21.10.2014ACVR1C antibody (pAb)

Rabbit Anti-Human/Mouse/Rat Activin A Receptor Type IC (ACTR-IC, ACVRLK7, ALK7, ALK -7)

Instruction Manual

Catalog Number PK-AB718-4795 Synonyms ACVR1C Antibody: Activin A receptor type IC, -like 7, Activin receptor type 1C precursor, ACTR-IC, ACVRLK7, ALK7, ALK-7 Description Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine which include at least two type I and two type II receptors. ACVR1C, also known as ALK7, is a type I activin receptor and plays a role in cell differentiation, growth arrest and apoptosis. ACVR1C can mediate signaling by ligans such as Nodal, GDF-1/3, activin B and activin AB, all of which can also signal through the ubiquitous activin type I receptor ACVR1B (also known as ALK4). ACVR1C is a novel marker specifically expressed during the late phase of adipocyte differentiation. ACVR1C is dispensable for mouse embryogenesis, which suggests alternative functions for this receptor in postnatal development and tissue homeostasis. ACVR1C plays an important role in regulating the functional plasticity of pancreatic islets, negatively affecting beta-cell function by mediating the effects of activin B on Ca² ⁺ signaling. This antibody is predicted to have no cross-reactivity to ACVR1 or ACVR1B. Quantity 100 µg Source / Host Rabbit Immunogen ACVR1C antibody was raised against a 15 amino acid peptide near the amino terminus of the human ACVR1C. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity This antibody is predicted to have no cross-reactivity to ACVR1 or ACVR1B. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. ACVR1C antibody can be used for detection of ACVR1C by Western blot at 1 and 2 µg/mL. Images Available upon request. References Tsuchida K, Sawchenko PE, Nishikawa S, et al. Molecular cloning of a novel type I receptor serine/threonine kinase for the TGF beta superfamily from rat brain. Mol. Cell. Neurosci. 1996; 7:467–78. Reissmann E, Jornvall H, Blokzijl A, et al. The orphan receptor ALK7 and the activin receptor ALK4 mediate signaling by proteins during vertebrate development. Dev. 2001; 15:2010– 22. Tsuchida K, Nakatani M, Yamakawa N, et al. Activin isoforms signal through type I receptor serine/threonine kinase ALK7. Mol. Cell Endocrinol. 2004; 220:59-65. Kogame M, Matsuo S, Nakatani M, et al. ALK7 is a novel marker for adipocyte differentiation. J. Med. Invest. 2006; 53:238-45. Images Available upon request. Related Products Cat.No. PK-AB718-4795P - ACVR1C Peptide Cat. No. PK-AB718-1309 - Human Placenta Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014