ENO3 Antibody Cat. No.: 30-377
ENO3 Antibody
Antibody used in WB on Human HepG2 at 5.0 ug/ml.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human ENO3.
TESTED APPLICATIONS: ELISA, IHC, WB
ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody APPLICATIONS: can be used for detection of ENO3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate
PREDICTED MOLECULAR 47 kDa WEIGHT:
September 24, 2021 1 https://www.prosci-inc.com/eno3-antibody-30-377.html Properties
PURIFICATION: Antibody is purified by protein A chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ENO3 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: ENO3
ALTERNATE NAMES: ENO3, MSE, GSD13
ACCESSION NO.: NP_001967
PROTEIN GI NO.: 301897469
GENE ID: 2027
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in BACKGROUND: mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.
REFERENCES: 1) Li, T.B., (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36 (6), 412-418.
ANTIBODIES FOR RESEARCH USE ONLY.
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