of host cell signalling and gene expression by ongoing infection. The favourite microbial model is live vaccine strain of Francisella tularensis, a gram-negative facultative intracellular bacterial pathogen from the gamma subdivision of Proteobacteriae. The second branch of the research involves the clinical studies utilizing the post-genomic approaches for identification of new biomarkers of different pathological processes. Laboratories of the Institute are currently equipped for realization of complete classical and shotgun proteomic analyses. The materials for analyses are prepared in the Institute’s tissue culture and microbiological labs. In parallel, the basic search for gene expression can be performed using quantitative real-time PCR technology. The established technologies enable researchers, Ph.D. students, and under-graduate students to realize complex studies oriented on the analyses of living system response to external (and internal, modulatory) signals encompassing the chemicals, biologically active bio-molecules, physical influences (temperature, radiation, etc.), and microorganisms. During the year 2008 the Institute traditionally cooperated with the military medical and research facility in Sweden (FOI NBC-Defence, Umea) on preparation of Francisella tularensis knock-outs. The further scientific contacts involved National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, USA – mass spectrometry analysis of bacterial glycoproteins, Unité de Pathogénie des Infections Systémiques, Faculté de Médecine, Necker-Enfants Malades, Paris, France – comparative proteomic studies of Francisella tularensis deletion mutants, U.S. Army Medical Research Institute of Infectious Disease (Fort Detrick, USA) – preparation and testing of bacterial proteins with immunostimulatory potential and, finally Department of Microbiology and Parasitology, University of Rijeka – microscopical analysis of microbial intracellular trafficking, Molecular Infection Medicine, University of Umea, Sweden – analysis of host-pathogen interactions. Within the frame of the Czech Republic, the Institute has useful contacts with the Institute of Microbiology, Czech Academy of Science, Prague, the Faculty of Science, Charles University, Prague, the University Hospital in Hradec Králové, Department of Pharmacology of Medical Faculty in Hradec Králové, Department of Oncological and Experimental Pathology Masaryk Memorial Cancer Institute, Brno and Veterinary Research Institute in Brno.

22th Annual Report, 2013 – 69 INSTITUTE OF MOLECULAR PATHOLOGY

The financial support for research activities performed in the collaboration with above-mentioned Institutes comes from the programmes and projects of Czech Grant Agencies and Ministry of Defence. Currently, the Institute for Molecular Pathology has 11 full-time permanent employees, 9 scientists, 1 technician and 1 administrative worker. The Institute has currently 10 PhD students and, furthermore, several undergraduates have been working on their diploma thesis in the Institute.

RESEARCH PROJECTS

Characterization of the diagnostic potential of native polypeptides in amniotic fluid Lenčo, J. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2012–2015 (Project No.: NT13599) Preterm premature rupture of membranes (PPROM) is a principal cause of preterm births and considerably increases perinatal morbidity and mortality. The main reason is the causal link with intraamniotic infection (IAI), which occurs in 40–60 % of PPROM patients. The most serious cases of IAI may result in fetal inflammatory response (FIRS) and cause permanent health consequences for the newborn or end up by the death of the fetus. FIRS is very often subclinical and thus frequently remains undetected. Currently, there is no tool for its precise prenatal diagnostics available. The project is focused on characterization of the diagnostic potential of native amniotic fluid polypeptides with regard to detection of IAI and FIRS.

Identification of novel Francisella tularensis targets for subunit vaccine development Stulík, J., Worsham, P. Supported by the Defense Threat Reduction Agency, 2011–2014 (Project No.: D-CZ-10-0001) To develop a subunit vaccine for tularemia. Specifically, this project focuses on (1) the identification of surface associated or secreted virulence factors from F. tularensis using immunoproteomic approaches (2) cloning and expression of these gene products, (3) confirming the role of the selected targets in virulence, and (4) assessing these proteins as protective antigens in animal models.

22th Annual Report, 2013 – 70 INSTITUTE OF MOLECULAR PATHOLOGY

New technologies for identification and typing of biological agents Kročová, Z., Boštík, P., Hanovcová, I., Jun, D., Macela, A. Supported by the Czech Republic Ministry of Internal Affairs, 2012–2015 (Project No.: VF20122015024) The aim of the project is to develop the methodological procedures for the isolation of bacterial and viral nucleic acids and protein and no-protein toxins from natural matrices, and the procedures for their identification and typing. In the case of bacteria and viruses are designed following the methodological procedures and specific technological and laboratory units: the acquisition and cultivation of biological agents, isolation of genome and plasmid DNA, or RNA in the case of a virus, the methodology and procedures for the preparation of samples of bacterial and viral nucleic acids from complex matrices, design of qPCR primers, probes and the reaction conditions and testing and validation of the proposed methods and procedures for the identification of biological agents. For the detection of low molecular weight toxins will be used high performance liquid chromatography coupled with tandem mass spectrometry and for detection and identification of protein toxins will be used mass spectrometric method SRM (Selected reaction monitoring).

Proteome analysis of the interaction of host cell with intracellular pathogen Stulík, J. Supported by the Ministry of Education, Youth and Sports, 2012–2013 (Project No.: 7AMB12GR038) Project Mobility Czech Republic – Greece. The major aim of the proposed project is the common exploitation of advanced proteome high-throughput technologies, established in different scope in both laboratories, for identification of key host cell protein molecules whose quantity ad/or posttranslational modification is modulated by the early phase of host-pathogen interaction. Furthermore using bioinformatic tools the probable functions and the role of these identified proteins in the host protective response against infection will be predicted.

Targeted proteomic analysis in hypertrophic cardiomyopathy Stulík, J., Fučíková, A. Supported by the Internal Grant Agency of the Czech Republic Health Service, 2012–2015 (Project No.: NT13721) The basic objective of the proposed project is to verify the analytical potential of selected biomarkers of peripheral blood for diagnostics of hypertrophic cardiomyopathy. In our previous study, we used quantitative proteomics to identify 40 proteins in plasma. The plasma concentrations of these proteins were significantly different compare to control group.

22th Annual Report, 2013 – 71 INSTITUTE OF MOLECULAR PATHOLOGY

The scope of the proposed project is the verification and validation of the concentration of these proteins in peripheral blood of patients with hypertrophic cardiomyopathy; the comparison of the results with the healthy population and other diseases that are accompanied by structural changes in the myocardium (dilated cardiomyopathy, ischemic heart disease, arterial hypertension and aortic stenosis). The project will use commercially available kits based on the detection and quantification using antibodies and antibody-independent method for proteomic SRM (Selected Reaction Monitoring).

The role of B cells during natural and adaptive phase of immune response against Francisella tularensis infection in mice Kročová, Z. Supported by the Czech Republic Grant Agency, 2011–2014 (Project No.: GAP302/11/1631) Tularemia is a zoonotic infection with high infectivity and high morbidity. The etiological agent of tularemia is Francisella tularensis, an intracellular pathogen on all major lists of potential bioterroristic agents. The live vaccine strain without the license for human use is still the only problematic tool for the immunoprophylaxis. In spite of a growing number of studies dedicated to immunity against tularemia, the mechanisms participating in the protective response remain mostly unknown. Especially the role of B cells and specific antibodies is a recently hot topic of debate. For this reason the proposal of this project covers the aims concerning the mechanisms by which B cells and antibodies participate in protective response, e.g. antigen presenting function, production of specific antibodies during the T-independent phase of innate response, B cell mediated immunological memory and the existence of an analogy of ADCC where bacteria are the targets. No direct experimental evidence exists for the participation of these phenomena in protective immunity against tularemia.

The role of virus associated cellular proteins in T-lymphocyte dysfunction Boštík, P., Řehulka, P., Pejchal, J., Boštíková, V., Kročová, Z. Supported by the Czech Republic Grant Agency, 2010–2014 (Project No.: GAP304/10/1161) Herpetic viruses, such as VZV, and lentiviruses, such as HIV or SIV, are enveloped viruses, which infect CD4 T cells and cause transient (VZV) or progressive (SIV) dysregulation of T cell function. This effect is mainly indirect, as the fraction of infected cells is small, but the dysregulatory effect is observed in much larger cell population. These viruses incorporate host- derived proteins into their envelopes during the process of virus maturation and these proteins can either retain their function or engage their receptors and subsequently initiate intracellular signaling. This can be mediated by Akt-

22th Annual Report, 2013 – 72 INSTITUTE OF MOLECULAR PATHOLOGY

GSK3 pathway and PGE metabolism, leading to T cell dysfunction and apoptosis. This proposal utilizes state-of the art proteomic approach to Identification of host cell proteins incorporated into the SIV and VZV virions. The role of these host cell proteins will be subsequently investigated in their effects on CD4 T cell signaling cascades and can therefore lead to the elucidation of mechanisms involved in CD4 T cell dysfunction and death in such diseases as chickenpox and AIDS.

22th Annual Report, 2013 – 73 VIVARIUM VIVARIUM

Educational and Research Staff PAVLÍK Michal (Head of the Department) [email protected]

Technicians BOCKOVÁ Irenea (Head of the Group) ETFLAIŠOVÁ Petra JUNKOVÁ Irena PUDÍKOVÁ Margita SLAVÍK Jaroslav

The Vivarium at the Faculty of Military Health Sciences of the University of Defence fulfils science, research and teaching tasks of the departments and specialized workplaces of the Faculty of Military Health Sciences. The vivarium is subordinated to the Department of Teaching support. The separate vivarium for mice and sewer-rats is a part of the Department of Toxicology. From the point of view of the capacity the Faculty of Military Health Sciences is able to carry out experiments, place and take care of laboratory mice, sewer-rats, guinea-pigs, rabbits, pigs or mini pigs. In the area of the vivarium there are also laboratory workplaces and operating theatres, which are equipped for experiments on laboratory animals. All studies have to be allowed by the Ethical Board of the Faculty of the Military Health Sciences fully in compliance with the legal standards of the protection against cruelty to animals. The vivarium with the operating block is intesively used above all for experiments on large experimental animals. The courses BATLS and BARTS are held there. During the courses different model situations and cases of emergency medicine are performed for military doctors and participants of foreign mission including war injuries on dead and live experimental animals. On 30 December 2010 the vivarium was accredited for use again for 5 years (till 30 December 2015) by the Central Board for Animal Protection . The above-mentioned range of the activities shows that it is necessary to time work and also co-ordinate it personally including permanent presence of a veterinary surgeon, veterinary technicians and breeders of laboratory animals.

22th Annual Report, 2013 – 74 VISITS ABROAD VISITORS TO THE FACULTY OF MILITARY HEALTH SCIENCES

Austria Ernst Pittenauer (Vienna) – Scientific collaboration, 02 December 2013–06 December 2013 Greece Katerina Arvaniti (Iraklion) – Training in lipid raft isolation and microgradient separation of peptides, 14 July 2013–20 July 2013 Hungary Anita Jekö (Budapest) – Erasmus, 01 November 2013–01 February 2014

VISITS ABROAD

Austria Chlíbek, R. (15th ISW-TBE Meeting will Take Place, Vienna, 31 January 2013–01 February 2013) Klein, L. (15th European Burns Association Congress, Vienna, 27 August 2013–31 August 2013) Vajrychová, M. (Late summer practical proteomic seminar, Vienna, 02 September 2013–03 September 2013) Žďárová Karasová, J. (21th WCN 2013, Vienna, 21 September 2013–26 September 2013) Belgium Boštík, P. (CapTech ESM04 EDA meeting, Brussels, 07 November 2013–08 November 2013) Chlíbek, R. (Combination Vaccines Academy, Brussels, 09 May 2013–10 May 2013) Kassa, J. (34th NATO CBRN Medical Working Group Meeting, Brussels, 10 February 2013–15 February 2013) Brazil Boštík, P. (1st CBRN Threat Seminar, Rio de Janeiro, 26 November 2013–01 December 2013)

22th Annual Report, 2013 – 75 VISITS ABROAD

Bulgaria Halajčuk, T. (12th International Conference Economy & Business, Sunny Beach, 01 September 2013–05 September 2013) Procházka, M. (12th International Conference Economy & Business, Sunny Beach, 01 September 2013–05 September 2013) France Zárybnická, L. (InnovaCom: Education for Competitiveness, Paris, 20 November 2013–20 November 2013) Zárybnická, L. (Internhip of the Operational Programme ”Education for Competitiveness“, Paris, 29 October 2013–28 November 2013) Germany Boštík, P. (CapTech ESM04 EDA meeting, Ottobrunn, 18 June 2013–20 June 2013) Dohnalová, L. (International Summer Lab (ISL) – Course "Food Process Design", University of Applied Sciences, Quakenbrueck, 07 July 2013–19 July 2013) Chlíbek, R. (2nd Conference on Controversies in Vaccination in Adults, Munich, 14 February 2013–17 February 2013) Kassa, J. (14th International Medical Chemical Defence Conference, Munich, 23 April 2013–25 April 2013) Musílek, K. (14th International Medical Chemical Defence Conference, Munich, 23 April 2013–25 December 2013) Greece Stulík, J. (Working Meeting at Department of Chemistry, University of Crete, Iraklion, 13 November 2013–16 November 2013) Hungary Kubelková, K. (EFIS–EJI Sympozium IMPULSE, Matrahaza, 31 August 2013–04 September 2013) China Šepsová, V. (14th International Symposium on Cholinergic Mechanisms, Hangzhou, 05 May 2013–09 May 2013)

22th Annual Report, 2013 – 76 VISITS ABROAD

Israel Boštík, P. (10th International Consequence Management Seminar, Tel Aviv, 06 October 2013–10 October 2013) Páral, J. (4th World Congress for the Advancement of Surgery, Tel Aviv, 27 October 2013–31 October 2013) Italy Chlíbek, R. (31st Meeting of the European Society for Paediatric Infectious Diseases, Milan, 28 May 2013–31 May 2013) Kassa, J. (AD/PD™ 2013: 11th International Conference on Alzheimer’s and Parkinson’s Diseases, Florence, 06 March 2013–10 March 2013) Kročová, Z. (15th International Congress of Immunology, Milan, 22 August 2013–27 September 2013) Kubelková, K. (15th International Congress of Immunology, Milan, 22 August 2013–28 August 2013) Lochman, P. (10th International Gastric Cancer Congress, Verona, 18 June 2013–22 June 2013) Misík, J. (AD/PD™ 2013: 11th International Conference on Alzheimer’s and Parkinson’s Diseases, Florence, 06 March 2013–10 March 2013) Musílek, K. (AD/PD™ 2013: 11th International Conference on Alzheimer’s and Parkinson’s Diseases, Florence, 06 March 2013–10 March 2013) Smetana, J. (31st Meeting of the European Society for Paediatric Infectious Diseases, Milan, 28 May 2013–31 May 2013) Šepsová, V. (AD/PD™ 2013: 11th International Conference on Alzheimer’s and Parkinson’s Diseases, Florence, 06 March 2013–10 March 2013) Žďárová Karasová, J. (AD/PD™ 2013: 11th International Conference on Alzheimer’s and Parkinson’s Diseases, Florence, 06 March 2013–10 March 2013) Japan Vajrychová, M. (12th World congress HUPO, Yokohama, 12 September 2013–19 September 2013) Norway Klein, L. (Worshop and Seminar on Health Disaster Management, Oslo, 09 June 2013–14 June 2013)

22th Annual Report, 2013 – 77 VISITS ABROAD

Poland Hlúbik, P. (8th National Scientific Conference, Biala Podlaska, 03 September 2013–06 September 2013) Chlíbek, R. (6th Pneumo Surveillence Workschop, Kraków, 16 April 2013–18 May 2013) Kročová, Z. (ESF-EMBO Symposium with support from EFIS: B Cells from Bedside to Bench and Back Again, Pultusk, 02 September 2013–07 September 2013) Republic of Korea Kuča, K. (Korean Research Institute of Chemical Technology, Daejon, 15 October 2013–15 October 2013) Serbia Chlíbek, R. (3rd CEEPAG Meeting Agenda, Belgrade, 29 January 2013–31 January 2013) Slovakia Chlíbek, R. (14th Slovak Immunisation Congress, Štrbské Pleso, 17 January 2013–19 January 2013) Chlíbek, R. (Adolescent Vaccination, Bratislava, 25 April 2013– 27 April 2013) Chlíbek, R. (17th Slovak-Czech Congress on Infections Diseases, Poprad, 13 June 2013–15 June 2013) Chlíbek, R. (10th Slovak Paediatric Congress, Bratislava, 25 April 2013–27 April 2013) Kubelková, K. (Virological Institute in Bratislava, Bratislava, 11 March 2013–22 March 2013) Pavlík, V. (Living Conditions and Health, Nový Smokovec, 23 September 2013–25 September 2013) Svobodová, H. (Genetic toxicology and prevention of cancer, Smolenice, 13 October 2013–16 October 2013) Svobodová, H. (FEBS workshop – Nucleotide excision repair and interstrand crosslink repair, Smolenice, 09 June 2013–13 June 2013) Šinkorová, Z. (Disaster Crisis Management, Žilina, 05 June 2013–06 June 2013) Štětina, R. (FEBS workshop – Nucleotide excision repair and interstrand crosslink repair, Smolenice, 09 June 2013–13 June 2013) Šubrt, Z. (67th Surgery Day of Kostlivy, Bratislava, 06 December 2013–06 December 2013)

22th Annual Report, 2013 – 78 VISITS ABROAD

South Africa Chlíbek, R. (8th World Congress of the World Society for Pediatric Infectious Diseases WSPID, Cape-town, 18 November 2013–24 November 2013) Smetana, J. (8th World Congress of the World Society for Pediatric Infectious Diseases WSPID, Cape-Town, 18 November 2013–24 November 2013) Spain Boštíková, V. (CDC Advisory Board, Malaga, 07 October 2013– 11 October 2013) Kassa, J. (26th ECNP Congress, Barcelona, 05 October 2013– 10 October 2013) Misík, J. (26th ECNP Congress, Barcelona, 05 October 2013–10 October 2013) Pohanka, M. (26th ECNP Congress, Barcelona, 05 October 2013–10 October 2013) Střítecká, H. (20th International Congress of Nutrition, Granada, 15 September 2013–20 September 2013) Špliňo, M. (CDC Advisory Board, Malaga, 08 September 2013– 13 September 2013) Sweden Kupsa, T. (18th Congress of European Hematology Association (EHA), Stockholm, 12 June 2013–16 June 2013) Šepsová, V. (Shalgagreska Institut University Gothenburg, Gothenburg, 17 November 2013–30 November 2013) Zárybnická, L. (CELOD: Cellular Effects of Low Doses and Low Dose-Rates with Focus on DNA Damage and Stress Response, Stockholm, 21 April 2013–03 May 2013) Switzerland Šmejkal, K. (AOTrauma Masters Course – Complications and Complex Fractures, Davos, 08 December 2013–13 December 2013) Turkey Horáček, J. (4th International Congress on Leukemia Lymphoma Myeloma (ICLLM), Istanbul, 21 May 2013–26 May 2013) Chlíbek, R. (EURIT Regional Advisory Board on Adult Vaccination against Pneumococcal Diseases, Istanbul, 25 October 2013–27 October 2013) Stulík, J. (International Symposium on Francisella tularensis and Tularemia, Urgup, 18 June 2013–23 June 2013)

22th Annual Report, 2013 – 79 VISITS ABROAD

Špidlová, P. (International Symposium on Francisella tularensis and Tularemia, Urgup, 18 June 2013–23 June 2013) United Kingdom Hlúbik, P. (ECO 2013, Liverpool, 12 May 2013–16 May 2013) Jebavý, L. (39th Annual Meeting of the European Group for Blood and Marrow Transplantation (EBMT), London, 06 April 2013–10 April 2013) Soukup, O. (Postdoc Intership, St. Andrews, 28 April 2013–18 June 2013) Střítecká, H. (ECO 2013, Liverpool, 12 May 2013–16 May 2013) United States Boštíková, V. (Viral infections, Atlanta, 01 December 2013–09 December 2013) Horáček, J. (55th Annual Meeting of American Society of Hematology (ASH), New Orleans, LA, 04 December 2013–12 December 2013) Korábečný, J. (Neuroscience 2013 – annual meeting SfN, San Diego, 08 November 2013–14 December 2013) Kuča, K. (Neuroscience 2013 – annual meeting SfN, San Diego, 08 November 2013–14 November 2013) Kupsa, T. (55th Annual Meeting of American Society of Hematology (ASH), New Orleans, LA, 04 December 2013–12 December 2013) Misík, J. (Neuroscience 2013 – annual meeting SfN, San Diego, 08 November 2013–14 November 2013) Musílek, K. (Alzheimer´s Association International Conference 2013, Boston, 13 July 2013–18 July 2013) Pohanka, M. (Modulation of immunization via cholinergic nervous system using HI-6, Baltimore, 19 November 2013–24 November 2013) Soukup, O. (Neuroscience 2013 – annual meeting SfN, San Diego, 08 November 2013–14 November 2013) Šinkorová, Z. (28th Congress of the International Society for Advancement of Cytometry – Cyto 2013, San Diego, 19 May 2013–23 May 2013) Vietnam Klein, L. (9th Asia-Pacific Burns Conference (ISBI), Hanoi, 31 March 2013–07 April 2013)

22th Annual Report, 2013 – 80 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013

Military Hygiene Specialized course – Evaluation of workload, 04 November 2013–06 November 2013 Specialized course – Teachings about foodstaf, 02 April 2013– 04 April 2013 Specialized course – Hygienic support to missions, 04 March 2013–06 March 2013 Specialised course – Work environment and occupational hygiene, job classification, 27 February 2013–27 February 2013 Specialized course – Nutrition support of patients with overweight and obesity, 11 March 2013–13 March 2013, 25 November 2013–27 November 2013 Specialized course – Obesity, diagnostic possibilities, psychological aspects, therapeutical approach, 09 September 2013–11 September 2013 Specialised course – Providing personal protective equipment (PPE), washing, cleaning and disinfective agents and protective drinks in the workplace, 26 February 2013–26 February 2013 Specialized course – Evaluation of the nutritional state of the organism, 14 January 2013–16 January 2013 Language Courses Refresher language courses – English (STANAG 2), 21 October 2013–29 November 2013 Combined language courses – English (STANAG 3), 03 September 2012–31 May 2013, 09 September 2013–30 May 2014 Intensive language courses – English (STANAG 1), 09 September 2013–13 December 2013 Refresher language courses – English (STANAG 1), 09 September 2013–18 October 2013 Medical English conversation course, 11 February 2013–22 February 2013 Combined language courses – French (STANAG 2), 17 September 2012–14 June 2013 Combined language courses – English (STANAG 2), 09 September 2013–30 May 2014

22th Annual Report, 2013 – 81 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013

Military Medical Service Organization Specialized course of aeromedical evacuation, 16 April 2013– 18 April 2013 Specialized warrent officer’s course for non-medical personnel, 08 April 2013–14 June 2013 Senior staff officer course – Military health service organization and management, 16 September 2013–27 June 2014, 03 September 2012–14 June 2013 Course for officers – Military health service organization and management, 07 January 2013–03 May 2013 Special course – characteristics of ACR Military Medical Service function, 12 February 2013–14 February 2013 MEDEVAC specialized course – Use of helicopter, 07 October 2013–11 October 2013 Purpose course for members of medical service before mission abroad, 21 January 2013–22 January 2013 Military Epidemiology Specialized course – Epidemiology of new infectious diseases, 22 January 2013–24 January 2013 Specialized course – Basis in tropical and travel medicine, 05 November 2013–07 November 2013 Specialized course – Infectious diseases prevention, 12 November 2013–14 November 2013 General Medicine Specialized course – Battlefield Advanced Trauma Life Support (BATLS), 25 February 2013–27 February 2013, 02 April 2013– 04 April 2013, 04 November 2013–06 November 2013 Specialized course – Teaching methodology of health training, 08 April 2013–12 April 2013 Special course of first aid in the field, 14 January 2013–18 January 2013, 28 January 2013–01 February 2013, 04 February 2013–08 February 2013, 18 February 2013–22 March 2013, 02 September 2013–06 September 2013, 23 September 2013–27 September 2013 Special course of first aid in the field for VO 84, 04 February 2013–08 February 2013 Innovative course – Battlefield Advanced Resuscitation Techniques and Skills (R-BARTS), 04 March 2013–06 March 2013, 09 September 2013–11 September 2013 Specialized course – Battlefield Advanced Resuscitation Techniques and Skills (BARTS), 25 March 2013–27 February 2013, 02 April 2013–04 April 2013, 04 November 2013–06 November 2013

22th Annual Report, 2013 – 82 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013

Special course of health training for Military High School Moravska Trebova, 04 June 2013–06 June 2013, 11 June 2013–13 June 2013, 18 June 2013–20 June 2013 Specialized course – Defibrilators and their operation, 10 January 2013–10 January 2013, 30 April 2013–30 April 2013, 12 September 2013–12 September 2013, 03 October 2013–03 October 2013 Specialized course – Transportation of casualties in the field, 22 April 2013–26 April 2013, 17 June 2013–21 June 2013 Special course of first aid in the field for Police of the Czech Republic, 15 April 2013–19 April 2013 Special course in extended first aid in the field for the Police of the Czech Republic (CLS Course), 13 May 2013–24 May 2013 Special course in extended first aid in the field (CLS Course), 18 March 2013–29 March 2013, 03 June 2013–14 June 2013, 07 October 2013–18 October 2013, 18 November 2013–29 November 2013 Special course – Repetitory of extended first aid in field conditions (R-CLS Course), 11 November 2013–15 November 2013 Innovative course – Battlefield Advanced Trauma Life Support (R – BATLS), 04 March 2013–06 March 2013, 09 September 2013–11 September 2013 Computing Special course – MS Access, 28 January 2013–01 February 2013, 30 September 2013–04 October 2013 Special course – MS PowerPoint and Internet, 07 January 2013–11 January 2013, 07 October 2013–11 October 2013 Special course – MS Windows a MS Word, 14 January 2013–18 January 2013 Refresher course in MS Word, 20 May 2013–22 May 2013 Special Course – Statistics and data processing in MS Excel, 18 February 2013–22 February 2013, 11 March 2013–15 March 2013, 03 June 2013–07 June 2013 Molecular Pathology Specialized course – biosensors for detection of biological and chemical agents, 11 February 2013–11 February 2013, 11 November 2013–11 November 2013

22th Annual Report, 2013 – 83 WORKSHOPS, COURSES, RESIDENCIES AT THE FACULTY IN 2013

Multidisciplinary Studies Special course – Basis of pharmacology and self-treatment, 18 February 2013–21 February 2013 Special course – psychology and crisis interventions, management of difficult patients, 11 March 2013–13 March 2013, 14 October 2013–16 October 2013 Preparatory course for entrance examination, 03 June 2013–08 June 2013 Specialized course – Prevention of burn–out syndrom, 11 February 2013–13 February 2013 Specialized course – Dealing with extremely dangerous poisons, drugs and psychotropic substances, 04 February 2013–07 February 2013, 02 September 2013–05 September 2013, 21 October 2013–22 October 2013

22th Annual Report, 2013 – 84 INTERNATIONAL COOPERATION INTERNATIONAL COOPERATION

Cooperation at the military medical facility level

Brazil Faculdade de Farmácia, Salvador, Bahia Universidade Federal de Santa Catarina, Florianopolis Bulgaria Military Medical Academy, Sofia France Health Service and Army Research Center (C.R.S.S.A.), Grenoble School of the Health Service of the Armies of Lyon-Bron (E.S.S.A..), Lyon Germany Institute of Microbiology of Federal Armed Forces Medical Academy, Munich Institute of Pharmacology and Toxicology of Federal Armed Forces Medical Academy, Munich India Pandit Ravishankar Shukla University, Raipur Poland Military Institute of Hygiene and Epidemiology, Warsaw Serbia National Poison Control Centre of Military Medical Academy, Belgrade Slovakia Air Forces Hospital, Košice Central Military Hospital, Ružomberok Military Health Service, Bratislava Military Institute of Hygiene and Epidemiology, Bratislava Swedish Defence Research Agency, Dr. Artursson – Department of Threat Assessment, Division of NBC Defence, Umea The Division of Toxicology, TNO Prins Maurits Netherlands Laboratory, Rijswijk Turkey Gulhane Military Medical Academy, Ankara Ukraine Military Medical Corps, Kiev United Kingdom Defence Medical Services Training Centre Keogh Barracks in Aldershot, Ash Vale DRDC, Suffield

22th Annual Report, 2013 – 85 INTERNATIONAL COOPERATION

United States United States Defense Institute of International Legal Studies, Newport

Scientific cooperation with civilian institutions abroad (on the basis of individual agreements and joint projects) Austria Baxter, Vienna Center of Biomolecular Medicine & Pharmacology, Medical University of Vienna, Vienna Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna Belgium GlaxoSmithKline Biologicals, Rixensart Croatia Department of Microbiology and Parasitology, University of Rijeka, Rijeka Institute for Medical Research and Occupational Health, Zagreb France Aventis Pasteur MSD, Lyon Saint Louis Hospital, Paris Germany Department of Solid States Nuclear Physics, University of Leipzig, Leipzig Greece Universit of Crete, Iraklion Hungary Semmelweis University, Budapest Mongolia National Research Center for Infectious Diseases, Ministry of Health, Ulaanbaatar Norway Department of Nutrition, Medical Faculty, University of Oslo, Oslo Poland WIHE, Dr Zdanowski – Zakład Farmakologii i Toksykologii, Warsaw Portugal University of Coimbra, Department of Pharmacology, Coimbra Republic of Medicinal Science Division, Korea Research Korea Institute of Chemical Technology, Daejeon Russian M. V. Lomonosov Moscow State University, Federation Moskva Slovakia Institute of Experimental Oncology, Bratislava P. J. Šafárik University, Košice Slovak Medical University, Bratislava

22th Annual Report, 2013 – 86 INTERNATIONAL COOPERATION

Sweden University of Umea, Umea Switzerland Institute of Molecular Systems Biology, Zurich United Arab United Arab Emirates University, Prof. Lorke – Emirates Faculty of Medicine and Health Sciences – Department of Anatomy, Al-Ain United Kingdom Health Protection Agency, Porton Down University of St. Andrews, St. Andrews, Scotland United States Emory University, Department of Pathology, Atlanta Merck & Co., Inc, Whitehouse Station MMRHVLB/CCID/CDC, Atlanta United States University of Washington, Seattle Walter Reed Army Institute of Research, Silver Spring Wyeth, New Jersey

Participation in international projects and networks Belgium European Defence Agency, Brussels Sweden European Programme for Intervention Epidemiology Training, European Centre for Disease Prevention and Control, Stockholm Switzerland European Study Group on Nosocomial Infection, United States Indiana University, Bloomington, Indiana National Institute of Health, Food and Drug Administration, Centre of Biological Evaluation and Research, Bethesda, Maryland U. S. Army Medical Research Institute of Infectious Disease, Fort Detrick

Cooperation in the field of disaster medicine United Kingdom Royal Centre for Defence Medicine, Birmingham

22th Annual Report, 2013 – 87 INTERNATIONAL COOPERATION

Other expert commissions J. Bajgar – member of Editorial board of Archives of Hygiene and Industrial Toxicology R. Blanař – member of NATO RTO, Human Factors and Medicine – Exploratory Team “Information Technology and Models for Crisis Detection, Monitoring and Response“ R. Blanař – member of NATO (COMEDS-MMSOP) P. Boštík – chairman of RECOOP Association Select Committee P. Boštík – national coordinator for CBRN of Cap Tech ESM04 EDA P. Boštík – member of Regional Cooperation for Health Science and Technology P. Boštík – member of Editorial board of the Open Infectious Diseases Journal P. Boštík – member of the Association of UICC Fellows P. Boštík – member of the American Association of Immunologists (AAI) R. Chlíbek – member of C.O.P.E. – Consensus on Pertussis Booster Vaccination in Europe P. Boštík – member of the Federation of American Societies for Experimental Biology V. Boštíková – International Board for the Investigation and Control of Influenza and Other Epidemic Diseases V. Boštíková – member of Editorial board of Journal of Clinical Virology R. Chlíbek – member of GPI – Global Pertussis Initiative R. Chlíbek – member of NATO – Biological Medical Advisory Committee R. Chlíbek – member of NATO HFMP (Human Factor Medicine Panel) R. Chlíbek – member of Vaccination Advisory Board GlaxoSmithKline Biologicals R. Chlíbek – supervisor of CEVAG (Central European Vaccination Awareness Group) R. Chlíbek – member of Central and Eastern Europe Pertussis Awareness Group R. Chlíbek – member of C.O.P.E. – Consensus on Pertussis Booster Vaccination in Europe L. Jebavý – member of European Group for Blood and Marrow Transplantation L. Jebavý – member of Multinational Association of Supportive Care in Cancer L. Jebavý – member of European Study Group on Nosocomial Infections J. Kassa – member of NATO CBRN Medical Working Group

22th Annual Report, 2013 – 88 INTERNATIONAL COOPERATION

J. Kassa – member of Editorial board of Journal of Medical Chemical, Biological and Radiological Defence J. Kassa – member of Editorial board of Challenge Medical CBRN Defense International L. Klein – member of Editorial board of Annals of Burns and Fire Disasters L. Klein – member of NATO HFMP Z. Kročová– member of Bio EDEP Project 3 K. Kuča – member of Editorial board of Journal of Enzyme Inhibition and Medicinal Chemistry K. Kuča – member of Editorial board of Surgical Sciences (Scientific Research Publishing, Inc. USA) K. Kuča – member of Editorial board of Research in Pharmaceutical Biotechnology (Academic Journals) K. Kuča – member of Editorial board of ISRN Pharmaceutics (Hindawi) K. Kuča – member of Editorial board of World Journal of Metho- dology K. Kuča – member of Editorial board of World Journal of Translational Medicine K. Kuča – member of Editorial board of the Open Enzyme Inhibition Journal (Bentham) K. Kuča – consultant of Research Network Management Working Group; Cedar-Sinai RECOOP HST Consorcium K. Kuča – consultant of Guidepoint Global Advisors K. Kuča – member of Editorial board of Advances in Economics K. Kuča – member of Editorial board of Advances in Materials Science and Applications K. Kuča – member of Editorial board of Biochemistry & Physiology M. Pohanka – member of Editorial board of Journal of Biosafety M. Pohanka – member of Editorial board of Journal of Biosensors and Bioelectronics M. Pohanka – member of Editorial board of Journal of Cytoprotection and Damage Control M. Pohanka – member of Editorial board of Journal of Bioterrorism and Biodefense M. Pohanka – member of Editorial board of Journal of Obesity & Weight loss Therapy M. Pohanka – member of Editorial board of BioMed Research International M. Pohanka – member of Editorial board of International Journal of Drug Discovery M. Pohanka – member of Editorial board of International Journal of Health, Safety and Environments M. Pohanka – member of Editorial board of Journal of Clinical Trials

22th Annual Report, 2013 – 89 INTERNATIONAL COOPERATION

M. Pohanka – member of Editorial board of Microbial and Biochemical Technology M. Pohanka – member of Editorial board of Excellent Research Journal of Biotechnology R. Prymula – member of European Scientific Working Group on Influenza R. Prymula – member of Global Advisory Board on Pneumoccocal Vaccines R. Prymula – chairman of CEVAG (Central European Vaccine Advisory Group) R. Prymula – member of Management Board of ECDC R. Prymula – member of Steering Committee ECDC R. Prymula – member and secretariat of EVASG R. Prymula – member of ISW-TBE R. Prymula – member of SAATI J. Smetana – member of Rotavirus Vaccination Advisory Board GlaxoSmithKline Biologicals H. Střítecká – member of Editorial board of Journal of Obesity & Weight loss Therapy H. Střítecká – member of Editorial board Journal of Food Security – Science and Education Publishing (SciEP) J. Stulík – member of NATO HFM TG-186 J. Stulík– member of Editorial board of Frontiers in Cellular and Infection Microbiology M. Špliňo – International Board for the Investigation and Control of Influenza and Other Epidemic Diseases M. Špliňo – European Study Group on Nosocomial Infection R. Štětina – member of Working Group Asperillus (European Food Safety Authority)

22th Annual Report, 2013 – 90 SCIENTIFIC AND RESEARCH ACTIVITIES SCIENTIFIC AND RESEARCH ACTIVITIES

Completed full professorships Pazdiora Petr Institute of Epidemiology, Faculty of Medicine, Charles University in Pilsen area of specialization: Hygiene, Preventive Medicine and Epidemiology professor's lecture: Concept of scientific work and treining in the field of Hygiene, Preventive Medicine and Epidemiology

Completed associate professorships Růžek Daniel Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice area of specialization: Medical Microbiology habilitation thesis: Molecular aspects of epidemiology and pathogenesis of arboviral infections habilitation lecture: Incidence, diagnostics and taxonomy of hemorrhagic fevers with pulmonary-renal syndromes in the European Union countries Salavec Miroslav Department of Dermatology and Venereology, Charles University in Prague, Faculty of Medicine in Hradec Králové and Faculty Hospital Hradec Králové area of specialization: Hygiene, Preventive Medicine and Epidemiology habilitation thesis: Analysis of epidemiological situation of syphilis and gonorrhoea development in the Eastern Bohemia region in 1981–2011 habilitation lecture: Chlamydial infections – trends in icidence, diagnostics and treatment

22th Annual Report, 2013 – 91 SCIENTIFIC AND RESEARCH ACTIVITIES

Kročová Zuzana Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Králové area of specialization: Infection Biology habilitation thesis: Application of microbiological, immunological and molecular biological methods in a pathogenesis of F. tularensis microbe habilitation lecture: BIO DIM concept – current issue of detection, identification and monitoring of biological agents Tichý Aleš Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Králové area of specialization: Military Radiobiology habilitation thesis: Reparation of radiation-induced damage and its role in radio-sensitization of the cancer cells habilitation lecture: Radon and its environmental importance

Dissertation defences Korábečný Jan Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové study programmes: Pharmacy dissertation: Synthesis of tetrahydroacridine acetylcholinesterase inhibitors Fajfrová Jana Department of Hygiene, Faculty of Military Health Sciences, University of Defence, Hradec Králové study programmes: Preventive Medicine, Public Health Protection dissertation: Enhancement of fitness and health among career soldiers – metabolic syndrome and non-alcoholic fatty liver disease

22th Annual Report, 2013 – 92 SCIENTIFIC AND RESEARCH ACTIVITIES

Misík Jan Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové study programmes: Toxicology dissertation: In vivo skin decontamination – evaluation and optimization of new decontamination means Blechová Zuzana 1st Department of Infectious Diseases, 2nd Faculty of Medicine, Charles University in Prague, University Hospital Na Bulovce study programmes: Epidemiology dissertation: Epidemiology of community infections in children focused on pneumococci Frank Martin Department of Surgery, Faculty of Military Health Sciences, University of Defence, Hradec Králové study programmes: Military Surgery dissertation: Crossover external fixator of pelvic and acetabular fractures Fučíková Alena Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Králové study programmes: Infection Biology dissertation: Use of proteomic analysis in identification of potential biomarkers of cardiovascular diseases Pavliš Oto Military Health Institute, Těchonín study programmes: Toxicology dissertation: Substances affect cholinergic antiinflammatory pathway and their impact on the state of the organism during simultaneous infections

22th Annual Report, 2013 – 93 SCIENTIFIC AND RESEARCH ACTIVITIES

Dresler Jiří Central Military Health Institute, Prague study programmes: Infection Biology dissertation: Identification of new potential virulence factors of Francisella tularensis

22th Annual Report, 2013 – 94 REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FACULTY OF MILITARY HEALTH SCIENCES IN 2013

THE INTERNAL GRANT AGENCY OF THE CZECH REPUBLIC HEALTH SERVICE

Principal investigators Kamil Kuča (NT12062) Preparation and biological evaluation of new therapeutics against to pesticides Jiří Stulík (NT13721) Targeted proteomic analysis in hypertrophic cardiomyopathy Juraj Lenčo (NT13599) Characterization of the diagnostic potential of native polypeptides in amniotic fluid Miroslav Procházka (NT14460) Prehospital emergency care efficiency

Co-investigators Daniel Jun (NT12062) Preparation and biological evaluation of new therapeutics against to pesticides Miroslav Pohanka (NT12062) Preparation and biological evaluation of new therapeutics against to pesticides Jana Žďárová Karasová (NT12062) Preparation and biological evaluation of new therapeutics against to pesticides Alena Fučíková (NT13721) Targeted proteomic analysis in hypertrophic cardiomyopathy Jiří Páral (NT13726) Parametric monitoring the quality of TME as a tool to reduce local recurrences after surgery for rectal cancer

22th Annual Report, 2013 – 95 THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

Ladislav Jebavý (NT11299) Quality of life measurement and its influence to overall survival in hematopoietic stem cell transplantation in CZ Tomáš Halajčuk (NT14460) Prehospital emergency care efficiency Zdeněk Hrstka (NT14460) Prehospital emergency care efficiency Bruno Ježek (NT14460) Prehospital emergency care efficiency Jan Vaněk (NT14460) Prehospital emergency care efficiency Jaroslav Pejchal (NT13413) Determination of apoptosis in the bioptic samples taken from the colon Zdeněk Šubrt (NT13660) Quality evaluation of multimodal treatment in patients with colorectal liver metastatic disease: Mutlicentric study within Czech complex oncology centres

DEFENSE THREAT REDUCTION AGENCY

Principal investigators Jiří Stulík (D-CZ-10-0001) Identification of novel Francisella tularensis targets for subunit vaccine development

THE GLAXOSMITHKLINE BIOLOGICALS CO.

Principal investigators Roman Chlíbek (113077 (ZOSTER-022)) A phase III, randomized, observer-blind, placebo controlled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety and immunogenicity of GSK Biologicals’ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2- month schedule in adults aged 70 years and older

22th Annual Report, 2013 – 96 REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

Roman Chlíbek (114825, Zoster-024) An open, phase II long term extension study to evaluate the immune responses to and safety of GSK Biologicals’ candidate herpes zoster vaccine (gE/AS01B) at Months 48, 60 and 72 post- vaccination in healthy subjects aged 60 years of age and older Roman Chlíbek (115790 EPI-Pertussis) Epidemiological observational prospective cohort study to evaluate the sero-prevalence of Bordetella pertussis in adults in the Czech Republic Roman Chlíbek (110390 (ZOSTER-006)) A phase III, randomized, observer-blind, placebocontrolled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety, and immunogenicity of GSK Biologicals´ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2-month schedule in adults aged 50 years and older

Co-investigators Jan Smetana (113077 (ZOSTER-022)) A phase III, randomized, observer-blind, placebo controlled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety and immunogenicity of GSK Biologicals’ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2- month schedule in adults aged 70 years and older Jan Smetana (115790 EPI-Pertussis) Epidemiological observational prospective cohort study to evaluate the sero-prevalence of Bordetella pertussis in adults in the Czech Republic Jan Smetana (110390 (ZOSTER-006)) A phase III, randomized, observer-blind, placebocontrolled, multicentre, clinical vaccination trial to assess the prophylactic efficacy, safety, and immunogenicity of GSK Biologicals´ herpes zoster gE/AS01B vaccine when administered intramuscularly on a 0, 2-month schedule in adults aged 50 years and older Jan Smetana (114825, Zoster-024) An open, phase II long term extension study to evaluate the immune responses to and safety of GSK Biologicals’ candidate herpes zoster vaccine (gE/AS01B) at Months 48, 60 and 72 post- vaccination in healthy subjects aged 60 years of age and older

22th Annual Report, 2013 – 97 THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS THE CZECH REPUBLIC MINISTRY OF INTERNAL AFFAIRS

Principal investigators Zuzana Kročová (VF20122015024) New technologies for identification and typing of biological agents

Co-investigators Pavel Boštík (VF20122015024) New technologies for identification and typing of biological agents Irena Hanovcová (VF20122015024) New technologies for identification and typing of biological agents Daniel Jun (VF20122015024) New technologies for identification and typing of biological agents Aleš Macela (VF20122015024) New technologies for identification and typing of biological agents

GRANT AGENCY OF CHARLES UNIVERSITY

Co-investigators Aleš Tichý (GAUK 2013) Radio-sensibilization of MOLT-4 and SAOS-2 cell lines by DNA repair inhibition: Phosphoproteomic analysis of irradiated cancer cells

22th Annual Report, 2013 – 98 REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

THE ROCHE CO.

Principal investigators Vanda Boštíková (VZV) Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System

Co-investigators

Jan Smetana (VZV) Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System Pavel Boštík (VZV) Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System Lenka Kaislerová (VZV) Whole varicella-zoster virus (VZV) genome sequencing of individual wild type and vaccine strains using GS Junior Benchtop System

THE NOVARTIS

Principal investigators Roman Prymula (V72P12E2, EUDRACT No 2011-004931-30) A phase 3, open label, multi- center, extension study to assess antibody persistence and response to a third dose of Novartis meningococcal B recombinant vaccine in 4-year- old children who previously participated in study V72P12E1

Co-investigators Roman Chlíbek (V72P12E2, EUDRACT No 2011-004931-30) A phase 3, open label, multi- center, extension study to assess antibody persistence and response to a third dose of Novartis meningococcal B recombinant vaccine in 4-year- old children who previously participated in study V72P12E1

22th Annual Report, 2013 – 99 THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

THE CZECH REPUBLIC GRANT AGENCY

Principal investigators Zuzana Kročová (GAP302/11/1631) The role of B cells during natural and adaptive phase of immune response against Francisella tularensis infection in mice Kamil Kuča (GAP303/11/1907) Novel inhibitors of acetylcholinesterase derived from 7- MEOTA – potential Alzheimer´s disease drugs Pavel Boštík (GAP304/10/1161) The role of virus associated cellular proteins in T- lymphocyte dysfunction Aleš Tichý (GPP206/12/P338) Phosphoproteomic analysis of leukaemic cells after irradiation Jiří Kassa (GAP303/12/0611) Neurobehavioral evaluation of potential Alzhemiers disease drugs

Co-investigators Pavel Řehulka (GAP304/10/1161) The role of virus associated cellular proteins in T- lymphocyte dysfunction Vanda Boštíková (GAP304/10/1161) The role of virus associated cellular proteins in T- lymphocyte dysfunction Jaroslav Pejchal (GAP304/10/1161) The role of virus associated cellular proteins in T- lymphocyte dysfunction Zuzana Kročová (GAP304/10/1161) The role of virus associated cellular proteins in T- lymphocyte dysfunction Jan Misík (GAP303/12/0611) Neurobehavioral evaluation of potential Alzhemiers disease drugs

22th Annual Report, 2013 – 100 REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

Kamil Kuča (GAP303/12/0611) Neurobehavioral evaluation of potential Alzhemiers disease drugs Kamil Musílek (GAP303/12/0611) Neurobehavioral evaluation of potential Alzhemiers disease drugs Jana Žďárová Karasová (GAP303/12/0611) Neurobehavioral evaluation of potential Alzhemiers disease drugs

THE MINISTRY OF EDUCATION, YOUTH AND SPORTS

Principal investigators Pavel Boštík (LH11019) Correlation of expression of KIR alleles in NK cells in GALT and disease progression in SIV non-human primate model of AIDS Miroslav Pohanka (LH11023) Improvement of vaccination efficacy by cholinergic anti- inflammatory pathway Jiří Stulík (7AMB12GR038) Proteome analysis of the interaction of host cell with intracellular pathogen

Co-investigators Vanda Boštíková (LH11019) Correlation of expression of KIR alleles in NK cells in GALT and disease progression in SIV non-human primate model of AIDS Kamil Kuča (LH11023) Improvement of vaccination efficacy by cholinergic anti- inflammatory pathway Pavel Boštík (LH11023) Improvement of vaccination efficacy by cholinergic anti- inflammatory pathway

22th Annual Report, 2013 – 101 THE REVIEW OF RESEARCH PROJECTS CARRIED OUT AT THE FMHS

Michal Pavlík (LH11023) Improvement of vaccination efficacy by cholinergic anti- inflammatory pathway Martina Hrabinová (LH11023) Improvement of vaccination efficacy by cholinergic anti- inflammatory pathway

THE MINISTRY OF INDUSTRY AND TRADE

Co-investigators Lenka Hernychová (FR-TI1/292) Molecular diagnostics of bacterial antigens

RESEARCH AIMS

Jiří Kassa A long-term organization development plan 1011 – Health problems of the weapons of mass destruction Jiří Páral A long-term organization development plan 1011 – Clinical fields

22th Annual Report, 2013 – 102 ARTICLES IN JOURNAL WITH IMPACT FACTOR ARTICLES IN JOURNALS WITH IMPACT FACTOR

1. BABICOVÁ, A., HAVLÍNOVÁ, Z., HROCH, M., ŘEZÁŘOVÁ, M., PEJCHAL, J., VÁVROVÁ, J., CHLÁDEK, J. In vivo study of radioprotective effect of NO-synthase inhibitors and acetyl-L- carnitine. Physiological Research. 2013, 62(6), 701–710. ISSN 0862- 8408. IF 1.531 2. BHATTACHARJEE, AK., MUSÍLEK, K., KUČA, K. In silico pharmacophore modeling on known pyridinium oxime reactivators of cyclosarin (GF) inhibited AChE to aid discovery of potential, more efficacious novel non-oxime reactivators. Current Computer-Aided Drug Design. 2013, 9(3), 402–411. ISSN 1573-4099. IF 1.54 3. BOŠTÍKOVÁ, V., CHLÍBEK, R., SALAVEC, M., SMETANA, J., SLEHA, R., ŠPLIŇO, M., BOŠTÍK, P. Molecular biological and epidemiological characteristics of varicella virus. Epidemiologie, mikrobiologie, imunologie. 2013, 62(4), 148–152. ISSN 1210-7913. IF 0.306 4. BUREŠ, J., KVĚTINA, J., PAVLÍK, M., KUNEŠ, M., KOPÁČOVÁ, M., REJCHRT, S., JUN, D., HRABINOVÁ, M., KUČA, K., TACHECÍ, I. Impact of paraoxon followed by acetylcholinesterase reactivator HI-6 on gastric myoelectric activity in experimental pigs. Neuroendocrinology Letters. 2013, 34(suppl. 2), 79–83. ISSN 0172-780X. IF 0.932 5. CAHLÍKOVÁ, L., HRABINOVÁ, M., KULHÁNKOVÁ, A., BENEŠOVÁ, N., CHLEBEK, J., JUN, D., NOVÁK, Z., MACÁKOVÁ, K., KUNEŠ, J., KUČA, K., OPLETAL, L. Alkaloids from chlidanthus fragrans and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase activities. Natural Product Communications. 2013, 8(11), 1541–1544. ISSN 1934-578X. IF 0.956 6. ČEČKA, F., JON, B., ŠUBRT, Z., FERKO, A. Clinical and economic consequences of pancreatic fistula after elective pancreatic resection. Hepatobiliary & Pancreatic Diseases International. 2013, 12(5), 533– 538. ISSN 1499-3872. IF 1.259 7. ČEČKA, F., FERKO, A., JON, B., ŠUBRT, Z., KAŠPAROVÁ, P., REPÁK, R. Pancreatic castleman disease treated with laparoscopic distal pancreatectomy. Hepatobiliary & Pancreatic Diseases International. 2013, 12(3), 332–334. ISSN 1499-3872. IF 1.259 8. ČERVENÝ, L., STRAŠKOVÁ, A., DAŇKOVÁ, V., HÄRTLOVÁ, A., ČEČKOVÁ, M., ŠTAUD, F., STULÍK, J. Tetratricopeptide repeat motifs in the world of bacterial pathogens: role in virulence mechanisms.

22th Annual Report, 2013 – 103 ARTICLES IN JOURNALS WITH IMPACT FACTOR

Infection and Immunity. 2013, 81(3), 629–635. ISSN 0019-9567. IF 4.074 9. ČMIELOVÁ, J., HAVELEK, R., KOHLEROVÁ, R., SOUKUP, T., BRUČKOVÁ, L., SUCHÁNEK, J., VÁVROVÁ, J., MOKRÝ, J., ŘEZÁČOVÁ, M. The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation. International Journal of Radiation Biology. 2013, 89(7), 501–511. ISSN 0955-3002. IF 1.895 10. DRTINOVÁ, L., POHANKA, M. Application of huperzine A for Alzheimer disease treatment. Chemické listy. 2013, 107(1), 12–15. ISSN 0009- 2770. IF 0.453 11. FABRIK, I., HÄRTLOVÁ, A., ŘEHULKA, P., STULÍK, J. Serving the new masters – dendritic cells as hosts for stealth intracellular bacteria. Cellular Microbiology. 2013, 15(9), 1473–1483. ISSN 1462-5814. IF 4.811 12. FIKROVÁ, P., ŠTĚTINA, R., HRNČIARIK, M., ŘEHÁČEK, V., HRONEK, M., ZADÁK, Z. Detection of DNA crosslinks in peripheral lymphocytes isolated from patients treated with platinum derivates using modified comet assay. Neoplasma. 2013, 60(4), 413–418. ISSN 0028-2685. IF 1.574 13. FRANC, V., ŘEHULKA, P., RAUS, M., STULÍK, J., NOVAK, J., RENFROW, MB., SEBELA, M. Elucidating heterogeneity of IgA1 hinge- region O-glycosylation by use of MALDI-TOF/TOF mass spectrometry: Role of cysteine alkylation during sample processing. Journal of Proteomics. 2013, 92(54), 299–312. ISSN 1874-3919. IF 4.088 14. FRANC, V., ŘEHULKA, P., MEDDA, R., PADDIGLIA, A., FLORIS, G., ŠEBELA, M. Analysis of the glycosylation pattern of plant copper amine oxidases by MALDI-TOF/TOF MS coupled to a manual chromatographic separation of glycans and glycopeptides. Electrophoresis. 2013, 34(16), 2357–2367. ISSN 0173-0835. IF 3.261 15. HARTMANOVÁ, T., TAMBOR, V., LENČO, J., STAAB-WEIJNITZ, C., MASER, E., WSÓL, V. S-nitrosoglutathione covalently modifies cysteine residues of human carbonyl reductase 1 and affects its activity. Chemico-Biological Interactions. 2013, 202(1–3), 136–145. ISSN 0009- 2797. IF 2.967 16. HAVELEK, R., SOUKUP, T., ČMIELOVÁ, J., SEIFRTOVÁ, M., SUCHÁNEK, J., VÁVROVÁ, J., MOKRÝ, J., MUTHNÁ, D., ŘEZÁČOVÁ, M. Ionizing radiation induces senescence and differentiation of human dental pulp stem cells. Folia biologica (Prague). 2013, 59(5), 188–197. ISSN 0015-5500. IF 1.219

22th Annual Report, 2013 – 104 ARTICLES IN JOURNAL WITH IMPACT FACTOR

17. HLÚBIK, J., STŘÍTECKÁ, H., HLÚBIK, P. Bioelectrical impedance analysis or basic anthropometrical parameters for evaluating weight loss success? Central European Journal of Medicine. 2013, 8(5), 565– 570. ISSN 1895-1058. IF 0.262 18. HORÁČEK, J., JEBAVÝ, L., VAŠATOVÁ, M., PUDIL, R., TICHÝ, M., JAKL, M., MALÝ, J. Glycogen phosphorylase BB as a potential marker of cardiac toxicity in patients treated with anthracyclines for acute leukemia. Bratislava Medical Journal-Bratislavske Lekarske Listy. 2013, 114(12), 708–710. ISSN 0006-9248. IF 0.472 19. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., JEBAVÝ, L., ŽÁK, P. Multi- analytical evaluation of serum levels of cytokines and adhesion molecules in patients treated for acute myeloid leukemia using biochip array technology. Biomedical Papers. 2013, 157(4), 277–279. ISSN 1213-8118. IF 0.99 20. CHLÍBEK, R., BAYAS, J., COLLINS, H., DE LA PINTA, M., LEDENT, E., MOLS, J., HEINEMAN, T. Safety and immunogenicity of an AS01 adjuvanted varicella-zoster virus subunit candidate vaccine against herpes zoster in adults (50 years of age). Journal of Infectious Diseases. 2013, 12(208), 1953–1961. ISSN 0022-1899. IF 5.848 21. CHUDOBOVÁ, D., DOBEŠ, J., NEJDL, L., MAŠKOVÁ, D., RODRIGO, M., BRANISLAV-RUTTKAY, N., KRYŠTOFOVÁ, O., KYNICKÝ, J., KONEČNÁ, M., POHANKA, M., HUBÁLEK, J., ZEHNÁLEK, J., KLEJDUS, B., KIZEK, R., ADAM, V. Oxidative stress in staphylococcus aureus treated with silver(I) Ions revealed by spectrometric and voltammetric assays. International Journal of Electrochemical Science. 2013, 8(4), 4422–4440. ISSN 1452-3981. IF 0 22. JAKL, M., SEVCIK, R., CERAL, J., FATOROVA, I., HORÁČEK, J., VOJACEK, J. Mean platelet volume and platelet count: overlooked markers of high on-treatment platelet reactivity and worse outcome in patients with acute coronary syndrome. Anadolu Kardiyoloji Dergisi- The Anatolian Journal of Cardiology. 2013, 13(9), 4803–4804. ISSN 1302-8723. IF 0.715 23. JURÍKOVÁ, T., BALLA, S., SOCHOR, J., POHANKA, M., MLCEK, J., BARON, M. Flavonoid profile of saskatoon berries (Amelanchier alnifolia nutt.) and their health promoting effects. Molecules. 2013, 18(10), 12571–12586. ISSN 1420-3049. IF 2.428 24. KACEROVSKÝ, M., TAMBOR, V., VAJRYCHOVÁ, M., LENČO, J., HORNYCHOVÁ, H., MUSILOVÁ, I., MENON, R. Amniotic fluid myeloperoxidase in pregnancies complicated by preterm prelabor rupture of membranes. Journal of Maternal-Fetal & Neonatal Medicine. 2013, 26(5), 463–468. ISSN 1476-7058. IF 1.518

22th Annual Report, 2013 – 105 ARTICLES IN JOURNALS WITH IMPACT FACTOR

25. KASSA, J., ŽĎÁROVÁ KARASOVÁ, J., ŠEPSOVÁ, V. A comparison of the reactivating efficacy of a novel bispyridinium oxime K203 with currently available oximes in VX agent-poisoned rats. Journal of Enzyme Inhibition and Medicinal Chemistry. 2013, 28(4), 753–757. ISSN 1475-6366. IF 1.495 26. KASSA, J., MISÍK, J., ŽĎÁROVÁ KARASOVÁ, J. Evaluation of the potency of two novel bispyridinium oximes (K456, K458) in comparison with the oxime K203 and trimedoxime to counteract tabun-induced neurotoxicity in rats. Basic & Clinical Pharmacology & Toxicology. 2013, 113(3), 201–208. ISSN 1742-7835. IF 2.124 27. KASSA, J., ŠEPSOVÁ, V., MUSÍLEK, K., HOROVÁ, A. The evaluation of the reactivating and therapeutic efficacy of three novel bispyridinium oximes (K454, K456, K458) in comparison with the oxime K203 and trimedoxime in tabun-poisoned rats and mice. Toxicology Mechanisms and Methods. 2013, 23(2), 94–98. ISSN 1537-6516. IF 1.367 28. KASSA, J., ŽĎÁROVÁ KARASOVÁ, J., KREJČIOVÁ, M. Therapeutic efficacy of a novel bispyridinium oxime K203 and commonly used oximes (HI-6, obidoxime, trimedoxime, methoxime) in soman-poisoned male rats and mice. Journal of Applied Biomedicine - print. 2013, 11(1), 7–13. ISSN 1214-021X. IF 0.978 29. KOMLÓOVÁ, M., HOROVÁ, A., HRABINOVÁ, M., JUN, D., DOLEŽAL, M., VINŠOVÁ, J., KUČA, K., MUSÍLEK, K. Preparation, in vitro evaluation and molecular modelling of pyridinium– quinolinium/isoquinolinium non-symmetrical bisquaternary cholinesterase inhibitors. Bioorganic & Medicinal Chemistry Letters. 2013, 23(24), 6663–6666. ISSN 0960-894X. IF 2.338 30. KORÁBEČNÝ, J., JANOVEC, L., MUSÍLEK, K., ZEMEK, F., HOROVÁ, A., NEPOVÍMOVÁ, E., DOLEŽAL, R., OPLETALOVÁ, V., HROUDOVÁ, J., FIŠAR, Z., JUNG, Y., KUČA, K. Comparison of novel tacrine and 7- MEOTA derivatives with aromatic and alicyclic residues : synthesis, biological evaluation and docking studies. Letters in Organic Chemistry. 2013, 10(4), 291–297. ISSN 1570-1786. IF 0.667 31. KUČA, K., MUSÍLEK, K., JUN, D., ŽĎÁROVÁ KARASOVÁ, J., SOUKUP, O., PEJCHAL, J., HRABINOVÁ, M. Structure-activity relationship for the reactivators of acetylcholinesterase inhibited by nerve agent VX. Medicinal Chemistry. 2013, 9(5), 689–693. ISSN 1573- 4064. IF 1.373 32. KUČA, K., JUN, D., MUSÍLEK, K., POHANKA, M., ŽĎÁROVÁ KARASOVÁ, J., SOUKUP, O. Prophylaxis and post-exposure treatment of intoxications caused by nerve agents and organophosphorus

22th Annual Report, 2013 – 106 ARTICLES IN JOURNAL WITH IMPACT FACTOR

pesticides. Mini Reviews in Medicinal Chemistry. 2013, 13(14), 2102– 2115. ISSN 1389-5575. IF 2.865 33. KUNZE, U., ASPOCK, H., AVDIČOVÁ, M., BOHM, G., BRETSCHNEIDER, R., BROCHIER, B., CHITIMIA, L., CHLÍBEK, R., CHREN, M., PRYMULA, R. Tick-borne encephalitis – a notifiable disease: report of the 15th Annual Meeting of the International Scientific Working Group on Tisk-Borne Encephalitis (ISW-TBE). Ticks and Tick- Borne Diseases. 2013, 4(5), 363–365. ISSN 1877-959X. IF 2.353 34. LORKE, DE., HASAN, MY., NURULAIN, SM., SHAFIULLAH, M., KUČA, K., PETROIANU, GA. Acetylcholinesterase inhibitors as pretreatment before acute exposure to organophosphates: assessment using methyl-paraoxon. CNS & Neurological Disorders- Drug Targets. 2013, 11(8), 1052–1060. ISSN 1871-5273. IF 3.769 35. LUKÁŠOVÁ, E., KOŘISTEK, Z., KLABUSAY, M., ONDŘEJ, V., GRIGORYEV, S., BAČÍKOVÁ, A., ŘEZÁČOVÁ, M., FALK, M., VÁVROVÁ, J., KOHÚTOVÁ, V., KOZUBEK, S. Granulocyte maturation determines ability to release chromatin NETs and loss of DNA damage response; these properties are absent in immature AML granulocytes. Biochimica et Biophysica Acta-Molecular Cell Research. 2013, 1833(3), 767–779. ISSN 0167-4889. IF 4.808 36. MAZAN, M., BLANCO, N., KOVACOVA, K., FIRAKOVA, Z., ŘEHULKA, P., FARKAS, V., ARROY, J. A novel fluorescence assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases. Biochemical Journal. 2013, 455(47), 307–318. ISSN 0264-6021. IF 4.654 37. MÉSZNER, Z., ANCA, I., ANDRÉ, F., CHLÍBEK, R., ČIŽMAN, M., GRZESIOWSKI, P., MANGAROV, A., POKORN, M., PRYMULA, R., RICHTER, D., SALMAN, N., ŠIMURKA, P., TAMM, E., TEŠOVIC, G., URBANCIKOVA, I., USONIS, V., ZAVADSKA, D., Rotavirus vaccination in central Europe. Journal of Pediatric Gastroenterology and Nutrition. 2013, 56(6), 586–596. ISSN 0277-2116. IF 2.196 38. MIKEŠ, J., KOVAL, J., JENDŽELOVSKÝ, R., MIKEŠOVÁ, L., ŠINKOROVÁ, Z., FEDOROČKO, P. Procedure for improved cleaning of FACSAria cuvette flow cell. Cytometry Part A. 2013, 83A(6), 523–527. ISSN 1552-4922. IF 3.711 39. MISÍK, J., PAVLÍKOVÁ, R., CABAL, J., KUČA, K. Percutaneous toxicity and decontamination of soman, VX, and paraoxon in rats using detergents. Archives of Industrial Hygiene and Toxicology. 2013, 64(2), 211–217. ISSN 0004-1254. IF 0.674 40. MISÍK, J., JOŠT, P., PAVLÍKOVÁ, R., VODÁKOVÁ, E., CABAL, J., KUČA, K. A comparison of decontamination effects of commercially

22th Annual Report, 2013 – 107 ARTICLES IN JOURNALS WITH IMPACT FACTOR

available detergents in rats preexposed to topical sulphur mustard. Cutaneous and Ocular Toxicology. 2013, 32(2), 135–139. ISSN 1556- 9527. IF 1.044 41. NOVOTNÁ, E., TICHÝ, A., PEJCHAL, J., LUKÁŠOVÁ, E., ŠALOVSKÁ, B., VÁVROVÁ, J. DNA-dependent protein kinase and its inhibition in support of radiotherapy. International Journal of Radiation Biology. 2013, 89(6), 416–423. ISSN 0955-3002. IF 1.895 42. ORDRONNEAU, L., CARELLA, A., POHANKA, M., SIMONATO, J. Chromogenic detection of Sarin by discolouring decomplexation of a metal coordination complex. Chemical Communications. 2013, 49(79), 8946–8948. ISSN 1359-7345. IF 6.378 43. PÁVKOVÁ, I., BRYCHTA, M., STRAŠKOVÁ, A., SCHMIDT, M., MACELA, A., STULÍK, J. Comparative proteome profiling of host- pathogen interactions: insights into the adaptation mechanisms of Francisella tularensis in the host cell environment. Applied Microbiology and Biotechnology. 2013, 97(21), 10103–10115. ISSN 0175-7598. IF 3.689 44. PAVLÍČEK, O., POLÁŠEK, M., FOLTÝN, M., CABAL, J. Automated detection of organophosphate warfare gases (nerve agents) in air based on micro-SIA - lab-on-valve system. Journal of Applied Biomedicine - print. 2013, 11(1), 27–32. ISSN 1214-021X. IF 0.978 45. PAVLÍK, V., FAJFROVÁ, J., SLOVÁČEK, L., DRAHOKOUPILOVÁ, E. The role of sibutramine in weight reduction. Bratislava Medical Journal- Bratislavske Lekarske Listy. 2013, 114(3), 155–157. ISSN 0006-9248. IF 0.472 46. PAVLÍK, V., FAJFROVÁ, J., DRAHOKOUPILOVÁ EVA, C. The effect of orlistat on body weight in obese Czech adults. Central European Journal of Medicine. 2013, 8(5), 553–557. ISSN 1895-1058. IF 0.262 47. PETROIANU, GA., NURULAIN, SM., SHAFIULLAH, M., HASAN, MY., KUČA, K., LORKE, DE. Usefulness of administration of non- organophosphate cholinesterase inhibitors before acute exposure to organophosphates: assessment using paraoxon. Journal of Applied Toxicology. 2013, 33(9), 894–900. ISSN 0260-437X. IF 2.597 48. PIKULA, J., HÁJKOVÁ, P., BANĎOUCHOVÁ, H., BEDNÁŘOVÁ, I., ADAM, V., BEKLOVÁ, M., KRÁL, J., ONDRÁČEK, K., OSIČKOVÁ, J., POHANKA, M., SEDLÁČKOVÁ, J., SKOCHOVÁ, H., SOBOTKA, J., TREML, F., KIZEK, R. Lead toxicosis of captive vultures: case description and responses to chelation therapy. BMC Veterinary Research. 2013, 9(11), Art. No. 11. ISSN 1746-6148. IF 1.861

22th Annual Report, 2013 – 108 ARTICLES IN JOURNAL WITH IMPACT FACTOR

49. POHANKA, M. Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates. Analytical Letters. 2013, 46(8), 1306–1315. ISSN 0003-2719. IF 0.965 50. POHANKA, M. Alzheimer´s disease and oxidative stress. A review. Current Medicinal Chemistry. 2013, 21(3), 356–364. ISSN 0929-8673. IF 4.07 51. POHANKA, M., ŠTĚTINA, R., SVOBODOVÁ, H., RUTTKAY- NEDECKÝ, B., JÍLKOVÁ, M., SOCHOR, J., SOBOTKA, J., ADAM, V., KIZEK, R. Sulfur mustard causes oxidative stress and depletion of antioxidants in muscles, livers, and kidneys of Wistar rats. Drug and Chemical Toxicology. 2013, 36(3), 270–276. ISSN 0148-0545. IF 1.293 52. POHANKA, M. Impact of melatonin on immunity. Central European Journal of Medicine. 2013, 8(4), 369–376. ISSN 1895-1058. IF 0.262 53. POHANKA, M., DRTINOVÁ, L. Spectrophotometric methods based on 2,6-dichloroindophenol acetate and indoxylacetate for butyrylcholinesterase activity assay in plasma. Talanta. 2013, 106(1), 281–285. ISSN 0039-9140. IF 3.498 54. POHANKA, M. Biosensors based on cholinesterases. Chemické listy. 2013, 107(2), 121–125. ISSN 0009-2770. IF 0.453 55. POHANKA, M., FUSEK, J., ADAM, V., KIZEK, R. Carbofuran assay using gelatin based biosensor with acetylcholinesterase as a recognition element. International Journal of Electrochemical Science. 2013, 8(1), 71–79. ISSN 1452-3981. 56. POHANKA, M. HI-6 modulates immunization efficacy in a BALB/c mouse model. Environmental Toxicology and Pharmacology. 2013, 36(3), 801–806. ISSN 1382-6689. IF 2.005 57. POHANKA, M., DOBEŠ, P. Caffeine inhibits acetylcholinesterase but not butyrylcholinesterase. International Journal of Molecular Sciences. 2013, 14(5), 9873–9882. ISSN 1422-0067. IF 2.464 58. POHANKA, M., PAVLIŠ, O. Tacrine can suppress immunity response to tularemia in BALB/c mouse model. Journal of Applied Biomedicine - print. 2013, 11(3), 187–193. ISSN 1214-021X. IF 0.978 59. POHANKA, M. Butyrylcholinesterase as a biochemical marker, a review. Bratislava Medical Journal-Bratislavske Lekarske Listy. 2013, 114(12), 726–734. ISSN 0006-9248. IF 0.472 60. POHANKA, M. Role of oxidative stress in infectious diseases. A review. Folia microbiologica. 2013, 58(6), 503–513. ISSN 0015-5632. IF 0.791

22th Annual Report, 2013 – 109 ARTICLES IN JOURNALS WITH IMPACT FACTOR

61. POHANKA, M., ADAM, V., KIZEK, R. Acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents. Sensors. 2013, 13(9), 11498–11506. ISSN 1424-8220. IF 1.953 62. POHANKA, M. Cholinesterases in biorecognition and biosensors construction: a review. Analytical Letters. 2013, 46(12), 1849–1868. ISSN 0003-2719. IF 0.965 63. POHANKA, M., PAVLIŠ, O., PIKULA, J. Tacrine alters antibodies level in Francisella tularensis-infected mice. Neuroendocrinology Letters. 2013, 34(S2), 134–137. ISSN 0172-780X. IF 0.932 64. SALAVEC, M., BOŠTÍKOVÁ, V., BLEHOVÁ, Z., SLEHA, R., PLÍŠEK, S., BOŠTÍK, P. Neurological complications dengue – a potential threat to Central Europe. Česká a slovenská neurologie a neurochirurgie. 2013, 76(5), 592–595. ISSN 1210-7859. IF 0.366 65. SALAVEC, M., BOŠTÍKOVÁ, V., VAŇÁSKOVÁ, Z., SMETANA, J., SLEHA, R., COUFALOVÁ, M., PLÍŠEK, S., ŠPLIŇO, M., BOŠTÍK, P. Treponema pallidum, subspecies pallidum. Epidemiologie, mikrobiologie, imunologie. 2013, 2013(3), 91–99. ISSN 1210-7913. IF 0.306 66. SCHMIDT, M., KLIMENTOVÁ, J., ŘEHULKA, P., STRAŠKOVÁ, A., ŠPIDLOVÁ, P., SZOTÁKOVÁ, B., STULÍK, J., PÁVKOVÁ, I. Francisella tularensis subsp. holarctica DsbA homologue: a thioredoxin-like protein with chaperone function. Microbiology -SGM. 2013, 159(11), 2364– 2374. ISSN 1350-0872. IF 2.852 67. SLEHA, R., BOŠTÍKOVÁ, V., SALAVEC, M., MOSIO, P., KUSÁKOVÁ, E., KUKLA, R., MAZUROVÁ, J. Bacterial infection as a cause of infertility in humans. Epidemiologie, mikrobiologie, imunologie. 2013, 62(1), 26–32. ISSN 1210-7913. IF 0.306 68. SMETANA, J., MACHAČ, J., PRÁŠIL, P., BOŠTÍKOVÁ, V., PLÍŠEK, S., KOSINA, P., HERMANOVÁ, Z., SALAVEC, M., JANDURA, J., CHLÍBEK, R. Spondylodiscitis wiht abscess formation in paravertebral muscles due to Streptococcus suis – a case report. Česká a slovenská neurologie a neurochirurgie. 2013, 76(6), 769–772. ISSN 1210-7859. IF 0.366 69. SNOPKOVÁ, S., POHANKA, M., POLÁK, P., HAVLÍČKOVÁ, K., JARKOVSKÝ, J., MOULIS, M., STROBLOVÁ, H., HUSA, P. Acute toxoplasmosis – etiological factor for development of Hodgkin´s lymphoma? Scandinavian Journal of Infectious Diseases. 2013, 45(12), 953–956. ISSN 0036-5548. IF 1.706 70. SOCHOR, J., DOBEŠ, J., KRYŠTOFOVÁ, O., RUTTKAY-NEDECKÝ, B., BABULA, P., POHANKA, M., JUŘÍKOVÁ, T., ZÍTKA, O., ADAM, V.,

22th Annual Report, 2013 – 110 ARTICLES IN JOURNAL WITH IMPACT FACTOR

KLEJDUS, B., KIZEK, R. Electrochemistry as a tool for studying antioxidant properties. International Journal of Electrochemical Science. 2013, 8(6), 8464–8489. ISSN 1452-3981. IF 0.0 71. SOUKUP, O., JUN, D., TOBIN, G., KUČA, K. The summary on non- reactivation cholinergic properties of oxime reactivators: the interaction with muscarinic and nicotinic receptors. Archives of Toxicology. 2013, 87(4), 711–719. ISSN 0340-5761. IF 5.215 72. SOUKUP, O., JUN, D., ŽĎÁROVÁ KARASOVÁ, J., PATOČKA, J., MUSÍLEK, K., KORÁBEČNÝ, J., KRUSEK, J., KANIAKOVÁ, M., ŠEPSOVÁ, V., MANDÍKOVÁ, J., TREJTNAR, F., POHANKA, M., DRTINOVÁ, L., PAVLÍK, M., TOBIN, G., KUČA, K. A resurrection of 7- MEOTA: a comparison with tacrine. Current Alzheimer Research. 2013, 10(8), 893–906. ISSN 1567-2050. IF 3.676 73. STRAKOVA, E., BOBEK, J., ZIKOVA, A., ŘEHULKA, P., BENADA, O., ŘEHULKOVÁ, H., KOFRONOVA, O., VOHRADSKY, J. Systems insight into the spore germination of Streptomyces coelicolor. Journal of Proteome Research. 2013, 12(44), 525–536. ISSN 1535-3893. IF 5.056 74. ŠALOVSKÁ, B., TICHÝ, A., FABRIK, I., ŘEZÁČOVÁ, M., VÁVROVÁ, J. Comparison of resins for metal oxide affinity chromatography with mass spectrometry detection for the determination of phosphopeptides. Analytical Letters. 2013, 46(10), 1505–1524. ISSN 0003-2719. IF 0.965 75. ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., KORÁBEČNÝ, J., DOLEŽAL, R., ZEMEK, F., BENNION, JB., KUČA, K. Oximes: inhibitors of human recombinant acetylcholinesterase. A structure-activity relationship (SAR) study. International Journal of Molecular Sciences. 2013, 14(8), 16882–16900. ISSN 1422-0067. IF 2.464 76. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., KRÁL, J., HOROVÁ, A., MUSÍLEK, K., SOUKUP, O., DRTINOVÁ, L., GAZOVA, Z., SIPOSOVA, K., KUČA, K. 7-methoxytacrine-adamantylamine heterodimers as cholinesterase inhibitors in Alzheimer’s disease treatment – synthesis, biological evaluation and molecular modeling studies. Molecules. 2013, 18(2), 2397–2418. ISSN 1420-3049. IF 2.428 77. TACHECI, I., KVĚTINA, J., KUNEŠ, M., PAVLÍK, M., KOPÁČOVÁ, M., ČERNÝ, V., REJCHRT, S., VARAYIL, JE., BUREŠ, J. The effect of general anaesthesia on gastric myoelectric activity in experimental pigs. BMC Gastroenterology. 2013, 13(Art. No. 48), 1–6. ISSN 1471- 230X. IF 2.11 78. TAMBOR, V., KACEROVSKÝ, M., LENČO, J., BHAT, G., MENON, R. Proteomics and bioinformatics analysis reveal underlying pathways

22th Annual Report, 2013 – 111 ARTICLES IN JOURNALS WITH IMPACT FACTOR

of infection associated histologic chorioamnionitis in pPROM. Placenta. 2013, 34(2), 155–161. ISSN 0143-4004. IF 3.117 79. VAŠATOVÁ, M., PUDIL, R., HORÁČEK, J., BUCHLER, T. Current applications of cardiac troponin T for the diagnosis of myocardial damage. Advances in Clinical Chemistry. 2013, 2013(61), 33–65. ISSN 0065-2423. IF 3.674 80. VÁVROVÁ, J., ZÁRYBNICKÁ, L., LUKÁŠOVÁ, E., ŘEZÁČOVÁ, M., NOVOTNÁ, E., ŠINKOROVÁ, Z., TICHÝ, A., PEJCHAL, J., ĎURIŠOVÁ, K. Inhibition of ATR kinase with the selective inhibitor VE- 821 results in radiosensitization of cells of promyelocytic leukaemia (HL-60). Radiation and Environmental Biophysics. 2013, 52(4), 471– 479. ISSN 0301-634X. IF 1.754 81. VLČEK, V., POHANKA, M. Toxic products of cyanobacteria. Wulfenia. 2013, 20(9), 197–208. ISSN 1561-882X. IF 0.467 82. VOICU, V., ALBU, F., TACHE, F., MUSÍLEK, K., KUČA, K., MEDVEDOVICI, A. LC-MS/MS approaches for the assay of bis- quaternary pyridinium oximes used as AChE reactivators in biological matrices. Bioanalysis. 2013, 5(7), 793–809. ISSN 1757-6180. IF 3.253 83. ZÁRYBNICKÁ, L., VÁVROVÁ, J., HAVELEK, R., TICHÝ, A., PEJCHAL, J., ŠINKOROVÁ, Z. Lymphocyte subsets and their H2AX phosphorylation in response to in vivo irradiation in rats. International Journal of Radiation Biology. 2013, 89(2), 110–117. ISSN 0955-3002. IF 1.895 84. ZAVADSKA, D., ANCA, I., ANDRÉ, F., BAKIR, M., CHLÍBEK, R., CIZMAN, M., IVASKEVICIENE, I., MANGAROV, A., MÉSZNER, Z., POKORN, M., PRYMULA, R., RICHTER, D., SALMAN, N., SIMURKA, P., TAMM, E., TEŠOVIC, G., URBACIKOVA, I., USONIS, V. Recommendations for tick-borne encephalitis vaccination from the Central European Vaccination Awareness Group (CEVAG). Human Vaccines & Immunotherapeutics. 2013, 9(2), 362–374. ISSN 2164- 5515. 85. ZELENÁ, H., MRÁZEK, J., KUHN, T. Tula hantavirus infection in immunocompromised host, Czech Republic. Emerging Infectious Disease. 2013, 19(11), 1873–1876. ISSN 1080-6040. IF 5.993 86. ZEMEK, F., ŽĎÁROVÁ KARASOVÁ, J., ŠEPSOVÁ, V., KUČA, K. Acetylcholinesterase reactivators (HI-6, obidoxime, trimedoxime, K027, K075, K127, K203, K282): structural evaluation of human serum albumin binding and absorption Kinetics. International Journal of Molecular Sciences. 2013, 14(8), 16076–16086. ISSN 1422-0067. IF 2.464

22th Annual Report, 2013 – 112 ARTICLES IN JOURNAL WITH IMPACT FACTOR

87. ZEMEK, F., KORÁBEČNÝ, J., ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., MUSÍLEK, K., KUČA, K. Albumin and α1-acid glycoprotein: old acquaintances. Expert Opinion on Drug Metabolism & Toxicology. 2013, 9(8), 943–954. ISSN 1742-5255. IF 2.944 88. ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., MUSÍLEK, K., KUČA, K. Time- dependent changes of oxime K027 concentrations in different parts of rat central nervous system. Neurotoxicity Research. 2013, 23(1), 63– 68. ISSN 1029-8428. IF 2.865 89. ŽĎÁROVÁ KARASOVÁ, J., PAVLÍK, M., CHLADEK, J., JUN, D., KUČA, K. Hyaluronidase: Its effects on HI-6 dichloride and dimethanesulphonate pharmacokinetic profile in pigs. Toxicology Letters. 2013, 220(2), 167–171. ISSN 0378-4274. IF 3.145 90. ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., KUNEŠ, M., KVĚTINA, J., CHLÁDEK, J., JUN, D., BUREŠ, J., TACHECÍ, I., KUČA, K. Intravenous application of HI-6 salts (dichloride and dimethansulphonate) in pigs: comparison with pharmacokinetics profile after intramuscular administration. Neuroendocrinology Letters. 2013, 34(suppl. 2), 74–78. ISSN 0172-780X. IF 0.932 91. ŽĎÁROVÁ KARASOVÁ, J., CHLÁDEK, J., HROCH, M., FUSEK, J., HNÍDKOVÁ, D., KUČA, K. Pharmacokinetics study of two acetylcholinesterase reactivators, trimedoxime and newly synthesized oxime K027, in rat plasma. Journal of Applied Toxicology. 2013, 33(1), 18–23. ISSN 0260-437X. IF 2.597

22th Annual Report, 2013 – 113 ARTICLES IN OTHERS JOURNALS ARTICLES IN OTHERS JOURNALS

1. BAJGAR, J. Laboratory examination in nerve agent intoxication. Acta Medica (Hradec Králové). 2013, 56(3), 89–96. ISSN 1211-4286.

2. BAREKOVÁ, L., ZÁLABSKÁ, E., HANOVCOVÁ, I. Experience with laboratory diagnosis of Clostridium difficile. Klinická mikrobiologie a infekční lékařství. 2013, 19(4), 86–90. ISSN 1211-264X. 3. BOŠTÍK, P. Lyme disease. i-med. 2013, 3(August), Art. No. P1649. ISSN 1338-4392. 4. BOŠTÍK, P. HIV infection. i-med. 2013, 3(January), Art. No. P1385. ISSN 1338-4392. 5. BOŠTÍKOVÁ, V., BOŠTÍK, P. Rotavirus infections. i-med. 2013, 3(February), Art. No. P1436. ISSN 1338-4392.

6. BOŠTÍKOVÁ, V., BOŠTÍK, P. 10th Annual International Consequence Managment Seminar, Tel Aviv, Izrael. Listy Univerzity obrany. 2013, (2), 11. 7. BOŠTÍKOVÁ, V., PRÁŠIL, P., SLEHA, R., KAISLEROVÁ, L., COUFALOVÁ, M., MOSIO, P., BOŠTÍK, P. Viral hemorrhagic fevers. Vakcinologie. 2013, 7(4), 173–185. ISSN 1802-3150.

8. BOŠTÍKOVÁ, V. Molecular epidemiology of VZV. i-med. 2013, 3(February), Art. No. 1336. ISSN 1338-4392. 9. BOŠTÍKOVÁ, V. B-agents and bioterrorism issues. i-med. 2013, 3(February), Art. No. P1366. ISSN 1338-4392.

10. BOŠTÍKOVÁ, V., SALAVEC, M., SMETANA, J., CHLÍBEK, R., KAISLEROVÁ, L., PRÁŠIL, P., SLEHA, R., BOŠTÍK, P. Herpetic infections with emphasis on manifestations in children. Pediatrie pro praxi. 2013, 2013(1), 8–12. ISSN 1213-0494.

11. BOŠTÍKOVÁ, V., SALAVEC, M. Infection with human papillomavirus. i- med. 2013, 3(July), Art. No. P1419. ISSN 1338-4392.

12. BOŠTÍKOVÁ, V. Viral hemorrhagic fever – question the near future? i- med. 2013, 3(January), Art. No. P1337. ISSN 1338-4392.

13. BOŠTÍKOVÁ, V. Travel medicine – it is only a vaccination against exotic diseases. i-med. 2013, 3(February), Art. No. P1360. ISSN 1338-4392.

14. BOŠTÍKOVÁ, V., SLEHA, R., SALAVEC, M., MOSIO, P., KUSÁK, R., MAZUROVÁ, I. The problem of bacterial infection, one of the possible

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causes of infertility in humans. i-med. 2013, 3(March), Art. No. P1418. ISSN 1338-4392. 15. BOŠTÍKOVÁ, V. News in vaccinology. Vakcinologie. 2013, 7(4), 195– 196. ISSN 1802-3150. 16. BOŠTÍKOVÁ, V., ŠPLIŇO, M. Poliomyelitis – polioeradikační strategy. i- med. 2013, 3(March), Art. No. P1425. ISSN 1338-4392.

17. BOŠTÍKOVÁ, V., SALAVEC, M. Sexually transmitted diseases – syphilis and gonorrhea. i-med. 2013, 3(March), Art. No. P1437. ISSN 1338- 4392. 18. BOŠTÍKOVÁ, V., SLEHA, R., KAISLEROVÁ, L. News in vaccinology. Vakcinologie. 2013, 7(1), 41–43. ISSN 1802-3150.

19. BOŠTÍKOVÁ, V., SALAVEC, M., SMETANA, J., CHLÍBEK, R., KAISLEROVÁ, L., PRÁŠIL, P., SLEHA, R., BOŠTÍK, P. Human herpesviruses. i-med. 2013, 2013(February), Art. No. P1320. ISSN 1338-4392. 20. BOŠTÍKOVÁ, V., SLEHA, R., KAISLEROVÁ, L. News in vaccinology. Vakcinologie. 2013, 7(2), 85–89. ISSN 1802-3150.

21. BOŠTÍKOVÁ, V., SLEHA, R., KAISLEROVÁ, L. News in vaccionology. Vakcinologie. 2013, 7(3), 139–141. ISSN 1802-3150.

22. BOŠTÍKOVÁ, V., SALAVEC, M., SLEHA, R., PRÁŠIL, P., KAISLEROVÁ, L., BOŠTÍK, P. Viral illness with blistering, diagnostics and therapy. Referátový výběr z dermatovenerologie. 2013, 55(1), 58– 64. ISSN 1213-9106. 23. ČEČKA, F., JON, B., ŠUBRT, Z., FERKO, A. Pancreatic fistula – definition, risk factors and treatment options. Rozhledy v chirurgii. 2013, 92(2), 77–84. ISSN 0035-9351.

24. DOHNAL, V., DOHNALOVÁ, L., JUN, D., KUČA, K. Selective monitoring of enzymatic activity of acetylcholinesterase by flow injection analysis with mass spectrometric detection. Military Medical Science Letters. 2013, 82(3), 120–125. ISSN 0372-7025.

25. DOLÉNKOVÁ, T., HLÚBIK, P. Eating habits of adolescents and adults as a colorectal cancer prevention. Ošetřovatelství a porodní asistence. 2013, 4(4), 670–677. ISSN 1804-2740.

26. DRTINOVÁ, L., POHANKA, M. Antioxidant markers in guinea pig exposed to Obidoxime and HI-6 acetylcholinesterase oxime reactivators containing oxime moiety. African Journal of Pharmacy and Pharmacology. 2013, 7(31), 2252–2257. ISSN 1996-0816.

22th Annual Report, 2013 – 115 ARTICLES IN OTHERS JOURNALS

27. FAJFROVÁ, J. Non-alcoholic fatty liver disease (NAFLD) as a part of metabolic syndrom. i-med. 2013, 3(October), Art. No.1656. ISSN 1338-4392. 28. FAJFROVÁ, J., PAVLÍK, V. Vitamins, its function and use. Medicína pro praxi. 2013, 10(2), 81–84. ISSN 1214-8687.

29. FERKÁL, P., RŮŽIČKA, M. ACR medical support in ISAF operations. Zpravodaj vojenské farmacie. 2013, 23(3), 11–19. ISSN 1213-8029.

30. HALAJČUK, T., PROCHÁZKA, M., JEŽEK, B., VANĚK, J. Selected cost indicators of pre-hospital emergency care in the Czech Republic. Journal of International Scientific Publication: Economy and Business. 2013, 7(2), 361–370. ISSN 1313-2555.

31. HLUBIK, J., HLÚBIK, P., LHOTSKÁ, L. Changes in bioimpedance depending on conditions. Hygiena Public Health. 2013, 48(2), 185–188. ISSN 1509-1945. 32. HLÚBIK, J., LHOTSKÁ, L., HLÚBIK, P., STŘÍTECKÁ, H. Changes in bioimpedance and body composition parameters depending on measurement conditions. Hygiena Public Health. 2013, 48(2), 181– 184. ISSN 1509-1945. 33. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Evaluation of serum levels of multiple cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia using biochip array technology. Experimental Oncology. 2013, 35(3), 229–230. ISSN 0204-3564. 34. HUMLÍČEK, V. Czech Republic. Almanac of Medical Corps Worldwide. Supplement of Medical Corps International Forum. 2013, (February), 39–43. 35. CHLÍBEK, R., SMETANA, J., BOŠTÍKOVÁ, V., ŠPLIŇO, M., HANOVCOVÁ, I. Vaccination schedules in Czech Republic vs. the European Union. Vakcinologie. 2013, 7(3), 108–114. ISSN 1802-3150.

36. CHLÍBEK, R. Myths and misconceptions in vaccination. První linie. 2013, 3(2), 23–27. ISSN 1804-9028.

37. CHLÍBEK, R. The risk group vaccination against pneumococcal diseases. Medicína po promoci. 2013, 14(1), 44–48. ISSN 1212-9445.

38. CHLÍBEK, R. (Editor). Proceedings of Abstracts – 8th Hradec Immunisation Days. Vakcinologie. 2013, 7(1), 20–38. ISSN 1802-3150.

39. KLEIN, L. The MBC session at the 15th EBA congress in Vienna. Annals of Burns and Fire Disasters. 2013, 26(3), 162–163. ISSN 1592-9566.

22th Annual Report, 2013 – 116 ARTICLES IN OTHER JOURNALS

40. KLEIN, L., GUNN, W., BOHONĚK, M., KLEIN, T. Military humanitarian assistance to civilian populations in times of conflict – the Czech experience. Journal of Defense Management. 2013, 2013(S3), 1–4. ISSN 2167-0374. 41. KLEIN, L. Important step ahead in mutual co-operation between MBC and EBA. Annals of Burns and Fire Disasters. 2013, 26(3), 163. ISSN 1592-9566. 42. KLEIN, L. Workshop in Hradec Králové, Czech Republic, on states of preparedness for mass-casualty situations. Military Medical Science Letters. 2013, 82(2), 87–91. ISSN 0372-7025.

43. KLOSOVÁ, H., KLEIN, L. Surgical procedures in treatment of burns injuries. Rozhledy v chirurgii. 2013, 92(5), 269–272. ISSN 0035-9351. 44. KONŠTACKÝ, S. Care for generations. Bulletin Sdružení praktických lékařů ČR. 2013, 23(5), 3–5. ISSN 1212-6152.

45. KONŠTACKÝ, S. Was the program on smoking cessation a benefit to the Army? Medical Tribune. 2013, 9(8), B5–B5. ISSN 1214-8911. 46. KONŠTACKÝ, S. Do we know how to treat cough? Bulletin Sdružení praktických lékařů ČR. 2013, 23(1), 27–28. ISSN 1212-6152.

47. KONŠTACKÝ, S. Do you believe the National program of a complex medical care is the right direction? Zdravotnické noviny - Lékařské listy. 2013, 62(12), 5. ISSN 0044-1996.

48. KONŠTACKÝ, S. Respiratory illnesses in Genenral Pracititioner's clinic. Acta medicinae. 2013, 2(9), 74–76. ISSN 1805-398X.

49. KOSINA, P., RUMLAROVÁ, Š., PLÍŠEK, S., SMETANA, J. Clinical manifestations of pneumococcal infections and the current prevention options. Klinická mikrobiologie a infekční lékařství. 2013, 19(4), 120– 127. ISSN 1211-264X. 50. KRAČMAROVÁ, A., BARTOŠOVÁ, L., ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., BAJGAR, J. Transdermal penetration of the acetyl- cholinesterase reactivator HI-6 in a rat model. Military Medical Science Letters. 2013, 82(4), 185–188. ISSN 0372-7025.

51. KRAUSOVÁ, J., KOSINA, P., SMETANA, J. Vaccination in adults – general principles. Medicína pro praxi. 2013, 10(4), 142–145. ISSN 1214-8687. 52. KUČA, K., MUSÍLEK, K., KORÁBEČNÝ, J., ŽĎÁROVÁ KARASOVÁ, J., SOUKUP, O., HRABINOVÁ, M., JUN, D. Influence of the distance between quaternary nitrogen and oxime group on the reactivation ability

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of oximes – antidotes against nerve agents. Journal of Environmental Immunology and Toxicology. 2013, 1(1), 41–43. ISSN 2225-1219.

53. LORKE, DE., NURULAIN, SM., HASAN, MY., KUČA, K., PETROIANU, GA. Five experimental bispyridinium oximes in comparison with the conventional oximes pralidoxime and obidoxime: in vivo efficacy to protect from azinphos-methyl-induced toxicity. Journal of Environmental Immunology and Toxicology. 2013, 1(1), 44–50. ISSN 2225-1219. 54. MISÍK, J. Military incapacitating agent BZ (3-quinuclidinyl benzilate) – past, present and future. Military Medical Science Letters. 2013, 2013(82), 115–119. ISSN 0372-7025.

55. PATOČKA, J., SOUKUP, O., KUČA, K. Ladostigil – another pursuit of a dual action cognitive enhancer. Psychiatrie. 2013, 17(2), 13–15. ISSN 1211-7579. 56. PAVLÍK, V., FAJFROVÁ, J. The hygienic conditions in Afghanistan. Medical Corps International Forum. 2013, 12(1), 48–49.

57. PAVLÍK, V. Glance back at the national conference Nutrition and health. Military Medical Science Letters. 2013, 82(3), 133–134. ISSN 0372- 7025. 58. PAVLIŠ, O., POHANKA, M. Dividing of cholinesterase inhibitors. Zpravodaj vojenské farmacie. 2013, 23(1), 32–36. ISSN 1213-8029.

59. POHANKA, M., PAVLIŠ, O. Melatonin Changes tularemia progression in a BALB/c mouse model. African Journal of Pharmacy and Pharmacology. 2013, 7(27), 1917–1923. ISSN 1996-0816.

60. POHANKA, M. Butyrylcholinesterase and its using in diagnosis. Zpravodaj vojenské farmacie. 2013, 23(2), 15–18. ISSN 1213-8029.

61. POHNÁN, R., ROZWADOWSKI, F., KLEIN, L., RYSKA, M. Advantages and disadvantages of transabdominal preperitoneal approach and total extraperitoneal approach versus open repair of inguinal hernia. Military Medical Science Letters. 2013, 82(1), 25–31. ISSN 0372-7025.

62. POLÁK, P., KOSINA, P., BLECHOVÁ, Z., KOTEN, J., ROZSYPAL, H., CHLÍBEK, R., BENEŠ, J. Guidelines for treatment of patients with impaired or lost splenic function (hyposplenism/asplenia). Vakcinologie. 2013, 7(3), 102–107. ISSN 1802-3150.

63. POTUŽNÍKOVÁ, D., HELMUT, T., JUNEK, P., BEDNARČÍK, P., FIALA, Z. Legislative intent of the noise act with respect to traffic noise control. Transactions on Transport Sciences. 2013, 6(2), 81–88. ISSN 1802- 971X.

22th Annual Report, 2013 – 118 ARTICLES IN OTHER JOURNALS

64. PROCHÁZKA, M., HALAJČUK, T., JEŽEK, B., VANĚK, J., MAŠEK, J. Traumatology planning in the Czech Republic in the context of new legislation I. Urgentní medicína. 2013, 16(2), 6–11. ISSN 1212-1924.

65. PROCHÁZKA, M., HALAJČUK, T., MAŠEK, J., JEŽEK, B., VANĚK, J. Financing of the pre-hospital emergency care in the Czech Republic. Journal of International Scientific Publication: Economy and Business. 2013, 7(2), 158–166. ISSN 1313-2555.

66. PRYMULA, R. PCVS versus PPVS: new guidelines and recommendations. Vakcinologie. 2013, 7(1), 32–32. ISSN 1802- 3150. 67. RADOCHA, J., MAISNAR, V., ZAVŘELOVÁ, A., CERMANOVÁ, M., LÁNSKÁ, M., KMONÍČEK, M., JEBAVÝ, L., BLÁHA, M., MALÝ, J., ŽÁK, P. Ffteen years of single center experience with stem cell transplantation for multiple myeloma: a retrospective analysis. Acta Medica (Hradec Králové). 2013, 56(1), 9–13. ISSN 1211-4286.

68. RŮŽIČKA, M., STŘÍTECKÁ, H., WITT, P., HLÚBIK, P. Actual state of affairs of the training of the medical. Military Pharmacy and Medicine. 2013, 6(1), 39–44. ISSN 1898-6498.

69. SALAVEC, M., BOSTIK, V., PRASIL, P., SMETANA, J., ŠPLIŇO, M., CHLÍBEK, R., LOUDA, M., BOŠTÍK, P. Opposite trends of incidence of gonorrhea and other STIs in the eastern Bohemia during past 15 years. Military Medical Science Letters. 2013, 2013(4), 156–161. ISSN 0372-7025. 70. SLANINKA, I., KLEIN, L., HOŠEK, F., HASENÖHRLOVÁ, L., JÍŠKA, S., KAŠKA, M. The use of plasma rich in thrombocytes in the wound healing – some new information and the first experience. Hojení ran. 2013, 7(2), 25–27. ISSN 1802-6400.

71. SLEHA, R., BOŠTÍKOVÁ, V., SALAVEC, M., BOŠTÍK, P., KUSAKOVA, E., KUKLA, R., MOSIO, P., VYDRZALOVA, M., MAZUROVA, J. Mycoplasma infections in humans. Military Medical Science Letters. 2013, 82(4), 142–148. ISSN 0372-7025.

72. SMETANA, J., KOSINA, P., BOŠTÍKOVÁ, V., CHLÍBEK, R. Vaccination of adults with COPD and asthma. Vakcinologie. 2013, 7(4), 169–172. ISSN 1802-3150. 73. SMETANA, J., KOSINA, P., BOŠTÍKOVÁ, V., HANOVCOVÁ, I., ŠPLIŇO, M., CHLÍBEK, R. Vaccination in high-risk groups of patients in the GP's consulting room for adults. Medicína pro praxi. 2013, 10(5), 178–182. ISSN 1214-8687.

22th Annual Report, 2013 – 119 ARTICLES IN OTHERS JOURNALS

74. STŘÍTECKÁ, H. Food safety and quality of foodstuff. Bio&Life. 2013, 1(11–12), 72–73. ISSN 1338-9890.

75. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Ebola virus aerosol transmission from pig to primate. Vakcinologie. 2013, 7(2), 83–84. ISSN 1802-3150.

76. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Humann avian influenza A H7N9 – China. Vakcinologie. 2013, 7(3), 135–138. ISSN 1802-3150.

77. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Influenza – current circulation swine viruses in the world. Vakcinologie. 2013, 7(1), 39–40. ISSN 1802-3150.

78. ŠPLIŇO, M., BOŠTÍKOVÁ, V. SEOUL hantavirus in UK – England and Wales. Vakcinologie. 2013, 7(4), 192–194. ISSN 1802-3150.

79. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B., ÖRHALMI, J. Classification of surgical complications: analysis of the group of consecutive patients. Rozhledy v chirurgii. 2013, 91(12), 666–669. ISSN 0035-9351. 80. VLČEK, V., POHANKA, M. Neurotoxic pesticides and their influence on environment. Phytopedon. 2013, 12(1), 43–47. ISSN 1336-1120.

81. ŽĎÁROVÁ KARASOVÁ, J., KORÁBEČNÝ, J., ZEMEK, F., ŠEPSOVÁ, V., KUČA, K. Acetylcholinesterase inhibitors used or tested in Alzheimer’s disease therapy; their passive diffusion through blood brain barrier: in vitro study. African Journal of Pharmacy and Pharmacology. 2013, 7(22), 1471–1481. ISSN 1996-0816.

82. ŽĎÁROVÁ KARASOVÁ, J., JUN, D., KUČA, K. Screening of blood-brain barrier penetration using the immobilized artificial membrane phosphatidylcholine column chromatography at the physiological pH. Military Medical Science Letters. 2013, 82(2), 55–62. ISSN 0372-7025.

22th Annual Report, 2013 – 120 TEXTBOOKS, MONOGRAPHS, ARTICLES IN MONOGRAPHS AND PROCEEDINGS

TEXTBOOKS, MONOGRAPHS, ARTICLES IN MONOGRAPHS AND PROCEEDINGS

1. BAJGAR, J. Chemical warfare agents used as the content of chemical weapons. In Biologické a chemické zbrane - pripravenosť a odpoveď. Banská Bystrica: PRO, 2013, p. 539–599. ISBN 978-80-89057-43-6 2. BAJGAR, J. Prophylactic possibilities in case of high risk of exposure to nerve agents. In Disaster management. Medical prepardness, response and homeland security. ARORA, R. (Ed.) ; ARORA, P. (Ed.) Boston: CABI Publishing, 2013, p. 425–445. ISBN 978-1-84593-929-8 3. FINTORA, J., VANĚK, J., JEŽEK, B. Crowd Rendering Based on Makehuman™ Data. In Sborník příspěvků 33. konference o geometrii a grafice. Ostrava-Poruba: Vysoká škola báňská – Technická univerzita Ostrava, 2013, vol. 33, p. 83–96. ISBN 978-80-248-3251-7 4. HALAJČUK, T., PROCHÁZKA, M. Economy framework ot the emergency medical service in the Czech Republic. Sborník recenzovaných příspěvků z mezinárodní konference Hradecké ekonomické dny 2013, díl I. Hradec Králové: Gaudeamus, Univerzita Hradec Králové, 2013, p. 175–181. ISBN 978-80-7435-249-2

5. HALAJČUK, T., PROCHÁZKA, M., JEŽEK, B., VANĚK, J., ANTOŠ, K. The last year in Disaster Medicine, what happend since last DM conference. In Sborník příspěvků 10. ročníku celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. Hradec Králové: ZSA, 2013, p. 31–33. ISBN 978-80-905089-2-7 6. CHLÍBEK, R. Aluminum compounds. In Očkovanie v špeciálnych situáciách. Praha: Mladá fronta, 2013, p. 205–216. ISBN 978-80-204- 2805-9 7. CHLÍBEK, R. Mercury compounds. In Očkovanie v špeciálnych situáciách. Praha: Mladá fronta, 2013, p. 216–223. ISBN 978-80-204- 2805-9

8. CHLÍBEK, R. Vaccination of persons with chronic renal failure and in those on hemodialysis. In Očkovanie v špeciálnych situáciách. Praha: Mladá fronta, 2013, p. 153–160. ISBN 978-80-204-2805-9 9. JANEČKA, P., PETRÁNEK, K., JEŽEK, B., VANĚK, J. Implicit surface visualization using Raymarching. In Sborník příspěvků 33. konference o geometrii a grafice. Ostrava-Poruba: Vysoká škola báňská – Technická univerzita Ostrava, 2013, vol. 33, p. 133–124. ISBN 978-80- 248-3251-7

22th Annual Report, 2013 – 121 TEXTBOOKS, MONOGRAPHS, ARTICLES IN MONOGRAPHS AND PROCEEDINGS

10. JEŽEK, B., VANĚK, J. Heuristic label placement in image. In Sborník příspěvků 33. konference o geometrii a grafice. Ostrava-Poruba: Vysoká škola báňská – Technická univerzita Ostrava, 2013, vol. 33, p. 125–130. ISBN 978-80-248-3251-7 11. KAREN, I., PRYMULA, R., CHLÍBEK, R., MANĎÁKOVÁ, Z., ĎURČOVIČ, J. Vaccination in the GP's consulting room, prophylaxis of malaria and traveler's diarrhea. In Všeobecné praktické lékařství. Praha: Galén, 2013, p. 571–599. ISBN 978-80-7262-934-3 12. KLEMENT, C., MEZENCEV, R., BAJGAR, J., MAĎAROVÁ, L., OLEÁR, V. Selected terminology of biological and chemical weapons. In Biologické a chemické zbrane – pripravenosť a odpoveď. Banská Bystrica: PRO, 2013, p. 601–673. ISBN 978-80-89057-43-6 13. KLEMENT, C., MEZENCEV, R., BAJGAR, J. Biological and chemical weapons – prepardness and response. 1. ed. Banská Bystrica: PRO, 2013. 73 p. ISBN 978-80-89057-43-6 14. KUBELKOVÁ, K., KROČOVÁ, Z., PLZÁKOVÁ, L., MACELA, A. The role of B cells in intracellular bacterial pathogen infections. In B Cells: Molecular Biology, Developmental Origin and Impact on the Immune System. MONTES, RL. (Ed.) ; REYES, L. (Ed.) New York: Nova Science Publishers – NY, 2013, p. 1–44. ISBN 978-1-62808-541-9 15. MRVEČKA, P., HUMLÍČEK, V. The field exercise Elbe 2013. Hradec Králové: Univerzita obrany – Fakulta vojenského zdravotnictví, 2013. 9 p. 16. PAVLÍK, V., HLÚBIK, P., FAJFROVÁ, J. Current preventive program in the ACR. In Sborník vědeckých prací konference Životné podmienky a zdravie. Banská Bystrica: Slovenská epidemiologická asociácia, 2013, p. 125-127. ISBN 978-80-7159-215-0 17. PEJCHAL, J., ŠINKOROVÁ, Z., TICHÝ, A., VÁVROVÁ, J., VILASOVÁ, Z., ZÁRYBNICKÁ, L. Biophysics for rescuers (Part 1). Vol. 369, Hradec Králové: Univerzita obrany – Fakulta vojenského zdravotnictví, 2013. 86 p.

18. PETRÁNEK, K., VANĚK, J., JEŽEK, B., JANEČKA, P. Robust Image Comparison Using Mutual Information. In Sborník příspěvků 33. konference o geometrii a grafice. Ostrava-Poruba: Vysoká škola báňská – Technická univerzita Ostrava, 2013, vol. 33, p. 199–204. ISBN 978-80- 248-3251-7 19. POHANKA, M. Overview of toxicology. Studijní texty Fakulty vojenského zdravotnictví Univerzity obrany v Hradci Králové. Sv. 370, Brno: Univerzita obrany, 2013. 54 p.

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20. PROCHÁZKA, M. (Ed), JEŽEK, B. (Ed), VANĚK, J. (Ed), ANTOŠ, K. (Ed) Proceedings of the 10th Conference on Disaster Medicine – Experience, Preparation, Practice. Sborník příspěvků 10. ročníku celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. Hradec Králové: ZSA, 2013. 92 p. ISBN 978-80-905089-2-7 21. PRYMULA, R., CHLÍBEK, R. Vaccination of pregnant and lactating women. In Očkovanie v špeciálnych situáciách. Praha: Mladá fronta, 2013, p. 160–168. ISBN 978-80-204-2805-9 22. PSUTKA, J., MALÝ, V., HUMLÍČEK, V., MRVEČKA, P. MEDEVAC 2011–2013. In Sborník příspěvků 10. ročníku celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. Hradec Králové: ZSA, 2013, vol. 10, p. 65–66. ISBN 978–80-905089-2-7 23. VANĚK, J., JEŽEK, B. Computer graphics subjects in computer science curricula. In Proceedings of the 10th International Conference Efficiency and Responsibility in Education 2013. Trnava: Česká zemědělská univerzita, 2013, vol. 10, p. 626–633. ISBN 978-80-213-2378-0

22th Annual Report, 2013 – 123 ABSTRACTS IN JOURNAL WITH IMPACT FACTOR ABSTRACTS IN JOURNALS WITH IMPACT FACTOR

1. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., JEBAVÝ, L., JAKL, M., ŽÁK, P. Serum levels of multiple cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia and in healthy subjects. Haematologica-The Hematology Journal. 2013, 2013(98), 500. ISSN 0390-6078. IF 5.935

2. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Evaluation of serum cytokine and adhesion molecule profile in newly diagnosed acute leukemias using biochip array technology. Blood. 2013, 122(21), 4975. ISSN 0006-4971. IF 9.06

3. JAKL, M., MALÝ, J., FÁTOROVÁ, I., HORÁČEK, J., PUDIL, R. High on- treatment platelet reactivity: not an isolated disorder. Haematologica-The Hematology Journal. 2013, 98(suppl. 1), 208. ISSN 0390-6078. IF 5.935

4. KUPSA, T., HORÁČEK, J., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Serum profile of multiple cytokines and adhesion molecules in newly diagnosed AML patiens differs in those who did not reach complete remission after Antrhracyclin/Cytarabine induction therapy. Blood. 2013, 122(21), 4997. ISSN 0006-4971. IF 9.06

5. KUPSA, T., HORÁČEK, J., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Evaluation of serum levels of multiple cytokines and adhesion molecules in patients treated for acute myeloid leukemia. Haematologica-The Hematology Journal. 2013, 98(suppl. 1), 763. ISSN 0390-6078. IF 5.935

6. MISÍK, J., KRAČMAROVÁ, A., KASSA, J. Effect of cholinesterase inhibitors on cholinergic deficit induced by 3-quinuclidinyl benzilate in rats performing the multiple t-maze. European Neuropsycho- pharmacology. 2013, 23(2), 537–538. ISSN 0924-977X. IF 4.595

7. OSIČKOVÁ, J., KOVÁČOVÁ, V., PIKULA, J., BANĎOUCHOVÁ, H., KRÁL, J., SKOCHOVÁ, H., POHANKA, M., NOVOTNÝ, L. Oxidative stress in birds: Effects of heavy metals and pharmaceuticals. Toxicology Letters. 2013, 221(S), S111–S111. ISSN 0378-4274. IF 3.145

8. STŘÍTECKÁ, H., HLÚBIK, P. Effects to satiation and weight reduction of fermented milk product enriched with protein and fibre. Annals of Nutrition and Metabolism. 2013, 63(supl.1), 436. ISSN 0250-6807. IF 1.661

9. STŘÍTECKÁ, H., ZMRZLÁ, H., HLÚBIK, P. Is there any differences? – Comparison of eating habits at school children with normal and excess

22th Annual Report, 2013 – 124 ABSTRACTS IN JOURNAL WITH IMPACT FACTOR

weight/obesity. Annals of Nutrition and Metabolism. 2013, 63(supl.1), 721. ISSN 0250-6807. IF 1.661

10. STŘÍTECKÁ, H., HLÚBIK, P. How the Czech Army solves the problem with obesity. Obesity Facts (print). 2013, 6(suppl. 1), 188. ISSN 1662- 4025. IF 1.583

11. STŘÍTECKÁ, H., HLÚBIK, P., ZMRZLÁ, H. The eating habits of primary school children – comparison of children wit normal and excess weight/ obesity. Obesity Facts (print). 2013, 6(suppl. 1), 221–222. ISSN 1662- 4025. IF 1.583

12. ŠEPSOVÁ, V., KRŮŠEK, J., DRTINOVÁ, L., POHANKA, M., ŽĎÁROVÁ KARASOVÁ, J. Myasthenia gravis: interaction of new acetyl- cholinesterase inhibitors with the nicotinic receptors. European Journal of Neurology. 2013, 19(SI), 380. ISSN 1351-5101. IF 4.162

22th Annual Report, 2013 – 125 ABSTRACTS ABSTRACTS

1. BOŠTÍKOVÁ, V., BOŠTÍK, P., PRÁŠIL, P., SALAVEC, M., SMETANA, J., CHLÍBEK, R., KOSINA, P., PLÍŠEK, S. Genetic diversity of Varicella zoster virus (VZV) in Europe focusing on Czech Republic – what´s now, and what´s next. In: 8th WSPID 2013 Congress. Geneva: Kenes International. 2013, p. 1.

2. ČÁP, R., SLANINKA, I., KLEIN, L. Mutilating injuries of six-year boy. Hojení ran. 2013, 7(2), 8. ISSN 1802-6400.

3. FERKO, A., ŠUBRT, Z., ČEČKA, F., JON, B. Laparoscopic vs. open liver resection for metastatic colorectal cancer: oncological results. In: 10. Ostravské dny miniinvazivní chirurgie. Ústí nad Labem: BOS. 2013, p. 9. ISBN 978-80-87562-13-0.

4. HORÁČEK, J., VAŠATOVÁ, M., PUDIL, R., JAKL, M., ŽÁK, P., JEBAVÝ, L. Multiple biomarkers in the detection of cardiotoxicity during stem cell transplantation for hematological malignancies. In: Proceedings and Abstract Book, 4th International Congress on Leukemia Lymphoma Myeloma. Ankara: 2013, p. 358–359.

5. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Serum concentrations of cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia. In: 27. Olomoucké hematologické dny s mezinárodní účastí – sborník abstrakt. Vydavatelství UP Olomouc. 2013, p. 58-59. ISBN 978-80-244-3480-3.

6. HORÁČEK, J., JEBAVÝ, L., VAŠATOVÁ, M., TICHÝ, M., ŽÁK, P. Assessment of cardiotoxicity induced by conventional and high-dose chemotherapy for acute leukemia with multiple biomarkers of cardiac injury. Military Medical Science Letters. 2013, 82(suppl. 1), 9. ISSN 0372-7025.

7. HORÁČEK, J., VAŠATOVÁ, M., PUDIL, R., ŽÁK, P., JEBAVÝ, L. The use of cardiac biomarkers in the detection of cardiotoxicity associated with stem cell transplantation for hematological malignancies. Military Medical Science Letters. 2013, 82(suppl. 1), 9. ISSN 0372-7025.

8. HORÁČEK, J., JEBAVÝ, L., VAŠATOVÁ, M., ŽÁK, P., TICHÝ, M., MALÝ, J. Multiple biomarkers in the detection of cardiotoxicity induced by conventional and high-dose chemotherapy for acute leukemia. In: Proceedings and Abstract Book, 4th International Congress on Leukemia Lymphoma Myeloma. Ankara: 2013, p. 354.

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9. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., ŽÁK, P., JEBAVÝ, L. Serum cytokine and adhesion molecule levels in patients with acute lymphoblastic leukemia and in healthy subjects. In: Proceedings and Abstract Book, 4th International Congress on Leukemia Lymphoma Myeloma. Ankara: 2013, p. 282.

10. HORÁČEK, J., CERMANOVÁ, M., ZAVŘELOVÁ, A., LÁNSKÁ, M., JEBAVÝ, L., ŽÁK, P. Treatment of adult acute lymphoblastic leukemia according to GMALL 07/2003 protocol – single center experience. In: 27. Olomoucké hematologické dny s mezinárodní účastí – sborník abstrakt. Olomouc: Vydavatelství UP Olomouc. 2013, p. 24. ISBN 978-80-244- 3480-3.

11. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., ŽÁK, P., JEBAVÝ, L. Evaluation of serum cytokine and adhesion molecule concentrations in patients treated for acute myeloid leukemia. In: 27. Olomoucké hematologické dny s mezinárodní účastí – sborník abstrakt. Brno: Masarykův onkologický ústav. 2013, p. 110. ISBN 978-80-904596-9-4.

12. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., ŽÁK, P., JEBAVÝ, L. Evaluation of serum levels of adhesion molecules by biochip array technology in acute myeloid leukemia patients. In: Biochimica Clinica. Milano: BIOMEDIA. 2013, p. 200.

13. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., JEBAVÝ, L., ŽÁK, P. Serum levels of multiple cytokines and adhesion molecules in patients treated for acute myeloid leukemia. In: Proceedings and Abstract Book, 4th International Congress on Leukemia Lymphoma Myeloma. Ankara: 2013, p. 315–316.

14. HRABINOVÁ, M., CAHLÍKOVÁ, L., CHLEBEK, J., BENEŠOVÁ, N., KUČA, K., OPLETATL, L., JUN, D. Novel prolyl oligopeptidase inhibitors from the group of isoquinoline alkaloids – possible candidates for Alzheimer disease drug discovery. Military Medical Science Letters. 2013, 82(suppl. 1), 9–10. ISSN 0372-7025.

15. CHLÍBEK, R. News in a multi approach to vaccination of adults. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 72–72. ISBN 978-80-260-4939-5.

16. CHLÍBEK, R. Current vaccination schedules of European countries in comparison with the Czech calendar. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 41–42. ISBN 978-80-260-4939-5.

17. CHLÍBEK, R. The procedure for checks protection and possible vaccination against HBV. Vakcinologie. 2013, 7(1), 24–25. ISSN 1802- 3150.

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18. CHLÍBEK, R., MACHAČ, J., SMETANA, J., FABIÁNOVÁ, K., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., NÁPLAVA, P., LŽIČAŘOVÁ, D. The results of seroprevalence studies of pertussis in the Czech Republic – influence vaccination strategy. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 66–66. ISBN 978-80-260-4939-5.

19. CHLÍBEK, R., SMETANA, J., FABIÁNOVÁ, K., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., LŽÍČAŘOVÁ, D. Seroprevalence of Bordetella pertussis in adults in the Czech Republic. In: Český kongres o infekčních nemocech – sborník abstrakt. Brno: Společnost infekčního lékařství. 2013, p. 17–17. ISBN 978-80-260-4768-1.

20. CHLÍBEK, R. Priorities for vaccination in 2013 from the perspective of GPs. Medicína pro praxi. 2013, 10(8), 10–10. ISSN 1214-8687.

21. CHLÍBEK, R. News vaccination in adults. Vakcinologie. 2013, 7(1), 24– 24. ISSN 1802-3150.

22. CHLÍBEK, R. Trends in adult vaccination and the introduction of new vaccines. Pediatria / Pediatrics. 2013, 8(1), 15–16. ISSN 1336-863X.

23. CHLÍBEK, R. Multiconsensunal view in vaccination. In: Wonca 2013. Prague: Wonca. 2013, p. 23.

24. CHLÍBEK, R. Non-standard situations in vaccination practice GP`s. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 81–81. ISBN 978-80-260-4939-5.

25. JUN, D., KŘENKOVÁ, Z., MUSÍLEK, K., KUČA, K. Bioscavengers – efficient antidotes for poisoning by nerve agents and organophosphorus pesticides. In: Military Medical Science Letters. Hradec Králové: University of Defence, Faculty of Military Health Sciences. 2013, p. 14.

26. KRAČMAROVÁ, A., ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., BAJGAR, J. In vitro testing of HI-6 skin permeation. Military Medical Science Letters. 2013, 82(suppl. 1), 17–17. ISSN 0372-7025.

27. KUPSA, T., HORÁČEK, J., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Evaluation of serum concentrations of multiple cytokines and adhesion molecules in patients with newly diagnosed acute myeloid leukemia. In: 27. Olomoucké hematologické dny s mezinárodní účastí – sborník abstrakt. Olomouc: UP Olomouc. 2013, p. 58. ISBN 978-80-244-3480-3.

28. KUPSA, T., HORÁČEK, J. Evaluation of serum levels of multiple cytokines and adhesion molecules in patients treated for acute myeloid leukemia. In: Abstracts book of the 10th International Medical Postgraduate Conference. 2013, p. 49–51. ISBN 978-80-260-5300-2.

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29. LOCHMAN, P., LEŠKO, M., PÁRAL, J. Alocally advanced primary gastric lymphoma invading spleen, pancreas and transverse colon. In: 10th International Gastric Cancer Congress. Verona: International Gastric Cancer Association. 2013, p. 217–217.

30. MAREK, J., SOUKUP, O., CABAL, J., KUČA, K. Development of novel quaternary detergents as part of decontamination and disinfection means. Zjazd Chemikov. 2013, 9100. ISSN 1336-7242.

31. MISÍK, J. Military incapacitating agent BZ (3-quinuclidinyl benzilate) in behavioral research. Military Medical Science Letters. 2013, 82(suppl. 1), 21–22. ISSN 0372-7025.

32. PAVLÍK, V., HLÚBIK, P., FAJFROVÁ, J., NAVRÁTIL, P. Prevention of obesity in the Army of the Czech Republic. In: Sborník abstrakt: Prevence obezity v AČR. Teplice: Zdravotní ústav Ústí nad labem. 2013, p. 25. ISBN 978-80-905541-1-5.

33. POHANKA, M., ADAM, V., KIZEK, R. Electrochemical assay of butyrylcholinesterase in biological samples. In: 13. Pracovní setkání fyzikálních chemiků a elektrochemiků – sborník abstrakt. 2013, p. 11– 11.

34. POHANKA, M., ŠTĚTINA, R., SVOBODOVÁ, H., RUTTKAY-NEDECKÝ, B., JÍLKOVÁ, M., SOCHOR, J., SOBOTKA, J., ADAM, V., KIZEK, R. Sulfur mustard causes oxidative stress and depletion of antioxidants in organs of wistar rats. In: 37. Brněnské onkologické dny a 27. Konference pro nelékařské zdravotnické pracovníky. 2013, p. 156–156. ISBN 978-80-904596-9-4.

35. POHANKA, M. Modulation of immunization via cholinergic nervous system using HI-6. In: Journal Bacteriology and Infectious Diseases. 2013, p. 45–45.

36. PRYMULA, R., CHLÍBEK, R., KOSINA, P., SMETANA, J. Pneumococcal disease and new recommendation for adult immunization in the Czech Republic. In: Abstracts of the 2nd International Conference on Controversies in Vaccination in Adults (CoVAC). 2013, p. A-2.

37. RUTTKAY-NEDECKÝ, B., HYNEK, D., ŽUREK, M., MAŠKOVÁ, D., NEJDL, L., POHANKA, M., ADAM, V., KIZEK, R. Use of electrochemical methods for evaluation of critical health condition. In: 13. Pracovní setkání fyzikálních chemiků a elektrochemiků – sborník abstrakt. 2013, p. 114–114. ISBN 978-80-7375-757-1.

38. SMETANA, J., CHLÍBEK, R., GÁL, P., DÍTĚ, P., BOŠTÍKOVÁ, V., TRTÍLKOVÁ, M. Knowledge of the voluntary adult vaccination. Vakcinologie. 2013, 7(1), 33–33. ISSN 1802-3150.

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39. SMETANA, J., CHLÍBEK, R., BOŠTÍKOVÁ, V. Vaccination against tetanus in adulthood, new recommendations. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 80–80. ISBN 978-80-260-4939-5.

40. SMETANA, J., CHLÍBEK, R., BOŠTÍKOVÁ, V. Present vaccination against tetanus. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: České vakcinologická společnost ČLS JEP. 2013, p. 53–53. ISBN 978-80-260-4939-5.

41. SOUKUP, O., KORÁBEČNÝ, J., ŠPILOVSKÁ, K., MUSÍLEK, K., JUN, D., MISÍK, J., ŽĎÁROVÁ KARASOVÁ, J., KUČA, K. Hybrids of AChE inhibitor and memantine derivatives as candidates for Alzheimer´s disease treatment. In: Neuroscience 2013. 2013, p. 100.

42. SOUKUP, O., JUN, D., TOBIN, G., KUČA, K. Non-reactivating properties of oxime reactivators. In: Korean Chemical Society Meeting. 2013, p. 100.

43. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., ŠEPSOVÁ, V., DRTINOVÁ, L., KUČA, K. Synthesis and in vitro screening of 7-methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in Alzheimer's disease therapy. In: 14th International Symposium on Cholinergic Mechanisms. 2013, p. 2.

44. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., BENEK, O., DRTINOVÁ, L., ŠEPSOVÁ, V., KASSA, J., NEPOVIMOVÁ, E., KUČA, K. 7-methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in AD treatment – synthesis, biological evaluation and molecular docking. In: The 11th International Conference on Alzheimer's and Parkinson's disease. 2013, p. 1.

45. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., BENEK, O., KUČA, K. Synthesis and biological testing of 7- methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in Alzheimer's disease. In: Sborník příspěvků 55. česko-slovenské psychofarmakologické konference. Praha: Česká neuropsychofarmakologická společnost. 2013, p. 2.

46. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Measles – recurring problem in Europe. In: Sborník abstrakt 9. hradeckých vakcinologických dnů. Praha: Česká vakcinologická společnost. 2013, p. 45–45. ISBN 978-80-260-4939-5.

47. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Rare primary tumors – case reports. In: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu – sborník abstrakt. Plzeň: EUROVERLAG. 2013, p. 67. ISBN 978-80-7177-977-3.

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48. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Surgical treatment of liver metastases of colorectal cancer: analysis of predictive factors. In: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu – sborník abstrakt. Plzeň: EUROVERLAG. 2013, p. 60. ISBN 978-80-7177-977-3.

49. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Laparoscopic vs. open liver resection for metastatic colorectal cancer: oncological result. In: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu – sborník abstrakt. Plzeň: EUROVERLAG. 2013, p. 67. ISBN 978-80-7177-977-3.

50. ŽĎÁROVÁ KARASOVÁ, J., PAVLÍK, M., CHLÁDEK, J., JUN, D., KUČA, K. Oxime HI-6 salts (dichloride and dimethansulphonate): Hyaluronidase effect on biovaibility after i. m. application. Military Medical Science Letters. 2013, 82(suppl. 1), 39. ISSN 0372-7025.

22th Annual Report, 2013 – 131 PRESENTATIONS AND POSTERS PRESENTATIONS AND POSTERS

1. BALONOVÁ, L. Current knowledge of the Francisella tularensis glycobiology. Velká Úpa: Výroční zasedání ÚMP a CPS. 28.11.2013– 29.11.2013. 2. BALONOVÁ, L. Protein glycosylation in Francisella tularensis: identification of a novel glycosylated lipoprotein. Hradec Králové: 3. neformální proteomické setkání. 21.11.2013–22.11.2013. 3. BENEK, O., ŠPILOVSKÁ, K., KORÁBEČNÝ, J., KUČA, K., GUNN- MOORE, F., MUSÍLEK, K. Inhibitors of ABAD-Aβ interaction as potential AD treatment. Florence, Italy: 11th International Conference Alzheimer’s and Parkinson’s Diseases. 06.03.2013–10.03.2013. /POSTER/ 4. BOŠTÍK, P. CBRN defense and potential new threats from "common" technologies. Rio de Janeiro, Brasil: CBRN Threat Defense Seminar. 28.11.2013–30.11.2013. 5. BOŠTÍK, P., BOŠTÍKOVÁ, V. Identification and strain surveillance of clinical isolates of varicella-zoster virus (VZV) in Europe using molecular epidemiology. Wuhan, China: BIT`s 3rd Annual World Congress of Microbes. 30.07.2013–01.08.2013. 6. BOŠTÍKOVÁ, V., BOŠTÍK, P., PRÁŠIL, P., SALAVEC, M., SMETANA, J., CHLÍBEK, R., KOSINA, P., PLÍŠEK, S. Genetic diversity of Varicella zoster virus (VZV) in Europe focusing on Czech Republic – What´s now, and what´s next. Cape Town, RSA: 8th World Congress of the Society for Pediatric Infectious Diseas. 19.11.2013–22.11.2013. /POSTER/ 7. BOŠTÍKOVÁ, V., BOŠTÍK, P., PRÁŠIL, P., PLÍŠEK, S., SMETANA, J., CHLÍBEK, R. Breakthrough varicella-zoster infection after immunization, presenting as chickenpox in child with cystic fibrosis (CF). Cape Town, RSA: 8th World Congress of the Society for Pediatric Infectious Diseases. 19.11.2013–22.11.2013. /POSTER/ 8. ČÁP, R., ŠKOLOUDÍK, L. Surgical treatment of malignant tumors of the skin on head and neck. Hradec Králové: ORL kongres. 24.05.2013–24.05.2013. 9. ČÁP, R. Non-adherent covering for the vacuum treatment of wounds. Bedřichov u Liberce: Safetac Club. 04.10.2013–05.10.2013. 10. ČÁP, R., KLEIN, L. Paralysis of the facial nerve from the perspective of a plastic surgeon. Hradec Králové: Schůze České společnosti otorinolaryngologie a chirurgie hlavy a krku ČLS JEP Královéhradeckého a Pardubického regionu. 17.10.2013–17.10.2013.

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11. DAŇKOVÁ, V., BALONOVÁ, L., STULÍK, J. The ABC transporter is required for host cell adhesion, glycosylation, and virulence in the bacterium Francisella tularensis. Urgup, Turkey: International Symposium on Francisella tularensis and Tularemia. 19.06.2013– 22.06.2013. /POSTER/ 12. DOHNALOVÁ, L., DOHNAL, V., STŘÍTECKÁ, H. Comparison BIO versus nonBIO – current findings. Praha: Výživa, potraviny a zdraví – 5. studentská konference. 12.06.2013–12.06.2013. 13. DRESLER, J., RIEHM, J., PAJER, P., HUBÁLEK, M., KLIMENTOVÁ, J., FUČÍKOVÁ, A., MATĚJKOVÁ, J., SCHOLZ, H., PÍŠA, L. Detection and differentiation of BoNT by mass spectrometry. Munich, Germany: Medical Biodefense Conference 2013. 22.10.2013–25.10.2013. 14. DRTINOVÁ, L., DOBEŠ, P., POHANKA, M. Low molecular weight precursor applicable for Alzheimer disease drugs synthesis (AChE and BChE inhibition, BACE inhibition, antioxidant properties and in silico modulation). Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 15. DRTINOVÁ, L., POHANKA, M., KRAČMAROVÁ, A., ŠEPSOVÁ, V. Evaluation of chemical percursors for their ability to inhibit enzyme acetylcholinesterae. Lázně Jeseník: 55. česko-slovenská psychofarmakologická konference. 04.01.2013–08.01.2013. /POSTER/ 16. DRTINOVÁ, L., POHANKA, M., ŠPILOVSKÁ, K., KORÁBEČNÝ, J., ŠEPSOVÁ, V., KRAČMAROVÁ, A., KUČA, K., KASSA, J., NEPOVIMOVÁ, E., MISÍK, J. Comparison of potencial ability to inhibit beta-secretase with standard drugs for AD treatment. Florence, Italy: 11th international konference on Alzheimer´s and Parkinson´s diseases. 06.03.2013–10.03.2013. /POSTER/ 17. FABIÁNOVÁ, K., CHLÍBEK, R., SMETANA, J., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., LŽIČAŘOVÁ, D., MACHAČ, J. Seroprevalence of antibodies Bordetella pertussis in adults in the Czech Republic. Olomouc: Kongres klinické mikrobiologie, infekčních nemocí a epidemiologie. 17.10.2013–19.10.2013.

18. FABRIK, I., LINK, M., HÄRTLOVÁ, A., DAŇKOVÁ, V., ŘEHULKA, P., STULÍK, J. SILAC labeling reveals extensive GM-CSF-dependent arginine metabolism in primary bone marrow-derived dendritic cells. Hradec Králové: 3. Česká konference pro hmotnostní spektroemtrii. 16.10.2013–18.10.2013. /POSTER/

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19. FABRIK, I. Mass spectrometry: MaxQuant (data-processing software for Orbitrap). Hradec Králové: Interní výukový seminář laboratorních metod. 12.02.2013–26.02.2013. 20. FABRIK, I. GM-CSF-dependent arginine metabolism affects SILAC labeling in primary dendritic cells. Hradec Králové: 7. fakultní konference studentů doktorských programů. 01.10.2013–01.10.2013. 21. FAJFROVÁ, J. Therapy and prevention of obesity. Těchonín: Vojenská odborná příprava. 27.05.2013–27.05.2013. 22. FAJFROVÁ, J., PAVLÍK, V., BEDNARČÍK, P., HLÚBIK, P. Limitation of BMI for diagnosing overweight in Czech Army. Hradec Králové: Obezitologie a bariatrie 2013. 17.10.2013–19.10.2013. /POSTER/ 23. FAJFROVÁ, J. Nonalcoholic fatty liver disease. Praha: Školení lékařů spádu IV. 27.11.2013–27.11.2013. 24. FAJFROVÁ, J. Military hygiene and epidemiological service – activity in the branch of Ministry of Defence. Hradec Králové: Vstupní odborná příprava příslušníků zdravotnické a veterinární služby AČR. 13.11.2013– 14.11.2013. 25. FAJFROVÁ, J., PAVLÍK, V., BEDNARČÍK, P., HLÚBIK, P. Recommendation for diagnosing obesity in Czech Army. Kúpele Nový Smokovec, Slovakia: 21. vedecko-odborná konferencia s medziná- rodnou účasťou Životné podmienky a zdravie. 23.09.2013–25.09.2013. /POSTER/ 26. FAJFROVÁ, J. Improvement of fitness and health of military professionals – metabolic syndrom and nonalcoholic fatty liver disease. Hradec Králové: Vědecká rada FVZ. 18.09.2013–18.09.2013. 27. FAJFROVÁ, J. Therapy and prevention of obesity. Chrudim: Vojenská odborná příprava. 30.08.2013–30.08.2013. 28. FAJFROVÁ, J., PAVLÍK, V., BEDNARČÍK, P., HLÚBIK, P. Limitation of BMI for diagnosing overweight in Czech Army. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 29. FERKO, A., ŠUBRT, Z., ČEČKA, F., JON, B. Laparoscopic vs. open liver resection for metastatic colorectal cancer: oncological results. Ostrava: 10. Ostravské dny miniinvazivní chirurgie. 07.11.2013– 08.11.2013. 30. FERKO, A., ŠUBRT, Z. Two case reports: bile duct injury during cholecystectomy with an unusually complicated course. Are we able

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to learn? Karlovy Vary: 5. kongres České gastroenterologické společnosti. 07.11.2013–09.11.2013. 31. FINTORA, J., VANĚK, J., JEŽEK, B. Crowd rendering based on Makehuman™ data. Horní Lomná: Sborník příspěvků 33. konference o geometrii a grafice. 09.09.2013–12.09.2013. 32. FIŠER, V., PROCHÁZKA, M. Trauma planning on the level of regional government and emergfency medical service. Hradec Králové: Seminář Centra andragogiky. 17.01.2013–17.01.2013. 33. FUČÍKOVÁ, A., DRESLER, J., ŘEHULKOVÁ, H., ZÍDKOVÁ, L., LENČO, J., STULÍK, J., PUDIL, R. Potential plasma markers of hypertrophic cardiomyopathy – targeted proteomic analysis of soluble fibronectin. Yokohama, Japan: HUPO 2013. 12.09.2013–19.09.2013. /POSTER/ 34. FUČÍKOVÁ, A., DRESLER, J., JUN, D., KROČOVÁ, Z., MACELA, A., SCHMIDT, M., STULÍK, J. Targeted proteomic analysis of ricin. Munich, Germany: Medical Biodefence Conference. 22.10.2013–25.10.2013. /POSTER/ 35. GRÖGER, T. First aid in the field. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 36. HALAJČUK, T., PROCHÁZKA, M. Economy framework of the emer- gency medical service in the Czech Republic. Hradec Králové: Hradecké ekonomické dny. 19.02.2013–20.02.2013. 37. HALAJČUK, T., PROCHÁZKA, M. Selected cost indicators of pre- hospital emergency care in the Czech Republic. Sunny Beach, Bulgaria: International Scientific Symposium Economy & Business. 01.09.2013– 06.09.2013. 38. HALAJČUK, T., PROCHÁZKA, M. Trauma plan analysis. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013. 39. HALAJČUK, T., PROCHÁZKA, M. Economy frame of pre-hospital emergency care financing in the Czech Republic. Hradec Králové: Ekonomická efektivita v PNP? 27.11.2013–27.11.2013. 40. HAMULÁKOVÁ, S., ŠPILOVSKÁ, K., HRABINOVÁ, M., JANOVEC, L., KUČA, K., IMRICH, J. Syntesis and biological efficacy tacrine-coumarine analoges as potential Alzheimer´s drugs. Tatranské Matliare, Slovakia: 65. Zjazd chemikov. 09.09.2013–13.09.2013.

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41. HAVEL, F., NOVOTNÝ, F., JELÍNEK, M., PROŠKA, J., KUČA, K., MUSÍLEK, K. Optimalization of optothermal properties of gold nanorods. Brno: NANOCON 2013. 16.10.2013–18.10.2013. /POSTER/ 42. HLÚBIK, J., HLÚBIK, P., STŘÍTECKÁ, H. Use BIA analysis or basic anthropometrical parametres to evaluate the success of weight loss. Hradec Králové: Obezitologie a bariatrie 2013. 17.10.2013–19.10.2013. /POSTER/ 43. HLÚBIK, J., HLÚBIK, P., LHOTSKÁ, L. Changes in bioimpendance depending on conditions. Biała Podlaska, Poland: Higiena żywności i żywienia podstawą zdrowia. 03.09.2013–06.09.2013. 44. HLÚBIK, J., LHOTSKÁ, L., STŘÍTECKÁ, H., HLÚBIK, P. Changes in bioimpedance and body composition parameters depending on measurements conditions. Biała Podlaska, Poland: Higiena żywności i żywienia podstawą zdrowia. 03.09.2013–06.09.2013. 45. HLÚBIK, P., HLÚBIK, J., STŘÍTECKÁ, H. Modified polysaccharides – possibilities and limitations in influencing metabolic disorders. Špindlerův Mlýn: 17. kongres o ateroskleróze. 05.12.2013–07.12.2013. 46. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., ŽÁK, P., JEBAVÝ, L. Assessment of serum cytokine and adhesion molecule levels in patients with acute lymphoblastic leukemia using biochip array technology. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 47. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Serum concentrations of cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia. Olomouc: 27. Olomoucké hematologické dny s mezinárodní účastí. 12.05.2013– 14.05.2013. /POSTER/ 48. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., ŽÁK, P., JEBAVÝ, L. Evaluation of serum levels of adhesion molecules by biochip array technology in acute myeloid leukemia patients. Milan, Italy: 20th IFCC- EFLM European Congress of Clinical Chemistry and Laboratory Medicine (EuroMedLab). 19.05.2013–23.05.2013. /POSTER/

49. HORÁČEK, J., JEBAVÝ, L., VAŠATOVÁ, M., TICHÝ, M., ŽÁK, P. Assessment of cardiotoxicity induced by conventional and high-dose chemotherapy for acute leukemia with multiple biomarkers of cardiac injury. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 50. HORÁČEK, J., CERMANOVÁ, M., ZAVŘELOVÁ, A., LÁNSKÁ, M., JEBAVÝ, L., ŽÁK, P. Treatment of adult acute lymphoblastic leukemia

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according to GMALL 07/2003 protocol – single center experience. Olomouc: 27. Olomoucké hematologické dny s mezinárodní účastí. 12.05.2013–14.05.2013. 51. HORÁČEK, J., ŽÁK, P., KUPSA, T., JAKL, M., BRNDIAR, M., JEBAVÝ, L. Acute lymphoblastic leukemia – innovations in diagnostics and treatment. Brno: 59. konference vojenských internistů. 17.10.2013– 18.10.2013. 52. HORÁČEK, J., VAŠATOVÁ, M., PUDIL, R., ŽÁK, P., JEBAVÝ, L. The use of cardiac biomarkers in the detection of cardiotoxicity associated with stem cell transplantation for hematological malignancies. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 53. HORÁČEK, J., VAŠATOVÁ, M., PUDIL, R., TICHÝ, M., JAKL, M., JEBAVÝ, L. Cardiac biomarkers and assessment of cardiotoxicity in patients treated for acute leukemia. Hradec Králové: 13. konference Odborné společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 54. HORÁČEK, J., JEBAVÝ, L., VAŠATOVÁ, M., ŽÁK, P., TICHÝ, M., MALÝ, J. Multiple biomarkers in the detection of cardiotoxicity induced by conventional and high-dose chemotherapy for acute leukemia. Istanbul, Turkey: 4th International Congress on Leukemia Lymphoma Myeloma. 22.05.2013–25.05.2013. /POSTER/ 55. HORÁČEK, J. Acute leukamias on ASH 2012. Hradec Králové: Hematologický seminář 4. interní hematologické kliniky FN Hradec Králové. 23.01.2013–23.01.2013. 56. HORÁČEK, J. Treatment outcomes of acute lymphoblastic leukemia in University Hospital Hradec Kralove. Hradec Králové: Hematologický seminář 4. interní hematologické kliniky FN Hradec Králové. 20.02.2013–20.02.2013. 57. HORÁČEK, J., KUPSA, T., VAŠATOVÁ, M., ŽÁK, P., JEBAVÝ, L. Serum cytokine and adhesion molecule levels in patients with acute lymphoblastic leukemia and in healthy subjects. Istanbul, Turkey: 4th International Congress on Leukemia Lymphoma Myeloma. 22.05.2013– 25.05.2013. /POSTER/ 58. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., ŽÁK, P., JEBAVÝ, L. Evaluation of serum cytokine and adhesion molecule concentrations in patients treated for acute myeloid leukemia. Brno: 37. Brněnské onkologické dny a 27. Konference pro nelékařské zdravotnické pracovníky. 18.04.2013–19.04.2013.

22th Annual Report, 2013 – 137 PRESENTATIONS AND POSTERS

59. HORÁČEK, J., VAŠATOVÁ, M., KUPSA, T., JEBAVÝ, L., ŽÁK, P. Serum levels of multiple cytokines and adhesion molecules in patients treated for acute myeloid leukemia. Istanbul, Turkey: 4th International Congress on Leukemia Lymphoma Myeloma. 22.05.2013–25.05.2013. /POSTER/ 60. HORÁČEK, J., VAŠATOVÁ, M., PUDIL, R., JAKL, M., ŽÁK, P., JEBAVÝ, L. Multiple biomarkers in the detection of cardiotoxicity during stem cell transplantation for hematological malignancies. Istanbul, Turkey: 4th International Congress on Leukemia Lymphoma Myeloma. 22.05.2013–25.05.2013. /POSTER/ 61. HOSTÝNKOVÁ, V. Identification of immunogenic epitope of microbe Francisella tularensis. Velká Úpa: Výroční zasedání ÚMP a CPS za rok 2013. 28.11.2013–29.11.2013. 62. HOSTÝNKOVÁ, V., STRAŠKOVÁ, A., LINK, M., STULÍK, J. Establishment of in vitro infection of mouse BMDC with F. tularensis strain LVS. Brno: The Student Scientific Conference on Biotechnology and Biomedicine 2013. 10.04.2013–12.04.2013. /POSTER/ 63. HRSTKA, Z. Burn out syndrome by healthcare workers during peacekeeping missions. Praha: 5. kongres anestezie a intenzivní péče za mimořádných podmínek. 17.10.2013–18.10.2013. 64. HRSTKA, Z. Critical incident stress management. Hradec Králové: 19. královéhradecké ošetřovatelské dny. 12.09.2013–13.09.2013. /POSTER/ 65. HRSTKA, Z. Differences of perception between social workers by gender. Hradec Králové: Hradecké dny sociální práce. 27.09.2013– 28.09.2013. 66. HRSTKA, Z. Differences in perception quality of life females versus males. Hradec Králové: 7. konference všeobecných sester a radio- logických asistentů. 15.03.2013–15.03.2013. 67. HRSTKA, Z. Ethics and communication. Hradec Králové: Regionální seminář onkologů. 09.10.2013–09.10.2013. 68. HRSTKA, Z. Psychology and coping with cancer. Hradec Králové: Regionální seminář onkologů. 11.04.2013–11.04.2013. 69. HRSTKA, Z. Communication with patients. Tábor: Konference praktických lékařů. 23.01.2013–23.01.2013. 70. HRSTKA, Z. Pain control by psychological means. Hradec Králové: 19. královéhradecké ošetřovatelské dny. 12.09.2013–13.09.2013. /POSTER/

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71. HRSTKA, Z. Psychological aspects of dying and death. Hradec Králové: Regionální seminář onkologů. 14.01.2013–14.01.2013. 72. HRSTKA, Z. Health psychology. Praha: Vzdělávací seminář lékařů. 04.02.2013–04.02.2013. 73. HRSTKA, Z., SLOVÁČEK, L. The support system of health care professionals exposed to excess stress load. Hradec Králové: Vzdělávací programy pro zdravotnické profesionály. 23.04.2013– 23.04.2013. 74. HRSTKA, Z. Psychology of health and illness. Rychnov nad Kněžnou: Seminář k problémům dětí s handicapem. 12.11.2013–12.11.2013. 75. HRSTKA, Z. Aggression. Brno: 4. psychoonkologické sympozium. 11.09.2013–11.09.2013. 76. HRSTKA, Z. Experiencing the quality of life – women versus men. Praha: 5. kongres anestezie a intenzivní péče za mimořádných podmínek. 17.10.2013–18.10.2013. 77. HUMLÍČEK, V. Medical service organization and control under the terms of physicians professional education. Praha: Konference hlavních odborníků vojenské zdravotnické služby. 26.11.2013–26.11.2013. 78. HUMLÍČEK, V. Professional education of physicians in the area of military medical service control. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. 79. CHLÍBEK, R. Vaccination of adult patients and recommendations for vaccination against pneumococcal infection. Praha: Astma a CHOPN. 23.01.2013–23.01.2013. 80. CHLÍBEK, R. Prophylaxis and treatment of bio accident victims. Vyškov: JCBRN Defence Centre of Excellence – Crisis Management after CBRN Incident/Pilot Course. 11.11.2013–15.11.2013. 81. CHLÍBEK, R. Vaccination guidelines, vaccination calendar 2013. Jihlava: Očkování z celoživotního pohledu v ordinaci praktického lékaře. 05.06.2013–05.06.2013. 82. CHLÍBEK, R. Multiconsensual view in vaccination. Praha: Wonca 2013. 25.06.2013–29.06.2013. 83. CHLÍBEK, R. Tick-borne encephalitis – prevention. Pardubice: Český rozhlas Pardubice – pořad Máme hosty. 23.04.2013–23.04.2013.

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84. CHLÍBEK, R. A first responders perspective to bio-incidents. Vyškov: JCBRN Defence Centre of Excellence – Crisis Management after CBRN Incident/Pilot Course. 11.11.2013–15.11.2013. 85. CHLÍBEK, R. Vaccination of adult patients and recommendations for vaccination against pneumococcal infection. Zlín: Astma a CHOPN. 07.01.2013–07.01.2013. 86. CHLÍBEK, R. Vaccination of adult patients and recommendations for vaccination against pneumococcal infection. Ústí nad Labem: Astma a CHOPN. 10.01.2013–10.01.2013. 87. CHLÍBEK, R. Adult vaccination and recommendation for pneumococcal vacination. Ostrava: Úskalí praktické medicíny. 15.05.2013–15.05.2013. 88. CHLÍBEK, R. Vaccination guidelines, vaccination calendar 2013. Ústí nad Labem: Očkování z celoživotního pohledu v ordinaci praktického lékaře. 17.09.2013–17.09.2013. 89. CHLÍBEK, R. Optional vaccination from a vaccinologist´s point of view. České Budějovice: Celorepublikový cyklus vzdělávacích seminárů pro praktické lékaře pro děti a dorost na téma Očkování. 14.11.2013– 14.11.2013. 90. CHLÍBEK, R., SMETANA, J., ŠOŠOVIČKOVÁ, R. Analysis of epidemic cholera in Haiti – a lesson for the present. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013. 91. CHLÍBEK, R. Trends in adult vaccination and the introduction of new vaccines. Štrbské Pleso, Slovakia: 4. Slovenský vakcinologický kongres. 17.01.2013–19.01.2013. 92. CHLÍBEK, R. Interdisciplinary approach – vaccination in high-risk patients. Praha: 7. jarní interaktivní konference SVL ČLS JEP. 12.04.2013–14.04.2013. 93. CHLÍBEK, R. Optional vaccination from a vaccinologist´s point of view. Ostrava: Celorepublikový cyklus vzdělávacích seminárů pro praktické lékaře pro děti a dorost na téma Očkování. 12.09.2013–12.09.2013.

94. CHLÍBEK, R., SMETANA, J., FABIÁNOVÁ, K., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., LŽÍČAŘOVÁ, D., BOŠTÍKOVÁ, V., ŠPLIŇO, M. The seroepidemiology of Bordetella pertussis in the Czech Republic. Cape Town, RSA: 8th World Congress of the Society for Pediatric Infectious Diseases. 19.11.2013–22.11.2013. /POSTER/

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95. CHLÍBEK, R. Optional vaccination from a vaccinologist´s point of view. Plzeň: Celorepublikový cyklus vzdělávacích seminárů pro praktické lékaře pro děti a dorost na téma Očkování. 10.09.2013–10.09.2013. 96. CHLÍBEK, R. Vaccination of adult patients and recommendations for vaccination against pneumococcal infection. Ostrava: Astma a CHOPN. 22.01.2013–22.01.2013. 97. CHLÍBEK, R. Vaccination and public health. Bratislava, Slovakia: 10. slovenský pediatrický kongres. 25.04.2013–27.04.2013. 98. CHLÍBEK, R. Difficulties in practical medicine. Ostrava: Seminář praktických lékařů. 15.05.2013–15.05.2013.

99. CHLÍBEK, R., SMETANA, J., FABIÁNOVÁ, K., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., LŽÍČAŘOVÁ, D. Seroprevalence of Bordetella pertussis in adults in the Czech Republic. Brno: Český kongres o infekčních nemocech. 25.09.2013–27.09.2013. 100. CHLÍBEK, R. Vaccination guidelines, vaccination calendar 2013. Praha: Očkování z celoživotního pohledu v ordinaci praktického lékaře. 11.09.2013–11.09.2013. 101. CHLÍBEK, R. Vaccination guidelines, vaccination calendar 2013. Brno: Očkování z celoživotního pohledu v ordinaci praktického lékaře. 24.09.2013–24.09.2013. 102. CHLÍBEK, R. Adult vaccination and recommendation for pneumococcal vaccination. Brno: Úskalí praktické medicíny. 06.06.2013–06.06.2013. 103. CHLÍBEK, R. Vaccination guidelines, vaccination calendar 2013. Hradec Králové: Očkování z celoživotního pohledu v ordinaci praktického lékaře. 10.06.2013–10.06.2013. 104. CHLÍBEK, R., SMETANA, J. Multidisciplinary approaches to vaccination. Praha: 20th World Conference Family Medicine – Care for Generations, Wonca 2013. 25.06.2013–29.06.2013. 105. CHLÍBEK, R., MACHAČ, J., SMETANA, J., FABIÁNOVÁ, K., ZAVADILOVÁ, J., DÍTĚ, P., GÁL, P., NÁPLAVA, P., LŽÍČAŘOVÁ, D. The results of seroprevalence studies of pertussis in the Czech Republic – influence vaccination strategy. Hradec Králové: 9. Hradecké vakcinologické dny. 03.10.2013–05.10.2013. /POSTER/ 106. CHLÍBEK, R. News vaccination in 2013. Ústí nad Labem: Konference Co nového v očkování v roce 2013 – pro všeobecné sestry ordinací lékařů pro děti a dorost. 07.12.2013–07.12.2013.

22th Annual Report, 2013 – 141 PRESENTATIONS AND POSTERS

107. CHLÍBEK, R. Update on pertussis. Belgrade, Serbia: Central and Eastern Europe Pertussis Awareness Group. 30.01.2013–31.01.2013. 108. CHLÍBEK, R. The vaccination schedule for adults. Praha: Očkování v dospělosti. 15.04.2013–15.04.2013. 109. CHLÍBEK, R. Vaccinate? Olomouc: 48th International Festival of Science Documentary Films. 16.04.2013–21.04.2013. 110. CHLÍBEK, R., SMETANA, J., BOŠTÍKOVÁ, V., ŠPLIŇO, M. Adult vaccination – recommendation. Poprad, Slovakia: 17. Slovensko-Český kongres o infekčních chorobách. 13.06.2013–15.06.2013. 111. CHLÍBEK, R. Priorities for vaccination in 2013 from the perspective of GPs. Olomouc: 11. kongres praktických lékařů a sester. 25.04.2013– 26.04.2013. 112. JAKL, M., MALÝ, J., FÁTOROVÁ, I., HORÁČEK, J., PUDIL, R. High on- treatment platelet reactivity: not an isolated disorder. Stockholm, Sweden: 18th Congress of EHA. 13.06.2013–16.06.2013. /POSTER/ 113. JAKL, M., FÁTOROVÁ, I., HORÁČEK, J. The failure of antiplatelet therapy in the context of other disorders of haemostasis. Brno: 59. konference vojenských internistů. 17.10.2013–18.10.2013. 114. JAKL, M., BIS, J., HORÁČEK, J. Evalution of the effect of balloon aortic valvuloplasty. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 115. JANEČKA, P., PETRÁNEK, K., JEŽEK, B., VANĚK, J. Implicit surface visualization using Raymarching. Horní Lomná: 33. konference o geometrii a grafice. 09.09.2013–12.09.2013. 116. JANOČKOVÁ, J., GULASOVA, Z., PLSIKOVA, J., MUSÍLEK, K., KUČA, K., MIKES, J., CULKA, L., FEDOROCKO, P., KOZURKOVA, M. Biological activity of cholinesterase modulators and their interactions with DNA. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 117. JAREŠOVÁ, L. A historical view of burn treatment. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013. /POSTER/ 118. JAREŠOVÁ, L. Operation of the Czechoslovak field hospital in Korea during the period 1952–1954. Brno: Po stopách zdraví a nemoci člověka a zvířat. 24.09.2013–25.09.2013. /POSTER/

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119. JAREŠOVÁ, L. Czechoslovak medical help in Korea during the period 1952–1954. Praha: Pracovní den sester. 16.05.2013–16.05.2013. 120. JAREŠOVÁ, L. Czechoslovak military medical help in war conflicts during the 20th century – The Korean War. Praha: 5. kongres Anestézie a intenzivní péče za mimořádných podmínek. 17.10.2013–18.10.2013. 121. JAREŠOVÁ, L. A historical view of burn treatment. Hradec Králové: 2. celostátní Královéhradecký kongres perioperačních sester a chirur- gických intenzivistů. 25.09.2013–26.09.2013. 122. JAREŠOVÁ, L. Women active participation in army medical corps during the World War II. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. 123. JAREŠOVÁ, L. A historical view of burn treatment. Praha: 5. kongres Anestézie a intenzivní péče za mimořádných podmínek. 17.10.2013– 18.10.2013. /POSTER/ 124. JAREŠOVÁ, L. Operation of the Czechoslovak field hospital in Korea during the period 1952 – 1954. Praha: 5. kongres Anestézie a intenzivní péče za mimořádných podmínek. 17.10.2012–18.10.2013. /POSTER/ 125. JEBAVÝ, L., KAČEROVSKÝ, J., ZAVORAL, M., BRABEC, T., VYHNÁNEK, M., HALATA, P., HŘÍBEK, M. Summary of Activities in the field of internal medicine in the Medical Service of the ACR for the year 2012. Brno: 59. konference vojenských internistů. 17.10.2013–18.10.2013. 126. JEBAVÝ, L. Military Internal Medicine, view main expert of medical Service ACR. Hradec Králové: 13. konference Odborné společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. 127. JEŽEK, B., VANĚK, J., PROCHÁZKA, M. Major incident simulation. Pardubice: SW nástroje na řešení úloh racionalizace, objektivizace a optimalizace (nejen) pokrytí území veřejnými obslužnými systémy. 28.11.2013–29.11.2013. 128. JEŽEK, B., VANĚK, J., PETRÁNEK, K. Space in computer graphics. Hradec Králové: Letní škola FIM UHK. 13.05.2013–16.05.2013. 129. JEŽEK, B., VANĚK, J., PROCHÁZKA, M., HALAJČUK, T., PETRÁNEK, K. Simulation and GIS in major incident preparation. Praha: Konference GIS Esri v ČR. 13.11.2013–14.11.2013. /POSTER/

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130. JEŽEK, B., VANĚK, J. Heuristic label placement in image. Horní Lomná: Sborník příspěvků 33. konference o geometrii a grafice. 09.09.2013– 12.09.2013. 131. JOŠT, P., FIKROVÁ, P., JÍLKOVÁ, M. Detection of alkylation DNA damage induced sulphur mustard and 2- chlorethyl ethyl sulphide using comet assay. Porto, Portugal: The 10th International Comet Assay Workshop. 18.09.2013–20.09.2013. /POSTER/ 132. JUN, D., KŘENKOVÁ, Z., MUSÍLEK, K., KUČA, K. In vitro oxime- assisted reactivation of human butyrylcholinesterase – development of potential prophylaxis for nerve agent intoxications. Cavtat, Croatia: CSCM – World Congress on CBRN Science & Consequence Management. 14.04.2013–19.04.2013. 133. KASSA, J., ŽĎÁROVÁ KARASOVÁ, J., ŠTĚTINA, R., BAJGAR, J. The benefit of combinations of acetylcholinesterase reactivators for the antidotal treatment of poisonings with cyclosarin. Munich, Germany: 14th International Medical Chemical Defence Conference. 24.04.2013–25.04.2013. /POSTER/ 134. KASSA, J., MISÍK, J., ŽĎÁROVÁ KARASOVÁ, J. A comparison of neuroprotective efficacy of a novel oxime K203 with obidoxime and the oxime HI-6 in sarin-poisoned rats. Lázně Jeseník: 55. česko- slovenská psychofarmakologická konference. 04.01.2013–08.01.2013. /POSTER/ 135. KASSA, J., ŠEPSOVÁ, V., TŮMOVÁ, M., HOROVÁ, A., MUSÍLEK, K. The evaluation of the reactivating and therapeutic efficacy of two newly developed oximes K361 and K378 in comparison with the oxime K203 and trimedoxime in tabun-poisoned rats and mice. Olomouc: 63. Česko- Slovenské farmakologické dny. 11.09.2013–13.09.2013. /POSTER/ 136. KASSA, J. Antidotes against chemical warfare agents in the Czech Army – presence and future. Brno: 66th CIOR Congress and the 67th CIOMR Congress. 30.07.2013–02.08.2013. 137. KASSA, J., KORÁBEČNÝ, J., ŠEPSOVÁ, V., TŮMOVÁ, M., HOROVÁ, A., MUSÍLEK, K. The development of modulators of cholinesterases and the evaluation of their reactivating or inhibiting efficacy by the in vitro and in vivo methods. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 138. KASSA, J., ŠEPSOVÁ, V., MISÍK, J., ŽĎÁROVÁ KARASOVÁ, J., KUČA, K., MUSÍLEK, K., HOROVÁ, A., BAJGAR, J. The reactivating, therapeutic and neuroprotective efficacy of two newly developed oximes (K456, K458) in rats and mice poisoned with tabun – a comparison

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with the oxime K203 and trimedoxime. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. 139. KASSA, J., MISÍK, J. Cognitive deficit induced by cholinergic antagonist 3-quinuclidinyl benzilate – potential pharmacological model of Alzheimer disease. Florence: AD/PD™ 2013: The 11th International Conference on Alzheimer’s and Parkinson’s Diseases. 06.03.2013–10.03.2013. /POSTER/ 140. KLEIN, L., HAVEL, E., ŠMEJKAL, K., ZAJÍČEK, R. Combination of mechanical and burns injuries. Hanoi, Vietnam: The 9th Asia – Pacific Burn Congress. 02.04.2013–05.04.2013. 141. KLEIN, L. Procedures for management of delivery of humanitarian assistance to civilian populations in times of conflict – the Czech experience. Vienna, Austria: 15th European Burns Association Congress. 28.08.2013–31.08.2013. 142. KLIMENTOVÁ, J. Ultrafiltration and concentration of protein samples by Millipore Stirred Ultrafiltration Cell. Hradec Králové: Interní výukový seminář laboratorních metod. 19.02.2013–19.02.2013. 143. KLIMENTOVÁ, J. Analysis of protein complexes by Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE). Hradec Králové: Interní výukový seminář laboratorních metod. 19.02.2013–19.02.2013. 144. KLIMENTOVÁ, J. Outer membrane vesicles of F. tularensis. Velká Úpa: Výroční zasedání ÚMP a CPS. 28.11.2013–29.11.2013. 145. KORÁBEČNÝ, J., MUSÍLEK, K., BABKOVÁ, K., SOUKUP, O., HOROVÁ, A., ŠPILOVSKÁ, K., ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., KASSA, J., JUN, D., KUČA, K. Centrally and peripherally active Ache inhibitors – prophylaxis against nerve agents. Cavtat, Croatia: CSCM – World Congress on CBRNe Science & Consequence Management. 14.04.2013–19.04.2013. 146. KOSINA, P., SMETANA, J. Vaccination in questions and answers – parent view. Brno: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 30.10.2013–30.10.2013. 147. KOSINA, P., SMETANA, J. Questions and answers on the practice pediatrician. České Budějovice: Vzdělávací seminář pro PLDD na téma Očkování. 04.11.2013–04.11.2013. 148. KOSINA, P., SMETANA, J. Questions and answers on the practice pediatrician. Hradec Králové: Celorepublikový cyklus vzdělávacích seminářů pro praktické lékaře pro děti a dorost na téma Očkování. 18.06.2013–18.06.2013.

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149. KOSINA, P., SMETANA, J. Vaccination in questions and answers – parent view. Ostrava: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 12.11.2013– 12.11.2013. 150. KOSINA, P., SMETANA, J. Questions and answers on the practice pediatrician. Brno: Vzdělávací seminář pro PLDD na téma Očkování. 23.05.2013–23.05.2013. 151. KOSINA, P., SMETANA, J. Questions and answers on the practice pediatrician. Ostrava: Vzdělávací seminář pro PLDD na téma Očkování. 12.09.2013–12.09.2013. 152. KOSINA, P., SMETANA, J. Vaccination in questions and answers – parent view. Plzeň: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 20.11.2013–20.11.2013. 153. KRAČMAROVÁ, A., POHANKA, M. Cognitivum tacrine influences oxidative balance of organism. Lázně Jeseník: 55. česko-slovenská psychofarmakologická konference. 04.01.2013–08.01.2013. /POSTER/ 154. KRAČMAROVÁ, A., POHANKA, M., PIKULA, J., BANĎOUCHOVÁ, H., DRTINOVÁ, L., ŠEPSOVÁ, V., ŠPILOVSKÁ, K. Effect of tacrine on oxidative stress and plasma biochemistry in laboratory Guinea pig model. Florence, Italy: AD/PD™ 2013: The 11th International Conference on Alzheimer’s and Parkinson’s Diseases. 06.03.2013– 10.03.2013. /POSTER/ 155. KRAČMAROVÁ, A., MISÍK, J., KASSA, J., POHANKA, M. Immunomodulating effect of selected neurodrugs on murine macrophage cell line j774.2. Barcelona, Spain: 26th ECNP Congress. 05.10.2013–10.10.2013. /POSTER/ 156. KRAČMAROVÁ, A., ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., BAJGAR, J. In vitro testing of HI-6 skin permeation. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 157. KRAČMAROVÁ, A., PAVLIŠ, O., DRTINOVÁ, L., POHANKA, M. Immunomodulating effect of selected drugs due to cholinergic antiinflammatory pathway. Brno: 2. Květinův den. 23.05.2013– 23.05.2013. 158. KROČOVÁ, Z., KUBELKOVÁ, K., PLZÁKOVÁ, L., ZÁRYBNICKÁ, L., ŠINKOROVÁ, Z., MACELA, A. The B cells and innate immune response against intracellular bacterial pathogen Francisella tularensis. Mikulov: International Conference Analytical Cytometry VII. 21.09.2013– 24.09.2013. /POSTER/

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159. KROČOVÁ, Z., KUBELKOVÁ, K., PLZÁKOVÁ, L., ZÁRYBNICKÁ, L., ŠINKOROVÁ, Z., MACELA, A. The role of B cells in the early stages of immune response against intracellular bacterial pathogen Francisella tularensis. Pultusk, Poland: B Cells from Bedside to Bench and Back Again. 02.09.2013–07.09.2013. /POSTER/ 160. KROČOVÁ, Z., KUBELKOVÁ, K., PLZÁKOVÁ, L., ZÁRYBNICKÁ, L., ŠINKOROVÁ, Z., MACELA, A. B cells participate on the early stages of immune response against intracellular bacterial pathogen Francisella tularensis. Milan, Italy: 15th International Congress of Immunology. 22.08.2013–27.08.2013. 161. KUBELKOVÁ, K. PathScan antibody arrays and its application. Hradec Králové: Nové pohledy na účinek ionizujícího záření na živý organismus. 11.12.2013–11.12.2013. 162. KUBELKOVÁ, K. Effect of the ionizing radiation on the course of infectious diseases. Malá Úpa: Výroční zasedání ÚMP a CPS. 28.11.2013–29.11.2013. 163. KUBELKOVÁ, K., ORLÍKOVÁ, A., KROČOVÁ, Z., MACELA, A., PEJCHAL, J., BOŠTÍK, P. Humoral immunity involving antibodies and B cells as an outstaning helper during experimental Francisella tularensis infection. Matrahaza, Hungary: Impulse 2013 – EFIS-EJI Symposium. 31.08.2013–04.09.2013. /POSTER/ 164. KUČA, K., SOUKUP, O. Development of skin decontamination means based on micellar origin. Daejon, South Korea: Seminar of Korean Research Institute of Chemical Technology. 15.10.2013–18.01.2013. 165. KUČA, K. From the synthesis of new molecule to its practical application. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. 166. KUČA, K., MUSÍLEK, K., JUN, D., ŽĎÁROVÁ KARASOVÁ, J., KUNEŠ, M., SOUKUP, O., KORÁBEČNÝ, J., ŠEPSOVÁ, V., HRABINOVÁ, M. Novel acetylcholinesterase reactivators – antidotes against nerve agents. Singapore: Conference on Pharmacology and Drug Development. 09.12.2013–11.12.2013. /POSTER/ 167. KUČA, K., SOUKUP, O. Development of novel nerve agent antidotes in Czech Republic – prophylaxis and treatment. Daejon, South Korea: Seminar of Korean Research Institute of Chemical Technology. 13.10.2013–18.10.2013. 168. KUČA, K., JUN, D., MUSÍLEK, K. Development of novel cholinesterase reactivators – Czech approach. Paris, France: European Workshop on Reactivators of Inhibited Cholinesterases. 06.11.2013–07.11.2013.

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169. KUNEŠ, M., ŽĎÁROVÁ KARASOVÁ, J., ZEMEK, F., KVĚTINA, J., PAVLÍK, M., JUN, D., BUREŠ, J., KUČA, K. Detection of acetylcholinesterase reacti-vators in biological samples: pharmacokinetics of oximes hi-6 and K027 in pig. Velké Karlovice: Syntéza a analýza léčiv. 02.09.2013–05.09.2013. /POSTER/ 170. KUNEŠ, M., MUSÍLEK, K., JUN, D., ŽĎÁROVÁ KARASOVÁ, J., KASSA, J., SOUKUP, O., HRABINOVÁ, M., KUČA, K. Acetylcholinesterase reactivators and surfactants with a promising application potential. Cavtat, Croatia: CSCM – World Congress on CBRNe Science & Consequence Management. 14.04.2013–19.04.2013. 171. KUPSA, T., HORÁČEK, J., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Evaluation of serum concentrations of multiple cytokines and adhesion molecules in patients with newly diagnosed acute myeloid leukemia. Olomouc: 27. Olomoucké hematologické dny s mezinárodní účastí. 11.05.2013–14.05.2013. /POSTER/ 172. KUPSA, T., HORÁČEK, J., JEBAVÝ, L. Transplantation of hematopoietic stem cell – very general. Brno: 59. konference vojenských internistů. 17.10.2013–18.10.2013. 173. KUPSA, T. MDS/MPS and AML on EHA 2013. Hradec Králové: Hematologický seminář 4. interní hematologické kliniky FN Hradec Králové. 26.06.2013–26.06.2013. 174. KUPSA, T., HORÁČEK, J. Analysis of selected prognostic factors in patients with acute myeloid leukemia – the influence of cytokines and adhesion molecules. Hradec Králové: 7. konference PGS studentů. 01.10.2013–01.10.2013. 175. KUPSA, T., HORÁČEK, J. Evaluation of serum levels of multiple cytokines and adhesion molecules in patients treated for acute myeloid leukemia. Hradec Králové: 10. mezinárodní konference PGS studentů. 21.11.2013–22.11.2013. 176. KUPSA, T., VOGLOVÁ, J. Patient J. J., 1953. Deštné v Orlických horách: Regionální hematologické setkání. 04.10.2013–04.10.2013.

177. KUPSA, T., HORÁČEK, J., VAŠATOVÁ, M., JEBAVÝ, L., ŽÁK, P. Serum concentrations of selected cytokines and adhesion molecules in patients treated for acute myeloid leukemia. Hradec Králové: 13. konference Odborné společnosti lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 178. LENČO, J. Advanced analyses of proteins using mass spectrometry. Hradec Králové: Letní škola LFHK – 2013: Vybrané metodické přístupy v biomedicínském výzkumu a jejich aplikace. 10.06.2013–11.06.2013.

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179. LENČO, J., TAMBOR, V., VAJRYCHOVÁ, M., KACEROVSKÝ, M. PPROM: diagnostic potential of proteomic markers cathelicidin and myeloperoxidase in infection and inflammation. Praha: 13. Konference fetální medicíny ČLS JEP. 11.01.2013–20.12.2013. 180. LINK, M. Current technology status and apllications on nLC-Q-Orbi. Velká Úpa: Výroční zasedání ÚMP a CPS. 28.11.2013–29.11.2013. 181. MAČEK, N., RADIC, Z., TAYLOR, P., KUČA, K., KOVARIK, Z. Structural analogs of the HI-6 oxime are the most potent reactivators of the soman- inhibited AChE mutant that resists ageing. Munich, Germany: 14th International Medical Chemical Defence Conference. 24.04.2013– 25.04.2013. /POSTER/ 182. MACHAČ, J., CHLÍBEK, R., FABIÁNOVÁ, K., PLÍŠEK, S. Reported and actual incidence of pertussis – access to vaccination strategy. Olomouc: Kongres klinické mikrobiologie, infekčních nemocí a epidemiologie. 17.10.2013–19.10.2013. /POSTER/ 183. MALIŠOVÁ, Z., PAVLÍK, V. Coldness and woddcutters hob. Nový Smokovec, Slovakia: Životné podmienky a zdravie. 23.09.2013– 25.09.2013. /POSTER/ 184. MAREK, J., SOUKUP, O., CABAL, J., KUČA, K. Development of novel quaternary detergents as part of decontamination and disinfection means. Tatranské Matliare, Slovakia: Zjazd Chemikov. 09.09.2013– 13.10.2013. /POSTER/ 185. MAREK, J., JUN, D., KUČA, K., BARTOŠOVÁ, L. Preparation of new cationic surfactants and their disinfection efficiency evaluation. Krems, Austria: 4th International Congress Nanotechnology, Medicine and Biology. 13.03.2013–15.03.2013. 186. MATOUŠEK, R. Military acute care, BATLS philosophy. Praha: IPVZ. 14.05.2013–14.05.2013. 187. MATOUŠEK, R. BATLS in the Army of the Czech Republic. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 188. MISÍK, J. Effects of anticholinergic agents on learning and memory in the laboratory rat. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 189. MISÍK, J., SOUKUP, O., MUSÍLEK, K., KUČA, K., KASSA, J. Therapeutic effect of cholinesterase inhibitors rivastigmine, donepezil and tacrine on cognitive deficit induced by 3-quinuclidinyl benzilate

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in rats performing passive avoidance test. San Diego, USA: Neuroscience 2013. 08.11.2013–13.12.2013. /POSTER/ 190. MISÍK, J., KASSA, J., ŠEPSOVÁ, V., KORÁBEČNÝ, J., DRTINOVÁ, L. Therapeutic effect of tacrine, rivastigmine and donepezil on cognitive deficit induced by 3-quinuclidinyl benzilate in rats performing the passive avoidance task. Florence, Italy: AD/PD™ 2013: The 11th International Conference on Alzheimer’s and Parkinson’s Diseases. 06.03.2013– 10.03.2013. /POSTER/ 191. MISÍK, J. Military incapacitating agent BZ (3-quinuclidinyl benzilate) in behavioral research. Hradec Králové: 18th Interdisciplinary Czech- Slovak Toxicology Conference (TOXCON 2013). 19.06.2013– 21.06.2013. 192. MISÍK, J., KASSA, J. Impact of cholinergic antagonist 3-guinuclidinyl benzilate on spatial learning and memory in the laboratory rat. Lázně Jeseník: 55. česko-slovenská psychofarmakologická konference. 04.01.2013–08.01.2013. /POSTER/ 193. MISÍK, J., KRAČMAROVÁ, A., KASSA, J. Effect of cholinesterase inhibitors on cholinergic deficit induced by 3-quinuclidinyl benzilate in rats performing the multiple T-maze. Barcelona, Spain: 26th ECNP Congress. 05.10.2013–10.10.2013. /POSTER/ 194. MUSÍLEK, K., KAŠPÁREK, A., HOROVÁ, A., HERMAN, D., KŘENKOVÁ, Z., JUN, D., KUČA, K. Monooxime bisquaternary cholinesterase reactivators with 3-oxapent-1,5-diyl linker – preparation and in vitro screening study. Munich, Germany: 14th Medical Chemical Defence Conference. 24.04.2013–25.04.2013. /POSTER/ 195. MUSÍLEK, K., KAŠPÁREK, A., KUNEŠ, M., HOROVÁ, A., HERMAN, D., KŘENKOVÁ, Z., JUN, D., KUČA, K. Oxime acetylcholinesterase reactivators with 3-oxapent-1,5-diyl linker – synthesis and in vitro evaluation. Tatranské Matliare, Slovakia: 65. Zjazd chemikov. 09.09.2013–13.09.2013. /POSTER/ 196. MUSÍLEK, K., KUNEŠ, M., JUN, D., ŽĎÁROVÁ KARASOVÁ, J., ŠEPSOVÁ, V., KORÁBEČNÝ, J., HRABINOVÁ, M., KUČA, K. Cholinesterase inhibitors as prophylaxis against nerve agents. Velké Karlovice: Syntéza a analýza léčiv. 02.09.2013–05.09.2013. /POSTER/

197. ONDRÁČEK, K., OSIČKOVÁ, J., KRÁL, J., SKOCHOVÁ, H., KOVÁČOVÁ, V., POHANKA, M., BANĎOUCHOVÁ, H., HILSCHEROVÁ, K., MELCÁKOVÁ, V., PIKULA, J. Risk of combined exposure by cyanotoxins, acetylcholinesterase inhibitors and anticoagulants to Xenopus laevis embryos. Hradec Králové: 18th Interdisciplinary

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Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013– 21.06.2013. /POSTER/ 198. OSIČKOVÁ, J., KOVÁČOVÁ, V., BANĎOUCHOVÁ, H., KRÁL, J., SKOCHOVÁ, H., POHANKA, M., NOVOTNÝ, L. Oxidative stress in birds: Effects of heavy metals and pharmaceuticals. Interlaken, Switzerland: 49th Congress of the European-Societies of Toxicology, EUROTOX. 01.08.2013–04.08.2013. /POSTER/ 199. PAJER, P., DRESLER, J., KABÍČKOVÁ, H., KUŽELKA, V., VELEMÍNSKÝ, P., KLIMENTOVÁ, J., STULÍK, J., PEJCHAL, J., ELLEDER, D., BENEŠ, V., MEYER, H., DUNDR, P., HUBÁLEK, M., PÍŠA, L. Variola virus in historical samples from the National Museum of Prague. Munich, Germany: Medical Biodefense Conference 2013. 22.10.2013–25.10.2013. /POSTER/ 200. PÁRAL, J., LOCHMAN, P., ŠIMEK, J. MASCAL. Praha: Mezinárodní kongres AWHP. 17.09.2013–17.09.2013. 201. PÁRAL, J. The role of military surgery in the current system of medical services of the Army of Czech Republic. Jívová: Odborné zaměstnání Hlavního lékaře SpS. 23.05.2013–24.05.2013. 202. PÁRAL, J., LOCHMAN, P., PAVLÍK, M. Glue versus stapled anastomosis of the colon; comparison of resistance to intraluminal pressure: an experimental study. Tel Aviv, Israel: 4th World Congress for the Advancement of Surgery. 28.10.2013–30.10.2013. /POSTER/ 203. PÁRAL, J., PLODR, M. Czech field surgical team in Afghanistan. Olomouc: 8. Olomoucké dny urgentní medicíny. 15.05.2013– 17.05.2013. 204. PÁVKOVÁ, I. Comparative proteome analysis of Francisella tularensis after interaction with host cell. Hradec Králové: 3. neformální proteomické setkání. 21.11.2013–22.11.2013. 205. PAVLÍK, V., HLÚBIK, P., FAJFROVÁ, J., NAVRÁTIL, P. Current preventive program in the Army of the Czech Republic. Nový Smokovec, Slovakia: 21. vedecko-odborná konferencia s medzinárodnou účasťou Životné podmienky a zdravie. 23.09.2013–25.09.2013. 206. PAVLÍK, V., HLÚBIK, P., FAJFROVÁ, J. Prevention of obezity in the army of the Czech Republic. Teplice: Výživa a zdraví. 18.09.2013–19.09.2013. /POSTER/ 207. PAVLÍK, V., HLÚBIK, P., FAJFROVÁ, J. Primary and secondary prevention on obesity in the Army of the CR. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/

22th Annual Report, 2013 – 151 PRESENTATIONS AND POSTERS

208. PAVLIŠ, O., POHANKA, M., SOBOTKA, J. Application of melatonin to mice infected by tularemia. Kutná hora: 16. konference o labora- torních zvířatech. 15.05.2013–17.05.2013. 209. PETRÁNEK, K., VANĚK, J., JEŽEK, B., JANEČKA, P. Robust image comparison using mutual information. Horní Lomná: 33. konference geometrii a grafice. 09.09.2013–12.09.2013. 210. PLZÁKOVÁ, L. My theme of thesis. Velká Úpa: Výroční zasedání ÚMP a CPS za rok 2013. 28.11.2013–29.11.2013. 211. POHANKA, M. Cholinesterases and their application in biosensors construction. Brno: Excelence doktorského studia na AF Mendelu pro navazující evropskou vědecko-výzkumnou kariéru. 18.10.2013– 18.10.2013. 212. POHANKA, M., ADAM, V., KZEK, R. Electrochemical assay of butyrylcholinesterase in biological samples. Brno: 13. Pracovní setkání fyzikálních chemiků a elektrochemiků. 29.05.2013–30.05.2013. 213. POHANKA, M. Modulation of immunization via cholinergic nervous system using HI-6. Baltimore, USA: 6th International Congress on Bacteriology and Infection Diseases. 20.11.2013–22.11.2013. 214. POHANKA, M., ŠTĚTINA, R., SVOBODOVÁ, H., RUTTKAY_NEDECKÝ, B., JÍLKOVÁ, M., SOCHOR, J., SOBOTKA, J., ADAM, V., KIZEK, R. Sulfur mustard causes oxidative stress and depletion of antioxidants in organs of wistar rats. Brno: 37. Brněnské onkologické dny a 27. Konference pro nelékařské zdravotnické pracovníky. 18.04.2013–19.04.2013. /POSTER/ 215. POHANKA, M. Modulation of immunization via cholinergic nervous system using HI-6. Washington, USA: 62nd ASTMH. 13.11.2013– 17.11.2013. /POSTER/ 216. POHANKA, M. Cholinesterases and their use for assay of poisons. 1. Lékařská fakulta Univerzity Karlovy, Praha: Výroční zasedání institutu Danone. 02.12.2013–02.12.2013. 217. PRAVDOVÁ, L., JAKL, M. The failure of antiplatelet therapy. Hradec Králové: 8. fakultní vědecká konference studentské tvůrčí činnosti. 24.09.2013–24.09.2013. 218. PROCHÁZKA, M., AGH, K. Active shooter – Metro 2013. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013.

22th Annual Report, 2013 – 152 PRESENTATIONS AND POSTERS

219. PROCHÁZKA, M., HALAJČUK, T. Trauma planning of health care providers. Ostrava: Seminář Krajského úřadu Moravskoslezského kraje. 24.01.2013–24.01.2013. 220. PROCHÁZKA, M., FIŠER, V. Trauma planning of the inpatient and one day health care providers. Hradec Králové: Seminář Centra andragogiky. 17.01.2013–17.01.2013. 221. PROCHÁZKA, M., JEŽEK, T. 10 years of the Disaster Medicine Conference in Hradec Králové. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013. 222. PROCHÁZKA, M., HALAJČUK, T. Financing of the pre-hospital emergency care in the Czech Republic. Sunny Beach, Bulgaria: International. Scientific Symposium Economy & Business. 01.09.2013– 06.09.2013. 223. PROCHÁZKA, M., FIŠER, V. Trauma planning – recent reality and Czech legislation terminology. Hradec Králové: Seminář Centra andragogiky. 17.01.2013–17.01.2013. 224. PRYMULA, R., CHLÍBEK, R. Pneumococcal disease and new recommendation for adult immunization in the Czech Republic. Munich, Germany: 2nd International Conference on Controversies in Vaccination in Adults (CoVAC). 14.02.2013–17.02.2013. 225. PRYMULA, R. New vaccine for the prevention of infectious diseases. Poprad, Slovakia: 17. slovensko-český kongres o infekčných chorobách. 13.06.2013–15.06.2013. 226. PRYMULA, R. Two-dose toddler vaccination with investigational meningococcal B recombinant vaccine – antibody persistence and response to booster at 24 months. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. 227. PRYMULA, R. Antibody persistence one year after vaccination with four doses of the investigationa meningococcal serogroup B vaccine, 4CMENB. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013– 01.06.2013. 228. PRYMULA, R. Measles: whan can we learn about recent outbreaks? Praha: 18th Central European Vaccination Awareness Group Meeting. 24.03.2013–25.03.2013. 229. PRYMULA, R. European studies: A3L39/40. Lyon, France: Hexacima Masterclass. 25.09.2013–26.09.2013.

22th Annual Report, 2013 – 153 PRESENTATIONS AND POSTERS

230. PRYMULA, R. Communication through new media: Is there a place for pro-vaccine messages? Experience of VACCINEWSNET. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. 231. PRYMULA, R. Communication through new media: Is there a place for pro-vaccine messages? Experience of VACCINEWSNET. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. /POSTER/ 232. PRYMULA, R. Current European recommendation for use of PCV in adults. Krakow, Poland: Pneumo Surveillance Workshop. 16.04.2013–18.04.2013. 233. PRYMULA, R. News in vaccination of children and views the introduction of new vaccines. Štrbské Pleso; Slovakia: 4. Slovenský vakcinologický kongres. 17.01.2013–19.01.2013. 234. PRYMULA, R. PCV in prevention of pneumococcal diseases. Skopje, Macedonia: Pediatric Conference. 15.03.2013–16.03.2013. 235. PRYMULA, R. Development of the vaccination calendar in Europe. Skopje, Macedonia: Pediatric Conference. 15.03.2013–16.03.2013. 236. PRYMULA, R. Two-dose toddler vaccination with investigational meningococcal B recombinant vaccine – antibody persistence and response to booster at 24 months. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. /POSTER/ 237. PSUTKA, J., MALÝ, V., HUMLÍČEK, V., MRVEČKA, P. MEDEVAC 2011–2013. Hradec Králové: 10. ročník celostátní konference Medicína katastrof. Zkušenosti, příprava, praxe. 28.11.2013–29.11.2013. /POSTER/ 238. PUTZOVÁ, D. Fast protein liquid chromatography. Hradec Králové: Interní výukový seminář laboratorních metod. 26.02.2013–26.02.2013. 239. RUTTKAY-NEDECKÝ, B., HYNEK, D., MAŠKOVÁ, D., NEJDL, L., POHANKA, M., ADAM, V., KIZEK, R. Use of electrochemical methods for evaluation of critical health condition. Brno: 8. Pracovní setkání fyzikálních chemiků a elektrochemiků. 29.05.2013–30.05.2013. /POSTER/ 240. SHESHKO, V. Expression of recombinant proteins. Hradec Králové: VÝUKOVÝ SEMINÁŘ LABORATORNÍCH METOD. 12.02.2013– 12.02.2013. 241. SHESHKO, V., PUTZOVÁ, D., STULÍK, J. Identification, cloning, expression, and purification of Francisella tularensis immunogenic membrane proteins. Urgup, Turkey: International symposium

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on Francisella tularensis and Tularemia. 18.06.2013–23.06.2013. /POSTER/ 242. SHESHKO, V., PUTZOVÁ, D., STULÍK, J. Identification, cloning, expression, and purification of Francisella tularensis immunogenic proteins. Munich, Germany: Medical Biodefense Conference 2013. 22.10.2013–25.10.2013. /POSTER/ 243. SCHMIDT, M. DsbA – never ending story. Velká Úpa: Výroční zasedání UMP a CPS. 28.11.2013–29.11.2013. 244. SCHMIDT, M., STULÍK, J., PÁVKOVÁ, I. The role of cis-proline residue in DsbA homologue of Francisella tularensis subsp. holarctica FSC200. Urgup, Turkey: International Symposium on Francisella Tularensis and Tularemia. 19.06.2013–22.06.2013. /POSTER/ 245. SLANINKA, I., KLEIN, L., ČÁP, R., HOŠEK, F., HASENÖHRLOVÁ, L. Microstoma of oral cavity after chemical burns by alkali solution – a case report. Nitra, Slovakia: Medzinárodný kongres plastickej chirurgie a 10. Demjínov deň. 16.05.2013–17.05.2013. 246. SMETANA, J. Everything you wanted to know about vaccination and did not have time to ask. Doksy: Mezikrajský seminář epidemiologů. 04.06.2013–06.06.2013. 247. SMETANA, J., CHLÍBEK, R., BOŠTÍKOVÁ, V. Present vaccination against tetanus. Hradec Králové: 9. Hradecké vakcinologické dny. 03.10.2013–05.10.2013. 248. SMETANA, J., KOSINA, P. Vaccination in questions and answers – parent view. Praha: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 23.10.2013–23.10.2013. 249. SMETANA, J. Agent medical countermeasures. Vyškov: JCBRN Defence Centre of Excellence – Crisis Management after CBRN Incident/Pilot Course. 11.11.2013–15.11.2013. 250. SMETANA, J., KOSINA, P. Questions and answers on the practice pediatrician. Plzeň: Vzdělávací seminář pro PLDD na téma Očkování. 10.09.2013–10.09.2013. 251. SMETANA, J. Vaccinations recommended for the adult population. Ostrava: Cyklus Vzdělávání regionálních center. 04.06.2013– 04.06.2013. 252. SMETANA, J., KOSINA, P. Vaccination in questions and answers – parent view. Jihlava: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 25.09.2013– 25.09.2013.

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253. SMETANA, J., KOSINA, P. Questions and answers on the practice pediatrician. Praha: Celorepublikový cyklus vzdělávacích seminárů pro praktické lékaře pro děti a dorost na téma Očkování. 15.06.2013– 15.06.2013. 254. SMETANA, J. News vaccination in adults. Praha: Školení lékařů spádových zdravotnických zařízení. 27.03.2013–27.03.2013. 255. SMETANA, J., KOSINA, P. Vaccination in questions and answers – parent view. Zlín: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 13.11.2013–13.11.2013. 256. SMETANA, J., CHLÍBEK, R. Optional vaccination from a vaccinologist´s point of view. Praha: Celorepublikový cyklus vzdělávacích seminárů pro praktické lékaře pro děti a dorost na téma Očkování. 15.06.2013– 15.06.2013. 257. SMETANA, J., CHLÍBEK, R., BOŠTÍKOVÁ, V. Vaccination against tetanus in adulthood, new recommendations. Hradec Králové: 9. Hradecké vakcinologické dny – Sympozium všeobecných praktických lékařů. 03.10.2013–05.10.2013. 258. SMETANA, J., KOSINA, P. Vaccination in questions and answers – parent view. Hradec Králové: Vzdělávací seminář SPLDD na téma 2+1 netradiční pohledy na očkování a péči o pokožku. 06.11.2013– 06.11.2013.

259. SNAPE, M., FINN, A., HEATH, P., BONA, G., ESPOSITO, S., PRINCIPI, N., DIEZ-DOMINGO, J., SOKAL, E., BIRGITTA, B., KIENINGER, D., PRYMULA, R., WANG, H., TONEATTO, D., DULL, P., POLLARD, A. Persistence to 12, 18 and 24 months of bactericidal antibodies induces by infant immunisation with a serogroup B meningococcal vaccine. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. /POSTER/ 260. SNAPE, M., FINN, A., HEATH, P., BONA, G., ESPOSITO, S., PRINCIPI, N., DIEZ-DOMINGO, J., SOKAL, E., BIRGITTA, B., KIENINGER, D., PRYMULA, R., WANG, H., TONEATTO, D., DULL, P., POLLARD, A. Persistence to 12, 18 and 24 months of bactericidal antibodies induces by infant immunisation with a serogroup B meningococcal vaccine. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. 261. SOUKUP, O., KORÁBEČNÝ, J., ŠPILOVSKÁ, K., MUSÍLEK, K., JUN, D., MISÍK, J., ŽĎÁROVÁ KARASOVÁ, J., KUČA, K. Hybrids of AChE inhibitor and memantine derivatives as candidates for Alzheimer´s disease treatment. San Diego, USA: Neuroscience 2013. 09.11.2013– 13.11.2013. /POSTER/

22th Annual Report, 2013 – 156 PRESENTATIONS AND POSTERS

262. SOUKUP, O. Acetylcholinesterase. St. Andrews, Great Britain: Acetylcholinesterase Meeting. 07.05.2013. 263. SOUKUP, O., JUN, D., TOBIN, G., KUČA, K. Nom-reactivating properties of oxime reactivators. Changwon, South Korea: Korean Chemical Society Meeting. 16.10.2013–18.10.2013. /POSTER/ 264. SOUKUP, O., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., JUN, D., KUČA, K. Amantadine and AChE inhibitor hybrids as candidates for Alzheimer disease treatment. Boston, USA: Alzheimer's Association International Conference. 13.07.2013–18.07.2013. /POSTER/ 265. STŘÍTECKÁ, H. Dietary fat in prevention of CVD. Praha: Výživové poradenství v praxi – dvodenní seminář AVP Nutris. 29.09.2013– 30.09.2013. 266. STŘÍTECKÁ, H. Nutrition for children. Praha: Vzdělávací seminář Dexter Academy. 07.04.2013–07.04.2013. 267. STŘÍTECKÁ, H. Human nutrition. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 02.03.2013–02.03.2013. 268. STŘÍTECKÁ, H. Nutrition of pregnant and breastfeeding. Praha: Vzdělávací seminář Dexter Academy. 06.04.2013–06.04.2013. 269. STŘÍTECKÁ, H. Fluid intake, quality of beverages in the trade market. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 03.03.2013–03.03.2013. 270. STŘÍTECKÁ, H., ZMRZLÁ, H., MARINOV, Z. Children obesity and overweight at pediatrics ambulations. Hradec Králové: Obezitologie a bariatrie 2013. 17.10.2013–19.10.2013. /POSTER/ 271. STŘÍTECKÁ, H., HLÚBIK, P. Effects to satiation and weight reduction of fermented milk product enriched with protein and fibre. Granada; Spain: IUNS 20th Internationla Congress of Nutrition. 15.09.2013– 20.09.2013. /POSTER/ 272. STŘÍTECKÁ, H. Nutrition as a factor for preventable of civilization diseases. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 02.03.2013–02.03.2013. 273. STŘÍTECKÁ, H. Prevalence of overweight and obesity in the project Caterpillar. Poděbrady: Konference kazuistik a ambulantní práce – praktická dětská obezitologie II. 14.06.2013–15.06.2013. 274. STŘÍTECKÁ, H., HLÚBIK, P. How the Czech Army solves the problem with obesity. Liverpool, Great Britain: ECO 2013. 12.05.2013– 16.05.2013. /POSTER/

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275. STŘÍTECKÁ, H., ZMRZLÁ, H. What children prefer in the diet and why? Poděbrady: Dětská výživa a obezita v teorii a praxi. 06.12.2013– 07.12.2013. 276. STŘÍTECKÁ, H., ZMRZLÁ, H., HLÚBIK, P. Comparison of eating habits at school children with normal and excess weight/obesity. Spain; Granada: IUNS 20th International Congresss of Nutrition. 15.09.2013– 20.09.2013. 277. STŘÍTECKÁ, H. The criteria for selection of healthy foods. Praha: Výživové poradenství v praxi – dvodenní seminář AVP Nutris. 09.03.2013–10.03.2013. 278. STŘÍTECKÁ, H. Workshop – practical dietary advice. Poděbrady: Konference kazuistik a ambulantní práce – praktická dětská obezitologie II. 14.06.2013–15.06.2013. 279. STŘÍTECKÁ, H., HLÚBIK, P., ZMRZLÁ, H. The eating habits of primary school children – comparison of children with normal and excess weight/ obesity. Liverpool, Great Britain: ECO 2013. 12.05.2013–16.05.2013. /POSTER/ 280. STŘÍTECKÁ, H. The nutritional care in the treatment of obesity. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 17.03.2012–17.03.2012. 281. STŘÍTECKÁ, H., ZAMRAZILOVÁ, H., DIVOKÁ, J., TLÁSKAL, P., BOŽENSKÝ, J., HOSTINSKÝ, J. Forum prevention of childhood overweight and obesity (FPDNO). Hradec Králové: Obezitologie a bariatrie 2013. 17.10.2013–19.10.2013. /POSTER/ 282. STŘÍTECKÁ, H., ZMRZLÁ, H., HLÚBIK, P. Is there any differences? – Comparison of eating habits at school children with normal and excess weight/obesity. Granada, Spain: IUNS 20th International Congresss of Nutrition. 15.09.2013–20.09.2013. /POSTER/ 283. STŘÍTECKÁ, H. The possibilities of nutritional counselling in child obesitology. Poděbrady: Konference kazuistik a ambulantní práce – praktická dětská obezitologie II. 14.06.2013–15.06.2013. 284. STŘÍTECKÁ, H. Rules for the design and assessment of eating records. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 09.03.2013–09.03.2013. 285. STŘÍTECKÁ, H. Basics of anatomy of the digestive tract – physiology of digestion and absorption. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 23.02.2013–23.02.2013.

22th Annual Report, 2013 – 158 PRESENTATIONS AND POSTERS

286. STŘÍTECKÁ, H. Basics of biochemistry – metabolism of protein, carbohydrates and fats. Hradec Králové: Akreditovaný rekvalifikační kurz Výživový poradce. 24.02.2013–24.02.2013. 287. STULÍK, J. Quantitative proteomics analysis of macrophage rafts reveals induction of autophagy pathway at the early time of Francisella tularensis LVS infection. Urgup, Turkey: International Symposium on Francisella tularensis and Tularemia. 19.06.2013–22.06.2013. 288. STULÍK, J. Quantitative proteomic identification of macrophage proteins involved in the interaction with Francisella tularensis. Hradec Králové: 3. Neformální proteomické setkání. 21.11.2013–22.11.2013. 289. STULÍK, J. Quantitative proteomic identification of macrophage proteins involved in the interaction with Francisella tularensis. Iraklion, Greece: Working Meeting at Department of Chemistry, University of Crete. 13.11.2013–16.11.2013. 290. STULÍK, J. Scientific work of Institute of Molecular Pathology year 2013. Velká Úpa: Výroční zasedání ÚMP a CPS za rok 2013. 28.11.2013– 29.11.2013. 291. ŠENITKOVÁ, I. In vivo and in vitro testing of FSC200/DSBA-like mutant. Velká Úpa, Krkonoše: Výroční zasedání ÚMP a CPS za rok 2013. 28.11.2013–29.11.2013. 292. ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., DRTINOVÁ, L., KORÁBEČNÝ, J., ŠPILOVSKÁ, K., SOUKUP, O. The influence of acetylcholinesterase inhibitors on cholinergic system of urinary bladder. Lázně Jeseník: 55. česko-slovenská psychofarmakologická konference. 04.01.2013–08.01.2013. /POSTER/ 293. ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., DRTINOVÁ, L., ZEMEK, F., ŠPILOVSKÁ, K., SOUKUP, O. Derivates of 7-meota-donepezil, a new acetylcholinesterase inhibitor, act as modulators of muscarinic receptors. Hangzhou, China: 14th Symposium on Cholinergic Mechanism. 05.05.2013–09.05.2013. /POSTER/ 294. ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., SOUKUP, O., KORÁBEČNÝ, J., ŠPILOVSKÁ, K., MISÍK, J. Tacrine, 7-meota and new acetylcholinesterase inhibitor K344 act as modulators of muscarinic receptors. Florence, Italy: AD/PD™ 2013: The 11th International Conference on Alzheimer’s and Parkinson’s Diseases. 06.03.2013– 10.03.2013. /POSTER/ 295. ŠEPSOVÁ, V. Observation of cholinesterase inhibitor's effects on muscarinic M1 receptors. Brno: 3. ročník mezioborového semináře mladých farmakologů a toxikologů. 23.05.2013–23.05.2013.

22th Annual Report, 2013 – 159 PRESENTATIONS AND POSTERS

296. ŠEPSOVÁ, V., ŽĎÁROVÁ KARASOVÁ, J., SOUKUP, O. Testing of the effects of ACHE inhibitors on the M1 muscarinic receptors. Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference (TOXCON 2013). 19.06.2013–21.06.2013. /POSTER/ 297. ŠINKOROVÁ, Z., ZÁRYBNICKÁ, L., NAVRÁTIL, L., ČASTULÍK, P. A flow cytometric study on DEMRON-mediated radioprotection in mice. San Diego, California, USA: The 27th Congress of the International Society for Advancement of Cytometry. 19.05.2013–22.05.2013. /POSTER/ 298. ŠINKOROVÁ, Z., ZÁRYBNICKÁ, L., ČASTULÍK, P. Evaluation of radioprotective properties of suits made by Demron technology. Žilina, Slovakia: 18. mezinárodná vedecká konferencia Riešenie krízových situácií v špecifickom prostredí. 05.06.2013–06.06.2013. 299. ŠINKOROVÁ, Z., ZÁRYBNICKÁ, L. Radioprotective effect od nanomaterias. Hradec králové: Nové pohledy na účinek ionizujícího záření na živý organismus 2013. 11.12.2013–11.12.2013. 300. ŠMEJKAL, K. Humeral shaft fractures. Plzeň: Bohemian-Bavarian Congress. 21.06.2013–22.06.2013. 301. ŠMEJKAL, K. Fractures of blades. Plzeň: Bohemian-Bavarian Congress. 21.06.2013–22.06.2013. 302. ŠPIDLOVÁ, P. Polymerase chain reaction PCR. Hradec Králové: Interní výukový seminář laboratorních metod. 12.02.2013–12.02.2013. 303. ŠPIDLOVÁ, P., STRAŠKOVÁ, A., BALONOVÁ, L., ŽÁKOVÁ, J., STULÍK, J. Francisella tularensis SchuS4 immunoreactive proteins. Urgup, Turkey: International Symposium on Francisella Tularensis and Tularemia. 19.06.2013–22.06.2013. /POSTER/ 304. ŠPIDLOVÁ, P., ŠENITKOVÁ, I. Protein – protein interactions: new possibilities of study using genetic systems. Velká Úpa: Výroční zasedáná ÚMP a CPS za rok 2013. 28.11.2013–29.11.2013. 305. ŠPIDLOVÁ, P. Site-directed mutagenesis of FTS_0065 and FTS_1067 O – glycosylation sites. Velká Úpa: Výroční zasedání ÚMP a CPS za rok 2013. 28.11.2013–29.11.2013. 306. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., DRTINOVÁ, L., ŠEPSOVÁ, V., KUČA, K. Synthesis and in vitro screening of 7-methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in Alzheimer's disease therapy. Hangzhou, China: 14th International Symposium on Cholinergic Mechanisms. 05.05.2013–09.05.2013. /POSTER/

22th Annual Report, 2013 – 160 PRESENTATIONS AND POSTERS

307. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., BENEK, O., KUČA, K. Synthesis and biological testing of 7- methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in Alzheimer's disease. Lázně Jeseník: 55. česko-slovenská psychofarmakologická konference. 04.01.2013– 08.01.2013. /POSTER/ 308. ŠPILOVSKÁ, K., KORÁBEČNÝ, J., MUSÍLEK, K., HOROVÁ, A., BENEK, O., DRTINOVÁ, L., ŠEPSOVÁ, V., KASSA, J., KUČA, K., NEPOVIMOVÁ, E. 7-methoxytacrine-adamantylamine heterodimers as potential cholinesterase inhibitors in AD treatment – synthesis, biological evaluation and molecular docking. Florence, Italy: AD/PD™ 2013: The 11th International Conference on Alzheimer’s and Parkinson’s Diseases. 06.03.2013–10.03.2013. /POSTER/ 309. ŠPLIŇO, M. Systemic review of influenza. Malaga, Spain: Advisory Board CDC. 08.09.2013–13.09.2013. 310. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Measles – recurring problem in Europe. Hradec Králové: 9. Hradecké vakcinologické dny. 03.10.2013– 05.10.2013. 311. ŠPLIŇO, M., BOŠTÍKOVÁ, V. Hepatitis C Virus – The discovery of homologous virus in dogs – Canine hepacivirus – CHV (2011). Hradec Králové: 18. Hradecké virologické dny. 15.10.2013–16.10.2013. 312. ŠTĚTINA, R., JOŠT, P., JÍLKOVÁ, M., SVOBODOVÁ, H. Mechanism of action of sulphur mustard (SM). Hradec Králové: 18th Interdisciplinary Czech-Slovak Toxicology Conference TOXCON 2013. 19.06.2013– 21.06.2013. 313. ŠTĚTINA, R., HRNČIARIKOVÁ, D., HRNČIARIK, M., FIKROVÁ, P., HRONEK, M., ZADÁK, Z. DNA damage and repair in oncology – metabolic and pharmacologic consequences. Hrade Králové: Společnost klinické výživy a intenzivní metabolické péče ČLS JEP. 07.03.2013–09.03.2013. 314. ŠTĚTINA, R., JOŠT, P., SVOBODOVÁ, H. Different repair of interstrand cross links induced with cisplatin and sulphur mustard (SM). Smolenice, Slovakia: FEBS SNA Repair Workshop. 09.06.2013–13.06.2013. /POSTER/ 315. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Laparoscopic vs. open liver resection for metastatic colorectal cancer: oncological results. Plzeň: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu. 11.09.2013– 13.09.2013.

22th Annual Report, 2013 – 161 PRESENTATIONS AND POSTERS

316. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Postoperative morbidity as a negative prognostic factor for the treatment of liver metastases of colorectal cancer. Bratislava: 67. chirurgický deň Kostlivého. 06.12.2013–06.12.2013. 317. ŠUBRT, Z. First experiences with the generator Caiman in FNHK. Hradec Králové: Techniky hemostázy – elektrochirurgie a lokální hemostatika. 06.06.2013–06.06.2013. 318. ŠUBRT, Z. Casereport of a patient with metastatic colorectal carcinoma. Praha: Jaterní sympozium. 04.11.2013–04.11.2013. 319. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Rare primary tumors – case reports. Plzeň: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu. 11.09.2013–13.09.2013. 320. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Surgical treatment of liver metastases for colorectal cancer: analysis of predictive factors. Plzeň: 40. česko-slovenský chirurgický kongres s mezinárodní účastí a 6. kongres chirurgie jater, žlučových cest a pankreatu. 11.09.2013– 13.09.2013. 321. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Iatrogenic bile duct injury. Ještěd: Pracovní den sekce mladých chirurgů ČCHS. 24.10.2013– 25.10.2013. 322. ŠUBRT, Z., FERKO, A., ČEČKA, F., JON, B. Laparoscopic right hand extended hemihepatektomie for liver metastases of colorectal cancer. Bratislava, Slovakia: 67. chirurgický deň Kostlivého. 06.12.2013– 06.12.2013. 323. ŠUBRT, Z. First experiences with the generator Caiman in FNHK. Hradec Králové: Techniky hemostázy – elektrochirurgie a lokální hemostatika. 22.11.2013–22.11.2013. 324. ŠUBRT, Z. How can hunt appendectomy. Harrachov: 17. Harrachovské chirurgické dny. 13.06.2013–14.06.2013. 325. ŠUBRT, Z. Laparoscopic liver resection for liver metastases of colorectal cancer. Hradec Králové: 14. Bedrnův den. 18.04.2013–19.04.2013. 326. TICHÝ, A., ŠALOVSKÁ, B., FABRIK, I., VÁVROVÁ, J. Comparison of resins for metal oxide affinity chromatography with mass spectrometry detection for the determination of phosphopeptides. Minneapolis, USA: 61st Conference of American Society for Mass Spectrometry. 09.06.2013–13.06.2013. /POSTER/

22th Annual Report, 2013 – 162 PRESENTATIONS AND POSTERS

327. TICHÝ, A., ŠALOVSKÁ, B., FABRIK, I., VÁVROVÁ, J., ŘEZÁČOVÁ, M. Radio-sensitization of human leukemic cells HL-60 by ATR-kinase inhibitor (VE-821). Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 328. TONEATTO, D., PRYMULA, R., MERRALL, E., KOHL, I., DULL, P., VESIKARI, T. Immunogenicity and reactogenicity of two-dose vaccination with investigational meningococcal B recombinant vaccine at 24 and 26 months of age. Milan, Italy: 31st Annual ESPID meeting. 28.05.2013–01.06.2013. /POSTER/ 329. VAJRYCHOVÁ, M. Protein quantification using stable isotope labeling. Hradec Králové: Interní výukový seminář laboratorních metod. 19.02.2013–19.02.2013. 330. VAJRYCHOVÁ, M. Analysis of native peptides in pregnancies complicated by preterm leakage of amniotic fluid. Hradec Králové: 7. fakultní konference studentů doktorských programů. 01.10.2013– 01.10.2013. 331. VAJRYCHOVÁ, M. Analysis of native peptides in pregnancies complicated by preterm leakage of amniotic fluid. Velká Úpa: Výroční zasedání UMP a CPS. 28.11.2013–29.11.2013. 332. VAJRYCHOVÁ, M., TAMBOR, V., KACEROVSKÝ, M., STULÍK, J., LENČO, J. Analysis of amniotic fluid proteome and peptidome in PPROM pregnancies with inflammatory complications. Yokohama, Japan: The 12th Human Proteome Organisation World Congress (12th HUPO). 14.09.2013–18.09.2013. /POSTER/ 333. VANĚK, J., JEŽEK, B. Computer graphics subjects in computer science curricula. Praha: Proceedings of the 10th International Conference Efficiency and Responsibility in Education 2013. 06.06.2013– 07.06.2013. 334. VODIČKA, P., SLYŠKOVÁ, J., ŠTĚTINA, R., VODIČKOVÁ, L., NACCARATI, A., KUMAR, R., HEMMINKI, K. Functional DNA repair tests and their perspectives in molecular epidemiology (experience with subjects exposed to carcinogens and in cancer patients). Courtyard by Marriott Philadelphia Downtown, USA: Cancer Epidemiology, Biomarkers & Prevention, 4th International Conference on Biomarkers & Clinical Research. 15.07.2013–17.07.2013. /POSTER/ 335. VOSEČKOVÁ, A., HRSTKA, Z., PROCHÁZKA, M. Interdiciplinary preparation of specialists for emergency situations. Hradec Králové: 19. královéhradecké ošetřovatelské dny. 12.09.2013–13.09.2013. /POSTER/

22th Annual Report, 2013 – 163 PRESENTATIONS AND POSTERS

336. WITT, P., PSUTKA, J., MALÝ, V. KOT-ZDR Pandur II. Hradec Králové: 13. konference odborné Společnosti vojenských lékařů, farmaceutů a veterinárních lékařů ČLS JEP. 24.10.2013–24.10.2013. /POSTER/ 337. ZAMRAZILOVÁ, H., TLÁSKAL, P., BOŽENSKÝ, J., BŘEZKOVÁ, V., STŘÍTECKÁ, H., DIVOKÁ, J., FLORIÁNKOVÁ, M., HOSTINSKÝ, J., KŘÍSTEK, N., MARÁDOVÁ, E., PACKOVÁ, A. Forum of prevention childhood overweight and obesity – activation of selected moments in education and lifestyle in the school environment. Pardubice: Školní stravování 2013. 14.05.2013–16.05.2013. 338. ZÁRYBNICKÁ, L., ŠINKOROVÁ, Z., VÁVROVÁ, J. The of radio- sensitivity of lymphocytes by surface and intracellular phenotyping. Praha: Odborný seminář průtokové cytometrie. 07.02.2013–08.02.2013. 339. ZÁRYBNICKÁ, L., ŠINKOROVÁ, Z. Flow cytometry. Hradec Králové: Interní výukový seminář laboratorních metod I. 12.02.2013–12.02.2013. 340. ZÁRYBNICKÁ, L. The biodosimetric study. Fontenay aux Roses, France: CEA – Institute of Cellular and Molecular Radiobiology. 29.10.2013–28.11.2013. 341. ZÁRYBNICKÁ, L. Molecular mechanisms of DNA reparation. Hradec Králové: Seminář – Nové pohledy na účinek ionizujícího záření na živý organismus. 11.12.2013–11.12.2013.

22th Annual Report, 2013 – 164 INDEX INDEX

A DRTINOVÁ, L. 29, 104, 109, 111, 115, 125, 130, 133, 146, 150, ANTONINOVÁ, J. 29 159, 160, 161 ANTOŠ, K. 59, 121, 123 DUŠEK, T. 48 B DUŠKOVÁ, K. 52, 57 BAJGAR, J. 14, 65, 88, 114, 117, E 121, 122, 128, 144, 146 ETFLAIŠOVÁ, P. 74 BALONOVÁ, L. 68, 132, 133, 160 BEDNARČÍK, P. 57, 118, 134 F BENDOVÁ, T. 52 FABRIK, I. 68, 104, 111, 133, 134, 162, 163 BERNARDOVÁ, M. 38 FAJFROVÁ, J. 57, 92, 108, 116, BLANAŘ, R. 25, 55, 88 118, 122, 129, 134, 151 BLAŽEK, P. 36 FORETOVÁ, Z. 68 BOCKOVÁ. I. 74 FUČÍKOVÁ, A. 68, 71, 93, 95, BOŠTÍK, P. 3, 16, 25, 26, 29, 31, 133, 135 32, 33, 34, 35, 42, 43, 44, 71, FUSEK, J. 26, 59, 109, 113 72, 75, 76, 77, 88, 98, 99, 100, 101, 103, 110, 114, 115, 119, G 126, 132, 147 GRÖGER, T. 52, 135 BOŠTÍKOVÁ, V. 31, 34, 35, 38, H 42, 43, 44, 72, 79, 80, 88, 98, 100, 101, 103, 110, 114, 115, HALAJČUK, T. 59, 61, 76, 96, 116, 119, 120, 126, 129, 130, 116, 119, 121, 135, 143, 153 132, 140, 142, 155, 156, 161 HANOVCOVÁ, I. 33, 38, 43, 71, BRNDIAR, M. 27, 28, 45, 137 98, 114, 116, 119 C HEJČOVÁ, J. 59 CABAL, J. 65, 107, 108, 129, 149 HERMAN, D. 29, 150 ČÁP, R. 48, 49, 126, 132, 155 HLAVÁČKOVÁ, H. 59 ČMEDLOVÁ, P. 52, 57 HLÚBIK, P. 26, 57, 78, 80, 105, 115, 116, 119, 122, 124, 125, D 129, 134, 136, 151, 157, 158 DOBEŠ, D. 48, 49, 105, 109, 110, HORÁČEK, J. 26, 45, 79, 80, 133 105, 112, 116, 124, 126, 127, DOHNALOVÁ, L., 76 128, 136, 137, 138, 142, 148 DOLEŽELOVÁ, M. 52 HRABINOVÁ, M. 29, 32, 101, 103, 106, 117, 127, 135, 147, DRESLER, J. 15, 68, 94, 133, 148, 150 135, 151

22th Annual Report, 2013 – 165 INDEX

HRDLIČKOVÁ, L. 36 KASSA, J. 13, 26, 32, 60, 65, 66, HRSTKA, Z. 26, 27, 28, 59, 61, 75, 76, 77, 79, 88, 89, 100, 96, 138, 139, 163 102, 106, 124, 130, 133, 144, 145, 146, 148, 149, 150, 161 HUMLÍČEK, V. 55, 116, 122, 123, 139, 154 KLEIN, L. 26, 48, 50, 75, 77, 80, 89, 116, 117, 118, 119, 126, HUSÁROVÁ, M. 57 132, 145, 155 CH KLIMENTOVÁ, J. 68, 110, 133, CHLÍBEK, R. 15, 25, 26, 38, 39, 145, 151 40, 41, 42, 75, 76, 77, 78, 79, KOMÁREK, V. 36 88, 96, 97, 99, 103, 105, 107, KONŠTACKÝ, S. 27, 28, 52, 117 110, 112, 114, 115, 116, 118, 119, 121, 122, 123, 126, 127, KORÁBEČNÝ, J. 16, 65, 80, 92, 128, 129, 130, 132, 133, 139, 106, 111, 113, 117, 120, 130, 140, 141, 142, 149, 153, 155, 132, 133, 144, 145, 147, 150, 156 156, 157, 159, 160, 161 KOŠŤÁLOVÁ, L. 38 J KRAČMAROVÁ, A. 29, 65, 117, JAKL, M. 26, 45, 105, 124, 126, 124, 128, 133, 146, 150 137, 138, 142, 152 KROČOVÁ, Z. 16, 33, 34, 43, 68, JANOUCH, J. 59 71, 72, 77, 78, 89, 92, 98, 99, JAREŠOVÁ, L. 59, 142, 143 100, 122, 135, 146, 147 JEBAVÝ, L. 26, 45, 47, 80, 88, KRUTIŠ, J. 52 96, 105, 116, 119, 124, 126, KRUTIŠOVÁ, P. 52 127, 128, 136, 137, 138, 143, 148 KUBELKOVÁ, K. 26, 29, 76, 77, 78, 122, 146, 147 JEŽEK, B. 59, 61, 96, 116, 119, 121, 122, 123, 135, 142, 143, KUČA, K. 16, 26, 29, 32, 33, 34, 144, 152, 153, 163 60, 61, 66, 77, 80, 89, 95, 99, 100, 101, 103, 106, 107, 108, JOŠT, P. 29, 65, 108, 144, 161 111, 112, 113, 115, 117, 118, JUN, D. 26, 29, 33, 34, 43, 61, 120, 127, 128, 129, 130, 131, 71, 95, 98, 103, 106, 111, 113, 132, 133, 135, 136, 142, 144, 115, 117, 120, 127, 128, 130, 145, 147, 148, 149, 150, 156, 131, 135, 144, 145, 147, 148, 157, 160, 161 149, 150, 156, 157 KULICH, M. 59 JUNKOVÁ, I. 74 KUPSA, T. 45, 79, 80, 105, 116, K 124, 126, 127, 128, 136, 137, 138, 148 KAČEROVSKÝ, J. 45, 105, 111, 143, 149, 163 L KAISLEROVÁ, L. 29, 35, 44, 99, LAŠÁK, P. 59 114, 115 LEGERSKÁ, K. 38

22th Annual Report, 2013 – 166 INDEX

LENČO, J. 68, 70, 95, 104, 105, PAVLIŠ, O. 65, 93, 109, 110, 118, 111, 135, 148, 149, 163 146, 152 LINK, M. 68, 133, 138, 149 PEJCHAL, J. 29, 31, 34, 43, 65, LOCHMAN, P. 26, 48, 49, 77, 72, 96, 100, 103, 106, 108, 129, 151 112, 122, 147, 151 LUKŠÍKOVÁ, L. 68 PEREGRINOVÁ, J. 57 PÍRKOVÁ, J. 57 M POHANKA, M. 27, 28, 29, 32, 34, MACELA, A. 15, 33, 43, 68, 71, 61, 79, 80, 89, 104, 105, 106, 98, 108, 122, 135, 146, 147 108, 109, 110, 111, 112, 115, MACHAČOVÁ, I. 45 118, 120, 122, 124, 125, 129, MALÍKOVÁ, M. 38 133, 146, 150, 151, 152, 154 MALÝ, V. 55, 105, 119, 123, 124, POTÁČ, M. 27, 28, 55 126, 137, 142, 154, 164 PROCHÁZKA, M. 25, 27, 28, 59, MAREK, J. 38, 129, 149 61, 76, 95, 116, 119, 121, 123, 135, 143, 152, 153, 163 MATOUŠEK, R. 52, 149 PRŮCHOVÁ, Š. 62 MAZUROVÁ, I. 29 PRYMULA, R. 11, 12, 14, 15, 26, MERVARTOVÁ, L. 62 38, 40, 41, 90, 99, 107, 112, MISÍK, J. 32, 60, 65, 66, 77, 79, 119, 122, 123, 129, 153, 154, 80, 93, 100, 106, 107, 108, 156, 163 118, 124, 129, 130, 133, 144, PSUTKA, J. 13, 26, 55, 123, 154, 145, 146, 149, 150, 156, 159 164 MÍŠKOVÁ, L. 55 PUDÍK, P. 27, 28 MÍŠKOVÁ, Z. 27 PUDÍKOVÁ, M. 74 MRVEČKA, P. 55, 122, 123, 154 R MUSÍLEK, K. 26, 32, 60, 65, 66, 76, 77, 80, 100, 103, 106, 111, RESLOVÁ. E. 65 112, 113, 117, 128, 130, 132, ROHLENA, M. 27, 28 136, 142, 144, 145, 147, 148, RŮŽIČKA, M. 55, 116, 119 149, 150, 156, 157, 160, 161 RYDRYCH, J. 36 P Ř PÁRAL, J. 8, 17, 24, 25, 26, 48, 49, 50, 77, 95, 102, 129, 151 ŘEHULKA, P. 34, 43, 68, 72, 100, 104, 107, 110, 111, 133 PÁVKOVÁ, I. 68, 108, 110, 151, 155 S PAVLÍK, M. 32, 74, 101 SHESHKO, V. 29, 154, 155 PAVLÍK, V. 26, 57, 65, 103, 108, SCHMIDT, M. 29, 68, 108, 110, 111, 113, 116, 118, 122, 129, 135, 155 131, 134, 148, 149, 151 SLAVÍK, J. 74

22th Annual Report, 2013 – 167 INDEX

SMETANA, J. 15, 26, 27, 28, 35, ŠPILOVSKÁ, K. 65, 111, 130, 38, 40, 41, 42, 44, 77, 79, 90, 132, 133, 135, 145, 146, 156, 97, 99, 103, 110, 114, 115, 159, 160, 161 116, 117, 119, 126, 128, 129, ŠPLIŇO, M. 26, 38, 79, 90, 103, 130, 132, 133, 140, 141, 142, 110, 115, 116, 119, 120, 130, 145, 146, 155, 156 140, 142, 161 SOLÁROVÁ, A. 38 ŠTĚTINA, R. 27, 28, 65, 78, 90, SOUKUP, O. 59, 80, 104, 106, 104, 109, 129, 144, 152, 161, 111, 117, 118, 129, 130, 145, 163 147, 148, 149, 156, 157, 159, ŠUBRT, Z. 15, 26, 27, 28, 48, 51, 160 78, 96, 103, 115, 120, 126, STŘÍTECKÁ, H. 27, 28, 57, 79, 130, 131, 134, 161, 162 80, 90, 105, 116, 119, 120, T 124, 125, 133, 136, 157, 158, 159, 164 TICHÝ, A. 27, 28, 45, 62, 63, 92, STULÍK, J. 14, 26, 27, 28, 68, 70, 98, 100, 105, 108, 111, 112, 71, 76, 79, 90, 95, 96, 101, 122, 126, 136, 137, 162, 163 103, 104, 108, 110, 133, 135, TOMÁŠIK, M. 25 138, 151, 154, 155, 159, 160, TÓTHOVÁ, I. 29 163 U SVOBODOVÁ, H. 65, 78, 109, 129, 152, 161 UHLÍŘOVÁ, J. 65 VAJRYCHOVÁ, M. 68, 75, 77, Š 105, 149, 163 ŠAFÁŘOVÁ, M. 68 VANĚK, J. 59, 61, 96, 116, 119, ŠEPSOVÁ, V. 65, 76, 77, 79, 121, 122, 123, 135, 142, 143, 106, 111, 112, 113, 120, 125, 144, 152, 163 130, 133, 144, 145, 146, 147, VÁVROVÁ, J. 26, 62, 103, 104, 150, 159, 160, 161 107, 108, 111, 112, 122, 162, ŠIMEK, J. 48, 151 163, 164 ŠINKOROVÁ, Z. 26, 62, 78, 80, VÍCHOVÁ, E. 36 107, 112, 122, 146, 147, 160, VOPLATKOVÁ, Z. 27, 28, 36 164 VOŽENÍLKOVÁ, K. 38 ŠÍSTKOVÁ, J. 52 ŠKRANCOVÁ, V. 65 W ŠMEJKAL, K. 15, 48, 79, 145, WITT, P. 55, 119, 164 160 Z ŠMÍRA, P. 27, 28 ZAHRADNÍČKOVÁ, J. 48 ŠOŠOVIČKOVÁ, R. 38, 140 ZÁRYBNICKÁ, L. 27, 62, 76, 79, ŠPIDLOVÁ, P. 68, 79, 110, 160 112, 122, 146, 147, 160, 164 ZEDNÍČEK, J. 36 ZIKOVÁ, J. 36

22th Annual Report, 2013 – 168 INDEX

Ž 95, 100, 106, 111, 112, 113, ŽÁKOVÁ, J. 29, 160 117, 120, 125, 128, 130, 131, 144, 145, 146, 147, 148, 150, ŽĎÁROVÁ KARASOVÁ, J. 27, 156, 159, 160 32, 34, 59, 60, 61, 66, 75, 77,

22th Annual Report, 2013 – 169

2013 Annual Report was edited and published by the Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic

Editor: Mgr. Hana Hlaváčková Computer work: Lenka Hrdličková, Mgr. Hana Hlaváčková (using the OBD 3 and the VERSO programs)

Printing: 200 Printed: University of Defence

Contact address: Fakulta vojenského zdravotnictví Třebešská 1575 CZ-500 01 Hradec Králové Tel.: +420 973 253 001 Fax: +420 495 513 018 E-mail: [email protected] E-mail: [email protected]

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ISBN 978-80-7231-355-6