Glycogen Synthase Kinase 3 beta (GSK3b)

Catalog number G8170-44F Supplier United States Biological

GSK3 beta, also known as Tpk1 (tau protein kinase-1), is a 47kD protein-serine/threonine kinase with broad specificity that phosphorylates and inactivates glycogen synthase, the phosphatase 1 inhibitor protein, and other proteins that feature a (S/T)PXX(Tp/Sp) recognition motif. It is ubiquitously distributed, but highly expressed in mammalian brain, and occurs in two isoforms, 51kD (alpha) and 47kD (beta). This monomeric proline-directed kinase is inhibited when it is phosphorylated by protein kinase B and S6 protein kinases. It is activated when it is phosphorylated by an unknown protein- tyrosine kinase. GSK3beta is inhibited in response to insulin. GSK3beta is phosphorylated and inactivated by protein kinase C, p70, S6 kinase and Rsk-1.

Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions Western Blot (Colorimetric): 1:1000 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by researcher.

Positive Controls: HeLa Cell Lysate, Mouse Brain Tissue Extract Rat Brain Tissue Extract Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunogen Synthetic peptide corresponding to aa335-349, CAHSFFDELRDPNVK from rat GSK3b, conjugated to KLH. Formulation Supplied as a liquid PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Purity Purified by Protein A affinity chromatography from serum Specificity Recognizes rat GSK3b at ~47kD. Species Crossreactivity: human, mouse and bovine Product Type Pab Source rat Isotype IgG Grade Affinity Purified Applications IC IHC WB Crossreactivity Bo Hu Mo Rt Storage -20°C Reference 1. Woodgett, J.R. (1990) EMBO J. 9: 2431-2438. 2. Behrens, J., Jerchow, B.-A., Würtele, M., Grimm, J., Asbrand, C., Wirtz, R., Kühl, M., Wedlich, D. and Birchmeier, W. (1998) Science 280: 596-599. 3. Ishiguro, K. et al (1993) FEBS Lett. 325: 167-172. 4. Hardie, G. and Hanks, S. (1995) The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps

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