TESTS FOR NEW TECHNOLOGY

In August 2009 we commissioned new allergy test systems that further extend our testing menu. Our new technology detects Specifi c IgE to clinically important individual molecules. We continue to offer testing for to whole food and inhalant allergens. The main analyser platforms and reagent systems are the Phadia ImmunoCAP 250, ImmunoCAP 1000 and ISAC ImmunoCAP. Our comprehensive menu is included in this brochure. You may request the allergens using their alphanumeric codes. Please precede these codes with “Specifi c IgE for”, or “Allergy serology for”, or “RAST for”.

allergen specifi c IgE values in kU/L and a should offer the best available allergy testing semiquantitative class value. Please note systems and give patients the opportunity that some patients with a “negative” or “0” to cover the cost of more defi nitive higher class may have detectable traces of specifi c quality testing that the government will not IgE that can have clinical signifi cance as meet. In May 2009, the Medicare Benefi ts a marker of early sensitisation or previous Schedule reduced rebates for Allergy signifi cant sensitisation to insect venoms testing by 1%: a reimbursement that is now and drugs. In the most important practical less than 20% of rebates in comparable advance in laboratory allergy diagnostics, countries for laboratory allergy testing. It the Phadia ImmunoCAP ISAC system now is almost impossible to answer a simple, allows us to determine a patient’s specifi c IgE sensible laboratory allergy testing request to 103 clinically important allergen molecules within the constraints of the level of Medicare from 47 different allergens. Tests that defi ne reimbursement. Consequently, the laboratory the molecule in a food to which a patient is unable to accept Medicare reimbursement has signifi cant specifi c IgE, can provide for more than very basic allergy testing Dear Colleague, diagnostic and prognostic information requests. In this menu, we seek to give for patients with serious . Our your patients clear options and choices Welcome to our new Allergy Test Menu, one new technology allows us to do this with when it comes to allergy testing. You and of the largest internationally available allergy recombinant ImmunoCAP allergens (page your patients will be pleased to know that testing menus. All our specifi c IgE testing is 10) or as a profi le with the ISAC system although some new allergens and mixes are now performed on the Phadia ImmunoCAP (page 11). We continue to offer other tests more expensive, core individual allergens and 250 and Phadia ImmunoCAP 1000 system important for assessing allergy problems: extended panels are cheaper than previously. (pages 3-10). An advantage of the Phadia Total IgE, Tryptase, Eosinophilic Cationic Please send email enquiries to ImmunoCAP system is that, for certain key (ECP), C1 esterase and genetic foods, the 95% probability of a positive [email protected] or testing for Hereditary Types I, II Phone: 02 98 555 286 food challenge has been determined for and III (see next page). certain levels of allergen-specifi c IgE (see Karl W Baumgart Table below). We will continue to report We have always taken the view that we Director, Immunology

ALLERGEN SPECIFIC IgE ORDERING ALLERGY TESTS

We ask that you specifi cally nominate which allergens you Specifi c IgE Levels Conferring Very High Risk would like tested. Please do not write the allergens to be tested in the clinical notes section of the request form. A ALLERGEN kU/L PPV comprehensive menu of allergens that we stock and test for is available in the following pages. If allergens are not available Egg (<= 2yo) 2 95% we may use cross-reactive ones or advise you on the report. Egg (> 2yo) 7 98% Some “allergy symptoms” may result from intolerance mechanisms (salicylates, amines, MSG, metabisulphite) Milk (<=2 yo) 5 95% and detection of IgE to them is not useful or possible. You Milk (>2 yo) 15 95% can request the allergens using their alphanumeric codes. To assist staff performing data entry, we appreciate it if you Peanut 14 100% precede these codes by “Specifi c IgE for” or “Allergy serology Fish 20 100% for” or “RAST for”. Tree nuts ~15 ~95% If you simply write “RAST” we will now test as follows: Child 6 years or less: dust mite, , cow’s milk, 26 74% peanut and soy (Panel A1) Adult or child over 6 years: dust mite, cat, dog, grass This table was derived from several large studies in which and alternaria (Panel A2) patients were challenged with the food. The positive predictive If you write “food and inhalant allergens”, we will test a staple value (PPV) of a specifi c IgE level for a positive challenge food mix (Fx5, that includes egg white, cow’s milk, peanut, was determined. It illustrates that specifi c IgE levels for soy, wheat and codfi sh), dust mite, fescue grass pollen-which individual allergens are like “international currencies” and are cross-reacts with almost all other grasses and, alternaria-an not necessarily comparable for a particular level for all the outdoor mould with small spores that can easily be inhaled allergens. It defi nes levels at which a challenge, or exposure, into the small airways (Panel A16). We do recommend some would be highly hazardous for a patient. Importantly, many extended panels of individual allergens and can specifi cally patients could have serious reactions at much lower levels. design panels for you and your patients.

aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 1 117/03/107/03/10 1:151:15 PMPM TOTAL IgE Normal total IgE levels (< 100 kU/L) do not exclude significant allergies. Patients with normal total IgE levels can still be allergic to venom, dustmite, and many other allergens. Significant specific IgE levels are extraordinarily rare in individuals with total IgE values of less than 7 kU/L. Persons with very elevated total IgE levels are more likely to have significant elevations in specific IgE. Very elevated levels of total IgE also occur in persons with severe eczema, helminthic and parasitic infestations as well as allergobronchopulmonary aspergillosis (ABPA). Monitoring total IgE levels is useful in patients on immunosuppression for their severe eczema as well as in ABPA.

TRYPTASE Elevated levels of mast cell tryptase in peripheral blood indicate systemic mast cell degranulation or an increased number of mast cells which can occur in patients with mastocytosis. Tryptase is NOT ELEVATED in persons with from foods or non-parenteral agents. Elevated levels indicate an adverse reaction to a stinging insect or a reaction to an intravenous, intramuscular or subcutaneous diagnostic or therapeutic agent. Any elevated tryptase levels should be repeated after a period of time (3-10 days) to document a return to normal levels. Persistently elevated levels of tryptase are strongly suggestive of systemic mastocytosis which may not always manifest with urticaria pigmentosa. Since mastocytosis is more common in persons with stinging insect reactions, persons who have had systemic reactions to stinging insects should always have their tryptase level checked together with relevant venom specific IgE. The test is performed on serum. Normal values are < 13.5 ug/L. For these indications, the test is Medicare rebatable.

EOSINOPHILIC CATIONIC PROTEIN (ECP) ECP is elevated in persons with active eosinophilic inflammation. It is a useful marker in the diagnosis and management of hypereosinophilic disorders. ECP is also a useful marker of allergic airways inflammation in young children. The test is performed on serum. Normal values for children are < 20 ug/L and adults < 15 ug/L. At present the test is only reimbursed by Medicare when requested in children under 12 years of age for assessment of airways inflammation (Please write “” on clinical notes). For all other requests, the fee is $50, non-rebatable.

Type I & II (C1 ESTERASE INHIBITOR) HEREDITARY ANGIOEDEMA This should be considered in anyone with a family history of angioedema without urticaria. C1 esterase level and function, C3 and C4 should be measured at the same time to facilitate interpretation of the results. Abnormal levels occur in persons with deficiency which has autosomal dominant inheritance. Persons with this disorder usually experience symptoms from puberty onwards and may have life-threatening episodes which are not responsive to adrenaline. Many patients also experience recurrent abdominal pain due to visceral angioedema. Functional deficiency may occur on a genetic basis or in older persons it can result from an interfering autoantibody associated with lymphoma. Abnormal functional assay results should always be repeated. The C3, C4, quantitative and functional C1-esterase (C1-inhibitor) assays are performed on serum and reimbursed by Medicare. Sequencing for the detection of mutations causing Type I and II HAE is performed by the laboratory and the fee is $395 (non-rebatable).

TYPE III (FACTOR XII) HEREDITARY ANGIOEDEMA This should also be considered, mainly in women, who have angioedema without urticaria. Type III (Factor XII) Hereditary Angioedema is detected by a real-time PCR assay which detects the most common mutation in Factor XII. This is a cause of angioedema without urticaria in women. This is the only assay for this disorder which is thought to be at least as common as Type I and Type II HAE. At present there is a non-rebatable fee of $120. The laboratory can also perform sequencing of Factor XII if required. The fee is $395 (non-rebatable).

INITIAL INVESTIGATION PANELS A1: CHILD 6 years or less A2: ADULT, or CHILD over 6 years If you write “RAST” or “Allergy serology”, but do not specify the allergens, Total IgE Total IgE we will perform the tests listed under A1 (for a child 6 years or less) or A2 (Adult, or child over 6 years). Any subsequently requested additional D1 Dustmite D1 Dustmite allergens are charged according to the billing policy listed below. F1 Egg white G4 Grass pollen (Fescue) F2 Cow’s milk E1 Cat F13 Peanut E5 Dog F14 Soy M6 Alternaria

OTHER COMMON INITIAL INVESTIGATION PANELS * Please write the name and code of the panel on the request form. One (1) of our panels is within our allowance under Medicare

A4 ANIMAL INHALANTS A5 RURAL INHALANTS A6 PETS A7 MOULDS E1 Cat G17 Paspalum E1 Cat M2 Cladosporium E5 Dog WX1 Weed mix E5 Dog M3 Aspergillus D1 Dustmite MX1 Mould mix E6 Guinea pig M5 Candida albicans G4 Grass pollen E82 Rabbit M6 Alternaria

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 2 117/03/107/03/10 1:161:16 PMPM OTHER COMMON INITIAL INVESTIGATION PANELS * Please write the name and code of the panel on the request form. One (1) of our panels is within our allowance under Medicare

A8 NATIVE TREES A9 FOOD SCREEN A10 NUTS A11 SEAFOOD T18 Eucalyptus FX1 Nut Mix F13 Peanut F3 Codfish T19 Acacia F23 Crab F17 Hazelnut F23 Crab T21 Melaleuca F4 Wheat F20 Almond F24 Prawn T73 Australian Pine F1 Egg F201 Pecan nut F80 Lobster

A12 CEREALS A13 STAPLE FOODS A14 INSECTS A15 LATEX & FOODS F4 Wheat F1 Egg white I1 Honey bee venom K82 Latex F6 Barley F2 Milk I4 Paper venom F84 Kiwi fruit F9 Rice F13 Peanut I3 Yellow Jacket F87 Melon F14 Soy F14 Soybean I70 Fire ant venom F92 I71

A16 FOOD & INHALANTS FX5 Staple Foods G4 Grass Pollen (fescue) D1 Dust Mite M6 Alternaria

A3 CHILD ALLERGY ASSESSMENT PANEL (REQUEST “IgE AND RAST A3”) This new panel is designed for the assessment of children at high risk of allergic disease, especially those with eczema, possible food and inhalant . The cost of this panel is $108, for which a Medicare rebate of $22.95 may be available.

D1 Dustmite M6 Alternaria alternata F202 Cashew E1 Cat Epithelium F1 Egg White F17 Hazelnut E82 Rabbit Epithelium F75 Egg F35 Potato E5 Dog F2 Milk F84 Kiwi Fruit E3 Horse Dander F3 Codfi sh F91 G2 Bermuda Grass F14 Soybean F92 Banana G4 Grass Pollen (fescue) F4 Wheat K82 Latex G5 Perennial Rye Grass F10 Seed M80 Staph. Enterotoxin A W21 Parietaria judaica F13 Peanut M70 Pityrosporum orbiculare

EXTENDED ALLERGEN PANEL EXAMPLES * Our charge for each of these panels is $80, for which a rebate of $22.95 may be available per episode. Each allergen may also be ordered separately by its code or name. Other personalised extended panels are available on request. Please call Dr Karl Baumgart to discuss your requirements.

A20 INHALANTS A21 MOULD & STORAGE MITES A22 FOODS A23 ANAPHYLACTIC FOODS D1 Dustmite M1 Penicillium notatum F1 Egg White F3 Codfish E1 Cat Epithelium M2 Cladosporium herbarum F2 Milk F10 Sesame Seed E5 Dog M3 Aspergillus fumigatus F3 Codfish F13 Peanut E3 Horse Dander M4 Mucor racemosus F4 Wheat F17 Hazelnut D201 Blomia tropicalis M5 Candida albicans F5 Rye F18 Brazil Nut G2 Bermuda Grass M6 Alternaria alternata F6 Barley F20 Almond G4 Grass pollen (fescue) M8 Helminthosporium F9 Rice F23 Crab G5 Perennial Rye Grass M9 Fusarium moniliforme F10 Sesame Seed F24 Prawn E82 Rabbit Epithelium M10 Stemphylium botryosum F13 Peanut F27 W1 Common Ragweed M13 Phoma betae F14 Soybean F36 Coconut W9 English Plantain M14 Epicoccum purpurascens F17 Hazelnut F40 Tuna W10 Lamb’s Quarters M15 Trichoderma viride F20 Almond F41 Salmon W21 Parietaria judaica M70 Pityrosporum orbiculare F27 Beef F80 Lobster T18 Eucalyptus D1 D. pteronyssinus F33 Orange F84 Kiwi Fruit T19 Acacia D70 Acarus siro F35 Potato F91 Mango T21 Melaleuca D71 Lepidoglyphus destructor F44 F92 Banana T73 Australian Pine D72 Tyrophagus putrescentiae F45 Yeast F201 Pecan Nut M2 Cladosporium herbarum D73 Glycyphagus domesticus F84 Kiwi Fruit F256 Walnut M3 Aspergillus fumigatus D74 Euroglyphus maynei F91 Mango F290 Oyster M6 Alternaria alternata D201 Blomia tropicalis F92 Banana K82 Latex

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 3 117/03/107/03/10 1:161:16 PMPM CORE INDIVIDUAL ALLERGENS c Core individual allergens, $4 each Note: Requests for more than fi ve of these will exceed our allowance under Medicare

MITES (HOUSE DUST & STORAGE) WEED DRUGS c D1 Dermatophagoides pteronyssinus c W1 Common ragweed c C1 Penicilloyl G c D2 Dermatophagoides farinae c W4 False ragweed c C2 Penicilloyl V c D70 Acarus siro c W5 Wormwood c C5 Ampicilloyl c D71 Lepidoglyphus destructor c W6 Mugwort c C6 Amoxicilloyl c D72 Tyrophagus putrescentiae c W9 English plantain c C7 Cefaclor c D73 Glycyphagus domesticus c W10 Goosefoot (Lamb’s quarters) c C73 Insulin, human c D74 Euroglyphus maynei c W18 Sheep sorrel c C74 Gelatin, bovine c D201 Blomia Tropicalis c W21 Parietaria judaica c H2 House dust VENOMS TREE POLLENS c I1 Honey bee (Apis mellifera) Yellow jacket (Vespula spp. c T3 Birch c I3 Common wasp) c T7 Oak c I4 Paper wasp (Polistes spp.) c T8 Elm Yellow hornet (Dolichovespula c I5 c T9 Olive arenaria) c T11 Sycamore, London plane c T15 White ash c T16 White pine c T17 Japanese cedar c T18 Eucalyptus ANIMAL & AVIAN c T19 Acacia c E1 Cat epithelium & dander c T21 Melaleuca c E3 Horse dander c T23 Italian cypress c E4 Cow dander c T57 Red Cedar c E5 Dog dander c T73 Australian pine c E6 Guinea pig epithelium c T210 Privet pollen INSECTS c E7 Pigeon droppings c I6 Cockroach (Blatella germanica) c E71 Mouse epithelium MOULDS, YEASTS & c I70 Fire ant (Solenopsis invicta) c E73 Rat epithelium c M1 Penicillium notatum c I71 Mosquito spp. (Aedes communis) c E77 Budgerigar droppings c M2 Cladosporium herbarum c I72 Green nimitti (Cladotanytarsus) c E78 Budgerigar feathers c M3 Aspergillus fumigatus c I202 Grain weevil (Sitophilius granarius) c E79 Budgerigar serum proteins c M4 Mucor racemosus c E81 Sheep epithelium c M5 Candida albicans (yeast) c E82 Rabbit epithelium PARASITES c M6 Alternaria alternata c E85 Chicken feathers c P1 Ascaris c M8 Helminthosporium halodes c E201 Canary bird feathers c P4 c M9 Fusarium moniliforme c E213 Parrot feathers c M10 Stemphylium botryosum c E215 Pigeon feathers MISCELLANEOUS c M13 Phoma betae c O1 Cotton crude fi bres c M14 Epicoccum purpurascens c O70 Seminal fl uid GRASS & GRAIN POLLENS c M15 Trichoderma viride c G1 Sweet vernal grass c M70 Pityrosporum orbiculare

c G2 Bermuda grass c M80 Staphylococcus enterotoxin A OCCUPATIONALS c G4 Grass pollen (Fescue) c M202 Cephalosporium acremonium c K75 Isocyanate TDI c G5 Rye-grass c M205 Trichophyton rubrum c K76 Isocyanate MDI c G6 Timothy grass c M227 Malassezia spp. c K77 Isocyanate HDI c G8 Meadow grass c K80 /Formalin c G10 Johnson grass c K82 Latex, Hevea braziliensis c G11 Brome grass c K84 Sunfl ower seed c G15 Cultivated wheat c G17 Paspalum

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 4 117/03/107/03/10 1:161:16 PMPM CORE INDIVIDUAL ALLERGENS c Core individual allergens, $4 each Note: Requests for more than fi ve of these will exceed our allowance under Medicare

FOOD c F40 Tuna c F218 (sweet pepper) c F1 Egg white c F41 Salmon c F220 c F2 Milk c F44 Strawberry c F221 Coffee c F3 Fish (cod) c F45 Yeast (S. cerevisiae) c F222 Tea c F47 Garlic c F224 Poppy seed c F48 Onion c F225 Pumpkin c F49 c F226 Pumpkin seed c F59 Octopus c F235 Lentil c F75 Egg yolk c F237 Apricot c F79 Gluten c F242 Cherry c F80 Lobster c F244 Cucumber c F81 Cheese Cheddar-type c F247 Honey c F82 Cheese Mould-type c F253 Pine nut (pignoles) c F83 Chicken c F255 Plum c F84 Kiwi fruit c F256 Walnut c F4 Wheat c F258 Squid c F5 Rye c F259 Grape c F6 Barley c F260 Broccoli c F7 Oat c F261 Asparagus c F8 Maize (Corn) c F262 Aubergine (eggplant) c F9 Rice c F263 Green pepper (unripe seed) c F10 Sesame seed c F267 Cardamon c F11 c F270 c F12 Pea c F279 c F13 Peanut c F280 Black pepper c F281

c F85 c F284 Turkey meat c F86 Parsley c F287 Red Kidney Bean c F87 Melons c F290 Oyster c F88 Mutton c F291 Caulifl ower c F89 Mustard c F293 c F90 Malt c F294 Passionfruit c F91 Mango fruit c F299 Sweet c F92 Banana c F300 Goat milk c F93 Cacao c F302 Mandarin c F94 Pear c F309 Chickpea c F14 Soya bean c F95 c F315 Green bean c F15 White bean c F96 c F317 Coriander c F17 Hazel nut c F201 Pecan nut c F322 Red currant c F18 Brazil nut c F202 Cashew nut c F324 Hop (fruit cone) c F20 Almond c F203 Pistachio c F329 Watermelon c F23 Crab c F204 Trout c F333 Linseed c F24 Shrimp c F207 Clam c F335 Lupin c F25 c F208 Lemon c F338 Scallop c F26 c F209 Grapefruit c F340 Cochineal (Carmine red, E120) c F27 Beef c F210 c F345 Macadamia nut c F31 Carrot e F211 Blackberry c F346 Abalone c F33 Orange c F212 Mushroom (champignon) c F347 Quinoa c F35 Potato c F214 Spinach c F36 Coconut c F215 Lettuce c F37 Blue mussel c F216 Cabbage

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 5 117/03/107/03/10 1:161:16 PMPM CORE ALLERGEN MIXES c Core mixed allergens $8 Note: Requests for more than two of these will exceed our allowance under Medicare

HOUSE DUST MOULD MIXES c HX2 (H2 D1 D2 I6) Dust & Mite Mix c MX2 (M1 M2 M3 Mould Mix Penicillium M5 M6 M8) chrysogenum, Cladosporium herbarum, Aspergillus fumigatus, ANIMAL MIXES Candida albicans, c EX1 (E1 E3 E4 E5) Animal Mix 1 Cat dander, Horse Alternaria alternata, dander, Cow dander, Setomelanomma Dog dander rostrata c EX2 (E1 E5 E6 Animal Mix 2 Cat dander, Dog dander, E87 E88) Guinea pig epithelium, Rat, Mouse FOOD MIXES c EX71 (E70 E85 E86 Feather Mix Goose feathers, Chicken c FX1 (F13 F17 F18 Nut Mix Peanut, Hazel nut, Brazil E89) feathers, Duck feathers, F20 F36) nut, Almond, Coconut Turkey feathers c FX2 (F3 F24 F37 Seafood Mix Fish, Shrimp, Blue c EX72 (E78 E201 Bird Mix Budgerigar feathers, F40 F41) mussel, Tuna, Salmon E196 E213 Canary bird feathers, c FX3 (F4 F7 F8 F10 Cereal Mix Wheat, Oat, Maize, E214) Parakeet feathers, Parrot F11) Sesame seed, feathers, Finch feathers Buckwheat c FX5 (F1 F2 F3 F4 Staple Food Mix Egg white, Milk, Fish, F13 F14) Wheat, Peanut, Soybean GRASS MIXES c FX15 (F33 F49 F92 Fruit Mix Orange, Apple, Banana, c GX2 (G2 G5 G6 Grass Mix Bermuda grass, Rye F95) Peach G8 G10 G17) grass, Timothy grass, c FX19 (F31 F35 Vegetable Mix Carrot, Potato, Spinach, Meadow grass, Johnson F214 F244) Cucumber grass, Bahia grass c FX70 (F272 F274 Spice Mix 1 Tarragon, Marjoram, F273 F275) Thyme, Lovage c FX71 (F265 RF266 Spice Mix 2 Caraway, Mace, TREE MIXES F267 F268) Cardamom, c TX7 (T9 T12 T16 Tree Mix Olive, Willow, White Pine, c FX72 (F269 F219 Spice Mix 3 Basil, Fennel seed, T18 T19 T21) Eucalyptus, Acacia, F270 F271) Ginger, Melaleuca c FX73 (F26 F27 F83) Meat Mix Pork, Beef, Chicken

WEED MIXES OCCUPATIONAL MIXES c WX1 (W1 W6 W9 Weed Mix Common ragweed, W10 W11) Mugwort, English c PAX5 (K75 K76 Chemicals 1 Isocyanates (TDI, MDI, Plantain, Goosefoot K77 K79) HDI), Phthalic anhydride Lamb’s Quarters, c PAX6 (K78 K79 Chemicals 2 Ethylene oxide, Saltwort K80 K85) Phthalic anhydride, c WX5 (W1 W6 W7 Weed & Flower Common ragweed, Formaldehyde, W8 W12) Mix Mugwort, Ox-eye Daisy, Chloramin T Dandelion, Golden rod

ESOTERIC INDIVIDUAL ALLERGENS e Esoteric individual allergens, $12 each Note: Requests for more than one of these will exceed our allowance under Medicare. Some esoteric allergen results may take up to fi ve weeks

MITES (HOUSE DUST & STORAGE) Mouse epithelium, serum & urine e E88 e E210 Fox epithelium proteins e D3 Dermatophagoides microceras e E211 Rabbit urine proteins e E89 Turkey feathers e E214 Finch feathers e E196 Parakeet feathers e E216 Deer epithelium ANIMAL & AVIAN PROTEINS e E197 Parakeet droppings e E217 Ferret epithelium e E70 Goose feathers e E198 Parakeet serum e E218 Chicken droppings e E72 Mouse urine proteins e E199 Canary bird serum e E219 Chicken serum proteins e E74 Rat urine proteins e E200 Canary bird droppings e E220 Cat serum albumin e E75 Rat serum proteins e E202 Reindeer epithelium e E221 Dog serum albumin e E76 Mouse serum proteins e E203 Mink epithelium e E222 Swine serum albumin e E80 Goat epithelium e E204 BSA (bovine serum albumin) e E83 Swine epithelium e E205 Horse serum proteins e E84 Hamster epithelium e E206 Rabbit serum proteins PARASITES e E86 Duck feathers e E208 Chinchilla epithelium e P2 Echinococcus Rat epithelium, serum & urine e E87 e E209 Gerbil epithelium proteins

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 6 117/03/107/03/10 1:161:16 PMPM ESOTERIC INDIVIDUAL ALLERGENS e Esoteric individual allergens, $12 each Note: Requests for more than one of these will exceed our allowance under Medicare. Some esoteric allergen results may take up to fi ve weeks

MISCELLANEOUS e T212 Cedar e M208 Chaetomium globosum e O201 Tobacco leaf e T213 Pine e M211 Trichophyton ment. var. interdigitale e O202 Artemia salina fi sh feed e T214 Date e M226 Staphylococcus enterotoxin TSST e O203 Tetramin fi sh feed e T217 Peppertree e M223 Staphylococcus enterotoxin C e O207 Daphnia fi sh feed e T218 Virginia live oak e M228 Aspergillus fl avus e O211 Mealworm Tenebrio mollitor e T222 Cypress e O212 Streptavidin e T223 Oil palm OCCUPATIONALS e K70 Green coffee bean GRASS & GRAIN POLLENS WEED & CROP POLLENS e K71 Castor bean e G3 Cocksfoot e W2 Western ragweed e K72 Ispaghula e G7 Common reed e W3 Giant ragweed e K73 Silk waste e G9 Redtop (Bentgrass) e W7 Ox-eye daisy e K74 Silk e G12 Cultivated rye e W8 Dandelion e K78 Ethylene oxide e G13 Velvet grass e W11 Saltwort (Russian thistle) e K79 Phthalic anhydride e G14 Cultivated oat e W12 Golden rod e K81 Ficus spp. e G16 Meadow foxtail e W13 Cocklebur e K83 Cotton seed e G70 Wild rye grass e W14 Common pigweed e K85 Chloramin T e G71 Canary grass e W15 Scale Lenscale e K86 Trimellitic Anhydride (TMA) e G201 Barley e W16 Rough marshelder e K87 Alpha-amylase e G202 Maize (Corn) e W17 Firebush e K201 Papain e G203 Salt grass e W19 Parietaria offi cinalis e K202 Bromelin e G204 False oat-grass e W20 Nettle e K203 Phosphlipase e W22 Japanese Hop e K204 Maxatase e W23 Yellow dock e K205 Alkalase TREE POLLENS e W45 Alfalfa e K206 Savinase e T1 Box-elder e W46 Dog fennel e K208 Lysozyme e T2 Grey alder e W82 Careless weed e K209 Hexahydrophtalic anhydrid e T4 Hazel e W203 Rape (Canola) e K211 MTHP Anhydrid e T5 American beech e W204 Sunfl ower e K212 Abachi wood dust e T6 Mountain juniper e W206 Camomille e K213 Pepsin e T10 Walnut e W207 Lupin e K214 Bougainvillea spp. e T12 Willow e W210 -beet e T14 Cottonwood e T20 Mesquite VENOMS e T22 Pecan Hickory White-faced hornet (Dolichovespula DRUGS e I2 maculata) e T25 European ash e C8 Chlorhexidine European paper wasp (Polistes e I77 e T37 Bald cypress e C70 Insulin porcine dominulus) e T41 White hickory e C71 Insulin bovine e I75 European Hornet (Vespa crabro) e T44 Hackberry e C202 Suxamethonium (Succinylcholine) e I205 Bumblebee e T45 Cedar elm e C208 Tetanus toxoid e T54 Russian olive INSECTS e T55 Scotch broom MOULDS, YEASTS & TOXINS e I8 Moth e T56 Bayberry e M7 Botrytis cinerea e I73 Blood worm e T70 Mulberry e M11 Rhizopus nigricans e I76 Berlin beetle e T71 Red mulberry e M12 Aureobasidium pullulans e I201 Horse bot fl y e T72 Queen Palm e M16 Curvularia lunata e I203 Mediterranean Flour Moth e T201 Spruce e M36 Aspergillus terreus e I204 Horse fl y e T203 Horse chestnut e M81 Staphylococcus enterotoxin B e I206 Cockroach, American e T205 Elder e M201 Tilletia tritici e I207 Cockroach, Oriental e T206 Chestnut e M203 Trichosporon pullulans e T207 Douglas fi r e M204 Ulocladium chartarum e T208 Linden e M207 Aspergillus niger e T209 Horn beam e M209 Penicillium frequentans e T211 Sweet gum

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 7 117/03/107/03/10 1:161:16 PMPM ESOTERIC INDIVIDUAL FOOD ALLERGENS e Esoteric individual food allergens, $12 each Note: Requests for more than one of these will exceed our allowance under Medicare. Some esoteric allergen results may take up to fi ve weeks

FOOD e F272 Tarragon e F320 Crayfi sh e F42 Haddock e F273 Thyme e F321 Horse meat e F50 Chub (mackerel) e F274 Marjoram e F325 Sheep milk e F51 Bamboo shoot e F275 Lovage e F326 Sheep whey e F54 Sweet potato e F276 Fennel fresh e F328 Fig e F55 Common millet e F277 Dill e F330 Rose hip e F56 Foxtail millet e F278 Bay leaf e F331 Saffron e F57 Japanese millet e F283 Oregano e F332 Mint e F58 Pacifi c squid e F285 Elk-Moose meat e F334 Bovine lactoferrin e F60 Jack mackerel (Scad) e F286 Mare’s Milk e F336 Jujube e F61 Sardine (Japanese) Pilchard e F288 Blueberry e F337 Sole e F124 Spelt wheat e F289 Date e F339 e F147 Gulf fl ounder e F292 Guava e F342 Olive (black fresh) e F182 Lima bean e F295 Carambola e F343 Raspberry e F205 Herring e F296 Carob (E410) e F344 Sage e F206 Mackerel e F297 Gum arabic (E414) e F348 Lychee e F213 Rabbit meat e F298 Tragacanth (E413) e F369 Catfi sh e F217 Brussel sprouts e F301 Persimmon e F381 Red Snapper e F219 Fennel seed e F303 Halibut e F384 Whitefi sh e F227 Sugar-beet seed e F304 Langust (spiny lobster) e F410 Grouper e F231 Milk boiled e F305 Fenugreek e F412 Orange roughy e F234 Vanilla e F306 Lime e F413 Pollock e F236 Cow’s whey e F307 Hake e F414 Tilapia e F245 Egg e F308 Sardine(Pilchard) e F415 Walleye pike e F246 Guar, guar gum (E412) e F310 Blue vetch e F254 Plaice e F311 Megrim e F264 Eel e F312 Swordfi sh e F265 Caraway e F313 Anchovy e F266 Mace e F314 Snail e F268 Clove e F316 Rape (Canola) seed e F269 Basil e F318 Jack fruit e F271 Anise e F319 Beetroot

ESOTERIC ALLERGEN MIXES e Esoteric mixed allergens $12 each Note: Requests for more than one of these will exceed our allowance under Medicare. Some esoteric allergen results may take up to fi ve weeks

ANIMAL MIXES e GX4 (G1 G5 G7 Grass Mix 4 Sweet Vernal grass, Rye G12 G13) grass, Common reed, e EX70 (E6 E82 E84 Animal Mix 3 Guinea pig epithelium, Cultivated rye,Velvet E87 E88) Rabbit epithelium, grass Hamster epithelium, Rat, Mouse e GX6 (G2 G5 G10 Grass Mix 6 Bermuda grass, Rye G11 G13 grass, Johnson grass, e EX73 (E70 E85 E86 Animal Mix 4 Goose feathers, Chicken G17) Brome grass, Velvet E213) feathers, Duck feathers, grass, Bahia grass Parrot feathers

COMBINATION INHALANT MIXES GRASS MIXES e RX1 (G6 W6 W9 Inhalant 1 Timothy grass, Mugwort, e GX1 (G3 G4 G5 Grass Mix 1 Cocksfoot, Meadow W21 T3) English Plantain, G6 G8) Fescue, Rye grass, Parietaria, Birch Timothy grass, Meadow grass e RX2 (D2 E1 E3 E5 Inhalant 2 Dermatophagoides M6) farinae, Cat dander, e GX3 (G1 G5 G6 Grass Mix 3 Sweet Vernal grass, Rye Horse dander, Dog G12 G13) grass, Timothy grass, dander, Alternaria Cultivated Rye, Velvet alternata grass

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 8 117/03/107/03/10 1:161:16 PMPM ESOTERIC ALLERGEN MIXES e Esoteric mixed allergens $12 each Note: Requests for more than one of these will exceed our allowance under Medicare. Some esoteric allergen results may take up to fi ve weeks

e RX3 (G2 G5 G17 Inhalant 3 Bermuda grass, Rye MOULD MIXES W1 W9 W10) grass, Bahia grass, e MX1 (M1 M2 M3 Mould Mix 1 Penicillium chrysogenum, Common Ragweed, M6) Cladosporium herbarum, English Plantain, Aspergillus fumigatus, Goosefoot Lamb’s Alternaria alternata Quarters e MX4 (M3 M207 Mould Mix 4 A. fumigatus, A. niger, A. e RX4 (G2 G5 G1 Inhalant 4 Sweet Vernals grass, M36 M228) terreus, A. fl avus W1 W6 W9) Bermuda grass, Rye grass, Common Ragweed, Mugwort, English Plantain, FOOD MIXES e RX5 (D1 E1 M3 I6) Inhalant 5 Dermatophagoides e FX7 (F25 F45 F47 Food Mix 7 Tomato, Yeast, Garlic, pteronyssinus, Cat F48 F85) Onion, Celery dander, Aspergillus e FX8 (F17 F18 F33 Food Mix 8 Hazel nut, Brazil nut, fumigatus, Cockroach F49 F93) Orange, Apple, Cacao e RX6 (T3 G6 W6 Inhalant 6 Birch, Timothy grass, e FX9 (F20 F84 F87 Food Mix 9 Almond, Kiwi, Melons, M2 M6) Mugwort, Cladosporium, F92 F259) Banana, Grape Alternaria alternata e FX10 (F26 F27 F75 Food Mix 10 Pork, Beef, Egg yolk, e RX7 (D1 E1 E3 E5 Inhalant 7 Dermatophagoides F83 F284) Chicken, Turkey E82) pteronyssinus, Cat dander, Horse dander, e FX11 (F8 F12 F15 Food Mix 11 Maize, Pea, White Dog dander, Rabbit F31 F260) bean, Carrot, Broccoli epithelium e FX12 (F5 F9 F35 Food Mix 12 Rye, Rice, Potato, F225 RF212) Mushroom, Pumpkin e FX13 (F12 F15 F31 Food Mix 13 Pea, White bean, TREE MIXES F35) Carrot, Potato e FX14 (F25 F214 F216 Food Mix 14 Tomato, Spinach, e TX1 (T1 T3 T7 T8 Tree Mix 1 Box elder, Birch, Oak, F218) Cabbage, Paprika T10) Elm, Walnut e FX16 (F44 F94 F208 Food Mix 16 Strawberrry, Pear, e TX2 (T1 T7 T8 Tree Mix 2 Box elder, Oak, Elm, F210) Lemon, Pineapple T14 T22) Cottonwood, Pecan Hickory e FX17 (F49 F92 F94 Food Mix 17 Apple, Banana, Pear, F95) Peach e TX3 (T6 T7 T8 Tree Mix 3 Mountain Juniper, Oak, T14 T20) Elm, Cottonwood, e FX18 (F12 F13 F14) Food Mix 18 Pea, Peanut, Soybean Mesquite e FX20 (F4 F5 F6 F9) Food Mix 20 Wheat, Rye, Barley, e TX4 (T7 T8 T11 Tree Mix 4 Oak, Elm, Sycamore, Rice T12 T14) Willow, Cotttonwood e FX21 (F84 F87 F92 Food Mix 21 Kiwi fruit, Melon, e TX5 (T2 T4 T8 Tree Mix 5 Grey Alder, Hazel, Elm, F95 F210) Banana, Peach, T12 T14) Willow, Cottonwood Pineapple e TX6 (T1 T3 T5 T7 Tree Mix 6 Box elder, Birch, e FX22 (F201 F202 Food Mix 22 Pecan nut, Cashew T10) American Beech, Oak, F203 F256) nut, Pistachio, Walnut Walnut e FX23 (F26 F27 F83 Food Mix 23 Pork, Beef, Chicken e TX8 (T1 T3 T4 T7 Tree Mix 8 Box elder, Birch, Hazel, F284) meat, Turkey meat T11) Oak, Sycamore e FX24 (F17 F24 F84 Food Mix 24 Hazel nut, Shrimp, Kiwi e TX9 (T2 T3 T4 T7 Tree Mix 9 Grey Alder, Birch, Hazel, F92) fruit, Banana T12) Oak, Willow e FX25 (F10 F45 F47 Food Mix 25 Sesame seed, Yeast, e TX10 (T2 T3 T4 Tree Mix 10 Grey Alder, Birch,White F85) Garlic, Celery T15) Ash e FX26 (F1 F2 F13 F89) Food Mix 26 Egg white, Cow’s milk, Peanut, Mustard e FX27 (F3 F4 F14 F17) Food Mix 27 Fish (cod), Wheat, WEED MIXES Soybean, Hazel nut e WX2 (W2 W6 W9 Weed Mix 2 Western ragweed, e FX28 (F10 F24 F27 Food Mix 28 Sesame seed, Shrimp, W10 W15) Mugwort, English F84) Beef, Kiwi Plantain, Goosefoot e FX29 (F33 F208 F209 Food Mix 29 Orange, Lemon, Lamb’s Quarters, Scale F302) Grapefruit, Mandarin Lenscale e FX30 (F84 F91 F92 Food Mix 30 Kiwi, Mango, Banana, e WX3 (W6 W9 W10 Weed Mix 3 Mugwort, English F293) Avocado, Papaya W12 W20) Plantain, Goosefoot e FX31 (F49 F94 F95 Food Mix 31 Apple, Pear, Peach, Lamb’s Quarters, Golden F242 F255) Cherry, Plum rod, Nettle e FX32 (F235 F12 F15 Food Mix 32 Lentil, Pea, White e WX6 (W9 W10 Weed Mix 6 English Plantain, RF296) bean, Carob W11 W18) Goosefoot Lamb’s Quarters, Saltwort e FX74 (F3 F205 F206 Food Mix 74 Cod, Herring, Russian Thistle, Sheep F254) Mackerel, Plaice sorrel e WX7 (W7 W8 W9 Weed Mix 7 Ox-eye Daisy, Dandelion, W10 W12) English Plantain, OCCUPATIONAL MIXES Goosefoot Lamb’s e PAX1 (E3 E4 E70 Horse & Cow dander, Quarters, Golden rod E85) Goose & Chicken e WX209 (W1 W2 W3) Weed Mix 209 Common ragweed, feathers Western ragweed, Giant e PAX3 (M3 M6 G12 Aspergillus, Alternaria, ragweed G15) Wheat & Rye pollen e PAX4 (F4 F14 K87 Wheat & Soy fl our, I202) alpha-amylase, Sitophilus granarius

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 9 117/03/107/03/10 1:161:16 PMPM RECOMBINANT AND NATIVE INDIVIDUAL ALLERGEN MOLECULES n Individual Native (n) $40 each r Recombinant (r) allergen molecules $40 each

ANIMALS n F334 nBos d Lactoferrin, Milk Bos spp. MOULDS r E94 rFel d1 (recombinant cat) rPen a 1 Tropomyosin, Shrimp r M218 rAsp f1 (recombinant aspergillus) r F351 Penaeus aztecus nFel d 2 Cat serum albumin Felis r E220 r M219 rAsp f2 (recombinant aspergillus) domesticus r F353 rGly m 4 PR-10, Soy Glycine r M220 rAsp f 3 (recombinant aspergillus) r E101 rCan f1 (recombinant dog) rBer e 1 Brazil nut, Bertholletia r F354 excelsa r M221 rAsp f4 (recombinant aspergillus) r E102 rCan f2 (recombinant dog) r F355 rCyp c 1 Carp Cyprinus carpio r M222 rAsp f6 (recombinant aspergillus) nCan f 3 Dog serum albumin n E221 rApi g 1.01 PR-10, Celery Apium r M229 Canis familiaris r F417 rAlt a 1 (component alternaria) graveolens n E204 nBos d 6 BSA, Cow Bos spp. r F418 rApi g 1.02 (recombinant Celery) LATEX nSus s Pig serum albumin, Swine n E222 rPru p 1 PR-10, Peach Prunus Sus scrofa r F419 r K215 rHev b 1, Latex Hevea brasiliensis persica r K217 rHev b 3, Latex Hevea brasiliensis rPru p 3 LTP, Peach Prunus GRASSES r F420 persica r K218 rHev b 5, Latex Hevea brasiliensis r G205 rPhl p 1 (recombinant timothy) rPru p 4 Profi lin, Peach Prunus rHev b 6.01, Latex Hevea r F421 r K219 r G206 rPhl p 2 (recombinant timothy) persica brasiliensis rTri a 19 Omega-5 Gliadin, Wheat rHev b 6.02, Latex Hevea r G208 rPhl p 4 (native timothy) r F416 r K220 Triticum spp brasiliensis r G209 rPhl p 6 (recombinant timothy) rHev b 8, Profi lin, Latex Hevea r F422 rAra h 1 Peanut Arachis hypogaea r K221 r G210 rPhl p 7 (recombinant timothy) brasiliensis r F423 rAra h 2 Peanut Arachis hypogaea r G211 rPhl p 11 (recombinant timothy) r K222 rHev b 9, Latex Hevea brasiliensis r F424 rAra h 3 Peanut Arachis hypogaea rHev b 11, Latex Hevea r G212 rPhl p 12 (recombinant timothy) r K224 rAra h 8 PR-10, Peanut Arachis brasiliensis rPhl p1; rPhl p5b (recombinant r F352 r G213 hypogaea timothy) rAra h 9 LTP, Peanut Arachis VENOM rPhl p7;rPhl p12 (recombinant r F427 r G214 hypogaea timothy) nApi m 1, Phospholipase A2, Bee rCor a 8 LTP, Hazel nut Corylus n K203 r F425 Apis mellifera r G215 rPhl p 5b (recombinant timothy) avellana nCyn d 1 Bermuda grass, n G216 r F426 rGad c 1 Cod Gadus morhua OCCUPATIONAL ALLERGENS Cynodon dactylon rCor a 1 PR-10, Hazel nut Corylus r F428 n K205 Alkalase, Bacillus spp. avellana FOODS nAsp o 1, a-amylase Aspergillus n K87 oryzae nBos d 4 a-lactalbumin, Milk Bos TREES c F76 nCar p 1, Papain, Papaya Carica spp. n K201 rBet v 1 PR-10, Birch Betula papaya nBos d 5 b-lactoglobulin, Milk r T215 c F77 verrucosa Bos spp. nGal d 4, Lysozyme, Egg Gallus rBet v 2 Profi lin, Birch Betula n K208 r T216 spp. c F78 nBos d 8 Casein, Milk Bos spp. verrucosa n K204 Maxatase, Bacillus licheniformis r T220 rBet v 4, Birch Betula verrucosa c Core individual allergens, $4 each n K206 Savinase, Bacillus spp. rBet v 2, rBet v 4 Birch Betula r T221 Note: These allergens are priced as core verrucosa n K213 nSus s Pepsin, Swine Sus scrofa individual allergens ($4 ea) r T225 rBet v 6 Birch Betula verrucosa OTHERS (to exclude CCD reactivity) FOODS n T224 nOle e 1 Olive Olea europaea n Ro214 MUXF3 CCD, Bromelin nGal d 2 Ovalbumin, Egg Gallus n F232 WEEDS nAna c 2 Bromelin, Pineapple spp. n K202 Ananas comosus nGal d 1 Ovomucoid, Egg Gallus n F233 r W211 rPar j 2 (recombinant parietaria) spp. nAmb a 1, Ragweed Ambrosia nGal d 3 Conalbumin, Egg Gallus n W230 n F323 elatior spp. nArt v 1, Mugwort Artemisia n W231 vulgaris

ImmunoCAP ISAC® ($350 per profile) The ISAC (Immuno Solid-phase Allergen Chip) is a microarray system developed by VBC Genomics and Phadia. ISAC can now allow laboratories to reliably and simultaneously determine specifi c IgE levels to a wide range of clinically important individual allergen molecules more economically than using many individual recombinant CAP allergens. Clinical studies have shown that, for example, even modest levels of serum specifi c IgE level to ara h 2, one of the nine major peanut allergens, are strongly predictive of more severe reactions and poor tolerance of oral challenges. Similarly, modest levels of serum specifi c IgE to ovomucoid, an egg allergen which is heat resistant, are strongly predictive of poor tolerance to even cooked egg in a baked cake; the fi rst challenge usually used by allergy clinics when one is confi dent that a child might be starting to lose their . The ISAC profi les are performed in batches, and the results are reported semi-quantitatively and organised into both the individual allergen molecule source, as well as the protein group from which it comes. These protein groups have particular patterns of clinical signifi cance. For example, specifi c IgE to Transfer Proteins are often associated with systemic and more severe reactions in addition to oral allergy symptoms and these molecules are usually not altered with food preparation and cooking ( digestion and heat). Similarly, seed storage proteins are also heat resistant and often associated with signifi cant symptoms with cooked foods. In contrast, PR-10 proteins are heat labile and are associated with oral allergy symptoms with the raw, rather than cooked foods. Similarly, heat sensitive profi lin proteins are more associated with nuisance rather than severe symptoms. Tropomyosin proteins account for dustmite-crustacean cross-reactivity. Animal albumins and fi sh parvalbumins are important causes of other food reactions. A table of the allergens included in the ISAC profi le is shown opposite.

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 1010 117/03/107/03/10 1:161:16 PMPM ImmunoCAP ISAC® Allergen Components ImmunoCAP ISAC® Allergen Components Allergen Allergen Allergen Source Protein Group Allergen Source Protein Group Component Component COMMON COMMON LATIN NAME LATIN NAME NAME NAME PLANTS NON-PLANTS nCyn d 1 Bermuda grass Cynodon dactylon Grass group 1 nBos d 6 BSA Bos domesticus Serum albumin rPhl p 1 Timothy Phleum pratense Grass group 1 nBos d 8 Cow’s milk Bos domesticus Caseins rPhl p 2 Timothy Phleum pratense Grass group 2 nBos d lactoferrin Bos domesticus Laktoferrin nPhl p 4 Timothy Phleum pratense nGal d 1 Egg Gallus domesticus Ovomucoid rPhl p 5 Timothy Phleum pratense Grass group 5 nGal d 2 Egg Gallus domesticus Ovalbumin rPhl p 6 Timothy Phleum pratense nGal d 3 Egg Gallus domesticus Conalbumin rPhl p 11 Timothy Phleum pratense nGal d 5 CSA (Livetin) Gallus domesticus Serum albumin rBet v 1 Birch Betula verrucosa PR-10 protein rCyp c 1 Carp Cyprinus carpio Parvalbumin rAln g 1 Alder Alnus glutinosa PR-10 protein rGad c 1 Cod Gadus callarias Parvalbumin House dust Dermatophagoides rCor a 1.0101 Hazel pollen Corylus avellana PR-10 protein rDer f 1 mite farinae nCry j 1 Japanese ceder Cryptomeria japonica House dust Dermatophagoides rDer f 2 mite farinae nCup a 1 Cypress Cupressus arizonica House dust Dermatophagoides nDer p 1 nOle e 1 Olive Olea europaea mite pteronyssinus House dust Dermatophagoides nDer p 2 rPla a 1 Plane tree Platanus acerifolia mite pteronyssinus nPla a 2 Plane tree Platanus acerifolia rEur m 2 Storage mite Euroglyphus maynei nAmb a 1 Ragweed Ambrosia artemisiifolia rCan f 1 Dog Canis familiaris Lipocalin nArt v 1 Mugwort Artemisia vulgaris rCan f 2 Dog Canis familiaris Lipocalin nArt v 3 Mugwort Artemisia vulgaris Lipid transfer protein (nsLTP) nCan f 3 Dog Canis familiaris Serum albumin rPar j 2 Wall pellitory Parietaria Judaica Lipid transfer protein (nsLTP) nEqu c 3 Horse Equus caballus Serum albumin nSal k 1 Saltwort Salsola kali rFel d 1 Cat Felis domesticus Uteroglobin nAct d 1 Kiwi Actinidia deliciosa nFel d 2 Cat Felis domesticus Serum albumin nAct d 2 Kiwi Actinidia deliciosa nMus m 1 Mouse Mus musculus Lipocalin nAct d 5 Kiwi Actinidia deliciosa rAlt a 1 Alternaria Alternaria alternata nAct d 8 Kiwi Actinidia deliciosa PR-10 protein rAlt a 6 Alternaria Alternaria alternata rApi g 1 Celery Apium graveolens PR-10 protein rAsp f 1 Aspergillus Aspergillus fumigatus rDau c 1 Carrot Daucus carota PR-10 protein rAsp f 2 Aspergillus Aspergillus fumigatus rMal d 1 Apple Malus domestica PR-10 protein rAsp f 3 Aspergillus Aspergillus fumigatus rPru p 1 Peach Prunus persica PR-10 protein rAsp f 4 Aspergillus Aspergillus fumigatus nPru p 3 Peach Prunus persica Lipid transfer protein (nsLTP) rAsp f 6 Aspergillus Aspergillus fumigatus Cladosporium rAna o 2 Cashew nut Anacardium occidentale rCla h 8 Cladosporium herbarum nAra h 1 Peanut Arachis hypogaea Storage protein, vicilin Honey bee nApi m 1 Apis mellifera Phospholipase A2 nAra h 2 Peanut Arachis hypogaea Storage protein, Conglutin venom Honey bee nApi m 4 Apis mellifera Melittin nAra h 3 Peanut Arachis hypogaea Storage protein, 11S globulin venom rAra h 8 Peanut Arachis hypogaea PR-10 protein rBla g 1 Cockroach Blattella germanica rBer e 1 Brazil nut Bertholletia excelsa Storage protein, 2S albumin rBla g 2 Cockroach Blattella germanica rCor a 1.0401 Hazelnut Corylus avellana PR-10 protein rBla g 4 Cockroach Blattella germanica rCor a 8 Hazelnut Corylus avellana Lipid transfer protein (nsLTP) rBla g 5 Cockroach Blattella germanica nCor a 9 Hazelnut Corylus avellana Storage protein, 11S globulin rAni s 1 Anisakis Anisakis simplex rGly m 4 Soybean Glycine max PR-10 protein CROSS REACTIVE MARKERS, NON-PLANTS nGly m 5 Soybean Glycine max Storage protein, b-conglycinin rAni s 3 Anisakis Anisakis simplex Tropomyosin nGly m 6 Soybean Glycine max Storage protein, glycinin nBla g 7 Cockroach Blattella germanica Tropomyosin nSes i 1 Sesame seed Sesamum indicum Storage protein, 2S albumin House dust Dermatophagoides rDer p 10 Tropomyosin nTri a 18 Wheat Triticum aestivum mite pteronyssinus nTri a gliadin Wheat Triticum aestivum Crude gliadin rPen a 1 Shrimp Penaeus aztecus Tropomyosin rTri a 19.0101 Wheat Triticum aestivum Omega-5 gliadin nPen i 1 Shrimp Penaeus indicus Tropomyosin nTri a aA_TI Wheat Triticum aestivum nPen m 1 Shrimp Penaeus monodon Tropomyosin rHev b 1 Latex Hevea brasiliensis rHev b 3 Latex Hevea brasiliensis rHev b 5 Latex Hevea brasiliensis ISAC rHev b 6 Latex Hevea brasiliensis ology FEBRUARY 2010

Individual Recombinant/ CROSS REACTIVE MARKERS, PLANTS CONTENTS Purified Allergen Individual 1-2 Recombinant/ Purified Allergen Molecules and Allergen Calcium binding protein, Molecules and Allergen rBet v 4 Birch Betula verrucosa Microarray testing Microarray testing

Polcalcin ImmunoCAP ISAC® 2-3 Previously on skin test systems The ability to identify which Component- 4-5 or laboratory test systems, we molecules are involved in allergic Calcium binding protein, Resolved could only measure specic IgE sensitisation is referred to as Diagnostics to an allergen source, usually component-resolved diagnosis. rPhl p 7 Timothy Phleum pratense Classes of Proteins a mixture of many different The Phadia ISAC ImmunoCAP Involved in Allergy molecules. In the most important system presently proles specic Polcalcin practical advance in laboratory IgE to 103 individual allergen Practical Clinical 6-8 allergy diagnostics, it is now molecules from 47 different Applications possible to determine the amount allergens, while separate of ISAC and of circulating specic IgE to ImmunoCAP reagents are rBet v 2 Birch Betula verrucosa Profilin Recombinant/ individual allergen molecules. available to test one molecule Native Individual This can be performed for a at a time when the question is Allergen Molecule Tests unique molecule of interest more selected. Unfortunately, the using separate ImmunoCAPs Medicare Benets Schedule has Indications for 8 or, for multiple molecules, on a long neglected reimbursement rHev b 8 Latex Hevea brasiliensis Profilin ISAC Profiles microarray platform called the levels for in vitro specic IgE ISAC ImmunoCAP. Work began testing and the current rebate Indications 9 on isolating individual allergen ($22.95) for an episode of for selected molecules of interest in the laboratory allergy testing is individual/ late 1970s, and on developing considerably less than the reagent rMer a 1 Annual mercury Mercurialis annua Profilin recombinant microarray platforms in 2003. cost of these test systems. Our individual allergen Early basic, and later clinical, laboratory charges $40 per molecule tests research has resulted in mature recombinant or native individual technologies available to allergen molecules, with the Suspicion of 10 clinicians through specialised exception of three puried milk nOle e 2 Olive Olea europaea Profilin Suspicion of 11 laboratories. Since IgE reactivity molecules, and $350 for the ISAC Egg allergy to some individual molecules prole of 103 individual allergens can result in potentially serious on a microarray. Since ISAC clinical events, these test systems is a much more cost-efcient can provide new diagnostic approach to such testing, I have and prognostic information called this newsletter ‘ISACology’ rPhl p 12 Timothy Phleum pratense Profilin to clinicians looking after and will provide progressive allergic patients. updates with clinical vignettes. nAna c 2 Bromelain Ananas comosus CCD marker

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 1111 117/03/107/03/10 1:161:16 PMPM BILLING POLICY FOR ALLERGY TESTING

Our policy is to respect your request for the allergens and decode them according to our best practice. If you have requested more allergens than our allowance under Medicare, we will bill your patient according to the pricing in this menu. If you have nominated the patient to be bulk-billed or if the patient has signed a Medicare assignment form, this billing policy will still apply for all tests above our allowance under Medicare. You will see in our Allergy menu that we now list a price for each allergen reagent and that this is organised into different allergen classes. Core individual allergens are $4, core mixed allergens are $8, esoteric individual allergens are $12 and esoteric mixed allergens are also $12. Our highly specialised recombinant (r) or native (n) individual allergen molecules are $40, and the ISAC profi le, which tests 103 different individual allergen molecules from 47 different allergens, is $350. If the price of the allergens requested is more than the Medicare rebate of $22.95, the laboratory will not accept bulk-billing for the request and the patient will be billed according to the price on the menu and may claim a rebate, subject to coning. Practically, this means we will not accept a bulk-billed request for more than fi ve core individual allergens, or two core mixed allergens, or more than one esoteric individual or mixed allergens. However, one core mixed allergen plus three core individual allergens would be covered by the Medicare rebate. Similarly, two core mixed allergens plus one core individual allergen would be covered under our allowance under Medicare. One esoteric mixed or individual allergen plus two core individual allergens or one core mixed allergen would be covered under our allowance under Medicare. A simple table summarises this for you and your patients. Please note that eligible patients may be able to claim a rebate on four occasions per year for testing performed on separately collected blood, with each episode at least 14 days apart.

WHAT CAN YOU ORDER THAT IS MEDICARE REBATABLE?

Our Fee For Different Allergen Types Core individual $4 Core mix $8 Esoteric Individual $12 Esoteric Mix $12 Recombinant/ Native $40 ISAC Profi le $350

Medicare Rebatable Private Bill

Combinations beyond the black line will be privately billed but patients may be eligible for a Medicare rebate of $22.95.

As the list of allergens is constantly being expanded, you may wish to enquire about the availability of a particular allergen. Please contact Customer Service on 4224 7400 or for additional advice, Michael Fox (Special Services Manager) on 4224 7366. re hu h oc o r B Br 0 0-B 0 1 0 01 - -20 - 2 2 V A AV2A -A - S S &S SOUTHERN IML PATHOLOGY • ABN 45 DENISON STREET • WOLLONGONG • NSW 2500 & B& B /B /

A subsidiary of SONIC HEALTHCARE TEL (02) 4224 7474 • FAX: (02) 4224 7444 P

MAIL ADDRESS • LOCKED BAG 35 • WOLLONGONG • NSW 2500 MP M HM H DHM D

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aallergy(2010)_southIML.inddllergy(2010)_southIML.indd 1212 117/03/107/03/10 1:161:16 PMPM