Anti-TWF1 / PTK9 Antibody (ARG57469)

Product datasheet [email protected]

ARG57469 Package: 100 μl anti-TWF1 / PTK9 antibody Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody recognizes TWF1 / PTK9

Tested Reactivity Hu, Ms, Rat

Tested Application FACS, ICC/IF, IHC-P, WB

Host Mouse

Clonality Monoclonal

Isotype IgG1

Target Name TWF1 / PTK9

Antigen Species Human

Immunogen Purified recombinant fragment of Human TWF1 (aa. 335-384 ) expressed in E. coli.

Conjugation Un-conjugated

Alternate Names PTK9; Protein A6; Twinfilin-1; Protein tyrosine kinase 9; A6

Application Instructions

Application table Application Dilution

FACS 1:200 - 1:400

ICC/IF 1:100

IHC-P 1:200 - 1:1000

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 40 kDa

Properties

Form Liquid

Purification Purified

Buffer PBS and 0.05% Sodium azide.

Preservative 0.05% Sodium azide

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use. www.arigobio.com 1/3 Bioinformation

Gene Symbol TWF1

Gene Full Name twinfilin actin binding protein 1

Background This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

Function Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity). [UniProt]

Cellular Localization Cytoplasm > Cytoskeleton.

Images

ARG57469 anti-TWF1 / PTK9 antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG57469 anti-TWF1 / PTK9 antibody (green). DRAQ5 fluorescent DNA dye (blue).

ARG57469 anti-TWF1 / PTK9 antibody FACS image

Flow Cytometry: HeLa cells stained with ARG57469 anti-TWF1 / PTK9 antibody (right histogram) and negative control (left histogram).

www.arigobio.com 2/3 ARG57469 anti-TWF1 / PTK9 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human bladder cancer tissue stained with ARG57469 anti-TWF1 / PTK9 antibody.

ARG57469 anti-TWF1 / PTK9 antibody WB image

Western blot: HeLa cell lysate stained with ARG57469 anti-TWF1 / PTK9 antibody at 1:5000 dilution.