DOCSLIB.ORG

Umbilical Cord Blood Platelet Rich Plasma Derivatives for Therapeutic Applications and Development of Clinical Trials for Treatment of Skin and Ocular Ulcers

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Umbilical Cord Blood Platelet Rich Plasma Derivatives for Therapeutic Applications and Development of Clinical Trials for Treatment of Skin and Ocular Ulcers ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi queda condicionat a lʼacceptació de les condicions dʼús establertes per la següent llicència Creative Commons: http://cat.creativecommons.org/?page_id=184 ADVERTENCIA. El acceso a los contenidos de esta tesis queda condicionado a la aceptación de las condiciones de uso establecidas por la siguiente licencia Creative Commons: http://es.creativecommons.org/blog/licencias/ WARNING. The access to the contents of this doctoral thesis it is limited to the acceptance of the use conditions set by the following Creative Commons license: https://creativecommons.org/licenses/?lang=en PhD THESIS Title: Umbilical Cord Blood Platelet Rich Plasma derivatives for therapeutic applications and development of clinical trials for treatment of skin and ocular ulcers PhD Student: Dinara Samarkanova Directors: Dr. Sergio Luis Querol Giner Dr. Ricardo Pedro Casaroli-Marano Tutor: Dr. Susan Webb Youdale Department: PhD in Medicine, Department of Medicine, Universitat Autònoma de Barcelona Barcelona, 2020 The future belongs to those who believes in the beauty of their dreams. Eleanor Roosevelt 2 Acknowledgements: First of all, I would like to acknowledge Dr Sergio Querol. Thank you for showing me how to stand my ground and have a career that I can be proud of. I can count myself lucky for having had you as my mentor. Accept my heartfelt gratitude for your time, patience, support, and trust. Thank to Dr Ricardo Casaroli for support and to be a part of this work. I really appreciate your advice and support. Also for kind tutorship of Dr. Susan Webb. I thank cord blood donors and health care professionals in all hospitals participating in the Programa Concordia BST for making the public cord blood program possible. I thank the Banc de Sang i Teixits staff, including central and territorial centers, for all support and kind help during the projects, and all colleagues in the Cell Therapy service for their incredible support, especially to Dr Luciano Rodriguez for his support and improving the quality of projects, as well as for facilitating me the attendance of patients. My gratitude to Professor Paolo Rebulla for mentorship, his support, scientific consultations during the progression of the projects, and critical revision of manuscripts. I would like thank all Hospital staff who took a part in the projects development and took care of each patient treated with our products. Additionally, I acknowledge to Anthony Nolan Research Institute staff, especially Professor Alejandro Madrigal, Dr. Diana Hernandez and Dr. Steven Cox. I also would like thank Dr. Margarita Codinach, Dr. Emma Enrich and Dr. Anna Belen Alvarez for their critical revision of the Thesis. The biggest acknowledgment is for my loving mother – Samarkanova Sholpan, very positive person, she always insisted on investing in education and never give up. During the PhD I also built a family and had my best project – my son – Dani – the fruit of globalization. Thanks to my husband – Leonardo Colussi for his love and support. Special mention to all those who have accompanied me on this interesting path to pursue the development of this PhD thesis. 3 Abbreviations: BST – Banc de Sang i Teixits CT – clinical trial CB – cord blood CBB – cord blood bank CBED – cord blood eye drops CBPG – cord blood platelet gel GVHD – graft versus host disease PB – peripheral blood PBMC – peripheral blood mononuclear cells PC – platelet concentrates PG – platelet gel PL – platelet lysate PPP – platelet poor plasma PR – platelet releasate PRP – platelet rich plasma 4 Index: Abstract ................................................................................................................................................. 7 Resumen ................................................................................................................................................ 8 I. Introduction: .................................................................................................................................. 9 1. Cord blood for therapy: state-of-the art ................................................................................. 10 1.1. Cord blood for transplantation ........................................................................................ 10 1.2. Cord blood Banks ............................................................................................................ 10 1.3. Cord blood collection and processing ............................................................................. 11 1.4. Cord blood beyond transplantation ................................................................................ 12 2. Cord blood derived medicinal products ............................................................................... 13 2.1. Cord blood as starting material for advanced therapies................................................. 15 2.2. Multicomponent cord blood bank. ................................................................................. 16 3. Plasma, platelets and other bioactive components in cord blood ............................................ 17 4. The wound healing .................................................................................................................. 22 5. Cord blood platelet rich plasma derivatives in wound healing ............................................... 22 5.1. Mechanism of action ....................................................................................................... 22 5.2. Clinical uses ..................................................................................................................... 24 5.2.1. PRP derived product for skin lesions ........................................................................... 24 5.2.2. Blood derived eye drops for ophthalmic application .................................................. 27 5.3. Other application of cord blood PRP derivatives ............................................................ 32 II. Hypothesis ................................................................................................................................... 34 III. Objectives ................................................................................................................................ 36 IV. Materials & Methods .............................................................................................................. 38 Paper 1. Cord blood derived platelet concentrates as starting material for new therapeutic blood- components prepared in a public cord blood bank: from product development to clinical application....................................................................................................................................... 40 Paper 2. Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine .......................................................................................................................................... 53 Paper 3. Clinical evaluation of allogeneic eye drops from cord blood platelet lysate ..................... 69 V. Results summary ......................................................................................................................... 97 VI. Discussion .............................................................................................................................. 101 VII. Conclusions ................................................................................................................................. 108 VIII. Future investigations ................................................................................................................. 110 IX. References .................................................................................................................................... 112 X. Annex............................................................................................................................................. 129 5 Annex I. Paper 4. Rapid review: next generation of cord blood banks; transplantation and beyond. ....................................................................................................................................................... 130 Annex II. Ethical considerations .................................................................................................... 134 Annex III. Thesis projects development ........................................................................................ 135 1. Public cord blood banks for large scale production of PRP derivatives ............................ 135 2. Concordia Program ............................................................................................................ 135 3. The Research BioBank at Banc de Sang i Teixits ............................................................... 136 4. Spanish regulations for allogeneic PRP and their derivatives ........................................... 137 5. CB-PRP projects development .......................................................................................... 137 6 Abstract Background: There are many advantages to using cord blood (CB) as a source of therapeutic platelet and plasma derivatives for regenerative medicine. These include availability, universal use, young donor source, and virally safe biological material, rich in tissue regenerative factors. CB platelet rich plasma (CB-PRP) derivatives have been investigated as potential therapeutic agents for the treatment
Load more

Recommended publications
  • Umbilical Cord Blood Banking. a Guide for the Parents
    UMBILICAL CORD BLOOD BANKING A guide for parents 2nd Edition 2016 This guide has been elaborated by the Council of Europe European Committee on Organ Transplantation (CD-P-TO). For more information, please visit https://go.edqm.eu/transplantation. All rights conferred by virtue of the International Copyright Convention are specifically reserved to the Council of Europe and any reproduction or translation requires the written consent of the Publisher. Director of the Publication: Dr S. Keitel Page layout and cover: EDQM Photo: © millaf – Fotolia.com Illustrations: © aeroking – Fotolia.com European Directorate for the Quality of Medicines & HealthCare (EDQM) Council of Europe 7, allée Kastner CS 30026 F-67081 STRASBOURG FRANCE Internet: www.edqm.eu © Council of Europe, 2015, 2016 First published 2015 Second Edition 2016 2 INTRODUCTION The collection and storage of umbilical cord blood when a baby is born is becoming increasingly common. The reason is that the cells contained in the The cells contained in the umbilical cord blood have therapeu- umbilical cord blood have tic value for the treatment of malignant therapeutic value for and non-malignant blood disorders and the treatment of blood immune diseases. Cord blood has been disorders and immune used in transplant medicine since the diseases. first allogeneic cord blood transplant was performed in 1988 and, over the last 25 years, this activity has grown rapidly. Allogeneic cord blood transplantation in children has similar survival rates compared to transplantation of haema- topoietic stem cells from other sources (e.g. bone marrow), and results for adults continue to improve. In recent years, the number of cord blood banks offering families to store the cord blood of their babies for possible future private uses against up front and yearly fees has grown.
    [Show full text]
  • Association Between ABO and Duffy Blood Types and Circulating Chemokines and Cytokines
    Genes & Immunity (2021) 22:161–171 https://doi.org/10.1038/s41435-021-00137-5 ARTICLE Association between ABO and Duffy blood types and circulating chemokines and cytokines 1 2 3 4 5 6 Sarah C. Van Alsten ● John G. Aversa ● Loredana Santo ● M. Constanza Camargo ● Troy Kemp ● Jia Liu ● 4 7 8 Wen-Yi Huang ● Joshua Sampson ● Charles S. Rabkin Received: 11 February 2021 / Revised: 30 April 2021 / Accepted: 17 May 2021 / Published online: 8 June 2021 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021, corrected publication 2021 Abstract Blood group antigens are inherited traits that may play a role in immune and inflammatory processes. We investigated associations between blood groups and circulating inflammation-related molecules in 3537 non-Hispanic white participants selected from the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Whole-genome scans were used to infer blood types for 12 common antigen systems based on well-characterized single-nucleotide polymorphisms. Serum levels of 96 biomarkers were measured on multiplex fluorescent bead-based panels. We estimated marker associations with blood type using weighted linear or logistic regression models adjusted for age, sex, smoking status, and principal components of p 1234567890();,: 1234567890();,: population substructure. Bonferroni correction was used to control for multiple comparisons, with two-sided values < 0.05 considered statistically significant. Among the 1152 associations tested, 10 were statistically significant. Duffy blood type was associated with levels of CXCL6/GCP2, CXCL5/ENA78, CCL11/EOTAXIN, CXCL1/GRO, CCL2/MCP1, CCL13/ MCP4, and CCL17/TARC, whereas ABO blood type was associated with levels of sVEGFR2, sVEGFR3, and sGP130.
    [Show full text]
  • Comparison Between Human Cord Blood Serum and Platelet-Rich Plasma Supplementation for Human Wharton's Jelly Stem Cells and Dermal Fibroblasts Culture
    Available online at www.ijmrhs.com International Journal of Medical Research & ISSN No: 2319-5886 Health Sciences, 2016, 5, 8:191-196 Comparison between human cord blood serum and platelet-rich plasma supplementation for Human Wharton's Jelly Stem Cells and dermal fibroblasts culture Hashemi S. S1* and Rafati A.R 2 1 Burn and Wound Healing Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran 2Division of Pharmacology & Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran *Corresponding E-mail: [email protected] _____________________________________________________________________________________________ ABSTRACT We carried out a side-by-side comparison of the effects of Human cord blood serum (HcbS) versus embryonic PRP on Human Wharton's Jelly Stem Cells(hWMSC)and dermal fibroblasts proliferation. Human umbilical cord blood was collected to prepare activated serum (HCS) and platelet-rich plasma (CPRP).Wharton's Jelly Stem Cells and dermal fibroblasts were cultured in complete medium with10% CPRP, 10%HCSor 10% fetal bovine serumand control (serum-free media).The efficiency of the protocols was evaluated in terms of the number of adherent cells and their expansion and Cell proliferation. We showed that proliferation of fibroblasts and mesenchymal stem cells in the presence of cord blood serum and platelet-rich plasma significantly more than the control group (p ≤0/05). As an alternative to FBS, cord blood serum has been proved as an effective component in cell tissue culture applications and embraced a vast future in clinical applications of regenerative medicine. However, there is still a need to explore the potential of HCS and its safe applications in humanized cell therapy or tissue engineering.
    [Show full text]
  • Guidelines for Transfusion and Patient Blood Management, and Discuss Relevant Transfusion Related Topics
    Guidelines for Transfusion and Community Transfusion Committee Patient Blood Management Community Transfusion Committee CHAIR: Aina Silenieks, M.D., [email protected] MEMBERS: A.Owusu-Ansah, M.D. S. Dunder, M.D. M. Furasek, M.D. D. Lester, M.D. D. Voigt, M.D. B. J. Wilson, M.D. COMMUNITY Juliana Cordero, Blood Bank Coordinator, CHI Health Nebraska Heart REPRESENTATIVES: Becky Croner, Laboratory Services Manager, CHI Health St. Elizabeth Mackenzie Gasper, Trauma Performance Improvement, Bryan Medical Center Kelly Gillaspie, Account Executive, Nebraska Community Blood Bank Mel Hanlon, Laboratory Specialist - Transfusion Medicine, Bryan Medical Center Kyle Kapple, Laboratory Quality Manager, Bryan Medical Center Lauren Kroeker, Nurse Manager, Bryan Medical Center Christina Nickel, Clinical Laboratory Director, Bryan Medical Center Rachael Saniuk, Anesthesia and Perfusion Manager, Bryan Medical Center Julie Smith, Perioperative & Anesthesia Services Director, Bryan Medical Center Elaine Thiel, Clinical Quality Improvement/Trans. Safety Officer, Bryan Med Center Kelley Thiemann, Blood Bank Lead Technologist, CHI Health St. Elizabeth Cheryl Warholoski, Director, Nebraska Operations, Nebraska Community Blood Bank Jackie Wright, Trauma Program Manager, Bryan Medical Center CONSULTANTS: Jed Gorlin, M.D., Innovative Blood Resources [email protected] Michael Kafka, M.D., LifeServe Blood Center [email protected] Alex Smith, D.O., LifeServe Blood Center [email protected] Nancy Van Buren, M.D., Innovative
    [Show full text]
  • The Amazing Stem Cell What Are They? Where Do They Come From? How Are They Changing Medicine? Stem Cells Are “Master Cells”
    The Amazing Stem Cell What are they? Where do they come from? How are they changing medicine? Stem cells are “master cells” Stem cells can be “guided” to become many other cell types. Stem Cell Bone cell Self-renewed stem cell Brain cell Heart muscle Blood cell cell There are several types of stem cells, each from a unique source Embryonic stem cells* • Removed from embryos created for in vitro fertilization after donation consent is given. (Not sourced from aborted fetuses.) • Embryos are 3-5 days old (blastocyst) and have about 150 cells. • Can become any type of cell in the body, also called pluripotent cells. • Can regenerate or repair diseased tissue and organs. • Current use limited to eye-related disorders. * Not used by Mayo Clinic. Adult stem cells • Found in most adult organs and tissues, including bone marrow. • Often taken from bone marrow in the hip. • Blood stem cells can be collected through apheresis (separated from blood). • Can regenerate and repair diseased or damaged tissues (regenerative medicine). • Can be used as specialized “drugs” to potentially treat degenerative conditions. • Currently tested in people with neurological and heart disease. Umbilical cord blood stem cells • Found in blood in placenta and umbilical cord after childbirth. • Have the ability to change into specialized cells (like blood cells), also called progenitor cells. • Parents choose to donate umbilical cord blood for use in research, or have it stored for private or public banks. • Can be used in place of bone marrow stem cell transplants in some clinical applications. Bioengineered stem cells • Regular adult cells (e.g., blood, skin) reprogrammed to act like embryonic stem cells (induced pluripotent stem cells).
    [Show full text]
  • Cord Blood Stem Cells Umbilical Cord Blood Transplant for Adult Patients
    Bone Marrow Transplantation (2004) 33, 33–38 & 2004 Nature Publishing Group All rights reserved 0268-3369/04 $25.00 www.nature.com/bmt Cord blood stem cells Umbilical cord blood transplant for adult patients with severe aplastic anemia using anti-lymphocyte globulin and cyclophosphamide as conditioning therapy P Mao, S Wang, S Wang, Z Zhu, Q Liv, Y Xuv, W Mo and Y Ying Department of Haematology, First Municipal People’s Hospital, Guangzhou, China Summary: therapy with immunosuppressive agents.1 For patients without a sibling donor having no response to one or Allo-CBSCT (cord blood stem cell transplant) has more courses of immunosuppressive therapy a fully been applied in sixadult patients with severe aplastic matched unrelated donor BMT should be considered anemia (SAA). Anti-lymphocyte globulin (ALG) asalternative salvagetherapy. Finding related or unrelated 40 mg kgÀ1 dÀ1 Â 3 days combined with cyclophosphamide individualswho are HLA-identical to some (CTX) 20 mg kgÀ1 dÀ1 Â 3 days constituted a lower patients, however, is difficult and time consuming because intensive conditioning regimen. The prophylaxis of of the extreme polymorphism of most HLA GVHD consisted of standard CsA and MTX. Patients loci. Umbilical cord blood isanother alternative sources are all male having a mean age of 26.5 years (range 22– of stem cells that improves donor availability for trans- 38), and a median weight of 55.6 kg (range 52–60 kg). plantation because frozen and stored UCB can be Cord blood searches were all conducted at Guangzhou made available on demand. Infectiousagents,particularly Cord Blood Bank. Three of sixpatients in our study cytomegalovirus(CMV), are rarely seenin the new received one unit of cord blood in a procedure, whereas for born than in adults.
    [Show full text]
  • Comparative Analysis of Human Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Umbilical Cord Blood As Sources of Cell Therapy
    Int. J. Mol. Sci. 2013, 14, 17986-18001; doi:10.3390/ijms140917986 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Comparative Analysis of Human Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Umbilical Cord Blood as Sources of Cell Therapy Hye Jin Jin 1,2,†, Yun Kyung Bae 1,†, Miyeon Kim 1, Soon-Jae Kwon 1, Hong Bae Jeon 1, Soo Jin Choi 1, Seong Who Kim 2, Yoon Sun Yang 1, Wonil Oh 1 and Jong Wook Chang 1,* 1 Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul 137-874, Korea; E-Mails: [email protected] (H.J.J.); [email protected] (Y.K.B.); [email protected] (M.K.); [email protected] (S.-J.K.); [email protected] (H.B.J.); [email protected] (S.J.C.); [email protected] (Y.S.Y.); [email protected] (W.O.) 2 Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Korea; E-Mail: [email protected] † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +82-2-3465-6771; Fax: +82-2-475-1991. Received: 27 May 2013; in revised form: 18 July 2013 / Accepted: 22 August 2013 / Published: 3 September 2013 Abstract: Various source-derived mesenchymal stem cells (MSCs) have been considered for cell therapeutics in incurable diseases. To characterize MSCs from different sources, we compared human bone marrow (BM), adipose tissue (AT), and umbilical cord blood-derived MSCs (UCB-MSCs) for surface antigen expression, differentiation ability, proliferation capacity, clonality, tolerance for aging, and paracrine activity.
    [Show full text]
  • Maternal and Cord Blood Hemoglobin As Determinants of Placental Weight: a Cross-Sectional Study
    Journal of Clinical Medicine Article Maternal and Cord Blood Hemoglobin as Determinants of Placental Weight: A Cross-Sectional Study Ferrante S. Gragasin 1,2,†, Maria B. Ospina 2,3,† , Jesus Serrano-Lomelin 2,3 , Su Hwan Kim 4, Matthew Kokotilo 1, Andrew G. Woodman 2,5 , Stephen J. Renaud 6,‡ and Stephane L. Bourque 1,2,5,*,‡ 1 Department of Anesthesiology & Pain Medicine, University of Alberta, Edmonton, AB T6G 2G3, Canada; [email protected] (F.S.G.); [email protected] (M.K.) 2 Women and Children’s Health Research Institute, University of Alberta, Edmonton, AB T6G 1C9, Canada; [email protected] (M.B.O.); [email protected] (J.S.-L.); [email protected] (A.G.W.) 3 Department of Obstetrics & Gynecology, University of Alberta, Edmonton, AB T6G 2R7, Canada 4 Department of Mathematical & Statistical Sciences, University of Alberta, Edmonton, AB T6G 2G1, Canada; [email protected] 5 Department of Pharmacology, University of Alberta, Edmonton, AB T6G 2H7, Canada 6 Department of Anatomy and Cell Biology, University of Western Ontario, London, ON N6A 5C1, Canada; [email protected] * Correspondence: [email protected]; Tel.: +1-780-492-6000 † These authors contributed equally to this work. ‡ These authors contributed equally to this work. Abstract: Background: Both high and low placental weights are associated with adverse pregnancy outcomes. Maternal hemoglobin levels can influence placental weight, but the evidence is conflicting. Citation: Gragasin, F.S.; Ospina, Since maternal hemoglobin does not invariably correlate with fetal/neonatal blood hemoglobin M.B.; Serrano-Lomelin, J.; Kim, S.H.; levels, we sought to determine whether cord blood hemoglobin or maternal hemoglobin status more Kokotilo, M.; Woodman, A.G.; closely associates with placental weight in women undergoing elective cesarean section at term.
    [Show full text]
  • Cord Blood Stem Cell Transplantation
    LEUKEMIA LYMPHOMA MYELOMA FACTS Cord Blood Stem Cell Transplantation No. 2 in a series providing the latest information on blood cancers Highlights • Umbilical cord blood, like bone marrow and peripheral blood, is a rich source of stem cells for transplantation. There may be advantages for certain patients to have cord blood stem cell transplants instead of transplants with marrow or peripheral blood stem cells (PBSCs). • Stem cell transplants (peripheral blood, marrow or cord blood) may use the patient’s own stem cells (called “autologous transplants”) or use donor stem cells. Donor cells may come from either a related or unrelated matched donor (called an “allogeneic transplant”). Most transplant physicians would not want to use a baby’s own cord blood (“autologous transplant”) to treat his or her leukemia. This is because donor stem cells might better fight the leukemia than the child’s own stem cells. • Cord blood for transplantation is collected from the umbilical cord and placenta after a baby is delivered. Donated cord blood that meets requirements is frozen and stored at a cord blood bank for future use. • The American Academy of Pediatrics’s (AAP) policy statement (Pediatrics; 2007;119:165-170.) addresses public and private banking options available to parents. Among several recommendations, the report encourages parents to donate to public cord blood banks and discourages parents from using private cord blood banks for personal or family cord blood storage unless they have an older child with a condition that could benefit from transplantation. • The Stem Cell Therapeutic and Research Act of 2005 put several programs in place, including creation of the National Cord Blood Inventory (NCBI) for patients in need of transplantation.
    [Show full text]
  • Therapeutic Apheresis, J Clin Apheresis 2007, 22, 104-105
    Apheresis: Basic Principles, Practical Considerations and Clinical Applications Joseph Schwartz, MD Anand Padmanabhan, MD PhD Director, Transfusion Medicine Assoc Med Director/Asst Prof Columbia Univ. Medical Center BloodCenter of Wisconsin New York Presbyterian Hospital Medical College of Wisconsin Review Session, ASFA Annual meeting, Scottsdale, Arizona, June 2011 Objectives (Part 1) • Mechanism of Action • Definitions • Technology (ies) • Use • Practical Considerations • Math • Clinical applications – HPC Collection Objectives (Part 2) • Clinical applications: System/ Disease Specific Indications • ASFA Fact Sheet Apheresis •Derives from Greek, “to carry away” •A technique in which whole blood is taken and separated extracorporealy, separating the portion desired from the remaining blood. •This allows the desired portion (e.g., plasma) to be removed and the reminder returned. Apheresis- Mechanism of Action •Large-bore intravenous catheter connected to a spinning centrifuge bowl •Whole blood is drawn from donor/patient into the centrifuge bowl •The more dense elements, namely the RBC, settle to the bottom with less dense elements such as WBC and platelets overlying the RBC layer and finally, plasma at the very top. Apheresis: Principles of Separation Platelets (1040) Lymphocytes Torloni MD (1050-1061) Monocytes (1065 - 1069) Granulocyte (1087 - 1092) RBC Torloni MD Torloni MD Separate blood components is based on density with removal of the desired component Graphics owned by and courtesy of Gambro BCT Principals of Apheresis WBC Plasma Torlo RBC ni MD Torloni MD RBC WBC Plasma G Cobe Spectra Apheresis- Mechanism of Action Definitions • Plasmapheresis: plasma is separated, removed (i.e. less than 15% of total plasma volume) without the use of replacement solution • Plasma exchange (TPE): plasma is separated, removed and replaced with a replacement solution such as colloid (e.g.
    [Show full text]
  • Case Report Successful Hematopoietic Reconstitution by Transplantation of Umbilical Cord Blood Cells in a Transfusion-Dependent Child with Diamond– Blackfan Anemia
    Bone Marrow Transplantation, (1997) 19, 83–85 1997 Stockton Press All rights reserved 0268–3369/97 $12.00 Case report Successful hematopoietic reconstitution by transplantation of umbilical cord blood cells in a transfusion-dependent child with Diamond– Blackfan anemia M Bonno1, E Azuma1,2, T Nakano1, M Higashikawa1, H Kawaski1, H Nishihara1, M Obata1, M Umemoto1, H Sakatoku1, Y Komada1, M Ito1, M Nagai1,2 and M Sakurai1 1Department of Pediatrics and 2Department of Clinical Immunology, Mie University School of Medicine, Mie, Japan Summary: Case report A 4-year-old boy with Diamond–Blackfan anemia and A 4-year-old boy was first noted to have severe anemia at a history of multiple transfusions underwent umbilical 1 month of age. An evaluation showed hemoglobin 50 g/l, cord blood transplantation from his HLA-identical white blood cell count 5.5 × 109/l with normal distribution, female sibling born by vaginal delivery at 38 weeks. The platelet count 385 × 109/l, and 0% reticulocyte. The bone patient was prepared with busulfan, cyclophosphamide marrow showed marked erythroid hypoplasia with no and antilymphocyte globulin. Methotrexate and cyclo- abnormal cells and a diagnosis of Diamond–Blackfan ane- sporin A were given for the prophylaxis of GVHD. Regi- mia was confirmed. He was 2680 g at birth, the product men-related toxicity was not observed and successful of a full-term, uncomplicated gestation. The parents were engraftment occurred, including the erythroid series. healthy and there was no consanguinity or family history No evidence of acute or chronic GVHD has been of hematological disorders. Several therapeutic approaches observed for 14 months after transplantation.
    [Show full text]
  • Umbilical Cord Blood Hematology in Relation with Maternal Anemia: a Preliminary Study
    Online - 2455-3891 Vol 11, Issue 10, 2018 Print - 0974-2441 Research Article UMBILICAL CORD BLOOD HEMATOLOGY IN RELATION WITH MATERNAL ANEMIA: A PRELIMINARY STUDY RUMI DEBBARMA1*, PANKAJ2, M. ANITA DEVI3 1Department of Maulana Azad Medical College, New Delhi, India. 2Department of Indraprastha Apollo Hospital, Jasola, New Delhi, India. 3Department of Regional Institute of Medical Sciences, Imphal, Manipur, India. Email: [email protected] Received: 19 April 2018, Revised and Accepted: 26 June 2018 ABSTRACT Objectives: The aim of this study was to observe any umbilical cord blood hematological changes in relation to maternal anemia. Methods: We conducted a cross-sectional study on 220 neonates and their mothers from August 1, 2015, to July 31, 2016, in collaboration with the Department of Obstetrics and Gynaecology, Regional Institute of Medical Sciences, Imphal, India. Immediately after vaginal delivery of baby, umbilical cord was clamped and blood was collected in EDTA vials and analyzed using automated hematoanalyzer. Results: Neonates of non-anemic mothers had significantly higher level of hemoglobin concentration than neonates of anemic mothers. Total leukocyte count (TLC) was slightly higher in neonates of non-anemic mothers as compared to neonates of anemic mothers. Platelet count was slightly higher in neonates of anemic mothers as compared to non-anemic mothers, but platelet crit, mean platelet volume, and platelet cell distribution width were slightly higher in neonates of non-anemic mothers than neonates anemic mothers. Conclusions: It was a preliminary study. A similar type of the study must be conducted in future to comprehend the effect of various other factors affecting delivery such as maternal anemia, diet, and environment on blood parameters of newborns.
    [Show full text]