N-WASP/WASL Rabbit Pab

Leader in Biomolecular Solutions for Life Science

N-WASP/WASL Rabbit pAb

Catalog No.: A2576 1 Publications

Basic Information Background

Catalog No. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. A2576 Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is Observed MW highly expressed in neural tissues, and interacts with several proteins involved in 65kDa cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in Calculated MW the formation of long actin microspikes, and in neurite extension. 54kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 8976 O00401 IHC 1:50 - 1:100 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human N-WASP/WASL/WASL (NP_003932.3).

Synonyms WASL;N-WASP;NWASP;WASPB

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using N-WASP/WASL/WASL antibody (A2576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using N- embedded human colon carcinoma using embedded mouse heart using N- WASP/WASL/WASL antibody (A2576) at N-WASP/WASL/WASL antibody (A2576) at WASP/WASL/WASL antibody (A2576) at dilution of 1:100 (40x lens). dilution of 1:100 (40x lens). dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells Immunofluorescence analysis of HeLa cells Immunofluorescence analysis of L-929 using N-WASP/WASL/WASL Polyclonal using N-WASP/WASL/WASL Polyclonal cells using N-WASP/WASL/WASL Antibody (A2576) at dilution of 1:100 (40x Antibody (A2576) at dilution of 1:100 (40x Polyclonal Antibody (A2576) at dilution of lens). Blue: DAPI for nuclear staining. lens). Blue: DAPI for nuclear staining. 1:100 (40x lens). Blue: DAPI for nuclear staining.