L: LABORATORY BIOASSAYS Management Tests, Vol. 21 413

Cabbage maggot (CM); (L.) Richard Meadow (20L) Norwegian Crop Research Institute Plant Protection Centre Fellesbygget N-1432 As, Norway John D. Vandenberg USDA-ARS Plant Protection Research Unit U.S. Plant, & Nutrition Laboratory Tower Rd. Ithaca, NY 14583 Downloaded from https://academic.oup.com/amt/article/21/1/413/4641601 by guest on 28 September 2021 Anthony M. Shelton Department of Entomology Cornell University NYSAES 416 Barton Lab Geneva, NY 14456 D. Wesley Watson Department of Entomology Cornell University Ithaca, NY 14583

MICROBIAL CONTROL OF MAGGOT-PRELIMINARY SCREENINGS OF FUNGAL ISOLATES, 1995: Adult CM were exposed to dry conidia of isolates of Beauvaria bassiana (Bb), Metarhizium anisopilae (Ma) or Paeciomyces fumosoroseus (Pf). Two B. bassiana isolates were from Cornell University (P89, L90) isolated from Musca domestica (Diptera: ), one was from Mycoteh Corp., Butte, MT (Myc 726) originally isolated from southern corn rootworm, Diabrotica undecimpuntata (Coleoptera: Chrysomelidae), re-isolated from a grasshopper, Melanoplus sp. (Orthoptera:Acrididae), and then re-isolated from silverleaf whitefly, Bemesia argentiflora (Homoptera: Aleyrodidae). The other isolates were from the USDA-ARS Collection of Entomophagous Fungal Cultures. One B. bassiana isolate, 4012, was isolated from Delia radicum (Diptera: ). The P. fumosoroseus isolates were number 1626 from M. autumnalis (Diptera: Muscidae), 1644, 1645, 1867 and 1868 from M. domestica, 1646 from Calliphora spp. (Diptera: Calliphoridae), and 887 from unidentified Diptera. The M. anisopilae isolates were 2521 from Deois spp. (Homoptera: Cercopidae), 3540 from Gelleria mellonella (Lepidoptera: Pyralidae) and 4862 and 4865 from soil. from rearings at the New York State Agricultural Experiment Station, Geneva, NY, were exposed to the fungi by placing 5 flies in a centrifuge tube containing conidia scraped directly from culture plates. The flies were then released into small screened plastic cages, which were placed in chambers with constant temperature of 21° C and L:D 15: 9. There were 3 replicates. Mortality was assessed after 48, 120 and 160h. Dead flies were placed in petri dishes with moistened filter paper to assess infection. Isolate P89 (B. bassiana) caused mortality in the most flies after 48h. It was also the only isolate that gave 100% mortality and 100% infection at the end of the experiment. Isolates L90 and 2521 also caused fatal infection in more than 50% of the flies.

Mean no. of flies infected (hours post exposure) Fungus Isolate n 48h 120h 160h Control 5 0.0a 0.0a 0.0a Bb L90 5 0.0a 3.3a 3.3cd Bb P89 5 1.7a 5.0d 5.0d Bb M726 5 0.0a 1.7abc 1.7abc Pf 1626 5 0.0a 0.0a 0.0a Pf 1646 5 0.0a 0.0a 0.0a Pf 1645 5 0.0a 0.3ab 0.7ab Pf 1867 5 0.0a 0.0a 0.0a Pf 1868 5 0.3a 0.3ab 0.3ab Ma 3540 5 0.7a l.Oab 2.0bc Ma 4862 5 0.0a 0.0a 0.7ab Ma 2521 5 0.3a 1.3ab 2.7bc Bb 4012 5 0.3a 2.0bc 2.0bc Ma 4865 5 0.0a 0.0a 0.0a Pf 887 5 0.3a 0.3ab 0.3ab Pf 1644 5 0.7a 0.7ab 0.7ab Means within columns followed by the same letter are not significantly different (P = 0.05) by Tukey.