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XXI International Grassland Congress / VIII International Grassland Congress Proceedings International Rangeland Congress

Comparison of Natural and Cultivated Species from Mongolian Rangelands and Their DNA Sequences

S. Lkhagvasuren Mongolian Academy of Sciences,

L. Enkhtuya Mongolian Academy of Sciences, Mongolia

Ts. Munkhzul Mongolian Academy of Sciences, Mongolia

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Part of the Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky.edu/igc/21/13-1/43 The XXI International Grassland Congress / VIII International Rangeland Congress took place in Hohhot, from June 29 through July 5, 2008. Proceedings edited by Organizing Committee of 2008 IGC/IRC Conference Published by Guangdong People's Publishing House

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Comparison of natural and cultivated Iris species from Mongolian rangelands and their DNA sequences

S . Lkhagv asuren , L . Enkhtuya , Ts . Munkhz ul Institute o f Botany , Mongolian A cademy o f Sciences , Ulaanbaatar‐210351 , Mongolia Lsuren52＠ yahoo .com

Key words : ,Iris dichotoma physiology ,transpiration rate Introduction ３０００ ８００ The flora of Mongolia includes１a９t８２leastIris species of vascular , and out of these approximately have been used for medicinal purposes ( Grubov , ) . species have been frequently used fo２r３the treatment of various diseases , such as bacterial infections , cancer and inflammation . From２００th２e substances １in２volved , comIrpisounds have been th‐us far succes‐sfully isolated and‐their identity revealed ( Purev et .al . , ) . A total of collections of species from deIsreisrt Gobi , steppe grassland , forest steppe , and mountains were２０p０la４nted at the Botanical Garden in Ulaanbaatar , Mongolia . . species are an important medicinal plant group ( Enkhtuya２,００４ ) . Phylogenetic relationship trees are useful for predicting medicinal compounds in other species ( Lkhagvasuren et al . , ) . DNA sequence data of this species have been submitted to Genbank . Materials and methods １９５０ Water content , water deficit , and transpiration rate were determined by the methods of Ivanov et al . . Total plant genomic DNA was extracted with DNeasy Plant Mini Kit ( Qiagen , Hilden , Germany) . PCR amplification ( ) rp l16 rpl14 ５摧‐ of the plastid DNA fragment between and ‐３摧 genes was perform５e摧d‐ with the sense primer AAAGATCTAGAT T TCG‐T３摧AAACAACATAGAGGAAGAA and antisense primer ATCTGCAGCAT T TAAAAGGG TCTGAGGT T３G７３AATCAT . Sequencing was performed using d‐ye termination sequencing protocol . The reactions were analysed on a A Fluorescent Automated Sequencer ( Applied Bio systems , Perkin Elmer) . Results and discussion Iris dichotoma Collectio７n７s５of ８１ ８％ from the grassland st１e３ppe １w５e％re cultivated at the Botanical Garden . ThIreiys rdaincgheodtominawater content from . to ０ .４６ and had a water def４ici３t from to . The average rate of transpiration for in the grassland steppe was . g / g /Ihricsodmicphaorteodmtao . g/ g / h in the Botanical Garden . Curves were developed for the daily and seasonal course of transpiration of growing in the Botanical Garden and the grassland steppe . Conclusions Iris dichotoma ９ ３ Iris dichotoma The rate of transpiration of grown in the Botanical Garden was . times greater than in the grasslan１d９steppe . Plants growing in the Botanical Garden had several times greater numbers of reproductIirvies sdhicohootstompear plant and had times more flowers per p‐lant than plants from the grassland steppe . DIrNisAdiscehqoutoemncae data of and Iris AbBunOge7i73h6av5e) been submitted to Gen bank . ( Lkhagvasuren S . DNA sequence of Pall . , DNA data bank number References １９５０ Ivanov , L .A .B,oStil.inJaou,rAna.Al ３.５, T２seln１ik７e１r‐１,８U５ .L . , . The methods for determination transpiration rates by weight in natural conditions . ( ) , . ２００４ Lkhagvasuren , S . , Hampp , R . , Tarkka , M . , Enkhtuya , L . , Sanchir , Ch . , Zumberelmaa , D . , . Phylogenetic Iris Proceeding Institute o f Botany , MA S . １５ １３５‐１４２ relationship of species based on DNA sequences . ２,００２ . Purev , O . , Purevsuren , Cs . , Narantuya , S . , Lkhagvasuren , S . , Mizukami , H . , Nagatsu , A . , . New isoflavones and Iris potaninii , Bull , ５０ １０ １３６７‐１３６９ Flavanol from Chem . Pharm . , . ２００４ Iris species . Proc(eed)ings o f Institute o f Botany , MA S １５ １９２ Enkhtuya , L . , . The collection of , .

Grasslands/Rangelands Production Systems——— Domestication of Native Grasslands/Rangelands Plants for Regional Use