Polyclonal Antibody to Plexin A4 (1630-1680)

AP32923PU-N OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Polyclonal Antibody to Plexin A4 (1630-1680) - Aff - Purified Alternate names: KIAA1550, PLXNA4, PLXNA4A, PLXNA4B, Plexin-A4 Catalog No.: AP32923PU-N Quantity: 0.1 mg Concentration: 0.5 mg/ml Background: Plexin A4 belongs to the plexin A-type group of proteins and serves as guidance molecules in the nervous system and show a novel influence on TLR signaling. Plexin A4 is the key determinant signaling coreceptor for Neuropilin 1. It forms a receptor complex with neuropilin 1 and can propagate semaphorin-3A elicited inhibitory signals into cells and neurons. This suggests Plexin A4 as a new intervention point for treating sepsis. Uniprot ID: Q9HCM2 NCBI: NP_001099013.1 GeneID: 91584 Host / Isotype: Rabbit / Ig Immunogen: A portion of amino acids 1630-1680 of Human Plexin A4 Remarks: The amino acid sequence used as immunogen is 100% homologous in human, new world monkey, panda, cow, dog, orangutan, opossum, lizard, wild turkey, zebra finch, society finch, chicken, parakeet, rhesus monkey, chimpanzee and horse, and 94% homologous in mouse, rat, zebrafish and western clawed frog. Format: State: Liquid purified Ig fraction Purification: Antigen Affinity Chromatography Buffer System: PBS Preservatives: 0.05% Sodium Azide Stabilizers: 0.05% BSA Applications: Western blot analysis: Use at 0.5-2 μg/ml. Recommended Positive Control: Brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This Peptide Affinity purified antibody recognizes Plexin A4. Species Reactivity: Tested: Chicken, Chimpanzee, Cow, Dog, Frog, Horse, Human. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Wen H, Lei Y, Eun SY, Ting JP. Plexin-A4-semaphorin 3A signaling is required for Toll-like receptor- and sepsis-induced cytokine storm. J Exp Med. 2010 Dec 20;207(13):2943-57. doi:

For research and in vitro use only. Not for diagnostic or therapeutic work. OG/20130829 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 AP32923PU-N: Polyclonal Antibody to Plexin A4 (1630-1680) - Aff - Purified 10.1084/jem.20101138. Epub 2010 Nov 22. PubMed PMID: 21098092. 2. Yaron A, Huang PH, Cheng HJ, Tessier-Lavigne M. Differential requirement for Plexin-A3 and -A4 in mediating responses of sensory and sympathetic neurons to distinct class 3 Semaphorins. Neuron. 2005 Feb 17;45(4):513-23. PubMed PMID: 15721238. Pictures: Western blot analysis using Plexin A4 antibody: Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with Plexin A4 antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.