UPF3A (Human) Recombinant Protein (Q01)

Catalog # : H00065110-Q01 規格 : [ 10 ug ] [ 25 ug ] List All

Specification Application Image

Product Human UPF3A partial ORF ( NP_075387, 382 a.a. - 475 a.a.) Enzyme-linked Immunoabsorbent Assay Description: recombinant protein with GST-tag at N-terminal. Western Blot (Recombinant Sequence: SEDEQRWGKGPGQDRGKKGSQDSGAPGEAMERLGRAQRCDDSPAP protein) RKERLANKDRPALQLYDPGARFRARECGGNRRICKAEGSGTGPEKREE A Antibody Production

Host: Wheat Germ (in vitro) Protein Array

Theoretical MW 36.08 (kDa):

Preparation in vitro wheat germ expression system Method:

Purification: Glutathione Sepharose 4 Fast Flow

Quality Control 12.5% SDS-PAGE Stained with Coomassie Blue. Testing:

Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing. Instruction:

Note: Best use within three months from the date of receipt of this protein.

MSDS: Download

Datasheet: Download

Applications

Enzyme-linked Immunoabsorbent Assay

Western Blot (Recombinant protein)

Antibody Production

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Gene Information

Entrez GeneID: 65110

GeneBank NM_023011 Accession#:

Protein NP_075387 Accession#:

Gene Name: UPF3A

Gene Alias: HUPF3A,RENT3A,UPF3

Gene UPF3 regulator of nonsense transcripts homolog A (yeast) Description:

Omim ID: 605530

Gene Ontology: Hyperlink

Gene Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other OTTHUMP00000018789,UPF3 regulator of nonsense transcripts Designations: homolog A

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