ENO3 Antibody Cat. No.: 30-377

ENO3 Antibody

Antibody used in WB on Human HepG2 at 5.0 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human ENO3.

TESTED APPLICATIONS: ELISA, IHC, WB

ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody APPLICATIONS: can be used for detection of ENO3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate

PREDICTED MOLECULAR 47 kDa WEIGHT:

September 24, 2021 1 https://www.prosci-inc.com/eno3-antibody-30-377.html Properties

PURIFICATION: Antibody is purified by protein A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store ENO3 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: ENO3

ALTERNATE NAMES: ENO3, MSE, GSD13

ACCESSION NO.: NP_001967

PROTEIN GI NO.: 301897469

GENE ID: 2027

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

ENO3 is one of the three isoenzymes found in mammals. This isoenzyme, a homodimer, is found in cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 can be associated with metabolic that may result from decreased stability of the .This gene encodes one of the three enolase isoenzymes found in BACKGROUND: mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

REFERENCES: 1) Li, T.B., (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36 (6), 412-418.

ANTIBODIES FOR RESEARCH USE ONLY.

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