BIOREACTORS IN BIOTECH: STRATEGIES FOR SELECTION AND OPTIMIZATION 30 November - 10 December 2020 LIVE Online Academy Bioreactors, what are they and how do they work?
Bioreactors are the pillars of bioprocessing, supporting the production of the biomass and the therapeutic product. It is vital that those responsible for operating bioreactors understand the intricacies of their functionality to ensure they can optimize their performance and maximize the bioprocess efficiency.
Over two weeks, this course will explore functionality, including the inoculum expansion process and designing control loops, as well as consider strategies to select the best bioreactor for your product.
In an interactive environment, you will also learn best COURSE practices for avoiding contamination, and characterise your raw materials to ensure a sterile operation OVERVIEW throughout. Gain real time access to a subject matter expert delivering online training in a structured virtual classroom environment.
What is a Live Online Course?
Live online courses are a new interactive and engaging education tool designed to provide training in real time with access to a subject matter expert (trainer). By using webinar technology, a virtual classroom is created providing direct contact with the trainer so you can ask questions, clarify complicated theories and fully understand the topic area.
A live online course is broken down into manageable sessions that are delivered online at set times, providing a LIVE structured learning pathway for delegates. All sessions are recorded and uploaded to our virtual learning environment where you will have unlimited access to the ONLINE materials for a month. COURSES What are the benefits of studying a live online course?
Engagement & interaction All learning content is delivered in real time with our expert faculty, enabling you to have direct contact with the trainer during each session via live Q&A.
Structure & convenience Bitesize sessions are delivered at set times meaning there is minimal disruption on your day to day function.
Cost-effectiveness & accessibility No travel or accommodation costs required for your team to attend training. The course can be studied from any location, all that you need is an internet connection.
Speed & flexibility LIVE Live online courses are fast to organise and deliver meaning your team cam be upskilled quickly. Revisit materials as often ONLINE as you like for a month after the course, as everything is recorded and hosted on our virtual learning environment. COURSES Susan Sharfstein
Susan is currently a professor of Nanobioscience at Suny Polytechnic Institute in New York and has a particular expertise in mammalian cell biotechnology, bioprocessing MEET and chemical engineering. Her research interests involve understanding the effect of culture conditions on living systems used for industrial production of therapeutic THE proteins and carbohydrates. TRAINER SESSION 1 AGENDA - 2PM GMT, 30TH NOVEMBER
Overview of Bioreactors within biotechnology Introduction to the course Overview of bioreactor use in biotechnology - White biotechnology-industrial products - Red biotechnology-medicinal products (main focus of course) - Green-biotechnology- food and agriculture-based products
Overview of modern molecular biology Recombinant DNA technology, gene sequencing, PCR, CRISPR
Introduction to biotherapeutics Antibiotics, therapeutic proteins, monoclonal antibodies, vaccines, heparin, gene therapy, cell therapies, nucleic acid therapeutics SESSION 2 AGENDA - 2PM GMT, 2ND DECEMBER
Biotransformations and host cell organisms Enzymatic transformations, bacteria for recombinant protein production, metabolic engineering of bacteria for the production of small molecules, yeast and other fungi, mammalian cells, insect cells Culture media and feeds
Case study/project 1-evaluate the different potential COVID-19 vaccine types, identify how they would be manufactured SESSION 3 AGENDA - 2PM GMT, 4TH DECEMBER
Engineering considerations in bioprocessing
Kinetics, thermodynamics and transport phenomena (heat and mass transfer, mixing, shear) SESSION 4 AGENDA - 2PM GMT, 7TH DECEMBER
Bioreactor components and types Stirred tank bioreactors Impellor design, sparging, heating and cooling Airlift bioreactors Cell retention devices-filters, acoustic waves, inclined settlers Roller bottles Micro-carrier/fibrous disk reactors Hollow fiber bioreactors Wicking matrix bioreactor
Culture modes Batch culture Fed batch culture Perfusion culture Expansion (seed) train Continuous culture/continuous biomanufacturing SESSION 5 AGENDA - 2PM GMT, 8TH DECEMBER
Process measurement and control Examine the following parameters to monitor and control in your bioreactor Dissolved oxygen pH Temperature Probe insertion techniques Biomass Raman and other spectroscopic techniques Offline measurements Osmolarity, inhibitory metabolites Control loops CO2 sweeping Feed rates, bleed/recycle rates in perfusion culture
Case study 2-process deviation SESSION 6 AGENDA - 2PM GMT, 9TH DECEMBER
Single use bioreactors (SUB) Types of SUBS-rocking bags, stirred tanks, small rigid bioreactors SUB manufacturers Advantages and disadvantages of stainless steel vs. disposables
Scale-up Examine strategies to prepare for bioreactor scale-up Scale-up considerations, power, mixing, computational fluid dynamics (CFD) simulations Scale-out Scale-down models High throughput parallel reactors Lab-on-a –chip models
Case study 1 follow-up: consider how difficult it would be to scale-up each of the potential COVID-19 vaccine types SESSION 7 AGENDA - 2PM GMT, 10TH DECEMBER
Sterilization and cleaning Overview of sterilization principles to avoid contamination Heat sterilizationGas filtration Liquids filtration Examine the potential for raw materials viral contamination Sterilization in place (SIP) Cleaning in place (CIP) Sterile connections- tubing welding, flanged fittings For information contact our training consultants Elysia Ndubuisi [email protected] +44 (0)20 3377 3943
Jordanna Van Lint [email protected] +44 (20) 701 74734
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