Efficient Professional and Antibody Platforms 1 Antibody Basic information: Catalog No.: UPA62509 Source: Rabbit Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: Protein A affinity purified Useful Information: WB:1:1000-1:2000 Applications: ICC:1:50-1:200 IHC:1:50-1:200 Reactivity: , Mouse, , Dog, Pig Specificity: This antibody recognizes Calponin 1 protein. Immunogen: recombinant protein Calponin regulates cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an - and Tropomyo- sin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth through its interaction with F-Actin and inhi- bition of the Actin-activated MgATPase activity of phosphorylated . Description: Both properties are lost following (primarily at Serine 175) by protein C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Cal- ponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. Uniprot: P51911(Human) Q08091(Mouse) Q08290(Rat) Q08092(Pig) BiowMW: 33 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of Calponin on Hela cell ly- sates using anti-Calponin antibody at 1/1,000 di- lution.

ICC staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraf- fin-embedded human breast tissue using an- ti-Calponin antibody. Counter stained with he- matoxylin.

Immunohistochemical analysis of paraf- fin-embedded mouse bladder tissue using an- ti-Calponin antibody. Counter stained with he- matoxylin.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]