Product Description EN Bactoreal® Kit Salmonella Enterica

Product Description

BactoReal® Kit Salmonella enterica

Order no. Reactions Pathogen Internal positive control

DHUB02553 50 FAM channel Cy5 channel

For Research Use Only. Not for use in diagnostic procedures.

Kit contents:  Detection assay for Salmonella enterica  Detection assay + target for internal DNA positive control (control of PCR amplification and DNA extraction)  DNA reaction mix  Positive Control for Salmonella enterica  Nuclease-free water

Background: Salmonella is a genus of Gram-negative bacteria of the Enterobacteriaceae family. This genus consists of only two species, Salmonella enterica and Salmonella bongori. Salmonella bongori mostly infects cold blooded animals. Salmonella enterica has six subspecies and more than 2500 serovars. Subspecies are S. enterica subsp. arizonae, S. enterica subsp. diarizonae, S. enterica subsp. enterica, S. enterica subsp. houtenae, S. enterica subsp. indica and S. enterica subsp. salamae. Salmonella infections in warm-blooded animals are generally caused by strains from S. enterica subspecies enterica. Typhoidal serovars include Salmonella Typhi and Salmonella Paratyphi, which only infect humans. Nontyphoidal serovars are more common, and usually cause self-limiting gastrointestinal disease. They can infect a range of animals, and are zoonotic. Although many other Salmonella serovars may cause enteric disease, the more common ones in cattle are Salmonella Typhimurium, Salmonella Dublin and Salmonella Newport. In sheep and goat the most common are Salmonella Typhimurium, Salmonella Dublin, Salmonella Abortusovis, Salmonella Anatum and Salmonella Montevideo. In pigs the most common are Salmonella Typhimurium and Salmonella Choleraesuis, and in poultry Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum and Salmonella Pullorum.

Description: BactoReal® Kit Salmonella enterica is a real-time PCR test detecting DNA of the invasion protein A (invA) gene of Salmonella enterica. The invA gene is essential for full virulence in Salmonella and is specific for members of the genus Salmonella. The kit allows the rapid and sensitive detection of S. enterica from DNA samples purified from swabs, feces, etc (e.g. with QIAamp DNA Mini Kit). Salmonella enterica DNA is detected in FAM channel. The internal DNA positive control (DNA IPC) is detected in Cy5 channel and is used as DNA extraction as well as PCR inhibition control. The DNA IPC target is extracted with the sample.

PCR-platforms: This kit has been validated with Applied Biosystems® 7500 Fast Real-time PCR System (Thermo Fisher Scientific). It is compatible with real-time PCR instruments detecting and differentiating fluorescence in FAM and Cy5 channel.

Sensitivity and specificity: BactoReal® Kit Salmonella enterica detects at least 7 target copies/PCR reaction. The limit of detection (LoD95 = smallest number of copies of target DNA which can be detected in 95% of cases) is 19 target copies/reaction. The kit is specific for Salmonella enterica. Salmonella bongori is detected with less sensitivity.

ingenetix GmbH BactoReal® Kit Salmonella enterica (DHUB) Arsenalstraße 11, 1030 Vienna, Austria Product description v1.1en T +43 (0)1 36 198 0 198 F +43 (0)1 36 198 0 199 [email protected] www.ingenetix.com Page 1 of 2

Product Description

Logarithmic view Linear view

ABI Prism® 7500: FAM channel, 530 nm 1:10 serial dilution of a Salmonella enterica DNA target

ABI Prism® 7500: FAM channel, 530 nm Salmonella enterica positive control

ABI Prism® 7500: Cy5 channel, 667 nm Internal DNA positive control

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