SANTA CRUZ BIOTECHNOLOGY, INC. Amelogenin (N-20): sc-33121

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BACKGROUND PRODUCT Dental enamel is a highly mineralized tissue with most of its volume occupied Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide by large, highly organized, hydroxyapatite crystals. This structure is thought and 0.1% gelatin. to be controlled through the interaction of many organic matrix molecules Blocking peptide available for competition studies, sc-33121 P, (100 µg including amelogenin, , , and several other peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). enzymes. All of these secreted proteins are involved in the mineralization and enamel matrix formation in developing . The AMELX, APPLICATIONS which encodes for the protein Amelogenin, is encoded on the X-chromosome. Amelogenin, also designated AMG, AMGX or AMEX, is involved in biomin- Amelogenin (N-20) is recommended for detection of Amelogenin of mouse, eralization and organization of developing enamel. It functions by regulating rat and human origin by Western Blotting (starting dilution 1:200, dilution crystallite formation during the secretory stage of enamel development. range 1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of total Amelogenin, which localizes to the extracellular matrix, is expressed by protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, and is the predominant protein in developing dental enamel. dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, Defects in the AMELX gene can cause imperfecta hypoplastic dilution range 1:30-1:3000). type 1 (AIH1) which is an X-linked disease that affects the formation of tooth Suitable for use as control antibody for Amelogenin siRNA (h): sc-44845 enamel. and Amelogenin siRNA (m): sc-44846.

REFERENCES Molecular Weight of Amelogenin: 24 kDa. 1. Nakahori, Y., et al. 1991. A human X-Y homologous region encodes Positive Controls: NIH/3T3 whole cell lysate: sc-2210 or Mouse embryo “amelogenin”. Genomics 9: 264-269. tissue extract.

2. Catalano-Sherman, J., et al. 1993. Amino acid sequence of a major human RECOMMENDED SECONDARY REAGENTS amelogenin protein employing Edman degradation and cDNA sequencing. J. Dent. Res. 72: 1566-1572. To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 3. Catalano-Sherman, J., et al. 1994. Production of a monoclonal antibody (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey against human Amelogenin. Calcif. Tissue Int. 54: 76-80. anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ 4. Lagerstrom-Fermer, M., et al. 1995. Amelogenin signal peptide mutation: Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: correlation between mutations in the Amelogenin gene (AMGX) and mani- sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- festations of X-linked . Genomics 26: 159-162. cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution 5. Du, C., et al. 2005. Apatite/Amelogenin coating on titanium promotes range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: osteogenic gene expression. J. Dent. Res. 84: 1070-1074. 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. 6. Habelitz, S., et al. 2005. Amelogenin control over apatite crystal growth is affected by the pH and degree of ionic saturation. Orthod. Craniofac. Res. 8: DATA 232-238. 132 K 7. Foster, B.L., et al. 2005. Regenerating the periodontium: is there a magic 90 K formula? Orthod. Craniofac. Res. 8: 285-291. 55 K 43 K 8. Torres-Quintana, M.A., et al. 2005. Ameloblastin and Amelogenin expres- sion in posnatal developing mouse molars. J. Oral. Sci. 47: 27-34. 34 K

< Amelogenin CHROMOSOMAL LOCATION 23 K Genetic locus: AMELX (human) mapping to Xp22.31-p22.1; Amelx (mouse) Amelogenin (N-20): sc-33121. Western blot analysis of mapping to X F5. Amelogenin expression in NIH/3T3 whole cell lysate.

SOURCE STORAGE Amelogenin (N-20) is an affinity purified goat polyclonal antibody raised Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of against a peptide mapping at the N-terminus of Amelogenin of human origin. shipment. Non-hazardous. No MSDS required.

RESEARCH USE PROTOCOLS For research use only, not for use in diagnostic procedures. See our web site at www.scbt.com or our catalog for detailed protocols and support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com