NeoBiotechnologies, Inc. 2 Union Square Union City, CA 94587 Tel: 510-376-5603 Email: [email protected] , [email protected] Website: www.NeoBiotechnologies.com

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal [Clone 3D3]

Catalog No Format Size Price (USD)

5420-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00

5420-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00

5420-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 459.00

Human ID 5420 Immunogen A recombinant fragment containing the intracellular, transmembrane, and part of the extracellular domain of Human SwissProt O00592 human podocalyxin

Human Unigene 732423 Host / Ig Isotype Mouse / IgG1, kappa

Human Gene Symbol PODXL Mol. Weight of Antigen 165-170kDa

Human 7q32.3 Cellular Localization Cell surface and cytoplasmic Location Species Reactivity Human. Rabbit. Rat. Synonyms PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1 Positive Control HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney.

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Mouse Monoclonal Antibody (3D3).

Specificity & Comments Supplied As Podocalyxin is a member of the CD34 transmembrane sialomucin family. It 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. is over-expressed on the foot projections and plays essential roles Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available in kidney development and homeostasis, blood filtration and urine formation. WITHOUT BSA & azide at 1.0mg/ml. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more Storage and Stability aggressive tumor behavior. Podocalyxin antibody can identify in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 and other kidney pathology. to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Known Applications & Suggested Dilutions Limitations This antibody is available for research use only and is not approved for use in diagnosis.

Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Warranty Immunoprecipitation (1-2ug/500ug protein) There are no warranties, expressed or implied, which extend beyond this Western Blot (1-2ug/ml) description. Company is not liable for any personal injury or economic Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining loss resulting from this product. of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Key References

1. Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997). 2. Sassetti C et. al. J Exp Med187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009).

NBT00427