PTK2 Antibody Cat. No.: 63-548
PTK2 Antibody
Western blot analysis of PTK2 using rabbit polyclonal PTK2 Formalin-fixed and paraffin-embedded human brain Antibody using 293 cell lysates (2 ug/lane) either tissue reacted with PTK2 Antibody , which was peroxidase- nontransfected (Lane 1) or transiently transfected with the conjugated to the secondary antibody, followed by DAB PTK2 gene (Lane 2). staining.
Confocal immunofluorescent analysis of PTK2 Antibody Flow cytometric analysis of K562 cells using PTK2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat (bottom histogram) compared to a negative control cell anti-rabbit lgG (green). Actin filaments have been labeled (top histogram)FITC-conjugated goat-anti-rabbit secondary with Alexa Fluor 555 phalloidin (red).DAPI was used to antibodies were used for the analysis. stain the cell nuclear (blue).
Specifications
September 25, 2021 1 https://www.prosci-inc.com/ptk2-antibody-63-548.html HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat, Chicken
This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 396-423 amino acids from the Central region of human PTK2.
TESTED APPLICATIONS: Flow, IF, IHC-P, WB
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 APPLICATIONS: For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
PREDICTED MOLECULAR 119 kDa WEIGHT:
Properties
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: PTK2
Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein ALTERNATE NAMES: phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, PTK2, FAK, FAK1
ACCESSION NO.: Q05397
PROTEIN GI NO.: 3183518
GENE ID: 5747
September 25, 2021 2 https://www.prosci-inc.com/ptk2-antibody-63-548.html USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix BACKGROUND: constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
REFERENCES: 1) Calalb,M.B., et.al., Mol. Cell. Biol. 15 (2), 954-963 (1995)
2) Polte,T.R. et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (23), 10678-10682 (1995)
3) Gervais,F.G., et.al., J. Biol. Chem. 273 (27), 17102-17108 (1998)
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
September 25, 2021 3 https://www.prosci-inc.com/ptk2-antibody-63-548.html