Monoclonal Antibody to Podocalyxin / PODXL - Purified

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AM32857PU-S Monoclonal Antibody to Podocalyxin / PODXL - Purified

Alternate names: GCTM-2 antigen, Hematopoietic Stem Cell Marker, PCLP, PCLP-1, PCLP1, Podocalyxin- like protein 1 Quantity: 0.1 mg Concentration: 0.2 mg/ml Background: Podocalyxin, also known as podocalyxin-like or PDXL, is a member of the CD34 transmembrane sialomucin family. Like other sialoglycoproteins, podocalyxin plays important roles in mediating cellular migration and cell-cell adhesion. Podocalyxin was first discovered in podocytes, the terminally differentiated epithelial cells lining the kidney glomeruli. Podocalyxin is expressed at high levels on the podocyte foot projections and plays essential roles in kidney development and homeostasis, particularly blood filtration and urine formation. Podocalyxin is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons (reviewed in Nielsen, 2009). Aberrant expression of podocalyxin has been described in a variety of cancers including breast, prostate, liver, pancreatic, kidney and leukemia. Human podocalyxin migrates as a 165/170 kDa doublet on SDS-PAGE; however there are many post-translational modifications which may impact its actual size (Kershaw, 1997). Antibody to podocalyxin has been reported to be a useful biomarker for various pathological conditions. For example, podocalyxin antibody can identify podocytes in the urine, also known as podocyturia, which can be indicative of glomerular disease, pre-eclampsia, and other kidney pathology (reviewed in Chen, 2012). Additionally, podocalyxin antibody studies have indicated that highly podocalyxin-positive tumors or podocalyxin dysregulation in general can be potentially linked to more aggressive tumors and poorer outcomes (Nielson, 2009). Hence, podocalyxin antibody may be used as part of a biomarker antibody panel to study expression of proteins associated with tumorigenesis. Uniprot ID: O00592 NCBI: NP_001018121.1 GeneID: 5420 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), SM20P (for use in rat samples), AM03095PU-N Controls: Clone: 3D3 Immunogen: Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of Human Podocalyxin.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. RS/20150318 1 / 3 AM32857PU-S: Monoclonal Antibody to Podocalyxin / PODXL - Purified

Format: State: Liquid purified IgG fraction from Bioreactor Concentrate Purification: Protein A/G Chromatography Buffer System: 10mM PBS Preservatives: 0.05% Sodium Azide Stabilizers: 0.05% BSA Applications: ELISA: Use Antibody without BSA for Coating. Western Blot: 0.5-1.0 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein. Flow Cytometry: 0.5-1 µg/106 cells. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: 165-170kDa Specificity: The 3D3 antibody recognizes an epitope in the extracellular domain, and both the 4F10 and 2A4 antibody clones recognize epitopes in the intracellular domain of Podocalyxin. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. The Podocalyxin antibody Clones 3D3 (Cat.-No AM32857PU), 4F10 and 2A4 have been extensively published in the literature and researchers are encouraged to consult the published literature for additional information. Cellular Localization: Cell surface and cytoplasmic. Species Reactivity: Tested: Human, Rabbit, Rat. Storage: Store undiluted at 2-8°C. Shelf life: one year from despatch. General Readings: 1. Kershaw DB, Beck SG, Wharram BL, Wiggins JE, Goyal M, Thomas PE, et al. Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J Biol Chem. 1997 Jun 20;272(25):15708-14. PubMed PMID: 9188463. 2. Sassetti C, Tangemann K, Singer MS, Kershaw DB, Rosen SD. Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34. J Exp Med. 1998 Jun 15;187(12):1965-75. PubMed PMID: 9625756. 3. Schopperle WM, Kershaw DB, DeWolf WC. Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin. Biochem Biophys Res Commun. 2003 Jan 10;300(2):285-90. PubMed PMID: 12504081. 4. Chen et al. Reproductive Sciences (2012) DOI:10.1177/1933710112459227. 5. Nielsen JS, McNagny KM. The role of podocalyxin in health and disease. J Am Soc Nephrol. 2009 Aug;20(8):1669-76. doi: 10.1681/ASN.2008070782. Epub 2009 Jul 2. PubMed PMID: 19578008.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. RS/20150318 2 / 3 AM32857PU-S: Monoclonal Antibody to Podocalyxin / PODXL - Purified

Pictures: Formalin-Fixed, Paraffin-Embedded Human angiosarcoma stained with Podocalyxin Antibody Cat.-No AM32857PU (Clone 3D3).

Western blot analysis of HeLa lysate probed with Podocalyxin Antibody (Clone 3D3).

Surface staining of NCCIT cells using Podocalyxin Antibody (Clone 3D3).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. RS/20150318 3 / 3