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Rabbit Anti-DNA alpha/FITC Conjugated antibody

SL6166R-FITC

Product Name: Anti-DNA polymerase alpha/FITC Chinese Name: FITC标记的DNA聚合酶α抗体 DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; Alias: DPOLA_HUMAN; P180; p180 subunit; POLA; POLA1; Polymerase (DNA directed), alpha 1, catalytic subunit; Polymerase (DNA-directed), alpha (70kD). Organism Species: Rabbit Clonality: Polyclonal React Species: Human,Mouse,Rat,Dog,Horse,Rabbit, IF=1:50-200 Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 161kDa Form: Lyophilized or Liquid Concentration: 1mg/ml KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase immunogen: www.sunlongbiotech.com alpha Lsotype: IgG Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year Storage: when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. background: Plays an essential role in the initiation of DNA replication. During the S phase of the Product Detail: cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Function: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Subunit: The DNA polymerase alpha complex is composed of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. Interacts with PARP1; this interaction functions as part of the control of replication fork progression. Interacts with MCM10 and WDHD1; these interactions recruit the polymerase alpha complex to the pre- replicative complex bound to DNA. Interacts with RPA1; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interacts with SV40 Large T antigen; this interaction allows viralwww.sunlongbiotech.com DNA replication. Interacts with herpes simplex virus 1 (HHV-1) replication origin-binding protein UL9.

Subcellular Location: Nucleus. [PTM] A 165 kDa form is probably produced by proteolytic cleavage at Lys- 124.

Similarity: Belongs to the DNA polymerase type-B family.

Database links:

Entrez : 5422Human Entrez Gene: 18968Mouse

Entrez Gene: 85241Rat

Omim: 312040Human

SwissProt: P09884Human

SwissProt: P33609Mouse

SwissProt: O89042Rat

Unigene: 567319Human

Unigene: 1923Mouse

Unigene: 187125Rat

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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