Additional Table 1 Information of quantitative polymerase chain reaction primers

NM_204117.1 Gallus gallus neuregulin 1 (NRG1), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity ACGATACC Forward TATGCAGG Plus 20 1350 1369 59.61 55 4 2 primer TGCC TGGTACAG Reverse TTCCTCAG Minus 21 1466 1446 59.38 52.38 5 0 primer CTTCC Product 117 length

NM_001302142.1 Gallus gallus S100 calcium binding B (S100B), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity GTCCCCTC Forward GGTTCTAC Plus 20 15 34 62.55 65 2 2 primer GCAC TCCTTCCC Reverse GGAGTACT Minus 20 507 488 60.61 60 6 2 primer GGTG Product 493 length

NM_001031598.1 Gallus gallus , beta 3 class III (TUBB3), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity TCCTCTCA Forward CAAGTACG Plus 20 163 182 59.4 55 6 2 primer TGCC CCCCGCTC Reverse TGACCGAA Minus 19 289 271 60.08 57.89 5 2 primer AAT Product 127 length

NM_001305054.1 Gallus gallus growth associated protein 43 (GAP43), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity CGGCCGAG Forward AACAAAGA Plus 20 313 332 60.04 55 6 1 primer GAGT GGTTTCTT Reverse CTACAGCA Minus 23 746 724 58.17 43.48 6 2 primer TCTGTCT Product 434 length

NM_001101730.1 Gallus gallus , medium polypeptide (NEFM), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity CACCACCT Forward ATCAGGAC Plus 21 1053 1073 59.24 52.38 4 3 primer ACGAT GGGTCCAG Reverse TGATGCTT Minus 20 1251 1232 60.39 60 4 2 primer CCAG Product 199 length

XM_417679.6 PREDICTED: Gallus gallus neurofilament, light polypeptide (NEFL), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity AAGACGCT Forward GGAGATCG Plus 20 978 997 60.18 55 4 2 primer AAGC CACCTTCC Reverse AGCAGTTT Minus 21 1221 1201 60.48 52.38 3 1 primer CCTGT Product 244 length

XM_004942359.3 PREDICTED: Gallus gallus marker of proliferation Ki-67 (MKI67), transcript variant X1, mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity GCAGGTTC Forward TTCATGTTC Plus 21 641 661 59.12 47.62 4 3 primer AGCA TCTTTGCC Reverse CTCTGCAT Minus 23 742 720 59.8 43.48 4 3 primer TATCTGA Product 102 length

NM_204170.2 Gallus gallus proliferating cell nuclear antigen (PCNA), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity AATGCGGA Forward TACGTTGG Plus 20 284 303 59.82 50 4 0 primer CTCT TGTACTCC Reverse ACAACGAG Minus 20 719 700 57.72 50 4 2 primer AGGA Product 436 length

NM_001039258.2 Gallus gallus cadherin 1 (CDH1), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity AGCCAAGG Forward GCCTGGAT Plus 20 1435 1454 59.81 55 5 1 primer TATG CCATTGTC Reverse CACAGCCA Minus 20 1830 1811 60.03 55 4 3 primer GGAT Product 396 length

NM_001031229.1 Gallus gallus , alpha 2, , aorta (ACTA2), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity TCTGGGCG Forward TACTACAG Plus 20 439 458 59.74 55 5 2 primer GGAT AAAGGAGT Reverse AGCCACGT Minus 20 606 587 60.67 55 4 2 primer TCGC Product 168 length

NM_001198712.1 Gallus gallus fibronectin 1 (FN1), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity CGGCAATG Forward GCAACAGA Plus 20 7535 7554 59.97 50 4 0 primer CAAA GTCGGGAA Reverse CGTTGGAC Minus 20 7930 7911 60.25 55 6 3 primer TCAA Product 396 length

NM_205131.1 Gallus gallus zinc finger E-box binding homeobox 1 (ZEB1), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity ATCCCACC Forward AAGTGCCA Plus 20 2388 2407 60.32 55 3 0 primer ATCC TCCTGCCT Reverse TTCGTCCT Minus 20 2626 2607 60.83 55 4 2 primer GACT Product 239 length

NM_204486.2 Gallus gallus transcription factor 3 (TCF3), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity ATAAAGCG Forward CGGAACAG Plus 20 2307 2326 60.04 50 4 1 primer GTCA CTCGTCGC Reverse CGCCGTTT Minus 21 2452 2432 62.17 52.38 6 3 primer AAAAT Product 146 length

NM_001199487.1 Gallus gallus Fas cell surface death receptor (FAS), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity AAGCACTC Forward GGTTTGGA Plus 20 388 407 59.82 50 3 0 primer GGTT TCACAATG Reverse TCAGGGAC Minus 20 705 686 59.97 55 5 3 primer GTGG Product 318 length

NM_001167701.1 Gallus gallus caspase 2 (CASP2), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity GGACACTC Forward ATGTGGCT Plus 20 1140 1159 59.96 55 4 3 primer GACA TCCCGACA Reverse GAGCGTAC Minus 20 1268 1249 60.04 55 5 3 primer TACA Product 129 length

NM_001302097.1 Gallus gallus phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity GTCCTTCG Forward CTACACAG Plus 20 453 472 60.39 60 5 3 primer TGGG ATGCAAAG Reverse GCAACAGG Minus 20 555 536 60.47 50 4 0 primer GGAA Product 103 length

XM_421073.6 PREDICTED: Gallus gallus Fas associated via death domain (FADD), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity TTCAGTCG Forward CTTCGAGA Plus 20 528 547 59.76 55 4 2 primer GTGG CATACCCG Reverse TGGTCTGA Minus 20 706 687 59.1 55 6 0 primer GTGT Product 179 length

NM_204305.1 Gallus gallus glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA Self Self 3' Sequence Template Length Start Stop Tm GC% complement complement (5'->3') strand arity arity AGTCAACG Forward GATTTGGC Plus 20 72 91 59.68 50 4 2 primer CGTA ACAGTGCC Reverse CTTGAAGT Minus 20 230 211 60.18 55 5 3 primer GTCC Product 159 length