RBI Annual Report 2010

design skala design ISSN 1334-854X ISSN

RBI Annual Report 2010.

Ruđer Bošković Institute

Annual Report 2010

Zagreb, 2013. Editorial Board: Editor: Nela Pivac

Members: Tome Antičić David Smith Kata Majerski Mery Sopta Stjepko Fazinić Karolj Skala Bojan Macan

Technical Support: Photographer: Josip Uhrl Graphic Design and Technical Processing: Franjo Matković Printing: PRINTERA GRUPA d.o.o. Print run of 500 copies

Web edition: http://www.irb.hr/eng/About-the-RBI/Annual-Reports

Address: Ruđer Bošković Institute Bijenička cesta 54, P.O.Box 180 HR-10002 Zagreb, Croatia

Contact: URL: http://www.irb.hr e-mail: [email protected] tel: +385 1 45 61 111 fax: +385 1 46 80 084

Theoretical Physics Division...... 11

Division of Experimental Physics...... 17

Division of Materials Physics...... 25

Division of Laser and Atomic Research and Development...... 31

Division of Electronics...... 37

Division of Physical Chemistry...... 41

Division of Organic Chemistry and Biochemistry...... 47

Division of Materials Chemistry...... 55

Division of Molecular Biology...... 61

Division of Molecular Medicine...... 69

Division of Marine and Environmental Research...... 75

Centre for Marine Research...... 83

Centre for Informatics and Computing...... 87

Centre for Nuclear Magnetic Resonance...... 91

Library...... 95 Dear reader,

Welcome to the 2010 Annual Report of the Ruđer Bošković Institute. The aim of this report is to provide a succinct overview of the most important activities and top achievements made at the Ruđer Bošković Institute during 2010. As such, the report covers exemplary performance in high-quality fundamental research, published in top journals or scientific books, and shows the strong involvement of scientists of the Ruđer Bošković Institute in higher education. The report also highlights selected awards, recognitions, patents, domestic and international projects and collaborations, important invited lectures, and international conferences organized by the Institute during the year. On behalf of the Editorial Board, I would like to take this opportunity to sincerely thank the RBI staff for their cooperation during the preparation of this report. We would also like to thank you, the reader, for your interest and take pleasure in inviting you to share with us your comments and suggestions regarding future editions of this report.

Editor Nela Pivac Introduction

OVERVIEW sion, NATO, Cogito, COST, UNESCO and other international scientific foundations. The year of 2010 saw the Ruđer Bošković Today, the RBI has over 550 scientists and Institute (RBI) celebrate its 60th Anniversary. researchers in more than 80 laboratories pur- From its foundation in 1950, on the initiative suing research in theoretical and experimental of Ivan Supek and colleagues who chose to physics, material physics and chemistry, elec- name the Institute after the renowned Croa- tronics, physical chemistry, organic chemistry tian physicist Ruđer Bošković, the Institute and biochemistry, molecular biology and medi- has come a long way. Today, the RBI is re- cine, marine science and the environment, in- garded as Croatia’s leading scientific institute formational and computer sciences, laser and in the natural and biomedical sciences as well nuclear research and development. as marine and environmental research, owing In addition to basic research, the RBI is to its size, scientific productivity, international strongly involved with the development of in- reputation in research, and the quality of its novative research, participation in higher ed- scientific personnel and research facilities. ucation and increasing public awareness of The Institute is the leading and most interna- the importance of knowledge and science in tionally competitive Croatian institute by virtue modern society. These tasks are conducted of its participation in international research in cooperation with universities, scientific in- projects, such as the IPA, IAEA and FP5-7 stitutes and other similar institutions in Croa- programs funded by the European Commis- tia and all over the world. RBI Annual Report 2010.

Figure 1. The organizational structure of the RBI

1 ORGANIZATION OF THE Governors, the Director General and the INSTITUTE Scientific Council. Important input is derived from the Heads of the Divisions and Centres Organizationally, the RBI consists of (via their Divisional Councils), the Assistant twelve divisions, three centres, a library, the Directors, as well as the Heads of the Ad- office of the Director General, as well as sec- ministration, the Maintenance and Technical tions for maintenance and technical services Services and the Library (Figure 2). The In- and administration (Figure 1). ternational advisory board provides vital ex- The administrative structure of the In- ternal advice and guidance. stitute sees central roles for the Board of

Figure 2. The administrative structure of the RBI RBI Annual Report 2010. RBI

2 Director General: Danica Ramljak Walter Giger, Swiss Federal Institute for Head of the Scientific Council: Neven Bilić Aquatic Sciences and Technology, Chairman of the Board of Governors: Dübendorf/Zürich, Switzerland Slavko Krajcar Thomas C. Malone, OceanUS Office for Integrated and Sustained Ocean International Scientific Board: Observations, Arlington, VA, USA Jean-Marie Lehn, Laboratoire de Chimie Werner E.G. Müller, Johannes Guten- Supramoléculaire, ISIS/ULP, France berg Universität, Mainz, Germany Harold Kroto, University of Sussex, UK Nadia Pinardi, University of Bologna, Egon Matijević, Clarkson University, NY, Ravenna, Italy USA Helmut Schwarz, Technische Universität Berlin, Germany ACTIVITIES Fritz Vögtle, Universität Bonn, Germany Robert Blinc, Jozef Stefan Institute, Ljublja- Fundamental research na, Slovenia Jonathan R. Ellis, CERN, Switzerland The total number of research articles pub- Anthony R. Peaker, University of Manches- lished by RBI scientists in 2010 was 613. ter, UK Among these, 477 were published in journals Bogdan Povh, MPI für Kernphysik, Heidel- cited by Current Contents, the majority of berg, Germany which were published in high ranking interna- Roberto D. Peccei, UCLA, Los Angeles, CA, tional Journals. USA The details of the many important discov- Fernando Azorin, Institute of Molecular Biol- eries made by RBI scientists in 2010 are to ogy of Barcelona, Spain be found in the subsequent sections of this Bernd Kaina, Institut für Toxikologie, Mainz, report. Nevertheless, we present here a small Germany section of highlights in order to convey a gen- Werner E. G. Müller, Johannes Gutenberg eral impression of the kind research carried Universität, Mainz, Germany out at the Institute. Miroslav Radman, Université René Des- Ideas that development of embryos mir- cartes-Paris V, France rors evolutionary history are well known even Jürgen Soll, Ludwig-Maximilians-Universität outside biological circles. However, due to the München, Germany lack of tools that would allow explicit quanti- Vito Turk, Jozef Stefan Institute, Ljubljana, tative testing, the scientific status of this elu- Slovenia sive concept has been unclear. Evolutionary Joseph Schlessinger, Yale University School RBI Annual Report 2010. geneticist, Tomislav Domazet-Lošo, from the of Medicine, CT, USA Division of Molecular Biology, together with Hans Joachim Seitz, Universität Hamburg, Diethard Tautz, from the Max Planck Institute Germany for Evolutionary Biology, devised a new ap- Davor Solter, MPI für Immunbiologie, proach that measures phylogenetic age of Freiburg, Germany the expressed genes across ontogeny and Peter J. Stambrook, University of Cincinnati showed that phylogeny-ontogeny correlation Medical Center, OH, USA is real. In a broader perspective, this study Rudolf Zechner, Institute for Molecular Bio- shows that the correlation between phylog- sciences, Graz, Austria eny and ontogeny is not a myth, and that Farooq Azam, University of California, San the once disputed ideas of von Baer, Darwin Diego CA, USA

3 and Haeckel have to be revisited. The results prestigious journal PLoS Genetics. The study were published in the 9th December issue of describes how natural selection acts to im- Nature as a cover story. prove speed and accuracy of protein transla- Nela Pivac, Matea Nikolac, Gordana Nedić tion, as evidenced in biased codon usage in and Dorotea Mueck-Šeler from the Laboratory prokaryotic genomes. The study represents a of Molecular Neuropsychiatry in the Division significant step forward in terms of its breadth, of Molecular Medicine, together with associ- computational methodology and this work ates from the Faculty of Pharmacy and Bio- points to a universal regularity - the contents chemistry at the University of Zagreb discov- of 'silent' sites of a group of genes in all bacte- ered a major advance in the diagnosis of the ria and archaea are not determined randomly. attention-deficit hyperactive disorder (ADHD). Rather, they are shaped by translational se- Their research article entitled Human plasma lection, which is a new and significant finding glycome in attention-deficit hyperactivity disor- from many perspectives. For instance, gene der and autism spectrum disorders was pub- expression levels can be predicted from co- lished in the prestigious journal Molecular and don usage and the expression level correlated Cellular Proteomics. The article details for the to environmental conditions, enabling further first time changes in specific glycan structures in silico research of mechanisms of microbial found in the plasma of 99 children suffering adaptation. from ADHD. These results indicate the possi- Irena Dokli, and Zdenko Hameršak, from bility of clinical applications and the elabora- the Laboratory for Stereoselective Catalysis tion of new potential biomarkers for diagnos- and Biocatalysis in the Division of Organic ing ADHD through a simple blood test. Chemistry and Biochemistry, in collaboration RBI Scientists Anamaria Brozović, Andreja with Ivana Matanović from the Theoretical Ambriović-Ristov, and Maja Osmak, from the Chemistry Group, in the Division of Physical Laboratory for Genotoxic Agents in the De- Chemistry, have published an article entitled partment of Molecular Biology have published Sulfur Ylide Promoted Synthesis of N-Pro- a review article entitled The relationship be- tected Aziridines: A Combined Experimental tween cisplatin-induced reactive oxygen spe- and Computational Approach in Chemistry - A cies, glutathione, and BCL-2 and resistance European Journal. The article describes the to cisplatin in the journal Critical Reviews in asymmetric synthesis of N-protected aziridi- Toxicology, which is one of the most respected nes in high enantiomeric excess, using a chi- journals in the field of toxicology. This review ral benzyl sulfonium salt. The diastereoselec- article describes the role and relationship of tivities of the reactions are influenced by the the anti-apoptotic protein BCL-2 and gluta- N-protecting group, the imine substituent, and thione in cisplatin-induced oxidative stress as the sulfide structure. In the case of tert-butyl well as the role these molecules play in cispla- substituted aziridines, an unusual cis selectiv- tin resistant tumour cells. Despite the general- ity was observed and subsequently explained ly accepted fact that DNA damage is a key for using computational models. the harmful effects of cytostatics, this review Analytical Chemistry, the world's leading article presents the results of recent studies journal in the field of the same name, published that show that cisplatin can induce production a paper entitled Blind separation of analytes of lethal reactive oxidative radicals (ROS) that in nuclear magnetic resonance spectroscopy are not primarily related to DNA damage. and mass spectrometry: sparseness-based Scientists from the Division of Electron- robust multicomponent analysis by Ivica Ko- ics, Fran Supek, Nives Skunca, Jelena Repar priva (Division of Laser and Atomic Research and Tomislav Šmuc, published an extensive and Development) and Ivanka Jerić (Division RBI Annual Report 2010. RBI computational study entitled Translational of Organic Chemistry and Biochemistry). The selection is ubiquitous in prokaryotes in the paper describes a method for blind extraction

4 of analytes from mixtures. The method is ex- petitive projects are provided by the Croatian emplified on the examples in NMR spectros- Science Foundation and the Unity Through copy and mass spectrometry. The original Knowledge Fund (UKF), from which RBI has contribution of the paper is theoretical and 13 active projects, respectively. The Institute is experimental demonstration of the extraction involved with almost 100 international projects of analytes the number of which exceeds the (including 7 FP7, 1 FP6, 6 IAEA, 3 NATO, 1 number of mixtures. It has been considered COST, 1 UNESCO, 1 SCOPES, and 58 bilat- within the chemometrics community so far that eral projects), as well as 61 applied and tech- this problem is too ill-posed and, thus, insolv- nological contracts (including 6 HRZZ, 1 Na- able. Potential application of obtained result is tional Park Plitvice, 3 iProject, 2 VIP, 1 HIT and in biomarker detection, natural product analy- 1 BICRO). The total financial value of these sis and metabolomics. The paper is a result projects can be seen in Table 1, as well as the of two years of collaborative efforts that have funding trends since 2006. already yielded three scientific papers and two Table 1 also shows that the largest non- international patent applications. competitive source of revenue continues to be derived from the MInistry of Science, Education and Sports, which directly con- Projects and other revenue tributed almost € 23 M in 2010. A significant part of this revenue is dedicated to staff sala- The RBI has 132 projects in basic research, ries (ca. 75%), while the remained is relat- which are funded by the Ministry of Science ed to various running costs (Table 2). This Education and Sport. Additional domestic com- so-called block grant was reduced in 2010.

Revenue 2006 % 2007 % 2008 % 2009 % 2010 %

Funding from the Ministry of Science, 20.842.974 88,05% 23.770.518 83,61% 25.755.013 86,37% 24.030.178 83,82% 23.372.730 81,61% Education and Sports (MSES)

National Projects funding (Croatian Science Foundation, 172.490 0,73% 1.017.194 3,58% 487.601 1,64% 345.916 1,21% 1.365.294 4,77% Croatian Institute of Technology, etc.)

International projects funding (FP, NATO, 749.913 3,17% 1.377.929 4,85% 910.305 3,05% 1.575.532 5,50% 1.780.549 6,22% IAEA, etc.)

Commercial contracts 1.782.322 7,53% 1.999.839 7,03% 2.449.662 8,22% 2.416.483 8,43% 1.873.087 6,54%

Donations and other 123.887 0,52% 264.046 0,93% 216.622 0,73% 300.392 1,05% 246.147 0,86% funding

Total 23.671.586 100,00% 28.429.526 100% 29.819.203 100% 28.668.501 100% 28.637.807 100% Table 1. RBI revenues from 2006-2010. RBI Annual Report 2010.

FUNDING FROM THE STATE 2006 % 2007 % 2008 % 2009 % 2010 % BUDGET

Salaries 14.194.651 68,15% 15.752.782 66,30% 17.082.656 66,63% 17.825.802 74,22% 18.243.201 76,23%

Operating costs 2.897.658 13,91% 3.052.403 12,85% 2.727.386 10,59% 1.908.463 7,95% 2.087.153 8,72%

Research projects - national 2.960.532 14,21% 3.711.388 15,62% 3.591.772 13,95% 2.659.660 11,07% 2.251.603 9,41%

Research projects - international 18.604 0,09% 25.290 0,11% 222.488 0,86% 124.391 0,52% 393.296 1,64%

International co-operation 197.193 0,95% 183.613 0,77% 160.847 0,62% 159.213 0,66% 155.848 0,65%

Construction and other investment 560.892 2,69% 1.034.174 4,35% 676.742 2,63% 915.769 3,81% 259.794 1,09%

Loan from the World Bank 1.281.309 4,98% 425.211 1,77% 540.973 2,26%

Total 20.829.530 100,00% 23.759.650 100,00% 25.743.200 100,00% 24.018.509 100,00% 23.931.868 100,00% Table 2. A breakdown of RBI revenues from the state budget from 2006-2010.

5 Thus, despite the increase in international project funding observed during 2010, the total RBI revenue was reduced by almost € 2 M in comparison to 2008.

Organization of international conferences

As in previous years, the RBI continued to support the organization of numerous international and domestic conferences. Many of them were organized as a part of a series of events celebrating the 60th Anniversary of the Institute. For example, a symposium on Ivan Supek - Scientist and Humanist was Figure 3. A brochure from the RBI’s “Open Days”. held in April at the RBI. Five plenary lectures ing the 60th Anniversary of the Institute. The were presented on the life and work of Ivan aim of this event was to inform the public Supek, Croatian physicist, philosopher, writ- with RBI research and to show how funds er, peace advocate, humanist and founder invested in science are used for the develop- of the RBI. Plenary lecturers included promi- ment of society, higher education and creat- nent scientists and academicians, Ksenofont ing better living conditions for all citizens. Ilakovac, Ivo Šlaus, Vladimir Paar, Kruno As part of the celebrations marking the Pisk, and Vladimir Knapp. 60th anniversary of RBI, the Center for Infor- In April the Department of Experimental matics and Computer Science and the De- Physics organized its firstFestival of Science partment of Electronics at RBI held a meeting by young researchers. The festival showed on May, commemorating 60 Years of Elec- the recent research of doctoral students in tronics and Computer Science Research at the department. RBI. Among the numerous renowned scien- For the fourth year in a row, the RBI orga- tists from Croatia and abroad attending the nized its Open Days. In 2010, this event took event, Professor Veljko Radeka, recipient of place from May 6th to 8th and attracted over the Harold Wheeler Award and director of the 4500 visitors, not only from Zagreb, but also Instrumentation Division at the Brookhaven from all over Croatia and neighbouring Bos- National Lab (USA) was also a participant. nia and Herzegovina. Visitors were treated The meeting focused on the development of to an exhibition with 16 focal points which electronics and computer science at RBI over were designed to bring the world of science the last 60 years and many of the scientists in closer to the general public. attendance began their careers in the area of The Scientists of the Institute held a se- electronics and computer science at RBI. ries of popular lectures on current topics in In cooperation with the government of the various fields of science. The visitors had the Republic of Croatia and the RBI, the IAEA opportunity to visit the exhibition "Croatian Regional Workshop on Successfully Apply- inventors through history" which showed the ing for EU-funded Projects was held in May rich tradition of invention and patent protec- at the RBI. The workshop brought together tion in Croatia. The exhibition was organized sixty participants from twenty countries in by the State Intellectual Property Office in Europe and Central Asia. cooperation with RBI's spin-off company RBI Annual Report 2010. RBI At a gala event held in the Vatroslav Lisin- Ruđer Innovations Ltd. The RBI Open House ski Concert Hall the 60th anniversary of RBI was organized as part of the activities mark-

6 was celebrated in June. Croatian president attended by 124 scientists from both Croatia Dr. Ivo Josipovic, as well as Premier Jadran- and abroad. This conference brings together ka Kosur were patrons of the event. President chemists, biochemists, molecular biologists Josipovic in his address pointed out the work and bioinformaticists from Central European of RBI has tremendous importance for Croa- countries representing a platform for the dis- tia. He stated that there should be better co- semination of research results arising from operation between the educational process, collaborations during the previous two years science and industry. Josipovic also stated and provides an opportunity to initiate plans that science should not be subject to austerity for new collaborations. The most common measures as this undermines our future. themes at the conference were the chemistry of proteins and peptides, structural biology, bioprocesses and drug design. The Laboratory for Ion Beam Interactions hosted the first workshop organized within the FP7 SPIRIT project valued at 7 million euros. The workshop entitled New detector technologies for advanced materials research using ion beam analysis was held in October at Plitvice National Park. The aim of the planned workshops was to inform a broad range of scientists on the possibilities for use of accelerator methods in scientific Figure 4. The celebration of the 60th anniversary of RBI at the research in the areas of physics, chemistry, Vatroslav Lisinski Concert Hall. biology and medicine and thereby increase both the number of users as well as the The third in a series of summer schools availability of European accelerator infra- in applied molecular microbiology entitled structure. The total number of participants at Microbial Metabolites: Signals to Drugs was the Plitvice workshop was 60 scientists from held in August in Dubrovnik. The Summer 15 European countries, including a number School is co-organized by the John Innes of speakers from partner institutions within Centre (UK) and the RBI. The summer the SPIRIT project. school is intended for scientists at the gradu- More details about the conferences, con- ate level and post-doctoral fellows as well as gresses and meetings organized by the RBI those who plan a career in industry. can be found in the subsequent pages of this The RBI in cooperation with leading insti- report. tutions in the field of nanotechnology: Finn- ish Centre for Nanotechnology, University of Awards and Recognition RBI Annual Report 2010. Jyvaskyla and Joanneum Research in Graz organized first session of Adriatic School on As in previous years, the achievements of Nanoscience - ASON-1, in September in Du- RBI scientists were also recognized through brovnik. The school brought together lead- numerous awards. For example, Morana Ja- ing Croatian and European speakers in the ganjac received prestigious State Award for fields of nanoscience as well as participants Science in the graduate student category, from several countries. for the significant scientific contribution in the In September in Primošten, the RBI orga- study of the role of neutrophils in the growth nized the international scientific conference and regression of tumours. This work has led 5th Central European Conference - Chem- to the application for an international patent istry Towards Biology. The conference was pertaining to her work.

7 Young scientist Snježana Jurić received Education prestigious Željko Trgovčević Award, for research excellence in the field of molecular Although the RBI is currently not able to biology and genetics. At the 180th conference conduct its own, independent postgraduate of the Hungarian Academy of Sciences, its study program, it has developed collabo- President Professor József Palinkas awarded rations with several universities in Croatia Karolj Skala, the RBI's head of the Centre for through a number of doctoral and specialist Informatics and Computing, with a medal for study programs. In addition, through basic scientific research and international coop- agreements with various universities in Croa- eration. In addition, the Centre for Informatics tia, RBI scientists actively participate in pro- and Computing received the VIDI e-Novation grams of higher education in Croatia. Owing Award Golden Tesla's Egg. to the large number of potential mentors and At a meeting of the European Association qualified researchers, as well as the relatively for Cancer Research (EACR), Sonja Levanat modern research equipment, the RBI remains from the Department of Molecular Medicine an attractive place for the best students from was elected to honorary membership in the Croatian universities to pursue doctorates, EACR. As well Sonja Levanat received ca. € which continue to provide an influx of quali- 70,000 of Terry Fox Run Donation. This dona- fied doctoral candidates. Table 3 and Figures tion would aid Sonja Levanat's work which is 5 and 6 provide an overview of the level of aimed at research and treatment of hereditary courses and their distribution across different forms of breast and ovarian cancer. locations and Divisions, respectively. Robert Vianello was awarded the Promis- ing Scientist Award at the 15th international Type of course Number of conference on Quantum Systems in Chem- courses istry and Physics. The award was given by Undergraduate courses 76 Graduate courses 33 the Centre for Applied Quantum Mechanics Ph.D. courses 181 in Paris for his significant contributions to the Additional specialist courses 9 computational design of extremely strong su- Total 299 peracids and superbases and insights into their properties. Table 3. Overview of the courses held by RBI in 2010. The Director's Prize for Most Successful RBI Scientists have been awarded to almost Intellectual Property 50 RBI scientists, for publication in high impact journals, successful grant applications (grants Apart from fundamental research and greater than ca. € 70,000), projects of special education, the activities of the RBI also re- importance to RBI, successful international sult in various forms of intellectual creations. patent applications as well as for scientific One important aspect of the RBI’s mission work honoured with state awards for science. is the protection of intellectual property and The International Conference on Informat- its commercialisation. In this context, various ics and Communication Technologies, Elec- specific activities have been initiated and tronics and Microelectronics - MIPRO 2010 realized over the last few years. As a sub- awarded the RBI with the MIPRO Charter for sidiary, the Ruđer Bošković Institute estab- exceptional service in the development of ICT, lished Rudjer Innovations Ltd., a company electronics and microelectronics. specializing in the commercialization of inno- List of all the awards and recognition given vations and technology transfer. The intellec- to the RBI scientists can be found in the in- RBI Annual Report 2010. RBI tual property portfolio of Rudjer Innovations dividual Divisional reports in the subsequent includes innovations protected by patents or sections of this report. patent applications in various fields of sci-

8 Figure 5. The distribution of the RBI courses by the location of the Figure 6. The distribution of the RBI courses by RBI Division relevant hosting institution ence that have commercial potential. In 2010 ed amino acids for use as antitumor drugs, RBI was granted by three patents. No. P20050242), to several investigators The State Intellectual Property Office of the including RBI scientists Štefica Horvat, Kata Republic of Croatia granted a patent for the in- Majerski, Andreja Jakas and Jelena Veljković. novation NN'N''-Tris-(3-dimethylaminopropyl)- The United States Patent and Trademark guanidine, No. P20040341, the procedure of Office granted a patent for the discovery of preparation from carbodiimide and application a Mixed bacterial culture for atrazine degra- in reactions of the transesterification of oil, to dation, to a team including Dubravka Hršak, the inventors Mirjana Maksić and Zoran Gla- from the Department for Marine and Envi- sovac, researchers from the Laboratory for ronmental Research at the RBI. The patent Physical-Organic Chemistry in the Division of describes a new mixed bacterial culture with Organic Chemistry and Biochemistry. specific properties that quickly and complete- Similarly, the domestic Intellectual Property ly degrades atrazine and other s-triazite pesti- Office granted a patent entitledSynthetic pep- cides over a wide concentration range and at tides containing unnatural adamantane relat- different temperatures. RBI Annual Report 2010.

9 STAFF ARTICLES* THESES

Division Articles in other Articles Articles in Theses (written Theses (menthored by Research journals Total in CC conference Books Handbooks Patents by RBI staff) RBI staff) staff and articles journals proceedings chapters in books Ph.D. B.Sc. Ph.D. M.Sc. B.Sc. Theoretical 24 28 1 29 2 3 Physics Department Department of 48 121 5 4 130 24 2 1 2 Experimental Physics Department 28 35 1 2 38 1 4 of Materials Physics Department 7 7 2 9 of Laser and Atomic Research and Development Department of 11 5 3 3 11 2 1 Electronics Department 41 55 14 2 71 1 6 1 of Physicsl Chemistry Department 65 50 7 57 2 7 2 4 of Organic Chemistry and Biochemistry Department 40 22 7 8 37 3 of Materials Chemistry Department 67 30 10 40 6 4 1 12 of Molecular Biology Department 71 76 16 1 93 10 6 2 16 of Molecular Medicine Department 79 52 15 11 78 1 1 7 2 2 1 of Marine and Environmental Research Centre for 37 12 5 8 25 1 6 1 Marine Research Centre for 6 1 3 6 10 Informatics and Computing Centre for 11 14 1 15 1 1 1 Nuclear Magnetic Resonance Library 1 1 TOTAL RBI 535 477 87 49 613 3 24 3 46 7 13 14 40

Table 4. Review of publications for the year 2010 (*Original scientific papers and review papers in journals/conference proceedings and

RBI Annual Report 2010. RBI chapters in books.)

10 cosmological constant isformulatedasa cosmological gravity story –amodified control cosmologicalconstantunder A large TOP ACHIEVEMENTS OVERVIEW OFTHEDIVISION their B.Sc.,M.Sc.andPh.D.thesis. of Split and a number of students completed the UniversityofZagreband sion continuedtobeinvolvedinlecturingat Divi- the of members The systems. complex condensed matterphysics,nanophysicsand is alsoasubstantialresearchcomponentin general andmathematicalphysics. There modynamics, gravityandcosmology, and chro- quantum perturbative physics, quark tion ofhigh-energyphysics,includingheavy- Physics Division covers theoretical investiga- consists ofthefollowinglaboratories: The (ZTF) Theoretical PhysicsDivision Head: BrankoGuberina ORGANIZATION DIVISIONAL The mechanism for the relaxation of the of relaxation the for mechanism The Research performedinthe Theoretical Theoretical PhysicsDivision a a a

Physics Group, Stjepan Meljanac Physics Group,Stjepan Theoretical andMathematical Group, NevenBilić Particle PhysicsandCosmology Brako Radovan Solid StatePhysicsGroup, eidc crdne Hmloin Remark- Hamiltonian. Schrödinger periodic related a of eigenstates Bloch-Floquet of set orthogonal to thecomplete correspond which operators annihilation and creation bosonic nian fluctuation was diagonalized in terms of BPS solutions. Hamilto The corresponding these around fluctuations quadratic of ysis waves. Weanal a complete havepresented model supportsperiodicdensity Calogero Model Density Waves intheCalogero PeriodicFluctuations around BPS- rived (Meljanacetal.,2010). the sector Snyder geometryis underlying de- of thecoalgebraic The genericconstruction commutative spacetimewasconstructed. commutative Snyder Space-Time onNon- Scalar FieldTheory damental theories(Štefančićetal.,2010). that could be expected from high-energy fun- constant ofbothsigns ily largecosmological tically reducetheeffects ofalmostarbitrar- dras- gravity of modifications that introduced the was shown It theory. gravity modified The collective field formulation of the formulation field collective The non- Snyder the on theory field scalar A http://www.irb.hr/en/ztf - -

11 2010. Report Annual RBI ZTF 12 RBI Annual Report 2010. ZTF in aon-topsite,upperpanel,andhollow site,lowerpanel. and thenon-localvdW-DF functional,forCOadsorbedon Pt(111) Functional Theory calculationswiththestandardPBEfunctional Fig. 1.Differences incorrelationenergydensitiesbetween Density order to resolve the discrepancies between to resolvethediscrepancies order faces of late 4d and5dtransitionmetals.In sites ofdifferenton (111) coordination sur- into molecules CO of adsorption the of tions the incorrect predictionsin ab-initio calcula puzzle adsorption andtheCO non-local correlation with Density functionaltheory 2010). the vacuum energy density (Duplančić et al., of isnotfullyrepresentative density iltonian Ham- the of value expectation vacuum the arebroadand distributions tained probability in Minkowskispacewaspresented. The ob- fields scalar massless complex and real for density ofthevacuumenergy ity distribution densities One vacuum,manypossibleenergy sity wave’s amplitude (Bardeketal.,2010). of the parameterwhichdeterminesden- ably, the fluctuation spectrum is independent The well-known CO adsorption puzzle is puzzle adsorption CO well-known The of the probabil calculation An analytical - - energy ofthesystem(Lazić etal.,2010). a newimproved valueforthetotal obtaining DFTusing any standard calculated codethus density to thecharge the functional applying used in a post-DFT approach,by calculation computer codewhichis massively parallel successful. Wea JuNoLo, developed have LDA andGGA functionalshavenotbeen plays animportantrole,wherethestandard thevanderWaalsrials inwhich interaction to softmatterandothermate- calculations of applying theDensityFunctional Theory vdW-DFtion functional opens theprospect energy functional correlation vanderWaals-densityevaluation of post-processing Massively parallel the contacts(Crljenetal.,2010). tries at the expense of geome- weaker coupling with nonequilibrium at orbitals frontier better band alignment between relevant increases in the conductance arise from geometry. It is shown that all the predicted gap equilibrium the for conductance lowest played withthecontactseparation the unusualincreaseofconductanceisdis- However, observations. experimental with the contractionofgapisshowninline increase inthelowbiasconductancewith The method. function Green’s equilibrium using density-functionaltheoryandnon- try onthetransporthasbeeninvestigated story molecular wire a geometry: nonequilibrium gap conductancein of Increase (Lazić etal.,2010). improves thevalueofadsorptionenergy and discrepancies solves thesitepreference sidered casesthisreducesorcompletely correlation. In all con- includes nonlocal der Waals-densityvan new which functional calculations, wehaveappliedtherelatively theory functional density and experimental The recently developed nonlocal correla nonlocal The recentlydeveloped gap geome- a contact of influence The - AWARDS Education (GRB) data(Meljanacetal.,2010). burst gamma-ray the of fit multiparameter a through relations canbeobtained persion dis- the in coefficients the how shown also have and corrections quadratic on bounds that some of provide certain the realizations is proposed,withinwhichwehaveshown mutative κ-Minkowski spacetime realizations time delaywithrespecttodifferent noncom and relations dispersion modified describing eter) arecompared. A general framework param- (deformation scale gravity quantum accuracy inthe lay uptothesecond-order time de- vs. energy)andthecorresponding vs.momenta andmomenta lations (energy spacetime -Minkowski kappa scale from onthequantumgravity Constraints Gravity (July2007–December2011). network QuantumGeometryand the SteeringCommitteefor ESF research lence andLarisaJonkeservedasamemberof Excel- Scientific for Award Director’s RBI the received trampetić Josip and Štefančić voje Jurman, Stjepan Meljanac, Hrvoje Nikolić, Hr- or M.Sc.(3)theses. their B.Sc.(2) Split. Fivestudentscompleted of Zagreband courses attheUniversities andgraduate as lecturersinundergraduate Twore- dispersion versionsofdeformed Velimir Bardek, Radovan Brako, Danijel Danijel Brako, Radovan Bardek, Velimir In 2010,membersoftheDivisionserved - 7. 6. 5. 4. 3. 2. 1. Science,Education andSports of bytheMinistry supported Projects projects 1. Selected invitedlectures 4. 3. 2. 1. project international Research, developmental and es andsymmetries,StjepanMeljanac Quantum field theory, noncommutative spac- Jonke Matrix models, duality and field theory, Larisa physics, Josip Trampetić energy spaces inhigh Noncommutative cle physicsandcosmology, BrankoGuberina Fundamental interactionsinelementaryparti- Waals-Casimir forces, Marin-Slobodan Tomaš der van the fluctuations: field Electromagnetic tures, ŽeljkoCrljen Electronic propertiesof hybrid nanostruc Radovan Brako calculations, cal approachesandnumerical Surfaces andnanostructures: Theoreti- and beyond, invited talk given at the workshop and beyond,invitedtalkgiven attheworkshop L0 at DVCS from GPDs K, Passek-Kumerički HEPTOOLS, no.MRTN-CT-2006-035505) to theFW6EURTN(collaboration Network Trampetić Josip Colliders, at Discoveries ics Tools forPhys- andPrecisionCalculations (Croatian-Serbian BilateralProject) Štefančić Hrvoje universe, the of expansion Modified gravity theories and the accelerated ship HubertCurien) partner- - “COGITO” program the in Project (Croatian-French Melić Blaženka B-decays, D- Final state and interaction innon-leptonic gen, Germany) vestigators from RBI and Universityof Sie- in- von HumboldtFoundation,withprincipal Alexander the by promoted Project search Re- (International Melić Blaženka hadrons, QCD sum rules for exclusive decays of heavy -

13 2010. Report Annual RBI ZTF Diffractive and electromagnetic processes at SELECTED ORGANIZED LHC, Trento, Italy, January 4-8, 2010 CONFERENCES 2. Bilić N, Cosmological k-essence condensa- ZTF tion. Beyond 2010, Cape Town, South Africa, 1. Beyond 2010, Cape Town, February 1-6, February 1-6, 2010 2010 (Nevenko Bilić, member of the Organ- 3. Andraši A, Energy Divergences in Coulomb izing Commitee) Gauge QCD, Triangle Workshop on Non- 2. Croatian Black Hole School, Trpanj, Pelješac, Perturbative Methods in Qunatum Field The- Croatia, June 21-25, 2010 (Zoran Škoda, ory, Heviz, Hungary, March 10-12, 2010 member of the Organizing Commitee) 4. Škoda Z, Twisted nonabelian differential co- 3. 5th Austrian-Croatian-Hungarian Meeting, homology, Conference «Geometry at large Summer Workshop for Theoretical Physics II: Hodge and homotopy theories», University – „Gauge Fields, Quark Matter, and LHC“, of Vienna, Vienna, Austria, May 3-7, 2010 Rab, Croatia, August 30 – September 4, 5. Nikolić H, Making nonlocal reality compat- 2010 (Anđelka Andraši, member of the Or- ible with relativity, Quantum 2010: Advances ganizing Commitee) in Foundations of Quantum Mechanics and 4. 3rd International Conference on Nuclear and Quantum Information with Atoms and Pho- Particle Physics with CEBAF at Jefferson tons. Torino, Italy, May 24-28, 2010 Lab, NAPP 2010, Dubrovnik, October 3-8, 6. Melić B, Charmless two-body B-meson de- 2010 (Blaženka Melić, member of the Organ- cays in QCD, 3rd Workshop on Theory, Phe- izing Commitee) nomenology and Experiments in Heavy Fla- 5. Meeting of Croatian Particle and Astroparti- vour Physics (Capri2010), Anacapri, Island cle Physicists, Ruđer Bošković Institute, Za- Capri, Italy, July 5-7, 2010 greb, Croatia, October 28-29, 2010 (Goran 7. Bilić N, Cosmological condensation of the Duplančić, member of the Organizing Com- tachyon fluid, Efeitos Quanticos em Gravi- mitee) tacao e Cosmologia, Espirito Santo, Brasil, August 15-20, 2010 8. Nikolić H, Making Bohmian mechanics Selected publications compatible with relativity and quantum field theory, 21st century directions in de Broglie- 1. Bauer F, Sola J, Štefančić H. Dynamically Bohm theory and beyond, Vallico Sotto, Italy, avoiding fine-tuning the cosmological con- August 29 – September 3, 2010 stant: the «Relaxed Universe». J Cosmol 9. Jonke L, Gauge theory on kappa-Minkowski re- Astropart P 12 (2010), 029-1. visited: the twist approach, Satellite Workshop 2. Battisti M V, Meljanac S. Scalar Field Theory on Noncommutative Field Theory and Gravity, on Non-commutative Snyder Space-Time. Corfu, Greece, September 8 - 12, 2010 Phys Rev D 82 (2010), 024028. 10. Bilić N, Cosmological condensation of the 3. Bardek V, Feinberg J, Meljanac S. Fluctua- tachyon k-essence, 6th Mathematical Phys- tions around Periodic BPS-Density Waves in ics Meeting: Summer School and Conference the Calogero Model. J High Energy Phys 8 on Modern Mathematical Physics, Belgrade, (2010), 018. Serbia, September 14-23, 2010 4. Duplančić G, Glavan D, Štefančić H. Prob- 11. Škoda Z, Symmetrization map, realizations ability distribution of the vacuum energy den- and Lie algebroids, «Higher Structures in sity. Phys Rev D 82 (2010), 125008-1. Mathematics and Physics», Erwin Schroed- 5. Bilić A, Crljen Ž, Gumhalter B, Gale J D, inger Institute for Mathematical Physics, Vi- Rungger I, Sanvito S. Conductance of a phe- enna, Austria, October 14, 2010 nylene-vinylene molecular wire: Contact gap RBI Annual Report 2010. RBI and tilt angle dependence. Phys Rev B 81 (2010), 155101-1.

14 6. Lazić P, Alaei M, Atodiresei N, Caciuc V, Bra- nar cavity. Phys Rev A 81 (2010), 044104-1. ko R, Blügel S. DFT with nonlocal correlation: 9. Borowiec A, Gupta K S, Meljanac S, Pachoł A key to the solution of the CO adsorption A. Constraints on the quantum gravity scale puzzle. Phys Rev B 81 (2010), 045401-1. from kappa - Minkowski spacetime. Euro- ZTF 7. Lazić P, Atodiresei N, Alaei M, Caciuc V, physics letters 92 (2010) 20006. Blügel S, Brako R. JuNoLo - Jülich Non Local 10. Zlatić V, Gabrielli A, Caldarelli G. Topological- code for parallel post-processing evaluation ly biased random walk with application and of vdW-DF correlation energy. Comput Phys community finding in networks. Phys Rev E Commun 181 (2010), 371. 82 (2010), 066109-1. 8. Tomaš M S. Recursion relations for general- 11. Palle D. On the anomalous large-scale flows ized Fresnel coefficients: Casimir force in a pla- in the Universe. Eur Phys J C 69 (2010), 581. RBI Annual Report 2010.

15 RBI Annual Report 2010. RBI

16 O as well as re- as well sor testinganddevelopment, and detectorandsen- ing nanotechnology includ research physics, interdisciplinary beam, nuclear,and astroparticle particle ion experimental involve Physics perimental verview oftheDivision laboratories: (DEP) consistsofthefollowing The DivisionofExperimentalPhysics Head: Tome Antičić ORGANIZATION DIVISIONAL The core activities of the Division of Ex- of Division the of activities core The Division ofExperimentalPhysics a a a a a a a a a Alfred Švarc Group forhadronicspectroscopy, methods, JasminaObhođaš Laboratory fornuclearanalytical physics, KrešoKadija Laboratory forhighenergy low-level activities,BogomilObelić Laboratory formeasurementof interactions, MilkoJakšić Laboratory forionbeam weak interactions,MilicaKrčmar Laboratory for electromagnetic and physics, RaulHorvat Laboratory forastroparticle Zoran Basrak Laboratory fornuclearphysics, Ivan Supek Laboratory forhadronphysics, - • • • • next fiveyearsarebriefly: of DEPobjectives strategic The main forthe its experimentalcapabilities. used, among others, to significantly enhance nancing from non-MZOS sources, which was fi- its of fraction large a received reputation, The Division, due to its excellent international physics research. in bothbasicandapplied a strongperformance Croatia, andmaintain in and abroad complexes experimental and experiments numerous in involved are They about 65staff,more than30PhDs. including applications. lated radiation has The division experiments. the anticoincidencegermanium system ton accelerator(neutron generator),or experimental such astheCockcroft Walcapabilities, - local on focus Additional local RBI Tandem acceleratorfacility. the at experiments for capacities and capabilities experimental Increased tions. institu- European with prominent levels at numerous Strengthened partnerships role intheseexperiments. DEP to amuchmoreprominent site, leading the at work experimental volving physics collaborations,inparticularly- experimental international contribution intoplarge enlarged significantly A http://www.irb.hr/en/zef

17 2010. Report Annual RBI ZEF 18 RBI Annual Report 2010. ZEF Compact Muon Sollenoid (CMS) detector has Compact Muon Sollenoid (CMS) detector has to Europe.Between April and November, the back the energy frontier in Particle Physics at highenergyandhas thereforebrought data first its taken has (LHC) Collider Hadron Collider (CERN) data Hadron atFirst theLarge T • • • • • • muons observedwiththeCMSexperiment. Fig. 1.FirstobservationofaheavyZbosondecayintopair op achievements After many years of preparation, the Large After manyyearsofpreparation,theLarge nal funding. exter- getting in success larger even An dents, aswellforcurrenttopstaff. Increased attractionfortopCroatianstu- research areas. interdisciplinary in techniques based tion of radia applications and development Traditionallyregarding strong activities local culturalinstitutionsandauthorities. and Rijeka, Croatian industry, as well of Zagreb,Split such astheUniversities with partners, Improved cooperation materials andtheirmodification. of Improved characterization advanced objects lyingontheseefloor. sensors for detection and identification of water vehicle,whichintegratesseveral of anautomatedunder Development - - axion emission signal from signal emission axion tolook forahigh-energy y-ray calorimeter the CASTby using photons to two and cleon to anu- that couple particles of pseudoscalar the relationbetweencouplingconstants axions hadronic Search for high-energy event isassessed(Agafonovaetal.,2010). the of significance the and detail in explained ground calculationsandtheircross-checkare cal selectioncriteriaaredescribed. The back- charged-current event satisfying the kinemati- topology and kinematics of a first candidate ντ interactions intheemulsion/leadtarget. The ticipated indatatakingandanalysisofneutrino event intheOPERAexperiment ντcandidate afirst of Observation of MaterialsPhysics. with the silicon ionsin Division collaboration SrTiO on thesurfaceof of chainsnanohillocks angleresultedincreation grazing incidence Ion beamsfor nanostructuring the productionofheavyWandZbosons. focusing on electroweak processes involving measurements, published first the and data played an active role in the analysis of the first also group RBI The environment. acquisition its contribution to the improvement of the data pated in the data collection and has continued The RBIgroupinCMShasactivelypartici- accumulated 36inversepicobarnsofdata. and D(p,γ) and (Andriamonje etal.,2010). f hn Ge/SiO thin of afterirradiation induced nocrystals hasbeen of Gena- ion beams.Furthermore,ordering using research modification materials in ity the versatility of the RBI accelerator facil- At CERN, the CAST-RBI group explored group CAST-RBI the CERN, At par- has group OPERA-RBI the LNGS, At Irradiation by swiftheavyionsunderthe Irradiation 3 (Karlusic et al.,2010),demonstrating (Karlusic 3 He (5.5 MeV) nuclear transitions nuclear MeV) (5.5 He 2 utlyr b oye and oxygen by multilayers 7 Li (0.478 MeV) (0.478 Li three-nucleon forces is not sufficient to de- to sufficient not is forces three-nucleon models. The current modelingof two and force sensitivity of the presentthree-nucleon 2 sion measurementofthebreak-upreaction nucleon forces Spin-isospin selectivityinthree- Horvat, 2010.) cosmological horizonanditsinterior. (R. tum-mechanical correlationsbetweenthe the entanglement entropy, measuring quan- capable of accounting for a strong jump in all holographicdarkenergymodelsisnot models energy dark Holographic to the (see inFig.2). These resultsarecompared which correspondtothe were observed for kinematical configurations predictions the dataandtheoretical between discrepancies Significant effect. forces on three-nucle including Faddeev calculations with state-of-the-art powers arecompared terium target. vector-analyzing The obtained beam of 190 MeV impinging on a liquid-deu angle forthereaction Fig. 2. The analyzingpowerasafunctionofthe centerofmass 190 MeV(squaredots). H(p,pp)n Nuclear force is studied Nuclear via apreci- force isstudied We showed that a framework underlying 2 H(p,d)n reactiontotest the isospin inducedbyapolarized-proton 2 H(p, 2 He)n inducedbypolarizedprotonsof 2 H(p, 2 He)n channel - - channel system(Tiator et al, 2010). multi- a of channels known experimentally for the structure relevant the pole determine to applied sequentially be can method this about allof the channels, mental knowledge experi full of absence the In data. channel single- from structure pole the extracting of We concluded that the RMisareliablemethod amplitudes. model meson-exchange of Dubna-Mainz-Taipeitigate singularities (see Cecietal,PRD77116007) toinves- method regularization the Zagreb applied identification confirmed methodfor resonance New Zagreb Phys. Lett.BandRev. Cjournals. lished thesemeasurementsintheprestigious nel. The CBCollaborationhasalreadypub- and resonance structure for eta meson chan- periments measuringdifferential crosssection ex- include work this of Highlights program. tal used this beam to continue with its experimen- μA. The Crystal Ball Collaboration successfully 20 of current beam a with GeV 1.6 achieved MAMI-C accelerator with BallCollaboration Crystal modefor the Full production isospin states(Mardanpouretal.,2010). data forboth scribe consistentlypolarization Fig. 3:NewMainzMicrotron(MAMI-C)racetrack. In collaboration with theMainzgroupwe In collaboration finally accelerator MAMI-C the 2010 In -

19 2010. Report Annual RBI ZEF Zagreb amplitude resonance on VME ADCs and MIDAS software. DAQ parameters updated records both coincident events and singles scaled down for appropriate factor. ZEF Zagreb realization of Carnagie-Melon- Berkeley amplitudes (as explained in Batinic Upgraded dual irradiation beam line et al, PR C51 2310) have been updated and suplemented with the pole positions (Batinic The unique possibility to simultaneous ir- et al, 2010). radiate materials with ion beams from both RBI accelerators, namely 1.0 MV Tandetron and 6.0 MV Tandem Van de Graaff, was New equipment made possible after installation of a new scattering chamber shown in Figure 5. This Setup for reaction products chamber has been equiped with 6 axis go- measured in coincidence niometer for sample positioning and on line monitoring of radiation damage induced by A modular array of 6 silicon strip detectors one ion beam while making analysis using each of 50x50 mm2 active area was set up, another ion beam (Fig. 5). tested and applied. Telescopes consist of 20 µm thick strip detector (SD) divided into 16 equal strips and 1000 µm or 500 µm thick position sensitive strip detector with perpendic- ularly mounted 16 strips (PSSD). Such a setup makes possible particle identification for all reaction products from proton to oxygen (see in Fig. 4) while its granularity and large- angle coverage allows identification and detection with high efficiency of the 8Be exit channel through the α-particle – α-particle coincidence. The energy resolution of the detected reaction products is in the range 40 and 100 keV. This detector array has 288 outputs which are processed by usual NIM electronics and data acquisition system build

Fig 5. New dual beam irradiation chamber. 6 axis goniometer enables performance of channeling RBS. Inset at botom right presents RBS/c imaging of silicon crystal planes.

Development of 14C AMS dating method

Graphite sample preparation (target for

RBI Annual Report 2010. RBI Fig. 4. The SD-PSSD telescopes mounted in the INFN-LNS Cata- 14 nia scattering chamber. Beam is coming from down. Inset depicts AMS) from micro samples for C dating has high particle-identification resolution. been further developed for different types of

20 material, including charcoal, bone, carbon- PROJECTS ate, DIC and DOC in water, etc. (Krajcar Bronić et al., 2010). Projects supported by the Ministry od Sciences, Education and Sports ZEF

1. Experimental research of the nucleus: nuclear structures and reactions, Suzana Szilner 2. Ion beam interactions and nanostructures, Milko Jakšić 3. Hadronic physics and QCD, Ivan Supek 4. Heavy-ion physics, Zoran Basrak 5. Massive neutrinos and astro-particles: from particle physics to cosmology, Ante Ljubičić 6. Experimental physics at LHC energies, Krešo Kadija 7. Experiments in quantum communication and quantum information, Mario Stipčević 8. Photon-atom interactions and correlations, Fig. 6 Vacuum line for preparation of graphite for 14C AMS Tihomir Surić measurement technique. 9. Natural isotopes in investigation of karst environment and dating, Bogomil Obelić Education 10. Development and application of nuclear analytical methods, Jasmina Obhođaš Members of the Division were involved 11. Development of the methods for illicit traffick- in lecturing at undergraduate and graduate ing control, Dario Matika and Davorin Sudac courses at the Faculty of Science at the Uni- 12. Hadronic physics between the experiments versity of Zagreb, University of Rijeka, and and QCD models, Alfred Švarc at joint studies organized by RBI in coopera- 13. Test of special relativity by the Ives-Stilwell tion with the Universities of Osijek and Du- type experiment, Saša Blagus brovnik. Four students completed their Ph. D. theses and one B. Sc. thesis under the Research, developmental and leadership of division staff. international projects

1. FP7 REGPOT project: “Particle Detectors”, AWARDS Tome Antičić project coordinator 2. Underwater Costal Sea Surveyor (UNCOSS),

Raul Horvat, Neven Soić, Tome Antičić Jasmina Obhođaš (EU FP7-SEC-2007-1, RBI Annual Report 2010. and Bogomil Obelić received the RBI Direc- Contract No. 218148. tor’s Award for scientific excellence in 2010. 3. Support of Public and Industrial Research Ines Krajcar Bronić served as a member of using Ion beam Technology (SPIRIT), Milko the ICRU Report Committee on “Key data Jakšić (EU FP7 project No. 227012) for measurement standards in the dosimetry 4. Network in solid waste and water treatment of ionizing radiation”, and Bogomil Obelić between Europe and Mediterranean countries served as a member of the Expert Advisory (SOWAEUMED), FP7 Project, Bogomil Obelić Group (EAG) “Regional aspects of the 7th 5. CLUustering phenomeNA in nuclear physics: Framework Programme” of EC Research Di- strengthening of the Zagreb - Catania – Bir- rectorate (2008-2012). mingham partnership (CLUNA), Neven Soić (FP7-REGPOT-2007-3 International Coop-

21 eration - Coordination and Support action, 17. CERN Axion Solar Telescope (CAST) experi- project number 203200) ment, Milica Krčmar (International collabora- 6. Hadron Physics 2, Roman Čaplar, Ivan Su- tion between RBI and CERN (Switzerland))

ZEF pek, Alfred Švarc, Ivan Supek activity lead- 18. ALICE collaboration, Tome Antičić (Inter- er in SPINMAP subprogram (EC FP 7 pro- national collaboration between RBI and gramme contract 227431. CERN(Switzerland)) 7. Research project Experimental nuclear 19. NA61 collaboration, Krešo Kadija (Interna- physics inputs for thermonuclear runaway, tional collaboration between RBI and CERN principal investigator Neven Soić, part of the (Switzerland)) collaborative project Physics of compact ob- 20. CMS Collaboration, Krešo Kadija, Vuko jects: explosive nucleosynthesis and evolu- Brigljević (International collaboration be- tion, EuroGENESIS programme of the Euro- tween RBI and CERN, Switzerland). pean Science Foundation 21. NA49 collaboration, Tatjana Šuša (Interna- 8. Using environmental isotopes for evaluation tional collaboration between RBI and CERN of streamwater/groundwater interactions in (Switzerland)) selected aquifers in the Danube basin (IAEA 22. Pierre Auger Observatory, Krešo Kadija, as- Reg TC project RER8016, Nada Horvatinčić) sociate membership through University of 9. Strengthening sustainability of nuclear re- Nova Gorica, Slovenia. search and development institutes in the modern science and technology environment, Stjepko Fazinić (National Coordinator, Selected invited lectures IAEA regional project RER/0/031) 10. Isotopic composition of precipitation in Croa- 1. Bogdanović Radović I, Coincidence elastic tia and Slovenia, bilateral project Croatia – scattering and TOF-ERDA – sensitve tech- Slovenia, Ines Krajcar Bronić (2009 – 2010) niques for light element analysis in thin films, 11. Measurement of 3H activity in natural waters 13th Joint Vacuum Conference, Štrbske Ple- with electrolytic enrichment, bilateral project so, Slovakia, June 20-24, 2010. Croatia – Slovenia, Jadranka Barešić (2010 2. Bogdanović Radović I, Light elements analy- – 2011) sis using elastic scattering coincidence tech- 12. Physics of nuclei and hadrons at high ener- nique at the Zagreb microprobe, 10th Euro- gies, Roman Čaplar (International collabora- pean Conference on Accelerators in Applied tion project RBI-KFKI Research Institute for Research and Technology, ECAART 2010, Particle and Nuclear Physics, Budapest via Athens, Greece, September 13-17, 2010 Croatian (HAZU) and Hungarian Academy of 3. Lakić B, Search for solar axions with the Science) CAST experiment. «ASPERA National Day 13. Monitoring of Marina Punat, Jasmina Croatia», Opatija, Croatia, May 27, 2010 Obhođaš 4. Lakić B, Search for solar axions with the 14. Development of frozen spin polarized target CAST experiment. «Time and Matter 2010», for Crystal Ball Collaboration at MAMI, Ivan Budva, Montenegro, October 4-8, 2010 Supek (University of Mainz for EU program 5. Krajcar Bronić I, Radiocarbon dating and its I3HP TNA Contract) application to early Neolithic in Croatia, 2nd + + 15. Precise Measurement of the Π → e n Balkan Symposium in Archaeometry - Sci- Branching Ration, Ivan Supek (International ence meets archaeology and art history. Collaboration between RBI and University of Kultur University, Istanbul, Istanbul, Turkey, Virginia, Charlottesville, USA) September 15-17, 2010. 16. OPERA collaboration, Ante Ljubičić (Inter- 6. Krajcar Bronić I, Environmental 14C Activity: RBI Annual Report 2010. RBI national collaboration between RBI, CERN The Atmosphere and The Biosphere, Scien- (Switzerland) and LNGS (Gran Sasso, Italy)) tific symposium “Public health research on

22 exposure to electromagnetic radiation”, Za- the RBI Laboratory for High Energy Physics, greb, 19 November 2010. RBI, November 15-19 2010. 7. Švarc A, Poles of PWData and PWAmpli- 4. IAEA Regional Workshop on ‘’Successful

tudes in Zagreb model. EBAC workshop on Applying for EU-funded projects’’, organized ZEF «Extractions and interpretations of hadron by the RBI (Stjepko Fazinić) in collaboration resonances and multi-meson production re- with the IAEA, Zagreb, 25-28 May 2010. actions with 12 GeV upgrade», Jlab, Newport 5. 14th European X-Ray Spectrometry Confer- News, USA, May 27 - 28, 2010, ence (EXRS 2010), 20-25 June 2010, Figuei- 8. Basrak Z, Equation of state of asymmetricic ra da Foz, Portugal, (Stjepko Fazinić member nuclear matter at supra-saturation densitie. of International Advisory Board) CBM collaboration meeting, Darmstadt, Ger- many, April 12-16, 2010 9. Szilner S, Quasi-elastic reactions: an inter- Selected publications play of reaction dynamics and nuclear structure. International Symposium on Quasi- 1. Karlušić M, Akcöltekin S, Osmani O, Monnet fission Process in Heavy Ion Reactions, I, Lebius H, Jakšić M, Schleberger M. Ener- Messina, Italy, November 8-9, 2010 gy threshold for the creation of nanodots on 10. Brigljević V, Low Pt Physics with CMS, LHC SrTiO3 by swift heavy ions. New J Phys 12 Days in Split, Split (Croatia), October 4-9, 2010. (2010), 043009-1. 11. Brigljević V, CP Violation, Lectures at the Sara- 2. Pinto S, Rolo A, Gomes M, Ivanda M, jevo School for High Energy Physics, Sarajevo Bogdanović-Radović I, Grenzer J, Mücklich (Bosnia and Hercegovina), May 10-12, 2010. A, Barber D, Bernstorff S, Buljan M. Forma- 12. Morović S, CMS Online Data Quality Monitor- tion of void lattice after annealing of Ge quan- ing: Real-Time Event Processing Infrastructure. tum dot lattice in alumina matrix. Appl Phys International Conference on Computing in High Lett 95 (2010), 173113. Energy and Nuclear Physics. Academia Sinica, 3. Agafonova N, et al. (OPERA Collabora- Taipei (Taiwan). October 18-22 2010. tion-RBI: Jakovčić K, Kliček B, Ljubičić A, Stipčević M). Observation of a first ντ candidate event in the OPERA experiment in the SELECTED ORGANIZED CNGS beam. Phys Lett B 691 (2010), 138. CONFERENCES 4. Andriamonje S, et al. (CAST Collaboration- RBI: Jakovčić K, Krčmar M, Lakić B, Ljubičić 1. New detector technologies for advanced A). Search for solar axion emission from 7Li materials research using ion beam analysis, and D(p,γ)3He nuclear decays with the CAST FP7 project SPIRIT Workshop organized by gamma-ray calorimeter. J Cosmol Astropart the RBI (chaired by Milko Jakšić, program or- P 3 (2010), 032-1.

ganized by Iva Bogdanović Radović, Stjepko 5. Batinić M, Ceci S, Švarc A, Zauner B. Poles RBI Annual Report 2010. Fazinić and Milko Jakšić), Plitvice lakes, Cro- of the Zagreb analysis partial-wave T matri- atia, 24.10.-27.10.2010 ces. Phys Rev C 82 (2010), 038203-1. 2. Origin of the Elements and Nuclear History 6. Tiator L, Kamalov S, Ceci S, Chen G Y, of the Universe, The first EuroGENESIS Drechsel D, Švarc A, Yang S N. Singularity workshop, organized by the RBI Laboratory structure of the PiN scattering amplitude in a for Nuclear Physics, chaired by Neven Soić, meson-exchange model up to energies W < Dubrovnik, 23- 26.11.2010. From the RBI Ne- 2.0 GeV. Phys Rev C 82 (2010), 055203-1. ven Soić, Vedrana Tokić and Lovro Prepolec 7. McNicoll E et al., (Crystal Ball Collaboration- participated in the organization. RBI: Korolija M, Mekterović D, Mićanović S, 3. Workshop on the first W and Z measure- Supek I, Zamboni I. Study of the gamma p -> ments with CMS at the LHC, organized by eta p reaction with the Crystal Ball detector at

23 the Mainz Microtron (MAMI-C). Phys Rev C rolija M). Measurement of K*(892)0 and K0 82 (2010), 035208. mesons in Al plus Al collisions at 1.9A GeV. 8. Prakhov S et al. (RBI: Supek I). Measure- Phys Rev C 81 (2010), 061902(R).

ZEF ment of K- p radiative capture to gamma 13. Mardanpour, H et al. (BINA Collaboration- Lambda and gamma Sigma0 for p(K-) be- RBI: Kiš M). Spin–isospin selectivity in three- tween 514 and 750 MeV/c. Phys Rev C 82 nucleon forces. Phys Lett B 687 (2010), 149. (2010), 015201. 14. R. Horvat: Entanglement in holographic dark 9. Di Pietro A et al. (RBI: Zadro M). Elastic Scat- energy models. Phys Lett B 693 (2010), 596. tering and Reaction Mechanisms of the Halo 15. Krajcar Bronić I, et al. A new graphite prepa- Nucleus 11Be around the Coulomb Barrier. ration line for AMS 14C dating in the Zagreb Phys Rev Lett 105 (2010) 022701. Radiocarbon Laboratory. Nucl Instr and Meth 10. Freer M et al. (RBI: Soić N). Cluster States B 268 (2010) 943. in 12C and 14C. Mod Phys Lett A 25 (2010), 1833. Books 11. Jiang C L et al. (PRISMA Collaboration-RBI: Szilner S). Fusion hindrance for Ca+Ca sys- 1. Obhođaš J, Valković V, Kutle A. Atlas of tems: Influence of neutron excess. Phys Rev Sediments (Croatia’s Coastal Region and Is- C 82 (2010); 041601(R). lands), In Croatian, Udruga Lijepa naša, Za- 12. Lopez, X et al. (FOPI Collaboration-RBI: greb, 2010. pp 231. ISBN: 978-953-97044- Basrak Z, Čaplar R, Gašparić I, Kiš M, Ko- 3-6. RBI Annual Report 2010. RBI

24 O of intensiveresearch. to bethesubjects systems continue densed in con- self-organization and ter interaction, effectswhile stronglynonlinear in laser-mat- spectroscopy inawide rangeofsystems, ics placed specialemphasisonvibrational in the field of molecular and solid state phys- ments intechnology. Fundamental research fundamental scientific research and develop has beenthemostactivedirectionofboth and nanotechnology ecules. Nanoscience matter andmol- properties of condensed and macroscopic mesoscopic microscopic, andconnectthe processes whichdescribe applied studiesof physical parametersand search. Weand arefocusedonfundamental - physics, re- chemistry andinterdisciplinary research inthefundamentalnaturalsciences frontier through of newknowledge mulation accu- and acquisition the is Physics terials verview oftheDivision Division ofMaterialsPhysics The primary mission of the Division of Ma- The primarymissionoftheDivision a a a laboratories: (ZFM) consistsofthefollowing The DivisionofMaterialsPhysics Head: NikolaRadić organization Division Physics, KrešimirFurić Laboratory forMolecular Nikola Radić Laboratory for Thin Films, Branko Pivac Laboratory for AL Semiconductors, - Al ces, where the ordering is achieved in asin- is achieved ces, wheretheordering ordered nanoclustersin amorphousmatri- nanoclusters onrippledsubstrates ordered spatially Growth of Carlo model(Buljanetal.,2010b). Monte- kinetic a by simulated and explained is dots quantum the of self-assembly served n h suy f e D ebde i SiO in embedded QDs Ge of study the in Deep Level Transient Spectroscopy(DLTS) Ge QDsfor non-volatile memories T tum dotsinasingle,600nmthick(Ge+Al material consisting of self-assembled Ge quan- dots inanaluminamatrix Gequantum Self-assembling of (Buljan etal.,2010a). demonstrated been in GeQDshas trapping sputtering. Charge by magnetron deposited op achievements 2 O A methodforthefabricationofspatially The successful application and analysisof The successful application The structuraldescriptionofanewnano- 3 film has been demonstrated. Theob- demonstrated. been has film http://www.irb.hr/en/zfm 2 O 3 )/ 2

25 2010. Report Annual RBI ZFM 26 RBI Annual Report 2010. ZFM rprto o hg qaiy odpd HfO Co-doped quality high of preparation This approachcaneasilybeextendedtothe chlorides withbenzylalcoholwasdescribed. metal thermal reactionofthecorresponding solvo- the on based route non- sol-gel aqueous a using nanocrystals oxide tantalum noparticles ytei o cytlie Nb crystalline of surfactant-free synthesis and Solvothermal substrate (Buljanetal.,2010b,c). of the the morphology following self-arrange, thenanoclusters ture. Duringthedeposition, substrate atanelevatedtempera- tion of a rippled multilayer ona periodically by thedeposi ordering isinduced Regular has beendemonstrated. large domain gle Ta netic ions(Buhaetal.,2010). distributionof with ahomogenous the mag - of uniformsizeandshape nanoparticles um oxideand(f)tantalumoxide. of theparticlemarkedwithanarrow (b)niobiumoxide,(d)hafni- (c) HfO2,(e) Ta2O5. HRTEM imageswiththerespectivePS(inset) ticles obtainedfromhalideprecursors inbenzylalcohol:(a)Nb2O5, Fig. 1. TEM overviewimagesofaspreparedmetaloxidenanopar- A simple route to hafnium and niobium, 2 O 5 , HfO , 2 , and Co-doped HfO Co-doped and , 2 2 na- O 5 2 - ,

Niobium DopedTiO TiO ofniobium-doped properties electrochemical for LithiumInsertion andItsApplication Mesoporosity 100 cycles(Wang etal.,2010). elevated acidconcentration inhibitsthereac- TSAkept low,was concentration an while of thep-TSAwhen thep- waspronounced chitectures) wasstudied. The catalyticrole ar- cauliflower complex, to bullet-like (from differentshapes nm) and to 250 sizes(20 with particles ZnO of formation the for agent as acatalystand/ormorphology-directing Morphologies Different with ZnOParticles Formation of p-Toluenesulfonic Acidinthe DoubleRole of The ble capacityof 160 mA h g properties. very good revealed Asta- highly and examined were performance cycle and cyclic voltammetry,properties, discharge battery,terials for a lithium-ion whose charge samples were used aspositiveelectrodema- face areawas described. The as-prepared C/4, C/2,1C,and2C. mance (c)ofthemesoporousNTO electrodecycledatC/8,C/6, C/6 uptothehundredthcycle(b),andcyclingrateperfor Coulombic efficiencybetween1and3.1Vvoltagewindow for thefirstcycle(a),discharge/chargecapacityprofileand Fig. 2. The galvanostaticvoltageprofilesbetween1and3.1V The synthesisaswellastudyofthe The role of p-toluenesulfonic acid (p-TSA) acid of p-toluenesulfonic The role 2 (NTO) with mesoporosity and high sur- high and mesoporosity with (NTO) 2 with -1 was foundafter - nanorods preparedwithouttheassistanceofp-TSA. of Fig.1(c)isshownintheinset.(d)HRTEM imageof theZnO ing powerspectrumofthenanoroddisplayedinupperpart HRTEM imageofZnOnanorodsdescribedin(a). The correspond- of ZnOnanorodspreparedwithouttheassistancep-TSA.(c) the assistanceofp-TSA (0.02mmol,5h).(b)Overview TEM image Fig. 3.(a)Overview TEM imageofZnO nanorodspreparedwith 2. 1. Education plex architectures(Ambrožićetal.,2010). self-assembly of ZnO nanocrystals into com- ZnO particle growth and shape as well as the withthe p-TSAsurface chelation controls the p-TSA on its surface. The abundance of ZnO of the lysis, presumablyduetotheadhesion tivity of the ZnO precursor toward the alcoho Research programs Zagreb andelsewhere. and Engineering of Chemical Technologyin cal Engineering and Computing, the Faculty of Sciences, Faculty of topics at uate coursesinphysicsandrelated and 4post-grad Physics teach10graduate Members of the Division of Materials Branko Pivac and dielectrics, fects insemiconductors de- and of nanostructures properties Basic ites, Aleksandra Turković and nanocompos Synergy ofnanophases Electri- Faculty - - - 8. 7. 6. 5. 4. 3. 1. Science,Education andSports of bytheMinistry supported Program 7. 6. 5. 4. 3. 2. 1. projects international Research, developmental and Stjepan Lugomer molecular systems, actions incondensed inter- optical and processes Organizational and bulkmatter of nanostructures Physics andapplication and nanotechnology materials foroptoelectronics Semiconductor structured Materials Thin FilmsofNovel or Nano Amorphous to crystallinetransition,DavorGracin amorphous the on alloys silicon film thin The terials, BiserkaGržeta Doped optoelectronicandceramicnanoma ergy conversionandstorage,BrankoPivac for en- and applications materials Advanced 30.09.2011) Young Researchers Program; 01.10.2009 - – Fund Knowledge Through Unity ties andcharacterization ((I.Capan,PI, Silicon nanocrystalssolarcells...Proper- logical Cooperation) Techno and Scientific Chinese - (Croatian (B. Pivac, PI) defects insinglecrystalsilicon structural and precipitation oxygen of Study project 2009-2010) bilateral Croatian-French (COGITO PI) pan, in Germanium(I.Ca- of doping acterization char- electrical and tomography Atom probe Stjepan Lugomer(Croatia-Hungary) mation ofsurfaceswithspecialproperties, for- surface self-organization: Laser-induced Croatian-Slovenian bilateralproject) (D.Gracin, cells solar of generation” “third for Nanocrystalline silicon as a possible candidate (D.Gracin, Croatian-Slovenianbilateralproject) onvibra- tional propertiesinnanocrystallinesilicon) confinement quantum of Influence venian bilateralproject2009-2010) (B. Pivac,PI)(Croatian-Slo by fastneutrons irradiated Study ofdefectsinsemiconductors , KrešimirFurić , NikolaRadić , BrankoŠantić - - - -

27 2010. Report Annual RBI ZFM 8. The origin of structural defects in silicate 5. Buljan M, Bogdanović Radović I, Karlušić glasses and their effects upon the properties, M, Desnica U, Radić N, Skukan N, Dražić Davor Gracin, (National Science Foundation G, Ivanda M, Gamulin O, Matej Z, Valeš V,

ZFM of Croatia) Grenzer J,Cornelius T, Metzger HT Holy V. Generation of an ordered Ge quantum dot ar- Awards ray in an amorphous silica matrix by ion beam irradiation. Phys Rev B 81 (2010c) 085321. Dr.sc. Maja Buljan won the Annual RBI 6. Buljan M, Grenzer J, Keller A, Radić N, Valeš Director’s Award for excellence in scienctific V, Bernstorff S, Cornelius T,Metzger HT, Holy research and publishing in highly rated sci- V. Growth of spatially ordered Ge nanoclus- entific journals. ters in an amorphous matrix on rippled sub- Dr.sc. Davor Gracin and Dr.sc. Branko strates. Phys Rev B 82 (2010d) 125316. Pivac, respectively, won the Annual RBI Di- 7. Gupta SK,Desai R, Jha PK, Sahoo S, Kirin D. rector’s Award as recipients of competitive Titanium dioxide synthesized using titanium grants (greater than 70.000 EUR). chloride: size effect study using Raman spectroscopy and photoluminescence. J Raman selected organized Spect 41 (2010) 350. conferences 8. Kaper H, Sallard S, Djerdj I, Antonietti M, Smarsly BM. Toward a Low-Temperature Maja Buljan received the RBI Director’s Sol-Gel Synthesis of TiO2(B) Using Mixtures Award for scientific excellence in 2010. Da- of Surfactants and Ionic Liquids. Chem Mater vor Gracin and Branko Pivac received the 22 (2010) 3502. RBI Director’s Award as recipients of com- 9. Pinto SRC, Rolo A, Gomes MJM, Ivanda M, petitive grants (greater than 70.000 EUR). Bogdanović-Radović I, Grenzer J Mucklich A, Barber DJ, Bernstorff S, Buljan M. Formation Selected publications of void lattice after annealing of Ge quantum dot lattice in alumina matrix. Appl Phys Let 1. Ambrožič G, Djerdj I, Škapin S, Žigon M, Crn- 97 (2010) 173113. jak Orel Z. The Double Role of p-Toluenesul- 10. Stipcevic M, Skenderovic H, Gracin D. Char- fonic Acid in the Formation of ZnO Particles acterization of a novel avalanche photodiode with Different Morphologies. CrystEngComm for single photon detection in VIS-NIR range. 12 (2010) 1862. Opt Express 18 (2010) 17448. 2. Buha J; Arcon D; Niederberger M; Djerdj I. 11. Wang YD, Smarsly BM, Djerdj I. Niobium Solvothermal and surfactant-free synthe- Doped TiO2 with Mesoporosity and its appli- sis of crystalline Nb2O5, Ta2O5, HfO2, and cation for lithium insertion. Chem Mater 22 Co-doped HfO2 nanoparticles. Phys Chem (2010); 6624. Chem Phys 12 (2010) 15537. 12. Zhang J, Su DS, Blume R, Schloegl R, Wang 3. Buljan M, Grenzer J, Holy V, Radić N, Mišić- R, Yang X, Gajovic A. Surface Chemistry and Radić T, Levichev S, Bernstorff S, Pivac B, Catalytic Reactivity of a Nanodiamond in the Capan I. Structural and charge trapping Steam-Free Dehydrogenation of Ethylben- properties of two bilayer (Ge+SiO2)/SiO2 zene. Angewandte Chem - Int Ed 49 (2010) films deposited on rippled substrate. Appl 8640-8644 Phys Lett 97 (2010a) 163117. 4. Buljan M, Pinto SRC, Rolo AG, Mar- Selected invited lectures tin-Sanchez J, Gomes MJM, Grenzer J,Muecklich A, Bernstorff S, Holy V. Self-as- 1. Buljan M, Desnica UV, Radić N, Pivac B, RBI Annual Report 2010. RBI sembling of Ge quantum dots in an alumina Dražić G, Bernstorff S, Corenlius T, Metzger matrix . Phys Rev B 82 (2010b) 235407. H, Holy V.Production and design of regularly

28 ordered quantum dot lattice in amorphous 3. Radić N. C/W nanolaminates - alternating matrices. 13th Joint Vacuum Conference, various C & W phases. E-MRS Spring Meet- Programme and Book of Abstracts, ed: Vese- ing, Symposium L: “Carbon- or Nitrogen-

ly, Marian; Vincze, Andrej; Vavra, Ivo. Štrbske containing Nanostructured Composite Films, ZFM Pleso, Slovačka, Slovak Vacuum Society, Strasbourg, France, June 7-11, 2010. 2010, 97-97, date is missing 4. Desnica U. Electricity from the Sun: A Bright 2. Turković A. SAXS Studies of TiO2 Nano- Future Shines on PV. 10th International Con- particles in Polymer Electrolytes, Second ference: “ECSAC 10 - Sustainable Energy: Regional Symposium on Electrochemistry, Challenges and Opportunities”, Lošinj, Croa- South-East Europe, Beograd, Srbija, June 6, tia, August, 23-27, 2010. 2010. Program & Book of Abstracts / Nikolić, 5. Desnica U. Sustainable Energy: Challenges Branislav ; Mišković-Stanković, Vesna, De- and Opportunities. 22nd Conference „Ener- kanski, Aleksandar (ed.). -Beograd: Serbian gy and Environment 2010”,Opatija, Croatia, Chemical Society, 2010. 15-15. October, 19-23, 2010. RBI Annual Report 2010.

29 RBI Annual Report 2010. RBI

30 O age deconvolution and blind decomposition age deconvolutionand blind decomposition sional signalswithapplications inblindim- approach toblindseparation ofmultidimen- in chemometrics,useofa tensorfactorization sparse componentanalysis withapplications ers, blind and semi-blind signal processing, zation and modelling of very thin metallic lay- terials inopticalmultilayerdesign,characteri- metallic nanoclusters,useofplasmonicma- theories, researchonplasmonicpropertiesof medium effective using mixtures films thin of al strategic projects. These include modelling to thefieldofnationalsecurity. disciplines basic of these application to the dition, weareinvolvedinactivitiesdirected ad- In films. thin optical of fundamentals the and optics, photonics non-imaging and aging im- of field the in knowledge strengthen and verview oftheDivision Research andDevelopment Division ofLaserand consists ofthefollowinglaboratories: of Laserand The Division Atomic R&D Head: HrvojeZorc DIVISIONAL organization The Divisioniscurrentlydevelopingsever- expand to is Division the of mission The a a Films, Vesna Janicki Laboratory ofOpticsand Thin Eduard Švegel Eduard workshops, Multipurpose increases asthe particle sizereducesdueto enables structuringofsuch films. temperature increased and field electric of clusters, involvinginstantaneous application tric field assisted dissolution (EFAD) of metal Elec- cooperation. of international framework (AFM, GISAXS, RBS, ellipsometry), inthe zation are carried out by different techniques of the films. Structural and optical characteri properties of optical tuning varied, enabling properties oftheclusterscanbe geometrical and structural the films, these of preparation in the metal clusters.Byvaryingconditions plasmon resonance(SPR)of electrons in surface the to due properties optical unique (MIF) and their applications. Such films show plasmonic properties of the metal island films Optical thinfilms TOP ACHIEVEMENTS resentation ofsignals. rep- (sparse) efficient for algorithms learning of multispectral/hyperspectral images; basis The widthoftheSPR metalparticles Weon the withoutresearch continue Atomic http://www.irb.hr/en/zlair -

31 2010. Report Annual RBI LAIR 32 RBI Annual Report 2010. LAIR lective EFADin ofmetalclustersembedded by thelocallyse- structures areproduced the sample. tuning oftheopticalproperties of enabling are changed, shape line and tion intensity cles size. Thus, the surface plasmonabsorp parameters allowscontrolofthenanoparti annealing thermal of Variation dissolution. film metal of parameters the of modification by tailored depth be might particles metal of profile the that suggest results The matrix. embedded inaglass of metal nanoparticles thermal annealing,resultsinthe formation limit requiredbyclassicalMietheory. cal extinction spectroscopy beyond the dilute ful in extending nanoparticle sizing from opti- of afewpercent. These resultscanbeuse- be neglectedevenatvolumeconcentrations interaction toSPRwidthcannot interparticle of metals thecontribution (1) thatfornoble ly related to the plasmonwidth.It is shown cle concentration,particlesizecanbedirect- electric function.In the limitof very lowparti- effects di- confinement metal the modify that the electroderotated90◦insecond treatment(c). multilayer sampleaftertwoconsecutive EFAD treatments,with electrode (a),multilayersampleafter EFAD process(b)and Fig. 1:Opticalmicroscopypicturesof thegratingusedas Three dimensional (3D) photonic micro- (3D) photonic Three dimensional of EFADApplication toMIF,by followed - - posed methodology is of widespread signifi widespread of is methodology posed spectrometry.pro- mass The using mixtures two from analytes five and spectra) (COSY spectroscopy NMR 2D using from mixtures three analytes four of extraction blind a on exemplified is method The mixtures. of ber num- the than be greater or to equal can than, less analytes of number The mixtures. the analytesthemselves,from available and profiles concentration their of matrix the of estimating:the numberofanalytes, pable position schemes,thepresentconceptisca- decom- blind existing many to opposed As 2010). Jerić, and (Kopriva measurements and spectrometric types ofspectroscopic various in recorded mixtures from analytes of to blindseparation and applied veloped multivariate dataanalysismethodwasde- Signal processing metallodielectric photonicstructures. duction ofnovelcomponentsbasedon3D- represents apromisingmethodfor the pro- structures, thecurrent approach resulting of the properties modify theoptical widely to and thepossibility 2). Duetoitsfeasibility SPR of non dissolvedmetal clusters (Figure ferential effects of multilayer stacks and the the inter- that combines optical behaviour produced structures show ahighlytailorable 1). stacks (Figure multilayer dielectric The a multilayerof Ag andSiO2films. and (b)multilayerstructureBK7/(X/SiO2)5,inwhichXrepresents BK7/(Ag/SiO2)5 withthicknessof7nm Ag and100nmofSiO2 structures producedbyEFAD treatment:(a)multilayerstructure Fig. 2Wavelength-dependence ofdiffraction pattern ofmultilayer Asparseness-based novel conceptually - n, o-eaie arx atrzto, ICA factorization, matrix non-negative ing, tive contours(levelset), K-meanscluster- image segmentationmethods suchasac- performance analysis with state-of-the-art the surroundingtissue.Bycomparative and spectral spatial diversities between the BCC and exploit they since gorithms, is due to thescaleinvariancepropertyofDCA al- which fluctuation intensity against robustness exhibits method The 2010. 18, Photochem. Photobiol. B, vol. 100, pp. 10- is presented in: I. Kopriva the BCC of image fluorescent (RGB) blue pervised decomposition of the red-green- basal cellcarcinoma(BCC)throughunsu- (DCA)-based approachtodemarcationof about parametricmodeloftheblur. however,which, requires algorithm tion totheblindRichardson-Lucy better performanceisdemonstratedinrela- or comparable A method. (HOOI) iteration gorithm knownashigherorderorthogonal al- SVD multilinear the of means by plished (photon sieve). a grating This isaccom- and turbulence by: defocusing,atmospheric blurred images multispectral ic anddynamic stat- and multiframe on single- demonstrated 17833, 2010, . The method is experimentally 17819- pp. 18, vol. Express Optics Kopriva, in: I. is developed scenario blind completely An imagerestorationmethodthatworksina PSF is space invariant withaknownmodel. rely on known. The majority of existing BID methods is un- medium (PSF) ofthe function spread that point provided its blurredobservation ofrestoringtheimagefrom to theproblem regulating networks. construction offactors in gene transcription such asthere- problems insystemsbiology to solveimportant method canbeapplied blood, plasma,etc.Moreover, theproposed urine, as such fluids biological from isolated studies, wherein mixtures represent samples products research and the field of metabolic natural being cance withthemainapplication A noveldependent component analysis (BID) refers deconvolution image Blind information and assume that a and assume a prioriinformation a prioriinformation et al., Journal. 3. 2. 1. Education 1. projects international Research, developmental and 2. 1. Science,Education andSports of bytheMinistry supported Projects projects AWARDS magnitude. age has been varied almost two-orders of narios where intensity of the fluorescent im- BCC demarcation in two demanding sce- onstrate goodperformanceofDCA-based dem- experimentally we imaging ratio and financed bytheEuropeanUnion. Award asarecipientofanIPA projectgrant tific excellencein2010. received theRBI Director’s Award for scien- Hrvoje Zorc received the RBI Director’s Hrvoje Zorc received theRBI Director’s Ivica KoprivaandJordiSancho-Parramon Velika in Gorica(Lončarić,Martin). College Polytechnic Optics, Ophthalmic of Study Optics, Geometrical of Laboratory Gorica (Lončarić,Martin). Velikain College Polytechnic Optics, thalmic Oph- of Study Optics, Physical of Laboratory University ofZagreb(Kopriva,Ivica). and Computing, ulty ofElectricalEngineering component analysis,graduatecourse,Fac- and independent separation Blind signal terials andPhotonics,Weiz, Austria, ZorcH. laboration withInstituteofNanostructured Ma- bedded indielectricmultilayers, Projectincol- em - nanoparticles metal of properties Optical Hrvoje Zorc layers, nanostructured of properties Optical Analysis ofmultispectraldata,IvicaKopriva

33 2010. Report Annual RBI LAIR 2. Collaboration in development of optical sys- glass matrix formed by thermal annealing of tems for the company HS Produkt, Croatia field-assisted film dissolution. Opt Mater 32 3. Collaboration with Dr. Salvador Bosch (Uni- (2010) 510.

LAIR versity of Barcelona) on Mueller matrix prop- 3. Janicki Vet. al., Three-dimensional photonic erties of single particle scattering microstructures produced by electric field as- 4. Collaboration on algorithms for nonnegative sisted dissolution of metal nanoclusters in matrix and tensor factorizations and their ap- multilayer stacks. Appl Physics B - Laser Opt plications in chemometrics and multispec- 89 (2010) 93. tral image analysis with Professor Andrzej 4. Kopriva I, Jerić I (2010). Blind separation Cichocki (Laboratory for Advanced Brain of analytes in nuclear magnetic resonance Signal Processing, Brain Science Institute, spectroscopy and mass spectrometry: RIKEN, Japan). sparseness-based robust multicomponent analysis. Anal Chem 82 (2010) 1911. 5. 5. Kopriva I, Peršin A, Puizina-Ivić N, L. Mirić Selected INVITED lectures L. Robust demarcation of basal cell carcinoma by dependent component analysis-based 1. I. Kopriva. Tensor Factorization Approach to segmentation of multi-spectral fluorescence Blind Separation of Multidimensional Sourc- image. J Photochem Photobiol B - Biol 100 es, Virginia Comonwealth University, Depart- (2010) 10. ment of Computer Science, Richmond, VA, 6. Kopriva I. Tensor Factorization for model- USA, February 17, 2010. free space-variant blind deconvolution of the 2. I. Kopriva. Tensor Factorization Approach to single- and multi-frame multi-spectral Image, Blind Separation of Multidimensional Sources, Opt Express 18 (2010) 17819. The George Washington University, School of Engineering and Applied Science, Depart- ment of Electrical and Computer Engineering, PEER REVIEWED CONFERENCE Washington D.C., USA, February 19, 2010. PAPERS 3. The paper: I. Kopriva, I. Jerić (2009). Multi- component Analysis: Blind Extraction of Pure 1. I. Kopriva, A. Peršin (2010). 3D tensor-based Components Mass Spectra using Sparse blind multi-spectral image decomposition for Component Analysis, Journal of Mass Spec- tumor demarcation, accepted for SPIE Medi- trometry, vol. 44, issue 9, pp. 1378-1388, has cal Imaging-Image Processing, San Diego, been selected as one of the most novel and CA, USA, February 13-18, 2010. groundbreaking contributions in the field of 2. I. Kopriva, A. Cichocki (2010). Nonlinear chemometrics in the years 2008 and 2009 Band Expansion and 3D Nonnegative Ten- in a review article: B. Lavine, J. Workman sor Factorization for Blind Decomposition (2010). Chemometrics, Analytical Chemistry, of Magnetic Resonance Image of the Brain. vol. 82, pp. 4699-4711. Proceedings of 9th International Conference on Latent Variable Analysis and Signal Sep- aration, Lecture Notes Computer Science Selected publications 6365, pp. 490-497, V. Vigneron (editor), Sep- tember 27-30, 2010, Saint Malo, France. 1. Sancho-Parramon J, Janicki V, Zorc H. On 3. META 10, 2nd International Conference on Met- the dielectric function tuning of random metal- amaterials, Photonic Crystals and Plasmonics, dielectric nanocomposites for metamaterial Multilayer based interferential-plasmonic struc- applications, Opts Express 18 (2010) 26915. ture: metal cluster 3D grating combined with RBI Annual Report 2010. RBI 2. Sancho-Parramon J et. al. Optical and struc- dielectric mirror 22-25 February 2010. Cairo, tural properties of silver nanoparticles in Egypt, V. Janicki, J. Sancho-Parramon,

34 4. META 10, 2nd International Conference on 5. OIC - Optical Interference Coatings 2010, Metamaterials, Photonic Crystals and Plas- Tucson USA, V. Janicki, J. Sancho-Parramon, monics, 22-25 February 2010. Cairo, Egypt, H. Zorc, Gradient silver nanoparticle layers in

J. Sancho-Parramon, V. Janicki, M. Lončarić, absorbing coatings - experimental study LAIR H. Zorc, P. Dubček, S. Bernstorff, Optical and 6. OIC - Optical Interference Coatings 2010, structural properties of silver-gold islands Tucson USA, H. Zorc, M. Lončarić, J. Sancho- films for plasmonic application, Parramon, V. Janicki, Use of Gold Island Films in Design of Reflectors with High Luminosity RBI Annual Report 2010.

35 RBI Annual Report 2010. RBI

36 O tion and Sport, in 2010 the Division obtained tion and Sport, in 2010 the Division obtained supported bytheMinistry ofScience,Educa- courses inbioinformatics andbiomedicine. courses andPhD of graduateengineering a number through the UniversityofZagreb the graduateandpost-graduatecurriculaof to significantly contribute members Division record. Senior and publications laborations of our col- character in themulti-disciplinary reflected is which economics, and neering computational butalsoinengi bioinformatics, and biology biomedicine, in techniques of these applications is placedontailored Emphasis analysis. signal and techniques measurement tion systems)withadvanced for informa- representations ods, knowledge learning andintelligentdata analysis meth- (machine intelligence artificial of fields the in research of Electronicscombines sion

verview oftheDivision DIVISIONAL ORGANIZATION Division ofElectronics In addition to long term research projects In additiontolongterm research projects in theDivi The maintopicsofresearch a a a two laboratoriesandonegroup: The Divisionofelectronicsconsists Head: TomislavŠmuc

bioinformatics group, Tomislav Šmuc and biology Computational Rogina Medved Branka research, and processes forstochasticsignals Laboratory systems, DraganGamberger forinformation Laboratory - - focused in two different application domains; mental demonstration of this new extension is Experi- tools. meta-learning and planning via age RapidMinerandtheir jointenactment Taverna, withopensourcedatamining pack- of e-science computational infrastructure like positories. This project represents a coupling mendation servicesfordigitalmultimediare- work package on Personalization and recom- Sciences; FP7STREP),leadingactivitiesina Research inDataMiningandData-Intensive Laboratory for Interdisciplinary Collaborative (e- e-LICO project EU the on work began ics ICO project ICO L e- computer science Knowledge technologies and T V. Radeka, B. Souček, L. Budin and B. Jeren. presented byformerRBIprominentscientists computing attheInstitutewithinvitedtalks the celebration of 60 years of electronics and dation researchgrant.In2010weorganized student received a Croatian Science Foun- PhD one and two theses PhD their year finished students that During project. COST one op achievements On June 1 and one EU-FP7 Collaborative Project and one st , 2010theDivisionofElectron- http://www.irb.hr/en/zel

37 2010. Report Annual RBI ZEL 38 RBI Annual Report 2010. ZEL rogate model (Fig. 2.) of a computationally rogate model (Fig. 2.) of a computationally rithm isdemonstratedby constructingasur- algo- the of efficiency The computers. digital in implement to simple are and complexities achieve high approximation accuracies at low organized polynomialneuralnetworksmay cedures in real-time measurements. The self- pro- complex of calculation the modelling for type polynomialneuralnetworksisadapted mensional systemsbyself-organizingGMDH- applications real-time for complexAdapting procedures systems techniques andmeasurement Advanced signalprocessing pek etal.,2010). tically universal,asdepictedinFig.1.(Su- is prac- prokaryotes in selection translational that evidence has shown method our field, this in results state-of-the-art Unlike index. adaptation such asthecodon approaches, performs previousdistancemeasure-based is practically universal. This approach out- that translational selectioninprokaryotes genomes); our method has shown evidence prokaryotic examined (461 date to study ic genom In thelargest organisms. prokaryotic effectsselection ongenesof of translational detects classifier Forest Random on based or accuratetranslation. Aapproach novel wards codons that enable more efficient and/ mayshowa preferenceto- pressed genes ex- highly but DNA, in patterns substitution background of consequence a largely is es approach translation usingmachine learning genesoptimized for Detection of systems. tal multimedia repositories and recommender digi- of field the in is members) DEL by (lead one of these domains is biomedical, the other The algorithm for approximation of multidi- of approximation for algorithm The biasinprokaryotic genom usage Codon - - (Marić andIvek,2010). using low-computingpowermicrocomputer tion ofthermodynamiceffects inreal-time procedure, whichenablesdirectcompensa- correction error flowrate gas natural intensive umn have inputs that are mutually independ- straints require it. All elements in the same col- because it can be pipelined should timing con- used becauseitallows parallelism, andalso is structure tree The 3.). (Fig. figure on shown value isperformedusingminimumsearchtree video capturesystem. A searchfortheminimal various video signal filters into an FPGA based camera). We havedevelopedand integrated to beembeddedintothecameraitself(smart tion anddecisiononaction(e.g.raisingalarm) high computerpowertoenableeventrecogni- need of rehabilitation. Such systems demand people withdisabilities,andthosewhoarein everyday living activities of elderly people, AAL systemthatsupervisesandassists the of thefocuseswasavideomonitoringbased One applications. (AAL) living assisted bient ble gatearray(FPGA)devicesforuseinam- we evaluated the potential of field programma - applications for ambientassistedliving systems FPGA basedembedded genomes. Fig. 1.Prevalenceofcodonusageoptimizedgenesinprokaryotic In collaboration with our Hungarian partners In collaborationwithourHungarianpartners subjects withHuntington disease (Fig.4.) data (strideintervals)for healthy subjectsand locomotion human on verified was method the publications (Michieli et al., 2010). Adequacy of several through established was that tification method for fractal features detection and quan- sity ofZagreb,resultsinintroductionanew tion withtheFacultyforKinesiology, Univer- collabora- in characterization, and extraction This workhasresultedinaPhDthesis. are manifestedinrestrictedjawkinematics. tification ofdifferent mandiblepathologies that the possibilityto use it for detection andquan- Resolution capabilityofthemethodindicates functions. coefficient wavelet designed cially spe- using identified clearly are movements data. Different positionsoftheheadduringjaw wavelet analysisofrespectiveaccelerometric terns ofmandibleopen-closingcyclesusing method forcapturingdifferent kinematicpat- adequate than more a is accelerometry that on mandible kinematics. The results show analysis ofhumansystemsmotionwefocused ing accelerometrybasedmeasurementsin human systemsmotion of inanalysis measurements based Accelerometry processing applications. been foundtobewellsuitedforsuchvideo have FPGAs parallel. in executed are and ent error correction. Fig. 2.Self-organizedpolynomialneuralnetworkforflowrate The research of human walk fractal features The researchofhumanwalkfractalfeatures us- of sufficiency the of investigation the In

Education 2. 1. projects international Research, developmental and 4. 3. 2. 1. Sports Sports Science,Education and of Ministry bythe Research supported projects students completedtheirPhDtheses. ofZagreb. of MedicinetheUniversity Two puting, the Faculty of Sciences andSchool and Com- Faculty ofElectricalEngineering courses inthe uate, graduateanddoctoral tinued to be involvedinlecturingundergrad Fig. 3.SchematicofanFPGA videocapturesystem In 2010, the members of the Division con- In 2010,themembersofDivision gramme), BrankaMedved Rogina S&TIntergovernmental atian-Hungarian Pro- for assistive applicationselderly people(Cro- based systems embedded Reconfigurable Project), ta-Intensive Science (EU-FP7 Collaborative Collaborative ResearchinDataMiningandDa- Interdisciplinary for e-Laboratory An e-LICO: Computational Biology, Tomislav Šmuc of Machine LearningPredictiveModelsin zation, BrankaMedvedRogina Real Life Data MeasurementandCharacteri urement Systems,IvanMarić Methods inMeas- Intelligence Computational cation, DraganGamberger Learning Machine and their Algorithms Appli Tomislav Šmuc,DraganGamberger - - -

39 2010. Report Annual RBI ZEL 3. Inductive Rule Learning (Croatian-Slovenian 2. Supek F, Šmuc T: On relevance of codon usage Intergovernmental S&T Programme), Dragan to expression of synthetic and natural genes in Gamberger Escherichia coli. Genetics 185 (2010) 1129. ZEL 4. The Emergence of Southern Multinationals 3. Gredičak M, Supek F, Kralj M, Majer Z, Hol- and their Impact on Europe (COST IS0905 losi M, Šmuc T, Mlinarić-Majerski K, Horvat Š. action), Dragan Gamberger Computational structure-activity study directs synthesis of novel antitumor enkephalin ana- Research programs supported by logs. Amino acids 38 (2010) 1185. the Ministry of Science, Education 4. Marić I, Ivek I: Compensation for Joule-Thom- and Sports son effect in flow rate measurements by GMDH polynomial. Flow Meas Instrum 21 (2010) 134. 1. Computational knowledge discovery in scien- 5. Michieli I, Medved Rogina B, Ristov S: Data tific applications, Dragan Gamberger series embedding and scale invariant statis- tics. Hum Movement Sci 29 (2010) 449. Awards Chapters in the books Ivan Marić, Tomislav Šmuc and Fran Su- pek received the RBI Director’s Award for 1. Lavrač N, Fuernkranz J, Gamberger D: Explic- scientific excellence and international pro- it Feature Construction and Manipulation for Covering Rule Learning. Studies in Compu- ject in the field of electronics in 2010. tational Intelligence. Koronacki J (ed). Berlin, Matko Bošnjak and Dragan Gamberger Springer-Verlag, 2010. pp. 121-146. (as a RandomGuy team) won second place 2. Daciuk, Jan; Piskorski, Jakub; Ristov, Stra- at RSCTC’2010 basic track of a discovery hil. NLP Dictionaries Implemented as FSAs. challenge. The challenge has been organ- Mathematics, Computing, Language, and ized as a part of The Seventh International Life: Frontiers in Mathematical Linguistics Conference on Rough Sets and Current and Language Theory - Vol. 2 Scientific Ap- Trends in Computing, about 80 teams took plications of Language Methods. Martín-Vide part from different institutions and the task C (ed), London: World Scientific & Imperial has been prediction of classes based on College Press, 2010. pp. 133-204. measured gene expression values. 3. Prcela M, Gamberger D, Šmuc T, Bogunović ORGANIZED CONFERENCE N: Information gain of structured medical diagnostic tests: Integration of Bayesian networks and ontologies. Proceedings of In- 60 years of electronics and computing at ternational Conference on Biomedical Engi- the Institute, Zagreb, Croatia, May 31, 2010. neering Systems and Technologies. Fred A, Karolj Skala and Tomislav Šmuc Filipe J, Gamboa H (eds). Portugal: Institute for Systems and Technologies of Informa- Selected publications tion, Control and Communication, 2010. pp 235-240. 1. Supek F, Škunca N, Repar J, Vlahoviček K, 4. Sluban B, Gamberger D, Lavrač N: Advances Šmuc T: Translational Selection Is Ubiqui- in class noise detection. Proc. of 19th Euro- tous in Prokaryotes. Plos Genetics 6 (2010) pean Conference on Artificial Intelligence, e1001004-1. ECAI 2010. Coelho H, Studer R, Wooldridge M (eds). 2010. pp. 1105-1106. RBI Annual Report 2010. RBI

40 O tides and proteins research, and inother tides andproteinsresearch, pep- processes, and chemical of physical al chemistry, theoretical chemistry, modeling chemistry,pheric kinetics, structur- chemical lished morethan50contributionsinatmos- molecular research. of areas selected in Excellence recognized Centerof as aninternationally and theoreticalchemistry, in ordertoemerge coordination science, chemistry, spectroscopy, andcomputational protein of fields the in knowledge of fundamental dissemination and discovery,exploitation, the is Chemistry verview oftheDivision Head: Aleksandar Sabljić DIVISIONAL ORGANIZATION In 2010. members of the Division pub- of Physical of theDivision The mission Division ofPhysicalChemistry a a a a a

of Organic Molecules, Ivan Habuš of OrganicMolecules,Ivan Processes ofSelfassembling Laboratory forSynthesisand Rakvin Boris Resonances, Laboratory forMagnetic Marija Luić Crystallography, Biological ofChemicaland Laboratory Bosanac Slobodan Group, Theoretical Chemistry Branka Kovač Chemistry, Atmospheric and forChemicalKinetics Laboratory Air ForceOfficeofScientific Research. shops has been supported by NATO and the reates. series ofwork- This highlyregarded lau- of Nobel theparticipation which included conference held international a traditionally FERENCE –SustainableSourcesof Energy members organized the 12 organized members Last butnotleast,division Croatia. in ucation ed- and post-graduate es) toundergraduate cours- 30 (about extensively contribute also Research European members Area. Division strate a strongpresence of the Divisionin demon collaborations fruitful international collaborations. and international A number of these publicationsresultedthroughdomestic Society forMassSpectrometry.can of Half CrystEngComm, andJournalof the Ameri- of Physical Chemistry A, ChemBioChem, cal ChemistryChemicalPhysics, Journal Physi- Dalton Transactions,Letters, Organic National Academy of Sciences oftheUSA, of the ing journalssuchas:Proceedings in thehighestrank - papers werepublished of number significant Abioscience. of areas http://www.irb.hr/en/zfk th BRIJUNI CON- BRIJUNI -

41 2010. Report Annual RBI ZFK 42 RBI Annual Report 2010. ZFK all three imidazoles to simultaneously bind to simultaneously three imidazoles all allow to designed been have linkers O-CH2- a seven-stepsyntheticprocedure. The CH2- through was prepared clear metalloenzymes, tion coreinthe active sites of many mononu coordina trihistidine ubiquitous the of model arms, representsastructuralbiomimetic three imidazolcoordination bearing ligand metalloenzyme active site themononucelar model of Abiomimetic Resorcin[4]arene: (Močibob etal.,2010). synthesis peptide nonribosomal dependent in- template and biosynthesis protein mal triguing linkbetweenprogrammableriboso synthesis, andthustheyrepresentanin- peptide nylation domainsinnonribosomal is reminiscentofade - aSerRS homologues instead oftRNA. The enzymaticactivityof carrier proteins tein] ligases,aminoacylate synthetases, namedaminoacid:[carrierpro- of discovered homologues aminoacyl-tRNA biosynthesis. Recently protein for ribosomal that areusedassubstrates noacyl-tRNAs enzymes thatcatalyzetheformationofami- conserved are ancientandevolutionarily peptide synthesis ribosomal andnonribosomal synthetases: alinkbetween aminoacyl-tRNA Homologs of T (left) andglycyl:[carrierprotein]ligase(right). Fig. 1.Structuralcomparisonofatypicalseryl-tRNA synthetase op achievements The bowl-shaped resorcin[4]arene-based The bowl-shaped Aminoacyl-tRNA synthetases(aaRSs) - - - solution andinthesolid-state. Fig 3. Azobenzene/bipyridine palladacycleasPd(II) emitterinthe same side of azobenzene ligand andsig- same sideof azobenzene of bothPdatomsontothe bles positioning ena- azobenzenes in doublecyclopalladated et al., 2010). The cleavageof one Pd-Nbond (Juribašić characterized fully and sized have beensynthe- C,N-donors bidentate as monodentateC-and act simultaneously in whichazobenzenes ridine palladacycles temperatures at ambient Pd(II) emitters bowl-shaped cavity(Višnjevac etal.,2010). the to relative positions exo and endo both at exchange and binding guest selective the of to becapable and shown were prepared plexes (perchlorato- and acetato-complexes) Zn(II) two mononuclear com- corresponding The ligands. external exchangeable two of a metalionandtofavorcis-coordination the endo-andexo-positionsinmodelcomplex. Fig. 2.Selectiveandreversiblebindingoftheguestmoleculesat The first examples of azobenzene/bipy examples first The - We have shown that in Ac-Val-Phe-NH in that shown have We I(g+) in Ac-Gly-Phe-NH I(g+) β-turn and γL(g-) γL- as conformers experimentally observed the identify to able were We local second-order Moller-Plesset method. the with performed was Gly,Ala = Xxx NH2, Ac-Xxx-Phe- peptides: small of preferences dipeptides protected in formation structure Secondary peratures. in solutionandsolidstateatambienttem- emitters Pd(II) of examples rare are plexes both solutionandsolidstate. These com- in palladacycles of azobenzene/bipyridine tions playanimportantroleinluminescence nificant Pd•••Pd interaction and such interac- NH(2), and as the closely related γL(g+)- γL(g+)- Ac-Val-Phe-NH in related I(a,g+) β-turn closely and γL(g-) the as and NH(2), carrageenates, formed by stoichiometric stability ofaseries dodecylammonium carrageenates dodecylammonium mesomorphic studyof andstructural Thermal a propensityforβ-turns,(Šarićetal.,2010). vation that peptides with bulky side chain have β-strand in contrast to the experimental obser- minimum energy structurecorrespondsto the βL(a)-βL(a) (blue)andβL(g-)-βL(a)(magenta). tures aresuperimposed:βL-βL(a)of Ac-Gly-Phe-NH Fig 4.FlexibilityoftheN-terminusinβL-structures.Fourstruc and Ac-Ala-Phe-NH A benchmarkstudyoftheconformational Structural characteristics andthermal 2 (red)andtwo Ac-Val-Phe-NH 2 , and Ac-Ala-Phe- , 2 structures, 2 (green) 2 the the - 2 . et al.,2010). (Vinceković properties thermal determining moiety playsa major rolein carrageenans structure is the cationicsurfactant, while the carrageenate mines thedodecylammonium and themajorfactorthatdeter - backbone attached tothecarrageenan electrostatically chains dodecylammonium ally disordered consists ofconformation sublayer nonpolar chains anda contains layersofcarrageenan sublayer a polar structure, inwhich layered have a carrageenates Dodecylammonium pared to that of the parent carrageenans. com- complexes the in ordering increased X-raydiffractionspecies while showedan andcarrageenan the dodecylammonium and hydrogenbondinteractionsbetween electrostatic the confirmed analysis spectral charged biopolymers,wereinvestigated.IR do- surfactant, andoppositely chloride decylammonium cationic of complexation oxygen (red),sulphate(yellow),nitrogen (blue). annealing dynamicssimulation:carbon (gray),hydrogen(white), Fig 5.ModelofDDAκCinthelayered LCstateafterashort -

43 2010. Report Annual RBI ZFK Education 11. Development of mathematical methods for the description of molecular structure, dinam- Division members provided more than 30 ics and reactivity, Darko Babić

ZFK undergraduate and graduate courses at the 12. Amino-beta-lactams-synthrons for biologi- Universities of Zagreb, Split, Rijeka, Osijek cally interesting compounds, Ivan Habuš and Dubrovnik. Research, developmental and international projects Awards 1. Development of new algorithms for the anal- Nives Ivić received Annual Award of “The ysis of protein active sites, Z. Štefanić (Bilat- Society of University Teachers, Scholars and eral project with Slovenia) Other Scientists – Zagreb” for young scien- 2. Theoretical Study of Enzyme Reactions, S. tists and artists. Tomić (Bilateral project with Slovenia) Nives Ivić, Marija Luić and Aleksandar 3. Mass spectrometry -based proteomic analy- Višnjevac received the RBI Director’s Award sis: Characterisation of horseradish esteras- for scientific excellence in 2010. es and snake venom proteins, and mapping of Fusarium parasites, I. Leščić Ašler (Bilat- eral project with Austria) Projects 4. Synthesis, identificatrion and biological activity testing of novel beta lactam cholesterol Projects supported by the Ministry absorption inhibitors, I. Habuš (Bilateral pro- of Science, Education and Sport ject with Austria)

1. Molecular structure and dynamics of systems with paramagnetic particles, Boris Rakvin Selected invited lectures 2. Surfactants, processes in solutions and at interfaces, Nada Filipović-Vinceković 1. Ljubić I (2010) Study of mechanism and kinet- 3. Measurement and effect of atmospheric oxi- ics of reversible hydrogen storage in titanium dants, Leo Klasinc doped sodium aluminum hydride. «Nano and 4. Advanced studies on chemical reactivity, Alek- Surface Science Approaches to Production sandar Sabljić and Storage of Hydrogen», Noordwijkerhout, 5. Developing methods for modelling properties The Netherlands, November 14-19, 2010. of bioactive molecules and proteins, Nenad 2. Tomić S (2010) Human dipeptydyl peptidase Trinajstić III: Insights into ligands binding. «Fourth Hum- 6. Design, synthesis and properties of organic boldt Conference on Computational Chemis- lignads and their metal complexes, Manda try», Varna, Bulgaria, July 12-15, 2010. Ćurić 3. Bosanac S (2010) Dirac equation and its 7. Protein-ligand interactions at atomic level, mysteries. Florida State University, Tallahas- Marija Luić see, Florida, USA, February 11, 2010. 8. Spectroscopy, chemical properties and reactions of biologically active molecules, Branka Kovač Selected organized 9. Control of atomic and molecular dynamics by conferences shaped electromagnetic fields, Nađa Došlić 10. Computational study of bio-macromolecules 1. 12th BRIJUNI CONFERENCE – Sustainable RBI Annual Report 2010. RBI and development of new algorithms, Sanja Sources of Energy, Brijuni, Croatia, August Tomić 30 – September 3, 2010.

44 Selected publications solution, and DFT calculations of transients in comparison with sulfur analogues. Phys 1. Močibob M, Ivić N, Bilokapić S, Maier T, Luić Chem Chem Phys 12 (2010) 6750. M, Ban N, Weygand-Đurašević I. Homologs 9. Leščić Ašler I, Ivić N, Kovačić F, Schell S, ZFK of aminoacyl-tRNA synthetases acylate car- Knorr J, Krauss U, Wilhelm S, Kojić-Prodić rier proteins and provide a link between ribo- B, Jaeger K-E. Probing enzyme promiscu- somal and nonribosomal peptide synthesis. ity of SGNH hydrolases. ChemBioChem 11 Proc Nat Acad Sci USA 107 (2010) 14585. (2010) 2158. 2. Višnjevac A, Gout J, Ingert N, Bistri O, Rein- 10. Kazazić S, Zhang H-M, Schaub TM, Emmett aud O. First Zn(II) bowl-complexes modeling MR, Hendrickson CL, Blakney GT, Marshall the tris(histidine) metallo-site of enzymes. AG. Automated data reduction for hydrogen/ Org Letters 12 (2010) 2044. deuterium exchange experiments, enabled 3. Dokli I, Matanović I, Hameršak Z. Sulfur ylide by high-resolution Fourier transform ion cy- promoted synthesis of N-protected aziridines: clotron resonance mass spectrometry. J Am A combined experimental and computational Soc Mass Spectrometry 21 (2010) 550. approach. Chem Eur J 16 (2010) 11744. 11. Bertoša B, Aleksić M, Karminski-Zamola G, 4. Juribašić M, Čurić M, Molčanov K, Matković Tomić S. QSAR analysis of antitumor active Čalogović D, Babić D. Unusual azobenzene/ amides and quinolones from thiophene se- bipyridine palladacycles: Structural, dynami- ries. Int J Pharmaceutics 394 (2010) 106. cal, photophysical and theoretical studies. 12. Rožman M, Gaskell SJ. Non-covalent in- Dalton Trans 39 (2010) 8769. teractions of alkalimetal cations with singly 5. Ljubić I, Clary DC. Towards understanding a charged tryptic peptides. J Mass Spectrom- mechanism for reversible hydrogen storage: etry 45 (2010) 1409. theoretical study of transition metal catalysed 13. Vinceković M, Pustak A, Liu F, Ungar G, Tušek- dehydrogenation of sodium alanate. Phys Božić Lj, Bujan M, Šmit I, Filipović-Vinceković Chem Chem Phys 12 (2010) 4012. N. Thermal and structural study of mesomor- 6. Šarić A, Hrenar T, Mališ M, Došlić N. Quan- phic dodecylammonium carrageenates. J Col- tum mechanical study of secondary struc- loid Interface Sci 341 (2010) 117. ture formation in protected dipeptides. Phys 14. Rakvin B, Maltar-Strmečki N, Kattnig D, Chem Chem Phys 12 (2010) 4678. Grampp G. ENDOR study on the dynamic 7. Molčanov K, Kojić-Prodić B. Salts and co- properties of the first stable paramagnetic crystals of chloranilic acid with organic bas- center in gamma-irradiated L-alanine crys- es: is it possible to predict a proton transfer tals. J Phys Chem A 114 (2010) 7500. in a crystal? CrystEngComm 12 (2010) 925. 15. Gredičak M, Matanović I, Zimmermann B, 8. Tobien T, Bonifačić M, Naumov S, Asmus K-D. Jerić I. Bergman cyclization of acyclic amino Time-resolved study on the reactions of organ- acid derived enediynes leads to the forma-

ic selenides with hydroxyl and oxide radicals, tion of 2,3-dihydrobenzo[f]isoindoles. J Org RBI Annual Report 2010. hydrated electrons, and H-atoms in aqueous Chem 75 (2010) 6219.

45 RBI Annual Report 2010. RBI

46 OVERVIEW OFTHE DIVISION in the fields of organic and bioorganic chem- andbioorganic in thefieldsoforganic research focus wasdirectedtowards basic principal The research. scientific in cellence ex- established their maintain to continued following laboratories: Biochemistry (ZOKB)consistsofthe and Chemistry Organic of Division The Head: KataMajerski DIVISIONAL ORGANIZATION and Biochemistry Division ofOrganicChemistry In 2010, the members of the Division a a a a a a a a a

nucleoside chemistry, Mladen Žinić Laboratory for supramolecular and chemistry, KataMajerski forsyntheticorganic Laboratory Zdenko Hameršak catalysis andbiocatalysis, forstereoselective Laboratory Lidija Varga-Defterdarović tide andglycopeptidechemistry, Laboratory forcarbohydrate,pep chemistry, David Smith chemistry, David Group forquantumorganic Piantanida Ivo interactions ofbiomacromolecules, Laboratory forstudyof spectroscopy, GoranBaranović formolecular Laboratory chemistry, DavorMargetić organic forphysical Laboratory biochemistry, Marija Abramić Laboratory forcellular

members ofseveraleditorial boards. cieties andbodies served as editors or so- and international active innational also separations. chiral The Division’s staff was cer compounds and newsystems for HPLC of new antican cuses arethedevelopment have beenestablished. Their respectivefo- spin off companies,BioZyneandChirallica, two researchers, OKB of discoveries the on and 7Ph.Dtheses.Based sion of5Diploma as thesupervi levels, aswell post-graduate and numerous coursesat the undergraduate in by participating education tions tohigher contribu significant provided Division the of was approved.Members patent application istry. tofundamentalresearch,1 In addition chem- organic quantum and allopeptidases and catalyticmechanismof relationship met- protein biochemistry, computational the structure-function and experimental troscopy; spec - molecular tides andpeptidomimetics, cules with DNA/RNA, the chemistryof pep- of smallmole- the interactions interactions, ular chemistry,gels and host-guest including istry, synthesis, supramolec stereoselective chem- such assyntheticandphysicalorganic areas in were made contributions important Amongst abroadrangeof research topics, chemical journals. primarily inhigh-ranking published, were contributions 60 Over istry. http://www.irb.hr/en/zokb - - - -

47 2010. Report Annual RBI ZOKB 48 RBI Annual Report 2010. ZOKB Fig. 2.Greensyntheticmethods self-assemebly intogelfibers. between oxalamideunits. The latterinducestheirunidirectional organo-gelators duetostrongintermolecularhydrogenbonding Fig. 1.Chiralbis(aminoacid)oxalamidesareversatilehydro-and were employed.(Štrukiletal.,2010). with zincandcadmiumchloride guanidines of cyano- reactions pose, mechanochemical was successfullydeveloped.For this pur- compounds the synthesisofmetal-organic compounds. metal-organic of inthemechanosynthesis reactivity dimensionalityand laboratory: Towards anenvironmentally-friendly assembly motifs(Frkanecetal.,2010). and self- properties on gelation reochemistry ste- gelator of influence the revealed which described, togetherwithnumerousstudies ChemistryattheRBI, was and Nucleoside lators inthe Laboratory of Supramolecular ge- oxalamide chiral of development the cle, effects. motifs andchirality self-assembly Gelation properties, TOP ACHIEVEMENTS An environmentallyfriendlymethodfor Arti- Feature invited an of context the In An unusualcisselectivity derivatives (Hernandez-Folgado et al., 2010). derivatives (Hernandez-Folgadoetal.,2010). advances in guanidiniocarbonyl-pyrrole-aryl results wereobtainedinthearticledescribing tions. Among which the mostcomprehensive molecules resulted in 7 new scientific publication of DNA/RNA with active small organic forms variousDNA/RNA of differentiation for simultaneous sensors molecule-multipurpose single Advances inthedevelopment of al investigations(Doklietal.,2010). dines and was explained using computation in theformationoftert-butylsubstitutedaziri- unusual salt. An sulfonium benzyl oxathiane a using imines, of bothisomersstartingfromN-protected good yield and with high enantiomeric excess to in moderate wereprepared oNs aziridines Fig. 3SulfurylidepromotedsynthesisofN-protectedaziridines methods DNA andds-RNA bytwoindependent,sensitivespectroscopic Fig. 4 A singlemolecule efficiently differentiates betweends- A rangeofN-Ts, Further progressinstudiesoftheinterac- cis selectivity wasobserved N-SES, N-Boc and- - chemical accuracy.chemical to testing Inaddition brid QM/MM method capableof producing hy- a at arrive we context, this in treatment QM a sophisticated tems. Byintroducing sys- treatment ofbiological computational has becomethe method of choice for the techniques (MM) molecular-mechanical and systems MM) methodfor useinbiological computationalAn accurate (QM/ enkephalin (Gredičaketal.,2010). to Met- activity compared antitumor nounced pro- showed synthesized that were pounds analogs. enkephalin-like The selectedcom- of a virtuallibrary by screening evaluated was peptides of small ability proliferative chines QSAR model to predict the anti- enkephalin-likestudy of analogs Computational structure-activity and gelationversatility(Čaplaretal.,2010). efficiency gelation different their in reflected gelling propertiesbetweenpositionalisomers The resultsrevealed dramatic differences in properties of gels was studied for the first time. gelators ontheirself-assemblymotifsand andSolvationGelators, Effects Racemic versus Enantiomeric Study, Structure–Gelation Gelators: Positionally IsomericOrganic isomeric xylenes. dramatically different gelationefficiencytowardverysimilar Fig. 5.Structuresofpositionallyisomericgelatorsand The mixing of quantum-mechanical (QM) quantum-mechanical of mixing The Ma- Vector Support a of capability The of isomerism positional of influence The with electron withdrawing cyano groupspro- with electronwithdrawing hyperacid andthefirst superacids new Computational designof longed theirlifetimes(Basarićetal.,2010). pro- significantly and 3-fold to up by mation increased for- QM associated the of yield quantum the moiety o-hydroxymethylphenol substituent intothe of theadamantyl duction chemical reactivitywasinvestigated.Intro- moiety, were synthesizedandtheirphoto- 2-hydroxy-2-adamantyl the with substituted Methides Lived Adamantylidene-Quinone Long- Photochemical Formation of (Čondić-Jurkić etal.,2010). enzyme, Pyruvate Formate-Lyasebacterial of the aspects ofthesubstratemechanism the method,wewereableto address key mechanical (QM)andhybridQM/MMtechniques. Fig. 6.Comparisonofenergyprofilesobtainedusingquantum adamantylidene quinonemethides. Fig. 7.Photochemicalformationandchemistryoflong-lived Polycyclic organic compounds substituted Polycyclic organiccompounds strategically Hydroxymethylphenols,

49 2010. Report Annual RBI ZOKB 50 RBI Annual Report 2010. ZOKB gas-phase deprotonation enthalpy of ΔH of enthalpy deprotonation gas-phase a having hyperacid true first the with nating culmi- molecules, acidic exceptionally vide eaoim n oiaie tes response. stress oxidative and metabolism in protein enzymes involved zinc-dependent metallopeptidase theM49 thiol sensitivityof Molecular basisfor the (Gredičak, etal.2010). pathway cyclization-elimination induced a novel,thermally data revealed tational compu- and Experimental cyclization. man to theBerg- relevant conditions them under and tested amino acidderivedenediynes Enediyine-peptide conjugates (Vianello etal.,2010). quasi-[14]annulene aromatic quasi-[12] and annulene antiaromatic represent pounds Two of the investigatedunsubstitutedcom- solution. dimethylsulfoxide in –26.7 of value mol kcal 242.8 = enediynes. Fig. 9.Cyclization-eliminationpathwayofamino-acidderived compounds ofunprecedentedstrength. Fig. 8.Polycyclicorganicmoleculesrepresentinghighlyacidic Metallopeptidases of theM49family are Metallopeptidases Wea seriesofacyclic havesynthesized –1 and a corresponding pK andacorresponding acid a

New equipment (Jajčanin-Jozić etal.,2010). compounds inactivation byorganomercurial for thefast residue, Cys639,isresponsible that onlyonecysteine The resultsrevealed cerevisiae Saccharomyces peptidaseIII), dase (dipeptidyl from theyeast Ser singleproteinvariantsoftheM49pepti- to Cys five all of mutationals the of analysis an to sulfhydrylreagents, susceptibility sual To elucidate the molecular basisof their unu- (Germany) wereinstalledduring2010. (Varian,USA), and a Mixer mill Retsch MM400 detector fluorescent and array diode with tem Fig. 11. MixermillRetsch MM400 tidase fromyeast Fig. 10.MolecularbasisforthethiolsensitivityofM49metallopep An HPLCgradientsemi-preparativesys- , wasperformed. - AWARDS Education tute ofChemistryinLjubljana, Slovenia. for an18-monthstayat theNationalInsti- Call FP7–PEOPLE–2009–IEF the under opment fromtheEuropeanCommission Marie CurieFellowshipforCareerDevel- 2010). (2008- Complications” Diabetic in Damage ship Award, “NovelPathways of Glycation Research FoundationPostdoctoralFellow Postdoctoral fellowships /awards plied QuantumMechanicsinParis,France. Scientist Prize 2010 of the Centre for the Ap- ences andtheinterdisciplinaryfieldofart. nical, biotechnical,humanisticandsocialsci- enviable achievements for in the fields of the natural, tech- Scholars Other and Teachers (2010) ofthe The Association ofUniversity securing ahighlyfundedscientificproject. Vianello received the RBI Director’s Award for lence in2010. excel- scientific for Award Director’s RBI the David M Smith and Vjekoslav Štrukil received Program attheUniversityofRijeka. of Zagreb;andtheMedicinalChemistry–PhD gram attheFacultyofScienceandUniversity grams, inparticular, theChemistry–PhDpro- pro- PhD several in involved are DOCB the of Members Dubrovnik. and Split Osijek, Rijeka, doctoral levelsattheUniversitiesofZagreb, numerous courses at the undergraduate and contributions tohighereducationbyproviding oet inlo eevd h Personal the received Vianello Robert Diabetes Ina NemetreceivedtheJuvenile Promising the received Vianello Robert Irena Doklireceivedthe Annual award Robert and Smith M David Basarić, Nikola Maksić, Mirjana Jerić, Ivanka Dokli, Irena Members of the Division provided significant - 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. and Sport Science,Education of the Ministry by Research supported Projects 2. 1. and Sport Science,Education of the Ministry by supported Research programs PROJECTS and function,DavidSmith. studies ofproteinstructure Computational istry andbiochemistry, ZvonimirMaksić. Brönsted and Lewis acids and bases in chem- teins, IvoPiantanida of small moleculeswithDNA, RNA and pro- synthesis andstudyofinteractions Design, Baranović spectroscopies, Goran tions andmolecular structures insolu- ligands, Macrocyclic Mirjana states, Maksić excited electronically and ground in processes bioorganic and Organic tems, DavorMargetić ‘Host-guest’sys- in polycyclic interactions tions ofhydrolases,Marija Abramić and proteininterac- Molecular enzymology pounds; LidijaVarga-Defterdarović com- of natural transformations Chemical obase and nucleotide derivatives, Biserka Žinić Synthesis ofnovelbiologicallyactivenucle- tional hybridmaterials,MladenŽinić of func- synthesis and in gels Self-assembly lecular Architecture, KataMajerski Blocks for Mo- Cage Compounds:Building and functionalresearch,VladimirVinković Chiral organicmaterials-synthetic,structural Hameršak molecules. Synthesisandreactivity, Zdenko active blocks forbiological Chiral building jana Maksić ganic molecularsystems, No: 0982933,Mir- of (bio)or reactivity and synthesis Design, No: 0982914,IvoPiantanida ecules of tumor and inflammatory processes, targeting macromol New smallmolecules - -

51 2010. Report Annual RBI ZOKB Research, developmental and SELECTED INVITED LECTURES international projects 1. Smith, David M. (Plenary) Studying Enzy- 1. Reinforcement of the Centre for Compu- matic Mechanism with QM/MM Techniques: tational Solutions in the Life Sciences, (6th An Application to Glycerol Dehydration «The ZOKB Framework Programme, EU–FP6–043749– 24th Molecular Modelling Workshop» Erlan- ReCompSoLS), 2007-2010. David M. Smith. gen, Germany, February 15 – 16, 2010. 2. Biocatalytic application of halohydrin de- 2. Smith, David M. A QM/MM Investigation of En- halogenases for production of chiral building zymatic Diol Dehydration. «From Solid State blocks, (UKF 3A Grant Agreement), 2009- To BioPhysics V - ‘From Physics To Life Sci- 2011. Maja Majerić Elenkov ences’» Cavtat, Croatia, June 12 – 19, 2010. 3. Computational prediction of structure and 3. Basarić, Nikola. Photochemistry of adaman- catalytic activity of new organic superacids, tyl-phthalimides: domino H-abstractions and (UKF 3A Grant Agreement), 2008-2010. decarboxylations. College of Chemistry and Robert Vianello Chemical Engineering Lanzhou University, 4. Photochemistry of polycyclic molecules: Lanzhou, P. R. China, June 21, 2010. From mechanistic studies to new drugs and 4. Mlinarić-Majerski, Kata. Synthesis and Ion medicinal applications (HRZZ) 2010-2013, Binding Abilities of Adamantane-annulat- Nikola Basarić ed Ionophores. College of Chemistry and 5. Mechanochemistry for the clean and efficient Chemical Engineering Lanzhou University, metal-catalysed synthesis of pharmaceuti- Lanzhou, P. R. China, June 21, 2010. cal targets and the study of their molecular 5. Smith, David M. Replica-Exchange Molecular recognition, Unity through knowledge fund, Dynamics Studies on Natural and Non-Natu- 2010-2012, Mirjana Maksić ral Opioid Peptides. «Australia-Croatia Work- 6. Artificial Receptors for Bacillus anthracis shop on Antimicrobial Peptides (AMP2010)» Specific Anthrose Detection. NATO Science MedILS, Split, Croatia, August 9 – 14, 2010. for Peace and Security Programme; CBP. 6. Vianello, Robert. One Group to Rule Them All EAP.SFP.983154. 2008-2011, Andreja Jakas - Polycyano Organic Compounds Are Record and Predrag Cudic Holding Superacids. «XVth International Work- 7. Fluorescent Anion Sensors: Synthesis and shop on Quantum Systems in Chemistry and Spectroscopic Characterization, Bilateral Physics (QSCP–XV)» University of Cambridge, project Croatia - P.R. China, 2009 - 2011, England, August 31 – September 5, 2010. Nikola Basarić 7. Smith, David M. Subtle Differences with Im- 8. Structure – function relationship in metallo- portant Consequences in Enzymatic Diol peptidase family M49, Bilateral project Croa- Dehydration «The 5th Central European tia – Austria, 2010-2011, Marija Abramić Conference – Chemistry towards Biology» 9. Design and synthesis of novel aryl--guan- Primošten, Croatia, September 8 – 11, 2010. idiniocarbonyl-pyrroles, and study of their 8. Basarić, Nikola. Photochemistry of adaman- interactions with DNA/RNA, Bilateral project tyl derivatives of hydroxybiphenyls: ESIPT vs. Croatia-Germany (DAAD ), 2009 – 2010, Ivo photosolvolysis „Pacifichem 2010 International Piantanida Chemical Congress of Pacific Basin Societies“, 10. Acid–Base and Tautomeric Properties of Bio- Honolulu, U.S.A. December 15 – 20, 2010. logically Relevant Compounds in Solution, 9. Smith, David M. On the mechanism of bio- Bilateral project Croatia - Slovenia, 2009 - logical glycerol dehydration using QM/MM 2010, Robert Vianello techniques. «Pacifichem 2010 International Chemical Congress of Pacific Basin Socie- RBI Annual Report 2010. RBI ties» Honolulu, Hawaii, USA, December 15 – 20, 2010.

52 SELECTED ORGANIZED Computational structure-activity study directs CONFERENCES synthesis of novel antitumor enkephalin analogs. Amino Acids 38 (2010) 1185. 1. 5th Central European Conference – Chem- 9. Čondić-Jurkić K, Zipse H, Smith D. M. A com- istry towards Biology, Primošten, Croatia, 8.- pound QM/MM procedure: Comparative per- 11.9.2010 (organizers (from DOCB) Marija formance on a Pyruvate Formate-Lyase model ZOKB Abramić, Zvonimir Maksić, Robert Vianello) system. J Comp Chem 31 (2010) 1024. 2. The 60th anniversary of RBI, Zagreb, 10. Basarić N, Žabčić I, Mlinarić-Majerski K, Wan 09.06.2010. (organizer Mirjana Maksić) P. Photochemical formation and chemistry of long-lived adamantylidene-quinone methides and 2-adamantyl cations. J Org Chem 75 SELECTED PUBLICATIONS (2010) 102. 11. Vianello R, Maksić Z. Polycyano derivatives 1. Sandala GM, Smith DM, Radom L. Modeling the of some organic tri- and hexacyclic molecules reactions catalyzed by coenzyme B12 depend- are powerful super- and hyperacids in the gas- ent enzymes. Acc Chem Res 43 (2010) 642. phase and DMSO - Computational study by 2. Kopriva I, Jerić I. Blind separation of analytes DFT approach. J Org Chem 75 (2010) 7670. in nuclear magnetic resonance spectroscopy 12. Gredičak M, Matanović I, Zimmermann B, Jerić and mass spectrometry: sparseness-based ro- I. Bergman cyclization of acyclic amino acid- bust multicomponent analysis. Anal Chem 82 derived enediynes leads to the formation of (2010) 1911. 2,3-dihydrobenzo[f]iso indoles. J Org Chem 75 3. Frkanec L, Žinić M. Chiral bis(amino acid)- and (2010) 6219. bis(amino alcohol)-oxalamide gelators. Gela- 13. Jajčanin-Jozić N, Deller S, Pavkov T, Macher- tion properties, self-assembly motifs and chiral- oux P, Abramić M. Identification of the reactive ity effects. Chem. Comm 46 (2010) 522. cysteine residues in yeast dipeptidyl peptidase 4. Štrukil V, Fábián L, Reid D. G., Duer M. J, Jack- III. Biochimie 92 (2010) 89. son G. J, Eckert-Maksić M, Friščić T. Towards 14. Eckert-Maksić M, Vazdar M, Ruckenbauer an environmentally-friendly laboratory: dimen- M, Barbatti M, Müller T, Lischka H. Matrix- sionality and reactivity in the mechanosynthe- controlled photofragmentation of formamide: sis of metal-organic compounds. Chem Comm Dynamics simulation in argon by nonadiabatic 46 (2010) 9191. QM/MM method. Phys Chem Chem Phys 12 5. Dokli I, Matanović I, Hameršak Z. Sulfur ylide (2010) 12719. promoted synthesis of N-protected aziridines: a 15. Margetić D, Ishikawa T, Kumamoto T. Excep- combined experimental and computational ap- tional superbasicity of bisguanidine proton proach. Chem Eur J 16 (2010) 11744. sponges imposed by the bissecododecahe- 6. Hernandez-Folgado L, Baretić D, Piantanida drane molecular scaffold: A computational I, Marjanović M, Kralj M, Rehm T, Schmuck C. study. Eur J Org Chem (2010) 6563. RBI Annual Report 2010. Guanidiniocarbonyl-pyrrole-aryl derivatives: 16. Alešković M, Basarić N, Mlinarić-Majerski K, structure tuning for spectrophotometric rec- Molčanov K, Kojić-Prodić B. Kesharwani M. K, ognition of specific DNA and RNA sequences Ganguly B. Anion recognition through hydro- and antiproliferative activity. Chem Eur J 16 gen bonding by adamantane-dipyrromethane (2010) 3036. receptors. Tetrahedron 66 (2010) 1689. 7. Čaplar V, Frkanec L, Šijaković Vujičić, N, Žinić 17. Patent – Maksić, Mirjana; Glasovac, Zoran. M. Positionally isomeric organic gelators: “N,N’,N’’-Tris-(3-dimethylaminopropyl)-guan- structure-gelation study, racemic versus enan- idine, the procedure of preparation from car- tiomeric gelators, and solvation effects. Chem bodiimide and application in reactions of Eur J 16 (2010) 3066. transesterification of oil”, European patent 8. Gredičak M, Supek F, Kralj M, Majer Z, Hol- EP 1786763, 2009 and Croatian patent HR losi M, Šmuc T, Mlinarić-Majerski K, Horvat Š. P20040341, 2010.

53 54 RBI Annual Report 2010. ZOKB OVERVIEW OFTHE DIVISION ing tradition in the investigation of ing traditionin the kinet- the investigation metal hydrides. has alongstand The division and compounds ganic polymers,intermetalic ceramics, zeolites,cluster compounds,or- glass- oxides, metal in are interests primary Our society. to transfer knowledge as well of new synthesis methodsas development and physicalpropertiesofvariousmaterials, re- high-quality microstructural search intothechemical, perform to is mission science. Our materials in excellence of centre tories: labora (ZKM) consistsofthefollowing of MaterialsChemistry The Division Head: SvetozarMusić DIVISIONAL ORGANISATION Division ofMaterialsChemistry The Division of Materials Chemistry isa of Materials The Division a a a a a

Rozelindra Čož-Rakovac Rozelindra materials, biological – forichtiopathology Laboratory Planinic Pavica complex compoundschemistry, Laboratory forsolidstateand and dosimetry, SavetaMiljanić forradiationchemistry Laboratory DamirKralj processes, forprecipitation Laboratory BorisSubotić materials, forsynthesisofnew Laboratory - - addition of copperoxide of addition modified by ceramics Zirconia TOP ACHIEVEMENTS activities are financially sponsored by the Ministry ofScience,EducationandSports. sponsored financially are activities research main Our recognition. international andreceived etry systemsweredeveloped dosim- chemical high-dose and Low-dose and itsapplications. radiation of ionizing effectsaspects ofthephysico-chemical all covers work its and Croatia in unit existing etry at laboratory the division istheonlysuch systems. chemistry anddosim- The radiation ics andmechanismsofdifferent precipitation phase composition and stability of ZrO of stability and composition phase ZrO poration ofCu Raman spectroscopy indicated that the incor- ments ofthepowderdiffraction patternswhile refine- Bail Le and Rietveld using determined position andaccuratelatticeparameterswere com- phase The clarified. was solution solid The effect of Cu 2 polymorph (Štefanić et al., 2010). polymorph(Štefanićetal., 2010). 2+ ions stabilize the tetragonal ionsstabilizethetetragonal 2+ ion incorporation on the ion incorporation on the http://www.irb.hr/en/zkm 2 -type -type

55 2010. Report Annual RBI ZKM 56 RBI Annual Report 2010. ZKM and [Cu(bpy)(C Ga la cmlxs [Cu(bpy)(C complexes, clear polynuclear species desired building blocks for thesynthesisof asconvenient3D architectures oxalate complexes with2Dand New mononuclear copper(II) temperature andtheMCE. used inof prediction the transition ordering theory andit is shownhow they couldbe with thedensityfunctional tices combined lat- magnetic of coupled model mean-field from 0 –5 T. changes are basedonthe Calculations field magnetic the under pounds, nrp cags ΔS changes entropy (MCE), intermsoftheisothermalmagnetic effectof themagnetocaloric the calculation intermetallic materials basedonGd-Ga storage inhydrogen effect magnetocaloric studieson Theoretical es ofmicrobialcells(Hrenovićetal.,2010). with thepredominantlyelectronegativesurfac- interaction their and complexes charged tively ing ofaluminiumandsiliconintheformposi- soluble water species originatingfromdissolutionandleach- of influence the by explained was A zeolite of influence toxic A onstrated. cerevisiae)microorganisamswasdem- ces junii) andunicellulareukaryotic(Saccharomy- produced zeolite A onprokaryotic(Acinobacter selected microorganisms Toxicity zeolites on commercial of eprtr cags ΔT changes temperature The isolated complexes have proved to be be to proved have complexes isolated The chitectures, respectively (Androš et al., 2010). actions, givingrisetothe overall 3Dand2Dar- gen-bonding (Fig.2.)and π 2,2’-bipyridine), aredominatedbythehydro- Crystal structuresofthenewmononu- Preliminary results werepresentedfor Preliminary 1-x The inhibitory influence of commercially commercially of influence inhibitory The Ge x , 0 < x > 1, and in Gd(Zn,Cu) com- Gd(Zn,Cu) in and 1, > x < 0 , 2 O 4 )(H mag 2 O)]·H and the adiabatic and ad – , inGdGa,Gd- π 2 2 O stackinginter- C 4 2 )(H O 4 (bpy = = (bpy 2 O)]·H 2 O spontaneously precipitated in system containing poly-L-aspartic acid. spontaneously precipitatedinsystem containingpoly-L-asparticacid. Fig. 2.Scanningelectronmicrographs ofthecalciumcarbonate Džakula etal,2010). (Njegić- properties physical its and solution cess of solid phase that is in equilibrium with pro- on theformation constraints modynamic cipal impotanceofkinetic,ratherthanther- polypeptides. The resultspointedto the prin- tion system of calcium carbonates/ synthetic in theprecipita composites wasinvestigated on theformationofbioinorganic romolecules and thepresenceofchargedsyntheticmac- materials Bioinorganic metal chemistry(Androšetal.2010). transition- oxalate-based the to contribution the above compounds representavaluable literature, the in limited very are complexes al fact that mononuclear oxalate–bipyridine met - heteropolynuclear species.Consideringthe convenient building blocks for new homo- and plane oftheunitcell[Cu(bpy)(C Fig. 1.Herring-bone-likehydrogen-bondingnetworkinthebc The role of metastable precursor phases The roleofmetastable 2 O 4 )(H 2 O)]·H 2 O - tors was investigated. The type and concen tion of the dopantsinLiF:Mg,Cu,P TL detec- detectors thermoluminescent of to characteristics dopants Influence of (Govorčin Bajsićetal.,2010). in thisconcentration region phase inversion dispersed → matrix a indicated TPU of wt% ofblendswith20and40 cal properties and mechani change inthemorphology thus affecting blendproperties. A stepwise process solidification the during matrix ene significantly growth inpolypropyl spherulite enhanced (PP) polypropylene to (TPU) blends polypropylene/polyurethane relationships of Structure-property concentrations. Fig. 4.GlowcurvesofLiF:Mg,Cu,P detectorswithvariousMg Fig. 3.Diferentialscanningcalorimeter, PyrisDiamondDSC The influence of the type and concentra and type the of influence The Addition of polyurethane thermoplastic - - - - says evaluated (Strunjak-Perović et al. 2010). as- genotoxic reptile for application potential mality tests (ENA) were described and their cleus (MN) and Erythrocyte nuclear abnor - Micronu- studies. genotoxicity in organisms model as time first the for used were reptiles gemonensis Hierophis snakein theBalkanwhip nuclear abnormalities erythrocytic themicronucleus and Evaluation of per (Kneževićetal.,2010). presence ofcertainactivators,namelycop- the important is more dependence energy shape for while sensitivity and curves glow of the the influence dopants the of tration Education New equipment uate andpost-graduate courses. conducting 16undergrad jek andDubrovnik Osi- Zagreb, of Universities the at programs to theeducational contributed the Division A, LeCroy, USA). ery oftherapeuticradiationdoses. deliv of verification and dose effective body dose, whole of organ for investigation nology D, CIRS Tissue& Phantom Simulation Tech- and Sports. tial supportofMinistryScience,Education The instrumentwaspurchasedbythepar- based systemsandorganiccompounds. polymer- complex polymers, of transitions phase especially microstructural properties, of thermaland amond DSCfordetermination As ectothermic analogues to fish models, models, fish to analogues ectothermic As In 2010,scientistsandresearchersfrom Centrifugal evaporator (WaveSurferoscilloscope Digital 64MXs- Number706- Pediatric Phantom,Model Diferential scanningcalorimeter, Pyris Di- - -

57 2010. Report Annual RBI ZKM AWARDS 3. Study of the processes of formation and growth of zeolite nuclei in the matrix of Srećko Kirin: Director’s Award for excep- amorphous aluminosilicate gel, Josip Bronić

ZKM tional scientific success, Ruđer Bošković In- (COGITO, Croatian-French Scientific Coop- stitute (2010), for obtaining a national or inter- eration Programme for 2009-2010). national project with more than 500.000 HRK 4. Physical-structural studies of mixed-halide (70.000 €). metal-atom clusters building blocks, Berislav Perić (COGITO, Croatian-French Scientific Cooperation Programme for 2009-2010). PROJECTS 5. Organometallic and inorganic bioconju- gates as potential enantioselective catalysts, Programs supported by the Ministry Srećko Kirin (UKF project, 2009/2010). of Science, Education and Sports 6. Free Radicals in Chemical Biology, Branka Mihaljević (European Cooperation in the field 1. New functional materials, Svetozar Musić of Scientific and Tecnical Research, COST CM0603, 2007-2011). Projects supported by the Ministry 7. Enhancing Quality Control Methods and Pro- of Science, Education and Sport cedures for Radiation Technology, Saveta Miljanić (IAEA Regional Technical Co-opera- 1. Synthesis and microstructure of metal oxides tion Project RER 8/017, 2010-2012). and oxide glasses, Svetozar Musić 8. Radiation protection dosimetry in medicine: 2. Study of influence of aluminosilicate precur- The risk of early and late health effects from sors on their transformation, Boris Subotić the use of radiation therapy, Saveta Miljanić 3. Precipitation mechanism of inorganic bio- (EURADOS Working Group 9, 2010- ) compatible and related materials, Damir Kralj 9. European Medical ALARA Network (EMAN), 4. Metal hydrides in clean energy systems, Željka Knežević (EURADOS Working Group Želimir Blažina 12 (2010- 5. Polynuclear metal systems – synthesis and 10. Radiation synthesis and characterization of properties, Pavica Planinić nanomaterials for health care, environmen- 6. Structure-property relationships of modified tal protection and clean energy applications, polymer materials, Ivan Šmit Irina Pucić (IAEA Regional Technical Co-op- 7. Physico-chemical effects of ionizing radiation eration Project, 2010-2012) in materials, Saveta Miljanić 11. Design of polymer blends and composites, 8. Subcellular biochemical and phylogenetic Irina Pucić (Croatian-Slovenian bilateral sci- diversity of aquatic organisms, Rozelindra entific cooperation, 2010-2011) Čoz-Rakovac 12. The effects of high-energy radiation on poly- meric systems, Irina Pucić (Croatian-Hun- Research, developmental and garian Intergovernmental S&T Cooperation, international projects 2010-2011). 13. Using Nuclear Techniques for the Char- 1. Metal oxides: structural and magnetic prop- acterisation and Preservation of Cultural erties, Mira Ristić (Croatian-Serbian Scien- Heritage Artefacts in the Europe Region, tific Cooperation Programme for 2009-2010). Branka Katušin-Ražem (IAEA RER/8/015, 2. Nanoparticle cell interaction: Molecular sign- 2009-2011). aling events induced by synthesized new na- 14. Characterization, intercomparasion and ap- nomaterials, Marijan Gotić (Croatian-German plication of radiophotoluminescence (RPL) RBI Annual Report 2010. RBI Scientific Cooperation Programme for 2010- dosimetry system according to international 2011). standards and protocols, Maria Ranoga-

58 jec-Komor (Scientific cooperation between 4. Babić-Ivančić, Vesna: Precipitation of min- Ruđer Bošković Institute and Chiyoda Tech- eral constituents of urinary stones in model nology Corporation, 2009-2010). systems chemical composition similar to the

15. Solid state detectors in mixed radiation fields urine, Vinča Institute of Nuclear Science, ZKM dosimetry, Saveta Miljanić (Croatian-Hun- Belgrade, Serbia, December 5, 2010. garian l Scientific and Technical Cooperation Programme for 2009-2010). 16. Optimization of the synthesis process of zeo- SELECTED ORGANIZED lite A with special emphasis on the control of CONFERENCES particulate properties (size and shape of zeolite A crystals), Boris Subotić (Bartex-Bartol 1. 3rd Croatian-Slovenian Symposium on Zeo- and Ruđer Bošković Institute cooperation lites, Trogir, 16.-17.9.2010. scientific research – 2nd phase). 17. Protection of oysters production from Mali Ston by verification of its autochthony, SELECTED PUBLICATIONS Ivančica Strunjak-Perović (Ministry of Agri- culture, Forestry and Water Management- 1. Krehula S, Musić S. Growth of uniform lath- VIP-project, 2008-2010). like Π-(Fe,Al)OOH and disc-like Π‑(Fe,Al)2O3 18. Breeding and selection in aquaculture: Re- nanoparticles in a highly alkaline medium. population of autochthonous brown trout in Mater Chem Phys 123 (2010) 67. the Gacka river, Natalija Topić Popović (Min- 2. Štefanić G, Musić S, Ivanda M. Effect of Cu2+ istry of Agriculture, Forestry and Water Man- ion incorporation on the phase development agement, 2008-2010). of ZrO2-type solid solutions during the thermal 19. Breeding and selection in aquaculture: Fish- treatment. J Alloys Comp 492 (2010) 536. ery 1961, Ivančica Strunjak-Perović (Ministry 3. Opačak I, Ristić M, Musić S. Preparation and of Agriculture, Forestry and Water Manage- characterization of hollow Π‑Fe2O3 irregular ment, 2008-2010). microspheres. Mater Lett 64 (2010) 2555. 4. Hrenović J, Željezić D, Kopjar N, Sarpola A, Bronić J, Sekovanić L: Antimicrobial activity SELECTED INVITED LECTURES of commercial zeolite A on Acinetobacter junii and Saccharomyces cerevisiae. J. Hazard- 1. Musić, Svetozar, 57Fe Mössbauer spectros- ous Mater 183 (2010) 655. copy in the investigation of the precipitation 5. Turković A, Dubček, P, Juraić, K, Drašner A, of iron oxides, 239th American Chemical So- Bernstorff S: SAXS Studies of TiO2 Nanopar- ciety (ACS) National Meeting , San Francis- ticles in Polymer Electrolytes and in Nano- co, USA, March 21-25, 2010. structured Films. Materials 3 (2010) 4979.

2. Miljanić, Saveta, Intercomparison of dosim- 6. Androš L, Jurić M, Planinić P, Žilić D, Rakvin RBI Annual Report 2010. eters for non-target organ dose measure- B, Molčanov K: New mononuclear oxalate ments in radiotherapy – Activity of EURA- complexes of copper(II) with 2D and 3D ar- DOS WG 9: Radiation protection in medicine, chitectures: Synthesis, crystal structures and The 6th International Workshop on Individual spectroscopic characterization. Polyhedron Monitoring of Ionizing Radiation, Oarai, Ja- 29 (2010) 1291. pan, November 29-30, 2010. 7. Njegić-Džakula B, Falini G, Brečević Lj, 3. Tartaro, Ivana; Mihaljević, Branka; Ferreri, Car- Skoko Ž, Kralj D: Effects of initial supersatu- la; Chatgilialoglu, Chryssostomos, Radiation- ration on spontaneous precipitation of cal- induced modification of lipids by thiyl radicals, cium carbonate in the presence of charged EUCHEM Conference on Organic Free Radi- poly-L-amino acids. J Colloid Interface Sci cals, Bologna, Italy, June 28- July 2, 2010. 343 (2010) 553.

59 60 RBI Annual Report 2010. ZKM 9. 8. S: Effect of dopants on TL characteristics of Miljanić M, Ranogajec-Komor Ž, Knežević 117. geenates. Carra- Dodecylammonium of Mesomorphic Study Structural and Thermal N: Vinceković Filipović- I, Šmit M, Bujan Lj, G, Tušek-Božić Ungar F, Liu A, Pustak M, Vinceković J Colloid Interface Sci J Colloid 341 (2010) 10. cology Ecotoxi- gemonensis. whip snakeHierophis and micronucleus in Balkan erythrocytic nuclearabnormalities of Evaluation Z: Tadić V, Benković M, Jadan N, Popović Topić R, Čož-Rakovac D, Lisičić I, Strunjak-Perović (2010) 573. LiF:Mg, Cu,P detectors. RadiatMeasur 19(2010)1460. 45 O include viruses, bacteria, yeast and other yeast and viruses, bacteria, include used inthesestudies ics. Modelorganisms ogy, and bioinformat genetics, biophotonics molecular biology, biochemistry, cell biol ology is based onthemethodsof modern verview oftheDivision consists ofthefollowinglaboratories: Biology (ZMB) The DivisionofMolecular Head: IgorWeber DIVISIONAL ORGANISATION Division ofMolecularBiology Research in the Division of molecular bi- of molecular in theDivision Research a a a a a a a a a a

Laboratory forGenotoxic Agents, Microscopy, HrvojeFulgosi forElectron Laboratory Genetics, ĐurđicaUgarković forEvolutionary Laboratory Plohl Miroslav Function ofHeterochromatin, forStructureand Laboratory Maja Osmak Laboratory ofMolecularandCell Biology, IvicaRubelj Laboratory for Molecular Genetics, Laboratory forMolecularGenetics, Lipa Čičin-Šain 2010), Branimir Jernej(untilMay Neurobiology,and Molecular ofNeurochemistry Laboratory Microbiology, DavorZahradka ofMolecular Laboratory Branka SalopekSondi ofChemicalBiology,Laboratory Dušica Vujaklija Microbiology, DavorZahradka forMolecular Laboratory - - technology. molecular bio- and biomedicine including biosciences, of field the in work professional of youngscientists for as thetraining as well of life, principles of theunderlying elucidation logical processesat the molecularleveland about bio- of ourknowledge eral broadening projectsisagen- of theseresearch purpose genomes. and of genes lution The primary cal processes in thecytoskeleton;andevo- dynami polyphenols; of activity antioxidant and structuralbiologyof plant hormones; physiology,of photosynthesis; biochemistry mechanisms regulatory neurotransmission; of mechanisms and regulatory background antibiotics; genetic ance tocytostaticsand resist- and agents toxic to responses lular growth, differentiationcel- and senescence; tion inmolecularregulationof cell division, (tran- information scription andtranslation);signaltransduc- genomic of expression DNAsequences; repetitive and organization and repair);genome cation, recombination regulation of genome variation(DNA repli study: maintenance of genome integrityand of fields following the comprise broadly sion lian cellsinculture. The projects inourDivi- luscs, insects, plants, rodents and mamma- fungi, cellularslimemoulds,worms,mol- http://www.irb.hr/en/zmb - -

61 2010. Report Annual RBI ZMB 62 RBI Annual Report 2010. ZMB frequently produce gross chromosomal rear- chromosomal gross produce frequently found thatrecAmutantsof D. radiodurans terium fidel - ity ofDNA repairinradiation-resistantbac - remarkable the impair that conditions Deinococcus radiodurans in DNA repair Fidelity of (Harcet etal.,2010). of freshwater spongesystematics evaluation raneus of classification taxonomic the of for revision the need markers, weemphasize with threegenetic analyses our phylogenetic karst species.Basedon and anendangered stygobitic sponge, area astheonlyknown raneus classification needed? molecular data -arevised from Spongillidae) inferred (Porifera, subterraneus Taxonomic Eunapius positionof (Harcet etal.,2010). sal andapicalmetazoangenomelineages dence foraslowrateofevolutioninbothba- phylogeny,in metazoan direct evi- providing than expected from their respective positions higher levels similarity show generally genes find- Our human and that sponge further indicate ings genomes. metazoan shaping in loss ofgene the importance emphasizing further of truetissues,therefore emergence the before even existed repertoire gene rich con- gene of tent andfunctionalrepertoire,thatthe terms in genomes complex have strikingly metazoan speciesalready thesimplestmetazoans of reveals acomplex genetictoolkit Demosponge ESTsequencing T op achievements We have described for the first time the time first the for described have We sponge The freshwater earliest the even that show results Our as well as the need for a thorough re- for athorough as theneed aswell karst Ogulin the in caves inhabits Deinococcus radiodurans . We have Eunapiussubter E. subter - -

carcinoma cells downregulation of carcinoma cells RhoB downregulation cells carcinoma human larygneal tocisplatin resistance in confers RhoBGTPase of Downregulation sistance (Brozovićetal.,2010). re- cDDP and formation species oxygen tive reac- cDDP-induced in protective role their and BCL-2 tionship betweenglutathione conditions. This reviewfocusesontherela- on thecelltypeandtreatment depends cell death for responsible pathway specific pathways. taneously activatedsignaling The death occursastheresultofseveralsimul- gen speciesthat can triggercelldeath.Cell oxy- reactive of formation the induces also recent datahavesuggestedthatcisplatin tocisplatinresistance glutathione andBCL-2, induced reactive oxygen species, relationship between cisplatin-The stability inD.radiodurans(Reparetal.,2010). DNAconsequently,of and, repair genome for fidelity the for essential is recombinase, rial results show thattheRecA protein,themainbacte- Our breaks. DNA radiation-induced rangements afterspontaneousorgamma- in CA3ST cells,as viewedundertheepifluorescencemicroscope. teins aftertransienttransfectionofthe appropriateplasmidvectors Fig. 1.CellularlocalizationofEGFP andEGFP-RhoBfusionpro- We havefoundthatinhumanlaryngeal of DNAApart fromtheinduction damage, two-hybrid screen,wedemonstrate recogni eukaryotic plantsandalgae. Usingayeast in chloroplasts – photosynthetic plastids–in found kinase histidine sensor bacterial-type function mechanism inplastid genome evolutionarily regulatory conserved photosynthesis genes:the Transcriptional of control cies (Mravinac&Plohl,2010). ples independentlyineachdescendantspe- speciation event, following the same princi- distributed in the genomes during or after the sponding ancestralfamilieswereformedand ing towhichrearrangedvariantsofthecorre- of thesametypesuggestascenarioaccord- ity of higher-order structures between families complex- the in and sequence nucleotide the tandem anddispersedfamilies.Differences in structural andorganizationalparallelismin the basicrepeatingunitsandalsorevealed of length and sequence nucleotide in larities ling speciesTribolium madensshowedsimi- Comparison withrepetitiveDNAsinthesib- constitute almost 60% of the whole genome. highly repetitiveDNA families,whichtogether beetle sibling species repetitive DNAs in Evolution of (Čimbora- drug Zovko etal.,2010). chemotherapeutic this to laryngeal carcinoma that acquired resistance in patientswith cisplatin treatment,especially of efficiency the improve might expression restoration of gene therapy involvingRhoB to Nevertheless, cisplatin. in cellresponse protective role does notprovideageneral we canassumethatRhoBdownregulation lines didnot alter theirsensitivity to cisplatin, tumor cell in additional since RhoBsilencing of thisdrug.However,accumulation cellular confers resistanceto cisplatin viadecreased Chloroplast Sensor Kinase (CSK) isa Sensor Kinase Chloroplast flour the in heterochromatin analyzing By Tribolium audax , wecharacterizedtwo - of concept hasbeenunclear.this elusive status testing, thescientific quantitative plicit ex - allow would that tools of lack the to due However,circles. biological beyond even os mirrors evolutionaryhistoryis well known transcriptome the of Phylogenetic age yaveetil etal.,2010) (Puthi- genes tory controlofphotosynthesis regula redox primary, this support to order in exist that RNAs, and proteins ribosomal genetic system such assome components, mechanism, whileplastidgenesalsoencode control transcriptional regulatory redox bust expression to be subject to this rapid and ro- retained inplastidsprimarilyorderfor their Co-located for Redox Regulation. Genes are and itsprimarygeneproductsare genome genomes: theplastid function of organellar for the hypothesis with theCoRR agreement isinprecise of photosyntheticeukaryotes this mechanismincytoplasmicorganelles Sigma factor-1(SIG-1). of The persistence RNAa bacterial-type (PTK), and Polymerase Sensor Kinase,Plastid TranscriptionKinase between: Chloroplast tion andinteractions tissue duringdevelopment. of thesignalingmoleculesidentifiedandquantifiedinreproductive nated inthedevelopingfruit.Planthormonesgibberellinsareone (Helleborus nigerL.)ishighlymediatedbyplanthormonesorigi Fig. 2.PostanthesisdevelopmentoftheChristmasroseflower The idea that the development of embry- that thedevelopment The idea - -

63 2010. Report Annual RBI ZMB 64 RBI Annual Report 2010. ZMB ments (Orešković&Klarica,2010). within thosecompart- tions thatpredominate condi on (patho)physiological sue depends between the entire CSF system tis- and surrounding exchange CSF The tissue. brain through thecapillarywallsintosurrounding of filtration and reabsorption of water volume consequence a as system CSF whole the in andabsorbed produced CSF ispermanently physiology. to thisnewhypothesis, According of CSF tion, andproposedanewhypothesis and absorp of CSFsecretion,circulation the classicalhypothesis have abandoned misinterpretations and interpretations of years ahundred fluid: nearly cerebrospinal of formation The Tautz, 2010). story in the journal Nature real. The results were is correlation that thephylogeny-ontogeny showed and across ontogeny genes pressed ex- of age phylogenetic the measures that approach new a devised (Germany) Plön in Biology Evolutionary of Institute Planck Max greb (Croatia)andDiethard Tautz, atthe Za- in Institute Bošković Ruđer the at Lošo geneticists, Evolutionary Tomislav Domazet- blood andISF-CSFthroughtheblood-brain barrier. the exchangeofwatersubstances(black arrows)betweenthe fluid (CSF),interstitial(ISF)and cerebralbloodvessels,and Fig. 3. A schemeoftheinterrelation betweenthecerebrospinal ae o or xeietl eut, we results, experimental our on Based published as acover published (Domazet-Lošo & - - in theelevated-plusmazetest. Fig. 4. Testing ofanxiety-likebehaviourWistar Zagreb-5HT rat versity Press(Čičin-Šain&Jernej,2010). Uni - Cambridge by published research”, er “Experimental models in serotonin transport- as a separate chapterinthebook included was model experimental our of description 5HTcentral andperipheral homeostasis. The impact ofaltered and behavioral biological the to address model an original rats provide mechanisms. As such, Wistar-Zagreb 5HT in brainserotonergic dysregulation showed of thismodel two sublines homeostasis 5HTin peripheral changes ures. Besides serotonin (5HT)meas- tremes ofperipheral ex- the for animals of breeding selective by transporter serotonin of /downregulation upregulation modelwithconstitutional rodent A 5HTrats: Wistar-Zagreb Milipore ScepterCellCounter Galaxy CO2 Incubator 170S (New Brunswick) Carl Zeiss„PrimoVert“ Microscope 30/70 TELSTAR Vertical Cabinet for cell and tissue culture CV- New equipment We developed a new genetic rat model Fig. 5.Celldifferentiation incocultureswithstromalcells. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. Research programs entiation, Mariastefania Antica Mariastefania entiation, interactions inlymphocytedifferMolecular - ance development,MajaOsmak resist- and agents cytotoxic to response Cell Lipa Čičin-Šain Serotonergic mechanismsinalcoholism, genome evolution,KrunoslavBrčić-Kostić in DNAThe roleofrecombination and repair and cellularaging,IvicaRubelj of immortalization mechanisms Molecular cell motilityandcytokinesis,IgorWeber dynamics in of thecytoskeleton Regulation and differentiation ofplastids,HrvojeFulgosi mechanisms ofphotosynthesis Regulatory and evolutions,HelenaĆetković structures, functions genomes: and Genes of eukaryoticgenome,ĐurđicaUgarković Evolution andfunctionof fast evolving portion myces biology, DušicaVujaklija studies ofStrepto- Fundamental molecular tion andrepair, DavorZahradka Molecular mechanismsof DNA recombina Orešković fluid, cerebrospinal of Hydrodynamics Branka Salopek-Sondi development, of plant regulation Molecular and behavior, BranimirJernej genes, proteins transmission: Serotonergic Ambriović Ristov effi- transduction cacy andresistanceto cytostatics, Andreja adenovirus of Increase Darko - 18. 17. 16. 5. 4. 3. 2. 1. Some outstandingpublications 8. 7. 6. 9. organisms, SenkaDžidić effectGenetic studiesofBPC-157 onmicro - Plohl Miroslav sequences, DNA satellite of tions interac- and functional Evolution, properties Mary Sopta Transcriptionalin eukaryotes, regulation carcinoma cells. resistance to cisplatin inhumanlarygneal of RhoBGTPaseconfers M. Downregulation Osmak N, Mikac G, Fritz T,Čimbora-Zovko Mol BiolEvol genetic toolkitofthesimplest metazoans. ESTosponge complex a reveals sequencing Dem- K, Vlahoviček WEG, Müller M, D, Wiens Perina H, Ćetković M, Roller M, Harcet Mol BiolEvol species. DNAs insibling repetitive of highly in evolution Mravinac B,PlohlM. Parallelism Res Rev64(2010)241. Brain interpretations andmisinterpretations. of years ahundred Nearly fluid: ebrospinal Orešković D, Klarica M. The formation of cer- (2010) 815. patterns. Nature togenetic divergence on- mirrors index age transcriptome based T,Domazet-Lošo TautzD. Aphylogenetically man fibroblasts. Abrupt Telomere Shorteninginnormalhu- Ivanković A, I. M, SmithJR Rubelj H, Huzak M, Fulgosi Ćukušić N, Vidaček Škrobot ans. BMCBiology in metazo- the emergenceofmulticellularity tracking of cancer genessuggests a linkto Phylostratigraphic T,TautzDomazet-Lošo D. pair 9(2010)1151. Deinococcus radiodurans bility inDeinococcus sta- genome DNA and of repair fidelity sures Zahradka D,K.RecA proteinas- M, D, Radman S, Slade J, Cvjetan Repar Rev Toxicol and BCL-2, andresistanceto cisplatin. Critical glutathione species, oxygen reactive cisplatin-induced between The relationship M. Osmak A, Ambriović-Ristov A, Brozović 40(2010)347. 27 (2010)2747. 27 (2010)1857. Exp Gerontol Cancer Let 8(2010)66. 295(2010)182. 45 (2010) 235. . DNA Re- 468

65 2010. Report Annual RBI ZMB 10. Plohl M, Petrović V, Luchetti A, Ricci A, Selected invited lectures Šatović E, Passamonti M Mantovani B. Long-term conservation vs. high sequence 1. Vujaklija, Dušica; Bielen, Ana; Abramić,

ZMB divergence: the case of an extraordinarily old Marija; Pigac, Jasenka. The power of SGNH- satellite DNA in bivalve mollusks. Heredity lipases from Streptomyces, Central Europe (Edinburgh) 104 (2010) 543. Symposium on Industrial Microbiology and 11. Bendix L, Bendix Horn P, Birk Jensen U, Microbial Ecology: Power of Microbes in In- Rubelj I, Kolvraa S. The load of short telom- dustry and Environment, Malinska, Krk, Cro- eres, estimated by a new method, Universal atia, September 22-25, 2010. STELA, correlates with number of senescent 2. Vujaklija, Dušica. Old concepts - new in- cells. Aging Cell 8 (2010) 383. sights into bacterial phosphorylation. Sum- 12. Harcet, M, Bilandžija H, Bruvo Mađarić B, mer School in Applied Molecular Microbiol- Ćetković H. Taxonomic position of Eunapius ogy - “From Signals to Drug” IUC, Dubrovnik, subterraneus (Porifera, Spongillidae) inferred Croatia, August 21-29, 2010. from molecular data - a revised classification 3. Herak Bosnar, Maja; Perina, Dragutin; Bago, needed? Mol Phylogenetics Evol 54 (2010) Ružica; Mikoč, Andreja; Harcet, Matija; 1021. Deželjin, Martina; Ćetković, Helena. Char- 13. Piljac-Žegarac J, Valek-Žulj L, Stipčević T, acterization of ancestral-type Grope I Nm23 Martinez S. Electrochemical determination gene/protein - structure and function is con- of antioxidant capacity of fruit tea infusions. served from sponges to humans, «8th Inter- Food Chem 121 (2010) 820. national Congress of the NDP kinase/Nm23/ 14. Puthiyaveetil SI,Iskander M, Jeličić B, awd Family-From Basic Science to Clinical Tomašić A, Fulgosi H, Allen JF. Transcrip- Application«, Heidelberg, Germany, October tional control of photosynthesis genes: the 25-28, 2010. evolutionarily conserved regulatory mecha- 4. Lukić-Bilela, Lada; Pleše, Bruna; nism in plastid genome function. Genome Mulaomerović, Jasminko; Tulić, Una; Biol Evol 2 (2010) 888. Ćetković, Helena. Speleobiology and Biospe- 15. Ayele BT Magnus V, Mihaljević S, Prebeg leology: Science against Blunders, «Stručni T, Čož-Rakovac R, Ozga JA Reinecke DM, seminar o zaštiti špilja I podzemne faune« Mander LN, Kamiya Y, Yamaguchi S, Salo- Ogulin, Croatia, January 30-31, 2010. pek-Sondi B. Endogenous gibberellin profile 5. Bilandžija, Helena; Ćetković, Helena; Jalžić, during Christmas rose (Helleborus niger L.) Branko. Strictly protected species facing flower and fruit development. J Plant Growth extinction? Dinaric cave clam (Congeria Reg 29 (2010) 194. kusceri), «Stručni seminar o zaštiti špilja i podzemne faune« Ogulin, Croatia, January 30-31, 2010. Book chapter 6. Andreja Ambriović-Ristov: Role of integrin ΠvΠ3 in tumor drug resistance and adenovi- Čičin-Šain L, Jernej B. Wistar-Zagreb rus mediated gene delivery, 10th Congress of 5HT rats: A rodent model with constitutional the Croatian Society of Biochemistry and Mo- upregulation/downregulation of serotonin lecular Biology with international participation, transporter. Experimental Models in Seroto- Opatija, Croatia, September 15-18, 2010. nin Transporter Research, Kalueff, Allan V.; 7. Meštrović, Nevenka; Castagnone-Sereno, LaPorte, Justin L. (eds.). Cambridge: Cam- Philippe; Pavlek, Martina; Car, Ana; Plohl, bridge University Press (2010); 214-243. Miroslav. How satellite DNAs in the “library” are created? Society for Experimental Biol- RBI Annual Report 2010. RBI ogy Annual Main Meeting 2010, Prag, Czech Republic, June 30-July 03, 2010.

66 8. Plohl, Miroslav. Contrasting patterns in 2. Orešković, Darko. Annual award to RBI sci- genomic composition of satellite DNA re- entists 2010., for publishing scientific paper peats. Society for Experimental Biology An- in high impact factor journal in the field of mo-

nual Main Meeting 2010, Prag, Czech Re- lecular biology ZMB public, June 30-July 03, 2010. 3. Hrvoje Fulgosi, Ruđer Bošković Director’s 9. Fulgosi, Hrvoje; Jurić, Snježana; Tomašić General Prize Paić, Ana; Lepeduš, Hrvoje; Schleiff, Enrico. 4. Miroslav Plohl: Annual IRB award (2010) for Unexpected links connecting photosynthesis the publication of a paper with high impact with plant stress responses. 5th Slovenian factor symposium on plant biology, Ljubljana, Slo- 5. Brankica Mravinac: Annual IRB award (2010) venia, September 06-09, 2010. for the publication of a paper with high impact 10. Weber, Igor; Faix, Jan; Filić, Vedrana; factor Marinović, Maja. Dual role of a Rac GTPase in the regulation of cell motility, From Solid State To BioPhysics V, Cavtat, Croatia, June Organization of 12-19, 2010. conferences, congresses 11. Ugarković, Đurđica. Transcription of satellite and meetings (all) DNAs and genome regulation in insects. Epi- genetics Europe, Dublin, Ireland, September 1. Summer Schools in Applied Molecular Mi- 14-15, 2010. crobiology: “From Signals to Drug” IUC, Du- 12. Ugarković, Đurđica. Developmental and envi- brovnik, Croatia, August 21-29, 2010 ronmental regulation of heterochromatin tran- 2. 5th Central European Conference - Chemis- scription. COST Action TD0905“Epigenetics- try towards Biology, Primošten, Croatia, Sep- Bench to Bedside” Workshop, Brno, Czech tember 8-11, 2010 Republic, November 22-25, 2010 3. Regional Biophysics Conference, Primošten, Croatia, September 15-18, 2010

Awards and appointments (all)

1. Harcet, Matija; Ćetković, Helena: Ruđer Bošković Institute Award for the scientific publication in 2010. RBI Annual Report 2010.

67 RBI Annual Report 2010. RBI

68 tories: (ZMM) consists of the following labora of The Division MolecularMedicine Head: TatjanaMarotti divisional organization a a a a a a a a a a a a a a a Division ofMolecularMedicine Stojković Animal CoreFacility, Ranko Neven Žarković Laboratory forOxidative Stress, Oliver Vugrek Group for Translation Medicine, Hećimović Neuropharmacology, SilvaKatušić Laboratory ofMolecular Jasminka Pavelić Laboratory ofMolecularOncology, Šeler Neuropsychiatry, DoroteaMück- Laboratory ofMolecular Tatjana Marotti Laboratory forReactiveRadicals, Sonja Levanat Laboratory forHereditaryCancer, Marijeta Kralj Laboratory ofExperimental Therapy, Sanja Kapitanović Laboratory forPersonalMedicine, Mirko Hadžija and Transplantation,Endocrinology Laboratory ofMolecular Bacteriology, MagdalenaGrce Laboratory ofMolecularVirology and Gall-Trošelj Laboratory ofEpigenomics,Koraljka Oncology, JelkaGabrilovac Hematology, Immunologyand Laboratory ofExperimental Mario Cindrić Laboratory forSystemsBiomedicine, - OVERVIEW OFTHEDIVISION tance, fordiseaseprevention. impor- and, ofprimary dures andtherapies proce- diagnostic of disease,forimproving the molecularmechanisms understanding for methods and strategies new refining and core, dedicatedto establishing biomedical represents an important neurodegeneration), and (e.g. inneuropsychyatry with clinicians collaborations through collected samples Human Tumor clinical Bankandnumerous and experimental therapy. The DMM, with its mechanisms of disease etiopathogenesis (DNA/proteins), molecular macromolecules etiology throughdisturbedmethylationof has beendevotedto mechanisms of disease research and attention Particular models. cology), stress-relateddiseasesandtheir chiatry,neuropharma neurodegeneration, aspects ofcancer,(neuropsy neuroscience to themolecular topics havebeenoriented of itslaboratories. The majorityofresearch ering a broad spectrum reflected in the name with themajorareasofhumanmedicine,cov- The mission of DMM is closely correlated of DMMisclosely The mission http://www.irb.hr/en/zmm - -

69 2010. Report Annual RBI ZMM 70 RBI Annual Report 2010. ZMM ovarian cancer(Hofstetteretal.,2010) er for recurrence – free and overall survival in shown tobeanindependentprognosticmark- p53andp73 splicingof Alternative nostic marker (Cigrovski Berković et al., 2010). dicating thispolymorphismasapotentialprog- mors andhigherIL-2serumvalues,stronglyin- to gastroenteropancreaticneuroendocrinetu- associated with both increased susceptibility the IL-2 promoter (-330; G) was shown to be tumors neuroendocrine gastroenteropancreatic in IL-2 -330T/Gpolymorphism TOP ACHIEVEMENTS the transcriptionalstartsidesof p73 isoforms. architecture oftheNH2terminus the p73gene. Arrows illustrate C-terminally truncatedproteinswere identified.Fig.2b. Thegene 5–10. Besidesfull-lengthp53,various p53splicevariantsencoding Fig. 1. The structureofthehumanp53gene, comprisingexons A novel p53 splice variant p53δ has been been has p53δ variant splice p53 novel A A single nucleotide polymorphism (SNP) in tients (Košičeketal.,2010). elin inthe CSF of disease pa- Alzheimer’s sphingomy of level increased significantly a controls, revealing healthy and age-matched samples from diseasepatients Alzheimer’s on CSF study conducted apilot through oped devel been has (CSF) fluid cerebrospinal of MS) ESI-QTOF to coupled (nano-HPLC sis fluid cerebrospinal in for phospholipidanalysis method mass spectrometry A novel nano-HPL the cellsurface(Malnaretal.,2010). likely due to decreased expression of APP at protein (APP) by β-secretase (BACE1), most precursor amyloid-β the of processing genic was showntoleadenhancedamyloido transporter NPC1 of cholesterol dysfunction protein APP of amyloidogenic processing accumulation Cholesterol promotes tumor regression(Jaganjacetal.,2010). animals and,insome rats, induced bearing tumor- of survival the extended growth, tumor experimental reduced mice and rats in tumors to thesiteofsolid applied host and growth intumor granulocytes of role The late endosomes/lysosomes. Fig. 2.NPC1-deficiencycausesaccumulationoffreecholesterolin Aanaly methodforsensitiveandreliable The accumulation of cholesterol upon from ahealthy Granulocytes isolated C ESI-QTOF - - - - method. Fig. 3.PhospholipidprofilingintheCSFbynano-HPLC-MS AWARDS AWARDS Education New Equipment in thefieldofmolecular medicine: ent of an international project (Oliver Vugrek) and asrecipi scientists (MoranaJaganjac) tian State Award for Science 2010 for young Croa - the Zorić), Slade, Arijana Neda Pivac, in scientific research (Magdalena Grce, Nela excellence for Award, Director’s RBI Annual bership. cancer prevention. work oncervical gium, fortheoutstanding Cancer (ECCA), Brussels,Bel- Association 2010. Award givenby the European Cervical Award forScience2010youngscientists. separately. toral courses. are listed All thesecollegia anddoc- master’s ing invariousbachelor’s, and activelyparticipat teachers, organizing ucation atdifferent asprincipal universities, The following scinetists were awarded the scinetists wereawarded The following mem- EACRHonorary Levanat: Sonja of Wisdom Grce: Pearl Magdalena Award Jaganjac: Morana State The Croatian in ed- DMMs researchersareveryactive 2. 1. 26MX (MSES)35.000EUR XD-79LS- Image AnalyzerAlliance (MSES) 20.000EUR High-Pressure Homogenizator - - 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. Sport Science,Education and of Ministry bythe Research supported projects 2. 1. and Sport Science,Education of the Ministry by supported Research programs PROJECTS Pavelić system, Jasminka of theimmune molecules the Gene therapyoftumorsby modulating otea Muck-Šeler system, Dor- tonergic andcatecholaminergic of sero- proteomics and Pharmacogenomics oxidative and stress, TatjanaMarotti ageing in appearance mor tu- and monooxygenase P450 Cytochrome tions and therapeutic potential, Sonja Levanat Signal transduction in tumors:Hh-Gli interac- DNA-damage treatment,MarijetaKralj The role of different cell deathresponsesto Katušić Hećimović of pathogenesis disease,Silva Alzheimer’s in the action ofcholesterol The mechanism gastrointestinal tumors,SanjaKapitanović of and pharmacogenetics genetics Molecular carcinoma, MajaHerakBosnar cell squamos oral in genes nm23 of role The islets frommousestemcells,MirkoHadžija Langherhans like structures the Obtaining lesions, MagdalenaGrce Aberrant DNA methylation in HPV associated ka Gall Trošelj headandnecktumours,Koralj in malignant changes and immunomodulatory Epigenetic normal cells,JelkaGabrilovac Role ofmembranepeptidasesontumorand sis, MarioCindrić Proteomic prostatetumor biomarker analy Program leader:DoroteaMuck-Šeler disorders. of neuropsychiatric chophysiology proteomics andpsy- Pharmacogenomics, research. Programleader:KrešimirPavelić Integrative genomics and proteomics in cancer - -

71 2010. Report Annual RBI ZMM 14. Molecular basis and treatment of psychiatric 7. b) The Instrument for Pre-Acces- and stress related disorders, Nela Pivac sion Assistance - IPA 15. The role of p53/p73 network in soft tissue sarcoma, Neda Slade Creation of research related infrastructure for ZMM 16. Stress, GABA-A receptors and mechanisms Translational Medicine and Applied Genomics, of action of neurophychoactive drugs, Du- PI: Oliver Vugrek bravka Švob Štrac 17. S-Adenosylhomocysteine hydrolase (AHCY) c) FP7-REGPOT-2007-1 deficiency: Molecular mechanims of a new human disease, Oliver Vugrek INTEgrating and Strengthening GEnomic Re- 18. Lipids, free radicals and their second messen- search in South-Eastern Europe” FP7-REG- gers in integrative oncology, Neven Žarković POT-2007-1 (PI: Fran Borovečki), members of the Executive Committee: Nela Pivac, Dorotea Additional projects at the DMM: Muck-Šeler

1. Eye immunology, Iva Dekaris d) BICRO 2. Pharmacogenetics in pediatric oncology, Jasenka Stepan Giljević The synthesis of disulphoxy and threesulphoxy 3. Genetic basis in development of pituitary tu- analogs for identification of peptides and pro- mors, Živko Gnjidić teins by mass spectrometry, Mario Cindrić 4. Molecular basis of aseptic instability of hip total endoprotesis, Robert Kolundžić 5. New therapeutic modalities in treatment of SELECTED INVITED LECTURES malignant diseases, Marko Radačić 1. Grce M: HPV vaccines and protection against Research development and cervical cancer. European perspective on international projects HPV vaccination - Strategy and Implementa- tion, 13th Central European Vaccination Ad- a) Billateral Projects: visory Group Meeting, Budapest, Hungary, October 29-30, 2010 1. The analysis of intracellular localization 2. Herak Bosnar M: Characterization of ances- and dynamics of A and B subunits of NDPK tral-type Groupe I Nm23 gene/protein - struc- (Nm23-H1 and Nm23-H2), Maja Herak Bos- ture and function is conserved from sponges nar (Croatia-France) to humans, «8th International Congres of the 2. Alzheimer disease – the role of cholesterol on NDP kinase/Nm23/awd Family-From Basic processing and localization of APP protein fam- Science to Clinical Applicationname», Hei- ily, Silva Katušić Hećimović (Croatia-Germany) delberg, Germany, October 25-28, 2010. 3. Improvements of adhesive properties of bio- 3. Katušić Hećimović S: The link between lipids medical materials by plasma treatment, Mo- and Alzheimer’s disease: a view from Nie- rana Jaganjac (Croatia-Slovenia) mann Pick type C disease, JSW Life Sci- 4. Genetic factors as indicators of suicide, Nela ence company, Grambach, Austria, Novem- Pivac (Croatia-Slovenia) ber 18, 2010 5. Molecular dynamic S-adenozylhomocystein 4. Slade N, Zorić A, Horvat A: The effect of p53 hidrolasys (SAHH) and its role in gene ex- isoforms on p73 activity in tumor cells. «1st pression, Oliver Vugrek (Croatia-Israel) International p53 Isoforms Meeting», Lyon, 6. SAHH defficiency: Proteomic analysis of France, September 13-15, 2010. RBI Annual Report 2010. RBI new methylation disturbance in humans, Oli- 5. Grce M: Report on the 4th Annual Summit on ver Vugrek (Croatia-Germany) Cervical Cancer Prevention (the European

72 Parliament, Brussels, January 26-27, 2010), Welfare, the Croatian Parliament, Zagreb, meeting at the Croatian Parliament on behalf Croatia, January 28, 2010 (Grce M) of the 4th European Cervical Cancer Pre- vention Week, Zagreb, Croatia, January 28, ZMM 2010 SELECTED PUBLICATIONS 6. Muck-Šeler D, Mustapić M, Nenadić-Šviglin K, Babić A, Nedić G, Nikolac M, Pivac N: Is 1. de Sanjose S, ...; Grce M, ...; Bosch XF. on the polymorphism in the serotonin type 1B re- behalf of the Retrospective International ceptor gene associated with alcohol depend- Survey and HPV Time Trends Study Group: ence and aggressive behavior? 24th Danube Human papillomavirus genotype attribution Congress of Psychiatry & 12th Central Euro- in invasive cervical cancer: a retrospective pean Neuropsychopharmacological Sympo- cross-sectional worldwide study. Lancet On- sium, Zagreb, Croatia, May 5-8, 2010. col 11 (2010), 1048. 7. Pivac N, Kozarić-Kovačić D, Nenadić-Šviglin 2. Hofstetter G, Berger A, Fiegl H, Slade N, K, Nikolac M, Nedić G, Mustapić M, Grubišić Zorić A, Holzer B, Schuster E, Mobus VJ, Re- Ilić M, Muck-Šeler D: Insomnia and seroton- imer D, Daxenbichler G, Marth C, Zeimet AG, ergic mechanisms in psychiatric disorders. Concin N, Zeillinger R. Alternative splicing 24th Danube Congress of Psychiatry & 12th of p53 and p73: the novel p53 splice variant Central European Neuropsychopharmaco- p53d is an independent prognostic marker in logical Symposium Zagreb, Croatia, May 5-8, ovarian cancer. Oncogene 28 (2010), 1997. 2010 3. Hernandez-Folgado L, Baretić D, Piantanida 8. Slade N, Zorić A, Horvat A, Hofstetter G, I, Marjanović M, Kralj M, Rehm T, Schmuck Berger A, Concin N, Zeillinger R: The effect C. Guanidiniocarbonyl-pyrrole-aryl deriva- of p53 isoforms/splice variants on p73 activ- tives: structure tuning for spectrophotomet- ity. «10th Congress of the Croatian Society of ric recognition of specific DNA and RNA se- Biochemistry and Molecular Biology», Opati- quences and antiproliferative activity. Chem ja, Croatia, September 15-18, 2010. Eur J 16 (2010), 3036. 4. Malnar M, Košiček M, Mitterreiter S, Omerbašić D, Lichtenthaler SF, Goate A, SELECTED ORGANIZED Hećimović S. Niemann Pick type C cells CONFERENCES show cholesterol dependent decrease of 1. First congress of Croatian Association for APP expression at the cell surface and its in- Cancer research HDIR-1 with international creased processing through the β-secretase participation ‘’From Bench to clinic’’, Novem- pathway. Biochim Biophys ActaMol Basis Dis ber 11, 2010 (Levanat S) 1802 (2010), 682. 2. 5th Croatian Congress of Alzheimer’s dis- 5. Cigrovski Berković M, Jokić M, Marout J,

ease with international participation, Zadar, Radošević S, Zjačić-Rotkvić V, Kapitanović RBI Annual Report 2010. Croatia, September 22-25, 2010 (Pivac N S. IL-2 -330 T/G SNP and serum values – po- and Muck-Šeler D) tential new tumor marker in neuroendocrine 3. 6th Croatian Congress of Pharmacology with tumors of gastrointestinal tract and pancreas international participation, Opatija, Croatia, (GEP-NETs). J Mol Med 88 (2010), 423. September 15-18, 2010 (Pivac N and Muck- 6. Pavišić R, Dodig I, Horvatić A, Mijić L, Sedić Šeler D). M, Rajić Linarić M, Gruić Sovulj I, Preočanin 4. 2nd Croatian Congress of Side Effects of T, Bukvić Krajačić M, Cindrić M. Differences Psychopharmacs with international paricipa- between reversible (self-association) and ir- tion, Rovinj, Croatia March 11-14, 2010 (Pi- reversible aggregation of rHuG-CSF in car- vac N and Muck-Šeler D) . bohydrate and polyol formulations. Eur J 5. Meeting of the Committee on Health and Pharm Biopharm 76 (2010), 357.

73 7. Jaganjac M, Poljak-Blaži M, Kirac I, Borović Chapter 4. In: Posttraumatic stress disorder S, Schaur RJ, Žarković N. Granulocytes as (PTSD): causes, symptoms and treatment. effective anticancer agent in experimen- Egan SJ (ed), Nova Science Publ, New York, tal solid tumor models. Immunobiology 215 2010, pp 109-139. ZMM (2010), 1015. 4. Pivac N, Nedić G, Nikolac M, Nenadić-Šviglin 8. Gredičak M, Supek F, Kralj M, Majer Z, Hol- K, Kozarić-Kovačić D, Mustapić M, Kovačić losi M, Šmuc T, Mlinarić-Majerski K, Horvat Z, Grubišić Ilić M, Borovečki F, Hajnšek S, Š. Computational structure-activity study di- Muck-Šeler D. Sleep disturbances and sero- rects synthesis of novel antitumor enkephalin tonergic markers in psychiatric disorders. In: analogs. Amino Acids 38 (2010), 1185. Advances in Psychology Research. Vol 71. 9. Košiček M, Kirsch S, Bene R, Trkanjec Z, Columbus AM (ed). Nova Science Publ, New Titlić M, Bindila L,Peter-Katalinić J, Hećimović York, 2010, pp 1-85. S. Nano-HPLC-MS analysis of phospholipids 5. Muck-Šeler D, Mustapic M, Nedic G, Babic in cerebrospinal fluid of Alzheimer’s disease A, Mimica N, Kozaric Kovacic D, Nenadic patients–a pilot study. Anal Bioanal Chem Sviglin K, Stipcevic T, Presecki P, Nikolac M, 398 (2010), 2929. Borovecki F, Folnegovic Smalc V, Kovacic Z, 10. Pinto B, Caciagli F, Ricco E, Reali D, Šarić A, Pivac N. Genetic and biochemical markers of Balog T, Likić S, Scarpato R. Antiestrogenic serotonergic and catecholaminergic systems and antigenotoxic activity of bee pollen from in neuropsychiatric disorders. In: Advances Cystus incanus and Salix alba as evaluated in Genetic Research, Chapter I, Urbano KV by the yeast estrogen screen and the micro- (ed) Nova Science Publ, New York, 2010, pp nucleus assay in human lymphocytes. Eur J 1-68. Med Chem 45 (2010), 4122. 11. Fučić A, Stojković R, Miškov S, Želježić D, Marković D, Gjergja R, Katić J, Jazbec A, Review articles: Ivičević Bakulić T, Demarin V. Transplacen- tal genotoxicity of antiepileptic drugs: animal 1. Car D, Sabol M, Musani V, Ozretić P, Levanat model and pilot study on mother/newborn co- S. Epigenetic regulation of the Hedgehog-Gli hort. Reprod Toxicol 30 (2010), 613. signaling pathway in cancer. Period Biol 112 (2010), 419. 2. Catela Ivković T. Cyclooxygenase isoforms in Book chapters: tumorigenesis. Period Biol 112 (2010), 441. 3. Gall Trošelj K, Novak Kujundžić R. Epig- 1. Levanat S, Levačić Cvok M. Cellular and Mo- enomics – A Bird’s Eye Perspective on the lecular Biology of Prostate Cancer. In: Pros- Genome. Period Biol 112 (2010), 411. tate cancer. Šamija M (ed). Zagreb, Medicin- 4. Levanat S. Croatian Association for Cancer ska naklada, 2010, pp 51-64. Research. Period Biol 112 (2010), 487. 2. Pivac N, Nedić G, Nikolac M, Muck-Šeler D. 5. Matijević T, Pavelić J. Toll-like receptors: cost Genetics of suicidal behaviour. In: Coping or benefit for cancer? Curr Pharm Design 16 with posttraumatic stress disorder in return- (2010), 1081. ing troops. Wounds of war II. NATO Science 6. Martin-Kleiner I, Gall Trošelj K. Mannose through peace and security series E: human 6-phosphate/insulin-like growth factor 2 re- and societal dynamics. Vol 68. Wiederhold ceptor in carcinogenesis. Cancer Let 289 BK (ed) IOS Press Amsterdam, 2010, pp 31- (2010), 11. 55. 7. Ozretić P, Levačić Cvok M, Musani V, Sabol 3. Pivac N, Kozarić-Kovačić D, Nedić G, Niko- M, Car D, Levanat S. In silico analysis of po- RBI Annual Report 2010. RBI lac M, Mustapić M, Babić A, Grubišić-Ilić M, tential structural and functional significance Kovačić Z, Muck-Šeler D. Genetic markers in of human breast cancer gene BRCA2 se- suicidal and non-suicidal veterans with com- quence variants found in 5 ‘ untranslated re- bat-related posttraumatic stress disorder, gion. Period Biol 112 (2010), 469. 74 O our countryandtheworld. environmental management for the benefit of toward anoptimum is oriented the Division systems, processesand states.Researchin research ofenvironmental tal andapplied verview oftheDivision Environmental Research Division ofMarineand following laboratories: mental Research (ZIMO) consists of the The Divisionof Marine andEnviron Head: Tarzan Legović DIVISIONAL ORGANIZATION a a a a a a a a a a a a Division contributions include fundamen include contributions Division

organisms, Emin Teskeredžić Aquaculture andpathologyofaquatic Molecular ecotoxicology, Tvrtko Smital Marijana Erk Biological effects ofmetals, Radioecology, DelkoBarišić Marijan Ahel compounds, biogeochemistry oforganic Analytical chemistryand imaging, Vesna Svetličić Bioelectrochemistry andsurface geochemistry, GoranKniewald Inorganic environmental Colloid geochemistry, IvanSondi Milivoj Lovrić Physical chemistryoftraces, Zlatica Kozarac Aquatic physicalchemistry, Milivoj Kuzmić Satellite oceanography, modelling, Tarzan Legović Informatics andenvironmental - - data inorderto identify criticalpointsinthe experimental real and systematically simulated on tested were setups experimental as various as well determination, intensities determination in metalcomplexing parameters data treatment Significance of T sities inCroatiaandabroad. post-graduate coursesweregivenat univer scientists. Finally, and70 21 undergraduate of Division the mentorship under defended, Ph.D, oneM.ScandtwoB.Sc. theses were 9 schoolswerecoordinated, four graduate organized, were conferences 10 and held a numberofinvitedlectureswere addition, In Contents. Current by indexed journals in results were published in 56 scientific papers to nanotechnology.down ography Research from satelliteocean mental science,ranging a wide rangeof topics inmarineandenviron and outsideclients. These projectsspanned the MinistryofScience,SportandEducation more than50researchprojectscontractedby op achievements Differentpeak of voltammetric procedures During 2010, division scientists workedon 2010,division During http://www.irb.hr/en/zimo - - -

75 2010. Report Annual RBI ZIMO 76 RBI Annual Report 2010. ZIMO to stevioside concentration between 1 x 10 x 1 between concentration stevioside to proportional The netpeakcurrentislinearly electro-reduction of carboxyl or ester by groups. caused probably and quasireversible is vs response V The Ag/AgCl. -1.3 at appears stevioside of voltammogram square-wave of E-960. Amaximum is Codex Alimentarius purpose sweetenerandtheirnumberinthe zil. Steviaglycosidesareusedasageneral positae) a perennialshruboftheAsteraceae(Com- rebaudiana (Stevia stevia of leaves the from extracted are that sides leaves stevia stevioside inextracts of of theconcentration of Determination (Omanović etal.,2010). parameters complexation reliable provided is found to be the most appropriate, as it for subtraction of the backgroundcurrent voltammogram whole the fitting by mination deter- intensities Peak (ASV). voltammetry tional stability constant) by anodic stripping pa- and condi concentration rameters (ligand complexation copper of determination (Strmečki etal.,2010). organic matterandparticles intheoceans of transport the for significance great of is iron.This or cadmium copper, for exudates phytoplankton of to capacity complexing influence the and contribution their tigate complementary methods in orderto inves- and electrochemical by examined were rial and thio/aminogroupsinpolymericmate- es (SAS),reducedsulfurspecies(RSS) carbohydrates, surfaceactivesubstanc- copper, cadmiumandiron. relation to theircomplexation of exudates andcarbohydrates in phytoplankton Characterization of et al.,2010). mol/L10 x 3.5 and Stevioside is one of several steviol glyco- of severalsteviol is one Stevioside Transparent exopolymer particles (TEP), family native to Paraguay and Bra- and to Paraguay native family -5 mol/L (Komorsky-Lovrić Bertoni), which is Bertoni),which -6 -

the above measured chemical species the abovemeasuredchemical es controlandmaintainaccumulationof all of the lakebottomwhereregenerationprocess- at only present were conditions sulfidic and anoxic activity, photosynthetic high to Due eu- Lake. trophicated seawaterlake,Rogoznica and anoxic small, a in 2008 and 2004 ton werereportedfordifferent seasonsin matter,and particulateorganic phytoplank classes, totaldissolved (RSS), nutrients,lipid environment ontheAdriatic coast Rogoznica Lake auniqueanoxic matter in organic and particulate nutrients, lipidclasses, dissolved sulfurspecies, Iodine,reduced gill cytosol of Mediterranean mussels (Myti- cytosol ofMediterranean gill in theheat-treated of metalconcentrations low-level metal exposure. Therefore, the use otic factorslimitsitsuse asabiomarkerof abi- and biotic levels withseveral lothionein metal contamination low-levelfor theassessmentof Mussel gillsasanindicator tissue voltammetry (Ćosovićetal.,2010). through phasesensitivealternatingcurrent surface the electrode on molecules organic capacitance causedby the adsorption of measuring thechangeof the doublelayer by directly at amercuryelectrode studied amino acids,andtheirdifferent fractionswas products of sugars and dins, condensation electrolyte interface. Adsorption of melanoi rally presentorganicsolutesat the electrode- natu- of mixture complex a of characteristics of adsorption sibility ofdirectinvestigation electrode matter (melanoidins)at theHg andsyntheticorganic natural of studies Electrochemical adsorption et al.,2010;Penezić2010). Iodine species, reduced sulfur species reduced species, Iodine The observed association of gill metal- of gill The observedassociation offer techniques Electrochemical pos- the

(Žic - - material (N-POM)whichactsasa“presodium “catalyst. responds topresenceofnitrogencontaining polymericorganic costatum atdifferent depositiontimes(td).Recordedpeakcor Fig. 1.Chronopotentiogramsindiatom cultureSkeletonema (Squalius cephalus chub European field-collected of tissue tinal ic metal and proteinlevelsinthe gastrointes (Squalius cephalus) field-collected European chub tissueof in thegastrointestinal levelsCytosolic metalandprotein cies. (Legovićetal.,2010). tainable yield policy causes extinction of spe- sus- maximum a only, predator top vesting har- except cases, all almost in that shown and foodcascadeecosystemmodels,it is species extinction inecosystems yieldcauses Maximum sustainable weight, butnotlength(Bavčevićetal.,2010). head seabream(Sparusaurata)compensates sea bream gilthead of growth Compensatory et al.,2010). tamination incoastalmarineareas(Dragun for theassessmentoflow-levelmetalcon- galloprovincialis lus The study reports for the first time cytosol- prey-predator,Using simple food chain It isfoundthatcompensatorygrowthingilt- ), living inalowmetal- ) should beconsidered - - abuse for five types of illicit drugs, including drugs, illicit of types five for abuse the estimation of drug allowed months, which plant of the city of Zagreboveraperiod8 was performedinthewastewater treatment their metabolites and substances choactive epidemiology using sewage abuse illicitdrug Estimation of zine compounds(Udiković-Kolićetal.,2010). exposed to long-term contamination by s-tria- bacterial communities originating from soils and therepertoireofcatabolicgeneswithin specificity genetic the into insights new vided ganization ofcatabolicgenes. The studypro- characterised byanalysingdiversityandor- enriched fromagrochemicalfactorysoilwere soil factory agrochemical communities enriched from bacterial atrazine-degrading of Taxonomic andfunctionaldiversity (Ivanković etal.,2010). programs. biomonitoring in exposure Cd of biomarker specific a as MT mussel zebra of taminant aresupportingevidenceforusage rinated pesticide)asanorganicchemicalcon- (organochlo- pentachlorophenol to exposure well asanabsenceofMT inductionfollowing The strong induction potential of cadmium as the ecologicallyrelevantconcentrationrange. closely correlated to MT than copper within ulation, especially by cadmium, being more firmed as an biological response to metal stim - duction inDreissenapolymorphawascon- biosynthesis inzebra mussel metallothionein Inducibility of (Filipović, MarijićandRaspor, 2010). period. spawning the during higher nificantly as follows: Zn>Fe>Cu>Mn>Cd andaresig- decrease levels cytosol, metal heat-treated riversection. Incytosoland contaminated Apsy- studyofvarious comprehensive Atrazine-degrading bacterial communities in- (MT) metallothionein of specificity The

77 2010. Report Annual RBI ZIMO heroin, cocaine, marijuana, amphetamine tor NSA 181/KE, and (ii) 2x Teledyne RDI and ecstasy. The results indicated that drug Acoustic Doppler Current Profiler (ADCP). abuse patterns in transition countries might be different from those reported for Western

ZIMO Europe, in particular with respect to the com- paratively increased consumption of heroin. Enhanced consumption of stimulating drugs (cocaine and ectasy) was systematically detected during weekends (Terzić et al., 2010).

Kinetic Analysis of Isothermal Crystallization of Potassium Aluminosilicate Ceramics (Leucite and Kalsilite) from Amorphous Potassium Aluminosilicate Precursors Fig. 2. Semi permeable membrane device used in monitoring Kinetics of the isothermal crystallization of Sava river. tetragonal modification of leucite and hexag- onal modification of kalsilite from differently prepared precipitated amorphous alumino- Education silicate precursors were investigated at three different temperatures. Kinetic analyses of Division members teach at all universi- the transformation processes have shown ties in Croatia and some universities abroad. that the crystallization of leucite occurs the In addition, they help coodinate four Ph.D. same way as the crystallization of kalsilite schools: Biophysics with the University and follows a pseudo-zero rate kinetic equa- of Split, Environmental Management and tion (Buljan et al., 2010). Oceanography with the University of Zagreb, and Environmental Protection and Nature Mathematical Model for Kinetics Conservation with the University J.J. Stross- of Organic Particle Adhesion at an mayer in Osijek. Electrified Interface

A mathematical model for the kinetics of an adhesion event based on consecutive two- and three-step processes of the first order was developed and tested. The proposed methodology enables determination of kinetic parameters of individual components of the adhesion process and the differentiation between the two- and the three- step processes (Ružić et al., 2010).

New equipment Fig. 3. Certificate of Marine Pollution Bulletin Highly Cited Author Award 2005-2009. RBI Annual Report 2010. RBI During 2010, the following larger equipment was obtained: (i) Precipitation collec-

78 Awards 4. Metal-induced cellular changes in aquatic organisms, Biserka Raspor Tarzan Legović was appointed Secretary 5. Organic compounds as molecular markers General of the International Society for Ecolog- of anthropogenic impact on the environment,

ical Modelling, as well as becoming a member Marijan Ahel ZIMO of the Committee on Biology, Ministry of Sci- 6. Nanoparticles in biogeochemical processes ence, Education and Sports of Croatia. Irena in the environment, Ivan Sondi Ciglenečki-Jušić and Marta Plavšić received 7. Surface force on atomic scale applied in the RBI Director’s Award for scientific excel- marine science and nanotechnology, Vesna lence in 2010, and Dušica Ivanković, Jasenka Svetličić Pavičić, Marijana Erk, Vlatka Filipović Marijić, 8. Development of nanotechnology- based and Biserka Raspor received the Highly Cit- sensor for bio- molecules, Croatian Science ed Author Award from Elsevier (Figure 1). In Foundation, Vesna Svetličić June 2010, Elsevier announced its 50 top 9. Systematic study of the Adriatic Sea as a cited articles published from 2005 till 2009 in basis for sustainable development of the Re- Marine Pollution Bulletin. Among the top 50 is public Croatia, Croatian National Monitoring the article entitled “Evaluation of the Mytilus Programme of the Adriatic Sea. galloprovincialis Lam. digestive gland metal- 10. Mathematical modeling of circulation and lothionein as a biomarker in a long-term field satellite sensing of boundary processes. study: seasonal and spatial variability” (Ma- Kuzmić, Milivoj rine Pollution Bulletin, 50, 2005: 1303-1313). 11. Pathology of aquatic organisms in relation to The principal author is Dušica Ivanković and pollution and aquaculture, Emin Teskeredžić co-authors are Jasenka Pavičić, Marijana 12. Ecological modelling for sustainable man- Erk, Vlatka Filipović Marijić, Biserka Raspor. agement of resources, Tarzan Legović The results presented in this article are based 13. Radionuclides and trace elements in environ- on multi-annual monitoring of MTs and metal mental systems, Delko Barišić levels in native populations of mussels from 14. Electroactive films for ecologically accept- the central part of Eastern Adriatic coast. The able conversion and energy storage, Višnja study was performed within the scope of the Horvat- Radošević Croatian National Monitoring Programme 15. Elektroanalitical research of microcrystal and for the Adriatic Sea (Project “Adriatic”). Link traces in dissolved substances, Milivoj Lovrić to article: http://dx.doi.org/10.1016/j.mar- 16. Information systems on environmental qual- polbul.2005.04.039. Ivan Sondi received an ity and risk, Ivica Ružić award for the most cited article in J. Colloid 17. Ecotoxicological significance of ABC trans- Interface Sci in 2010. port proteins in aquatic organisms, Tvrtko Smital

18. Networked Economy, Zoran Skočir and RBI Annual Report 2010. PROJECTS Jadranka Pečar-Ilić

Projects supported by the Ministry Research, developmental and of Science, Education and Sports international projects

1. Interactions of trace metal species in an 1. Reference Laboratory for water testing lab- aquatic environment, Ivanka Pižeta oratories in Croatia, Ministry of Regional 2. Biogeochemistry of metals in sedimentary Development, Forestry and Water Man- systems and soils in Croatia, Goran Kniewald. agement, Water Management Directorate), 3. Nature of organic matter, interaction with traces Biserka Raspor, Zlatica Kozarac, Dubravka and surfaces in environment, ZlaticaKozarac Hršak, Božena Ćosović

79 2. Adhesion of liposomes at electrode, Croatia- ture on soil functional microbial biodiversity. Slovenia cooperation in science and technol- ECOFUN-MICROBIODIV (under SEE-ERA. ogy, Nadica Ivošević DeNardis NET PLUS Joint Call), Ines Petrić 3. Investigation of the pollution of Croatian and 12. Radiochemical methods for determination

ZIMO Slovenian Northern Adriatic coast by organo- of radionuclides in water samples”, Croatian tin compounds and toxic metals with different – Slovenian bilateral cooperation, Martina analytical techniques, Slovenian-Croatian Rožmarić Mačefat project, Nevenka Mikac 13. Nanoparticles in aqueous environment: elec- 4. Supramolecular organization of polysaccha- trochemical, nanogravimetric, STM and AFM ride network in marine gels, Croatia-Slovenia studies, Irena Ciglenečki-Jušić cooperation in science and technology, 2010- 14. Hidrogeological studies of the Kupa River and 2011, Vesna Svetličić its tributaries”, Croatian – Austrian bilateral co- 5. Integration of geo-chemical laboratory tech- operation, Stanislav Frančišković-Bilinski niques, in situ field measurement, and hyper- 15. European network on applications of atomic spectral air-borne remote sensing for environ- force microscopy to nanomedicine and life mental risk assessment, Hungarian-Croatian science. COST Action TD 1002 (2010-2014), bilateral cooperation, S. Frančišković-Bilinski Coordinator of WG 4: Environmental Na- 6. On line toxicity sensors based on modifica- notoxicology and Nanoparticles Member of tion of membrane organisation, NATO sci- Core Managment, Vesna Svetličić, Tea Mišić, ence for peace and security programme, B. Amela Hozić Gašparović and L.A. Nelson 16. Radiological monitoring in Croatia (Nuclear 7. Complex investigation of organic aerosols in Power Plant Krško), Željko Grahek rural, urban and marine environment, Bilat- 17. Determination of radioactivity in the Danube eral collaboration with Air Chemistry Group of River in 2010., Delko Barišić the Hungarian Academy of Sciences at Uni- 18. The program of systematic testing and moni- versity of Pannonia, Veszprém, Kozarac, Z. toring of radioactive substances in the sea and Kiss, G. – sessile indicator organisms (National In- 8. Study of biogeochemical sulfur cycling in stitute of Radiological and Nuclear Safety), water column of Rogoznica Lake (Croatia) by Delko Barišić combination of chromatographic and electro- 19. Monitoring of freshwater fishery: fishing area chemical techniques. Bilateral collaboration of the Rivers Kupa, Korana, Dobra and with Max-Planck-Institute for Marine Microbi- Mrežnica”, Ministry of Agriculture, Fishery ology, Bremen, Germany, I. Ciglenečki-Jušić and Rural Development, E. Teskeredžić and T. Ferdelman 9. Biogeochemical processes in anoxic environment: Characterization of metal sulfide nano- Selected invited lectures particles by electrochemical methods. Bilater- al collaboration with University of Paris, IPGP, 1. Ciglenečki-Jušić I, Rogoznica Lake (Croa- France, I. Ciglenečki-Jušić and E. Viollier tia), a unique anoxic seawater system on 10. Biogeochemistry of sulfur, carbon, nutrients the Adriatic coast, Xiamen University, China, and redox sensitive elements in hypoxic/an- April 7-18, 2010. oxic aquatic environments. Bilateral collabo- 2. Bura-Nakić E, Reduced sulfur and iron spe- ration with State Key Laboratory of Marine cies in anoxic environments: Pavin Lake a Environmental Science, Xiamen University, meromictic krater lake as a study site, Xia- China, I. Ciglenečki-Jušić and Minhan Dai men University, China, April 7-18, 2010. 11. Development and evaluation of innovative 3. Mikac N, Geochemistry of marine sediments RBI Annual Report 2010. RBI tools to estimate the ecotoxicological impact from the Eastern Adriatic Coast, Final Work- of low dose pesticide application in agricul- shop of the project “Marine science and

80 coastal management in the Adriatic, western SELECTED ORGANIZED Balkan. An education and research network“, CONFERENCES Cavtat, May 2010. 4. Ahel M, Relative contribution of polar con- 1. “Characterisation of hazardous chemical

taminants to the toxicological profiles of the contamination – from environmental chemis- ZIMO wastewater effluents of the city of Zagreb try and toxicology to risk assessment”, Du- (Croatia) // SETAC Europe Annual Meeting: brovnik, 23.-26. 09.2010 (M. Ahel, Chairman) Science and Technology for Environmental 2. 11th Reunion of the Laboratories authorised Protection, Seville, Spain, May 23-27, 2010. for water analysis, Biograd na moru, Croatia, 5. Ciglenečki-Jušić I, Distribution of redox-sen- November 16-19, 2010. Member of the Or- sitive trace elements in the water column and ganizing Committee: Biserka Raspor. sediments of seawater lake (Rogoznica lake, 3. Collaborative meeting. Charge density Croatia) an unique anoxic environment on waves: small scales and ultrashort time. Vu- the Adriatic coast, GEOTRACES Medditerra- kovar, Croatia, 28.10.–30.10. 2010. Vesna nean workshop in the frame of Cost Actions Svetličić (collaborator) ES0801 “The ocean chemistry of bioactive 4. IIIrd International Meeting on AFM in Life Sci- trace metals and paleoclimate proxies”;Nice, ences and Medicine. Red Island, Croatia, France, October 3-6, 2010. 12.05.-15.05.2010. Vesna Svetličić (chair of 6. Frka-Milosavljević S, Characterization of organizing committee), Amela Hozić Zimmer- surface active substances in the natural sea mann, Tea Mišić Radić, Galja Pletikapić (lo- surface microlayers of the coastal Middle cal organizing committee) Adriatic stations, GEOTRACES Medditerra- 5. Regional biophysics conference. nean workshop in the frame of Cost Actions Primošten, Croatia, 15.09.-18.09. 2010. ES0801 “The ocean chemistry of bioactive Vesna Svetličić (co-organizer and member of trace metals and paleoclimate proxies”; Nice, International Advisory Board) France, October 03-06, 2010. 6. Adriatic School on Nanoscience (ASON -1). 7. Bura-Nakić E, Voltammetric characterization From Atomic to Macroscopic Nanostructures. of iron sulphide species in model solutions Dubrovnik, Croatia, 19.09.–23.09.2010. Ves- and natural samples, Max Planck Institute for na Svetličić (programme Committee member) Marine Microbiology, Bremen, Germany, Oc- 7. International meeting on fishery: Prospect of tober 10-20, 2010. Croatian fishery. Fishery and health of fish. 8. Omanović D, Distribution of trace metals in Vukovar, Croatia, 14.4.-15.4.2010. Zlatica estuary and seawater, Université du Sud Teskeredžić, Emin Teskeredžić (programme Toulon Var, Garde, France. October 13 – De- Committee member) cember 11. 2010) 9. Ahel M, Prioritization of hazardous chemi-

cal contaminants in the Sava River basin Selected publications RBI Annual Report 2010. using EDA approach. „Characterization of hazardous chemical contamination-from en- 1. Omanović D, Garnier C, Louis Y, Lenoble vironmental chemistry and toxicology to risk V, Mounier S, Pižeta I. Significance of data assessmen”t, Inter-University Center, Du- treatment and experimental setup on the brovnik, October 23-26, 2010. determination of copper complexing param- 10. Klanjšček T, „From bacteria to whales: re- eters by anodic stripping voltammetry. Anal lating environment to growth and reproduc- Chim Acta 664 (2010) 136. tion using Dynamic Energy Budget Theory”. 2. Žic V, Carić-Glunčić M, Viollier E, Ciglenečki Global Center Of Excellence (G-COE) Fo- I. Intensive sampling of iodine and nutrient rum, Yokohama National University, Yoko- speciation in naturally eutrophicated anchial- hama, Japan. December 13-20, 2010. ine pond (Rogoznica Lake) during spring and

81 summer seasons. Estuar Coast Shelf S 87 in gastrointestinal cytosol of indigenous Eu- (2010) 265. ropean chub. Comp Biochem Phys C 152 3. Legović T, Klanjšček J, Geček S. Maximum (2010) 133. sustainable yield and species extinction in 11. Ivanković D, Pavičić J, Beatović V, Sauer-

ZIMO ecosystems. Ecol Model 221 (2010) 1569. born Klobučar R, Klobučar G, Igor V: Induc- 4. Bavčević L, Klanjšček T, Karamarko V, Aničić ibility of metallothionein biosynthesis in the I, Legović T. 2010 Compensatory growth in whole soft tissue of zebra mussels Dreissena gilthead sea bream (Sparus aurata) compen- polymorpha exposed to cadmium, copper sates weight, but not length. Aquaculture and pentachlorophenol. Environ Toxicol 25 301 (2010) 57. (2010) 198. 5. Strmečki S, Plavšić M, Steigenberger S, Pas- 12. Terzić S, Senta I, Ahel M. Illicit drugs in sow U. Characterization of phytoplankton ex- wastewater of the city of Zagreb (Croatia)- udates and carbohydrates in relation to their Estimation of drug abuse in a transition coun- complexation of copper, cadmium and iron. try. Environ Pollut 158 (2010) 2686. Mar Ecol - Prog Ser 408 (2010) 33. 13. Udiković-Kolić N, Hršak D, Devers M, Klepac- 6. Ćosović B, Vojvodić V, Bošković N, Plavšić Ceraj V, Petrić I, Martin-Laurent F. Taxonomic M, Lee C. Characterization of natural and and functional diversity of atrazine-degrading synthetic humic substances (melanoidins) by bacterial communities enriched from agrochemical composition and adsorption meas- chemical factory soil. J App Microbiol 109 urements. Org Geochem 41 (2010) 200. (2010) 355. 7. Penezić A, Gašparović B, Burić Z, Frka S. 14. Buljan I, Kosanović C, Subotić B, Novak T, Distribution of marine lipid classes in salty Ristić A, Gabrovšek R, Kaučič V, Mišić Radić Rogoznica Lake (Croatia). Estuar Coast T. Kinetic Analysis of Isothermal Crystalliza- Shelf S 86 (2010) 625. tion of Potassium Aluminosilicate Ceramics 8. Komorsky-Lovrić Š, Novak I, Novak B. Meas- (Leucite and Kalsilite) from Amorphous Po- urement of Stevioside by Square-wave Po- tassium Aluminosilicate Precursors. Cryst larography. Electroanal 22 (2010) 2211. Growth Des 10 (2010) 838. 9. Dragun Z, Erk M, Ivanković D, Žaja R, 15. Ružić I, Pečar-Ilić J, Ivošević DeNardis N. Filipović Marijić V, Raspor B: Assessment Mathematical model for kinetics of organic of low-level metal contamination using the particle adhesion at an electrified interface. J Mediterranean mussel gills as the indicator Electroanal Chem 642 (2010) 120. tissue. Environ Sci Pollut R 17 (2010) 977. 10. Filipović Marijić V, Raspor B: The impact of fish spawning on metal and protein levels RBI Annual Report 2010. RBI

82 O environment. projects regardingprotection ofthemarine purposes aswellforseveral international toring ofthe Adriatic Seafor government sic research, the Center is involved in moni- the northern Adriatic Sea. In addition to ba- in planktonic and benthic communities of subcellular to the regional scale, especially cesses inthemarineenvironmentfrom search isthemultidisciplinarystudyofpro- verview oftheDivision consists ofthefollowinglaboratories: The CentreforMarineResearch Head: NenadSmodlaka DIVISIONAL ORGANISATION Center forMarineResearch The missionoftheCenterforMarineRe- a a a a a a

Travizi systematics ofbenthos, Ana Davorin Medaković nanostructure andradioecology, toxicology, RenatoBatel formarinemolecular Laboratory Laboratory forecologyand marine ecosystem,RobertPrecali Laboratory forprocessesinthe Laboratory forbiomineralization ecotoxicology, NevenkaBihari Laboratory formarine ecology, MirjanaNajdek formarinemicrobial Laboratory vironmental stress(Hamer etal.,2010). be usedasatracerofen- might shells valve inlower that mussel shellcarbonates indicatingbi- reflected is influx freshwater that period intheeastern Adriatic coastshowed during a measured ature variations ten year temper- and Salinity 2010). al., et (Rončević centers industry chemical city wasteand tamination in shells collectednearharbours, by ICP-AES showed a certain levelof con- pollution environmental conditionsand of galloprovincialis asanindicator themusselMytilus Utility of al., 2010). et (Fafanđel activity DNase acid high tains digestion,con- some, thesiteofintracellular the endo geneity ofneutralDNases,while variability andhetero- high interindividual expresses environment, the with municates Tethya aurantium marinesponge and endosomefrom Deoxyribonucleases inthecortex T op achievements Metal content in mussels as measured Metal contentinmussels com- that section sponge a cortex, The http://www.irb.hr/en/cim -

83 2010. Report Annual RBI CIM 84 RBI Annual Report 2010. CIM enzymes (Ivančićetal., 2010b). was low and mainlydueto the activity of free matter were P limited. In deeper waters APA organic produced phytoplankton grading upper layers. In these months bacteria de- plankton APA, thoughnotbacterial APA, in phyto- waters reduced from deeper supply P extra an autumn In bacterial. over inated dom- activity usually biomass, phytoplankton organic taking intoaccount although, phosphorus dissolved exploited communities Adriatic Sea in thenorthern alkaline phosphatase activity(APA) Phytoplankton andbacterial (Ivančić etal.,2010a). and changeintheirgrowthcharacteristics sible factorsfortheobservedHP decrease respon most the be might changes quality and substrate supply reduced conditions, hydrographic that changed indications were after 2003. a substantialdecrease There (HP) showed trophic prokaryoteabundance Adriatic Sea characteristics inthenorthern andgrowth abundance prokaryote changes Long-term in heterotrophic mically (Hernandez-Becerriletal.,2010). to fourstrongspinesoftenbranchingdichoto- face smoothorwithsmallgranules,andone paired, both valves convex to domed and sur- show morphologicalvariability, fromsolitaryto resting spores commonly found, which also most characteristicfeatureis,however, the ously studiedbyelectronmicroscopy. Its Adri- northeastern atic Sea. This specieshadnotbeenprevi- the in diatoms frequent and abundant most the of one is vixvisibilis theAdriaticfrom Sea diatom vixvisibilis Chaetoceros the of andecology Morphology During latespringandsummermicrobial period hetero- During the 1990–2008 The chain-formingdiatomChaetoceros - family forscience(Mikacetal.,2010). Sundkvist, Mikac&Pleijel,2010,ofSyllidae new species,Erseiaoligochaeta sulted inthedescriptionofanewgenusand polychaetes foundinthe vicinity of Rovinj re- Adriatic Sea northern the Syllidaefrom New speciesof crete constructions. grazers differsand con- sandstone between of biodiversity local (Australia), Harbour ney seawalls limpetson intertidal Distribution of Fig. 1.Erseiaoligochaeta,newgenus,species. Morphological and molecular analysesof and molecular Morphological n riiil ufcs saal) inSyd- (seawalls) surfaces artificial On Patelloida latistrigata Nygren,

New equipment (Nygren etal.,2010). to of the establishment warm-water species due diversity of modifications with changes, long-term the areaispossiblyundergoing the northern Adriatic Sea and indicatethat for records are new which species of 13 ings chaeta) from the Rovinj area resulted in find- Adriatic Sea (Polychaeta) for thenorthern Syllidae of New records Adriatic (Mozetičetal.,2010). existence of oligotrophication in the Northern to the point and ammonia of phosphateand in concentrations decrease cently evidenced with there- mation. Resultsareconsistent infor- satellite-derived from year−1) m−3 mg trations is confirmed all over the basin (−0.11 cent substantialreductionof Chl aconcen re- River. The Po the of influence the under which ismoremarkedintheeutrophicarea ofinvestigation, a reductionintheperiod tendency towardsChl demonstrate aglobal in theNorthern 1970–2007 Adriatic Sea a (Chl a) chlorophyll in the collected period on observations field of base data checked a TimeSeries Adriatic: Evidence from Chlorophyll, theNorthern Oligotrophication of effect (Ivešaetal.,2010). algae, whereas S. denticulata had noteffectof P. onsurvival latistrigata ; denticulata and ofSiphonaria conspecifics of survival reducing densities competitor,the stronger was increased with Research on the family Syllidae (Poly- Research onthefamilySyllidae quality- and updated the of results The Cryostat reduced amountsof macro- P. latistrigata had nosuch S. denticulata - .

Fig. 2.Syracosphaerapulchra,phytoplanktonspecies. Education 7. 6. 5. 4. 3. 2. 1. and Sport Science,Education of the Ministry by supported Research programs ties ofZagreb,Osijek,Split,andDubrovnik. ate courseswereconductedattheUniversi and post-gradu scientists.Under- Center’s The majority of courses are organized by the ate) at the Juraj DobrilaUniversityinPula. of MarineSciencesStudies(undergradu The Center is involved in theorganisation The Centerisinvolved Smodlaka Nenad “Adriatic”), (Project Croatia” of public of theRe - development base forsustainable “Systematic researchofthe Adriatic Seaasa programme monitoring national Croatian Travizi Adriatic: naturalandhumanimpacts, Ana Biodiversity ofbenthiccommunitiesinthe northern Adriatic ecosystem,RobertPrecali the in changes of long-term Mechanisms Najdek munities innorthern Adriatic front, Mirjana com- of microbial Structure andphysiology ganisms, DavorinMedaković or- inmarine processes Biomineralization organisms, NevenkaBihari marine on contamination of effect Ecotoxic thesis inmarineinvertebrates,RenatoBatel biosyn onprogrammed Impact ofpollution - - - -

85 2010. Report Annual RBI CIM Some outstanding 8. Mikac B, Musco L. Faunal and biogeographic publications analysis of Syllidae (Polychaeta) from Rovinj (Croatia, northern Adriatic Sea). Sci Mar 74

CIM 1. Rončević S, Pitarević Svedružić L, Smetiško (2010), 353. J, Medaković D. ICP-AES Analysis of Metal Content in Shell of Mussel Mytilus galloprovincialis from Croatian Coastal Waters. Int J Selected invited lectures Environ Anal Chem 90 (2010), 620. 2. Hamer B, Medaković D, Pavičić-Hamer D, 1. Lyons DM. Nanobiotechnology at the Ruđer Jakšić Ž, Štifanić M, Nerlović V, Travizi A, Bošković Institute, Nano-biotechnology Inter- Precali R, Kanduč T. Estimation of Freshwa- national Workshop, Institute for Health and ter Influx Along Eastern Adriatic Coast as a Consumer Protection, European Commis- Possible Source of Stress for Marine Organ- sion Joint Research Centre, Ispra, Italy, No- isms. Acta Adriatica 51 (2010), 181. vember 30–December 02, 2010. 3. Mozetič P, Solidoro C, Cossarini G, Socal G, 2. Medaković D. Some ecological events in the Precali R, Francé J, Bianchi F, De Vittor C, North Adriatic Sea, TBT contamination. Fach- Smodlaka N, Fonda Umani S. Recent Trends hochschule Technikum Wien, Wien, Austria, Towards Oligotrophication of the Northern December 18, 2010. Adriatic: Evidence from Chlorophyll a Time Series. Estuar Coasts 33 (2010), 362. 4. Hernández-Becerril D, Viličić D, Bosak S, Awards and appointments Đakovac T. Morphology and ecology of the diatom Chaetoceros vixvisibilis (Chaetoc- Daniel M. Lyons: RBI Director’s Award for erotales, Bacillariophyceae) from the Adriatic Scientific Excellence for winning a competi- Sea. J Plankton Res 32 (2010), 1513. tive international or domestic project worth 5. Ivančić I, Fuks D, Radić T, Lyons DM, Šilović more than 500.000 kn. T, Kraus R, Precali R. Phytoplankton and Tina Šilović: Uncovering composition and Bacterial Alkaline Phosphatase Activity in the diversity of marine picophytoplankton in north- Northern Adriatic Sea. Mar Environ Res 69 ern Adriatic coastal waters (UKF, grant 2A) (2010), 85. 6. Nygren A, Sundkvist T, Mikac B, Pleijel F. Two new and two poorly known autolytines (Poly- Organization of chaeta: Syllidae) from Madeira and the Medi- conferences, congresses terranean Sea. Zootaxa 2640 (2010), 35. and meetings 7. Iveša Lj, Chapman GM, Underwood A, Mur- phy RJ. Differential patterns of distribution JOINT Workshop / MC Meeting, COST of limpets on intertidal seawalls: experimen- Action TD0903, Understanding and manipu- tal investigation of the roles of recruitment, lating enzymatic and proteomic processes in survival and competition. Mar Ecol- Prog Ser biomineralization, May 20-22, 2010, Pula, 407 (2010), 55. Croatia (Davorin Medaković) RBI Annual Report 2010. RBI

86 OVERVIEW OFTHEDIVISION tion Communication Technology—ICST.tion technologies on the basis of Science Informa- have resulted in the development of eScience computer technologies, informatics, which modern on based research, scientific (global) multidisciplinary (RBI)andmulti-institutional technology andlogisticsforcontemporary ment andmaintenanceoftheinfrastructure, formation technologyandcomputing. in- optoelectronics, of fields the in performed ment programs and services for scientists are Institute inwhichresearchprojects,develop- is an organizational unit of the Ruđer Bošković partments: de- (CIC) consistsofthefollowing ing The CentreforInformaticsandComput- Head: KaroljSkala CENTRE ORGANISATION OFTHE and Computing Centre forInformatics Mission: Scientific research, develop- research, Scientific Mission: The CentreforInformaticsandComputing a a a a

IT ServicesDepartment systems, Department forInformation systems, Department forR&DofICST Laboratory, Optoelectronics andVisualisation ronment and a scientific basis for his arrival to Afgan to realize two aims: (1) provide an envi- and establishmentofacollaboration withEnis been contractedwiththe EC.Reintegration Environments (AIS-DC) for DistributedComputing Services Application Information EU FP7projects: completed three CIR successfully EUprojects Completed three TOP ACHIEVEMENTS This FP7 People Reintegration Grant has This FP7 People Reintegration Grant has competence centre. the aim of establishing a national virtual as DARIAcontinues DARIAH ERICwith 3. 2. 1. Arts andHumanities(DARIAH). Infrastructureforthe Research Digital for theconstructionof Preparing (SEE-GRID SCI), eInfrastructure forregionaleScience (EGEE-III), Enabling GridsforE-science-III http://www.irb.hr/en/cir

87 2010. Report Annual RBI CIR 88 RBI Annual Report 2010. CIR PROJECTS ANDPROGRAMS 3. 2. 1. AWARDS Education of thescientificresearchinfrastructure. facilitate long-termsustainabilityandgrowth as an eScience computing paradigm that will oping andpromotingasetofcloudservices RBI; and(2)formulateaframeworkfordevel- toral studies. in 3doc- of Zagreb.CIRalsoparticipated the FacultyofGraphic Arts attheUniversity as at as well Computing and cal Engineering coursesattheFaculty ofElectri- 3 graduate n ad iulsto (0721) Karolj Skala (2007-2012), Visualisation and ing presented awardtodr. Skala Fig. 1.PresidentoftheHungarian Academy ofScience Employees provided 5 undergraduate and Employees provided5undergraduate Scientific program: Distributed Comput- Distributed program: Scientific successful realisationofEUFP7projects. Karolj Skala:theRBIDirector’s Award for Egg” Fig2 Tesla’s “Golden Award e-Novation VIDI CIR lab(Skala,Lipic,Gjenero,Grubisic): Science. Fig1 Medal from the Hungarian Academy of Karolj Skalareceivedthe Arany Janos 1. CONFERENCES SELECTED ORGANIZED been severalearlierrelatedprojects. have there Computing, for Informaticsand tested. WithintheRBICentre been tion have and interactions andinterac- many methodsofvisualisation human-machine of field cal aswellpractical,relatedtothebroad base,theoreti- produced alargeknowledge has by projectassociates of theresearch teractive workinvirtualandrealspace.Much ence inintegrationofuserinterfacesand- experi languages, procedural and scriptive pre- offormaldescriptive, application and development interfaces, visualperipherals, algorithms, visual puter graphics,parallel com- processing, signal work, in past project through experience has group research The abroad. and in Croatia both development, various theoreticalandappliedresearch Methods (2007-2011), KaroljSkala Fig. 2.FirstprizeVIDIeNovationGolden Tesla’s Egg This project’s associates haveperformed Visualisation Scientific project: Scientific 33 chair K.Skala. 24 Monday Croatia, Opatija, (GVS), SYSTEMS VISUALISATION AND GRID on FERENCE th th - Friday 28 CON - MIPRO, Convention International th of May 2010, conference - 2. 1. SELECTED PUBICATIONS Earth SciInform3(2010),199. and forecastingmodel. research weather Prtenjak M: of Grid implementation the Telišman- D, Belušić K, Skala D, Davidović (2010), 173. FutureGenerCompSy ing environments. information servicesfordistributedcomput- P,Afgan E,Bangalore SkalaK: Application 27 4. 3. dioelectronics &Informatics time characterization.Ra- using propagation I: Metastability testingatFPGA circuitdesign B, Medved Rogina ŠkodaP, Skala K, Michieli Supercomput Execution of Loosely Coupled Applications. P,Afgan E,Bangalore Skala T:Job Planning 58(2010),460. 51(2010),4. J

89 2010. Report Annual RBI CIR RBI Annual Report 2010. RBI

90 O trolytes inlithiumcells. ducting glassesas candidates for solid elec- attention hasbeendevoted tolithiumcon- Special biomedicine. and devices chemical technology,waste use innuclear tial electro- phate glassesandglass-ceramicsfor poten- phos- of novelelectronic–ionic development strategic projects. several the These include pharmaceutical industry. searchers fromgovernmentinstitutionsand to re- support and professional educational provides also Centre The Osijek. and Split of Zagreb,Rijeka, tute andtheUniversities and researchers at the Ruđer Bošković Insti- troscopy andprovideservicesto scientists spec- NMR of field the in research scientific verview oftheCentre following laboratories: consistsofthe The Centre Head: DejanPlavšić Centre Organization ofthe Magnetic Resonance Centre forNuclear The Glass Group has been involved in The GlassGrouphasbeeninvolved The missionoftheCentreistoconduct a a

Milanković Glass Laboratory,Moguš- Andrea and modelling,DejanPlavšić for NMRspectroscopy Laboratory et al.,2010). (Moguš-Milanković conductivity ionic cating indi of magnitude orders five by increases 40 mol % thevalues of electrical conductivity electrical conductivity at20mol%Li electrical in glassnetwork. The observed minimumin bonds oxygen the of nature the in changes of Li of andelectricalproperties Structural surpass α-MSH(Blagaićetal.,2010). model this in γ-MSH and β- of efficacy and APAP-induced liverlesionsinmice. Potency hepatoprotective substancesthat prevent stimulating hormones(MSH)represent and γ-MSH α-,β-, Hepatoprotective of effects T network. With increasing Li network. Withincreasing of to the glass the structuralreorganization properties at20mol%Li op achievements It was shown that α-, β, and γ-melanocyte The systematic change in the variety of 2 O-ZnO-P 2 O 5 glasses http://www.irb.hr/en/nmr 2 O is explained by explained is O 2 O content up to up content O 2 O is due -

91 2010. Report Annual RBI NMR 92 RBI Annual Report 2010. NMR A Education New equipment ties ofIronPhosphatePolaronGlasses. Impact on MagneticProper- Crystallization Science Foundationforresearchon National ship for doctoral students from the Croatian Excellence and Luka Pavić obtained a fellow ceived the RBI Director’s Award for Scientific Centre staff member. second byanoutsidestudentmentoreda by astaffdefended has been member,a and thesis PhD One Rijeka. and Dubrovnik jek, Osi- Split, Zagreb, of Universities at courses and 8postgraduate taught 2undergraduate installed intheGlassgroup(Fig.1.). pha A-high Performance Analyzer) hasbeen mance Analyzer). Fig. 1.Impedance Analyzer (Novocontrol Alpha A-high Perfor- wards Dejan Plavšić and Dražen Vikić-TopićDražen re - and Plavšić Dejan In 2010, themembersofCentre Al- (Novocontrol Analyzer Impedance An - international projects international Research, developmental and 4. 3. 2. 1. Science,Education andSports of bytheMinistry supported Projects PROJECTS 2. 1. SELECTED PUBICATIONS 1. CONFERENCES ANDCOURSES SELECTED ORGANIZED 3. 2. 1. Štambuk ing of their propertiesandactivity, Nikola and test- of bioactivemolecules Modelling tive molecules,DejanPlavšić of bioac modelling NMR spectroscopyand Moguš-Milanković proper ties of(bioactive)glasses/ceramics, Andrea onelectrical structure of Influence chemistry,Graovac Ante and computational methods ofmathematical and materialsby of molecules Modelling ojvd P BbnBaać , eaa T, Kos M, Aralica G, Čulo Kelava F. The Influence of α-, A, Boban-Blagaić P, N, Konjevoda Štambuk P, Turčić K, Houra V, Blagaić 31 (2010),1832. matrix. distance graph Novel D. Plavšić M, Novič T, Pisanski M, Randić The 25 Slovenian project,MarijanaVinković II Receptor Croatian- Antagonists, bilateral NMR StudyoftheInteractions Angiotensin Milanković Moguš- Andrea project, Croatian-Slovenian bilateral glass, bioactive of relaxation lectric die- and mobility of electrical Investigation man project, Andrea Moguš-Milanković phosphate glasses,bilateralCroatian-Ger New insightsintochargetransportiniron tia, July7-12,2010. MATH/CHEM/COMP 2010, Dubrovnik, Croa- th InternationalCourse&Conference J CompChem - - - 4. 3. erties ofLi prop- electrical M. Structuraland DE, Ivanda Day ST, Reis L, Pavić A, Moguš-Milanković (2010), 9405. [3.2.1] octadiene Derivatives.Tetrahedron Furobenzobicyclo Styryl-substituted Novel Šindler-Kulyk Ž, M. SynthesisandPhototransformationsof Marinić I, Škorić I, Kikaš Male CBA Mice.Molecules on Induced LiverLesionsin Acetaminophen Hormone Stimulating γ-Melanocyte and β-, Cryst Solids356(2010),715. 2 O-ZnO-P 2 O 5 glasses.JNon- 15(2010),1232.

66 5. 6. Fe atć , i C, ebr D, Moguš- DE, Cr of Delbert properties Electrical A, Milanković CW, Kim A, Šantić (2010) 28. QSAR of flavonoids. antiradical in values TEAC experimental and by thePM6method calculated tion enthalpy dissocia bond of Reliability B. Lučić D, Amić Cryst Solids356(2010),2699. 2 O 3 -P 2 O 5 glasses.PartII. Bioorgan Med Chem Bioorgan Journal ofNon- 2 O 18 3- -

93 2010. Report Annual RBI NMR RBI Annual Report 2010. RBI

94 Joomla was chosen. Library staffLibrary was chosen. Joomla managed software source the open website, the new new andmodernservices forourusers. functionalities, visualdesign, andtoprovide architecture, information it inordertoimprove andredesign cided tocompletelyreorganize in 2010 wede- developing, was continuously visits monthly. website the library Although demic community, and hadaround200.000 the researchandaca- inside very popular website backin April 1994. This websitewas Web asanew media andlaunchedtheirown Croatia which recognizedthe potential of the speeded upscholarlycommunication. knowledge. and improved All thesechanges communicating withpeers,andsharingtheir of information,potentialbusinesspartners, time ontheWeb,types for various searching more and Researchers areinvesting expectations, and goals and in the services they provide anduse. their in research outputs, quality-assured to access viding and pro- and librariesplayindisseminating ing bodies,researchinstitutions,publishers, changes intherolesthatresearchers,fund- munication. Technological changes caused Web,of the com- istransformingscholarly tion technology,the rapidgrowth especially Website redesign ACTIVITIES O Head ofLibrary:BojanMacan VER Library As a content management systemfor As acontentmanagement in library first the was Library RBI The The fast pace of in informa - development VIEW OFLIBRARY room inthe1 server central equipped newly a to servers was launchedattheendofyear. port. website Library The newlyredesigned sup- external any without design, visual and mation architecturedesign,contentcreation and setup,infor- installation solutions, ware soft- and testingofavailable the selection During 2010 the acquisition, cataloguing and of library management. Koha intoallspheres of anintegratedlibrarysystem plementation management library for ILSasaplatform Open source Fig. 1: The newLibrarywebsite In 2010theLibraryalsomovedallofits The Library continued the processofim- The Librarycontinued st wing. http://library.irb.hr

95 2010. Report Annual RBI LIB 96 RBI Annual Report 2010. LIB to the Croatianacademic community. The Li- e-books available as well42biomedical and naturalsciences databases, biomedical 30 freeand fee and around free e-journals, Collection Developement public in2012. pository, which will be presented to the wider re- institutional RBI’s the in articles text full of digitized andavailablealongwithanumber available issuesofRuđer tion project, all RBI’s annual reports and all browse functionalities. As partofthedigitiza- tographs whichwillenablevarioussearchand through detailedmetadatadescriptionofpho- galleries andprovideadditionalfunctionalities future theLibraryisplanningtomergetwo tains new, digitallybornphotographs.Inthe con- and Office Relations Public RBI’s the by galerija/). This photogalleryisadministrated at theRBItopublic(https://lib.irb.hr/foto- photo gallerywasimplementedtopresentlife tication (http://lib.irb.hr/fotoarhiv/). Another IP rangevalidationor [email protected] authen- gallery, andvisibleonlytoRBIstaff through digitized and archived to the Library’s Photo As aresultmorethan10,000photographsare plementation oftheRBI’s DigitalRepository. rials abouttheInstitutewithinprojectofim- digitizing graphicandotherdocumentalmate- digital repository digitalinitiatives –central Library pletely automatedcirculationprocess. books for the purpose of faster and com- enabled barcodingthe printed collectionof in the Librarywhich labels wasdeveloped ed librarysystem. schema andimportedintothenewintegrat- metadata verted intoMARC21bibliographic existing library thesis records ware also con- to Koha. migrated completely ses ware The circulation of printed ande-booksthe- In 2010 therewerearound16,000fee and of process the finished has Library The barcode forgenerating An application magazine are also magazine arealso room inthe5 reading library the in exhibited permanently and ited byRBI researchers wasorganized M.Sc. theses. umes of Ph. D. theses and 420 volumesof vol- 443 included of theses in2010 collection The acquired. were books new 314 of total year,the During acquisition. books of a cess oftimeandmoney inthepro- great savings ensured which purchasing card foronline new database–Facultyof1000. as wellone subscriptions, as institutional (mostly in additionto e-version of journals) and 49printedjournals to 102e-journals tion forRBI. In 2010theLibrarysubscribed manage e-journalande-databasessubscrip to continued the Library subscriptions, tional na- Besides Zeitschriftenbibliothek. ronische engine Pero,aswellthroughEZB Elekt- madesearch viaanin-house able e-journals brary continuedto provide accesstoallavail library Ser publications. 24 new donated RBI researchers year 2010 relevant scientificandother informationare: services whichprovide and easeaccessto research community. Some of the information users andthewholeCroatian academicand formation servicesandprojects,bothforRBI in- existing of maintenance and development Fig. 2:ExhibitionofpublicationswrittenoreditedbytheRBIstaff. A writtenored- ofpublications collection In 2010theLibrarywasgrantedacredit In 2010 the Library was focused on the In 2010theLibrarywasfocusedon th wing. For this collection in the wing.Forthiscollection vices - - Institute werealsoprovided. analyses forvariouspurposes forthewhole used. Differentwere Contents bibliometric Scholar,pus, Google SPIRESandCurrent Webdatabases liographic ofScience,Sco- employees. Forthispurposecitationandbib- RBI for certificates citation 85 issued Library metric servicesforRBIscientists.In2010the biblio as providing ence database,aswell Sci- of Web the in indexed as affiliation as and updatethedatabaseof papers withRBI Bibliometric analysis to collaborateasapartofHRČAKteam. continued SRCE, andtheRBILibrary Centre Computing by theUniversity maintained was HRČAK journals access open Croatian visits perday.than 20.000 of The repository services withmore information of thepopular of themajority cal supportandmaintenance techni- provided work theLibrary voluntary Compasses). Withgreateffort and alotof of (APair ŠESTAR equipment scientific of (http://www.znanstvenici.hr Portal Scientific Croatian Croatia, in Science in Who Who’s Databases, Online for Centre opment of information services like CROSBI, Sports almost halted thecontinuousdevel the MinistryofScience,Educationand through thenewapplication. of RBIauditoriums to administerreservation hall reservationsandtheLibrarycontinued oped a new reservationsystem for lecture matics andcomputing,theLibrarydevel • • • • • The Library has continued to The Libraryhascontinued maintain from support in reduction significant The the Centreforinfor- with In collaboration IRB reservations(https://rezervacije.irb.hr) research libraries(http://knjiznice.szi.hr) CMS forwebsitesofCroatianacademicand online-baze.hr) (http://www. Databases Online for Centre tkojetko.irb.hr) Who’s WhoinScienceCroatia(http:// ( Croatian Scientific Bibliography – CROSBI http://bib.irb.hr) ) andrepository - - - Fig. 3:ShortEducationalLibrarySeminars (KEKS) Education 455 (87%)ofthoserequests. resolved and positively er Croatianlibraries, 526 requests for document delivery from oth- free ofcharge(74%). acquired were requests of resolved majority positively The (22%). SUBITO by lowed (33%), fol- through theIAMSLICcatalogue tively resolved.Most items were obtained posi- were requests those of 96% and staff RBI by delivery document for requests 896 are charged.In 2010 the RBI library received copy and loan requests from libraries abroad are mainlyfreeofcharge,while libraries braries as well as from EURASLIC/IAMSLIC library loanservicesfromotherCroatianli- with librariesfromCroatiaandabroad.Inter- service and lending loan interlibrary lished collection were overcome witha well-estab loan Interlibrary (max 10 participants) in one of the Library’s the of one in participants) 10 (max inars are in smallgroups held periodically and ahalfortwohoursworkshops. The sem- of shortlectures(45minutes)andanhour seminari inCroatian). The seminars consist knjižnični edukacijski Kratki Seminars, brary Li- seminars (acronymofShortEducational In addition, the RBI library alsoreceived theRBIlibrary In addition, of theRBIlibrary The inherentshortages The LibrarystartedanewseriesofKEKS -

97 2010. Report Annual RBI LIB reading rooms. Thirty five seminars were to RBI staff and the general public. During held in 2010 with 178 participants. this year seven lectures were organized.

LIB Library staff was also involved in undergraduate and graduate level teaching at the J. J. Strossmayer University in Osijek and PROJECTS the University of Zadar. In 2010 Library applied as a partner in the International cooperation FP7 project called 2nd Generation Open Access Infrastructure for Research in Europe - Ope- The Library continued its international nAIREplus (under the Infrastructure Call 9). cooperation with EURASLIC (European As- sociation of Aquatic Sciences Libraries and Information Centres) and its parent organization IAMSLIC (European Association of Aquatic Sciences Libraries and Information Centres). Library staff had an active role in EURASLIC activities: two members in the EURASLIC board, as well as in the sub- groups ECET (European Countries in Eco- nomic Transition) group and MedSig group (The Mediterranean Special Interest Group). As part of its involvement, library staff par- Fig. 4: Library’s presentation point “A Fistful of Books” at the 2010 ticipated in training courses held at the UN- RBI Open Days ESCO/IOC Project Office for IODE in Oost- ende, Belgium. The Library was also actively SELECTED publications involved in IAMSLIC as a member of the Strategic Planning Group. 1. Pažur I, Macan B: KEKS - a short educational library seminars, an example of user education Organization of events at Ruđer Bošković Institute Library in Zagreb. Vjesnik bibliotekara Hrvatske 53 (2010), 73. The Library was actively involved in the 2. Stojanovski J: Metrics of scientific journalism organization of RBI Open Days, held in May - truths, myths and misconceptions. Kem Ind and participated in the programme with a 59 (2010), 179. presentation point „A Fistful of Books“. In this 3. Grgić M, Macan B: Impact Factor and scien- way the Library presented its activities and tific promotions. Kem Ind 59 (2010), 128. informed visitors about various information 4. Stojanovski J: 60 Years of the Ruđer Bošković resources available on the Internet. Institute Library. Kem Ind 59 (2010), 608. Library staff was also actively involved in 5. Pažur Vojvodić I: Chemistry on Facebook. organization of another important event in Kem Ind 59 (2010), 80. 2010 – the RBI 60th anniversary celebration. At the central celebration held at the Vatro- slav Lisinski Concert Hall, the Library suc- SELECTED LECTURE cessfully presented its activities through 3 poster presentations. 1. Macan B, Fernandez GV. ABCD vs. Koha: During the year the Library also continued open source library options. 36th IAMSLlC to organize the regular RBI Library Colloquia. Annual Conference: netting knowledge: two RBI Annual Report 2010. RBI Colloquia topics were broad and chosen to hemispheres - one world. Mar del Plata, Ar- be of interest not only to librarians, but also gentina, October 17-21, 2010.

98 99 2010. Report Annual RBI LIB