AP Unit 5 Agenda 12-13

AP Biology

Belton High School



Unit Five Agenda—DNA

Day Topic Objectives

11/4-11/5 Central Dogma 1-2

History of DNA

11/6 Structure of DNA & RNA 3-6

Prokaryotic vs. Eukaryotic Genomes

11/7 Replication 7-8

Meselson and Stahl Case Study

11/10 Restriction Enzymes 9-11


11/11 Lab: Electrophoresis

11/12 Lab: Electrophoresis

11/13 Wrap Review

11/14 Unit Test

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Unit Objectives: DNA

At the end of this unit you should be able to:

1.  Describe what is meant by the “central dogma.”

2.  Describe the work of each of the following sets of people in the discovery of DNA: Watson & Crick, Wilkins & Franklin, Hershey & Chase, Avery, MacLeod & McCarty.

3.  Compare the structure and size of eukaryotic and prokaryotic genomes including: number and shape of “chromosomes,” cellular location, and presence of plasmids.

4.  List the components of DNA and RNA nucleotides and identify important structural differences between DNA and RNA (include type of sugar, number of strands and identity of nitrogenous bases).

5.  Distinguish between the structures of purines and pyrimidines and identify which of the nitrogenous bases found in DNA and RNA belong to each group.

6.  Describe the antiparallel structure of a DNA molecule including: the difference between the 3’ and 5’ ends, the pairing of bases, the chemical bond connecting nucleotides of each strand and the chemical bond holding the two strands together.

7.  Explain what it means to say that DNA replication is a semiconservative process.

8.  Describe the process of DNA replication (including the differential formation of the leading and lagging strands) and explain the roles of helicase, topoisomerase, DNA polymerase and ligase in the process.

9.  Describe the natural role of restriction enzymes and explain HOW they act to cut DNA.

10.  Describe the process of gel electrophoresis and give examples of real world applications of this technology.

11.  Explain how restriction enzymes and gel electrophoresis are used to isolate and order DNA fragments to allow for restriction fragment analysis (to create a DNA fingerprint).