Supplemental Data

Figure Captions

Figure 1: Photographs of the MALDI plate of the first experiment of on-target homogenization/transfer of multiple samples employing the TissueLyser approach: before (A) and after (B) excess material was removed .

Figure 2: Photographs of MALDI plates of the first experiment of on-target homogenization/transfer of multiple samples employing 25 Eppendorf tubes and the vortex device for (I.A) 1 min. and (II.A) 5 min. as well as their respective (B) enlargements.

Figure 3: Mass spectra of PMMA 21,000 narrow PD standard (top) using Method 3a for simultaneous homogenization and transfer to the MALDI plate with dithranol/NaCl matrix and (bottom) dissolving the solvent free matrix preparation in tetrahydrofuran.

Figure 1

Figure 2

Figure 3 10 217. 1 09 80 70 % 60 In 50 te n 40 si 30 ty 20 10 00 500 1000 1500 2000 2500 3000 0 0 0 Mass 0 0 0 10 (m/z) 893. 2 09 80 70 % 60 In 50 te 0 n 4 si 30 ty 20 10 00 500 1000 2000 2500 3000 0 0 Mass 0 0 0