Wnt10a Antibody Cat. No.: 4639

Wnt10a Antibody

Immunofluorescence of Wnt10a in Human Skeletal Muscle Immunohistochemistry of Wnt10a in human skeletal tissue with Wnt10a antibody at 20 μg/mL. muscle tissue with Wnt10a antibody at 10 μg/mL.

Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

September 28, 2021 1 https://www.prosci-inc.com/wnt10a-antibody-4639.html Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. IMMUNOGEN:

The immunogen is located within amino acids 300 - 350 of Wnt10a.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

SPECIFICITY: This Wnt10a antibody is predicted to not cross-react with Wnt10b.

POSITIVE CONTROL: 1) Cat. No. 1283 - RAW 264.7 Cell Lysate

Predicted: 46 kDa PREDICTED MOLECULAR WEIGHT: Observed: 46 kDa

Properties

PURIFICATION: Wnt10a Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

Wnt10a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: WNT10A

ALTERNATE NAMES: Wnt10a Antibody: OODD, SSPS, STHAG4, Wnt-10a

ACCESSION NO.: Q9GZT5

PROTEIN GI NO.: 14424011

GENE ID: 80326

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

September 28, 2021 2 https://www.prosci-inc.com/wnt10a-antibody-4639.html Background and References

Wnt10a Antibody: Wnt10a is a member of the Wnt family, a family that encodes secreted signaling that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors BACKGROUND: and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.

1) Kelly GM, Lai CJ, and Moon RT. Expression of wnt10a in the central nervous system of REFERENCES: developing zebrafish. Dev. Biol. 1993; 158:113-21.

2) Nusse R and Varmus RE. Wnt . Cell 1992; 69:1073-87.

3) Kirikoshi H, Sekihara H, and Katoh M. WNT10A and WNT6, clustered in human 2q35 region head-to-tail manner, are strongly coexpressed in SW480 cells. Biochem. Biophys. Res. Commun. 2001; 283:798-805.

4) Kirikoshi H, Inoue S, Sekihara H, et al. Expression of WNT10A in human cancer. Int. J. Oncol. 2001; 19:997-1001.

ANTIBODIES FOR RESEARCH USE ONLY.

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