The Journal of Cell Biology
JCB
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Dynamics andcalciumsensitivity oftheCa Dermott W. O’Callaghan, Alexei V. Tepikin, andRobert D.Burgoyne and Ca be essentialtohavedirectinformationontheirdynamics The translocation was dependentonbindingofCa photobleaching anduseofaphotoactivable GFPconstruct. Ca al., 1998;Pongset1993),regulationofCa with modulationofneurotransmitterrelease(McFerran et mainly inneuronsandneuroendocrinecellsarelinked (NCS) proteins(BurgoyneandWeiss,2001)arefound only invitroestimatesofCa whose invivoCa 1999; Deisserothetal.,1998;Milikan2002)and understanding ofwhichCa 2002). LittleisknownabouthowCa Calcium regulatesnumerouscellularprocessesandCa EF-hands 2and3.UsingphotolysisofNP-EGTA, themaximal Ca in responsetoincreasedcytosolic [Ca Physiological Laboratory, University ofLiverpool, Liverpool L693BX,England, UK switch protein hippocalcininliving cells differing Ca and Cronk,1999).ForotherEF-handCa global rise(Bootmanetal.,2001).ThesediverseCa can varyfrombrieflocalizedreleaseeventstoaprolonged fluorescent protein(EYFP)todeterminethedynamics and in HeLacellsexpressinghippocalcin–enhancedyellow Ca nals areinterpretedbyCa et al.,2002;Weiss2000;andBurgoyne,2002) activated underparticularCa Introduction Key words: hippocalcin;calciumsensors; EF-hand calcium;neurons; email: [email protected] Tel.: 44-151-794-5305.Fax:44-151-794-5337. Crown Street,UniversityofLiverpool, LiverpoolL693BX,England,UK. Robert D.Burgoyne,PhysiologicalLaboratory, to correspondence Address The onlineversionofthisarticleincludes supplementalmaterial. http://www.jcb.org/cgi/doi/10.1083/jcb.200306042 The Journal ofCellBiology
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