in -resistant baumannii and D. Themelidis1, T. Konstantinidis2, T. Parasidis2, A. Ikonomidis1, M. Panopoulou1

1. Microbiology Laboratory, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece

2. Regional Public Health Laboratory (East Macedonia-Thrace) of Hellenic Centre for Disease Control & Prevention, Alexandroupolis, e P754 Greece Results Of the 251 AB strains studied, 191 (76,1%) were found to be carbapenem-resistant. CR- AB Objectives isolates were also resistant to 3rd generation cephalosporins (100%), ciprofloxacin (100%) and amikacin (50/191-26.2%). TIG resistance was detected in 18 isolates (9.4%) of the CR-AB. The Antimicrobial rates in Greece are among the highest in Europe. The number and the resistance rate of the KP isolates to was 58/614 prevalence of carbapenem-resistant Gram-negative species has increased considerably, (9.44%).21(36,2%) of the 58 CR isolates were also resistant to TIG. Moreover, 6 carbapenem with Klebsiella pneumoniae carbapenemase-producing and carbapenem-resistant sensitive isolates were found to be resistant to TIG. Finally, we found that prevalence of TIG Acinetobacter baumannii are the most challenging multidrug-resistant organisms in resistant strains of KP in our hospital were 64/614 (10,4%) Greece. Few antimicrobials exist to treat carbapenem-resistant Gram-negative infections and Tigecycline (TIG) may be an alternative to the treatment of infection. 120 The aim of the study was to analyze antimicrobial susceptibility of CR Acinetobacter 100 baumannii (AB) and Klebsiella pneumoniae (KP) το tigecycline Table 1: Antimicrobial susceptibility (%) 80 of Acinetobacter baumannii (AB) and 60 Klebsiella pneumoniae (KP) 40 A. baumannii Methods 20 K. pneumniae During a two year period (09/2010−10/2012) we examined non duplicated samples 0 positive for A.baumannii n=251 and Klepsiella pneumonia n=614 corresponding to patients hospitalized in different departments of our hospital. The strains of AB and KP were isolated from follow specimen: blood (61 vs 51), bronchoalveolar excretions (51 Conclusions vs 33) and wound (35 vs 43), catheters (32 vs 27), pus (18 vs 35), urine (18 vs 172), The results of this study have demonstrated that the susceptibility of AB and KP to TIG was high. sputum (11 vs 51), CSF (4 vs 8) and other (21 vs 194). The isolates identification was Treatment options that will help to reduce the symptoms of infections due to carbapenem performed using the automated system VITEK2 (BioMerieux, France). Carbapenem resistant organisms are limited and hence TIG non- susceptibility, MIC >4mcg/ml for or was determined may be considered. The properties of TIG such as stability, safety, low toxicity, non cross- according to CLSI guideline. The MIC values of tigecycline were determined by using the resistance with other and its efficacy against multi-drug resistant A. baumannii and K. E-test system following the manufacturer’s instructions (ABI Biodisk, Sweden), and pneumonia isolates make it our mainstream selection. Nevertheless, monitoring of susceptibility interpreted according to the CLSI guidelines. to TIG is also needed.