CHN2 Antibody Cat. No.: 42-020
CHN2 Antibody
Specifications
HOST SPECIES: Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
IMMUNOGEN: The immunogen for this antibody is: C-QILIENEDVLF
TESTED APPLICATIONS: ELISA, IHC
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate APPLICATIONS: any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6ug/ml.
This antibody is expected to recognize both reported isoforms (NP_004058.1; SPECIFICITY: NP_001035025.1).
Properties
Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.
CLONALITY: Polyclonal
October 2, 2021 1 https://www.prosci-inc.com/chn2-antibody-42-020.html CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION: 500 ug/mL
STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.
Additional Info
OFFICIAL SYMBOL: CHN2
CHN2, chimerin (chimaerin) 2, ARHGAP3, RHOGAP3, RhoGAP3, beta chimerin, Chimerin 2 ALTERNATE NAMES: (GTPase-activating protein, rho, 3), BCH, MGC138360, rho-GTPase-activating protein 3
ACCESSION NO.: NP_001035025.1, NP_004058.1
PROTEIN GI NO.: 91106928
GENE ID: 1124
Background and References
1) Siliceo, M. and Merida, I. T cell receptor-dependent tyrosine phosphorylation of beta2- REFERENCES: chimaerin modulates its Rac-GAP function in T cells. J. Biol. Chem. 284 (17), 11354-11363 (2009).
ANTIBODIES FOR RESEARCH USE ONLY.
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October 2, 2021 2 https://www.prosci-inc.com/chn2-antibody-42-020.html