NCAPH Antibody Cat. No.: 30-178

NCAPH Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human NCAPH.

TESTED APPLICATIONS: ELISA, WB

NCAPH antibody can be used for detection of NCAPH by ELISA at 1:12500. NCAPH APPLICATIONS: antibody can be used for detection of NCAPH by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate

PREDICTED MOLECULAR 82 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

October 2, 2021 1 https://www.prosci-inc.com/ncaph-antibody-30-178.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: NCAPH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: NCAPH

ALTERNATE NAMES: NCAPH, BRRN1, CAP-H, HCAP-H

ACCESSION NO.: NP_056156

PROTEIN GI NO.: 25121987

GENE ID: 23397

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

NCAPH is a member of the barr family and a regulatory subunit of the complex. This complex is required for the conversion of interphase into condensed . The is associated with mitotic chromosomes, except during the early phase of condensation. During interphase, the protein has a distinct punctate nucleolar localization.This encodes a member of the barr gene BACKGROUND: family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization.

REFERENCES: 1) Beausoleil, S.A., (2006) Nat. Biotechnol. 24 (10), 1285-1292.

ANTIBODIES FOR RESEARCH USE ONLY.

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