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Octet Software Version 10: Data Acquisition and Data Analysis High Octet Software Version 10.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT) Faster, more powerful data analysis Key features Octet Data Acquisition software An Experiment Wizard and built-in protocols guide users • Analyze multiple plates and experiments together through a step-by-step data acquisition process, facilitating • Customize PDF data reports using text, graphs, data tables both assay setup and efficient experimental design. Interactive and images sample plate maps allow rapid plate definition using simple click • More flexible and advanced reference subtraction options and drag mouse movements, and sample wells, standard wells, and preprocessing tools for kinetic data prior to analysis biosensors and regeneration steps are descriptively color- • Additional data correction options to choose from coded. During acquisition, data are displayed in real time with a detailed graphical interface that shows experimental progress. • New graphical display options to thicken sensor traces and Interactive zoom tools and advanced viewing options, such as customize axis scale and title options real-time reference subtraction and alignment of traces to a • Automated analysis for quantitation, kinetic and epitope bin- step or a certain time, are available while the experiment is run- ning data ning. NEW! Octet Data Acquisition software v10 also supports • Compatible with 32- and 64-bit versions of Windows® 10 the Octet RED96e system and the Biosensor Mount Cleaning Tool for Octet 384 and HTX systems. Upgrade information Octet Data Analysis HT software Octet® software provides an intuitive and easy to use inter- face for data acquisition and analysis on all Octet instruments, The Octet Data Analysis HT user interface is now more enabling label-free kinetic, affinity, epitope binning, activity, interactive and updates automatically when settings are concentration and screening applications. Note: Octet Data changed. The latest software version contains all analyses, Analysis HT software is not available in the Octet CFR software including quantitation, data processing and corrections, kinetics suite installer. To upgrade your software to v10.0, please com- and epitope binning. All setting parameters are saved locally in plete the software download form or contact your local sales a new .efrd file to ensure user-customized settings are stored. representative. DATA PREPROCESSING multiple plates that were run using similar experiment setups, the same reference subtraction and data corrections applied The software allows overlay of multiple experiments and plates to one plate can also be applied automatically to other plates for simplified viewing. Datasets can also be appended and in the dataset. analyzed simultaneously, reducing analysis time from hours to minutes. Hundreds to thousands of samples can be visualized, Advanced inter-step correction options remove bulk shifts or processed, analyzed and presented in the final report, so an artifacts to ensure data has been appropriately processed prior entire campaign or project to select leads from the global to fitting (Figure 3). dataset (Figure 1). Report point values can be added to any step in the sensor Octet Data Analysis HT software with an interactive interface trace, providing flexibility of comparing loading, baseline, as- offers more flexible tools for customizing data analysis that ca- sociation or dissociation levels between traces. This feature is ters to users at all experience levels. There are no restrictions especially useful in assessing loading levels, analyte asso- on reference sample(s) placement, and advanced options for ciation levels, as well as early and late dissociation levels in reference subtraction are available that remove any non-specif- screening applications such as ranking several clones based ic binding and/or baseline drift (Figure 2). For rapid analysis of of off-rates. Figure 1: 10 large molecule kinetics datasets overlaid and combined to significantly speed up analysis and viewing. Figure 3: Advanced data processing option to remove bulk shifts or assay artifacts. Figure 2: More flexible reference subtraction options. KINETICS ANALYSIS Simple 1-step and multi-step quantitation assays can be eas- ily analyzed with a few clicks. Extended data preprocessing Multiple interactions from several plates or experiments can options are available to perform one or multiple reference now be fitted and evaluated in parallel (Figure 4). Faster and subtractions depending on the assay setup. Once the data more optimized 1:1 and 2:1 algorithms enable better fitting with has been processed, binding rate is calculated based on initial lower residuals and rapid analysis for large datasets. slope or rate at equilibrium. The data can then be fitted using Once analysis is complete, customized reports can be created unweighted or weighted 4 parametric (4PL) 5PL or linear fits by combining various data elements such as graphs, text, data (Figure 7). tables, company logo, images and experimental details (Figure Automated sample alert tools are now available that analyze 5). Reports are then ready to be uploaded to an electronic note- data quality for the magnitude of precision between replicates, book or stored in the database. Report templates can be saved accuracy, dilution linearity and residuals, enabling interpretation and re-loaded to make similar reports for additional datasets. of results with confidence (Figure 8). Customized reports, similar to Figure 5, can also be generated. QUANTITATION ANALYSIS Once quantitation analysis is complete, customized reports can Octet Data Analysis HT software also enables multi-experiment be created by combining various data elements such as graphs, analysis of quantitation datasets that significantly speeds up text, data tables, company logo, images and experimental de- analysis of multiple plates (Figure 6). tails as described earlier. Reports are then ready to be upload- ed to an electronic notebook or stored in the database. Figure 4: Kinetic analysis on a combined dataset. Figure 5: Customized experimental report. Figure 6: Multi-experiment quantitation analysis. Figure 7: Quantitation binding rate calculation and curve fitting. Automated acquisition and analysis for all types of Octet data Octet Data Acquisition, Data Analysis and Data Analysis HT software provide support for an automation interface through a Serial Port (RS-232) or a Transmission Control Protocol/In- ternet Protocol (TCP/IP) socket for Octet QK384, RED384 and HTX instruments. Octet Data Analysis HT v10 software also enables automated analysis of all types of datasets, including quantitation, kinetic and epitope binning data which was not previously possible. The automation interface was designed to be as universal as possible, making no assumptions about the Figure 8: Sample alert tools. communication medium or the language of the client applica- tion connecting to Octet software. Octet Data Acquisition software version feature comparison Feature V8.x V9.x V10.x Experiment Wizard includes built-in protocol templates for kinetics, quantitation and epitope ü ü binning Capture Antibody step type in Advanced Quantitation for easier conversion of ELISA to Octet ü ü assays Visualization of all assay steps in advanced quantitation module ü ü ü Add multiple assay steps at one time in “Step Data List” in kinetics for quick assay setup ü ü ü In kinetics, all assay steps in multiple assays within an experiment can be displayed in a grid for ü ü ü better visualization Remote monitoring during experimental run ü ü ü Support for the Octet RED96e system ü Support for the Biosensor Mount Cleaning Tool for Octet 384 and HTX systems ü Support for microplate evaporation cover consumable on Octet RED96e system only ü Octet Data Analysis software version feature comparison Feature V8.x V9.x V10.x Quantitation Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Import standard curve of experiments run at the same temperature ü (Octet RED96e system only) Show temperature trace for the experiment ü Kinetics View all graphs in either logarithmic or linear scale ü ü ü Mask all proprietary information (sample ID, sensor info, etc.) from data ü ü Editable cells, customize and sort Results table ü ü Assign % KD1 and KD2 contribution to 2:1 model interaction ü ü Show temperature trace for the experiment ü Octet Data Analysis HT software version feature comparison Feature V8.x V9.x V10.x Epitope binning analysis Append or overlay data from multiple experiments, plates and biosensor trays ü ü Automatically create 2D traffic light matrix ü ü Highlight unidirectional binning pairs ü ü Flag Ab 1 traces with low response or no binding in the 2D matrix ü ü Subtract self-binding in the matrix ü Subtract the matrix against a select row or column ü Data preprocessing for kinetics and quantitation assays* Perform data processing for a combined mega dataset consisting of data from multiple ü experiments, plates and/or biosensor trays More flexible referencing options – by column, row, selected wells, pairs, pattern and between ü biosensor trays Additional referencing for quantitation assays – reference well and reference load well when no ü or irrelevant capture molecule is immobilized Advanced inter-step correction to remove bulk shifts or assay artifacts ü Report point feature for whole sensor traces – can be added in any step in the trace ü More graphical options – thicken line, change
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