Phytotaxa 306 (3): 234–236 ISSN 1179-3155 (print edition) http://www.mapress.com/j/pt/ PHYTOTAXA Copyright © 2017 Magnolia Press Correspondence ISSN 1179-3163 (online edition) https://doi.org/10.11646/phytotaxa.306.3.6

A new species of Paris sect. Axiparis () from , China

YUNHENG JI1*, CHENGJIN YANG2 & YULING HUANG1,3 1Key Laboratory for Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kun- ming, China; e-mail: [email protected] 2Chinese Medicinal Resources Co. LTD, Yunnan Baiyao Group, Kunming, China. 3University of Chinese Academy of Sciences, Beijing, China. *author for correspondence

Paris Linnaeus (1573: 367) is a temperate of about 27 species of perennial herbs distributed in Eurasia (Li 1998, Ji et al. 2007). Most species are restricted to East Asia, chiefly to China (19 species), with the Yunnnan- Plateau in southwest China as the centre of species diversity (Li 1998). Paris is well known in China for its medicinal value. Those species with a thick rhizome have been used as medicinal herbs for more than 2,000 years in China owing to its analgesic, haemostatic, anti-tumor, and anti-inflammatory activities (Long et al. 2003). More than 40 commercial drugs and health products have been developed in China with Paris used as raw material (Li et al. 2015). Paris sect. Axiparis Li (1984: 359) is unique in the genus in its thick rhizome, ovary 4- to 7-loculed, axile placentation, and indehiscent-baccate fruit. Phylogenetic analyses based on nuclear ITS and chloroplast trnL-F, psbA-trnH resolved the section as a monophyletic group with strong statistical support (Ji et al. 2006). During a field survey in Tengchong County, Yunnan Province, southwest China in 2015, we discovered an unusual population with distinctive morphological features. After careful examination of specimens from the CDBI, E, IBSC, KUN, K, LE, PE, SM, SYS and SZ herbaria, and comparison of descriptions and keys of Hara (1969), Takhtajan (1983), Li (1998), Mitchell (1987, 1988), Liang & Soukup (2000) and Ji et al. (2007), we were able to hypothesize that these represent a species new to science, for which we here provide the formal description and illustration.

Description of the new species Paris tengchongensis Y.H. Ji, C.J.Yang & Y.L.Huang sp. nov. (Figs. 1, 2) Paris tengchongensis can be distinguished from other species in Paris sect. Axiparis by sepals with purple markings, reddish purple , an ovoid ovary, and a sub-globose berry. Type:—CHINA. Yunnan: Tengchong County, Houqiao Township, 3120 m, 25º34’02”N, 98 º16’23”, 14 April 2015, Y.H. Ji 1341 (holotype KUN!, isotype KUN!, PE!).

Perennial herb, rhizome cylindrical, oblique or horizontal, 1–4 cm in diameter, 3–10 cm long, bearing a bud at the top and fleshy roots ca. 4–20 cm long. Stem erect, cylindric, 40–60 cm tall. Leaves 4–7 in a whorl at the top of stem, green; petiole green, ca. 1.5–4.5 cm long; blade oblong to elliptic, 7–12×2–5 cm, base subcordate, apex acute; lateral veins one pair, basely developed, net veins obvious, 6–8 pairs. Peduncle yellowish green, 5–10 cm. Flower solitary, developing from top of stem. Sepals 4–7, ovate-lanceolate, 1.5–3cm×5–10 mm, with purple markings at base or along midvein. Petals 4–7, narrowly linear, reddish purple, 3–6 cm×2–3 mm. Stamens 2-whorled, filaments 4–8 mm, anther yellow, dehiscing by lateral slit, 5–10 mm long. Ovary 4- to 7-loculed, red, ovoid, placentation axile; stigmas 4–7, distinct, 5–15 mm long. Berry indehiscent, red, subglobose. Seeds ovoid, slightly enveloped by spongy aril. Additional specimens examined (paratypes):—CHINA. Yunnan. Tengchong County: Houqiao Township, 3200 m, 25º33’57’’N, 98º16’58”, 16 August 16 2015, Y.H. Ji & C.J. Yang 021 (KUN!); Gudong Township, 2810 m, 25º33’17”N, 98 º16’08”, 18 August 2015, Y.H. Ji & C.J. Yang 029 (KUN!); Jietou Township, 2950 m, 25º32’06”N, 98 º15’44”, April 2016, Z.M. Wang s.n. (KUN!). Distribution and ecology:—The new species is endemic to China, where it is only found on the west slope of Gaoligongshan Mountains in Tengchong County, Yunnan Province. The plant grows in bamboo thickets and conifer forests at 2750–3200 m a.s.l. It flowers in April, and its fruit reaches maturity in August.

234 Accepted by Lorenzo Peruzzi: 8 Mar. 2017; published: 12 May 2017 FIGURE 1. Paris tengchongensis. A. ; B. sepal; C. ; D. stamen; E. pistil; F. transverse section of ovary showing axile placentation; G. berry; H. transverse section of berry; I. seed enveloped by imperfect aril. Illustration drawn from the holotype by Dongyan Wang.

FIGURE 2. Paris tengchongensis. A. habitat; B. habit; C. rhizome and roots; D. leaves (abaxial and adaxial surface); E. sepals (abaxial and adaxial surface); F. pistil; G. transverse section of ovary showing axile placentation; H. berry; I. transverse section of berry; J. petals; K. stamens; L. seeds enveloped by imperfect aril.

Etymology:—The specific epithet “tengchongensis” is named after the type locality, Tengchong County, Yunnan Province, China. Taxonomic relationships:—On the basis of its thick rhizome, ovary 4- to 7-loculed, axile placentation, fruit an indehiscent berry, and seeds enveloped by an imperfect aril, Paris tengchongensis is clearly a member of P. section Axiparis H.Li, a section so far represented by five species: P. axialis Li (1984: 273), P. dulongensis H.Li & S.Kurita in Li (1992: 14), P. forrestii (Takhtajan 1983: 268) Li (1984: 359), P. rugosa H.Li & S.Kurita in Li (1992: 13), and P. vaniotii Léveillé (1906: 355). Compared with other species in the section, P. tengchongensis shows closest affinity with P. forrestii. These two species share oblong to elliptic leaf blades that are subcordate at the base and acute at the apex, the petals longer than the sepals, and a red berry. However, P. tengchongensis is easily distinguished from P. forrestii in its sepals with purple markings (vs.

A new species of Paris sect. Axiparis Phytotaxa 306 (3) © 2017 Magnolia Press • 235 markings lacking), reddish purple petals (vs. greenish yellow petals), and sub-globose berry (vs. angular berry). In addition, P. forrestii has been collected in evergreen broad-leaved forests at 1900–2500 m a.s.l. (Li 1998), whereas P. tengchongensis occurs at of 2750–3200 m a.s.l. or higher, where bamboo thickets and conifer forests predominate. Conservation status:—Paris tengchongensis is only known from three localities, and there are fewer than 1,000 mature individuals at each locality. The plant is commonly harvested as medicinal herbs by local people, which could result in continuing decline of population size and occupancy. According to the IUCN (2012) red list categories and criteria, P. tengchongensis should be assessed as Endangered, EN B2ab(iii, v). We assume that human activities in the region could lead them to rapid extinction.

Acknowledgements The authors thank Dongyan Wang for drawing the illustration of the new species, and Zhangming Wang, Chaoze Li, Yanchang Zhang, and Shengyan Huang for help in field investigation. This work was supported by National Natural Science Foundation of China grant 31070297 to Yunheng Ji.

References Hara, H. (1969) Variations in Smith with reference to other Asiatic species. Journal of the Faculty of Science, University of Tokyo, Sect 3: 141–180. IUCN (2012) IUCN Red List Categories and Criteria: Version 3.1. Second edition. Gland, Switzerland and Cambridge, UK: IUCN, 32 pp. Léveillé, H. (1906) Liliacées, Amaryllidacées, Iridacées et Hemodoracées de Chine. Memorie della Pontificia Accademia Romana dei Nuovi Lincei 24: 333–379. Ji, Y., Fritsch, P.W., Li, H., Xiao, T. & Zhou, Z. (2006) Phylogeny and classification of Paris (Melanthiaceae) inferred from DNA sequence data. Annals of Botany 98: 245–256. https://doi.org/10.1093/aob/mcl095 Ji, Y., Zhou, Z. & Li, H. (2007) Four new synonyms in the genus Paris (). Acta Phytotaxonomica Sinica 45: 388–390. https://doi.org/10.1360/aps06163 Li, H. (1984) Two new species of the genus Paris. Acta Botanica Yunnanica 6: 273–276. Li, H. (1984) The phylogeny of the genus Paris L. Acta Botanica Yunnanica 6: 351–362. Li, H. (1998) Classification of the genus Paris L. In: Li, H. (Ed.) The Genus Paris (Trilliaceae). Science Press, Beijing, pp. 8–65. Li, H. & Noltie, H.J. (1997) Miscellaneous notes on the genus Paris. Edinburgh Journal of Botany 54: 351–353. https://doi.org/10.1017/S0960428600004170 Li, H. (1992) New taxa of the genus Paris. Acta Botanica Yunnanica 5 (Suppl.): 13–18. Li, H., Su, B., Yang, Y.M. & Zhang, Z.Y. (2015) An assessment on the rare medicinal Paris plants in China with exploring the future development of its plantation. Journal of West China Forestry Science 44: 1–6. Liang, S.Y. & Soukup V.G. (2001) Paris L. In: Wu, Z.Y. & Raven, P.H. (Eds.) Flora of China 24. Science Press, Beijing, and Missouri Botanical Garden Press, St. Louis, pp. 88–95. Linnaeus, C. (1753) Species Plantarum. Impensis Laurentii Salvii, Holmiae, 1200 pp. Mitchell, B. (1987) Paris–part I. Plantsman 9: 81–89. Mitchell, B. (1988) Paris–part II. Daiswa. Plantsman 10: 169–190. Takhtajan, A. (1983) A revision of Daiswa (Trilliaceae). Brittonia 35: 255–270. https://doi.org/10.2307/2806025

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