Product Datasheet Anti-Fox2 / RBM9 antibody ab57154

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Overview

Product name Anti-Fox2 / RBM9 antibody Description Mouse monoclonal to Fox2 / RBM9 Tested applications WB, IHC-P, ICC, Flow Cyt, IHC-FoFr Species reactivity Reacts with: Mouse, Human

Immunogen Recombinant fragment: MEKKKMVTQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPT EYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQN GSLTTEGGAQ TDGQQSQTQS , corresponding to amino acids 1-100 of Human Fox2/ RBM9 Run BLAST with Run BLAST with Properties

Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: None PBS, pH 7.2 Purity G purified Clonality Monoclonal Isotype IgG2a Light chain type kappa Research Areas Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing Applications

Our Abpromise guarantee covers the use of ab57154 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes WB WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa. IHC-P IHC-P: Use a concentration of 3 µg/ml. ICC ICC: Use a concentration of 1 µg/ml.

Flow Cyt Flow Cyt: Use 1µg for 106 cells. IHC-FoFr IHC-FoFr: Use at an assay dependent concentration. Target

Function RNA-binding protein that regulates events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. Sequence similarities Contains 1 RRM (RNA recognition motif) domain. Cellular localization Nucleus. Cytoplasm.

http://www.abcam.com/Fox2--RBM9-antibody-ab57154.html 1 Product Datasheet Target information above from: UniProt accession O43251 The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010) .

Database links : 23543 Human Entrez Gene: 93686 Mouse Omim: 612149 Human SwissProt: O43251 Human SwissProt: Q8BP71 Mouse Unigene: 282998 Human Unigene: 202774 Mouse Alternative names HRNBP2 antibody Fox 1 homologue antibody Fox-1 homolog B antibody FOX2 antibody FXH antibody Hexaribonucleotide-binding protein 2 antibody HNRBP2 antibody RBFOX2 antibody Repressor of tamoxifen transcriptional activity antibody RFOX2_HUMAN antibody RNA binding motif protein 9 antibody RNA binding protein 9 antibody RNA binding protein fox-1 homolog 2 antibody RNA-binding motif protein 9 antibody RNA-binding protein 9 antibody RTA antibody Anti-Fox2 / RBM9 antibody images

Fox2 / RBM9 antibody (ab57154) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.

IHC-P - Fox2 / RBM9 antibody (ab57154)

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Predicted band size : 41 kDa Fox2 / RBM9 antibody (ab57154) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

Western blot - Fox2 / RBM9 antibody (ab57154)

ICC/IF image of ab57154 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57154, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. Immunocytochemistry/ DAPI was used to stain the cell nuclei (blue) Immunofluorescence-Fox2 / RBM9 at a concentration of 1.43µM. antibody(ab57154)

Overlay histogram showing HeLa cells stained with ab57154 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS- Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57154, 1µg/1x106 cells) for 30 Flow Cytometry - Anti-Fox2 / RBM9 min at 22ºC. The secondary antibody used antibody (ab57154) was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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