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Design of Nucleic Acid Strands with Long Low-Barrier Folding Pathways
Nat Comput DOI 10.1007/s11047-016-9587-9 Design of nucleic acid strands with long low-barrier folding pathways Anne Condon1 · Bonnie Kirkpatrick2 · Ján Maˇnuch1 © The Author(s) 2017. This article is published with open access at Springerlink.com Abstract A major goal of natural computing is to design fully developed, that exploit base pairing interactions of biomolecules, such as nucleic acid sequences, that can be nucleic acids. Prominent examples include DNA strand dis- used to perform computations. We design sequences of placement systems (DSDs) (Seelig et al. 2006) and RNA nucleic acids that are “guaranteed” to have long folding origami systems (Geary and Andersen 2014). Our work here pathways relative to their length. This particular sequences is motivated by the goal of computing with a single RNA with high probability follow low-barrier folding pathways sequence as the nucleic acids of the sequence interact with that visit a large number of distinct structures. Long fold- each other. ing pathways are interesting, because they demonstrate that RNA sequences form folded structures in which pairs of natural computing can potentially support long and com- nucleic acids biochemically bond to each other. These bonds plex computations. Formally, we provide the first scalable change the physical energy of the sequence, and a given designs of molecules whose low-barrier folding pathways, sequence prefers to assume low-energy folded structures. with respect to a simple, stacked pair energy model, grow Folding is a dynamic process, constrained by kinetics, dur- superlinearly with the molecule length, but for which all sig- ing which an RNA sequence will move through a sequence nificantly shorter alternative folding pathways have an energy of structures with each differing from the previous one by barrier that is 2 − times that of the low-barrier pathway for the addition or removal of a single base pair. -
Still Photography
Still Photography Soumik Mitra, Published by - Jharkhand Rai University Subject: STILL PHOTOGRAPHY Credits: 4 SYLLABUS Introduction to Photography Beginning of Photography; People who shaped up Photography. Camera; Lenses & Accessories - I What a Camera; Types of Camera; TLR; APS & Digital Cameras; Single-Lens Reflex Cameras. Camera; Lenses & Accessories - II Photographic Lenses; Using Different Lenses; Filters. Exposure & Light Understanding Exposure; Exposure in Practical Use. Photogram Introduction; Making Photogram. Darkroom Practice Introduction to Basic Printing; Photographic Papers; Chemicals for Printing. Suggested Readings: 1. Still Photography: the Problematic Model, Lew Thomas, Peter D'Agostino, NFS Press. 2. Images of Information: Still Photography in the Social Sciences, Jon Wagner, 3. Photographic Tools for Teachers: Still Photography, Roy A. Frye. Introduction to Photography STILL PHOTOGRAPHY Course Descriptions The department of Photography at the IFT offers a provocative and experimental curriculum in the setting of a large, diversified university. As one of the pioneers programs of graduate and undergraduate study in photography in the India , we aim at providing the best to our students to help them relate practical studies in art & craft in professional context. The Photography program combines the teaching of craft, history, and contemporary ideas with the critical examination of conventional forms of art making. The curriculum at IFT is designed to give students the technical training and aesthetic awareness to develop a strong individual expression as an artist. The faculty represents a broad range of interests and aesthetics, with course offerings often reflecting their individual passions and concerns. In this fundamental course, students will identify basic photographic tools and their intended purposes, including the proper use of various camera systems, light meters and film selection. -
Algorithms for Nucleic Acid Sequence Design
Algorithms for Nucleic Acid Sequence Design Thesis by Joseph N. Zadeh In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy California Institute of Technology Pasadena, California 2010 (Defended December 8, 2009) ii © 2010 Joseph N. Zadeh All Rights Reserved iii Acknowledgements First and foremost, I thank Professor Niles Pierce for his mentorship and dedication to this work. He always goes to great lengths to make time for each member of his research group and ensures we have the best resources available. Professor Pierce has fostered a creative environment of learning, discussion, and curiosity with a particular emphasis on quality. I am grateful for the tremendously positive influence he has had on my life. I am fortunate to have had access to Professor Erik Winfree and his group. They have been very helpful in pushing the limits of our software and providing fun test cases. I am also honored to have two other distinguished researchers on my thesis committee: Stephen Mayo and Paul Rothemund. All of the work presented in this thesis is the result of collaboration with extremely talented individuals. Brian Wolfe and I codeveloped the multiobjective design algorithm (Chapter 3). Brian has also been instru- mental in finessing details of the single-complex algorithm (Chapter 2) and contributing to the parallelization of NUPACK’s core routines. I would also like to thank Conrad Steenberg, the NUPACK software engineer (Chapter 4), who has significantly improved the performance of the site and developed robust secondary structure drawing code. Another codeveloper on NUPACK, Justin Bois, has been a good friend, mentor, and reliable coding partner. -
How All This Came About
Innovation. Amplified. Chapter 1 How All This Came About by Hartley Peavey s most of you know, electronics have been direction (i.e. a “check valve” that allows electrons to A around a very long time. In the latter part of the flow in only one direction). It had been long known 1800s, Thomas Edison perfected the incandescent that electrons possess a “negative” (-) charge and lightbulb. Edison experimented with thousands of therefore are attracted to anything having a positive combinations of materials before he finally found (+) charge. So the flow of electrons is (and will al- that a small Tungsten filament inside an “evacuated” ways be) from negative to positive. glass container would convert electricity into light. These early bulbs suffered a number of problems, The aforementioned “Edison effect” became widely but generally were perfected enough for general known, and various labs on both sides of the Atlantic use by the early 1890s... After extended use, it was performed extensive research. The modern vacuum discovered that the inside of the clear glass “bulbs” tube utilizes three or more “electrodes” whose effect would gradually darken, thus absorbing much of was discovered in 1903 by an American named Lee the light generated by the incandescent filament. DeForrest. He discovered that if an electrode with Various schemes were tried to reduce this, includ- a negative charge was inserted between the incan- ing introduction of various “Noble” gases, as well descent filament and a positively charged electrode as insertion of other metal conductors in attempts (anode), that the flow of current could be controlled to “drain off” whatever was causing the inside of (modulated), thus causing the device to act like an Edison’s bulbs to blacken after extended periods of “electronic valve”… This is why most of the world use. -
|||GET||| the Design-Build Studio 1St Edition
THE DESIGN-BUILD STUDIO 1ST EDITION DOWNLOAD FREE Tolya Stonorov | 9781317307969 | | | | | Welcome to the DBXchange Platform A study from the US Department of Transportation claims that: "Design-build delivery has been steadily increasing in the U. Untilthe AIA American Institute of Architects ' code of ethics and professional conduct prohibited their members from providing construction services. Building performance is measured early too, so that trade offs between budget, schedule, functionality and usability can inform specification and continuous refinement of the design. With the petition "Macht mehr DesignBuild" we would like to approach the colleges and universities to point out the importance of DesignBuild in teaching future architects and civil engineers. But year-over-year multifamily trendline remained negative at The design-build contractor is responsible for all work on the project, so the client can seek legal remedies for any fault from one party. Big Room equals big results. The Weekly Show: AI for building facade inspections; designing a world-class architecture firm. In presenting these case studies from the U. Standard contract document for prefab and modular building released. The Design-Build Studio 1st edition publication gives guidance through the different phases of the process: design services, contracts, management, insurances, and finances. More information HERE. With the The Design-Build Studio 1st edition of the Burdette Keeland, Jr. Retrieved Industrial architecture Interior architecture Landscape architecture Vernacular architecture. The "design—builder" is often a general contractorbut in many cases a project is led by a design professional architectengineerarchitectural technologist or other professional designers. Construction Industry Institute Research Report : — Restricted Content You must have JavaScript enabled to enjoy a limited number of articles over the next 30 days. -
A Global Sampling Approach to Designing and Reengineering RNA
i “rnaensign” — 2017/7/1 — 22:02 — page 1 — #1 i i i Published online ?? Nucleic Acids Research, ??, Vol. ??, No. ?? 1–11 doi:10.1093/nar/gkn000 Aglobalsamplingapproachtodesigningandreengineering RNA secondary structures 1,2, 1, 3 1 1 Alex Levin ⇤,MieszkoLis ⇤,YannPonty ,CharlesW.O’Donnell ,SrinivasDevadas , 1,2, 1,2,4, Bonnie Berger †,J´erˆomeWaldisp¨uhl † 1 Computer Science and Artificial Intelligence Laboratory, MIT, Cambridge, MA 02139, USA. and 2 Department of Mathematics, MIT, Cambridge, MA 02139, USA. and 3 Laboratoire d’Informatique, Ecole´ Polytechnique, Palaiseau, France. and 4 School of Computer Science, McGill University, Montreal, QC H2A 3A7, Canada. Received ??; Revised ??; Accepted ?? ABSTRACT mechanisms (1), reprogramming cellular behavior (2), and designing logic circuits (3). The development of algorithms for designing artificial Here, we aim to design RNA sequences that fold into RNA sequences that fold into specific secondary structures specific secondary structures (a.k.a. inverse folding). Even has many potential biomedical and synthetic biology in this case, efficient computational formulations remain applications. To date, this problem remains computationally difficult, with no exact solutions known. Instead, the solutions difficult, and current strategies to address it resort available today rely on local search strategies and heuristics. to heuristics and stochastic search techniques. The Indeed, the computational difficulty of the RNA design most popular methods consist of two steps: First a problem was proven by M. Schnall-Levin et al. (4). random seed sequence is generated; next, this seed is One of the first and most widely known programs for progressively modified (i.e. mutated) to adopt the desired the RNA inverse folding problem is RNAinverse (5). -
California Noise: Tinkering with Hardcore and Heavy Metal in Southern California Steve Waksman
ABSTRACT Tinkering has long figured prominently in the history of the electric guitar. During the late 1970s and early 1980s, two guitarists based in the burgeoning Southern California hard rock scene adapted technological tinkering to their musical endeavors. Edward Van Halen, lead guitarist for Van Halen, became the most celebrated rock guitar virtuoso of the 1980s, but was just as noted amongst guitar aficionados for his tinkering with the electric guitar, designing his own instruments out of the remains of guitars that he had dismembered in his own workshop. Greg Ginn, guitarist for Black Flag, ran his own amateur radio supply shop before forming the band, and named his noted independent record label, SST, after the solid state transistors that he used in his own tinkering. This paper explores the ways in which music-based tinkering played a part in the construction of virtuosity around the figure of Van Halen, and the definition of artistic ‘independence’ for the more confrontational Black Flag. It further posits that tinkering in popular music cuts across musical genres, and joins music to broader cultural currents around technology, such as technological enthusiasm, the do-it-yourself (DIY) ethos, and the use of technology for the purposes of fortifying masculinity. Keywords do-it-yourself, electric guitar, masculinity, popular music, technology, sound California Noise: Tinkering with Hardcore and Heavy Metal in Southern California Steve Waksman Tinkering has long been a part of the history of the electric guitar. Indeed, much of the work of electric guitar design, from refinements in body shape to alterations in electronics, could be loosely classified as tinkering. -
1 What Is Photography?
Basic Photography To P. Still the first for the first . Basic Photography Seventh Edition Michael Langford FBIPP, HonFRPS Formerly Photography Course Director Royal College of Art, London Focal Press OXFORD AUCKLAND BOSTON JOHANNESBURG MELBOURNE NEW DELHI Focal Press An imprint of Butterworth-Heinemann Linacre House, Jordan Hill, Oxford OX2 8DP 225 Wildwood Avenue, Woburn, MA 01801-2041 A division of Reed Educational and Professional Publishing Ltd A member of the Reed Elsevier plc group First published 1965 Second edition 1971 Third edition 1973 Fourth edition 1977 Fifth edition 1986 Sixth edition 1997 Reprinted 1998 (twice), 1999 Seventh edition 2000 © Michael Langford 2000 All rights reserved. No part of this publication may be reproduced in any material form (including photocopying or storing in any medium by electronic means and whether or not transiently or incidentally to some other use of this publication) without the written permission of the copyright holder except in accordance with the provisions of the Copyright, Designs and Patents Act 1988 or under the terms of a licence issued by the Copyright Licensing Agency Ltd, 90 Tottenham Court Road, London, England W1P 0LP. Applications for the copyright holder’s written permission to reproduce any part of this publication should be addressed to the publishers British Library Cataloguing in Publication Data A catalogue record for this book is available from the British Library Library of Congress Cataloguing in Publication Data A catalogue record for this book is available from the -
Godin Guitars Are Assembled in Berlin New Hampshire from Parts Crafted in Québec Canada
Godin Guitars are assembled in Berlin New Hampshire from parts crafted in Québec Canada. Les Guitares Godin sont assemblées à la main aux États-Unis à partir de pièces fabriquées au Canada. Godin Guitars, 19420 Avenue Clark-Graham, Baie D’Urfé Québec Canada H9X 3R8 www.godinguitars.com Godin is a registered trademark of 117506 Canada inc. / Godin est une marque déposée de 117506 Canada inc. All specifications are subject to change without prior notice. / Toutes les caractéristiques sont sujettes à changements sans préavis. Printed in Canada on Recycled Paper / Imprimé au Canada sur papier recyclé Photography & Graphic Design / Photos et Conception Graphique : Katherine Calder-Becker For over thirty years, Robert Godin has been pushing the bound- aries of both acoustic and electric guitar design. Robert’s innova- tions in semi-acoustic guitars, multi-voice instruments, and synth access have made Godin an industry leader in all of these cate- gories. Godin’s ongoing commitment to value and innovation has never been more evident than in the current model lineup. All Godin guitars are assembled in Berlin, New Hampshire from parts crafted in Godin's original facility in La Patrie, Quebec Canada. Robert Godin godin glossary Ergocut - This describes a special technique that is applied to all Godin necks. The sides SA (synth access via the 13-pin connection) - Godin has led the industry in the design and of the neck—at the fingerboard—are beveled inward providing an extra comfortable "worn development of guitars with built-in synth access for over a decade. The eleven synth- in" feel. ready models in the line are equipped with a 13-pin output jack that connects the guitar directly into various 13-pin devices. -
The Great Indoors
The Great Indoors CATALOGUE SPRING 2018 Contents 2 New Titles Ceramic Design What I’ve Learned The Other Office 3 8 Previously Announced Grand Stand 6 Suppose Design Office Identity Architects Built Unbuilt 12 Recent Titles Jo Nagasaka / Schemata Architects Studio O+A Happening 2 Night Fever 5 16 Portfolio Highlights Where They Create Japan Sound Materials Powershop 5 Knowledge Matters Spaces for Innovation CMF Design Goods 2 Holistic Retail Design 20 Backlist 29 Index 30 Distributors 32 Contacts & Credits 2 NEW TITLES 3 CERAMIC DESIGN New Wave Clay The unprecedented surge in popularity of 44 Ceramic Design 45 Bruce Rowe By day, Bruce Rowe runs the Melbourne ceramics brand Anchor Ceramics, which produces handmade lighting, tiles and planters on an almost industrial scale, selling to homes, architects and the hospitality trade across Australia. Then Rowe swaps making ceramics for the built environment in order to make the built environment ceramics in the last five years has helped forge out of clay. The structures he creates are composed of architectural elements BRUCE assembled in strange compositions: constructions made of stairs, passageways, bridges and arches. They are never literal images of actual architecture, but creative interpretations with a De Chirico-like surreal quality. “The works do not directly reference any specific place, building or structure. Whilst educated in the structural and spatial language of architecture, my a new type of potter: the ceramic designer. ROWE practice combines this knowledge with an inquiry into the symbolic function and aesthetic activity of visual art,” he says. Rowe began making small, singular pieces and progressed into series of structures that make up a whole. -
Electric Guitar and Bass Design: the Guitar Or Bass of Your Dreams, from the First Draft to the Complete Plan by Leonardo Lospennato Book
Electric Guitar and Bass Design: The guitar or bass of your dreams, from the first draft to the complete plan by Leonardo Lospennato book Ebook Electric Guitar and Bass Design: The guitar or bass of your dreams, from the first draft to the complete plan currently available for review only, if you need complete ebook Electric Guitar and Bass Design: The guitar or bass of your dreams, from the first draft to the complete plan please fill out registration form to access in our databases Download here >> Paperback:::: 218 pages+++Publisher:::: Leonardo Lospennato (October 11, 2010)+++Language:::: English+++ISBN-10:::: 3000296425+++ISBN-13:::: 978-3000296420+++Product Dimensions::::8 x 0.5 x 10 inches++++++ ISBN10 3000296425 ISBN13 978-3000296 Download here >> Description: Are you ready to design the ax of your dreams? Unlike other DIY guitar making sources (which deal with woodworking, mostly) this book focuses on how to achieve astonishing looks, excellent playability and a killer sound. Written collaboratively with top luthiers from the U.S. and Europe, this inspiring book covers in depth each aspect of electric guitar design, plus topics not found on other sources, like: - How to design a classic, how to design a radical - Thousand-year wisdom applied on Stratocasters and Les Pauls - Secrets of sustain - How to create a well balancing guitar - Ergonomics - How to choose, match and place pickups - Control design - The 4 laws of wood selection ... And much more. These 220 pages will have you making sketches from the first read! Not so much a how-to do guide as it is a why to do book.You wont find any step-by-step instructions or how-to chapters. -
Talk History of Photography 2.Pptx
History of Photographic Technologies By Dan Hyde Camera equipment for wet-plate collodion photography (1851) – George Eastman Museum March 22, 2018 History of Photograph Tech.-Dan Hyde 1 Photography is one of the visual arts with roots in drawing and painting. March 22, 2018 History of Photograph Tech.-Dan Hyde 2 Etching Technology of 1700s Etching by Albrecht Dürer The Cannon, 8” x 13”, 1518 • Etching is a method of making prints. Well established by 1500s. • Start with a copper plate. Place on acid-resistant ground (used bitumen, a natural asphalt). • With a sharp tool (pointed etching needle), the design is scratched into the ground exposing the copper. • Use a strong acid to etch the plate which eats away the areas of the plated unprotected by the ground, forming a pattern of recessed lines. • The remaining ground is cleaned off. The plate is inKed all over, and then the inK wiped off the surface, leaving only the inK in the etched lines. • These lines hold the inK, and when the plate is applied to moist paper, the design transfers to the paper making a finished print. March 22, 2018 History of Photograph Tech.-Dan Hyde 3 Portable Camera Obscura • Aristotle in 4th century BC Knew the principles of Camera Obscura - light through a pinhole in a darKen room forms an inverted image of outside scene on a surface. • By 16th century lenses had replaced the pinhole creating a brighter and more focused image. A mirror was added to reverse the image. • In 17th century the Camera Obscura was combined with a tent and made portable.