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Phototoxic Risk Assessments on Derivatives: Photobiochemical

Assessments and Dermal Cassette-dosing Pharmacokinetic Study

Yoshiki Seto, Hiroto Ohtake, Masashi Kato, and Satomi Onoue*

Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Downloaded from Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan

jpet.aspetjournals.org

at ASPET Journals on September 24, 2021

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RUNNING TITLE

Phototoxic risk assessments on benzophenone derivatives

CORRESPONDING AUTHOR

Satomi Onoue, Ph.D.

Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Downloaded from Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan

Tel.: +81-54-264-5630, Fax: +81-54-264-5636, E-mail: [email protected]

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DOCUMENT STATISTICS

Number of text pages: 35 pages

Number of tables: 2 tables at ASPET Journals on September 24, 2021

Number of figures: 4 figures

Number of references: 29 references

Number of words: 203 words (in Abstract)

740 words (in Introduction)

1339 words (in Discussion)

ABBREVIATIONS

3T3 NRU PT, 3T3 neutral red uptake phototoxicity test; AUC0-24, area under the concentration versus time curve from 0 to 24 h; BZ, benzophenone; BZP, benzophenone derivative; CIE, Commission Internationale de l’Eclairage; Clog P, calculated log P;

Cmax, maximum concentration; DMSO, dimethyl sulfoxide; DO, ; EBSS,

Earle’s balanced salt solution; EM, erythromycin; EMA, European Medicines Agency;

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FDA, Food and Drug Administration; ICH, International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use; KT, ketoprofen; MEC, molar extinction coefficient; MRT, mean residence time; MX, ; NaPB, sodium phosphate buffer; NBT, nitroblue tetrazolium; OECD, the

Organisation for Economic Co-operation and Development; OX, ; PIF, photo irritancy factor; PK, pharmacokinetic; QN, quinine HCl; ROS, reactive oxygen Downloaded from species; SB, ; SIR, selected ion recording; UPLC/ESI-MS, ultra-performance liquid chromatography equipped with electrospray ionization mass

spectrometry; UV, ; VIS, visible light jpet.aspetjournals.org

at ASPET Journals on September 24, 2021

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ABSTRACT

This study aimed to qualify photosafety screening on the basis of photochemical and pharmacokinetic (PK) data on dermally applied chemicals. Six benzophenone derivatives (BZPs) were selected as model compounds, and in vitro photochemical/phototoxic characterization and dermal cassette-dosing PK study were carried out. For comparison, an in vivo phototoxicity test was also conducted. All of Downloaded from the BZPs exhibited strong UVA/B absorption with molar extinction coefficients of over

2000 M-1·cm-1, and benzophenone and ketoprofen exhibited significant ROS generation

2

upon exposure to simulated sunlight (ca. 2.0 mW/cm ); however, ROS generation from jpet.aspetjournals.org sulisobenzone and dioxybenzone was negligible. To verify in vitro phototoxicity, a

3T3 neutral red uptake phototoxicity test was carried out, and benzophenone and

ketoprofen were categorized to be phototoxic chemicals. The dermal PK parameters at ASPET Journals on September 24, 2021 of ketoprofen were indicative of the highest dermal distribution of all BZPs tested. On the basis of its in vitro photochemical/phototoxic and PK data, ketoprofen was deduced to be highly phototoxic. The rank of predicted phototoxic risk of BZPs on the basis of the proposed screening strategy was almost in agreement with the results from the in vivo phototoxicity test. The combined use of photochemical and cassette-dosing PK data would provide reliable predictions of phototoxic risk for candidates with high productivity.

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1. Introduction

Drug-induced phototoxicity is caused after exposure of light-exposed tissues to topically and/or systemically administered chemicals, including pharmaceuticals, cosmetics, and food ingredients, followed by exposure to sunlight, consisting of ultraviolet (UV) B (290–320 nm), UVA (320–400 nm), and visible light (VIS; 400–700 nm) (Moore, 1998; 2002; Onoue et al., 2009). Recently, interest in phototoxic events Downloaded from due to photoreactive substances has markedly increased owing to high-intensity UV rays from the sun reaching the Earth’s surface upon the destruction of the ozone layer

(Onoue et al., 2009). Perceptible adverse events would leave patients and consumers jpet.aspetjournals.org with negative impressions of products, and the pharmaceutical and cosmetic industries have struggled to predict and/or avoid phototoxic events. In the early 2000s,

regulatory agencies, including the Food and Drug Administration (FDA), the European at ASPET Journals on September 24, 2021

Medicines Agency (EMA), and the Organisation for Economic Co-operation and

Development (OECD), established guidance on photosafety testing of medicinal products (EMEA/CPMP, 2002; 2008; FDA/CDER, 2002; OECD, 2004). In 2014, the

International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) established the ICH S10 guideline (ICH,

2014), and this guidance describes photosafety assessment strategies on the basis of photochemical/photobiological properties and in vivo pharmacokinetic (PK) behaviors in UV-exposed tissues, such as skin and eyes.

Over the past few years, a number of efforts have been made to develop effective phototoxic assessments, and analytical and biochemical methodologies for evaluating phototoxic risk have been proposed, including in silico prediction models, photochemical screening tools, and in vitro phototoxicity assessments (Seto et al., 2012).

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In particular, UV/VIS measurement (Henry et al., 2009), 3T3 neutral red uptake phototoxicity test (3T3 NRU PT) (Spielmann et al., 1994), and/or reactive oxygen species (ROS) assay (Onoue and Tsuda, 2006; Onoue et al., 2008) are currently described as recommended methodologies for evaluating the photosafety of test substances, and these photosafety assessment tools are also useful in the early stages of product development. Although effective in vitro photosafety assessments have been Downloaded from developed on the basis of photochemical/photobiological mechanisms, the data obtained from these methodologies cannot fully reveal the occurrence of phototoxic events in a

clinical context due to a lack of data on distribution and retention in UV-exposed tissues. jpet.aspetjournals.org

Previously, combined use of in vitro photochemical/phototoxic data and in vivo PK behaviors was proposed for evaluating the in vivo phototoxic risk of chemicals (Seto et

al., 2009), and a cassette-dosing PK study was employed with the aim of reducing the at ASPET Journals on September 24, 2021 use of research resources and enhancing throughput (Onoue et al., 2014a; Seto et al.,

2011). In these previous studies, the predicted in vivo phototoxic risk of systemically exposed chemicals on the basis of the in vitro photochemical/photobiological and in vivo PK data exhibited a strong association with clinical phototoxicity findings (Onoue et al., 2014a; Seto et al., 2011). On the other hand, the proposed screening strategy has not been applied to phototoxic risk assessment on dermally applied chemicals, the phototoxicity of which may lead to a no-go decision in product development.

The present study aimed to clarify the applicability of the photosafety screening using photochemical and PK data for dermally administered chemicals. Six benzophenone derivatives (BZPs), namely, benzophenone (BZ), dioxybenzone (DO), ketoprofen (KT), mexenone (MX), oxybenzone (OX), and sulisobenzone (SB), were selected as model chemicals (Fig. 1). The phototoxic skin reactions by KT, MX, and

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OX have been reported on the basis of the results from human photopatch tests (Chuah et al., 2013; Hernando et al., 2013), although these are directly applied to the skin. OX,

SB, DO, and MX were developed as (Baughman et al., 2009; Darvay et al.,

2001), and KT is one of the most well-known non-steroidal anti-inflammatory drugs given via the dermal route. In this investigation, UV spectral analysis and ROS assay were carried out in order to evaluate the photochemical properties of BZPs, and 3T3 Downloaded from NRU PT was also undertaken to verify the in vitro phototoxicity of BZPs.

Cassette-dosing PK study in rats after the dermal co-administration of BZPs was

conducted to determine the PK behaviors of BZPs in the skin, and the skin jpet.aspetjournals.org concentration of BZPs was assessed by ultra-performance liquid chromatography equipped with electrospray ionization mass spectrometry (UPLC/ESI-MS). On the

basis of the obtained in vitro photochemical/photobiochemical and in vivo dermal PK at ASPET Journals on September 24, 2021 data, the in vivo phototoxic risk of the tested BZPs was rated. To compare the predicted in vivo phototoxic risk with the in vivo phototoxic observations, in vivo phototoxicity test were also performed on each BZP.

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2. Materials and methods

2.1. Chemicals

BZ was purchased from Junsei Chemical (Tokyo, Japan). DO, KT, MX, OX, erythromycin (EM), dimethyl sulfoxide (DMSO), imidazole, nitroblue tetrazolium

(NBT), p-nitrosodimethylaniline, propylene glycol, disodium hydrogen phosphate

12-water, and sodium dihydrogen phosphate dihydrate were obtained from Wako Pure Downloaded from Chemical Industries (Osaka, Japan). Quinine HCl (QN) and SB were purchased from Sigma-Aldrich Japan (Tokyo, Japan). Acetonitrile was obtained from

Honeywell International (Morristown, NJ, USA). jpet.aspetjournals.org

2.2. UV/VIS spectral analysis

Each chemical was dissolved in 20 mM sodium phosphate buffer (NaPB, at ASPET Journals on September 24, 2021 pH7.4) at a final concentration of 20 μM. UV/VIS absorption spectra were recorded with a Hitachi U-2010 spectrophotometer (Hitachi High-Technologies Corporation,

Tokyo, Japan) interfaced to a PC for data processing (software: Spectra Manager). A spectrofluorimeter quartz cell with 10 mm pathlength was employed.

2.3. ROS assay

2.3.1. Irradiation conditions

Chemicals were stored in an Atlas Suntest CPS+ solar simulator (Atlas

Material Technology LLC, Chicago, IL, USA) equipped with a xenon arc lamp (1500

W) and cooling unit SR-P20FLE (Hitachi, Tokyo, Japan). A UV special filter

(#56052371, Atlas) was installed to adapt the spectrum of the artificial light source to natural daylight, and the Atlas Suntest CPS series had a high irradiance capability that

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met CIE85/1989 daylight simulation requirements. The irradiation test was carried out at 25°C with irradiance of ca. 2.0 mW/cm2, as determined using the calibrated

UVA detector Dr. Hönle #0037 (Dr. Hönle, Munich, Germany).

2.3.2. Assay procedure

For the detection of both singlet oxygen and superoxide generation from Downloaded from irradiated compounds, the ROS assay was carried out according to the validated protocol with minor modification (Onoue et al., 2013; Onoue et al., 2014b). Briefly,

each tested compound was dissolved in DMSO at 10 mM for stock solution. For the jpet.aspetjournals.org determination of singlet oxygen generation, compounds (200 µM), p-nitrosodimethylaniline (50 µM), and imidazole (50 µM) were dissolved in 20 mM

NaPB (pH7.4). To monitor the generation of superoxide, compounds (200 µM) and at ASPET Journals on September 24, 2021

NBT (50 µM) were dissolved in 20 mM NaPB (pH7.4). Each sample was mixed in a tube, and then 200 μL of sample was transferred into a well of a plastic 96-well microplate (Asahi Glass, Tokyo, Japan; code number: 3881-096; clear, untreated, flat-bottomed). The samples were checked for precipitation before irradiation, and the plate fixed in the reaction container with a quartz cover was irradiated with simulated sunlight for 60 min. Before and after irradiation, absorbance levels at 440 nm and 560 nm were measured using SAFIRE (TECAN, Männedorf, Switzerland) for determination of singlet oxygen and superoxide generation, respectively.

2.4. 3T3 NRU PT

The in vitro 3T3 NRU PT was carried out as described in the OECD test guidelines 432 with minor modification (OECD, 2004). Briefly, Balb/c 3T3 cells

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were maintained in culture for 24 h for the formation of monolayers. Two 96-well plates per test chemical were then pre-incubated with six different concentrations of the chemical dissolved in Earle’s Balanced Salt Solution (EBSS) for 1 h in duplicate.

One plate was then exposed to a dose of 5 J/cm2 UVA (+Irr experiment), whereas the other plate was kept in the dark by covering it with aluminum foil (-Irr experiment).

UVA irradiation was performed using a sol 500 Sun simulator (Dr. Hönle) equipped Downloaded from with a 500 W metal halide lamp and a H-1 filter to remove potentially cytotoxic UVB wavelengths. The treatment medium was then replaced with culture medium and,

after 24 h, cell viability was determined by neutral red uptake for 3 h. After that, jpet.aspetjournals.org cells were lysed in eluate (ethanol:water:acetic acid=50:49:1), and the neutral red uptake was measured at the absorbance of 540 nm using the BenchmarkTM Plus

microplate spectrophotometer (BioRad, Hercules, CA, USA). Cell viability obtained at ASPET Journals on September 24, 2021 with each of the six concentrations of the test chemical was compared with that of untreated controls, and the percent inhibition was calculated. For evaluating in vitro phototoxicity, the concentration responses obtained in the presence and in the absence of UVA irradiation were compared, usually at the IC50 level, that is, the concentration inhibiting cell viability by 50% compared with that of untreated controls. The photo irritancy factor (PIF) was determined using the following equation:

IrrIC )-( PIF = 50 + 50 IrrIC )(

2.5. In vivo preparation

Male Sprague-Dawley rats at 10–12 weeks of age (ca. 309–440 g body weight) were purchased from SLC Inc. (Hamamatsu, Japan), housed in the laboratory with free access to food and water, and maintained on a 12-h dark/light cycle in a

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room with controlled temperature (24±1°C) and humidity (55±5%). On the day before experiments, rats were anesthetized with pentobarbital Na (50 mg/kg), and then the hair on the abdomen was shaved (5 cm×5 cm). All of the procedures used in the present study were conducted according to the guidelines approved by the Institutional

Animal Care and Ethical Committee of the University of Shizuoka.

Downloaded from

2.6. Skin deposition of BZPs after dermal co-administration

A cocktail solution containing all 6 BZPs (final concentration of each: 1 mg/mL) was prepared with the use of propylene glycol and applied to the rat skin over jpet.aspetjournals.org the abdomen (0.1 mg/rat each, n=5) using an adhesive plaster for a patch test

(Libatape Pharmaceutical, Kumamoto, Japan) under anesthesia with pentobarbital Na

(50 mg/kg). At the indicated times (2, 4, 6, 8, 12, and 24 h) after the dermal at ASPET Journals on September 24, 2021 co-administration of BZPs, rats were humanely killed by taking blood from the descending aorta under anesthesia with pentobarbital Na (50 mg/kg), and the tissues were then perfused with cold saline from the aorta. The skin was dissected, minced with scissors, and homogenized using Physcotron (Microtec, Chiba, Japan) in 4 mL of acetonitrile. After sonication for 10 min and shaking for 10 min, the samples were centrifuged (3000 rpm, 10 min). Extraction was repeated twice with acetonitrile, and the supernatants were pooled. The collected eluents were pooled with acetonitrile extracts, and the samples were evaporated to dryness under a gentle stream of nitrogen at 45°C. The residues were dissolved in 50% acetonitrile including fenofibric acid

(500 ng/mL) as an internal standard for UPLC analysis.

2.7. UPLC analysis

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The concentration of BZPs in rat skin was determined by UPLC-ESI/MS analysis. The UPLC-ESI/MS system consisted of a Waters Acquity UPLC system

(Waters, Milford, MA, USA), which included a binary solvent manager, a sample manager, a column compartment, and a micromass SQ detector connected with Waters

Masslynx v 4.1. A Waters Acuity UPLC BEH C18 (particle size: 1.7 μm and column size: Φ2.1×50 mm; Waters) was used, and the column temperature was maintained at Downloaded from 40°C. The standards and samples were separated using a gradient mobile phase consisting of Milli-Q containing 0.1% formic acid (A) and acetonitrile (B). The

gradient conditions of the mobile phase were 0–1 min, 50% B; 1–3 min, 50–75% B jpet.aspetjournals.org

(linear gradient curve); 3–4 min, 75–95% B (linear gradient curve); 4–5.5 min, 95% B; and 5.5–6 min, 50% B, and the flow rate was set at 0.25 mL/min. Analysis was

carried out using selected ion recording (SIR) for specific m/z 183.2 for BZ [M+H]+, at ASPET Journals on September 24, 2021

255.2 for KT [M+H]+, 229.2 for OX [M+H]+, 307.1 for SB [M-H]-, 245.2 for DO

[M+H]+, 243.3 for MX [M+H]+, and 319.2 for fenofibric acid [M+H]+ as an internal standard. Peaks for BZ, KT, OX, SB, DO, MX, and fenofibric acid were detected at retention times of 1.96, 1.14, 2.54, 0.57, 2.54, 3.18, and 2.00, respectively. The newly developed UPLC-ESI/MS method for the determination of BZPs was validated in terms of linearity, accuracy, and precision according to the ICH guidelines “Q2B Validation of

Analytical Procedures: Methodology.” On the basis of the skin concentration obtained,

PK analysis was performed, and the area under concentration versus time curve from 0 to 24 h (AUC0–24) and mean residence time (MRT) were estimated using a trapezoid formula.

2.8. In vivo phototoxicity testing

Each BZP or control (QN and EM) was dissolved in DMSO at 100 mg/mL,

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and was applied to 2 application sites on rat skin at the abdomen (10 mg/site, n=4–5) using filter paper (2 cm×2 cm) under anesthesia with pentobarbital Na (50 mg/kg).

The exposure time before irradiation was set at 3 h after dermal administration in order to avoid a long time of restraint and to reduce the risk of death by supplemental anesthesia. At 3 h after dermal administration, the filter papers containing chemicals on the application sites were removed and wiped using cotton soaked with distilled Downloaded from water. Then, rats were irradiated individually using black light (FL15BL-B, National,

Tokyo, Japan) as a UVA light source with an irradiance of ca. 2.7 mW/cm2 for ca. 3 h

2 until the UV irradiance level reached 30 J/cm . Because UVB light is highly jpet.aspetjournals.org cytotoxic, a UVA light source was employed for the in vivo phototoxicity testing.

During the UVA irradiation, rats were restrained on a sunbed under anesthesia with

pentobarbital Na (50 mg/kg) to ensure uniform irradiation of their abdomen, and at ASPET Journals on September 24, 2021 non-irradiated sites were wrapped in aluminum foil for protection from UV light.

UV intensity was monitored using the calibrated UVA detector Dr. Hönle no. 0037 (Dr.

Hönle). A colorimeter equipped with a data processor (NF333, Nippon Denshoku,

Tokyo, Japan) was used as a measure of skin color. This instrument records

3-dimensional color reflectance, so-called L*a*b* system, as recommended by the

Commission Internationale de l’Eclairage (CIE). The luminance (L*) gives the relative brightness ranging from total black (L* = 0) to total white (L* = 100). The hue

(a*) axis represents the balance between red (positive values up to 100) and green

(negative values up to −100), and the chroma (b*) axis represents the balance between yellow (positive values up to 100) and blue (negative values up to −100). The differences in skin color (ΔE) between before and after irradiation were described as follows (Pierard and Pierard-Franchimont, 1993; Westerhof et al., 1986):

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()()()2 2 Δ+Δ+Δ=Δ baLE *** 2

2.9. Data analysis

For statistical comparisons, one-way ANOVA with pairwise comparison by

Fisher’s least significant difference procedure was used. A p value of less than 0.05 was considered significant for all analyses. Theoretical calculations of the lipophilicity Downloaded from as calculated log P (Clog P) were performed using ChemBioDraw Ultra 13.0

(PerkinElmer, Waltham, MA, USA) with chemical structure inputs. jpet.aspetjournals.org at ASPET Journals on September 24, 2021

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3. Results

3.1. Photochemical characterization of BZPs

As a first step of phototoxic events, phototoxic compounds are transferred from their ground state to an excited state by absorption of photon energy from sunlight, and

UV/VIS spectral analysis in the range of 290–700 nm is recommended by guidelines as a rapid and simple method for predicting the photoreactive/phototoxic potential of Downloaded from chemicals (Seto et al., 2012). Thus, the UV/VIS spectral patterns of 6 BZPs (20 μM) were recorded in 20 mM NaPB (pH7.4). All BZPs exhibited intense absorption in the

UVA/B range (Fig. 2A); in contrast, absorption spectral patterns of BZPs could not be jpet.aspetjournals.org observed in the VIS region (data not shown). On the basis of the spectral patterns obtained, molar extinction coefficient (MEC) values of BZPs at 290 nm were calculated

as follows: 2,317 (BZ), 10,833 (DO), 4,350 (KT), 2,850 (MX), 6,817 (OX), and 11,700 at ASPET Journals on September 24, 2021

(SB) M-1・cm-1. According to the previous report (Henry et al., 2009) and ICH S10 photosafety guidance (ICH, 2014), compounds with MEC values greater than 1,000

M-1・cm-1 at any wavelength (290–700 nm) are considered to be of greater photosafety risk. Herein, all tested BZPs were thus suggested to have photoexcitability and photoreactive potential, possibly leading to phototoxic reactions.

In the indirect process of phototoxic events, ROS including singlet oxygen and superoxide are well known as principal intermediate species, and oxidative damage against biomolecules by chemicals after sunlight exposure mainly arises via ROS, resulting in various phototoxic events (Epstein, 1983). Thus, monitoring ROS generation from photoirradiated chemicals would be useful for evaluating the phototoxic risk of chemicals. To clarify the photoreactivity of BZPs, the ROS assay

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was undertaken with the use of 6 BZPs at 200 μM, and QN and EM (200 μM) were employed as positive and negative controls for ROS generation, respectively (Fig. 2B).

KT and QN exhibited significant generation of both singlet oxygen and superoxide under simulated sunlight exposure, and BZ and OX yielded singlet oxygen via a type II photochemical reaction. In contrast, ROS generation from DO, SB, and EM was negligible, and the ROS data on MX could not be obtained owing to the appearance of Downloaded from precipitation of MX. As for poorly water-soluble chemicals, a lower concentration of chemicals can be applied to the ROS assay for evaluating photoreactivity; therefore, for

further investigation, ROS assay on MX at 100 μM was also carried out. The jpet.aspetjournals.org generation of singlet oxygen from irradiated MX could be detected without the

3 precipitation of MX (ΔA440 nm・10 : 67), whereas the generation of superoxide anion from irradiated MX (100 μM) was negligible. The chemicals can be determined to be at ASPET Journals on September 24, 2021 photoreactive if the obtained ROS data surpass the previously defined criteria in the

3 3 ROS assay (singlet oxygen [ΔA440 nm・10 ]: 25; and superoxide [ΔA560 nm・10 ]: 20)

(Onoue et al., 2008; Onoue et al., 2014b). On the basis of these criteria, BZ, KT, MX, and OX were found to be photoreactive, and these BZPs would cause phototoxic reactions in the skin. DO and SB were judged to be less photoreactive by ROS assay, whereas, interestingly, their MEC values were higher than those of the other BZPs generating ROS. These findings might suggest that UV/VIS absorption properties would not always reflect the rank-order photoreactivity/phototoxicity of chemicals.

3.2. In vitro phototoxicity of BZPs

For evaluating in vitro phototoxicity, 3T3 NRU PT, which is widely used as an

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alternative in vitro methodology to various in vivo photosafety assessments, was conducted. The 3T3 NRU PT was developed and validated under the auspices of

ECVAM from 1992 to 1997 (Liebsch and Spielmann, 2002), and the OECD recommend this in vitro methodology for evaluating the photosafety of chemicals (OECD, 2004).

The 3T3 NRU PT assesses the concentration-dependent cytotoxicity on the Balb/c 3T3 mouse fibroblast cell line of UVA-irradiated chemicals using the uptake of neutral red Downloaded from by living cells. The cell viability curves of BZPs with or without UVA irradiation were obtained, and the PIF values were calculated (Table 1). Compared with the

non-irradiated groups, UVA-irradiated BZ and KT exhibited significant cytotoxicity at jpet.aspetjournals.org lower doses, and the viability curves of UVA-irradiated groups were shifted to the left.

The PIF values of BZ and KT were estimated to be 49.5 or more and 68.9, respectively.

In contrast, the PIF values of DO, OX, and SB were calculated to be ca. 1.0 since these at ASPET Journals on September 24, 2021 chemicals produced similar cell viability curves between UVA-irradiated and non-irradiated groups. The cytotoxicity of MX could not be fully monitored owing to its poor solubility to the assay mixture, and the PIF values of MX could not be calculated; therefore, 3T3 NRU PT would not be suitable for evaluating the phototoxicity of MX. According to the OECD test guidelines 432, classification criteria of 3T3 NRU PT on the basis of PIF values are defined as follows: (i) phototoxicity (PIF≥5); (ii) probable phototoxicity (2≤PIF ＜ 5); and (iii) non-phototoxicity (PIF＜2) (OECD, 2004). Thus, BZ and KT were identified as phototoxic molecules, and DO, OX, and SB were determined as non-phototoxic ones.

3.3. Pharmacokinetic behaviors of BZPs

The deposition of chemicals to sunlight-exposed tissues including skin and

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eyes was shown to be closely associated with the incidence of drug-induced phototoxic reactions (Seto et al., 2012), and the ICH S10 photosafety guidance describes in vivo PK behaviors as important factors of drug-induced phototoxicity (ICH, 2014); therefore, monitoring the skin concentration of dermally administered chemicals should be a key consideration for phototoxic risk prediction. In the present investigation, dermal cassette-dosing PK study was employed for improving the throughput of experiments Downloaded from and reducing the use of various research resources. In particular, considering implementation of the 3Rs principles (refinement, reduction, and replacement),

cassette-dosing approaches can make a strong contribution to reducing the number of jpet.aspetjournals.org animals humanely killed (Allen et al., 1998). The concentration versus time curves in the skin after dermal co-administration of the 6 BZPs (0.1 mg/rat each) were obtained

by UPLC-ESI/MS analysis (Fig. 3), and the PK parameters of the BZPs were also at ASPET Journals on September 24, 2021 calculated on the basis of the concentration-time profiles obtained (Table 1). With regard to BZ, the skin concentration reached the maximum concentration (Cmax) at 4 h after dermal co-administration (ca. 6.1 μg/g tissue) and then decreased steadily. As for

DO, MX, and OX, these 3 BZPs gradually increased their skin concentration, and each of their skin concentrations almost reached Cmax by ca. 4 h after dermal co-administration. The exposure levels of the skin to these 3 BZPs were maintained around each of their Cmax until ca. 8 h after dermal co-administration, and then they were eliminated from the skin. From these findings, the phototoxic risk of the 3 BZPs would persist for 4 to 8 h. Unlike the PK behaviors of the other 4 BZPs, the skin depositions of KT and SB reached their Cmax at 24 h after dermal co-administration, and their elimination phase could not be observed under the present experimental conditions.

The skin concentration of KT, in particular, remained around its Cmax value (8.7 μg/g

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tissue) from 8 to 24 h after dermal co-administration, and the AUC0–24 and MRT of KT were estimated to be 160.0 h・μg/g tissue and over 14.2 h, respectively; therefore, KT would be associated with higher and longer exposure of the skin after dermal administration than the other BZPs. Previously, long skin retention of KT after dermal administration was observed in guinea pigs (Nakazawa et al., 2006), and the present outcomes are in agreement with this previous report. SB also exhibited the highest Downloaded from MRT value (＞14.9 h) among the tested BZPs, affecting the duration of exposure risk of the skin to SB.

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3.4. In vivo phototoxicity on BZPs

To confirm the assessment capability of the photosafety screening, the in vivo phototoxicity of BZPs was also evaluated. In this study, in vivo phototoxicity testing at ASPET Journals on September 24, 2021 in rat skin after dermal administration of each BZP, QN (positive control), and EM

(negative control) at a dose of 10 mg/site was also conducted for screening purposes.

On the basis of the dermal cassette-dosing PK study, most of tested BZPs reached Cmax

(BZ) or near Cmax (DO, MX, and OX) at around 4 h; therefore, exposure of the skin to tested BZPs for 3 h were undertaken, and then, UVA irradiation was conducted for ca. 3 h in the in vivo phototoxicity test. On the other hand, in the present experiment sequence, the skin deposition of KT and SB might not be sufficient for induction of phototoxicity on the basis of the obtained PK data. To avoid insufficient skin exposure to tested BZPs, the 100-fold higher doses for dermal application were employed in the in vivo phototoxicity test compared with those in the cassette-dosing PK study.

UVA-induced cutaneous inflammation was monitored by changes in skin color, and skin color was measured before and after UVA irradiation using a colorimeter with the

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L*a*b* system (Fig. 4). In the L*a*b* system, L* represents brightness of shade, and a* and b* represent the amount of red-green and yellow-blue color, respectively. In colorimetrical evaluation of the skin surface, QN was associated with a significant difference in ΔE values between irradiated and non-irradiated sites. The difference of

ΔE values was calculated to be ca. 7.5 and was almost the same value as for orally administered QN in the previous in vivo phototoxicity test (Onoue et al., 2014a). In Downloaded from contrast, the difference in ΔE values of EM between irradiated and non-irradiated sites was not significant, and EM might not induce phototoxic events in the present

conditions; therefore, the in vivo phototoxicity test would have been adequately carried jpet.aspetjournals.org out, and the obtained outcomes may be reliable to some extent. The ΔE values of BZ and KT in the irradiated groups were significantly different from those in the corresponding non-irradiated groups, and irradiated MX tended to exhibit a high ΔE at ASPET Journals on September 24, 2021 value compared with non-irradiated MX (p=0.0607). Thus, BZ and KT would have potent in vivo phototoxicity, and MX was determined to be a moderately phototoxic compound. In contrast, for DO, OX, and SB, a significant difference between the irradiated and non-irradiated groups could not be observed, and the in vivo phototoxicity of these 3 BZPs would be extremely low.

4. Discussion

In the present investigation, photosafety screening with the combined use of in vitro photochemical assessments and a dermal cassette-dosing approach was applied to in vivo phototoxicity evaluation on 6 dermally administered BZPs. From the present in vitro photochemical characterization, all BZPs indicated strong UV absorption on the basis of their MEC values (Henry et al., 2009); however, ROS generation from

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irradiated DO and SB was negligible. Referring to ICH S10 guideline, a negative result in the ROS assay supersedes a positive indication in UV/VIS spectral analysis

(ICH, 2014). BZ, KT, MX, and OX were found to have potent photoreactivity, possibly leading to phototoxic events in skin. By contrast, DO and SB were judged to be less phototoxic chemicals since ROS generation from irradiated DO and SB was negligible. Hence, the photoreactivity of BZPs was ranked on the basis of the present Downloaded from photochemical characterization as follows: KT＞BZ＞OX＞MX≫SB≒DO. The 3T3

NRU PT was also conducted, and, on the basis of the results obtained, the rank of in

vitro phototoxicity of BZPs was as follows: KT＞BZ≫OX≒SB≒DO. According to jpet.aspetjournals.org the dermal cassette-dosing PK study using UPLC/ESI-MS, skin exposure levels of KT and SB were higher and lasted longer than those of the other tested BZPs, and the order at ASPET Journals on September 24, 2021 for skin deposition properties on the basis of the Cmax and MRT was determined as follows: KT＞SB≫OX＞MX＞BZ＞DO.

The ICH S10 guideline (ICH, 2014) has described the critical characteristics of a phototoxicant as follows: (i) absorption of sunlight ranging from 290 to 700 nm; (ii) generation of reactive species including ROS; and (iii) sufficient distribution to light-exposed tissues. The in vitro photoreactivity/phototoxicity and in vivo PK profiles in the skin were obtained as crucial factors for evaluating the in vivo cutaneous phototoxic risk of tested BZPs. To integrate these results for phototoxic risk prediction on the 6 BZPs tested, a decision matrix was built using the experimental outcomes, including MEC, ROS data, PIF, Cmax, and MRT (Table 1). A decision matrix is a summarized schematic model of qualitative or quantitative data, which enables systemic identification, analysis, and evaluation of complicated sets of experimental outcomes

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(Seto et al., 2011). In the present decision matrix, if both in vitro photoreactivity/phototoxicity and PK profiles are high, the tested chemical is determined to have high phototoxic potential. On the other hand, a low level of either of these two factors would lead to a judgment of a mild phototoxic risk of the tested chemical. As weighting for the phototoxic prediction, in vitro photoreactivity/phototoxicity has precedence over in vivo PK profiles since Downloaded from photoreactivity is reported as a key trigger for phototoxic reactions (Onoue et al., 2009); therefore, low-photoreactive chemicals were determined to be less phototoxic even if

their PK parameters were high. ICH S10 guideline (ICH, 2014) has described that a jpet.aspetjournals.org photoreactive chemical with longer residence time in light-exposed tissues is more likely to produce a phototoxic reaction than a photoreactive compound having a shorter

residence time. On the basis of the decision matrix, KT was deduced to have the most at ASPET Journals on September 24, 2021 potent phototoxic risk because of its high in vitro photoreactivity/phototoxicity and high levels of skin deposition. Additionally, long-term dermal exposure risk of KT was suspected from the MRT value, and the cutaneous phototoxic risk might persist longer.

BZ would also have potent phototoxic risk owing to significant singlet oxygen generation and potent in vitro phototoxicity even though its skin deposition was low.

Although the in vitro phototoxicity of MX could not be evaluated by 3T3 NRU PT because of its poor solubility, MX determined to have phototoxic risk on the basis of its high photoreactivity and moderate skin exposure. On the other hand, OX was deduced to be less phototoxic agent due to the negative outcome from the in vitro phototoxicity test even if the positive results were obtained from in vitro photochemical assessments.

SB would have less phototoxic potential due to its low in vitro photoreactivity/phototoxicity although the MRT value of SB was almost the same as

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that of KT. DO was also determined to be less phototoxic since its low levels of both in vitro photoreactivity/phototoxicity and in vivo PK behaviors in the skin were observed. Overall, on the basis of the decision matrix, the order for in vivo phototoxic risk of tested BZPs was deduced as follows: KT≫BZ＞MX＞OX≫SB≒DO (Table 2).

To clarify the in vivo phototoxicity of tested BZPs, in vivo phototoxicity test was also conducted on the 6 BZPs. According to the phototoxic outcomes obtained Downloaded from from the experiment, the ranks of phototoxicity of BZPs were as follows: BZ＞KT≫

MX＞SB≒OX≒DO for in vivo phototoxicity (Table 2). From these findings, the order of in vivo phototoxic risk on 6 BZPs determined by photosafety screening was jpet.aspetjournals.org likely to show agreement with the in vivo phototoxicity of BZPs. As for MX, the 3T3

NRU PT was not applicable owing to its poorly aqueous solubility; however, the at ASPET Journals on September 24, 2021 photoreactivity of MX could be obtained using the ROS assay, and in vivo phototoxic risk of MX could be deduced on the basis of the photoreactivity and dermal PK behaviors. Thus, the photoreactivity data from the ROS assay would be of help for in vivo photosafety prediction if the in vitro phototoxicity information on a chemical is not available. Phototoxic skin reactions have been divided into at least three types, namely, photoirritancy, photogenotoxicity, and photoallergy, on the basis of the mechanisms of drug-induced phototoxicity, and ROS generation from an irradiated chemical can be a key trigger for all types of phototoxic events; therefore, the ROS assay is theoretically available for evaluating the risk of all types of phototoxicity (Onoue and Tsuda, 2006).

Hence, the proposed photosafety screening might be able to demonstrate all types of in vivo phototoxic risk of compounds with high accuracy, and, in the case of negative outcomes on chemicals, it would lead to the avoidance of in vivo phototoxicity test,

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achieving a reduction of the number of animals humanely killed.

For further investigation, Clog P values of BZPs were calculated using

ChemBioDraw Ultra 13.0 software as an indicator of their lipophilicity (Fig. 1) because lipophilicity has been thought to be a dominant factor determining the skin permeability of dermally administered chemicals (Abraham and Ibrahim, 2007). On the basis of the

Clog P values and the obtained concentration-time profiles in the skin, there appeared to Downloaded from be some relationship between lipophilicity and skin absorption behaviors of BZPs.

From these findings, lipophilicity and related parameters might be useful for estimating

the skin absorption properties of chemicals after dermal administration. By contrast, jpet.aspetjournals.org the prediction of the skin distribution of orally administered phenothiazines was challenging on the basis of Clog P values (Onoue et al., 2014a). Herein, for screening

purposes, the lipophilicity and related parameters might be of help to establish an in at ASPET Journals on September 24, 2021 silico approach as an alternative methodology for in vivo dermal PK study, and development of a non-animal photosafety assessment with combined use of in silico skin permeability and in vitro photoreactivity/phototoxicity might make a major contribution to animal welfare. However, in vivo PK profiling would still be important for estimating temporal changes in the phototoxic risk of compounds, including appearance time and duration of retention.

From the present findings, the proposed photosafety screening on the basis of the in vitro photoreactive/phototoxicity and in vivo PK data could evaluate the phototoxic risk of BZPs and would be applicable to the prediction of in vivo phototoxic risk, including all types of phototoxicity, on dermally administered chemicals with high accuracy. Recently, interest in the photosafety of chemicals has increased in both regulatory agencies and industry, and regulatory agencies have recommended the

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implementation of the 3Rs principle (refinement, reduction, and replacement).

Considering these trends surrounding product development, the proposed screening would be useful for evaluating the in vivo phototoxic risk of chemicals in product development on a large scale. Downloaded from jpet.aspetjournals.org at ASPET Journals on September 24, 2021

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Authorship Contribution

Participated in research design: Seto, Ohtake, Kato, Onoue

Conducted experiments: Ohtake, Kato

Contributed new regents or analytic tools: Seto, Ohtake, Kato, Onoue

Performed data analysis: Seto, Ohtake, Kato, Onoue

Wrote or contributed to the writing of the manuscript: Seto, Ohtake, Onoue Downloaded from jpet.aspetjournals.org at ASPET Journals on September 24, 2021

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Footnotes

This work was supported in part by a Health Labour Sciences Research Grant from The Ministry of Health, Labour and Welfare, Japan [H25-iyaku-wakate-024]; a grant from the Cosmetology Research Foundation [No. 527]; and a grant from the Hoyu

Science Foundation [No. 31].

Downloaded from jpet.aspetjournals.org at ASPET Journals on September 24, 2021

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Figure Legends

Fig. 1 Chemical structure of tested BZPs. a) Calculated using ChemBioDraw Ultra

13.0 software.

Fig. 2 In vitro photochemical properties of BZPs. (A) UV absorption spectra of

BZPs (20 μM) in 20 mM NaPB (pH7.4). Thick solid line, BZ; thin solid line, KT; thin Downloaded from dashed line, OX; thick dashed line, SB; thin dotted line, DO; and thick dotted line, MX.

(B) Generation of singlet oxygen (filled bars) and superoxide (open bars) from BZPs

2 (200 μM) exposed to simulated sunlight (ca. 2.0 mW/cm ). QN and EM as positive jpet.aspetjournals.org and negative controls, respectively. Data represent mean±SD for three experiments.

N.D., not detected; and N.A., not applicable.

at ASPET Journals on September 24, 2021

Fig. 3 Skin concentration-time profiles after dermal co-administration of BZPs (0.1 mg/rat each). ■, BZ; △, KT; ▽, OX; □, SB; ○, DO; and ◇, MX. Data represent mean for five rats.

Fig. 4 Colorimetrical evaluation of skin inflammation induced by irradiated BZPs.

Differences in skin color (ΔE) between before and after irradiation were estimated on the basis of L*, a*, and b* values. QN and EM as positive and negative controls, respectively. Filled bars, UVA-irradiated group; and open bars, non-irradiated group.

Data represent mean±SE of four to five rats. #p＜0.05, significantly different from corresponding non-irradiated group.

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Table 1 Decision matrix This articlehasnotbeencopyeditedandformatted.Thefinalversionmaydifferfromthisversion. BZ DO KT MX OX SB UV/VIS spectral analysis JPET FastForward.PublishedonMay27,2015asDOI:10.1124/jpet.115.223644 MEC (M-1・cm-1) 2,317 10,833 4,350 2,850 6,817 11,700 ROS assay

1 3 a) c) O2 (ΔA440 nm・10 ) 219 N.D. 318 67 147 N.D.

- 3 b) c) O2 (ΔA560 nm・10 ) N.D. N.D. 94 N.D. N.D. N.D. 3T3 NRU PT PIF 49.5 1.0 68.9 N.A. 1.0 1.0 PK analysis after dermal co-administrationd)

Tmax (h) 4 6 24 8 6 24

Cmax (μg/g tissue) 6.1 ± 0.6 6.0 ± 1.0 8.7 ±0.9 7.8 ± 0.5 7.8 ± 1.2 7.8 ±0.5

AUC0–24 (h・μg/g tissue) 64.5 ± 2.8 53.4 ± 3.1 160.0 ±7.3 87.6 ± 4.0 98.6 ± 5.1 133.5 ±7.4

MRT (h) 8.4 ± 0.3 7.2 ± 0.3 ＞14.2 8.4 ±0.2 9.3 ± 0.2 ＞14.9 Black cells represent high levels of each crucial factor: MEC values over 1,000 M-1・cm-1 in the UV/VIS spectral analysis; ROS data

1 3 - 3 over criteria in the ROS assay ( O2 [ΔA440 nm・10 ]: 25; and O2 [ΔA560 nm・10 ]: 20); PIF values over criteria in the 3T3 NRU PT (PIF: 5);

33

Downloaded from from Downloaded jpet.aspetjournals.org at ASPET Journals on September 24, 2021 24, September on Journals ASPET at JPET #223644

and higher values of PK parameter (Cmax and MRT) in the PK analysis. N.D.: not detected; and N.A.: not applicable due to solubility This articlehasnotbeencopyeditedandformatted.Thefinalversionmaydifferfromthisversion. 3 3 issue. a) Decrease in A440 nm・10 . b) Increase in A560 nm・10 . c) ROS data from irradiated MX (100 μM). d) Cmax and MRT values JPET FastForward.PublishedonMay27,2015asDOI:10.1124/jpet.115.223644 were used as crucial factors for in vivo phototoxic prediction. Each value represents mean±SE for five rats.

34

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Table 2 The outcomes from photosafety assessments on BZPs

Obtained data/Prediction This articlehasnotbeencopyeditedandformatted.Thefinalversionmaydifferfromthisversion. Proposed photosafety screenings KT ≫ BZ ＞ MX ＞ OX ≫ SB ≒ DO JPET FastForward.PublishedonMay27,2015asDOI:10.1124/jpet.115.223644 In vivo phototoxicity test BZ ＞ KT ＞ MX ≫ SB ≒ OX ≒ DO

35

Downloaded from from Downloaded jpet.aspetjournals.org at ASPET Journals on September 24, 2021 24, September on Journals ASPET at JPET Fast Forward. Published on May 27, 2015 as DOI: 10.1124/jpet.115.223644 This article has not been copyedited and formatted. The final version may differ from this version.

BZPs CAS No. ClogP a) R1 R2 R3 R4 R5 R6 Downloaded from

Benzophenone (BZ) 119-61-9 3.18 –H –H –H –H –H –H

Dioxybenzone (DO) 131-53-3 3.49 –OH –H –OCH3 –H –OH –H jpet.aspetjournals.org

Ketoprofen (KT) 22071-15-4 2.76 –H –H –H –H –H

Mexenone (MX) 1641-17-4 4.08 –OH –H –OCH3 –H –H –CH3

Oxybenzone (OX) 131-57-7 3.59 –OH –H –OCH3 –H –H –H at ASPET Journals on September 24, 2021

Sulisobenzone (SB) 4065-45-6 0.80 –OH –H –OCH3 –SO3H–H–H

Fig. 1 JPET Fast Forward. Published on May 27, 2015 as DOI: 10.1124/jpet.115.223644 This article(A) has not been copyedited and formatted. The final version may differ from this version.

UVC UVB UVA 0.3

0.2 Absorbance 0.1

0.0 240 260 280 300 320 340 360 380 400 Wavelength (nm) Downloaded from

(B) 250 400 ) ) 3 3

10 200 . 10 . jpet.aspetjournals.org 300 150 440 nm 560 nm

200 100 Superoxide Singlet oxygen Singlet

100 50 (Increase of A (Decrease of A at ASPET Journals on September 24, 2021

N.D. N.D. N.A . N.D. N.D. N.D. 0 0 BZ DO KT MX OX SB QN EM

Fig. 2 JPET Fast Forward. Published on May 27, 2015 as DOI: 10.1124/jpet.115.223644 This article has not been copyedited and formatted. The final version may differ from this version.

10.0

7.5 g/g tissue) 

5.0

2.5 Skin concentration ( 0.0 0 4 8 12 24

Time (h) Downloaded from jpet.aspetjournals.org at ASPET Journals on September 24, 2021

Fig. 3 JPET Fast Forward. Published on May 27, 2015 as DOI: 10.1124/jpet.115.223644 This article has not been copyedited and formatted. The final version may differ from this version. #

15 #

E ) # 

10

5 Change in skin color (

0 BZ DO KT MX OX SB QN EM Downloaded from jpet.aspetjournals.org at ASPET Journals on September 24, 2021

Fig. 4