Anti-RIC8A Mouse mAb
Catalog # PTM-5768
General Information Images
Host species Mouse WB
Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1432 Primary ab dilution: 1:1000 Synonym / Primary ab incubation condition: 2 hours at room temperature Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Rabbit IgG H&L species (HRP) Human Q9NPQ8 65 WB Lysate: HeLa, 293, Raji, A431 Protein loading quantity: 20 μg Product Usage Information Exposure time: 3 s Predicted MW: 65 kDa Application Dilution Recommended species Observed MW: 65 kDa WB 1:500-1:1000 Human Research Use
Properties For research use only, not for use in diagnostic procedures.
Storage Store at -20°C. Avoid freeze / thaw cycles.
Stability Stable for 12 months from date of receipt / reconstitution
Constituents PBS, Glycerol, BSA
Purity Protein G purified
Isotype IgG1/Kappa
Target Information
Function Guanine nucleotide exchange factor (GEF), which can
activate some, but not all, G-alpha proteins. Able to
activate GNAI1, GNAO1 and GNAQ, but not GNAS by
exchanging bound GDP for free GTP. Involved in
regulation of microtubule pulling forces during mitotic
movement of chromosomes by stimulating G(i)-alpha
protein, possibly leading to release G(i)-alpha-GTP and
NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP
complex (By similarity). Also acts as an activator for G(q)-
alpha (GNAQ) protein by enhancing the G(q)-coupled
receptor-mediated ERK activation.
Cellular location Membrane, Cytoplasm
APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com