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Validation, Quality Assessment and Quality Control of Diagnostic Assays and Vaccine for Vesicular Diseases in Europe
OIE Collaborating Centre Reports
Activities in 2013
Title of Collaborating Centre: / Validation, Quality Assessment and Quality Control of Diagnostic Assays and Vaccine Testing for Vesicular Diseases in EuropeAddress of Collaborating Centre: / Veterinary and Agrochemical Research Centre
(CODA-CERVA)
Groeselenberg 99, B-1180 Ukkel
BELGIUM
Tel.: / +3223790400
Fax: / +3223790666
e-mail address: /
website: / http://www.coda-cerva.be
Name of Director of Institute (Responsible Official): / Dr Pierre Kerkhofs
Name (including Title and Position) of Head of the Collaborating Centre (formally OIE Contact Point): / Dr Kris De Clercq
Head of Unit
Name (including Title and Position) of writer of this report
(if different from above) / Dr. Kris De Clercq
Head of Unit
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories
ToR: To identify and maintain existing expertise, in particular within its region
1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE
Disease control
Epidemiology, surveillance, risk assessment, modelling
Training, capacity building
Zoonoses
Wildlife
Avian diseases
Aquatic animal diseases
Animal welfare
Diagnosis, biotechnology and laboratory
Veterinary medicinal products
Vaccines
Food safety
Feed safety
Other (Name the category: )
DIAGNOSIS, BIOTECHNOLOGY AND LABORATORYTitle of activity / Scope
Evaluation of the quality of NSP-ELISAs with samples from animals infected/vaccinated with foot-and-mouth disease virus (FMDV) strains belonging to 4 serotypes / A total of 7 new NSP-ELISAs, commercially available or in development by laboratories or companies were evaluated with two serum panels (as described by the OIE FMD Ref Centre Panaftosa, Brasil, in the article of Campos et al., 2008, and by the OIE FMD Ref Centre at Pirbright, UK, in the article of Parida et al., 2007). This was done in full collaboration between the OIE CC Coda-Cerva, Ukkel-Belgium, and the OIE Reference Centre for FMD in Brescia, Italy.
Evaluation of the quality of serological assays against structural proteins of FMDV / The quality of the SP-ELISA and virus neutralisation test for the detection of antibodies against FMDV were evaluated by an interlaboratory comparision using field samples (N = 506). This was done in full collaboration between the OIE CC Coda-Cerva, Ukkel-Belgium, and the OIE Reference Centre for FMD in Brescia, Italy.
Development of diagostic serological kits for FMDV / To harmonise the quality and the outcome of serological tests to detect antibodies against FMDV and therefore the controle of FMD, post vaccination sera from cattle against the 7 serotypes of FMDV and taken 21 days post vaccination and 10 days post revaccination were provided in an amount of 50 ml each to a diagnostic kit producer in France for the development of serological kits in collaboration with CODA-CERVA as OIE CC and ANSES as National FMD Reference Centre for France.
Other:
Title of activity / Scope
Category: Vaccines.
Evaluation of the use of RT-PCRs for quality control testing of vaccines. / The blood of 100 cattle was checked after vaccination by real-time RT-PCR. The test detected the presence of vaccinal RNA. This might interfere with the determination of a viremia after infection and thus with the evaluation of the protective power of the vaccine. This vaccine RNA carriage appears to be short lasting. Therefore paired sampling procedures should be implemented to allow the discrimination between infectious virus and vaccine-derived RNA avoiding any misinterpretation.
ToR: To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty
2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare
Proposal title / Scope/Content / Applicable areaThe implementation of parallel testing using multiple real-time PCRs with similar characteristics to improve diagnosis in disease control or eradication programmes. / This was done in full collaboration between the OIE CC Coda-Cerva, Belgium, and the Laboratoire de Virologie et Hygiène & Microbiologie, Faculté des Sciences et Techniques, Mohammedia, Morocco and the Laboratoire Régional d’Analyses et de Recherches d’Oujda, Office National de Sécurité Sanitaire des Produits Alimentaires, Oujda, Morocco. / Surveillance and control of animal diseases
Food safety
Animal welfare
ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations
3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies?
Yes No
If the answer is yes, please provide details using the following table
Name of OIE CC/RL/other organisation(s) / Location / Region of networking Centre / Purposethe OIE FMD Reference Laboratory ARRIAH of the Russian Federation,
the OIE FMD Reference Laboratory Pirbright Laboratory UK,
the OIE CC Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI) Germany,
the OIE FMD Reference Laboratory OVI, Onderstepoort, South-Africa,
the OIE FMD Reference Laboratory Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Brescia, Italy
the OIE FMD Reference Laboratory SENASA, Buenos Aires, Argentina
the OIE FMD Reference Laboratory BVI, Gaborone, Botswana
the OIE FMD Reference Laboratory PANAFTOSA, Brasil
the OIE FMD Reference Laboratory LVRI, Lanzou, China
the OIE FMD Reference Laboratory PIADC, Plum Island, US
the National FMD Reference Laboratory ANSES, France / the Russian Federation,
UK,
German
South-Africa,
Italy
Argentina
Botswana
Brasil
China
US
France / Africa
Americas
Asia and Pacific
Europe
Middle East / OIE/FAO FMD Reference Laboratory Network
Vaccine matching
International proficiency tests
Post vaccination monitoring
FMD virus and sequence exchange
4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies?
Yes No
If the answer is yes, please provide details using the following table
Name of OIE CC/RL/other organisation(s) / Location / Region of networking Centre / Purposethe OIE Bluetongue Reference Laboratory, Pirbright Laboratory, UK
the OIE Poxvirus Reference Laboratory, Pirbright Laboratory, UK / UK / Africa
Americas
Asia and Pacific
Europe
Middle East / Quality control of differential diagnostic assays
ToR: To place expert consultants at the disposal of the OIE.
5. Did your Collaborating Centre place expert consultants at the disposal of the OIE?
Yes No
Name of expert / Kind of consultancy / SubjectDr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq
Dr Kris De Clercq / Vice-President
Participant
Mission Team Member
Member Expert Group
Vice-President
Representing SCAD
Mission Team Member
Representing SCAD / Meeting SCAD, Paris, 4-8/ 02/2013
OIE General Session, Paris, 27-30/ 05/2013
OIE Mission Zonal FMD Status, Thailand, 15-22/ 06/2013
OIE-FAO FMD Post vaccination monitoring guidelines, Rome, 27-28/ 06/2013
Meeting SCAD, Paris, 2-6/ 09/2013
OIE FMD ad hoc group meeting, Paris, 21-24/ 10/2013
OIE Mission Zonal FMD Status, Namibië, Botswana, Swaziland, Zuid-Afrika, 26/10 – 9/11/2013
OIE FMD ad hoc group meeting, Paris, 25-29/ 11/2013
Dr Ann Brigitte Cay
Ir Tom Willems
Dr Ann Brigitte Cay
Dr David Lefebvre / Member AHG
Member GFRA
Member AHG
Participant / Meeting AHG Schmallenberg virus, Paris, 7– 8/05/2013
Global Foot-and-Mouth Disease Research Alliance (GFRA), Tanzania, 09-10/ 10/2013
Meeting AHG Schmallenberg virus, Paris, 10 – 11/10/2013
9th OIE/FAO FMD Reference Laboratories Network Annual Meeting, Bangkok, Thailand, 14-15/11/2013
ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries
6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries?
Yes No
If the answer is yes, please provide the total number of trained persons for each of the following categories:
a) Technical visits:
b) Seminars: 4
c) Hands-on training courses:
d) Internships (>1 month):
Please provide details (specify the type of training provided using the letter ranked above/overleaf)
Type of technical training provided (a, b, c or d) / Content / Country of origin of the expert(s) provided with training / No. participants from the corresponding countryb / Quality assurance for packaging of vesicular samples according to the International biosafety rules and IATA norms / Nigeria / 2
b / Quality assurance for packaging of vesicular samples according to the International biosafety rules and IATA norms / Botswana / 2
ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE
7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE?
Yes No
National/International / Title of event / Co-organiser / Date (mm/yy) / Location / No. Participants
International / OIE-FAO FMD Post vaccination monitoring guidelines / OIE, FAO / June 2013 / Rome / 20
ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty
8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 5
Haegeman A, Zro K, Vandenbussche F, Demeestere L, Van Campe W, Ennaji MM, De Clercq K (2013). Development and validation of three Capripoxvirus real-time PCRs for parallel testing. J. Vir. Methods, 193(2):446-451.Welby S, Méroc E, Faes C, De Clercq K, Hooyberghs J, Mintiens K, Van der Stede Y (2013). Bluetongue surveillance system in Belgium: a stochastic evaluation of its risk based approach effectiveness. Prev Vet Med. 112(1-2):48-57.
Celma CC, Boyce M, van Rijn PA, Eschbaumer M, Wernike K, Hoffmann B, Beer M, Haegeman A, De Clercq K, Roy P. (2013). Rapid Generation of Replication-Deficient Monovalent and Multivalent Vaccines for Bluetongue Virus: Protection against Virulent Virus Challenge in Cattle and Sheep. J Virol. 87(17):9856-64.
De Leeuw I, Garigliany M, Bertels G, Willems T, Desmecht D, De Clercq K (online ahead of publication). Bluetongue virus RNA detection by real-time RT-PCR in post-vaccination samples from cattle. Transbound Emerg Dis. 2013 Apr 24. doi: 10.1111/tbed.12100. [Epub ahead of print], PMID:23611408
Lefebvre DJ, De Vleeschauwer AR, Goris N, Kollanur D, Billiet A, Murao L, Neys J., De Clercq K (On-line). Proof-of-concept for the inhibition of foot-and-mouth disease virus replication by the antiviral drug 2’-C-methylcytidine in severe combined immunodeficient mice. Transbound Emerg Dis. 2013 Mar 11. doi: 10.1111/tbed.12069. [Epub ahead of print], PMID:23480064
b) International conference presentations:
c) National conference presentations:
d) Other:
(Provide website address or link to appropriate information)
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Annual reports of OIE Reference Centres, 2013 7