Anti-RIC8A Mouse mAb

Catalog # PTM-5768

General Information Images

Host species Mouse WB

Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1432 Primary ab dilution: 1:1000 Synonym / Primary ab incubation condition: 2 hours at room temperature Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Rabbit IgG H&L species (HRP) Human Q9NPQ8 65 WB Lysate: HeLa, 293, Raji, A431 loading quantity: 20 μg Product Usage Information Exposure time: 3 s Predicted MW: 65 kDa Application Dilution Recommended species Observed MW: 65 kDa WB 1:500-1:1000 Human Research Use

Properties For research use only, not for use in diagnostic procedures.

Storage Store at -20°C. Avoid freeze / thaw cycles.

Stability Stable for 12 months from date of receipt / reconstitution

Constituents PBS, Glycerol, BSA

Purity Protein G purified

Isotype IgG1/Kappa

Target Information

Function Guanine nucleotide exchange factor (GEF), which can

activate some, but not all, G-alpha . Able to

activate GNAI1, GNAO1 and GNAQ, but not GNAS by

exchanging bound GDP for free GTP. Involved in

regulation of microtubule pulling forces during mitotic

movement of by stimulating G(i)-alpha

protein, possibly leading to release G(i)-alpha-GTP and

NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP

complex (By similarity). Also acts as an activator for G(q)-

alpha (GNAQ) protein by enhancing the G(q)-coupled

receptor-mediated ERK activation.

Cellular location Membrane, Cytoplasm

APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com