Kromasil HILIC First Choice for Polar Compound Separation

Kromasil Peak performance in HPLC

Kromasil HILIC First choice for polar compound separation

MZ-Analysentechnik GmbH Barcelona-Allee 17 • D-55129 Mainz Tel +49 6131 880 96-0 Fax +49 6131 880 96-20 e-mail: [email protected] AUTHORIZED DISTRIBUTOR www.mz-at.de Optimal selectivity of polar compounds with an MS compatible phase

Kromasil HILIC-D is designed for the best selectivity of polar compounds. Traditionally, polar compounds like organic acids, nucleobases, and water soluble vitamins have been a challenge to separate on a standard reversed phase column like C18. Kromasil HILIC-D offers a phase that has true orthogonal selectivity when compared to a C18 column, normally giving an opposite elution order. The diol derivatized Kromasil HILIC-D provides excellent reproducibility when compared to HILIC columns based on standard bare silica. In addition to the orthogonal selectivity, Kromasil HILIC-D offers a robust column that is 100% MS compatible. The phase is low bleed and the solvents used when running your HPLC in HILIC mode are optimal for MS. Therefore the sensitivity can be 100 times better when using Kromasil HILIC-D over traditional reversed phase columns. When purifying polar compounds, Kromasil HILIC-D can be scaled up, offering the same great performance as in analytical scale. Take advantage of the gene- rous surface area which can provide high loading capacity.

To learn more, visit www.kromasil.com/hilic or contact us directly!

Availability

Product codes for Kromasil 60-5-HILIC-D 05 10 15 25

column column length diameter 50 mm 100 mm 150 mm 250 mm

D 2.1 mm S05HDD05 S05HDD10 S05HDD15 —

C 3.0 mm S05HDC05 S05HDC10 S05HDC15 —

A 4.6 mm S05HDA05 S05HDA10 S05HDA15 S05HDA25

P 10 mm — — — S05HDP25

Q 21.2 mm — — — S05HDQ25

R 30 mm — — — S05HDR25

Other sizes available upon request. Guard columns available for each column dimension.

Product characteristics

• Pore size¹: 60 Å • Particle size: 5 µm • Phase: HILIC-D • Surface area¹: 540 m²/g

1: bare silica data Inverted elution compared to reversed phase

naphthalene uracil Kromasil 100-5-C18

012345 Common chromatographic conditions Column size: 4.6 × 150 mm [min] Mobile Phase: acetonitrile/water (90/10) Flow rate: 1 ml/min naphthalene NH Temperature: ambient Kromasil 60-5-HILIC-D uracil Detection: UV @ 254 nm O N O H

012345 [min]

Tuning retention factor in HILIC mode

25 In contrast to reversed phase, retention time is increased with the amount of organic modiﬁer 20 in the mobile phase. cytosine 15 guanine

k' adenine 10 uracil thymine 5 Common chromatographic conditions Column: Kromasil 60-5-HILIC-D 4.6 × 150 mm Sample: nucleobases (see ﬁgure) 0 Mobile Phase: acetonitrile/ammonium acetate buffer, 100 mM, pH 6.3 60 70 80 90 100 Flow rate: 1 ml/min Temperature: ambient acetonitrile [%] Detection: UV @ 254 nm

Optimized MS sensitivity and compatibility

HO N OH Melamine & cyanuric acid analysis cyanuric acid N N OH In recent years, there were animal and human deaths due to the contamination of food and milk products with melamine. H2N N NH2 The addition of melamine to food or milk containing cyanuric melamine N N acid causes the production of melamine cyanurate which in turn creates kidney problems that can lead to death. NH2 Therefore the FDA has mandated that milk and prepared food be tested for melamine. Kromasil HILIC-D provides the proper selectivity and excellent MS compatibility to be able to analyze even the smallest amounts of melamine. 0369 [min] Chromatographic conditions Column: Kromasil 60-5-HILIC-D 2.1 × 100 mm Mobile Phase: acetonitrile/ammonium acetate buffer, 100 mM, pH 4.5 (95/5) Flow rate: 0.4 ml/min Temperature: 25°C Detection: +/-ESI TIC SIM Applications

Separation of vitamins

Chromatographic conditions Column: Kromasil 60-5-HILIC-D 4.6 × 150 mm Sample: vitamins: 1 = nicotinamide 1 2 = riboﬂavin (B₂) 4 6 3 = pyroxidine (B₆) 4 = p-aminobenzoic acid (PABA) 2 5 = nicotinic acid (B₃) 7 6 = ascorbic acid (C) 3 8 7 = thiamine (B₁) 8 = cobalamin (B₁₂) 5 9 = folic acid (B₉) 9 Mobile Phase: acetonitrile/ammonium acetate buffer, 100 mM, pH 6.3 gradient: 0 min: 75%, 10 min: 55% acetonitrile Flow rate: 1.0 ml/min Temperature: ambient 0 2 4 6 8 10 Detection: UV @ 254 nm [min]

Separation of nucleobases

1 3 4 Chromatographic conditions Column: Kromasil 60-5-HILIC-D 4.6 × 150 mm 5 Sample: nucleobases: 1 = thymine 2 = uracil 3 = adenine 4 = guanine 5 = cytosine Mobile Phase: acetonitrile/ammonium acetate buffer, 100 mM, pH 6.3 (85/15) Flow rate: 2 ml/min 012345 Temperature: ambient [min] Detection: UV @ 254 nm

Separation of food additives

1 2

4 Chromatographic conditions Column: Kromasil 60-5-HILIC-D 4.6 × 150 mm 3 Sample: food additives: 1 = benzoic acid (E210) 2 = maleic acid (E296) 3 = ascorbic acid (E300) 4 = fumaric acid (E297) Mobile Phase: acetonitrile/ammonium formate buffer, 20 mM, pH 3.4 (75/25) Flow rate: 1 ml/min Temperature: ambient 012345 Detection: UV @ 254 nm [min]

The moment you adopt our Kromasil High Performance Concept, you join thousands of chromatographers who share a common goal: to achieve better separations when analyzing or isolating pharmaceuticals or other substances.

Not only will you benefit from our patented silica technology, but you gain a strong partner with a reliable track record in the field of silica products. For the past 70 years, we have pioneered new types of silica. Our long experience in the field of silica chemistry is the secret behind the development of Kromasil, and the success of our Separation Products group. Kromasil is available in bulk and in high-pressure slurry- packed columns. The development, production and marketing of Kromasil are ISO 9001 certified.

Kromasil is a brand of AkzoNobel, the largest global paint and coatings company and MZ-Analysentechnik GmbH a major producer of specialty chemicals with headquarters in Amsterdam, the Netherlands. With 55 000 people in more than 80 countries around the world, we are Barcelona-Allee 17 • D-55129 Mainz committed to sustainability, excellence and delivering Tomorrow’s Answers Today™. Tel +49 6131 880 96-0 Fax +49 6131 880 96-20 ® e-mail: [email protected] Kromasil AUTHORIZED DISTRIBUTOR www.mz-at.de